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<entry dataset="Swiss-Prot" created="2002-05-02" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>P58813</accession>
    <name>APGM_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="1">BPG-independent PGAM</shortName>
            <shortName evidence="1">Phosphoglyceromutase</shortName>
            <shortName evidence="1">aPGAM</shortName>
            <ecNumber evidence="1">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apgM</name>
        <name type="ordered locus">MK1193</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58813"/>
    <dbReference type="SMR" id="P58813"/>
    <dbReference type="STRING" id="190192.MK1193"/>
    <dbReference type="EnsemblBacteria" id="AAM02406">
        <property type="protein sequence ID" value="AAM02406"/>
        <property type="gene ID" value="MK1193"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1193"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1295"/>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="OMA" id="IAFRCNF"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138136" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="422" mass="45625" checksum="30BAC62C9DF15B45" modified="2002-05-02" version="1">MRVTGLERKILVIVGDGMADRAVPELDGKTPLQAADTPNMDRLAREGSVGLLDPIRPGVRPGSDTAHLTLLGYDPFEVYPGRGPLEALGAGVEVRPGDVAFRCNFATAEERNGELVVVDRRAGRINEDEGTPKLAETINEEVDLPVEFEFKEAVGHRAVLVLRGGDLSADVTDADPKRVGKPVKDVKPTSDDPAAARTAEIVNEFVRKAYEVLKDHPVNRERERQGKPPANVILPRGAGQLEEVEPFSDRYGMSGAVVAGASLIKGIGRMLGMDVPEDEAITGRKDTDLKRKAELALEALDDHDLVLVNFNAVDEAGHDGDARGKVEMIERMDRELVGTLLEGIDPEETVVCLTADHSTPVAVGDHTADPVPVAIWTADARRDPVEEYDEISAARGCLGRFSGLHLLNVLRDLADRIEKFGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT9</accession>
    <name>TPIS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Triosephosphate isomerase</fullName>
            <shortName evidence="1">TIM</shortName>
            <shortName evidence="1">TPI</shortName>
            <ecNumber evidence="1">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tpiA</name>
        <name type="ordered locus">MK1664</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUT9"/>
    <dbReference type="SMR" id="Q8TUT9"/>
    <dbReference type="STRING" id="190192.MK1664"/>
    <dbReference type="EnsemblBacteria" id="AAM02877">
        <property type="protein sequence ID" value="AAM02877"/>
        <property type="gene ID" value="MK1664"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1664"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1828"/>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="OMA" id="IPVYAQH"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_A">
        <property type="entry name" value="TIM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022891">
        <property type="entry name" value="Triosephosphate_isomerase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090336" description="Triosephosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <sequence length="226" mass="23282" checksum="04BA4FC926EECB1A" modified="2002-06-01" version="1">MLRVPPVIVNFKAYSEAVGENALRLARVAAEVSEETGVEVGICPPHVDLRDVVREVGDEVTVLAQAVDAAEPGGRTGHVTPEMVVEAGADGTLLNHSERRMLLEDLKDVCRACINEGLLTIVCASDALAARAAGALSPHAVAVEPPELIGTGTPVSKADPEVVERSVEVVKEVSEETAVLCGAGITDGSDVRAAVELGADGVLVASGVVLADDPKEALLDLISGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX9</accession>
    <name>Q8TUX9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-39 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02836">
        <property type="protein sequence ID" value="AAM02836"/>
        <property type="gene ID" value="MK1623"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1623"/>
    <dbReference type="HOGENOM" id="CLU_668375_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="411" mass="46311" checksum="A2D92954B9BCECA8" modified="2002-06-01" version="1">MRNSNTPERGDSTLHTWLLLWLLLPMTPAQADPTSHYLEVHPDTVPVTPEGSPLVPAYVELTDEGKEAALSLLERLGIPSDEAAVGYLADVFVGESCHGGTRCLVVYNPQDTTVEVRPWEEDRLPLRLPPLRLDDHLTELLLCLPVREQITLQPAVLELGWNLEMMQRCTKAVRLHKFGEPTAVIAVYVPTETAALRAFESLLPDPRNVARAMLERLSSEVRDAIDEYLVRDRLERLVKSRLGSDVYDRVREYFSSMIHLTVSKIEELYDERVLPALIEWCAAIVRYVETSLITAFALLYPDNPSADVLERRVAVYNSPYFQILRDWLLEPSYTAWKSVLCDLAVTVADSIFEAARPVLSALRLDSESSRTTLEEITKSAVKNFIEALGPCLRTVLELLLLDTLMGLAWAC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV15</accession>
    <name>Y1586_METKA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0147 protein MK1586</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1586</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0147 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02799" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02799.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV15"/>
    <dbReference type="SMR" id="Q8TV15"/>
    <dbReference type="STRING" id="190192.MK1586"/>
    <dbReference type="EnsemblBacteria" id="AAM02799">
        <property type="protein sequence ID" value="AAM02799"/>
        <property type="gene ID" value="MK1586"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1586"/>
    <dbReference type="HOGENOM" id="CLU_165882_0_1_2"/>
    <dbReference type="OMA" id="RTIIWEV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150909" description="UPF0147 protein MK1586">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="104" mass="11582" checksum="36C25A3CCAD5F143" modified="2002-10-10" version="2">MSEARQQSSSEGGGDLSDYEEKFEQCCTLLEQRIIQDDQIPRNVRRAAKQAIEALKEEGQSPGVRASTAISTLEEVVNDQNTPEYARTVLLQVIATLEQVKDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV19</accession>
    <name>Q8TV19_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1582"/>
    <dbReference type="EnsemblBacteria" id="AAM02795">
        <property type="protein sequence ID" value="AAM02795"/>
        <property type="gene ID" value="MK1582"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1582"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1743"/>
    <dbReference type="HOGENOM" id="CLU_067259_0_0_2"/>
    <dbReference type="OMA" id="CGIDEMV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DUF1611-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DUF1611_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="UCP026760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="PTHR40690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1611_N" evidence="2">
        <location>
            <begin position="41"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1611" evidence="1">
        <location>
            <begin position="150"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="353" mass="39146" checksum="5E037AB489F726B4" modified="2002-06-01" version="1">MKPLEHPEVTRAVMVPFTKVAAGILRFADIEIVGICDYREEKVGKTTKELVGDDVPELEIRPMEDLEELLEEAEWLIWTKEVLDEDVHYLKWREAVDEAVVKGVNVYNMGRLHMVAQDPNWKLEAHKRGVQYFDTSDPELYLEYLHYGMKAREEGVNADVVSIVGTGRRTGKFTTLNTARRILEEEGINVRSVGTEPSSLLTGSEAMVIPQVLPMAHAAGTVYGAVKKLDEEYEPDLILVGAQTGVTADPLEVGTGRGGSLAALTILLGSDPDRMVVATRPELLNGLQDVVEVVTLLTGARVQFVSVNGKDYSEEEVREVCERIEDEFGLPAADPIKMEEEFRDLVLDLIAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV21</accession>
    <name>Q8TV21_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit K/omega</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpoZ</name>
        <name evidence="3" type="ordered locus">MK1580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1580"/>
    <dbReference type="EnsemblBacteria" id="AAM02793">
        <property type="protein sequence ID" value="AAM02793"/>
        <property type="gene ID" value="MK1580"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1580"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1741"/>
    <dbReference type="HOGENOM" id="CLU_112527_5_0_2"/>
    <dbReference type="OMA" id="HNRYEKA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="56" mass="6235" checksum="828B2CC3D29F4635" modified="2002-06-01" version="1">MEDLTRFEIARIVGARALQIAMGSPVLVEVEGDEDPLEIAKREFDEGVVPVVVIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV22</accession>
    <name>Q8TV22_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1579"/>
    <dbReference type="EnsemblBacteria" id="AAM02792">
        <property type="protein sequence ID" value="AAM02792"/>
        <property type="gene ID" value="MK1579"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1579"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="OMA" id="CLSCHEI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12837">
        <property type="entry name" value="Fer4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="63" mass="6691" checksum="55DDC3C8529EEC21" modified="2002-06-01" version="1">MPKVVIDYDACVGVSECGECIEACPMDVLDEEDDKPVVVNEDDCTGCGLCEQACPHGAIEVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV52</accession>
    <name>Q8TV52_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uroporphyrinogen-III methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cysG_2</name>
        <name evidence="5" type="ordered locus">MK1548</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1548"/>
    <dbReference type="EnsemblBacteria" id="AAM02761">
        <property type="protein sequence ID" value="AAM02761"/>
        <property type="gene ID" value="MK1548"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1548"/>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="OMA" id="TRPEQEE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="26418" checksum="975B846B70611643" modified="2002-06-01" version="1">MAGKLVLVGAGPGDPELLTFKAARAISRGDVILKDRLVPDEIIKEHAPEDAEVIDVGKKPGGEGWTQEEINELIVREGSKGKTVVRVKSGDPLIFGRGAEEIEVALKHGMDVEVVPGVTSAIGVPTSLGLPLTHRKCASSFAVATGHEDPSKPENRVDFGALAEAADTLVVLMGARRLREIAREILEKRGNEPVAILERGTTEQERVKVGTLEDAAEGKLKARPPAVVVVGEVVKWWREVLGRETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV56</accession>
    <name>Q8TV56_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypE_2</name>
        <name evidence="4" type="ordered locus">MK1544</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1544"/>
    <dbReference type="EnsemblBacteria" id="AAM02757">
        <property type="protein sequence ID" value="AAM02757"/>
        <property type="gene ID" value="MK1544"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1544"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1705"/>
    <dbReference type="HOGENOM" id="CLU_049733_0_0_2"/>
    <dbReference type="OMA" id="CGNGGKE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="41"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="162"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="332" mass="34969" checksum="D4715C0FCB12A7F9" modified="2002-06-01" version="1">MSKVRREHGAGGELMESLIKEELLPNLTMRGEGSVTLDDLDDGATFPSVDGEMVMTTDAHIVDPPFFPGGNVGKLAAAGTANDLAVMGAKPVAFACSIVVREGFPIDDLKRVYRSIDGVLSELGAHLITGDTKVGNTGDVDIVVTMTGVGEIVELVRDCGLRPGDKIVITGTVGDHGMAILAAQQGLDTDLESDVAPVWEAVNAALEVGGVTSMKDPTRGGLAGALNEMAEKSGVRIVIEEERIPIREEVRVLSEMLGVNPLEVANEGKVVMGVRPDMVDDVLDAIRSTEVGKNAEVIGVVEEGTPRVEMETEVGGRRIVEKPVGDPVPRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV57</accession>
    <name>Q8TV57_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02756">
        <property type="protein sequence ID" value="AAM02756"/>
        <property type="gene ID" value="MK1543"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1543"/>
    <dbReference type="HOGENOM" id="CLU_1118219_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="248" mass="27190" checksum="E6076ABFEA9F6642" modified="2002-06-01" version="1">MRVSPSEGARRVWRAIKTELSEHGPELGRLEEELSAAVPEDAEVKEIRAYGYRRSVGIFRRINRLWILIRGDESVAKDMERNLIDRIVEDVEDPKVRHKLNWALRGALRVTTIDDVRPEPEVIRRVTTLCALCGAASVVLLGIEALAYPKRVVKLLPPAIAVSGAAGCLLPWRVGLLGLADDALDHPRRVTVPVAVSLTLLVLAGLVSSLLLPYLGTGFLGVVIAACVTGFVAARFVELINREVVKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV75</accession>
    <name>Q8TV75_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobalamin biosynthesis protein CbiG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cbiG</name>
        <name evidence="2" type="ordered locus">MK1518</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02731">
        <property type="protein sequence ID" value="AAM02731"/>
        <property type="gene ID" value="MK1518"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1518"/>
    <dbReference type="HOGENOM" id="CLU_2044425_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiG_C" evidence="1">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="120" mass="13384" checksum="82B62FC50FE6321F" modified="2002-06-01" version="1">MYALGVGMRRRVRRQDLVTAVRWGLEEAGELGEIITITRKRDQPSGKALVEIGRRFGIPVRFVEKLADHTPSDSRARDLLGAPGSVCEGVLITHGYEIVRPKRTFGNTVTAALGWKRSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV90</accession>
    <name>NIKR_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1502</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="1">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV90"/>
    <dbReference type="SMR" id="Q8TV90"/>
    <dbReference type="STRING" id="190192.MK1502"/>
    <dbReference type="EnsemblBacteria" id="AAM02715">
        <property type="protein sequence ID" value="AAM02715"/>
        <property type="gene ID" value="MK1502"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1502"/>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="AHNCLET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000139303" description="Putative nickel-responsive regulator">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <sequence length="141" mass="16123" checksum="4471513C8F458DCA" modified="2002-06-01" version="1">MNKGEDNLVRTSITVPEQLLQKVNELIASGYFASRSEIFRQALREYLQRIEWTERVGDEEYFGALTYVFQHERAEPELVKVQHEFTDVIISTTHIHVSPEKCLEVLLLQGPGKRIAELAKRIRGVRGVEQAKLTVVSSEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV95</accession>
    <name>Q8TV95_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02710">
        <property type="protein sequence ID" value="AAM02710"/>
        <property type="gene ID" value="MK1497"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1497"/>
    <dbReference type="HOGENOM" id="CLU_803466_0_0_2"/>
    <dbReference type="OMA" id="LFEHHTR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014846">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08735">
        <property type="entry name" value="DUF1786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029129">
        <property type="entry name" value="DUF1786_pyruvate_format-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="355" mass="40258" checksum="45F60EC84D2B7979" modified="2002-06-01" version="1">MEPVSFRGEVKTVVIDVGVGTTDVVAYTGDPEYSPRFVGPSRVSTLAQRLRFMLREPPRYLALIGVPMGGGPTTREFKRLMKKGTEIYAELDAALTLHNDVRRLEEMPRLHIVEDPIEEVPPDSPVVETYDFRVSDVFEALQRSNVDMDGVETVVACVQDHGYHPDYESNRKHRFERLFRRYLGANGCRPDRMTFDDVPPESFPRLRAAYMEAESAGVDAVAMDSKVPIAMLGRVDSDADRLLVIDYGTGHVTAFLFDGDRIVGVYEHHTIRLSSEKFERQIREFVEGKLENEDVYRDGGHGCHNVSPMDWDELEDIVSLGPKKPEFQLGRDPERFPDRMMPAYGPAVYLTDRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB3</accession>
    <name>Q8TVB3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-39 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1479</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02692">
        <property type="protein sequence ID" value="AAM02692"/>
        <property type="gene ID" value="MK1479"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1479"/>
    <dbReference type="HOGENOM" id="CLU_668375_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="453" mass="49913" checksum="7693E5AFE133ACE9" modified="2002-06-01" version="1">MRAVATLLTCMLALTGAASAGKYFEVTQTPVVVSLQGVPLSDVRVKFTDEGKQVVENILSGLKVKVDSEEVQVTNVARKIAEAREGNITNEEYVVGALGYEVPEGKYFVGYLQVKSGEGETKKYVVRYEEEQKAVIKSAPGFGSFTATVVQVLGEYDDKGLPLMTPSIDPTEVKDEVLLCLPVIKEITLKPAVLQMDWYKILEKAEPGKVFVKLAQVHAHGEGDKIEVIYAPTKVDALQLLVSGEEPLIPTQEDMVSTIVDPYMEELEAVVDTKIDEFLKPIQEKVQGNPALEILTERFLPTFLKTLAGKQILRVKETIVSALMPHTQYVRTFLVGLYMSQYPDGVNVNTLMARKAAYESPMYEILVSAVKTSYDMMLESLKSTLSNIIDSIVDQYADEIAEMVGTTTEDVKQTVEKLTVGVLKDLLTGDYVDYLIKLVALNVLTSMGSMGIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE5</accession>
    <name>IF2G_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="1">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2g</name>
        <name type="ordered locus">MK1447</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE5"/>
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        <property type="entry name" value="G_TR_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
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    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="412"/>
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        <location>
            <begin position="20"/>
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        <ligand>
            <name>GTP</name>
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            <position position="20"/>
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            <name>GTP</name>
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    </feature>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <sequence length="412" mass="45776" checksum="9AA81521F0E35047" modified="2002-06-01" version="1">MDDERFQQAEMNIGMVGHVDHGKTTLTKALSGVWTDTHSEETRRGISIRLGYADTVLTRCPECDTYSVEEKCPECGAETEWLRRVSFVDSPGHETLMATMLSGAAIMDAAILVIAANEPCPQPQTREHLMALEIIGTEDVIVVQNKIDLVTPEEAREHYEQIVQFLEEETHLDPDKTPIIPVSAQHKANLDVLVEAMYEHFEPPEYDLDAPFRMYIARSFDVNKPGTRPSDLKGGVIGGAIVQGEVEIGDEIEIRPGIRVERYGRTEWEPVYTEVVSLHANVTPVERARPGGLVGIGTKLDPTMTKADRLSGQVAGEPDTLPPVRHELLLEVELLERVVGTEEERKVEPIRTNEVLMLTVGTATTVGVVTSARDDEIEIKLKQPVCAEEGDRVAISRRIQRWRLIGHGVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF0</accession>
    <name>Q8TVF0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">radA_2</name>
        <name evidence="6" type="ordered locus">MK1442</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAM02655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="protein sequence ID" value="AAM02655"/>
        <property type="gene ID" value="MK1442"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1442"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1598"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rad51_DMC1_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
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        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
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    </dbReference>
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        <property type="entry name" value="Rad51_DMC1_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
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        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
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        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="73"/>
            <end position="244"/>
        </location>
    </feature>
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        <location>
            <begin position="261"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="317" mass="35543" checksum="3CE80FA44CB52EFE" modified="2002-06-01" version="1">MKERKLSDLDLLPDETVKKLEEKGIVTVEDFIYADPKYLSEVTGMSERDVEDIQEELRNIDVEFETLEKLERKRRRITTGSSALDEILGGGVPCGELTEFAGPFGSGKSQIVFQLCVNVQLPEEEGGLESKAIFIDTEGTVSPGRIKGMAEALGLDPGEALRNVFVTQVRSVEEQMRAAEEAHKLCEREDIGLVVIDSLTAHFRAEYSKLGDVSERQARLMKHVDQLRNLAMDHDVAVVFTNQVHVDIEAATKGKGRRYEPVGGTIVAHQATHRIMLRRAKGEVRIARIIDSPYLPQREAAFRITEEGIRDVEFPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF1</accession>
    <name>Q8TVF1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Replication factor A (SsDNA-binding protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1441"/>
    <dbReference type="EnsemblBacteria" id="AAM02654">
        <property type="protein sequence ID" value="AAM02654"/>
        <property type="gene ID" value="MK1441"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1441"/>
    <dbReference type="HOGENOM" id="CLU_634034_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="91"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="1">
        <location>
            <begin position="189"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="432" mass="48237" checksum="BB18E64D6A0F12A2" modified="2002-06-01" version="1">MPRSRGAILTSDIDRLVEKLAEEVGKREEEVRREINRLRKRWGVSELGALLALADRMGVKLMRTGEEKPGRVTLDEAISRGLQSFDTEFIVVRVSDPAKTRSGGKMVTLVVGDETRSAALVAFDEAVETLEELEEGDVVRARNLTVSSFRNSPQLVVTRETELEVVGSEEDPNRIIERNISEVKHGEYVRVRGVVASEPVDTGERVYFWLSDETGSTRVNLWGEEAERALDLDYGDGVIVEGWVSTRGDHPTINILRTQGRVEPAEVSIKPAIRKRVEELGKGDVAEVSGVIVAVYARRRYYEACPTCGRAMRKGECPEHGAVEPERRPVLNVVVDDGTGTVRTVFFGEHAVEFAGYETTREYLEADESDIEKRLLGESVSVVLRVRGEGVVEDYDAVALRARILNEEDFKREIPILVRELKGEESEGEEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF7</accession>
    <name>Q8TVF7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription factor homologous to NACalpha-BTF3 fused to metal-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1432"/>
    <dbReference type="EnsemblBacteria" id="AAM02645">
        <property type="protein sequence ID" value="AAM02645"/>
        <property type="gene ID" value="MK1432"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1432"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1588"/>
    <dbReference type="HOGENOM" id="CLU_1727260_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017671">
        <property type="entry name" value="Methan_mark_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03277">
        <property type="entry name" value="methan_mark_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="174" mass="19360" checksum="7E311509819DC3FB" modified="2002-06-01" version="1">MNVVKEGWPDAPSHVCRGGPPEALAFCCPPVKPCPIFHALDEAGLDPEEYVRRKKEFAEKTPLGSGKNTCFGSLVWCCKITKPCPLRDSTLQRIGMSPEEYMWWKKKLAEYLLGKKDLDEILRETSESEPEEETVEVVAEAAGVSEEEARRALEEANGDPVRAVKLLKSRGKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG4</accession>
    <name>Q8TVG4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1425"/>
    <dbReference type="EnsemblBacteria" id="AAM02638">
        <property type="protein sequence ID" value="AAM02638"/>
        <property type="gene ID" value="MK1425"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1425"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1581"/>
    <dbReference type="HOGENOM" id="CLU_082100_0_0_2"/>
    <dbReference type="OMA" id="KSYIAVN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011672">
        <property type="entry name" value="DUF1614"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07758">
        <property type="entry name" value="DUF1614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="241" mass="25389" checksum="68D47C0B8096C759" modified="2002-06-01" version="1">MTVLNSHVVHSPTSRTFLLFLAAWLLLSFLMMFLYFVSIPGFFHALGLEPRTALLLSLLSIVGSAVNVPIKRIRKLVTVQHETYGFWGISYQVPVRRSEEIVIAVNVGGCLIPVAVSVYLIATNLDLWLQYLLATAVTTIVSYATARVIPGVGIAVPFFLPAAVAGTVALLTTKGGAASVAYVAGTLGTLIGADLLNLRKAVNWGSAPVLSIGGAGTFDAVFVTGLTAVWIAYVLSPGAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK5</accession>
    <name>Q8TVK5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1384"/>
    <dbReference type="EnsemblBacteria" id="AAM02597">
        <property type="protein sequence ID" value="AAM02597"/>
        <property type="gene ID" value="MK1384"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1384"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1539"/>
    <dbReference type="HOGENOM" id="CLU_179754_1_0_2"/>
    <dbReference type="OMA" id="VVHSIDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="PTHR42240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="SSF160363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="96" mass="10627" checksum="2EB7CB00B27CF586" modified="2002-06-01" version="1">MAKIRRLVLDVMKPMEPDTTELARSLAKLEGVDGVNIVLVEVDRDVENVKVTVEGPDLDFERIKDLIEDMGGAIHSIDEVVAGSRIVEEVKTPQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN7</accession>
    <name>Q8TVN7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1351"/>
    <dbReference type="EnsemblBacteria" id="AAM02564">
        <property type="protein sequence ID" value="AAM02564"/>
        <property type="gene ID" value="MK1351"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1351"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1497"/>
    <dbReference type="HOGENOM" id="CLU_002017_1_2_2"/>
    <dbReference type="OMA" id="WPETIAC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1190" mass="135879" checksum="C72C58331EC58669" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP6</accession>
    <name>Q8TVP6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-31 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02555">
        <property type="protein sequence ID" value="AAM02555"/>
        <property type="gene ID" value="MK1342"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1342"/>
    <dbReference type="HOGENOM" id="CLU_1590902_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="18208" checksum="705E0750DA8A96C4" modified="2002-06-01" version="1">MSPTATRPLETADLVPGTRARGPRHVAEVRAHGAVVVSLPLPLAQIEWILALFGPRYDIFGALDTLLRVLRTLLAVPSNASVVLSALLPTLPYLLPVPPWLERDVDGFHLDPLLRQTVVPGQPPQSRPQEAGARVVPAPHDRGPQGIPGRRVRSTGTYPCRRFLRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS3</accession>
    <name>Q8TVS3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1315</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02528">
        <property type="protein sequence ID" value="AAM02528"/>
        <property type="gene ID" value="MK1315"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1315"/>
    <dbReference type="HOGENOM" id="CLU_1536672_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09647">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="174" mass="19968" checksum="CB36CF55E4ABD569" modified="2002-06-01" version="1">MGADLRPAELAEEYLYKPGRLDRNRREDAERRFERLLNDFKAKRIQLKPSDDGLLELAFFTALVSLNVSNSQLRRLYAEITNVRNETRRAREGKGSWEDVVAALGKARVVLAYTKGRQGKEFEGLYEVLDEALRKATKIVEDSEDDDVRRRAIEALHFLAEGIVAFHRFLGGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT2</accession>
    <name>Q8TVT2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1306</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1306"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02519">
        <property type="protein sequence ID" value="AAM02519"/>
        <property type="gene ID" value="MK1306"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1306"/>
    <dbReference type="HOGENOM" id="CLU_009583_32_1_2"/>
    <dbReference type="OMA" id="RSLMWIW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022622">
        <property type="entry name" value="DUF3492"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11997">
        <property type="entry name" value="DUF3492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF3492" evidence="2">
        <location>
            <begin position="6"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="304"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="491" mass="56096" checksum="46842CBFE0DE2DE2" modified="2002-06-01" version="1">MLGGPEVTIVTEGTYPIALGGVTTWVQRLIEHSPNVRFNVLCLAPPGKTEPVVDIPENVRDVVVRELVPRRMGKRRWAARVFPVHRIRWLLGRRGEWAPRGFRVLERAFEYILEGEPLEEEMLKRLYEVSENPVKVLEGPTVYTLARYVEDQLGSDERFSDLYWAVSNVCSFVMGAASGVRHMPECDVAHPQNCGVCGFLCAIRKAVKGTPYIITEHGVLIRELDTRLEGLGDVARELYRRCFESMIETSYRYCDEILAISDYHREHAVKQGAPEDRIDVIYSGIETWRFPPPSDVERKFREADRLHVGTVARVEPIKGIDVFVRMAARVAKEMGHDRVRFHVVGPIDDREHYERCREIVERRGLERVVKFHGSQSPEEILRFYHKFHVFVLSSRSEGLPMALLEAMSTGCPVVASEVGAVPYIVKEGIGRTFPAEDHEAGARALLELLQDPEALLRMSYTATREARRFDVTRMCEEYTRRYAKYAVPDPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY6</accession>
    <name>Q8TVY6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1252</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02465">
        <property type="protein sequence ID" value="AAM02465"/>
        <property type="gene ID" value="MK1252"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1252"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="225" mass="25175" checksum="1D5BF19C9204B37E" modified="2002-06-01" version="1">MVGSHAHIPIPHDPVERIRTLRVLREVYRRGRKPSLEVTYRTVNGSTCGPYYVARWRRDSRFKHGRTLYLGKSENESVSFVEWLVSLDRGEVLELARHLMRNLRSVLKTLLTEVSDLPYKKVRRVLARGLALAFDARPAGSPGIRDVLEELPDRLESFVIRTLGGWPAHYSSYLESSVRAGDSSTGGTRYPTWSWNSSAGSCGTAGDASKSVHVLSSTNAHVDIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW20</accession>
    <name>RL14_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14</name>
        <name type="ordered locus">MK1218</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW20"/>
    <dbReference type="SMR" id="Q8TW20"/>
    <dbReference type="STRING" id="190192.MK1218"/>
    <dbReference type="PRIDE" id="Q8TW20"/>
    <dbReference type="EnsemblBacteria" id="AAM02431">
        <property type="protein sequence ID" value="AAM02431"/>
        <property type="gene ID" value="MK1218"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1218"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1321"/>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_L14P_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000266602" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="133" mass="14366" checksum="9204CB5692A3A5C1" modified="2002-06-01" version="1">MKAIKAKSPHAALPVGARLVCADNTGARELQIIAVKGYKGVRRRLPNAGIGDMVVCSVKEGTPDMRKEVVNAVIVRQRKEYRRPDGTRVKFEDNAAVIVTPDGAPRGSEIRGPVAKEAAERWPRIGSIASIIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW21</accession>
    <name>RS17_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17</name>
        <name type="synonym">rpsQ</name>
        <name type="ordered locus">MK1217</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW21"/>
    <dbReference type="SMR" id="Q8TW21"/>
    <dbReference type="STRING" id="190192.MK1217"/>
    <dbReference type="PRIDE" id="Q8TW21"/>
    <dbReference type="EnsemblBacteria" id="AAM02430">
        <property type="protein sequence ID" value="AAM02430"/>
        <property type="gene ID" value="MK1217"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1217"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1320"/>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="OMA" id="KKYERYE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_S17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_S17_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_S17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000232608" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="118" mass="13935" checksum="E243E8CB96C4FFEE" modified="2002-06-01" version="1">MTGEYRMAKDIGLGVKPPRRECDDPNCPFHGNLRVRGMILEGVVVSDRMDKTVIVEREYYRYDRKYERWERRRSRIPAHNPPCIDAQEGDKVRIAECRPLSKTKSFVVIEVLERAQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW45</accession>
    <name>Q8TW45_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1191"/>
    <dbReference type="EnsemblBacteria" id="AAM02404">
        <property type="protein sequence ID" value="AAM02404"/>
        <property type="gene ID" value="MK1191"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1191"/>
    <dbReference type="HOGENOM" id="CLU_1357921_0_0_2"/>
    <dbReference type="OMA" id="MENEPSF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="63"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="201" mass="22199" checksum="E3F6A258D55C5E91" modified="2002-06-01" version="1">MAIPMHAIPSRVEGIELGPLGVEVEILRESHGLLYPWARLLSETSFAVIIAVLGWTYLRDRRTSYVLATTVVGTLVVTVVLKGLIGEPRPFVLHVVSPLTNPDEPYGSFPSGHTSRSFALAAAYHLERRDALTAILWIWAALVGCSRVVLGVHWPHDVIGGALVGITVAVATHRTARLWVRFLSIVDPLARGVRAWRWYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW65</accession>
    <name>Q8TW65_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted membrane-bound metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1171"/>
    <dbReference type="EnsemblBacteria" id="AAM02384">
        <property type="protein sequence ID" value="AAM02384"/>
        <property type="gene ID" value="MK1171"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1171"/>
    <dbReference type="HOGENOM" id="CLU_1237927_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="223" mass="25083" checksum="B3BFF55464513554" modified="2002-06-01" version="1">METSRVRFLRYRTHLLLTYVIVAYLWDPKHALAAALGSLLPDLDHPSSVASRALVPLEKATLLPLRRLINTVVGHRGILHWPVPWALGAFLAWWVGHTGLAAVLLGGCLHCLEDALTVRGVPLLVRRDGGRWRVWNLRLTPVPSDKWDAVLPPATVLLLCLVILACPSEFHTLGLDRIPGLEKVYWTIHERVYHRLLHPFDAYVGTFAREILRLEPMAAFAPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW71</accession>
    <name>Q8TW71_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02378">
        <property type="protein sequence ID" value="AAM02378"/>
        <property type="gene ID" value="MK1165"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1165"/>
    <dbReference type="HOGENOM" id="CLU_881709_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="315" mass="33968" checksum="1BB9B17E6193F203" modified="2002-06-01" version="1">MATIRFVVDGIVVEGNGGRLEFDPKTTLMKGDAASLAGFLVWLDGLFEAGVRLVRRMAEGVEPVVPEGAEPWVEPPLTLPVSGEYSRVELKGLIAGIVESTKDPWTVASRAARITEYLSDKGAVRILHGSDPYDVVAVGVLALKDGAVRGTLETVRSTVETIAEGARDLIREDEGRADEVISGIVGNVLLRLGYYAVNVRVTDRDGRVHIVRDPSTHADRVAVGVPLTGEVLYGTLVPAAEVGLRCLERRDWVVNAIIAERIRRLGLRTVSGSQVVTIDGETLRGDELEESATEILEEGDVERGSRLERPSEPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW88</accession>
    <name>Q8TW88_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri with repeats, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02361">
        <property type="protein sequence ID" value="AAM02361"/>
        <property type="gene ID" value="MK1148"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1148"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1225"/>
    <dbReference type="HOGENOM" id="CLU_243108_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1369"/>
            <end position="1475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1505"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1396"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1416"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1446"/>
            <end position="1462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1531"/>
            <end position="1545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1632" mass="180940" checksum="672D2FB6111A602E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW98</accession>
    <name>Q8TW98_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1138"/>
    <dbReference type="EnsemblBacteria" id="AAM02351">
        <property type="protein sequence ID" value="AAM02351"/>
        <property type="gene ID" value="MK1138"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1138"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1210"/>
    <dbReference type="HOGENOM" id="CLU_2695794_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="73" mass="8302" checksum="8328F0666C6B9AFE" modified="2002-06-01" version="1">MRRLRKKLLERAEGSLEAARWLLGRGNTDLALVMAERAAGLRIRAAQILLAGEHARGHDLRDLRGRAHRRVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC1</accession>
    <name>Q8TWC1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri with repeats, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02327">
        <property type="protein sequence ID" value="AAM02327"/>
        <property type="gene ID" value="MK1114"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1114"/>
    <dbReference type="HOGENOM" id="CLU_738915_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="374" mass="39381" checksum="11C71A16E7C6A9B0" modified="2002-06-01" version="1">MRVLVMAIVAVAMVANSASAITGKATTNGATPEEMLENLRVHVVAEEGATLDLKEHPVVVGEHAVYVVMITEGDAKSSGETYEEQPLLVPLPKDGVRVEGDTLTVTRPVDTIGYCLSPGSAVPGWNGPESLVVPTWEGLSSKLAIAVKPDKIEQVLATGVCEPEWVKYVRITPTTVRRAVRILDSNVEWTRYFSWDAQLKIWDALWPTESGSSLTELLPKLLPTAVLALPLIAYLTTQYLTSHAEGTFSPGPTGSTVQSVNPGSPQETSEAQRETQQEVIESPSGGEETPTITTITESTPSETIEAHFAAVKGGPTTRVSAGSSVGGATGRPVRTGTSGVCVGEHSTGTPPLLPILAVLAATTGLLLCRKGLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD4</accession>
    <name>Q8TWD4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-32 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02314">
        <property type="protein sequence ID" value="AAM02314"/>
        <property type="gene ID" value="MK1101"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1101"/>
    <dbReference type="HOGENOM" id="CLU_661590_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="415" mass="46774" checksum="7A9C44714DEA8920" modified="2002-06-01" version="1">MCLVLVEEATTVPTFPGALYVLDPPGEPGRLREGPELTDEEGRSVVPFVALVEPPLREVIRVEGTTLHVHPDAVVHVDPDQPVVLTGYACDDRHTVTAVLPFRSFLVALDHARHGPPDPYELFEVFAILYDPIQAAAFTLNARGHALLIDEHGVNEHAMLYTTPPGHPGPDHISSRHVRPSDLQGAPKHVTGDGPPILPRDRYLDLLARTQLITGVPEPGRADFRLLENVHEYVRPLLTTIHLAHHWETRQVHEETGRLVAEILRGDLHREVADAHKRAVRALGSRYPTPAYAPDARVRWARQLAGPVSDRVLTKVQDFARLVACHRLVNRLWTFVDTVTLLGYEECVDLVEMAYSYTEHDLPGHEIERLLRPWIDALRDPHYRRLERGTAPPPKLRLVEPEEPEDLLVCYDRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF9</accession>
    <name>Q8TWF9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ordered locus">MK1076</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1076"/>
    <dbReference type="EnsemblBacteria" id="AAM02289">
        <property type="protein sequence ID" value="AAM02289"/>
        <property type="gene ID" value="MK1076"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1076"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1130"/>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="OMA" id="HAHGDNM"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="PTHR39648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MAF_flag10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MAF_flag10" evidence="6">
        <location>
            <begin position="49"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="25585" checksum="8BFE90BED8037C89" modified="2002-06-01" version="1">MSDPLKGIIEEIRADLGYKESEDLWAASFLCELLRDHPRVLRPEDLPVEEEVVVVGAGPSIAELPKIVGELEDSVVYAADGACRALLELGIVPDVVVTDLDGPKEYLLMSSECGSITVVHAHGDNVTELAELVPTLGRILGTCQVEPPCDLLHNFGGFTDGDRAVVMAARLGAERVLTVGMDFGNLTTEYSRPGEGRGVFRADPVKRKKLAWGERVLRLVERELGVEVESLTVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG7</accession>
    <name>Q8TWG7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1067</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1067"/>
    <dbReference type="EnsemblBacteria" id="AAM02280">
        <property type="protein sequence ID" value="AAM02280"/>
        <property type="gene ID" value="MK1067"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1067"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1121"/>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_2"/>
    <dbReference type="OMA" id="VMDVFDY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="403" mass="45715" checksum="E37604105D76EE4C" modified="2002-06-01" version="1">MVVDKHARDAIRSGALSVFAPAVRRVRGRFRPGDVVKIETATGDFLGYAFAQTSDRDVRSGRAPGIVARIFDRKGEYPERSPDEIVLERIERRYRFRKRWKVDAHRDHMRVVFSEADDVPGLILDKFNDIAVFQTTCPAIERIILRNAAELLEILDVDTLYEKNDSRKRRQLGMEVRKRVVAGEERVETVVEEYGVRFAVNVEEGQKTGFYIDQVENRAKVQELVDGGRVLDVFTYVGGFAIHAAVGGAEEVVGIDKFDRVVQAAYRNADLNDVRDRVKFLVGDAFALLEKFERRGEEFDVVVLDPPAFVTSKEHLNRGRRAYFDVNYKALGLVRDGGLFVTCSCSHFLEPSDFVRLVNEAAARRSVRLRMLGPLRGQPPCHPIVPGNPDTRYLKAMFCAVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI8</accession>
    <name>Q8TWI8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">O-succinylbenzoate-synthase-related enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02259">
        <property type="protein sequence ID" value="AAM02259"/>
        <property type="gene ID" value="MK1046"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1046"/>
    <dbReference type="HOGENOM" id="CLU_1212609_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="228" mass="24396" checksum="530967659F2820E8" modified="2002-06-01" version="1">MAVMDAEARSQGLPLAELLGGEPRTVESACSVHELGKHGALREARDNLSVGYTVVRVRADSAGLSSVSALDFETVLLEFTDRPSERALEEVRSVSDAEIVVISPEEFDAEVPIYVRVTSEEDIHGLGSAEGVALSVQEVGFLDAVLLGRKARELGFKIIVLTEVESAVSVKAAAHLAGALRAEYCDLSGHLALYEDLESLGYAPEVELTGPGREVRVNHDPYELAEAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ5</accession>
    <name>Y977_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MK0977</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0977</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWQ5"/>
    <dbReference type="SMR" id="Q8TWQ5"/>
    <dbReference type="STRING" id="190192.MK0977"/>
    <dbReference type="CAZy" id="GT1">
        <property type="family name" value="Glycosyltransferase Family 1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02190">
        <property type="protein sequence ID" value="AAM02190"/>
        <property type="gene ID" value="MK0977"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0977"/>
    <dbReference type="HOGENOM" id="CLU_060247_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000215620" description="Uncharacterized glycosyltransferase MK0977">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="344" mass="38220" checksum="12021B960589F9CD" modified="2002-06-01" version="1">MGLGRELRRIGLEPVFSSFGEGREMLMREFPDAPVYGLPKIELFSEDGSFDLLLLLRRHPDLPLRFYAGVEADRRVIRRHGCKVVVSDCQFHALVAAQIIGVPAIVISNMLRVPGEGSLVRLINGMLRRMFELADIVLIPDTYDDTYDVPEIDTEVVWVGPILKRRPDELPPRDAVRRKYGIPDDATVVLVTAGGSKYGRRIVRIAVEGLKLLSKSIDVFPVIISEERVGDGLGLQLRYVDNLLELIKVSNVVITHGGHTTLSECACLRTPVVSVPLPNHPEQHMNAERVLQRGLGVAVPPEELSPKRIAEAIEQAIDWKVPKIRMMDGRGAERAARIVAGTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR3</accession>
    <name>Q8TWR3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted N6-adenine-specific RNA methylase containing THUMP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0969"/>
    <dbReference type="EnsemblBacteria" id="AAM02182">
        <property type="protein sequence ID" value="AAM02182"/>
        <property type="gene ID" value="MK0969"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0969"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1014"/>
    <dbReference type="HOGENOM" id="CLU_032119_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="51"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="352" mass="39227" checksum="F4A674A0CE59134E" modified="2002-06-01" version="1">MTSGVLCTTAPGLEDVCAQELGEITGKSVRENYLGVRGRVLVECSEHEALDLAREINRRSLTVHRAAVLLGGFEIEHRDERGLEEIRERCRELPFERYIHEHDTFGVRPSRLGEHDFTSVDVGAAVGDAVIERIKREEGFRPQVDLDAPSVIVRADVVGDTVIVGVCTTGDRSLHQRGYRVYDHPAALNPVIAQGMLELAGDPDSLIDPTCGGATVPIEALLRDPETEAVGVEKFRTHYEGARLNVLAARVDVELYLADATRLFEEVPELRDREFDAAVFNPPYGLKIANPRVVKTLYRGLARVLSDLVSVVVTVTPRDGWMRAAMEEYGFRLSHDRWVRHGGLDVRLLVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR7</accession>
    <name>Q8TWR7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted ATPase involved in replication control, Cdc46/Mcm family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mcm2_1</name>
        <name evidence="4" type="ordered locus">MK0965</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="DIP" id="DIP-48327N"/>
    <dbReference type="STRING" id="190192.MK0965"/>
    <dbReference type="EnsemblBacteria" id="AAM02178">
        <property type="protein sequence ID" value="AAM02178"/>
        <property type="gene ID" value="MK0965"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0965"/>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_2"/>
    <dbReference type="OMA" id="NAYTCDR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="3">
        <location>
            <begin position="263"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="656" mass="74062" checksum="806FEBA44236E8D4" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT1</accession>
    <name>Q8TWT1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ca2+/Na+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ecm27_2</name>
        <name evidence="7" type="ordered locus">MK0951</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02164">
        <property type="protein sequence ID" value="AAM02164"/>
        <property type="gene ID" value="MK0951"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0951"/>
    <dbReference type="HOGENOM" id="CLU_007948_0_0_2"/>
    <dbReference type="OMA" id="HKDAQFY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00367">
        <property type="entry name" value="TIGR00367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="26"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="189"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="329" mass="34143" checksum="B689AFDB565CE2A1" modified="2002-06-01" version="1">MNGLKPCSVRALPVRGSDRVDPWSALAVMMGCFVGFHFLSELLVDATVGLARKYGLSESVAGATLAAIGTSAPEFGSSLSSILLEHPNVGVGTILGSAVYNVTVIPGLAALAAGGLTLERAVYRRDVLFYLLALVVVLVSLWDRVVLRVEALAWVALYGLYVLLMRRTDESTIGAEGDTGEASLRSLVVRVSVAVVGIAALSDLMVRATVDFCEGFGLSESRVSLLLNAAGTSVPDTLASVHAARRGFGSLAVSNAVGSNTFDLLVCLGVPLSLVSRTPVHGELGATVLALVGCVVLLYLVTVDGKLRNVEALALLGAYAAFVACLLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV4</accession>
    <name>Q8TWV4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02140">
        <property type="protein sequence ID" value="AAM02140"/>
        <property type="gene ID" value="MK0927"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0927"/>
    <dbReference type="HOGENOM" id="CLU_1207616_0_0_2"/>
    <dbReference type="OMA" id="PGHPQSK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="254" mass="28803" checksum="BB56EC7BECC1A111" modified="2002-06-01" version="1">MGLRRLLGLVGVGLIVLYFTLLSAEYSEFQRFLGDPWGRAVYVKLHNPDIAPGTPHIRTLVDFAKQHGYSKILVIHNAGKIGYSHFWYHGILIIQLGWYDYRTVFAQPELGLVMEDILYGQRPMLGIWIADRREYRSMSEAVNHVRDLESAVPGRTMVVWHGSCRNGNPLWNLGCGAEPYFWILSAYGGRVLALTFAVLGNFAPIVFYGDAALAELRNYDKLQALYNSGTLNRYAVDPYLRKKPMPMGPRGVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW5</accession>
    <name>Q8TWW5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02129">
        <property type="protein sequence ID" value="AAM02129"/>
        <property type="gene ID" value="MK0916"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0916"/>
    <dbReference type="HOGENOM" id="CLU_1431653_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="189" mass="20210" checksum="B87486EED5CDF694" modified="2002-06-01" version="1">MLSPETVVTINTIITSLPFLAAIYVIAKRPARALIQLYSLGYLTPVAVLVYGLAIPVPGNLYAYFTGAPIFLAYGVTDALIVLYALGSALAVIFVGIDQWADVAYSASTTVAWLLRTLIDPMSPRLAVLSGALITATINALVAWYKYQAWPIVPLPSVALQFSVAVASAVTLEVTAWWVLNKLKPSPQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX0</accession>
    <name>CARB1_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Carbamoyl-phosphate synthase large chain, N-terminal section</fullName>
            <ecNumber>6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">carB1</name>
        <name type="synonym">carB_2</name>
        <name type="ordered locus">MK0911</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="domain">
        <text>Corresponds to the N-terminal section.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">CarB is split into two genes in M.kandleri (MK0911 and MK1666).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWX0"/>
    <dbReference type="SMR" id="Q8TWX0"/>
    <dbReference type="STRING" id="190192.MK0911"/>
    <dbReference type="PRIDE" id="Q8TWX0"/>
    <dbReference type="EnsemblBacteria" id="AAM02124">
        <property type="protein sequence ID" value="AAM02124"/>
        <property type="gene ID" value="MK0911"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0911"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.953"/>
    <dbReference type="HOGENOM" id="CLU_000513_1_1_2"/>
    <dbReference type="OMA" id="ADRMFYL"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145077" description="Carbamoyl-phosphate synthase large chain, N-terminal section">
        <location>
            <begin position="1"/>
            <end position="564"/>
        </location>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="400"/>
            <end position="561"/>
        </location>
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            <end position="214"/>
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        </ligand>
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        <location>
            <position position="282"/>
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        <ligand>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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            <name>[4Fe-4S] cluster</name>
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</entry>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
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                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
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                <person name="Aravind L."/>
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                <person name="Rogozin I.B."/>
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        </source>
    </evidence>
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        <source>
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</entry>
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        <name evidence="2" type="ordered locus">MK0845</name>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
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                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="30"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="229" mass="25675" checksum="1349E41B52B675EA" modified="2002-06-01" version="1">MELRYRTPSGMSCLYLPRCAFPRSGRDAWRKKKQVETILTLLCSRLKPGDTVVDCGCGRGTLTTALAVSSPSCDVLGFDIKEFETWTELERYVESVGLDNLEFEVNDLHETAKYGLPDDPDVIVGLHLCGTLTDRLLKLAVNSDAHFVLVVPCCYGRASPRVIVEELGVDEGIERVREVLKRAESSVEYQLEVCRMRGKFLEEHGYQVHVGVAFPEEISGRRVFLMGIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX43</accession>
    <name>RL14E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14e</name>
        <name type="synonym">rpl14a</name>
        <name type="ordered locus">MK0833</name>
    </gene>
    <organism>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX43"/>
    <dbReference type="SMR" id="Q8TX43"/>
    <dbReference type="STRING" id="190192.MK0833"/>
    <dbReference type="EnsemblBacteria" id="AAM02046">
        <property type="protein sequence ID" value="AAM02046"/>
        <property type="gene ID" value="MK0833"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0833"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.876"/>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="OMA" id="RRCNIKH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_L14e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_protein_L14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="PTHR11127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132047" description="50S ribosomal protein L14e">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <sequence length="94" mass="10206" checksum="CB4D870D459B0534" modified="2002-06-01" version="1">MPAPIEVGRICVKTAGREAGKYCVVVDIVDENFVIITGPKDVTGVKRRRCNIKHLEPTPEKVDIDRGASDEEVKEALEEAGLLDLMKEGIVSGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX60</accession>
    <name>FTRL_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Formylmethanofuran--tetrahydromethanopterin formyltransferase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0816</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX60"/>
    <dbReference type="SMR" id="Q8TX60"/>
    <dbReference type="STRING" id="190192.MK0816"/>
    <dbReference type="PRIDE" id="Q8TX60"/>
    <dbReference type="EnsemblBacteria" id="AAM02029">
        <property type="protein sequence ID" value="AAM02029"/>
        <property type="gene ID" value="MK0816"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0816"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.858"/>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="OMA" id="CGDGYEW"/>
    <dbReference type="BRENDA" id="2.3.1.101">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138127" description="Formylmethanofuran--tetrahydromethanopterin formyltransferase-like protein">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="300" mass="32966" checksum="EF08FC68601ADC0E" modified="2002-06-01" version="1">MVEINGVPVEDTFCEAFKGLYARFIVTAADERPLREAAENVAALPATVFGESEAGVERWLDPEETPDGRPGFVAQMWVEYGDDAVKKLEHELGKRIRQGVLVRPTTRVFDACEDPDGYIDTERPIGRCADGYEYTDVRFDREMVHVPIMMGEFLIERRLGYAEGVAGGLVWLFCEDVDAALEAGYRAVEALRDVEGVITPFNVCAAGSKPETIYPDIGPTTNHPYCPTLRDRILDSKVPEGVEAIPEIVINGVSLDVVKRAIGIAIEAATEVDGVVKVSAGNFGGKLGDYRIPLRECIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC0</accession>
    <name>Q8TXC0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase</fullName>
            <ecNumber evidence="1">6.3.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl phosphate synthetase</fullName>
            <shortName evidence="1">CPSase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">carB_1</name>
        <name evidence="5" type="ordered locus">MK0754</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of carbamoyl phosphate from ATP, ammonium and bicarbonate. Proceeds via a three-step mechanism, i.e. the phosphorylation of hydrogencarbonate to carboxyphosphate, a nucleophilic attack of ammonia on carboxyphosphate yielding carbamate, and the phosphorylation of carbamate forming carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + hydrogencarbonate + NH4(+) = 2 ADP + carbamoyl phosphate + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small carbamoyl-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0754"/>
    <dbReference type="EnsemblBacteria" id="AAM01968">
        <property type="protein sequence ID" value="AAM01968"/>
        <property type="gene ID" value="MK0754"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0754"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.794"/>
    <dbReference type="HOGENOM" id="CLU_734905_0_0_2"/>
    <dbReference type="OMA" id="YQRITIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02221">
        <property type="entry name" value="CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043673">
        <property type="entry name" value="CPSase_Archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="4">
        <location>
            <begin position="110"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="347"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="379" mass="43362" checksum="084F3D6B49BCD765" modified="2002-06-01" version="1">MKVLFVGARLLRDVAEYAGSTGVYRILTESNPRSRDWELADEVHFVPRGMEHPTRIARERHVDGVVPLIGVDDPLPAVGEMKEELEAEGIRVVASDRESVEIGIDKVRTKRAFEELNVPTPEWEVVENERPSISPPVVVKDPRSQAGLGVTVHETHKPRVSGRKLVEEFIEGAEVSIEVLSWDGEVVPLVPVFKGSTADRRHPIDRMRMSPAPIDPSVERKIRRCAVRVVEHLGLEGNVDFDVVVRENEFWFLEFNPRPSGTRYMTSGCTGIWPLRELVDMAADRWRPPKIRKLWCAIECPVWRPTDKDPLREMFDGGVYHVFYEYYGRFDVGGRVTVRGDSFKEALTRLRSALKAAGADVKRAEREYRRRLREIEEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE7</accession>
    <name>Q8TXE7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0727</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0727"/>
    <dbReference type="EnsemblBacteria" id="AAM01941">
        <property type="protein sequence ID" value="AAM01941"/>
        <property type="gene ID" value="MK0727"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0727"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="86" mass="9438" checksum="E859A1CF4597C7A5" modified="2002-06-01" version="1">MGGSGGEEGGRGAGKTSYEVVIREEHCKGCFLCSWVCPIDALKRSNRRNERGYLLPEWNGECTGCRQCELICPDLAIEVREVEGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG9</accession>
    <name>Q8TXG9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0705</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0705"/>
    <dbReference type="EnsemblBacteria" id="AAM01919">
        <property type="protein sequence ID" value="AAM01919"/>
        <property type="gene ID" value="MK0705"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0705"/>
    <dbReference type="HOGENOM" id="CLU_077875_0_0_2"/>
    <dbReference type="OMA" id="NRMIITC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="113"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35560" checksum="B0A4AABF6EFA6AE8" modified="2002-06-01" version="1">MLRNIGPVIRLIVLILYPLALPLARILVIRMSPEYLRRMDYLLQVSNIDVPYEIYLSASLIYVLIGMSISGPLIPKLGSTAFLLAVMPLYLLIHPRIVQSLRIKDIEENLPDALRQMVEELRAGLSIFETIRNVAESDYGELSREFRIVVRDMDTGKTFEEAILDMAERVNSELLTRAVRLTVRISMSGGALADVLEAVENDIREVRRIELERKAITTMPCLALALGGLISGLPVGVSIGAVIGVAMMERMGPTYAMLPMYLQAKDPLASYPLVLGLLSGMAIGIIRYGNMKRGLVFGLPLGAAAAGVYLAIVSVMPSFLAAGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH2</accession>
    <name>Q8TXH2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted secreted protein specific for M.kandleri, MK-19 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01916">
        <property type="protein sequence ID" value="AAM01916"/>
        <property type="gene ID" value="MK0702"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0702"/>
    <dbReference type="HOGENOM" id="CLU_2857104_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="64" mass="6400" checksum="40DAD0D8C2ACDB6B" modified="2002-06-01" version="1">MALGVRGQGGVEYLLIIAIVAGCAIAVGVIAYYLPISGGKQGLKDVGSAVSGGFERFASSAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK7</accession>
    <name>Q8TXK7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0665"/>
    <dbReference type="EnsemblBacteria" id="AAM01880">
        <property type="protein sequence ID" value="AAM01880"/>
        <property type="gene ID" value="MK0665"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0665"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.703"/>
    <dbReference type="HOGENOM" id="CLU_541447_0_0_2"/>
    <dbReference type="OMA" id="VMARCMN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008303">
        <property type="entry name" value="Methan_mark_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09887">
        <property type="entry name" value="DUF2114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016937">
        <property type="entry name" value="UCP016937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03285">
        <property type="entry name" value="methan_mark_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="478" mass="51454" checksum="F8CD02EC7A501BA0" modified="2002-06-01" version="1">MGLFSKLFRRGPKPQIAESERVTGPLAVSRDYYIVASVELGNTTTKCILTATDLKEGVTYLIHKEVRMTRDVRPPRSGEEVFGKTVWGVELTRDSVAEMVADTLKSAVKNANIKIDDVHFVVRSTGVTAGFASPEEVGEMIKALADGCLKAGVPPNRMTAAMSKEQIPEPFREHSLIDKVYFDGAVTGVKPPAGKEVVANEMEGELVMAGIKVGAKSTKVDFRNPCMAIDYGTTLAGRITNDELPYADTIGNLCGLAGAIMDAIARGCGEIDEEIGCALDLPSDVEPDVGEKARELAEEAHEYIDIREIPANRERFGTVPVNPKAAKKAGLKLLGCDVGENGSDLSRLTEIGREAYEEGGYELLFGVLDWTATLIAKRLIETALDLDLIDENTAIGITGRAGTTGKKPELICRMIAEEFGDYWDPEEDLVFVDDGLARGAATMARCMNCLGTPENPLGGNRGGGCILGLRIKHQQEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK9</accession>
    <name>Q8TXK9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01878">
        <property type="protein sequence ID" value="AAM01878"/>
        <property type="gene ID" value="MK0663"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0663"/>
    <dbReference type="HOGENOM" id="CLU_002017_0_0_2"/>
    <dbReference type="OMA" id="YDVTTHA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="491" mass="55589" checksum="E7575F8ED85A9797" modified="2002-06-01" version="1">MLPTPGVPRLELEEELRKHVPDEAKEFVRSVLSRLLACEREIDRTLEALDGCYVEPGPAGNPTRGDVDVLPTGKNFYSVDPRKVPTRRAWKVGKKLAENTIERYLEEHGRYPESVGIVLWASDVMRTGGEVLSQVMWLLGVRPEWDESGIVSGFEVVPLEELERPRIDVVVRISGMFRDAFPNLVKFLDEVFEAVRELDEPEGWNFVRKHALDHRIFGDPPGAYGAGVNYAVHASAWDDRSDLADVYVRWGGYAYGSDVYGEEAFSEFRRALSTVRAVSVNQSSHEWDVLSCCCHFAFHGGMVAAAEHHSGERVECYHHDTTDPHRVITRPIEEELVRLVEQKLANDEWIEAMLEHGAKGVGEWLKRVENLFGWDATTGRVPSECYEKIEDVLERYGPRFSDENPYAVEYARERLLEAKKTGSVALADDVGRVLLHLRLDADVLVGLDDQRVDHGDDRPDRRRVLDRTEQQVVAAQRRRHYVGAPVGGLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL1</accession>
    <name>Q8TXL1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyl-coenzyme M reductase operon protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mcrC</name>
        <name evidence="4" type="ordered locus">MK0653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 3">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0653"/>
    <dbReference type="EnsemblBacteria" id="AAM01868">
        <property type="protein sequence ID" value="AAM01868"/>
        <property type="gene ID" value="MK0653"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0653"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.692"/>
    <dbReference type="HOGENOM" id="CLU_099719_0_0_2"/>
    <dbReference type="OMA" id="PVCEITF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007687">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003137">
        <property type="entry name" value="McrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03264">
        <property type="entry name" value="met_CoM_red_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="202" mass="22022" checksum="6035E4F551D3D32E" modified="2002-06-01" version="1">MGRETYAVDCRAAMGMGKGGSLAQRGTIAETEITEVVAVAMSPGSRHITKPVCEITYALREAGIHTSVLVLNAGSGVPAEAPVQTGATMGIEPEEIERINRHEVAVIHLGNVKQHIVWKARLILKHCDVPAVIVCQTPVDFEDFAEVGCRTRIVEPPEPETVGEVVEIVTGVIRGETVPSDKINEIVRKVRRALRYARRRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL3</accession>
    <name>Q8TXL3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01864">
        <property type="protein sequence ID" value="AAM01864"/>
        <property type="gene ID" value="MK0649"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0649"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.688"/>
    <dbReference type="HOGENOM" id="CLU_738913_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="374" mass="41942" checksum="BC70296D4BFB1C5D" modified="2002-06-01" version="1">MDHDAVAMLMEVRADLKEVKALYQRLARIIRQAGLTNDYEYIISTAAALLVHDKYEKAREVLSGLKNPREGAMLTMAALREASLLDEILGEHTVEPAELVVNGVGSFDEALKKLDEYLVGEGEVRRQPGPDMSYLRATDGAISNHLLGRTVGYTLNTTPRDYAEFVENSVHAFAYARLLPSHQWRVRLSLEPYVTNPLSPPSLMRTLELVDELRDPELVMSELDVSENTFLRYYKTLVRYLLVHPDHEICEPTELGERALDDEEVLAEIVLKRGFWAILSKHPNGGSVAEKLAKRSVVNRKMKWKDEVDISLLVDAASDTDRAEAELPVVLAVHAEELSTVHLAARAGVSTGVAAEVLYEHFPEQDWEGERFGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM4</accession>
    <name>TSR3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0638</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an N(1)-methylpseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methyl-N(3)[(3S)-3-amino-3-carboxypropyl]pseudouridine in 16S/18S rRNA + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXM4"/>
    <dbReference type="SMR" id="Q8TXM4"/>
    <dbReference type="STRING" id="190192.MK0638"/>
    <dbReference type="EnsemblBacteria" id="AAM01853">
        <property type="protein sequence ID" value="AAM01853"/>
        <property type="gene ID" value="MK0638"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0638"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.678"/>
    <dbReference type="HOGENOM" id="CLU_035060_4_2_2"/>
    <dbReference type="OMA" id="DCSWESA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:18S rRNA aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="PTHR20426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000094419" description="16S rRNA aminocarboxypropyltransferase">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="E1QU22"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="169" mass="19233" checksum="1C33FB1EDF538FE1" modified="2002-06-01" version="1">MIVLHARDCDPKACTALRAHRMGLVELTRHPGDVPTGAVVLDPTVEKALSREDRDAALERGLVAVDCSWEHVHRYFGPLRRRCRHRILPYLIAANPVNYGKPCKLSTVEALAAALYILGFRREAEEFISRFKWGPAFLELNRERLEAYRRAETSAEVVRVQEEFLPDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM6</accession>
    <name>Q8TXM6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01851">
        <property type="protein sequence ID" value="AAM01851"/>
        <property type="gene ID" value="MK0636"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0636"/>
    <dbReference type="HOGENOM" id="CLU_1682726_0_0_2"/>
    <dbReference type="OMA" id="DIGYRFF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009183">
        <property type="entry name" value="UCP004962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09897">
        <property type="entry name" value="DUF2124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004962">
        <property type="entry name" value="UCP004962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="152" mass="17067" checksum="59D2DE5D28C1A2B1" modified="2002-06-01" version="1">MPEKGLKPFLTGFRETVLDLTDGEGVLVYSGCAGTCTPFAELLAFTLRGTDLEQYYSIDLRREYLRMELRDHGYTVTDEREELESADVVVLLGGLAMPISDATPDDAREFLEELGNPPLVGVCFMNMFERAGWTDELDFHTIIDGYLEVTVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN4</accession>
    <name>AROA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">MK0628</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXN4"/>
    <dbReference type="SMR" id="Q8TXN4"/>
    <dbReference type="STRING" id="190192.MK0628"/>
    <dbReference type="EnsemblBacteria" id="AAM01843">
        <property type="protein sequence ID" value="AAM01843"/>
        <property type="gene ID" value="MK0628"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0628"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.667"/>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="UniPathway" id="UPA00053"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088330" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="1">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="171"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <sequence length="428" mass="45827" checksum="CD8C67E906E935BD" modified="2002-06-01" version="1">MKRVELEGIPEVRGTVCPPPSKSGSHRALIAASLCDGSTELWNVLDAEDVRATLRLCRMLGAEVDVDGEERLEATVSGFGDSPRAPEDVVDCGNSGTTLRLGCGLAALVEGTTILTGDDSLRSRPVGDLLAALRSLGVDARGRVVRGEEYPPVVISGRPLRERVAVYGDVSSQFVSALLFLGAGLGALRVDVVGDLRSRPYVDMTVETLERFGVSVVREGSSFEVEGRPRSPGKLRVENDWSSAGYFVALGAIGGEMRIEGVDLDSSHPDRRIVEITREMGAEVRRIDGGIVVRSTGRLEGVEVDLSDSPDLVPTVAAMACFAEGVTRIENVGHLRYKEVDRLRALAAELPKFGVEVREGKDWLEIVGGEPVGARVDSRGDHRMAMALAVVGAFARGKTVVERADAVSISYPRFWEDLASVGVPVHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP0</accession>
    <name>Q8TXP0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA modification enzyme consisting of a 3-phosphoadenosine 5-phosphosulfate sulfotransferase fused to RNA-binding PUA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0621"/>
    <dbReference type="EnsemblBacteria" id="AAM01836">
        <property type="protein sequence ID" value="AAM01836"/>
        <property type="gene ID" value="MK0621"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0621"/>
    <dbReference type="HOGENOM" id="CLU_026622_0_0_2"/>
    <dbReference type="OMA" id="RWCCKVT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="124"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="524" mass="59067" checksum="ECB55EA482772EC0" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT0</accession>
    <name>Q8TXT0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Activator of 2-hydroxyglutaryl-CoA dehydratase (HSP70-class ATPase domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0580"/>
    <dbReference type="EnsemblBacteria" id="AAM01795">
        <property type="protein sequence ID" value="AAM01795"/>
        <property type="gene ID" value="MK0580"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0580"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.615"/>
    <dbReference type="HOGENOM" id="CLU_666680_0_0_2"/>
    <dbReference type="OMA" id="ATCFRCA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017676">
        <property type="entry name" value="Methan_mark_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03286">
        <property type="entry name" value="methan_mark_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="3">
        <location>
            <begin position="149"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="407" mass="43573" checksum="2D81B32E80E9CB4A" modified="2002-06-01" version="1">MAVQIAQLSCGTEYSGVQPEIERAAERVGAEIVLPEVDIDIVEEACEEVGYTPKSANLKVLLARAYALANGYAEADAAIILTCFRCAEGALVRSEARRYLQENTDLPVVTYSFTEDLDASELLTRMEALVTIVERKGLLARKRQEGLTLGIDSGSTTTKAVVMEDDEVIGTGWVRTTKVVESAEKAVERALEEAGYELDDIEAIGVTGYGRYTLGRHFDADLVQEELTVNSKGTVYLGDKQEGGATVIDVGGMDNKAITVWDGIPDNFTMGGVCAGASGRFLEVAADRIGVDLDKFGKLALEGDPEAVRLDSYCIVFGIQDLVTALAEGADPEDAAAAACRSVAEQIYEQQLQEIDIREPVFFVGGTSLVDGMVKALEDVIGVEVVVPEYPQYIGAVGAALLASNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW0</accession>
    <name>Q8TXW0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0549</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0549"/>
    <dbReference type="EnsemblBacteria" id="AAM01764">
        <property type="protein sequence ID" value="AAM01764"/>
        <property type="gene ID" value="MK0549"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0549"/>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="OMA" id="SGEIMGY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:peptide alpha-N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="PTHR23091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="32"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="185" mass="21473" checksum="5C8CF8C9C1EA4C3D" modified="2002-06-01" version="1">MYRITSKKLETRRLNGAVVVTIEYVDKVGRRVVVRRAKRSDIPEVVEIEERAFPKSPYPTYVFLYNLSNNPEGFLVAEVGGKVVGYVIFELRPWLGEGHIVSIAVHPNYRRTGIGTILMGEAERKIAEAGYETVRLEVRESNFPARRFYERLGYREERREHGYYSDGETAIIMVKKLKSPIQYRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW3</accession>
    <name>Q8TXW3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mg-dependent DNase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tatD</name>
        <name evidence="1" type="ordered locus">MK0546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0546"/>
    <dbReference type="EnsemblBacteria" id="AAM01761">
        <property type="protein sequence ID" value="AAM01761"/>
        <property type="gene ID" value="MK0546"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0546"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.581"/>
    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01090">
        <property type="entry name" value="TATD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="29500" checksum="070976C0917E7E20" modified="2002-06-01" version="1">MKKPVDAHCHLCFKYFKGEEDEVVERSRKKVLRVYDCGATPGTARRTLELAERFEGFVFSTIGLHPPRAPRMQQSVIDDVVRIIREHADRIAAIGEIGLDYHYVKEPGERRRMREVFERFLKLAEELDKPVVIHARDAEEHALEVLEDYDVVAMFHCYDGPAELARRIADAGHYVSLSTIHVIRGPKDERTRELLETVPLEAALTETDSPYLSPVRGERNEPRNVWRVIELLARVKGVPVGEVVETTARNALEFYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW7</accession>
    <name>Q8TXW7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator consisting of a V4R domain and a DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0542"/>
    <dbReference type="EnsemblBacteria" id="AAM01757">
        <property type="protein sequence ID" value="AAM01757"/>
        <property type="gene ID" value="MK0542"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0542"/>
    <dbReference type="HOGENOM" id="CLU_1131613_0_0_2"/>
    <dbReference type="OMA" id="ECYGTGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="245" mass="27512" checksum="8CDE5861973C4824" modified="2002-06-01" version="1">MKLVIDVPKEIAKALDSKTRREILKLLEERGSMTISDIARELNLSKSTVHHHLQELRKAGLVDVIKEDRETPLPKRYYGLVRRLVRVSKPKLEDASEYVIKALERGEDLRTSMFLAIAAAYRAVLESLGIDASEVLYELGKEIGKRLAELGSEETVLRKGLEIIAESVEFRQENDRVVVEIEGCHECSDLPYGPACHLEAGIIAGVLSNLRERPYEVREVECCGEGADRCVFVAEPATDEERPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX8</accession>
    <name>Q8TXX8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ilvB_2</name>
        <name evidence="12" type="ordered locus">MK0531</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0531"/>
    <dbReference type="EnsemblBacteria" id="AAM01746">
        <property type="protein sequence ID" value="AAM01746"/>
        <property type="gene ID" value="MK0531"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0531"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.565"/>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_2"/>
    <dbReference type="OMA" id="CFGTSGP"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="6"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="195"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="393"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="565" mass="61205" checksum="BD7DAF5A59EF8A69" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY04</accession>
    <name>Q8TY04_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0503"/>
    <dbReference type="EnsemblBacteria" id="AAM01718">
        <property type="protein sequence ID" value="AAM01718"/>
        <property type="gene ID" value="MK0503"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0503"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.534"/>
    <dbReference type="HOGENOM" id="CLU_822861_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039866">
        <property type="entry name" value="CPQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053">
        <property type="entry name" value="PTHR12053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M28" evidence="7">
        <location>
            <begin position="91"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="337" mass="36545" checksum="40DD3ED493CD2D7E" modified="2002-06-01" version="1">MNGKILVVTGVAVCLVLATGVVAFNPANKVKEFDPKHALNFAKNICKLGPRYGGNEAELKAANIMEAELKKYGLNVHEEKVDLGGGKYTYNVIGEIKGTDESNKYVIVGSHIDSPGFCEGATDDAAAMGIQVEMARVLAKNFRPKKTVLIIGFGGEELWFKGSEAFVRKHPKIIKNCEAVIDLNCVGAGQNVFLTQKSAKPKPVEGDPKLIKLLEECAKELGHPVTVGDTTYPSDTYPFYHNEIKRVPVCQVMSQPFEVPPWSESNTADKLDPKDMEKVGETVTLAVVKLTNAEPASKPLWEAKRAEESAGGGWPAPMWWAPALLGVAAALLRSRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY06</accession>
    <name>Q8TY06_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized FAD-dependent dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0501"/>
    <dbReference type="EnsemblBacteria" id="AAM01716">
        <property type="protein sequence ID" value="AAM01716"/>
        <property type="gene ID" value="MK0501"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0501"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.532"/>
    <dbReference type="HOGENOM" id="CLU_046973_1_0_2"/>
    <dbReference type="OMA" id="SVYSFCM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028348">
        <property type="entry name" value="FAD-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038984">
        <property type="entry name" value="FAD_binding_protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIDA" evidence="1">
        <location>
            <begin position="355"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="448" mass="48911" checksum="617A08782DDEC5CE" modified="2002-06-01" version="1">MERVVIVGAGPAGLFAAREVALRAERDVEVVVYEQGPSLEDRLESGEVMRGVGGAGGLSDGVLNLRPDVGGDLVSIVGDKRSANELVEYVDEVFLKHGAPKELKVPEGPKVEEIALRAAAADVEFVRIPQRHIGSDNLPKVIGSLVRELEELGVKIVPETRVERILADECVRGVKLEDGREVEADYVIAAPGRVGANWMMHEAKRLGLGLRYNPIDVGVRVEVPRIVMEPVVEVSLDPKFRTRAPTYDDPVRTFCVCHGGYVVKENYEGFVGVNGHSYRRKKSENTNFAFLVSVDLTEPVENTIEYGRSIGRLATTIGGNRPIIQRLGDLRRGRRSTWDRINRSHVTPTLMDVTPGDISMALPHRVTVDIIEGLERLDEVIPGVASDSTLLYAPEIKFYSARVETNEDLETRVENLFVAGDGAGLSRDIVNAAATGVIAGRAVAERIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY52</accession>
    <name>Q8TY52_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0454"/>
    <dbReference type="EnsemblBacteria" id="AAM01669">
        <property type="protein sequence ID" value="AAM01669"/>
        <property type="gene ID" value="MK0454"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0454"/>
    <dbReference type="HOGENOM" id="CLU_145818_0_0_2"/>
    <dbReference type="OMA" id="YGGMKIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="130" mass="14983" checksum="70FA386EFBDF5B7A" modified="2002-06-01" version="1">MFVVIRSDSYEKILTSLSDIERYAGIKILGKPRIMDPEVADTIVRELLGEVRRRYPVAAVARVEGEPAEVIRKISEIHPPAHLIVITPRHGDVYRGIARRFGKLEELRGYHSPKRRIEDDREKEGRETAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY57</accession>
    <name>Q8TY57_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted transcription factor, homolog of eukaryotic MBF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0449</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0449"/>
    <dbReference type="EnsemblBacteria" id="AAM01664">
        <property type="protein sequence ID" value="AAM01664"/>
        <property type="gene ID" value="MK0449"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0449"/>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="OMA" id="VQCEMCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="CHP00270"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00270">
        <property type="entry name" value="TIGR00270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="19804" checksum="1D8F4A2F121732C0" modified="2002-06-01" version="1">MEERIRCEICGRVINGRPKVVKVEGSELRVCEECAKFGREVVKPRPRRETGRVQRERRPRRRPTGARRRPRGFDPFSEGLEVVPDYDERVREARERRGWSQEDLAKKIGEKVSVIRRIESGKMEPDVELARKLERVLEIELLERVSEEDTGSVGIGSGELTLGDVVEIRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY86</accession>
    <name>IF2A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2a</name>
        <name type="ordered locus">MK0419</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY86"/>
    <dbReference type="SMR" id="Q8TY86"/>
    <dbReference type="STRING" id="190192.MK0419"/>
    <dbReference type="EnsemblBacteria" id="AAM01634">
        <property type="protein sequence ID" value="AAM01634"/>
        <property type="gene ID" value="MK0419"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0419"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.448"/>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="PTHR10602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="SSF110993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="SSF116742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000137395" description="Translation initiation factor 2 subunit alpha">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <sequence length="267" mass="30400" checksum="EED920127E79120E" modified="2002-06-01" version="1">MPRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKEGQKVVAKVIRVNPKRKYANLSLRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDEAYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEEWAEKLAELAIENIEPGRVKIEAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMVRKAAQAAIDHVEEHGGEGEFHREIEEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD7</accession>
    <name>GUAAB_METKA</name>
    <protein>
        <recommendedName>
            <fullName>GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber>6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">guaAB</name>
        <name type="synonym">guaA_1</name>
        <name type="ordered locus">MK0363</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYD7"/>
    <dbReference type="SMR" id="Q8TYD7"/>
    <dbReference type="STRING" id="190192.MK0363"/>
    <dbReference type="EnsemblBacteria" id="AAM01578">
        <property type="protein sequence ID" value="AAM01578"/>
        <property type="gene ID" value="MK0363"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0363"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.385"/>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="OMA" id="EGGIKSH"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140242" description="GMP synthase [glutamine-hydrolyzing] subunit B">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="314" mass="35327" checksum="B37326D571706311" modified="2002-06-01" version="1">MFDPKKFVEEAIEELRREIGDRKAIIAVSGGVDSTTAAVLTHRAIGSHLVCVFVDHGFMRKGEPERIRELLEEELGLNLRFVEAAEEFFEALRGVTDPEEKRKIIGEKFIEVFERIAEEEEAEVLVQGTIAPDIIESERGIKSHHNVGGLPEKLNLDVVEPLRDLYKDEVREVARYLGIPDEIVERMPFPGPGLAVRVLGEVTPEKVEIVREANAIVEEEVEKAVEEGKMSKPWQAFAALLDCKATGVKGDERDYGWVIAVRIVESIDAMIADVPEVPWEVLRNIQDRITSEVPEVTRVLFDITPKPPATIEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE6</accession>
    <name>Q8TYE6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0354</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01569">
        <property type="protein sequence ID" value="AAM01569"/>
        <property type="gene ID" value="MK0354"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0354"/>
    <dbReference type="HOGENOM" id="CLU_2204131_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="107" mass="12615" checksum="57D27DE1A88B9D8B" modified="2002-06-01" version="1">MIMEILNRHKEKLVKLRVLSYGDRMILSAQFSANDFDEAYEMLQEIMEEEPEVVSLDSDRKQGYAVTLMEDVTEEYQEIRERVHELMRELVEGGEGKEEFPLPIFEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE8</accession>
    <name>Q8TYE8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01567">
        <property type="protein sequence ID" value="AAM01567"/>
        <property type="gene ID" value="MK0352"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0352"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.373"/>
    <dbReference type="HOGENOM" id="CLU_1623472_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="163" mass="18636" checksum="E1469E9F87BB2DC4" modified="2002-06-01" version="1">MAETIVRKFDPNKWVLVPTRHALERLRKRELSPSADVPEAVHALRRLASTTKVLIKNDVWVAVGTERTLVLSEMRTMSLEKYQDELKRHLSRLHPTYTVYVITAEGCRPTSAGQLDVDDLATEFEYARFSGEARTLVLAREGEKALAVVTVRPPRKKERKLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH6</accession>
    <name>Q8TYH6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable formylmethanofuran dehydrogenase subunit F, ferredoxin containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwd_F2</name>
        <name evidence="2" type="ordered locus">MK0323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0323"/>
    <dbReference type="EnsemblBacteria" id="AAM01538">
        <property type="protein sequence ID" value="AAM01538"/>
        <property type="gene ID" value="MK0323"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0323"/>
    <dbReference type="HOGENOM" id="CLU_1736429_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="150" mass="17210" checksum="6557089514B08587" modified="2002-06-01" version="1">MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTERGVPKLPKNVEVDEDRCVYCGVCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPCNALEFGRDGTAEKTRIVVNLDRVLGPTEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL3</accession>
    <name>Q8TYL3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01500">
        <property type="protein sequence ID" value="AAM01500"/>
        <property type="gene ID" value="MK0283"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0283"/>
    <dbReference type="HOGENOM" id="CLU_2127835_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="113" mass="13273" checksum="850FDC395F6704BD" modified="2002-06-01" version="1">MIEIMTREEFFDLITEGLKTAEIRPSDHRSFRYLEPGDTLVFKNFKAGTMRCIETVVRNVEKDLDPKEAAERFYQEAGFESPEECLEGLKEMYDGLPEKVDVAEFEPVREWEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL4</accession>
    <name>PIMT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="1">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcm</name>
        <name type="ordered locus">MK0282</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYL4"/>
    <dbReference type="SMR" id="Q8TYL4"/>
    <dbReference type="STRING" id="190192.MK0282"/>
    <dbReference type="EnsemblBacteria" id="AAM01499">
        <property type="protein sequence ID" value="AAM01499"/>
        <property type="gene ID" value="MK0282"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0282"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.285"/>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="OMA" id="TISAIHM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000111917" description="Protein-L-isoaspartate O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <sequence length="226" mass="25207" checksum="1BCD87E3265E61B4" modified="2002-06-01" version="1">MAEDDKVFRRARERLVERLKSLGYIRSNRVAEAMLKVPRHEFVPEDLRDRAYVDSPLPIGRGQTISAPHMVAIMTELLDPRPGHKVLEVGAGSGYHAAVVAELVKPDGRVITVERIPELADFARNNLKKTGYDRFVKVLVGDGTKGYPPEAPYDRILVTAGAPDVPESLLEQLKPGGKMVIPVGDRHMQELWLVEKTEDGEIRRRRHGGCAFVPLIGEEGFQEPES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL5</accession>
    <name>RISB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="1">DMRL synthase</shortName>
            <shortName evidence="1">LS</shortName>
            <shortName evidence="1">Lumazine synthase</shortName>
            <ecNumber evidence="1">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribH</name>
        <name type="ordered locus">MK0281</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYL5"/>
    <dbReference type="SMR" id="Q8TYL5"/>
    <dbReference type="STRING" id="190192.MK0281"/>
    <dbReference type="EnsemblBacteria" id="AAM01498">
        <property type="protein sequence ID" value="AAM01498"/>
        <property type="gene ID" value="MK0281"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0281"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.284"/>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="OMA" id="SGPGMTR"/>
    <dbReference type="BRENDA" id="2.5.1.78">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134844" description="6,7-dimethyl-8-ribityllumazine synthase">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <sequence length="141" mass="15697" checksum="AB6E47CB7DA0C2CB" modified="2002-06-01" version="1">MSEIRLGLVVTEFNREITYAMEELAVQHAKDLGAKVVERVLVPGSFEVPLAVRKLLERDDIDAVVTLGAIIKGDTDHDQAIAQQAFRKIQDLMVEYGKPVALGISGPGMTRMEALERVHYAKRAVEAAVKMVRRLRELEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM7</accession>
    <name>Q8TYM7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01486">
        <property type="protein sequence ID" value="AAM01486"/>
        <property type="gene ID" value="MK0269"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0269"/>
    <dbReference type="HOGENOM" id="CLU_574424_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="475" mass="51404" checksum="3FEE001DA6D53C70" modified="2002-06-01" version="1">MKALEQLSSYVAENRAGNRWDFKLAGIRVGRGRTVTFFRHILLASLACLTLPIAATCVNEEFESKVESLIQNPDFQGTKEITLYASGSYVMTGRIKVSYRGPEEVEPVEVDKLINGLKELREDLDPLAGQNEPIKDLLGALKKFDDTSDVLEKLKEKTQGLKLAACEIDVDLWYVGETGTPPSDLPQILQQLRTEGELHLSYKIPYPEAHVYVVGSVELLDRALNLPIRAVFSYDYPNDGSGVKVSVDTEHRKFTIETTDNIYNLIVVELTLHGGLQEVEKLADLSVESSEDLQKLLENTEVKVECYPRGGRTAEINGLPLMVDEGAAIVVYAVGSNPINSIVASKIAELMGKNPLVVTLGNADLQVGTDGKTLVIKGSAPGGLVVIYGPAGPDNTLDVTSRVGTLQVARALGAQVPPLVSLFGWILGPLLGSITTTYTPAFSGELRMSPRGIGMATPAVIAALLLGVHGRRRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN0</accession>
    <name>Q8TYN0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420-reducing hydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0266</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0266"/>
    <dbReference type="EnsemblBacteria" id="AAM01483">
        <property type="protein sequence ID" value="AAM01483"/>
        <property type="gene ID" value="MK0266"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0266"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.269"/>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="VQNNPAM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="482" mass="54092" checksum="6E35EA98D2FE371B" modified="2002-06-01" version="1">MRGVGTLSEMVVNVEPVTRVEGHGKIVVEFDEDGNLKDARFHVVEVRGFEKFLEGRPIEDAPILTPRICGICQVAHHLASAKAADEVFGVEPPEPARKLRELMHHAATVHSHALHFYFLAAPDLVKPEEEDPMKRHVLALARENPEVVKCAIELRKIGQTIVEAVGGKPIHPVTAVPGGVSKPLEEDRREELLELARSAVELAERTVDLAEELTRRLEEEGLLELGYLESYHMGMVSDGVHELYDGVIRVVDPEGNVDREFEPSEYLDHIAEAVRPYSYLKFPYLRDKGEEEGLYRVNTLSRLNVCDRMATPRAQGRYEELVDEYGKPCHHPMLYHYARTIELLSSAERCVELLEDDEITGDDVREEVDPDDVTGEGIGCVEAPRGTLIHHFKTDEEGLITEVNLIVATVQNNPAMDLGVKKVAEEYLRSPEDASPEVLNRMEMVIRAYDPCLSCATHVLGERPRLTLEVHRAGRLVRIVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP5</accession>
    <name>Q8TYP5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0248"/>
    <dbReference type="EnsemblBacteria" id="AAM01465">
        <property type="protein sequence ID" value="AAM01465"/>
        <property type="gene ID" value="MK0248"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0248"/>
    <dbReference type="HOGENOM" id="CLU_023194_10_0_2"/>
    <dbReference type="OMA" id="VPDMTRW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GFO_IDH_MocA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="GFO_IDH_MocA_C" evidence="2">
        <location>
            <begin position="133"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="317" mass="35722" checksum="545E0B069A57F362" modified="2002-06-01" version="1">MTVRAGVIGVGMMGSHHARVYHELEETELVAVCDVNERRVKEVAKKYDVNWYTDHRKMLQEEDLDGVSICVPTKYHADIAVDALEAGVHVLVEKPIADTIENARRIIDAAEDHGLKLAVGHIERFNPSVMKAKEVVSRGDLGDIVVMSAKRVGPYPPRIRDVGVIVDLAVHDIDVMRYLAETEVEEVYAAAGSAITRTQEDYAEVMLRFEGDPTGLIEVNWLTPHKERRLEVTGREAILEIQYIEQELRLMDREGVKRFNIRKEEPLKLELRDFAESILENRDPLVDGEAGLQALRTAVAALKSVKEDRPVSLEEVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP7</accession>
    <name>RS10_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps10</name>
        <name type="ordered locus">MK0246</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYP7"/>
    <dbReference type="SMR" id="Q8TYP7"/>
    <dbReference type="STRING" id="190192.MK0246"/>
    <dbReference type="EnsemblBacteria" id="AAM01463">
        <property type="protein sequence ID" value="AAM01463"/>
        <property type="gene ID" value="MK0246"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0246"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.248"/>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_S10_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146647" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="102" mass="12009" checksum="DBE195942902AEC5" modified="2002-06-01" version="1">MAQEVRLRLSSTDHYKLEEVCERIKKVVEETGAQMSGPIPLPTKRLLVPTRKSPDGEGKATWDKWEMRIHKRLIDIKGDERTIRRLMRIHIPEEVHVEIIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ3</accession>
    <name>Q8TYQ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0239</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01456">
        <property type="protein sequence ID" value="AAM01456"/>
        <property type="gene ID" value="MK0239"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0239"/>
    <dbReference type="HOGENOM" id="CLU_1773193_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="16890" checksum="EBCE2C9BBFB6D080" modified="2002-06-01" version="1">MTRSYLVFDVGRYRELAEELVKPEDIVVEVGAAAGDTTVRLARNARLVIAFEKSEEMFERLRERVRDLDNVIVLCEDGFELGEVLKRTERVDAVFIDVGGGAQPRLALALWEAYYSRFRPRVIVVRNRRLCRLIETVERVECDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT0</accession>
    <name>Q8TYT0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01429">
        <property type="protein sequence ID" value="AAM01429"/>
        <property type="gene ID" value="MK0212"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0212"/>
    <dbReference type="HOGENOM" id="CLU_955147_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018663">
        <property type="entry name" value="DUF2101_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09874">
        <property type="entry name" value="DUF2101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="291" mass="32099" checksum="8659830F5764338A" modified="2002-06-01" version="1">MVLERIGRFVWKFTAAVGYVTVNLPWIVVKLLKEGPKRSAKFTSQLIRTAEESASKFQTVMEMVREGQVLLPELPQVPFKDIEIRGKRIAAAPRMPWDVPVLVLQFSLLATVVFVILEEIVAPPVMLWEALAVISAFVTAFSLGWLRRVSPSEDIHWLPYWHSTLIILGSVVLSAILTNVPPVVALVLPLEGVLPGFIVFGLPALGVAAATVWVRRKFHRTWTFGVVLREVQEGGSVEVVVGHDIAANTLPGEYIVEGSGSEGTPVLVEVEHSGFSLTGARPVRILKEGWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT3</accession>
    <name>Q8TYT3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01426">
        <property type="protein sequence ID" value="AAM01426"/>
        <property type="gene ID" value="MK0209"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0209"/>
    <dbReference type="HOGENOM" id="CLU_2420055_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="91" mass="10761" checksum="2679E9AA03F7F2F3" modified="2002-06-01" version="1">MYRDTAAFLVEGLDEYLEDMEPSDVAEAVLKAYFEDVERVEEDDTIIFECRTSFPMLRFIHDYAEEHPEFEKYVKPEGDIEKRSEVLEGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU2</accession>
    <name>Q8TYU2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01416">
        <property type="protein sequence ID" value="AAM01416"/>
        <property type="gene ID" value="MK0199"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0199"/>
    <dbReference type="HOGENOM" id="CLU_920131_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="302" mass="35059" checksum="121819CD4715970E" modified="2002-06-01" version="1">MRTVGYNPSLPHGECFKLIRGRKESRETAFGVVDVFKEAFIKWSEGWARLKEFDREEMLSAYSSFFRSRFGVNFKERNVVELLNLIYRIAVEYRNILDVGEKTLTGKLRTYFIPVIMVIMVKQFYGRIKRKEAMKYVRDALQFYKTAYELSGREEGFEEILVGSLLPIEVRKGEKLPKFFEESFKDSTIRKFLGLESESGSVKFGLGCVFIPSWQFRELRAEIKFGKGFGRDGGYVEYVVHRAGTFRECLLMVPQLRLVLVENADTPDKRVFLPFDMLELVPPDPAARMKLESLAVKLTRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW9</accession>
    <name>Q8TYW9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01389">
        <property type="protein sequence ID" value="AAM01389"/>
        <property type="gene ID" value="MK0172"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0172"/>
    <dbReference type="HOGENOM" id="CLU_1286356_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="214" mass="24133" checksum="DAA3398A54F01981" modified="2002-06-01" version="1">MLRVLLIYPRSLRYFVIRAYRELPSTVKPDSPVLLASCDAVRATYTRLEGNVVTVEIGAARNFQEVKASLAHELAHVLQFEHGVMPIGGLPPLRALALPLEVGAEELVLQELPDVAVRRLEMGLRRVTGCRTDDPLSELERSLVLEPLRLAAERLDVPLYVPEVEPEHPEVAKVKKRLLRRVRRIDPWSPLEVQRYLEDAWAEVTVLATLSRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY0</accession>
    <name>Q8TYY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphatidylserine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pssA</name>
        <name evidence="5" type="ordered locus">MK0161</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0161"/>
    <dbReference type="EnsemblBacteria" id="AAM01378">
        <property type="protein sequence ID" value="AAM01378"/>
        <property type="gene ID" value="MK0161"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0161"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.161"/>
    <dbReference type="HOGENOM" id="CLU_049944_3_1_2"/>
    <dbReference type="OMA" id="TIAAHIF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="26080" checksum="6084A9062A1C9E75" modified="2002-06-01" version="1">MERWKISELITPACVVSLTNVVFGAFSLLASIAGFPGAAARFIILSFIADSLDGFVARRTGKESEFGMNLDSLADLVSFAVAPAVLVVTAGLVPAPMCYLLAVLMVCCGALRLARFNAMCVDGYDPGEYYLGLPVPWVGTIASSLYFLTVDLGPSYLWYVLNTVILGTSALLMISSIKFPSLKRPHPAILAAGGLSSLVLLFSFLIPPDEIKRGLEVVASVTITALLSWYMVRGVRGCFRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ3</accession>
    <name>Q8TYZ3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">SelB translation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0148"/>
    <dbReference type="EnsemblBacteria" id="AAM01365">
        <property type="protein sequence ID" value="AAM01365"/>
        <property type="gene ID" value="MK0148"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0148"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.148"/>
    <dbReference type="HOGENOM" id="CLU_019148_1_0_2"/>
    <dbReference type="OMA" id="FGHIDHG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="L35A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004535">
        <property type="entry name" value="Transl_elong_SelB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00475">
        <property type="entry name" value="selB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="459" mass="50006" checksum="EB2DC0E09359C5EC" modified="2002-06-01" version="1">MPVVHVGLFGHIDHGKTALAAQLTEKPSTAALDKHPEEKERGITIDLGFSSFELGDYTVTLVDAPGHADLIRTVVAGAEIIDAAILVVAADEGPQVQTGEHLVVLNHLGIDRGVIALNKVDLVDEKTVERRIEEIKRVLQGTTLEDAPIIPVSAKIGEGIEDLKDALLEVLEPPNRDLDSPFRMPIDHAFHVKGAGTVVTGTVLTGRVEVGDELTLYPIGKTVEVKSIQSFGKDKQEACAGDRVGIALRGIREEEIERGFQLAEEGSLRVTRYLDLKVEIDPLFPQSIGQKTMLHIHVGMRSVPARIVPHDDGFLLDSLRPGESSYLYAKLNEPVAVREGDRTILVKLDLPPTTLRIAGSGLVEDTSKRETFKRVSRRRGRVTRADHMGKGLAVVDGLALNKEHAERLVGEKVRTEGGVEGKIVDTHGTRGAVLVDFEGEVKTGERVVLERVRDVKIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ17</accession>
    <name>Q8TZ17_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01341">
        <property type="protein sequence ID" value="AAM01341"/>
        <property type="gene ID" value="MK0124"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0124"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.123"/>
    <dbReference type="HOGENOM" id="CLU_111491_0_0_2"/>
    <dbReference type="OMA" id="DLCIDNR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019224">
        <property type="entry name" value="DUF2148"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09918">
        <property type="entry name" value="DUF2148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2148" evidence="1">
        <location>
            <begin position="110"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="18913" checksum="89D424C539917AFC" modified="2002-06-01" version="1">MRPESEGVRQAARLMAVAARTAPKTRGIDDIVIEIVEDEDTLEKIAERMEEIAEEKGADFFKRDAECLRRSECLVLIGVKSSGPCGLNCGMCGASCDDIEERSADVEFAGPICGFKLIDLGIALGSAAKVANDLVVDNRLMYTIGVAARSLGVVDADVVIGIPLSATGKNIYFDREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ34</accession>
    <name>Q8TZ34_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diverged homolog of ATP-dependent DNA ligase (Eukaryotic ligase III)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01322">
        <property type="protein sequence ID" value="AAM01322"/>
        <property type="gene ID" value="MK0105"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0105"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.104"/>
    <dbReference type="HOGENOM" id="CLU_777579_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_ligase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="41556" checksum="93FF396C480B3991" modified="2002-06-01" version="1">MREEAPPKIPEGLRITATRTMAAARPEEPEPTLRLFRAFSPDRELVLEEKLDGTNVRVYLEGDRLLAHTRGWVDADEYLRGLDIEPPWEDLRGEFDRITILEGELLPYDLFSQESPRLHELAGRLETEVLFDGEPSDLTAELVYLEGKRYECRPEPLFSRVCELDPDHELIQRFLRENQVKPDFESAVAEGKLEPRVCFYELDMLEGSVKITAPRTEQLRECRRLSTEPPRWRVVVDPDPGDLERELDRLERNWCEGLCVKPMVETDDKLHAVKLRCPWFLRREFDGRPPRRGSHKVAGRITAQLLQTKVLHKNLRELEGKRGVLDKKWRKLSERTQRALRVQDRLLSLGLDSPRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ38</accession>
    <name>KTHY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable thymidylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmk</name>
        <name type="ordered locus">MK0101</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ38"/>
    <dbReference type="SMR" id="Q8TZ38"/>
    <dbReference type="STRING" id="190192.MK0101"/>
    <dbReference type="EnsemblBacteria" id="AAM01318">
        <property type="protein sequence ID" value="AAM01318"/>
        <property type="gene ID" value="MK0101"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0101"/>
    <dbReference type="HOGENOM" id="CLU_1399757_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155387" description="Probable thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <sequence length="194" mass="22113" checksum="6F9539FBF7849734" modified="2002-06-01" version="1">MGLYLGVEGIDGVGKSSVVNLAAEFLEIHGLEVTTVREPSTDIGREALEWDDPYLQALAFTLDRMLTLKRVDFEAADVVLSDRTFLSTLAYQSALGADMRWLLELQRPVPKPDVVYIIDREPLAEDATFDKEFLERVRNRYREAARLIEEEFDVEIKWIEAEDMDKEEIAELIVADARRRLDDPLGIPDDLLEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ52</accession>
    <name>PURO_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMP cyclohydrolase</fullName>
            <ecNumber evidence="1">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purO</name>
        <name type="ordered locus">MK0087</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ52"/>
    <dbReference type="SMR" id="Q8TZ52"/>
    <dbReference type="STRING" id="190192.MK0087"/>
    <dbReference type="EnsemblBacteria" id="AAM01304">
        <property type="protein sequence ID" value="AAM01304"/>
        <property type="gene ID" value="MK0087"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0087"/>
    <dbReference type="HOGENOM" id="CLU_1352116_0_0_2"/>
    <dbReference type="OMA" id="HVDPIAE"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="SSF75569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145796" description="IMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <sequence length="198" mass="22289" checksum="8876A09F18FB18C0" modified="2002-06-01" version="1">MYVGRFLLAGKLEDGTPIAVYGVCSRSFSDRRIEVREGAAFVVPEDPSYITENPYVTYTCARIVDEFLVLTNGAQTDPIADKLESGVPPREALVSVTFAMDYEHDEYNTPRISLITDGETFWLGRVAPEEVYFRVMKPKDGEGYLLSVYGEYAEVPSKPNMTLDREDPLECDPVPSFEHYVCSVIARHDGGRWSLEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ65</accession>
    <name>Q8TZ65_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-dependent hydrolase of the urease superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0074"/>
    <dbReference type="EnsemblBacteria" id="AAM01291">
        <property type="protein sequence ID" value="AAM01291"/>
        <property type="gene ID" value="MK0074"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0074"/>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="OMA" id="CAVQLHT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="PTHR42206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="289" mass="33011" checksum="9F0D24197158A014" modified="2002-06-01" version="1">MMIRGDRLQVATDVHLHFNPVKGDGYKVFEKFHRAGGTGFVSPVLTLRSYRIHGYGREDFERAYRLHLEGVRYGRLEYPLRGYAALGWHPAEVATLAEEHPEEEVLNVAETVCDLIEKFAAEYEEVVAVGEVGHPHYPVPAEVKRVCHKVFVRFLELAKDLDLPVIYHGPKASRKHYMRLYEYLKDVGFDFDRFVRHRATPDVSAARNVGIWPSVPASRRSVREAAEHGPEFMLESDYLDDPRRPNAALPLRAVPKAARILRTIDPDLVSEVMIEIPERVFGVEFEPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ83</accession>
    <name>Q8TZ83_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0056"/>
    <dbReference type="EnsemblBacteria" id="AAM01273">
        <property type="protein sequence ID" value="AAM01273"/>
        <property type="gene ID" value="MK0056"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0056"/>
    <dbReference type="HOGENOM" id="CLU_108786_0_0_2"/>
    <dbReference type="OMA" id="RAAPICY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007501">
        <property type="entry name" value="DUF531"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04407">
        <property type="entry name" value="DUF531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006006">
        <property type="entry name" value="UCP006006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="187" mass="20677" checksum="79EB2FAE40667BE0" modified="2002-06-01" version="1">MPKRAGRVTIGLYNSYDPRRFHEIHARTIARAAPLCVAFDFKLALFGFPLDDLGVETPHELAEYVAEETTIGASGREVLVLAERNLLEVYDYPVRGFPPQLGTIVGTTCRPDERKAIEPEDVVREILRPRSVTLVFGLGRRGLPAEVLEACEYHLDITGRRISLETATAIGAVTAVIGHLIKKELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ91</accession>
    <name>Q8TZ91_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0046</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01263">
        <property type="protein sequence ID" value="AAM01263"/>
        <property type="gene ID" value="MK0046"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0046"/>
    <dbReference type="HOGENOM" id="CLU_1514640_0_0_2"/>
    <dbReference type="OMA" id="GGYHPDT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FtsJ" evidence="1">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="181" mass="20448" checksum="C966E00D1A7DF445" modified="2002-06-01" version="1">MIALVKSVEEYRRLLGEVVEEGDVVVELGCHRGAATRIILTGSPRRVVAVDYGKDAEEAMRELERSHPELTFVKGDAREYDTLKRVLEELGGPECDVLAVDLGGGMFPDTAFKVYYVWSVTLRPRDAVVRNAGLCEFLKLAELREEVHLDDENRGYLGELSPPGIPGRIRERFEEFKLWRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB0</accession>
    <name>KADA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">adkA</name>
        <name type="ordered locus">MK0025</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZB0"/>
    <dbReference type="SMR" id="Q8TZB0"/>
    <dbReference type="STRING" id="190192.MK0025"/>
    <dbReference type="EnsemblBacteria" id="AAM01242">
        <property type="protein sequence ID" value="AAM01242"/>
        <property type="gene ID" value="MK0025"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0025"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.25"/>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="OMA" id="HRDEMRK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000131815" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00234"/>
        </source>
    </evidence>
    <sequence length="191" mass="21692" checksum="3A34413D4073D1EA" modified="2002-06-01" version="1">MGYVIVATGVPGVGATTVTTEAVKELEGYEHVNYGDVMLEIAKEEGLVEHRDEIRKLPAEKQREIQRLAARRIAKMAEEKEGIIVDTHCTIKTPAGYLPGLPIWVLEELQPDVIVLIEADPDEIMMRRVKDSEERQRDYDRAHEIEEHQKMNRMAAMAYAALTGATVKIIENHDDRLEEAVREFVETVRSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB2</accession>
    <name>RL34_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L34e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl34e</name>
        <name type="ordered locus">MK0023</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZB2"/>
    <dbReference type="SMR" id="Q8TZB2"/>
    <dbReference type="STRING" id="190192.MK0023"/>
    <dbReference type="EnsemblBacteria" id="AAM01240">
        <property type="protein sequence ID" value="AAM01240"/>
        <property type="gene ID" value="MK0023"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0023"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.23"/>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="OMA" id="SWRRKYV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_L34Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_L34e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="PTHR10759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131849" description="50S ribosomal protein L34e">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <sequence length="113" mass="13368" checksum="ECE123541A7F3584" modified="2002-06-01" version="1">MPAPRYRSRSCRRVYKRTPGGRTVIHFEKKIPNWPKCGACGRRLNGVMRGRNVELKNAPKTQKRPNRPYGGVLCPECARKLIKDKVRYKFWERKREQPWLPVLPDEEPPEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB6</accession>
    <name>Q8TZB6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protease related to collagenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01236">
        <property type="protein sequence ID" value="AAM01236"/>
        <property type="gene ID" value="MK0019"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0019"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.18"/>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="OMA" id="YHCPFKL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="340" mass="37848" checksum="89E023EB153516EE" modified="2002-06-01" version="1">MFSVPSPGDPALTDRLLRSLPTDRIETVYFGLPIAGTGRATPVQVDTDTATELAEVCHSYSVEPEAVINPLCTADVVCSRNAFAEFERTLDDLDDAGIERLVLSDPLMIHAAVERGFRVSVSCVVEVNTPERARYFDEIGVEEITLDTNVNRRLDTIEAIASEVSARLRIIVNEGCLPDCPYRASHFCLFSHATRPGEEVAEDPYFVRCISERVNNPTLIIKSPFVRPEDLSVYMDLGVRAFKIAGRANSITWIRRAVRAYLRGRYDGNLLDILDCPTVLRHLYHVDNRELDGFLKRVGRCDRRCSKCGFCAELAERAVEPLGGLEDAEVEPARTGRVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-29" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY6</accession>
    <name>RNP1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 1</fullName>
            <shortName evidence="1">RNase P component 1</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp1</name>
        <name type="ordered locus">MK1216</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TGY6"/>
    <dbReference type="SMR" id="Q8TGY6"/>
    <dbReference type="STRING" id="190192.MK1216"/>
    <dbReference type="EnsemblBacteria" id="AAM02429">
        <property type="protein sequence ID" value="AAM02429"/>
        <property type="gene ID" value="MK1216"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1216"/>
    <dbReference type="HOGENOM" id="CLU_107020_2_1_2"/>
    <dbReference type="OMA" id="WHELIGL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="SSF101744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000128430" description="Ribonuclease P protein component 1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <sequence length="107" mass="12135" checksum="8C0A1618430EBB42" modified="2002-06-01" version="1">MPITPRNIVRHELIGLECRVVKSLGPPYEGLEGRIVDETKNTLVLKTESGEKVIVKDQVLLELKLPSGERVRVDGALLVGRPEERLSKRIKYAEVVRGRFDPEDYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TH62</accession>
    <name>Q8TH62_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing KH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0513"/>
    <dbReference type="EnsemblBacteria" id="AAM01728">
        <property type="protein sequence ID" value="AAM01728"/>
        <property type="gene ID" value="MK0513"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0513"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.545"/>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="OMA" id="DPLAVWK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039912">
        <property type="entry name" value="PNO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="PTHR12826:SF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="94"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="202" mass="23146" checksum="1DA1FB76A80934FA" modified="2002-06-01" version="1">MAELADEFYESAERVKIPKDRIGVLIGKDGETKRYIEEKTGVELRIDSKTGEVEIRPTERVKDPLDLIKAKECVLAIGRGFSPERAFRLLREEDASLEVIDLYELVGRNPKALERQRARIIGREGRTRQLIEELSGADVSIRGKTVALIGTPRQLQIARKAIEMLASGAPHGRVYRFLEDQRRKMKREKLRLWKDSEPPDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW8</accession>
    <name>Q8TUW8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N6-adenine-specific DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1635</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02848">
        <property type="protein sequence ID" value="AAM02848"/>
        <property type="gene ID" value="MK1635"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1635"/>
    <dbReference type="HOGENOM" id="CLU_978640_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="284" mass="31810" checksum="3A2FC6D94D6950D4" modified="2002-06-01" version="1">MRPEELALKAVREALRRTVFDPDAWTHVTVVSDDALHVHILTNQRKNVEGKGGRNRQYLEGYAEGYLAAHGHDVEVRVSVVGAGLRDETEEFRYLPLKRALKEGVIEEGSPIHRVCERLGIGYVVLGAREIVGPNPALVEVVEGLEGERAVDVFTGTGTGALAAVEAGFEQVYAIDVRVHPEVRERLESEGVEVIEADFRDVDLREFEPIDLLTADPPYASTLEFLEKLSEERPRVDTAVVCHGFSSWTRAVREIRGFLIELFEDVEPVSKYGHELSVCRRLRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY4</accession>
    <name>RL31_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L31e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl31e</name>
        <name type="ordered locus">MK1617</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY4"/>
    <dbReference type="SMR" id="Q8TUY4"/>
    <dbReference type="STRING" id="190192.MK1617"/>
    <dbReference type="EnsemblBacteria" id="AAM02830">
        <property type="protein sequence ID" value="AAM02830"/>
        <property type="gene ID" value="MK1617"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1617"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1780"/>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="OMA" id="MEERIYT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_L31e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_L31e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_L31e_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="PTHR10956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="SSF54575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153795" description="50S ribosomal protein L31e">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00410"/>
        </source>
    </evidence>
    <sequence length="86" mass="9950" checksum="6E0F0852A8EA68A0" modified="2002-06-01" version="1">MAEVVDERVYTVPLRDAKKAPLKKRAPRAVKALRQFIERHMKAEEVRIGNDVNEKIWERGIKKPPSKIRVRAVKYADGTVEVRLAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY9</accession>
    <name>SRP54_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="1">SRP54</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp54</name>
        <name type="ordered locus">MK1612</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY9"/>
    <dbReference type="SMR" id="Q8TUY9"/>
    <dbReference type="STRING" id="190192.MK1612"/>
    <dbReference type="EnsemblBacteria" id="AAM02825">
        <property type="protein sequence ID" value="AAM02825"/>
        <property type="gene ID" value="MK1612"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1612"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1775"/>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000101178" description="Signal recognition particle 54 kDa protein">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="246"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <sequence length="447" mass="49732" checksum="EDE3844E1756D337" modified="2002-06-01" version="1">MIGFADRLAEITKKIKGASIIDEDFVKEVVRDVQRALLEADVDVKLVLELSKRIEKRALEEEPPAGVPKRDYLLRIVYEELVELLGGEKTEGLDIDLSRDVNVIMLVGLYGMGKTTTAAKLARYLQRKGYRVGLVGADPYRPAAGEQLRQLAEEVDVPVHVEDVDDAVEMAVKGVEALKDECDVVIVDTAGRDRLSEDLIDELREMAERIEPHEVLLVLDATVGQKAGDHAEAFHEAVQLTGVVITKLDTAAKGGGALSAVARTGAPIKFVGTGERVDDLEEFNPRSFVARLLGIGDIDELLRRTEEMLEEEEKAEDVLEGEFTLKDLYEQLEALSKMGPVDKLLQYVPGMGGGRNVRKISQITEERLKKYKVIMDSMTEKELENPEILNKSRIRRIAIGSGTSERDVIELLNHYRMMKDVIEDIQSGRIPRIGGELGRVIRNVLRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ3</accession>
    <name>RS17E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17e</name>
        <name type="ordered locus">MK1608</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUZ3"/>
    <dbReference type="SMR" id="Q8TUZ3"/>
    <dbReference type="STRING" id="190192.MK1608"/>
    <dbReference type="EnsemblBacteria" id="AAM02821">
        <property type="protein sequence ID" value="AAM02821"/>
        <property type="gene ID" value="MK1608"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1608"/>
    <dbReference type="HOGENOM" id="CLU_176720_0_1_2"/>
    <dbReference type="OMA" id="TRDFEHN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_S17e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_S17e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="RPS17e-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="PTHR10732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="SSF116820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000141554" description="30S ribosomal protein S17e">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <sequence length="70" mass="8404" checksum="6F91E9C10D803469" modified="2002-06-01" version="1">MGKVRPTFVKRPAREIVEKYEEYLTTDFEHNKKVVEIVARPKTKKLRNMIAGYVTHLMRLKERQREEGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ6</accession>
    <name>Q8TUZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-formyltetrahydrofolate cyclo-ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1605</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1605"/>
    <dbReference type="EnsemblBacteria" id="AAM02818">
        <property type="protein sequence ID" value="AAM02818"/>
        <property type="gene ID" value="MK1605"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1605"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1768"/>
    <dbReference type="HOGENOM" id="CLU_031500_2_0_2"/>
    <dbReference type="OMA" id="KTVYMAV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13017">
        <property type="entry name" value="PTHR13017"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="237" mass="26359" checksum="DA43B959BDF4A3FB" modified="2002-06-01" version="1">MTSTEKARIRRMVWEELEESGEAAPPFPVEGRIPNFKGALVAARRLTSTPEYEEAEVVKVNPDSPQRPVRERALRDGKILIMPTPRLKRGFLVVKNPKDPRRASTIRGAFQEGELTMPDELPAVDLVVAGSVAVAPDGARVGKGGGYFDLEWGILAQLDLVDEDTPIHTTVHDIQVLPPGEIPMEEHDVPVDVIHTPTGTTECVRRYEKPGGLLEDRIDEKIREIRWLREYVSAGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV16</accession>
    <name>Q8TV16_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-fuculose phosphate aldolase</fullName>
            <ecNumber evidence="4">4.1.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-fuculose-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1585</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuculose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:12933"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57846"/>
            <dbReference type="EC" id="4.1.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.17" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1585"/>
    <dbReference type="EnsemblBacteria" id="AAM02798">
        <property type="protein sequence ID" value="AAM02798"/>
        <property type="gene ID" value="MK1585"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1585"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1746"/>
    <dbReference type="HOGENOM" id="CLU_006033_3_2_2"/>
    <dbReference type="OMA" id="ICRYGRS"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="7">
        <location>
            <begin position="13"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="190" mass="20535" checksum="221A94AE49C9FC2C" modified="2002-06-01" version="1">MKVPSSSLAGLRRTVAELCKEVHRAGLTIGGSGNVSVRSGRYVAVSPSGFRLSDVRPRHVPIVDVEGREVLGTTKPTSELLMHLSLYREVGDGVVIHTHSPYLTALVHSGNRPPETEDLRRSVGEIVWVEYQPPGSERLAEAVAKVARKPMVAALERHGGLVVADRPEAALRLAEALEEAARLAVLRPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV18</accession>
    <name>Q8TV18_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase II small subunit</fullName>
            <shortName evidence="12">Pol II</shortName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="12">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">hys2</name>
        <name evidence="12" type="synonym">polB</name>
        <name evidence="16" type="ordered locus">MK1583</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EC" id="3.1.11.1" evidence="12"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1583"/>
    <dbReference type="EnsemblBacteria" id="AAM02796">
        <property type="protein sequence ID" value="AAM02796"/>
        <property type="gene ID" value="MK1583"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1583"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1744"/>
    <dbReference type="HOGENOM" id="CLU_027850_1_0_2"/>
    <dbReference type="OMA" id="HVQAALI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="PTHR10416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="OB" evidence="14">
        <location>
            <begin position="166"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol_E_B" evidence="15">
        <location>
            <begin position="263"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAM02796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="529" mass="58980" checksum="10DC7B4E0D5CBD48" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV24</accession>
    <name>Q8TV24_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1577"/>
    <dbReference type="EnsemblBacteria" id="AAM02790">
        <property type="protein sequence ID" value="AAM02790"/>
        <property type="gene ID" value="MK1577"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1577"/>
    <dbReference type="HOGENOM" id="CLU_117042_0_0_2"/>
    <dbReference type="OMA" id="ADFPYEG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="9"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="195" mass="22426" checksum="015B965B50D04D99" modified="2002-06-01" version="1">MTYGISKHLEISGEYRDKLLRGEKRATIRVGRVPGARPGKVVYIHCGGYVYGKVRITNVRTKRVRDLTDEDANLDGFENREELLKALRDHYPNLRDDDIVTIIEFEWVERFDEPILSEHLPYEGHDPIEIAKRALEEDIPLSPRDRELLELLVECGSIRKAAKALGGLGKRDVIRRAVRKAFRLLKSRGALKGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV58</accession>
    <name>Y1542_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase MK1542</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1542</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV58"/>
    <dbReference type="SMR" id="Q8TV58"/>
    <dbReference type="STRING" id="190192.MK1542"/>
    <dbReference type="EnsemblBacteria" id="AAM02755">
        <property type="protein sequence ID" value="AAM02755"/>
        <property type="gene ID" value="MK1542"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1542"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1703"/>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156394" description="UPF0173 metal-dependent hydrolase MK1542">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="230" mass="24762" checksum="831B219401AE17CF" modified="2002-06-01" version="1">MRITWLGHAAFEVEIDGVNVLIDPFLSGNPKAAKSPDEVDPDLVLVTHGHGDHLGDAVEICKRTGATLVGIYEIAVYAQGQGVENVEEMNIGGTIEVEGLEITQVPAWHSSEIVEDGEIVAGGTPVGYVVSGEEGSVYHAGDTGLSMDMKLIGELYEPEVALLPIGSRFTMGPKEAAKAVELIEPEVAIPMHYGTFPPIEQDPEEFKREVEELGLDVEVVILEPGESYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV94</accession>
    <name>Q8TV94_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaea-specific kinase related to aspartokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1498"/>
    <dbReference type="EnsemblBacteria" id="AAM02711">
        <property type="protein sequence ID" value="AAM02711"/>
        <property type="gene ID" value="MK1498"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1498"/>
    <dbReference type="HOGENOM" id="CLU_089197_0_0_2"/>
    <dbReference type="OMA" id="WRFANIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04240">
        <property type="entry name" value="AAK_UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011375">
        <property type="entry name" value="MfnE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004857">
        <property type="entry name" value="Kin_aa_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="1">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="22513" checksum="2E3E1D945B195DEE" modified="2002-06-01" version="1">MKVVLKAGTGAVKEVDAVKNAITAFEGELLVVPGGWRFANIVREVYEDGDLSDDAAHWMAIAAMDQTGYLLSDLLDLPTTEEPEFGGKLVLLPYRYLRMKDPLPHSWEITSDAISVYVAAEANANLVVFAKDVPGILEDPDDPSSLIREIDARELEGNWTALDPVAPRLAEEYDLELRVVYAGNPDNLLRAMRGEEFVGTRVVPG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD7</accession>
    <name>RS27A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S27ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27ae</name>
        <name type="ordered locus">MK1455</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVD7"/>
    <dbReference type="SMR" id="Q8TVD7"/>
    <dbReference type="STRING" id="190192.MK1455"/>
    <dbReference type="EnsemblBacteria" id="AAM02668">
        <property type="protein sequence ID" value="AAM02668"/>
        <property type="gene ID" value="MK1455"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1455"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1611"/>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="OMA" id="GVFMAEH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_S27ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_S27a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_S27ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000137695" description="30S ribosomal protein S27ae">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <sequence length="60" mass="6876" checksum="132DC46B4F998E2F" modified="2002-06-01" version="1">MGVPRRAKLYEVKDGKVERKNPFCPRCGPGVFMADHGNRYACGRCGYTEFKDQPEPKKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD8</accession>
    <name>RS24_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps24e</name>
        <name type="ordered locus">MK1454</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVD8"/>
    <dbReference type="SMR" id="Q8TVD8"/>
    <dbReference type="STRING" id="190192.MK1454"/>
    <dbReference type="EnsemblBacteria" id="AAM02667">
        <property type="protein sequence ID" value="AAM02667"/>
        <property type="gene ID" value="MK1454"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1454"/>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="OMA" id="QYIRSYF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_S24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_S24e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="PTHR10496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137644" description="30S ribosomal protein S24e">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="116" mass="13736" checksum="BDD6E1ED22D9769F" modified="2002-06-01" version="1">MEVEILDQRDNPLLYRKEVKFVVRHEDSGTPQKSEVLRKLAAILDVDKEVVLIDRMESEFGKRETKGYAKIYKSMEHLEDIEPEHMVERHKKVLEELESESEESEESESEESEEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD9</accession>
    <name>DPCKG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="1">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1453</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + GTP = CoA + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVD9"/>
    <dbReference type="STRING" id="190192.MK1453"/>
    <dbReference type="EnsemblBacteria" id="AAM02666">
        <property type="protein sequence ID" value="AAM02666"/>
        <property type="gene ID" value="MK1453"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1453"/>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="OMA" id="AIYDHKT"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="PTHR40732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137609" description="GTP-dependent dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <sequence length="191" mass="21599" checksum="D8B1DA81587F2F7E" modified="2002-06-01" version="1">MTVVLRLPRELRPELRRPWGTLYPRPSIKTYRRLHEESEVLITVGDMTTRSFLRCSIRPDVAVVDRKMLRTVPVDPGNKFPVTLDVNNPPGTITREAWDTVRRGIDYALDGDATLIDVTGEEDLLAIPAILIAPENSIVCYGLPGEGMVAARVTQHLKDSVLRLLTRFRGYDEWKSRSWISGITPCCTAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE0</accession>
    <name>Q8TVE0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpoE2</name>
        <name evidence="2" type="synonym">spt4</name>
        <name evidence="4" type="ordered locus">MK1452</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1452"/>
    <dbReference type="EnsemblBacteria" id="AAM02665">
        <property type="protein sequence ID" value="AAM02665"/>
        <property type="gene ID" value="MK1452"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1452"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1608"/>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="OMA" id="VDRCPYC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="PTHR40704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4" evidence="3">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="64" mass="7188" checksum="6C638870AA1F25D4" modified="2002-06-01" version="1">MSKLKACVRCGYLVEEDTEICPACHGDEFTENWRGIAVILDTESQTADRLNAKIPGKYALRVEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE7</accession>
    <name>RS28_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S28e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps28e</name>
        <name type="ordered locus">MK1445</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE7"/>
    <dbReference type="SMR" id="Q8TVE7"/>
    <dbReference type="STRING" id="190192.MK1445"/>
    <dbReference type="EnsemblBacteria" id="AAM02658">
        <property type="protein sequence ID" value="AAM02658"/>
        <property type="gene ID" value="MK1445"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1445"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1601"/>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="OMA" id="MHGEASQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_S28e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_S28e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="PTHR10769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000136849" description="30S ribosomal protein S28e">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <sequence length="74" mass="8232" checksum="5074C8540A633BA4" modified="2002-06-01" version="1">MVECDYDPTEDATPAEVVEILGRTGMAGEVTQVKVRILEGPDKGRIITRNVKGPVREGDILLLRETEREARPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF3</accession>
    <name>Q8TVF3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-13 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02652">
        <property type="protein sequence ID" value="AAM02652"/>
        <property type="gene ID" value="MK1439"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1439"/>
    <dbReference type="HOGENOM" id="CLU_380663_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="796"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="25"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="435"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="821" mass="92218" checksum="542D683B6FD8978B" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG8</accession>
    <name>Q8TVG8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1421</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02634">
        <property type="protein sequence ID" value="AAM02634"/>
        <property type="gene ID" value="MK1421"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1421"/>
    <dbReference type="HOGENOM" id="CLU_803185_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="345" mass="39772" checksum="C2141BE6DF7F3F65" modified="2002-06-01" version="1">MFKSRRLPSFLIEGKVDEELEDFTDDFGQPDLDAIVRACVDRLDPHPDLEERVLKTARKACERIGFPMGEHAEFDLYPWLAPQIFHERVVRDVYSGESREPGEARVAFVSVHHDKRGVAEYVYRTLERLEPEAVCLETSPAGLETAFHYTLSPLPHAGIEVMARVPTKMLMGPFRDLYGALVYCLREGVPAIPVDVPSSLKLKESRKNPECLRDPIVFEYTSEVERVLGPTGELFFREYEEVEPVLRRLGGEIRTNVSLVASETFVRFHLEFRERYMLSRIADVTEDFDRVAVVVGAVHTSAMERAWREGEFRYLEPSPEDFKGVEWELSPRYKRHKLVVGGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI2</accession>
    <name>Q8TVI2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK1407</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0718 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02620">
        <property type="protein sequence ID" value="AAM02620"/>
        <property type="gene ID" value="MK1407"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1407"/>
    <dbReference type="HOGENOM" id="CLU_049002_0_0_2"/>
    <dbReference type="OMA" id="IPMYICA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005524">
        <property type="entry name" value="DUF318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03773">
        <property type="entry name" value="ArsP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="333" mass="34398" checksum="40AE7E519421E2F0" modified="2002-06-01" version="1">MSGGRRLDPLGAFVGLLTEISPYLLIGLVLGAALQSMVPERYLARHLRGGLKACIIATLLGVPLPLCSCSVLPLAIAAKNRGAGVGPVIAFIVATPQTDVNSLLLAYALLGPYFTAAKVLTAVVAAVIVGYLAELTLKDRSEETEELPSCGEHGPCYRGRSFKDFPRELLELSGTVGPWLLIGLAASVLLELWVPSPAVRYLEGFLGPVLAALISLPLYVCSVAAIPIAAALVAKGASVGSMLTFTVAGPGTNVATLSVILRFLGGRIVAAYLVGITLCAVAAGYAADTIGVPVVSTGIFEASCHPSLGAVSAAILLLLMTAGIIWRLVRDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK1</accession>
    <name>Q8TVK1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted calcineurin superfamily phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1388"/>
    <dbReference type="EnsemblBacteria" id="AAM02601">
        <property type="protein sequence ID" value="AAM02601"/>
        <property type="gene ID" value="MK1388"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_075478_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
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        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="13"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="231" mass="25624" checksum="65BF9CADC26C281D" modified="2002-06-01" version="1">MELYHEVIMLKDIAALADVHVGVEIELRRRGIRAVDRTEDRVEKLRRCLEALDPSILVIVGDLKHNVPFASRIEFRGVPKLVDAALEIVDEVIVVKGNHDGLVEELLRNQRGVRVVGTRGILIDGFYFLHGHAEPDPELLSRSDLLVFGHEHPISDAVPGVSVKVLVELELDFEELTRGEVSGRGPGFVLPAFDDLVGGTEVTSDDRLLLAHRRGAVIDESYLPIPEAEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK8</accession>
    <name>Q8TVK8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1381"/>
    <dbReference type="CAZy" id="GT81">
        <property type="family name" value="Glycosyltransferase Family 81"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02594">
        <property type="protein sequence ID" value="AAM02594"/>
        <property type="gene ID" value="MK1381"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1381"/>
    <dbReference type="HOGENOM" id="CLU_478695_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="28"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="555" mass="61531" checksum="94FF4BCE33CBA6EA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM5</accession>
    <name>Q8TVM5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein specific for M.kandleri, MK-13 family, a frameshift</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02576">
        <property type="protein sequence ID" value="AAM02576"/>
        <property type="gene ID" value="MK1363"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1363"/>
    <dbReference type="HOGENOM" id="CLU_2420057_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="2"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="706" mass="78604" checksum="9AD2CCA882940B5B" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN3</accession>
    <name>Q8TVN3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-10 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02568">
        <property type="protein sequence ID" value="AAM02568"/>
        <property type="gene ID" value="MK1355"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1355"/>
    <dbReference type="HOGENOM" id="CLU_910936_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="278" mass="30802" checksum="0AD16E7F4A821AC5" modified="2002-06-01" version="1">MLEPLTLTLLALPGDPGDVLGPVLEHPEVFPAMGRKGHDGERWTGRRKRVQRVDAKLSRVGEILSGRSPGTRKGSELRKRYPERKRERLLSLYRRVRLYYRTVFDELVRLLVALLVTFFMVCLAVKAAGEDSVANLFRLAGISIIVDIVALIAFCEIVLTAVEDFADAVLAGESPEAALDAFYGGAVLTLGYSLIAYAAVALWIVRRTSRELDVRTLVQASILILPLVAGFRALVWGMWVTGRYADVVPQLLGFTSVLIVIYLLVRLNLKLSKDRASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP1</accession>
    <name>Q8TVP1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02560">
        <property type="protein sequence ID" value="AAM02560"/>
        <property type="gene ID" value="MK1347"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1347"/>
    <dbReference type="HOGENOM" id="CLU_2504957_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="85" mass="9090" checksum="49DD6956EFF74008" modified="2002-06-01" version="1">MAKMVNSAWVRLKSHERKLEKARTVERVGRFRLNLATLGAVVGLALAGLSVLQPTLLGTLGTFLSILAQVVTLAVQLASLIPLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP3</accession>
    <name>Q8TVP3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1345</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02558">
        <property type="protein sequence ID" value="AAM02558"/>
        <property type="gene ID" value="MK1345"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1345"/>
    <dbReference type="HOGENOM" id="CLU_009273_4_2_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="285" mass="31817" checksum="29398D0733386CB6" modified="2002-06-01" version="1">MSFHVTVDVTTECPHDCPHCNLRGTRRAGHLDPEVLERILAEVADRYDLDFVILTGGEPCLHPRFGELVELCRRYDAGVGVNVATEVHPALADVDHVYVALEPHRGYGLEGALRTWEEVARLEPDEVYANTVACRDTYGELRRVNEVAVELGAAAHFVIAYVPPGPDDPLDDYPHDELPGLVRGLRRACLAGMPYQEEVPFSSCRHGEYNLHVRADGELTPCQHWKTYTLADLDEFEGMRGLEPEGPCRDCPRFEECQGGCNAYAWNVAGRLMPDPRCPRVRGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP9</accession>
    <name>Q8TVP9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, contains two domains of the MK-3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02552">
        <property type="protein sequence ID" value="AAM02552"/>
        <property type="gene ID" value="MK1339"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1339"/>
    <dbReference type="HOGENOM" id="CLU_331946_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="760"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="790"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="862" mass="95824" checksum="457BF22E29C1B5D6" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ1</accession>
    <name>Q8TVQ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02550">
        <property type="protein sequence ID" value="AAM02550"/>
        <property type="gene ID" value="MK1337"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1337"/>
    <dbReference type="HOGENOM" id="CLU_2893261_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="62" mass="7352" checksum="3D85A73967639022" modified="2002-06-01" version="1">MLRLIKRILWGILSYLHEIIYLEELLLAIVDRTIVVLLLPIRLPYRLLETVRRALRRGLTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ5</accession>
    <name>Q8TVQ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02546">
        <property type="protein sequence ID" value="AAM02546"/>
        <property type="gene ID" value="MK1333"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1333"/>
    <dbReference type="HOGENOM" id="CLU_833167_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="333" mass="36980" checksum="054D249BD2F1A149" modified="2002-06-01" version="1">MNTGKAWRESLPPEEARKLLDLAREDLAKIPQLKSGELEDESGLRLVVYDVTEDKVLLLLARPSLEFIRGHNPRLAELLHVLESTADSLLEWDIFQLSARAREVKDLLEGAGLASTGAIRYLLHPELALGTFLLTSGFGFIAFLPWIELLKRFPRLGEAEFRWEPSALLEYARRLAYVGVLYGAELAYWTCCEIAVKALLRVLGLWDYTVNGRIFDLADSDVWAPLLEEPTKRLMSELSGLPRPLVGAWYGLMEAVTKREIGPGGVNVRNAVLRVVGHAVCATLPLPLGALLHSLWADECDFGDRFLEYWPGVLAPVLAVLGALAENALTGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR5</accession>
    <name>Q8TVR5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase of the beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1323"/>
    <dbReference type="EnsemblBacteria" id="AAM02536">
        <property type="protein sequence ID" value="AAM02536"/>
        <property type="gene ID" value="MK1323"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1323"/>
    <dbReference type="HOGENOM" id="CLU_036012_1_0_2"/>
    <dbReference type="OMA" id="IVISHTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="216" mass="24280" checksum="BA80F4AB65A0E587" modified="2002-06-01" version="1">MRVVLVYDDRRGRRGFRPSWGFSCLVELEGRRLLFDAGGRDRILLHNLQEAGVEPRDVELVIVSHDHDDHVGGLPGLLDGNPGITVFASPSSELDVRRVNRVRNREEVVSGVLAVERPNNVGLLLESEEVLLAAREPGELVELVRWVCERWEVETVIAGFCRYPGAARIRQVAEVLGELGVGRVVGCHYFRPSDARHFERWGIECERPGVGEELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT3</accession>
    <name>Q8TVT3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-16 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02518">
        <property type="protein sequence ID" value="AAM02518"/>
        <property type="gene ID" value="MK1305"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1305"/>
    <dbReference type="HOGENOM" id="CLU_557379_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="394"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="509" mass="55589" checksum="356A03B6ACDC3C9D" modified="2002-06-01" version="1">MGFLSDAIDKVIRGVRRGRADLKSVVRSALLFTPVWSVITLLALLESMSFNDLPRLSFGLIVGLLSSSPFQVLGSRMVADARYLGVLSPVIGVLALLAVVYPPVPYLLSRLLSESFGLKLSHDVVLAAELTGIVYTLTCMVAASWKEYRWKAVLQIVASWTAFSVAFAVLAAVTSDVLRSFVLSSSLLAITLTASLVANARKHLENPPEITKEYLVGCLVVAAAAALDYFVIALSSIAYYAMMFFDKIVIWCGRGFPYPPLDVPASVGMVPLLAGTFAAGIFWIDVGDDLDRIYRVESRELEEIGRRMFLQFIKGAGIALALSGVLALVTASFVRPRFHYWMSGDVAYLLGPVLKASSALIHAVSAVPVPPFGNLATLVKWIERQLAGANPDLLVLYLLGSVPGALVVYTYPQLITFRREIEATVALSLVPIAELLAWHRWGVKWLALGYLMGTCASATVCALTAVRMWLNLHTEAYRVLSYNNFVSYVIEVQRHGGEEVRPRDPTIRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT5</accession>
    <name>Q8TVT5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02516">
        <property type="protein sequence ID" value="AAM02516"/>
        <property type="gene ID" value="MK1303"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1303"/>
    <dbReference type="HOGENOM" id="CLU_791346_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="350" mass="39027" checksum="687FC75CE4507E38" modified="2002-06-01" version="1">MRVRWVLLPALLALVLPLPVHGDAWVGPEALLDVGVQSDLIIEDVHLKESDHGVEVVDHVDAEYRAYIRVKEPGKPVTLHVLLPQGTEVLEARFYPELYPIEGIDLDSPDVNVKWERIEPVSRSEVTWKVLGWTWRWTQLDFRVTPKYDERRHGCSALVLKLRIPTAHREPEEIDRLPIAGPKHYPELGSYYYFGCIEWPAPLQPSGRGGGFNCWATSDAWSPHVLFLWPGGFADIQEGFTNVSLTDHLVLTLGGSGGGSGAAVDVVGVREEGERVVQSEWPRVTGRFGFPYVPVVAVIGLEKKEPSSPKTGRKPEREKPAKHELPVPPIVPPPMRGRRRLTGKASNSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT8</accession>
    <name>Q8TVT8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02513">
        <property type="protein sequence ID" value="AAM02513"/>
        <property type="gene ID" value="MK1300"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1300"/>
    <dbReference type="HOGENOM" id="CLU_1682707_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="156" mass="16166" checksum="ED4EC8B3FDFFA852" modified="2002-06-01" version="1">MPAPVRSVSFGRPSGIEGTQTGGEEGTGEKPEEKEGTEEKPGEEKPANGVGGGTETDRTRETSTKPEGPGKPTGGTEGTGGATESSKNGTEGEPKPSEPEKPDGGTGKGRKNGGESEGAEGVKREPQPREPKGRRLPVWWVLPVPTPAALRRWISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU2</accession>
    <name>Q8TVU2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted component of a thermophile-specific DNA repair system, contains two domains of the RAMP family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1296</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1296"/>
    <dbReference type="EnsemblBacteria" id="AAM02509">
        <property type="protein sequence ID" value="AAM02509"/>
        <property type="gene ID" value="MK1296"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1296"/>
    <dbReference type="HOGENOM" id="CLU_396715_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010172">
        <property type="entry name" value="CRISPR-assoc_prot_TM1791"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39965">
        <property type="entry name" value="PTHR39965"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01898">
        <property type="entry name" value="cas_TM1791_cmr6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="564"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="388"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="694" mass="77474" checksum="8EF4B4E9D8EB353D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU3</accession>
    <name>Q8TVU3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1295</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02508">
        <property type="protein sequence ID" value="AAM02508"/>
        <property type="gene ID" value="MK1295"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1295"/>
    <dbReference type="HOGENOM" id="CLU_870479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="368" mass="39638" checksum="64288E9A64280D0F" modified="2002-06-01" version="1">MTVVPLVLGLILTATPSAGEPLIRGKASDDIGYLARYFESVVEPKWKSADARIVVDARGFRGRTVDVVYVPGLDAFVVPAGGRPRGSVTSVSDVLPLSYVECEPGKVRIRLDRVGRTVVVEGPAVVVRGLYPPIPLDVSALVDGYRCTVVGGVQLPIDGGCPLAPRPYVPPAAVLEGGGSSDRVVLADGPWPLLDVEYLVGGDVVNMRVQLLEDPLTSALELAIATVSHRKADVAPLQGVEVTFDLGSGKIRTRHVAVPWRPTGDTSEDGVGTLKGGVVMRTADGLEPLLVWFEVARDDPMMEFISDVVEAYRRGDFGEVGRLMKAHPNVVFLFPEGREGFWVYAPQVVPLRRAHRRRIAGTRRRGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV9</accession>
    <name>Q8TVV9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1279</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02492">
        <property type="protein sequence ID" value="AAM02492"/>
        <property type="gene ID" value="MK1279"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1279"/>
    <dbReference type="HOGENOM" id="CLU_1324029_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="22425" checksum="F22AE643E37DD09B" modified="2002-06-01" version="1">MVLPLVTAILLTAAPAAGLQFEAPTAGYPVPEPPHLEDGDGHVEPPFRYAGDHRVRVYPCSVPSTAWVFLEDRRCVIAVKPVGGGHPVLLLDVSDLVVSVGADGVRLRGGYATAVRMEPVDFPSLWIPYEVVTGSGVELLFPWLLGPIVRECSRTEFERVAELIAGERGTPPEDVETGVWDELRPPSPLPVPAVLFVPPARKRRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY0</accession>
    <name>Q8TVY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted hydrolase of the metallo-beta-lactamase superfamily fused to a uncharacterized domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02471">
        <property type="protein sequence ID" value="AAM02471"/>
        <property type="gene ID" value="MK1258"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1258"/>
    <dbReference type="HOGENOM" id="CLU_830582_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="32"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="375" mass="41680" checksum="EACAD910F1E65144" modified="2002-06-01" version="1">MGIQRLAHNSPYSHHRGVKCSGDGGVLLRPADSWAGVLVDTGSTIVAVDAGPNPWHAEDTDYLLLTHAHLDHVASLDRYSRNGAKVLCPGDVRDDLGLVDSAVGVKKVKSMLYDVIVHPIPVEHTCSAYAYVLDLEECNVLVTGDWHVGPRTIDGEIKPLWKAVREVVGGEGVDVIVSEATRALVDAPELGGNERVFEYALSLHDPRDVLAFLQPTDLEIIDTAFRVASDLGLDVVVDSETDRKLKFLERRGDVEWEYDVADEPLVRSLYLTVSWEKARELAEAGLVEAVVGNWATVHHLRRRVGVKYAYVIPRSGHASRPEITRLITELEPSCLVFRHRSGDATRLERYYSRFVDEVRVWVGRGCRDESLELPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ8</accession>
    <name>Q8TVZ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MinD superfamily P-loop ATPase containing an inserted ferredoxin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02453">
        <property type="protein sequence ID" value="AAM02453"/>
        <property type="gene ID" value="MK1240"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1240"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1344"/>
    <dbReference type="HOGENOM" id="CLU_067767_0_0_2"/>
    <dbReference type="OMA" id="DVEEPNC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="262" mass="28680" checksum="DCBD369929D38912" modified="2002-06-01" version="1">MWLAVTGGKGGVGKTTVAVHVHRILGWDALDLDVTTPNLHLYLDCEEVERSDVYIPCPKLEDEDACDLCGTCARVCAPGALVVGRSWDLDPDLCHGCGLCIESCPNGALAYDRVRIGEVRRYRVSETGATLTSGSLDVGDRRSRHLVHALLEGADDERDLILDTPAGAGKDVYDVLKAADAAIAVTQPTPAAYHDLRRLMRVADRAGIEVVILINRSDLSDSWRRRIEEEVRDPVVEAPTVDDPPRSEVFREAVERCLEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ9</accession>
    <name>Q8TVZ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MinD superfamily P-loop ATPase containing an inserted ferredoxin domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02452">
        <property type="protein sequence ID" value="AAM02452"/>
        <property type="gene ID" value="MK1239"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1239"/>
    <dbReference type="HOGENOM" id="CLU_067767_0_0_2"/>
    <dbReference type="OMA" id="GIGCVVI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="259" mass="28009" checksum="DC633EF3E86622C8" modified="2002-06-01" version="1">MDPELKVAVLGGKGGVGKTMLSIALAAELGVPLVDADVTEPDVPAYLELEVEEVLEEIRARYAVKTDDCVECGRCSEVCPWDAVEDPTACDGCGICAIACPEDAIEFEPEVLGEIVRSRNPDLNLEVIHPRSEPWVPELAHVIHRMVPERPCVIDCPAGLGCPVIAALSRADAAVLVTAPSPAARHDFERARTLVERFGLPTVTVLNRADGSSDDLDVVIPESEDVLDAALRRDARALYRALRSHAREIVEELRSEASF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-06-07" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW13</accession>
    <name>RL32_METKA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L32e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl32</name>
        <name type="ordered locus">MK1225</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW13"/>
    <dbReference type="SMR" id="Q8TW13"/>
    <dbReference type="STRING" id="190192.MK1225"/>
    <dbReference type="EnsemblBacteria" id="AAM02438">
        <property type="protein sequence ID" value="AAM02438"/>
        <property type="gene ID" value="MK1225"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1225"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1328"/>
    <dbReference type="HOGENOM" id="CLU_071479_3_1_2"/>
    <dbReference type="OMA" id="HPSGYEE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_L32e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_L32e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_L32e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_L32e_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="PTHR23413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="SSF52042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131152" description="50S ribosomal protein L32e">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="131" mass="15699" checksum="E46A144888168B7E" modified="2002-06-01" version="1">MVRNPNLSKEEERLLKLREELKRKKPKFRRQEWHRYKKLGEKWRRPKGRHSKMRRKLKSKPKMPNPGYGSPKKVRGLHPSGYEEVLVYNPKDLEKIDPKRQAARIASRVGRRKRQEILEKAEELGIVVLNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW16</accession>
    <name>RS14Z_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14</name>
        <name evidence="1" type="synonym">rpsN</name>
        <name type="ordered locus">MK1222</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW16"/>
    <dbReference type="SMR" id="Q8TW16"/>
    <dbReference type="STRING" id="190192.MK1222"/>
    <dbReference type="EnsemblBacteria" id="AAM02435">
        <property type="protein sequence ID" value="AAM02435"/>
        <property type="gene ID" value="MK1222"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1222"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1325"/>
    <dbReference type="HOGENOM" id="CLU_177289_2_2_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_S14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="40S_S29/30S_S14z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_S14_type-Z_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="PTHR12010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000269161" description="30S ribosomal protein S14 type Z">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <sequence length="48" mass="5687" checksum="409692A70546FAA5" modified="2002-06-01" version="1">MARREFGKGARRCRRCGDTHGVIQKYGIMLCRQCFREVAEKMGFKKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
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        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
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    </dbReference>
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        <property type="entry name" value="GTP-bd"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MMR_HSR1_Xtn"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GTP1OBG"/>
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    </dbReference>
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        </location>
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            <end position="66"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="388" mass="43172" checksum="C34F2F566811C041" modified="2002-06-01" version="1">MPANLPPEYHELEEKYRKARSPEEKLRITEEMLAVVPKHKGTENLRALLKRRLAKLREEVEKRRQQKSGGGPDYNVKKEGAAQVALVGPPNAGKSALLRELTNADPDVASYPYTTKEPVPGMMEYKDVQIQLVEIPPIYEGFTRGDGSKFVGVIRNADALCLVVDLTEDPVEQLETVLRELESAAIKLNQDPPSITIEERVTGGIEIRGEDRLDCDPNDVKDLLRDAGIHSAVVVIEEDGITLEDIADALDKRIEYKPALLVANKGDAPGSKESYERLVERVEDLEFDLPVVPVSAEKGINLDEFKRRLYDTLGIIRVYTKKPGGKPQKPPIVLPKGATVEDVAREIHSDLAKNLKYARVWGKSVKKDGMMVGRDHVLEDGDVVELHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
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                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAM02407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="180" mass="19963" checksum="8935B0CADA923F75" modified="2002-06-01" version="1">MSITETLVAYFMDFERAMGLPGMLVITALECSVLPVPPEPFVFPFAMRMDPWVLATLVTLSSIVGSLLGYAIGYFGGRPIAVRLVGEANLMRVESKLTEHRFSAWTAVFLAGLLQFIPFKPFTIGAGLVEMDLRLFITAVVTGRFSRFLFLGYVAQSETVRNWISYYLGPKALKMLMSTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW75</accession>
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        <submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAM02374.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="221" mass="26122" checksum="956417654C3C2E51" modified="2002-06-01" version="1">MFSARQLTQPLHVLPSIILMYLPIVSIPHAELTDSSVFLTFLMIVLGVVFVVSISMFLSFPFAMILRVKDSRVWILSWLYCETELITDGSIEVSGFREFTSYPVEYEDLPAGSPRDLMVLIGYFKPELHRIEIRDCNFFFLRTRSFCLLYDACLPGDREEAFRLARWWERFSMVYEALFEDEIPDEKNIMKYLDGLYQFKAAHYYVPLPRWPYDEDRDARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW79</accession>
    <name>Q8TW79_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1157</name>
    </gene>
    <organism evidence="1 2">
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        <lineage>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02370">
        <property type="protein sequence ID" value="AAM02370"/>
        <property type="gene ID" value="MK1157"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1157"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="24555" checksum="7568CEF31FB88F0B" modified="2002-06-01" version="1">MVTSHVPIPHDPVERIRALRVLREVYRRRKKPSLEVTYRTVNGSTCGPYYVARWRRDSKFEHGRTLYLGKPENESVSFVEWLVSLDREEVLELARHLMRNLRSVLKTLLTEVSDLPYKRARRVLARGLALTFDARPSDSPRIRDVLEKLPDRLESFLIRALGSWPAHYSSHLRKVIRSRRESLDGKHEIPDVEFELERWKLRHGRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW92</accession>
    <name>Q8TW92_METKA</name>
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        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1144</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02357">
        <property type="protein sequence ID" value="AAM02357"/>
        <property type="gene ID" value="MK1144"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1144"/>
    <dbReference type="HOGENOM" id="CLU_684455_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="402" mass="44679" checksum="3AE9925DAD7D058F" modified="2002-06-01" version="1">MALEPGQIIAFITLFSILFSIIVITPPLLVVLARGLARREARCRRETLDDHVILRSYPILIPPGLSMVSSYIVFLISILVECGVCIVTKEKHLHFLRLTAILIIVTITASLSLWLAAMTPYITIAKVLPSNHKIRIKLFTITYQPILIASFSENIHTNNIQLELKTRGSELIKYSISKRTERLLRTLFSTLDGVERVNFYLGVLKPGSSIIEVKHNPENGNIVSVITPGITLTTSQSNTNLDKVYNFDALATVTLPLLLGRNSYTSTLRDIEEKTNVSLDRISLLEWLIEFAVVGSKGVSLVESSHRIVKSNGLMLLSSLHPAFTPIIVYVLSLVIVYYIICTILKASSDPLHAILFYVIVPQLASVAVSTGISLVISPITSKRKVLDLLTEVMYLYKARRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA7</accession>
    <name>Q8TWA7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted protease or amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02342">
        <property type="protein sequence ID" value="AAM02342"/>
        <property type="gene ID" value="MK1129"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1129"/>
    <dbReference type="HOGENOM" id="CLU_1048118_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015834">
        <property type="entry name" value="UCP016642"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016642">
        <property type="entry name" value="UCP016642"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="DJ-1_PfpI" evidence="2">
        <location>
            <begin position="64"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="265" mass="27776" checksum="27FF4CC49B90CDB8" modified="2002-06-01" version="1">MRLPAIAAGLLILFAQPASCLKVAVFAADDVEPTCLKAIEKVLRDAGVPFDEVKGKDIVDGTIVERYDVLILPGGAYSERVVHHPRFIEGLKEFARAGGKIVGICAGAITLVKGGLVRAKVEGAGLGVGKVTLELERDPLTEGLPDRLEVTYINGPVMRSQGAKVVARYAGGIVSRGDAILVDRYGKGEVIAIGPHPCHDERGNVNTQGAKLLLNALGVKKRVKSGKVEGEGWFPTPVPVAAVILGLVAALGVRGLISRRGTGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC0</accession>
    <name>Q8TWC0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri with repeats, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02328">
        <property type="protein sequence ID" value="AAM02328"/>
        <property type="gene ID" value="MK1115"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1115"/>
    <dbReference type="HOGENOM" id="CLU_459027_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="594" mass="64440" checksum="31F963D3C02EE9DA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE7</accession>
    <name>Q8TWE7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1088"/>
    <dbReference type="EnsemblBacteria" id="AAM02301">
        <property type="protein sequence ID" value="AAM02301"/>
        <property type="gene ID" value="MK1088"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1088"/>
    <dbReference type="HOGENOM" id="CLU_907969_0_0_2"/>
    <dbReference type="OMA" id="HQFPYYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="15"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="306" mass="32684" checksum="4C859799B957E741" modified="2002-06-01" version="1">MDVTKLVIHYLLVIAKVAPLVFLGFFLASVMIILRVHERLGKLTGRRLARLGLTPEAASAMAASLVSPSAGYPILAEFRREGRLDDRDVVLLVVATTFPTTVGEMFLKGPFFAALAILGPKLGTEYMGALFVTALLQTLPALALYGARSGNGSDIHLPSTSHDDVPPLREAVIEGLRRAARRMKYVLPRMVGIGLPMVILAEVLRSWVHGGLGPVVAITLANVSHYTVGYATAAELVHRGVLSESEAVAALLIAGCANVLMIFLKASLATYVSIFGSRLGLRAWAANLGSSVGARLLMAYAILRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ2</accession>
    <name>Q8TWJ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02255">
        <property type="protein sequence ID" value="AAM02255"/>
        <property type="gene ID" value="MK1042"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1042"/>
    <dbReference type="HOGENOM" id="CLU_2613657_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="78" mass="8564" checksum="C7D49077F111656E" modified="2002-06-01" version="1">MSTTPLPNAEEVGRELGIPVHVHRIPKDPTSIGNLDWDGISRDVRSADVLVIQRMGTVPTAFTRELSRRTLGTALDDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK0</accession>
    <name>RNZ_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Z</fullName>
            <shortName evidence="1">RNase Z</shortName>
            <ecNumber evidence="1">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnz</name>
        <name type="ordered locus">MK1033</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWK0"/>
    <dbReference type="SMR" id="Q8TWK0"/>
    <dbReference type="STRING" id="190192.MK1033"/>
    <dbReference type="EnsemblBacteria" id="AAM02246">
        <property type="protein sequence ID" value="AAM02246"/>
        <property type="gene ID" value="MK1033"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1033"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1085"/>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="OMA" id="GTQRQMM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000155925" description="Ribonuclease Z">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <sequence length="279" mass="31073" checksum="5AA960DB93F0D17B" modified="2002-06-01" version="1">MDRELVMRFLGTGGAVPSKDRSHPGLLVEFSGTKLLIDCGEGTQRRAMEQGVTIHDVDAVLLTHHHVDHVAGLLPLATTVDLLHGRRLKVYGPTAGSESALDISDLEVIEYREVNPGDEVEIGDLRVLVYESEHGVPTVDYRIETPKIPGKADPKYIRRVPPSKRREVLLRGERPYSLTKPGKISVYVKGDGRPADPENVRGCQVLVHEACFEDHEEAVRYLHSTHLEAAEVAREAGVDLLVLTHLSTKVDPERMREEAREVFPVVVVARDGLMVRVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT2</accession>
    <name>Q8TWT2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted phosphatase homologous to the C-terminal domain of histone macroH2A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0950"/>
    <dbReference type="EnsemblBacteria" id="AAM02163">
        <property type="protein sequence ID" value="AAM02163"/>
        <property type="gene ID" value="MK0950"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0950"/>
    <dbReference type="HOGENOM" id="CLU_046550_7_2_2"/>
    <dbReference type="OMA" id="HYGKGLP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="182" mass="19522" checksum="817AB577A2B76E55" modified="2002-06-01" version="1">MELERVAEFELEDIKVVVLRGDITELNADAVVNPANSRGVMGGGVAAAIKAKGGEEIEREAMEKALIPVGEAVETTAGDLDAEYVIHAPTMERPAQRIGVENVREATEAALRKAEELGVESVAFPGMGTGVGGVPYEDAAETMVEVIERLAPELESVRAVYLVGYEEELAEAFRRALERRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT8</accession>
    <name>Q8TWT8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type multidrug transport system, ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0944"/>
    <dbReference type="EnsemblBacteria" id="AAM02157">
        <property type="protein sequence ID" value="AAM02157"/>
        <property type="gene ID" value="MK0944"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0944"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="REKQHDP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="26224" checksum="082435ACE376ECBD" modified="2002-06-01" version="1">MPPAIVAEDLRKEYGEVVALRDVSVEIEAGELVAILGPNGAGKSTFIKIVCGVTRPTSGRIEVLGGDPADPEIKRRVGIVPQQGGLYPEQTVRENLRFYSKLYGREPDPEPLEMLEVDRFMDRKAGQLSGGMRKRAAIAITLALEPEVLVLDEPTNELDPMARRDVIRVTKEFHRRGTTVLYVSHDVYEVEQLRPDRVLIFLEGRKELDEPFDEVMERHGSVLEAYEGVASCPGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT9</accession>
    <name>Q8TWT9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type multidrug transport system, permease subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0943</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02156">
        <property type="protein sequence ID" value="AAM02156"/>
        <property type="gene ID" value="MK0943"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0943"/>
    <dbReference type="HOGENOM" id="CLU_039483_2_3_2"/>
    <dbReference type="OMA" id="ANIYRLG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="177"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="371" mass="40955" checksum="F2C0BF9EEFD49188" modified="2002-06-01" version="1">MSWRVIAVRDLRAWLKDRRRLAPTLIMPVITILVMYGAFHDVKPRHVTVAVLSHSSDFDPVLDALKSDDRVGVVHVRSVDEGKTMVKEGRAVVFVYVPKGFPSDKATVYYDPSDPMGSNYVRGVIQRAWVKRVSEEMKKVVRDLQAAFRWMPAPQPPISRVPGNPFDFEAPVKGLKYFDFLVPGLVVLTATMGSIFGMGRVMMEEIETGVTYALFAAPIRPRDVVIGRFLNMSLWGAVRCGIVLVTALALGAKVPHPFLLLGVGILSTATMIGFALLMASLAGRSEVAEMLTGALTTPMMFLSGIFMPPSVMPEWAYEVARVNPMYYMGDAARKAALLGYLDPVDIAVLVVFATVFIATGAMLYDRIREHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU5</accession>
    <name>Q8TWU5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02149">
        <property type="protein sequence ID" value="AAM02149"/>
        <property type="gene ID" value="MK0936"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0936"/>
    <dbReference type="HOGENOM" id="CLU_2476022_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9714" checksum="C7BA587944321F53" modified="2002-06-01" version="1">MPERLYVRDYMVVGVAQVRMTDTVRDAVREMARAGVHGLAVVGLDGELVGVLEEEHIMDLVVERRGDWADILETPVEKVMNPEPAIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX21</accession>
    <name>TF2B_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIB</fullName>
            <shortName evidence="1">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfb</name>
        <name type="ordered locus">MK0858</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX21"/>
    <dbReference type="SMR" id="Q8TX21"/>
    <dbReference type="STRING" id="190192.MK0858"/>
    <dbReference type="EnsemblBacteria" id="AAM02071">
        <property type="protein sequence ID" value="AAM02071"/>
        <property type="gene ID" value="MK0858"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0858"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.901"/>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OMA" id="RTQKDFA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="PTHR11618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="SSF47954"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000119321" description="Transcription initiation factor IIB">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="123"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="218"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type" evidence="2">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="307" mass="34319" checksum="FFA72F58D108A075" modified="2002-06-01" version="1">MAASERNTGKFTEECPACGSAEIVFDEERGEYVCANCGLVTEDPVIDPGPEWRHFNPDQRQRRSRTGEPVKLRLPDKGISTIIDRELRDSGGKKNPRMRRIRTWDARIKVSGSRERNFFQAFLELENLASKLQLPESVRELAASIYRKAYKEGIVRGRGIESVLGAAVFAACKEARVPRTAREIAEALGVSDENEILRAYRVLQRRLNLKQKPTEPSDHLPRFASKLGVSENVQAKAQEIIEKAKEKGITVGKGPAGVAAAALYIASILEGERRTQKEIAEVARVTEVTIRNRYKEICEALGIELHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
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            <fullName evidence="4">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">MK0797</name>
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        <lineage>
            <taxon>Archaea</taxon>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="KEGG" id="mka:MK0797"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.838"/>
    <dbReference type="HOGENOM" id="CLU_600805_0_0_2"/>
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    <dbReference type="GO" id="GO:0051539">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
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        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
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        <property type="entry name" value="rSAM"/>
    </dbReference>
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        <property type="entry name" value="rSAM_horseshoe"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="160"/>
            <end position="388"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02011.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="435" mass="47999" checksum="A08237887DE4FEC6" modified="2002-06-01" version="1">MKVLVISPEYYTYGAIVVAGVCEEHGHSTILRRSPDPEALKRADVLILSLHTTLHLLDEDILEIARQAHEFGKPVIVGGPVSQVPELVLERFPNAIVAKGEAETGLPPLLQTLEDEGDFEDVEGIALLRDGEIVDTGWPPPADLDGPSPLKVPRDLGRQDVRGANVYIETHRGCPGACTFCQVPEFFGRRVRWKPVEAVLEEVRELTRGGARRFAISGGTVTTYGDDEEDFVELLKRLADLLGRENVSAPDVRADLLNERLLEAIRDYTIGWIFLGIESGSDRILRAMRKGITVDDVCEAVELARTVGVRVAGSFIVGYPGETEDDLEATEELLTELNLDDVFINLAEPIPGTELGRLVTELPEEEIPVLRPGEQLETEAGDRALQLQLTAFTTLSRPVPLTDDVFHEALHNIREDERKVLRITRFLRGAQGCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX81</accession>
    <name>Q8TX81_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">MK0794</name>
    </gene>
    <organism evidence="1 2">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="mka:MK0794"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.835"/>
    <dbReference type="HOGENOM" id="CLU_038447_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012032">
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    </dbReference>
    <dbReference type="Pfam" id="PF09890">
        <property type="entry name" value="DUF2117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006598">
        <property type="entry name" value="UCP006598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="395" mass="43647" checksum="32CD7EF5ED2603E5" modified="2002-06-01" version="1">MTSTSVVIVSREITKGVSEIRVGLVIHGPTVIDTGWAERILRSLKRLGEVRAKLGGTTGYVALLDAGLDSVVEFDRKLPSECLSELNEWADVLVLTNHGKSRESGLAFAEQVLSRAEGVDSLVQAERPGEPDGGIVLWNPGDAERTVAEHLREDLGLKITEVRTVRTEKGGGIGKSRRVACVEPGDRILVNGITVGVAESRNVELVFDDSGYLVEIRGGRIKPEGVERLGRVDPERVVVKTDRRLRRTEPERKRVKSGPERIHRVLLVDHDAERKVEDMRRSDAVVSVGDDTTCVCAELGDRLGVWVIGLVDLDPDGWVRDDTRESLRSSENLAALLVCERDDDAGKLVRGRFFRDREMRVLDPPVTVGELVEEVKECVKEVLKCVYHKAKETSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA2</accession>
    <name>Q8TXA2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0773</name>
    </gene>
    <organism evidence="1 2">
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            <taxon>Methanopyrus</taxon>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUF2118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09891">
        <property type="entry name" value="DUF2118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019115">
        <property type="entry name" value="UCP019115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="163" mass="18669" checksum="B6E4797EC3D5047C" modified="2002-06-01" version="1">MALPRIYVETDEVPESGKILLTDPEFEVIVRSRELAEKIAEELDEPIFYEQVVYEEARSVFDVPEAKVTRHVVIEPVDRRSLVFLKPGDRVYQIPVEGYVVTPIADVGDRLRKGDPLAAVTTRSGNVRYVEAPQDCLVVYVCEQPAIRTQRRPNYEYYIAPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB0</accession>
    <name>Q8TXB0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
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            <taxon>Methanopyrus</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01978">
        <property type="protein sequence ID" value="AAM01978"/>
        <property type="gene ID" value="MK0764"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0764"/>
    <dbReference type="HOGENOM" id="CLU_1100966_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="28293" checksum="E0BD7A0B183E9873" modified="2002-06-01" version="1">MKRRWKLYSRVVRELIAEGKNDRVAAALALEGYRRPPGGDRVHDDIVTLIKTERGLEGASLHPEKCLRLREILGLKLKEVWRRFQEPQARSAALDAVASAENVEPEEEISLPRDYGRAVTLRAKIVADTAEKLGDEVILVGYSAHIARELRDRGILLQIVDEDPRLRVERNIKFGESVLLSTGMVLSNGSVRRFLASHKGPVVLYSQSCPHLTAVLTRQGVVDAAVVEEFPYHFSPGGECKLRVYEPERPAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF1</accession>
    <name>ACDG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit gamma</fullName>
            <shortName evidence="1">ACDS complex subunit gamma</shortName>
            <ecNumber evidence="1">2.1.1.245</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ACDS complex methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhE</name>
        <name type="ordered locus">MK0723</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6,7,8-tetrahydrosarcinapterin + methyl-Co(III)-[corrinoid Fe-S protein] = 5-methyltetrahydrosarcinapterin + Co(I)-[corrinoid Fe-S protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45196"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59924"/>
            <dbReference type="ChEBI" id="CHEBI:64267"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.1.1.245"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>corrinoid</name>
            <dbReference type="ChEBI" id="CHEBI:33913"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <dbReference type="EC" id="2.1.1.245" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF1"/>
    <dbReference type="SMR" id="Q8TXF1"/>
    <dbReference type="STRING" id="190192.MK0723"/>
    <dbReference type="EnsemblBacteria" id="AAM01937">
        <property type="protein sequence ID" value="AAM01937"/>
        <property type="gene ID" value="MK0723"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0723"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.764"/>
    <dbReference type="HOGENOM" id="CLU_050002_0_0_2"/>
    <dbReference type="OMA" id="SCMAFAT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046356">
        <property type="term" value="P:acetyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01136">
        <property type="entry name" value="CdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016218">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023427">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000376">
        <property type="entry name" value="AcCoA_decarb_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155121" description="Acetyl-CoA decarbonylase/synthase complex subunit gamma">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00989"/>
        </source>
    </evidence>
    <sequence length="462" mass="50852" checksum="1E97B54407368D3B" modified="2002-06-01" version="1">MAQLSAMDVYNLLPKANCGACGCKTCMEFATKLVNREAKPEDCPKLDDESLEKLQELLAPPVKELTIGEGDREVTVGGDEVMFRHELSFFNPPPVFVTVYDDMEEDEIAGKTEEIQEFQVERVGEVLKLDGVAVVSRTGDPEKYARAVEIAVERSEDLAVALITTDPKVMEAGLDVFDERPLLYPATEENVEDLAKLAADGDCPLGLHARDVEDLVPLVVEAQQYTDDLLLDPGTEFGPHDVVSTTDKLAEIRKAAIEEFESFGYPTLVTTFPYAFLEDDPVKAARRESYLASACVLRYADILIMDTVEPWALLPVLTQRQCVYTDPREPQEVEPGLYRIGDPDENSPVLVTTNFTLTYHCVAGDLESADIDCWLLVIDTGGLAVDVSVAGGQFTGEAVKEVIEETDIEDKVEHRVLVIPGKAAAVKGDVEDATGWDVMIGTQDSSELPEFLEKEGLLRIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF4</accession>
    <name>ACDB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit beta</fullName>
            <shortName evidence="1">ACDS complex subunit beta</shortName>
            <ecNumber evidence="1">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhC</name>
        <name type="ordered locus">MK0720</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="1">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF4"/>
    <dbReference type="SMR" id="Q8TXF4"/>
    <dbReference type="STRING" id="190192.MK0720"/>
    <dbReference type="EnsemblBacteria" id="AAM01934">
        <property type="protein sequence ID" value="AAM01934"/>
        <property type="gene ID" value="MK0720"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0720"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.761"/>
    <dbReference type="HOGENOM" id="CLU_613408_0_0_2"/>
    <dbReference type="OMA" id="FMHLNQR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155103" description="Acetyl-CoA decarbonylase/synthase complex subunit beta">
        <location>
            <begin position="1"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="467" mass="53016" checksum="D8D30B08C2440AD3" modified="2002-06-01" version="1">MTSLADLPVDVSPRHEGERIRSGDMYVELAGPKSFGAELFKVVDPDEIEPDKVEVIGPDIDEMEEGGRYPFAIYVKAAGEELEEDVEGVLERRIHEFCNYVEGFMHLNQRDQIWCRVSKNVTEKGFRLEHLGIALRELYKEEFGNVIDSVEVTIMTDEEKVEEFLEYARRVYKKRDERAKGLSEEDVNEFYVCLMCQSFAPTHVCVITPDRPSLCGSITWHDAKAAYKIDPEGPIFPIEKGECLDPEAGEYEGVNEAVKEHSQGTVERVYLHSCLEYPHTSCGCFQAVVFYIPEVDGFGIVDREYPGETPIGLPFSTMAGEASGGEQQPGFVGVSYGYMESDKFLQYDGGWERVVWMPKALKERMKHAIPDELYDKIATEEDATTVEELREFLEKVEHPVVERWAEEEEEEEEKAPEEEAPAEEPTMEVKELPIAPGGGLNVKIVLKNAKIYAEKVIIKRADREDKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ5</accession>
    <name>TRPD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">MK0678</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ5"/>
    <dbReference type="SMR" id="Q8TXJ5"/>
    <dbReference type="STRING" id="190192.MK0678"/>
    <dbReference type="PRIDE" id="Q8TXJ5"/>
    <dbReference type="EnsemblBacteria" id="AAM01893">
        <property type="protein sequence ID" value="AAM01893"/>
        <property type="gene ID" value="MK0678"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0678"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.717"/>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154513" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="339" mass="36019" checksum="909A8DA1087BAAD2" modified="2002-06-01" version="1">MSVLERIIRGSDLDREEARDLMCRIVGGELSDVEVAGILVALRCKGYTSEELVGFVDGMMEHAVKVDPDVERLVDTAGTGGDELDTFNASTLAGLTAAAAGVPVAKHGNRSVTSECGSADILEALGVNIEADPDTVKRCIEEVGFGFMFAPKFHPAMKNVMPVRRKLGIRTVFNVLGPLTNPARERVTGQVIGVYSENLLDLVAGALAELGVRRGLVVYGLDGVDELSVTCENEVVYVDDGEVTDRDTVAPEDVGLDRADPKDVAGADPETSAEEARKILGGELPVDHPKVQMTAFNAGAALYVGEAVDSLEKGIQRALDVLEEGRALEVLEKVVDLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL9</accession>
    <name>Q8TXL9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01858">
        <property type="protein sequence ID" value="AAM01858"/>
        <property type="gene ID" value="MK0643"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0643"/>
    <dbReference type="HOGENOM" id="CLU_1881092_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="135" mass="15231" checksum="13C09712AA03BBE2" modified="2002-06-01" version="1">MEYPSIPRTLRLIMWAAYWINAVSVIATILLSRSRLNVPPEQTYSFLAIAVVFLAALGRALQRITGRRVVLTRDAVKFDGERIPYGDIEDVRVVEAGAVSVVEIRTTDGRVHRLRVADPDRFLRDLVGLLRFKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM3</accession>
    <name>Q8TXM3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01854">
        <property type="protein sequence ID" value="AAM01854"/>
        <property type="gene ID" value="MK0639"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0639"/>
    <dbReference type="HOGENOM" id="CLU_530644_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="513" mass="53704" checksum="149CD17CE5331287" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ1</accession>
    <name>Q8TXQ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing the ACT domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0609"/>
    <dbReference type="EnsemblBacteria" id="AAM01824">
        <property type="protein sequence ID" value="AAM01824"/>
        <property type="gene ID" value="MK0609"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0609"/>
    <dbReference type="HOGENOM" id="CLU_1264587_0_0_2"/>
    <dbReference type="OMA" id="YTQQFIK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040537">
        <property type="entry name" value="DUF5612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015832">
        <property type="entry name" value="UCP006363_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18462">
        <property type="entry name" value="DUF5612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006363">
        <property type="entry name" value="UCP006363_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="215" mass="22920" checksum="F5C3D4ABE0F7BC17" modified="2002-06-01" version="1">MLAISMDVENVPGALKAIAGVIADHGGNIVHIQQDSEGELARLYMEIEGVERPKVLVSELRSLDVVREVRVLRPLKEIYGKRVIVMGGGAQVAEVARGAITEADRHNIRGERISVDTIPLVGEENLAEAVRAVARLPRVGILVLAGSLMGGKIAEAVQELREEHGIPVISLNMAGSVPEVADLVVTDPVQAGVMAVMAISDAGVFDLSRVRGRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ2</accession>
    <name>Q8TXQ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein involved in ribosomal biogenesis, contains PUA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nip7</name>
        <name evidence="2" type="ordered locus">MK0608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0608"/>
    <dbReference type="EnsemblBacteria" id="AAM01823">
        <property type="protein sequence ID" value="AAM01823"/>
        <property type="gene ID" value="MK0608"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0608"/>
    <dbReference type="HOGENOM" id="CLU_114385_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0113" evidence="1">
        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="20466" checksum="880B793F78B69000" modified="2002-06-01" version="1">MKPELKRVPREELESIANEMARKFNCERVASVVRSFHLIKLEGRWTEYFVVNRVDFDPADLPERADPFLVGRPFCERSARGDLRIHVEGARELARRVDEIKTYVTERAAVLFTYGRDVLPESIVEIRGPKEPRLVAVVYRDDLGEHCVGVGRLRFSEDGRPVVDNVIDRGWYLRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR4</accession>
    <name>Q8TXR4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0596"/>
    <dbReference type="EnsemblBacteria" id="AAM01811">
        <property type="protein sequence ID" value="AAM01811"/>
        <property type="gene ID" value="MK0596"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0596"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.632"/>
    <dbReference type="HOGENOM" id="CLU_115699_0_0_2"/>
    <dbReference type="OMA" id="GCSDCRC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37460">
        <property type="entry name" value="PTHR37460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="154" mass="16777" checksum="FD27F24939284E4D" modified="2002-06-01" version="1">MLPDHGSYALVFRVNRRVKVNVGSLGDVIVEAGSWTYVGSGMGQGSSGLRGRLGRHLKSAREGTERPHWHIDHLLSQVRPEVEGAWIMEGDRECELAAALADMVPGIRGFGCSDCHCETHLFRAGIAETAEAVRRVGGGTFLPTPTLERWVSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS0</accession>
    <name>Q8TXS0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01805">
        <property type="protein sequence ID" value="AAM01805"/>
        <property type="gene ID" value="MK0590"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0590"/>
    <dbReference type="HOGENOM" id="CLU_1346412_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="203" mass="22729" checksum="494B8F31BA9363EE" modified="2002-06-01" version="1">MSSFKRLLYLDVISRSHADFAKRVIRRLEPKVKVRVECLSKYEREDDALTYDGRLKDEEPWKEFLRRLGRESSDTVVATALNVPPDVPDISPNPTVVIGVNTGDHPKTLEVAGLADVVVVGRRLYSPSTGPREFGRVRSGRLRNGTVIVDVEDLGLRYASKAVPVIAVLVAENGVDGLIRDLRRNRVRTLTEEVLRDLSLSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV4</accession>
    <name>Q8TXV4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA endonuclease related to intein-encoded endonucleases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01770">
        <property type="protein sequence ID" value="AAM01770"/>
        <property type="gene ID" value="MK0555"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0555"/>
    <dbReference type="HOGENOM" id="CLU_1881090_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00961">
        <property type="entry name" value="LAGLIDADG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAGLIDADG_2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="135" mass="15614" checksum="4FE6AC9EABA21215" modified="2002-06-01" version="1">MTFLTAGTRLVSLDYIAGFFDGEGSVVVRFVRDGRYRAGYRVSTKVVFVQKERDVLEEIHETLGMGHLYRRGSDGVWYLEIYRREDLREFVELIGNRTMVKRDALERLATVLELLEGGVHGSRDGLERIREVWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX2</accession>
    <name>Q8TXX2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Coenzyme F420-reducing hydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0537"/>
    <dbReference type="EnsemblBacteria" id="AAM01752">
        <property type="protein sequence ID" value="AAM01752"/>
        <property type="gene ID" value="MK0537"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0537"/>
    <dbReference type="HOGENOM" id="CLU_747252_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="370" mass="39748" checksum="198A3602C4618056" modified="2002-06-01" version="1">MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVAAERVCASCDASLGLAVVEAAEAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDVKKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPVDRVESVAKDAAEVADRLEDEVGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVVARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELGSGEGTAAVEAPEGTLIVSLRLRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRAIGLSGRCLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX4</accession>
    <name>Q8TXX4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01750">
        <property type="protein sequence ID" value="AAM01750"/>
        <property type="gene ID" value="MK0535"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0535"/>
    <dbReference type="HOGENOM" id="CLU_1811446_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="142" mass="16592" checksum="14925732E0949310" modified="2002-06-01" version="1">MHARCRCHTVKPTGANLRPLMKYIVAFRPGVDKRSLFRAVYLLDKAFERETGRPLTGTEYGHCMLLKAPYFSVDVKEAIFDLLRRGEIELRPERRNGELVSTYYPVGYRAEDIEDDVRREYSLHGDKWRLFREVLERVLGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY10</accession>
    <name>Q8TY10_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01711">
        <property type="protein sequence ID" value="AAM01711"/>
        <property type="gene ID" value="MK0496"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0496"/>
    <dbReference type="HOGENOM" id="CLU_1202631_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="230" mass="25848" checksum="0E869E5371F7C32B" modified="2002-06-01" version="1">MLRLPEFLVFIDHPSKKEVPDSEVEVARRRVEQLGGKIVWSIAGSIRKYHPDVRYVERLRKEGYELALHLSPGYIGLHRTSLATGVPEGKIKEAVYDALRIVDGAKYLTTCGPLHTALFRGKVPRRGWKDDLGPELHPSVVYGLGQAAAETGLDLLVGARSPEWIKGILDELEVKYIEGIGIDNYTIDDEKPEEAAKMVAEGECSAVIVHPRKDTFRRLHKFITCLKRIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY26</accession>
    <name>Q8TY26_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01695">
        <property type="protein sequence ID" value="AAM01695"/>
        <property type="gene ID" value="MK0480"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0480"/>
    <dbReference type="HOGENOM" id="CLU_1369537_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="199" mass="23506" checksum="8F5A1FA720750C57" modified="2002-06-01" version="1">MRSGIRKFRVTHKTFMTRKMDDEMAVISFVDDRHYDTYKEYMERLKELEVSCAVGWVNSLGCEAFFPKSVESILENWHSVEAASQMIHAGVTDVVLLEHNYSKYLFREFHTKFRRSWEKEGISYRAPLGELYPDYVNEIILAHNFLRSLSMDVRGMINVSLHRDRMVVKTSRRECSVWYEDIREDAMETMQAVERALVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY28</accession>
    <name>Q8TY28_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase, MoxR-like family of the AAA+ class</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0478"/>
    <dbReference type="EnsemblBacteria" id="AAM01693">
        <property type="protein sequence ID" value="AAM01693"/>
        <property type="gene ID" value="MK0478"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0478"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.508"/>
    <dbReference type="HOGENOM" id="CLU_775256_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="74"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="357" mass="40361" checksum="11B45382DCA9AE82" modified="2002-06-01" version="1">MGGSRSGGFILSKIIMRPQVTIEDDWIIFEGEEGFVRLPKDPYKVIEDFEEYGYFFDEEHRRDFAAMYMTLIVEQTPVLLVGPPGTGKTKLVRLIGELYDVPVVVMRGHQEAREQEFIGGVDVAALPVADKIAEEYTEVGNMEYEEAIEALKEFIYVGGYLKDVVNDARRRGACLFFIDEVNRFPGYMIPTLIHIFEKDTEVIYIPHSPIDFGETGAIMRIGALNPEGKGTTNFDEALLRRVELVPWGEYDVEIYKRIAIESARQIIELTKMAEEVLEDLASLIKDMGKGVDVAKDVAQTVAIGIKMGYDVDTVARNVANKILGDMLLDSEKAEEFEARRNALERKIKEKLKPVFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY30</accession>
    <name>Q8TY30_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ehaB</name>
        <name evidence="1" type="ordered locus">MK0476</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0476"/>
    <dbReference type="EnsemblBacteria" id="AAM01691">
        <property type="protein sequence ID" value="AAM01691"/>
        <property type="gene ID" value="MK0476"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0476"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.506"/>
    <dbReference type="HOGENOM" id="CLU_1623471_0_0_2"/>
    <dbReference type="OMA" id="FMGNIVC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011314">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaB"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019706">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="17790" checksum="E1DE9C3D7C4D8D6E" modified="2002-06-01" version="1">MIGLQEGLQILQIVVGSVLAWLNFVIVDILMGLPEAPGVKGAEAIGRSVERRGGDIAGGYFMGNIVCSPDASAGTLLASSGYYCLGGPEGGLVAALAVYLGNRLCADPGYAGTLGSLTATVLVTLYDKVLGMDPANFVAGMVIAIMTIHGIDHPRASRLIGDIARRMGRGAEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY77</accession>
    <name>CFBA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="1">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CbiXS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sirohydrochlorin nickelchelatase</fullName>
            <ecNumber evidence="1">4.99.1.11</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiX</name>
        <name evidence="1" type="synonym">cfbA</name>
        <name type="ordered locus">MK0428</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:52796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="EC" id="4.99.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="1"/>
    <dbReference type="EC" id="4.99.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY77"/>
    <dbReference type="SMR" id="Q8TY77"/>
    <dbReference type="STRING" id="190192.MK0428"/>
    <dbReference type="EnsemblBacteria" id="AAM01643">
        <property type="protein sequence ID" value="AAM01643"/>
        <property type="gene ID" value="MK0428"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0428"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.457"/>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="OMA" id="HEHEKLE"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150357" description="Sirohydrochlorin cobaltochelatase">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <sequence length="143" mass="16083" checksum="E18D2709A9CD1138" modified="2002-06-01" version="1">MVAVVLVGHGSRLPYSRQVVEKIAEYVEEMGDFETVEVGFMELCEPTVQEAVKKAAESGVDKIVVVPVFLAHGVHTKRDIPKMLGLEPEWDDDEDDHDHHHHHHRDYTPVDVDAEIVYAEPLGADPRIAEIVIDRIKEALGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY91</accession>
    <name>RL4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl4</name>
        <name type="ordered locus">MK0414</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY91"/>
    <dbReference type="SMR" id="Q8TY91"/>
    <dbReference type="STRING" id="190192.MK0414"/>
    <dbReference type="EnsemblBacteria" id="AAM01629">
        <property type="protein sequence ID" value="AAM01629"/>
        <property type="gene ID" value="MK0414"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0414"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.443"/>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="OMA" id="WHQKVNV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_L4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_L4/L1e_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_L4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_L4-archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="PTHR19431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129333" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <sequence length="260" mass="28710" checksum="EDA6E36D701FAE11" modified="2002-06-01" version="1">MEAPVFNLEGEEVDTVELPSFFEEPVRKDLIRRAVLAAQANRRQPYGTDPRAGFRTSAESWGAGHGVAMVPRVKGRRHPAAGRAARVAQAVGGQKAHAPTPEKDWTQRVNRKERRAALRSALAATAKPEFVKERGHVIDDVPHLPVVVVDELKSLNKAREVREFFKSVGLWADVERAKSNRRIRAGKGKRRGRRYVKPKSVLIVVDEDEGIKLGARNHPGVDVVEAMHLGVEHLAPGAHPGRLTVFTPGALEVLEERLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY97</accession>
    <name>Q8TY97_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized membrane protein/domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0408"/>
    <dbReference type="EnsemblBacteria" id="AAM01623">
        <property type="protein sequence ID" value="AAM01623"/>
        <property type="gene ID" value="MK0408"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0408"/>
    <dbReference type="HOGENOM" id="CLU_1965566_0_0_2"/>
    <dbReference type="OMA" id="IGWIAMP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010432">
        <property type="entry name" value="RDD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06271">
        <property type="entry name" value="RDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="RDD" evidence="6">
        <location>
            <begin position="9"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="127" mass="13816" checksum="AD7D969656154E33" modified="2002-06-01" version="1">MNPAPSELRFGAYAIDCVITAVMSLLICLALARPVTPVRFLSTWSLISWIYWTMFEGTYGESPGKRVFGLKVVNEEGEAVSLPEAAIRNVSKALPVVCYVDGALILLTESRQRAFDLLAGTFVVTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD8</accession>
    <name>Q8TYD8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0362</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01577">
        <property type="protein sequence ID" value="AAM01577"/>
        <property type="gene ID" value="MK0362"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0362"/>
    <dbReference type="HOGENOM" id="CLU_835795_0_0_2"/>
    <dbReference type="OMA" id="WGCCIAY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="331" mass="36205" checksum="457CAB0B2AAEF7F5" modified="2002-06-01" version="1">MEGIRNQIERVLDPREGACGIAHATLEVLSWSGYRVECLEERLGVRARLIGPDGSVTEGRDVTWAPAILESLIKSGVYPEGWEERLSEVLTPERDMRRLARVFGYGRVLTVDRVAARIILGGGGTVIVRRRGLGSEVEIRYDGSKSDYVSYCPACALALAAVRHPQVYRELKRELADAPNTGKVKAEDGVVNSVRVRRGIAFATLKLANRSITNRGCCVAYAIVRAELKAGYGSERSKRLLRAYCDECPLKHCWVGKPISALGNVVLQRLTETEGGVRLKVEEYPEVVTPAGTGRGTLCALSACANAVLRLDASKVLKPDPSRSEAWGDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE1</accession>
    <name>Q8TYE1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Asp-tRNAAsn/Glu-tRNAGln amidotransferase C subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gatC</name>
        <name evidence="2" type="ordered locus">MK0359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01574">
        <property type="protein sequence ID" value="AAM01574"/>
        <property type="gene ID" value="MK0359"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0359"/>
    <dbReference type="HOGENOM" id="CLU_196465_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010120">
        <property type="entry name" value="Glu-ADT_subunit_C_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01827">
        <property type="entry name" value="gatC_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="84" mass="10024" checksum="9FE4D635141E0D93" modified="2002-06-01" version="1">MASDRDDVDVLDILRETMELLEEFAERLEDVDAEDETWTTSEGSRKFREDDEPRCDREFKKKMLENAPRSDDGYVIAERAHWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE7</accession>
    <name>Q8TYE7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain conserved in archaea fused to a metal-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0353</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0353"/>
    <dbReference type="EnsemblBacteria" id="AAM01568">
        <property type="protein sequence ID" value="AAM01568"/>
        <property type="gene ID" value="MK0353"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0353"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.374"/>
    <dbReference type="HOGENOM" id="CLU_1154327_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018664">
        <property type="entry name" value="DUF2103_metal-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09876">
        <property type="entry name" value="DUF2103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26286" checksum="80E6007DC01B4E5D" modified="2002-06-01" version="1">MGNVPRCRRCGYSVELPLKCPRCGEPSFEVAAFQVYPEDVRYVVAFVNESDVHTIHQSFQGDPDGIMLRNLVERLGELLHSAAPRAATAVRTPPWIVDVVERISGVTVRTVRDDFDDVVRSLQAELRADRRLDRVEEPPERKLGGSHSTIIGGRRGRELVLKVASVPYVKRVIPGRIGAKGSRGGGGVRLKVSRVDDSGNVKLLLSEGAATQEIFVVTTARDEREGKLVAELLERVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG1</accession>
    <name>Q8TYG1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine synthetase</fullName>
            <ecNumber evidence="2">6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glutamate--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glnA</name>
        <name evidence="7" type="ordered locus">MK0338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0338"/>
    <dbReference type="EnsemblBacteria" id="AAM01553">
        <property type="protein sequence ID" value="AAM01553"/>
        <property type="gene ID" value="MK0338"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0338"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.359"/>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="OMA" id="TFYTHPE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gln-synt_C" evidence="6">
        <location>
            <begin position="151"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-AMP-tyrosine" evidence="4">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="491" mass="56366" checksum="79AD92D8E110CF11" modified="2002-06-01" version="1">MPRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVESKDVKFVRLQFVDIHGFVKNFAIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDPDTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKRVMNELAEKGYMMYCGPECEFYLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHEIDFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGKENLFYDPDAEDQYYLSDTALYYIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWSPKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAVAGVDGIENKIDPGDPVNENLYEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERVTKWEIKKFIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH2</accession>
    <name>Q8TYH2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01542">
        <property type="protein sequence ID" value="AAM01542"/>
        <property type="gene ID" value="MK0327"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0327"/>
    <dbReference type="HOGENOM" id="CLU_1014152_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="274" mass="30881" checksum="E4EF3AC2AE8B75CD" modified="2002-06-01" version="1">MPLALIYIFFASVVLWPISVAIIAVKWRRARKILNEAIGSGTVGPYGGVNGETGVRLSSTKVVWYYGIFDFPPIGLPVVALLSAVALGKGWENLTACPGIRNLSMSLLTWVFVNSCLLGIVGKLVWYLRLKRLDVKFIAESKKKVNRNIIKKICEKYKYIDVYSAFNVTLMMWFYNIFAGGIIVSLFTLIFYLTNTKTLLIHRYYYNILTVSISLVILTNSVLIGLWFSRRKYICSPKIQSNSIIIYLITAIVTIYTSVISCAMFEYVITGSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH3</accession>
    <name>Q8TYH3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01541">
        <property type="protein sequence ID" value="AAM01541"/>
        <property type="gene ID" value="MK0326"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0326"/>
    <dbReference type="HOGENOM" id="CLU_481140_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="566" mass="62369" checksum="71972A64F30BE2DE" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ3</accession>
    <name>Q8TYJ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01521">
        <property type="protein sequence ID" value="AAM01521"/>
        <property type="gene ID" value="MK0304"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0304"/>
    <dbReference type="HOGENOM" id="CLU_1363651_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="200" mass="23276" checksum="DFCF09851EB2085B" modified="2002-06-01" version="1">MEITHGRPYDEGSGWHEIFIDPTGPMMHKPWKIRDSVVLVPKHKPMNDDEYACASSQRPYDMLSEAVRDAISRFVSPETMERLKIAWDGLKGLSIGLMMERFIYTNFLTAQGVSKLIDKLFFGGNLEFEIGNWFDRVRRFPGWDASEDGVERRWRAFKSEWARWRVKEREPKKSPTIWVIILAGSLAPLAWFRGARGVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK9</accession>
    <name>Q8TYK9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metal-sulfur cluster biosynthetic enzyme (MinD N-terminal domain family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaD</name>
        <name evidence="2" type="ordered locus">MK0287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0287"/>
    <dbReference type="EnsemblBacteria" id="AAM01504">
        <property type="protein sequence ID" value="AAM01504"/>
        <property type="gene ID" value="MK0287"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0287"/>
    <dbReference type="HOGENOM" id="CLU_091588_3_0_2"/>
    <dbReference type="OMA" id="TVADPHM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeS_assembly_P" evidence="1">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9701" checksum="3D03FE75B66B2344" modified="2002-06-01" version="1">MPSEEEVLKELKKVKDPHTGLDIVSMRLVEEVNADEENIEVVVRPTNPFCPSALMIVEQVKATLESAFEGVNVDVKLVGHVLTEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN3</accession>
    <name>Q8TYN3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted transporter component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0262</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0262"/>
    <dbReference type="EnsemblBacteria" id="AAM01479">
        <property type="protein sequence ID" value="AAM01479"/>
        <property type="gene ID" value="MK0262"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0262"/>
    <dbReference type="HOGENOM" id="CLU_1673998_0_0_2"/>
    <dbReference type="OMA" id="CFCGGIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="7">
        <location>
            <begin position="23"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="16484" checksum="108873C41C91C040" modified="2002-06-01" version="1">MAEYLHAIGTLAFGVLIGYLGQRSAMCFIGGIRDVYLLRDTWLVQGLIGFLIGAFFGLVVFGAAGMIKKFPWFLYKGASAIPGDVLGKKPGFAAHAAVTVIGGLGVGFLSVVQGGCPFRNYVMAAEGNVTAMAYLLGVFVGAVFFHACIVPIFGPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR0</accession>
    <name>Q8TYR0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metal-dependent hydrolase of the TIM-barrel fold</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01449">
        <property type="protein sequence ID" value="AAM01449"/>
        <property type="gene ID" value="MK0232"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0232"/>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="OMA" id="ETLMDHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="251" mass="27577" checksum="BF3ED9C06BC62102" modified="2002-06-01" version="1">MFVKFDSHVHLDVRCEDDMKTMSLAGIRYVLTLAHDPMPFRTAEALLGHWEAVESTAETAVDYLIDAKVGLGVHPRAIPDEGLELALEHLESKLSDLDAVGEIGLEEATDEEVKVFREQLDLAATEDVPVVVHTPRSRDPNVISKIIEVARDSDLHPDLIVIDHLNEQYVDAVLAEGFNAGITVQPGKATVEEAVEIVTNRAEHADRILINSDASRALSNVLAVAEVAFELEKRGFDATEAVVRDNALQLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS9</accession>
    <name>Y213_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein MK0213</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0213</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYS9"/>
    <dbReference type="STRING" id="190192.MK0213"/>
    <dbReference type="EnsemblBacteria" id="AAM01430">
        <property type="protein sequence ID" value="AAM01430"/>
        <property type="gene ID" value="MK0213"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0213"/>
    <dbReference type="HOGENOM" id="CLU_079268_0_0_2"/>
    <dbReference type="OMA" id="YHYDHHT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="PTHR43546:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057632" description="UPF0282 protein MK0213">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <sequence length="293" mass="33216" checksum="6860D9A617A748B7" modified="2002-06-01" version="1">MRVVPLGFESLGVRSMATLIETPDVTVLVDPGVSIPPKRYNLPPSEEEWEALEEVRESIQRAADSADVVTISHYHYDHHTPFTDRKYEACDLETAKELYRDKLILMKHPTENINRSQAGRARALIEGLDELGVDYEFADGKRFEFGETVLEFSQPLPHGPEGTRLGYVLGLRITHRDHVIVHASDVQGPVYGPALEWILERDPDLVLISGPPTYLLGFRFSSDNLEKAVKNLRKLASRSGQIILDHHLLRDKNYRDRLSEVYEESDNVASAAEVLGKEERLLEAYRDELSGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-25" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT5</accession>
    <name>ENGB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">MK0207</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYT5"/>
    <dbReference type="SMR" id="Q8TYT5"/>
    <dbReference type="STRING" id="190192.MK0207"/>
    <dbReference type="EnsemblBacteria" id="AAM01424">
        <property type="protein sequence ID" value="AAM01424"/>
        <property type="gene ID" value="MK0207"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0207"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.208"/>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_2"/>
    <dbReference type="OMA" id="PNHYDWA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000157811" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
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        <location>
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            <end position="190"/>
        </location>
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        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="203" mass="23200" checksum="6268DE55BC2DFE5A" modified="2002-06-01" version="1">MPEIVLVGRSNVGKSSLIRAITRGAADVRVGKRPGVTRKPVFHELDGELVLVDMPGFGFMSGVPRRYQERVKDLIVRYLEEKDNILFAIHVVDAKALPEIAERWERRGEIPIDREMFQFLNEVGLDPIVAANKIDKIKPIEFEEHMDAVAEALGLFPPWRQWLDTLFPISAKTGEGLVEFLEALQERVRKAGYPEFARFFRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
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    <name>Q8TYU9_METKA</name>
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        <submittedName>
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        </submittedName>
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            <taxon>Methanopyraceae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="KEGG" id="mka:MK0192"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.193"/>
    <dbReference type="HOGENOM" id="CLU_345358_0_0_2"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATPase_AAA-3"/>
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        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
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    </dbReference>
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    </dbReference>
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            <end position="816"/>
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            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="AAM01409.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="818" mass="91451" checksum="8FE658F4E241B7D2" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ09</accession>
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        </submittedName>
    </protein>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_023081_2_0_2"/>
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        <property type="component" value="Chromosome"/>
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    </dbReference>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="175"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="492" mass="55721" checksum="4E10FA3C12308618" modified="2002-06-01" version="1">MVRDLNEAVVIRIETPERSERVKVPYREGMTLLDALRWIKEHEIPDLEFEFSCRNAQCGTCAVLVNGKARLACEYRLEPGQEVTVGPLRHLPVVKDLAVDWSAVTSRLRPLSPKSHREWFRRMEPEKQRKLYELRSCIECLCCVAECPVIKAGSARNPGPIVLRKVAEEAEKWDESPEIDDSVYACTTCHTCAEVCPKDIEIPAKAVETVRARLYEEGKGPLPEHKELGERAVRTGRSVEKRDRSFLEEYSGEYGEGDVKAMFFTGCLVDYRLPDTGKALVRLAEELGIRLIVPREQVCCGSPLLRTGQHDRAERLAFENLETFRRVDPDVIVTVCAGCGATLKNNYPELLGDRFEWDVLDVTELLVEIRAHERGFRLPERTTVTYHDPCHLKRGQGVEDEPRKLIRSIENVEFVEMEEPDRCCGAGGGVRSGLPELAELMSDVKAHMVRETGAEVLTTVCPFCEYNLREGLERNDVEARVENLTVLLSRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ31</accession>
    <name>Q8TZ31_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0108"/>
    <dbReference type="EnsemblBacteria" id="AAM01325">
        <property type="protein sequence ID" value="AAM01325"/>
        <property type="gene ID" value="MK0108"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0108"/>
    <dbReference type="HOGENOM" id="CLU_1163804_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26676" checksum="7F0B9E1BCDB7DBC2" modified="2002-06-01" version="1">MAKAERSDAGKDKVWIPDEEAKRIGADVSLQVLDALAKRDYPVYDLKKHAELDVRSVAREVVREIGGMAGLEFSLKGVDTVLDKFLSKIPVVKTVVAEEELEPVAEEDRYVCFIAVDPRNYRELREKAGAIAVTAREELDLDHTPLVLVRNDLCVGPEEFGVLALHSSEVAKERPPFKVEVLVGEDLKPTMIRLARFIASTVATRTVYLKFPYVVVKESLLVEKGDYKVRVKTPEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ49</accession>
    <name>Q8TZ49_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01307">
        <property type="protein sequence ID" value="AAM01307"/>
        <property type="gene ID" value="MK0090"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0090"/>
    <dbReference type="HOGENOM" id="CLU_2420054_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="91" mass="9956" checksum="7C8E7C0EDC942F34" modified="2002-06-01" version="1">MRVGDLSGEPEYKLALAAVHQGLGILAGYLCATGYSIVLVMALLILPFALGKVLERLLERERIGGAKGWLAYGLGAYLSAWILVWTYFINR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ54</accession>
    <name>Q8TZ54_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fumarate hydratase class I, N-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ttdA</name>
        <name evidence="8" type="ordered locus">MK0085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0085"/>
    <dbReference type="EnsemblBacteria" id="AAM01302">
        <property type="protein sequence ID" value="AAM01302"/>
        <property type="gene ID" value="MK0085"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0085"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.85"/>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_2"/>
    <dbReference type="OMA" id="IETYPTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="7">
        <location>
            <begin position="15"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="303" mass="33265" checksum="70F257AD094DB182" modified="2002-06-01" version="1">MPDDSDRELLESIAEATVEALRVAETKLPDDVLERVERALEEEEDDHARMMLEAILENVRIAEEKGLPMCQDTGLITVFAEIGREFPLRLAGTIRDGIEEGIRRATEEIPLRPNVVHPISRENTGDNTGDRVPIVRFLPTKGEELRLHFLPKGFGSENSSAVTRLLPTEGLEGVREFVIKTVREAGGMPCPPIVLGVGVGGTIDEAAHLAKLALFRPLNVRNPDPEIAKLEEELLEEINRLGVGPMGLGGRTTALAVNVELAYTHTAGLPVAVNVQCWAARRATAIVYPDGFFEVTQREYPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ64</accession>
    <name>Q8TZ64_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01292">
        <property type="protein sequence ID" value="AAM01292"/>
        <property type="gene ID" value="MK0075"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0075"/>
    <dbReference type="HOGENOM" id="CLU_893151_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="311" mass="33773" checksum="10C015023A700CEE" modified="2002-06-01" version="1">MWVELRRKYGRDRGHAPMSEEDVLRVKPKERPGVVFVTPDGRVHADLGHVRMVEDTVTLHSDPTEPPGPDNPSVIVPEHVLAALYVSGYAGAVVEIRGDPGHAEHVWRADCYLREAVEELGGPADPPEMGEPAAKRAFIREGEAPSTVRNVEGRIEPAEEFEVRVGGEEVSVHDLMTVRRGNVSNKTPEHAGLDVVADVAMLAGTACARVEISRPTEVTSIHPTDLYLATAAKHGIRVCSLNVGRMRGAVNLEEGETVRVGTDLRYEVCITEEPVASEIKIGDVTLVREGGKFRVVEPGTVRLGDEVVEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB8</accession>
    <name>Q8TZB8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea, FLPA ortholog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01234">
        <property type="protein sequence ID" value="AAM01234"/>
        <property type="gene ID" value="MK0017"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0017"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.16"/>
    <dbReference type="HOGENOM" id="CLU_539284_0_0_2"/>
    <dbReference type="OMA" id="HHGYMCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016760">
        <property type="entry name" value="MonoFe_hyd_HmdC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10113">
        <property type="entry name" value="Fibrillarin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019375">
        <property type="entry name" value="UCP019375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03958">
        <property type="entry name" value="monoFe_hyd_HmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="511" mass="55098" checksum="ABD74A4997C7C96E" modified="2002-06-01" version="1">MLDLLHEACESLDAALELKRLAYEGKLSVDEVVTDLGELTDRELEALGDNLRTFPMGCDLIELAVGPCASDYSPDVLLANAILADRMGLPLHVCAYAVADVAENYGMRPIELFRRLVRNVRVPVDVDHIGAHGPMRFPRDITACEGTCYLEGPPFKECPRGRIHRRLIDKERQQGEDLEEWAELAASICVNVTGEGGQDAEAHAAPLDEMKRVAETARHSGAGVGAILHVADGEDEFADGLKAAVEEVKADYLAVEGGPFNRAEDRLSAFRRAVVACRVFAPGKVVLTNGAYEDELVVGLRAGLNGALSGFPKNHHGYMVGYEPGTARRGKFGLPKVLAIMRKTLGSRYGNTKVPAGWEELEGITRAALFLGSNLLYPRDVAGIPIGDVHWVAALRSNAARELSDEVRSVEEVASEVDADTVALLGGRFPAWGLALTLDELGVSEVLISDPDAWVERATVRLLDEELDATVHAMAGDDRKAVKEADAAFVTAVMPGIAERLAERTGAMTVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB9</accession>
    <name>Q8TZB9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted enzyme related to biotin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK0016</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0016"/>
    <dbReference type="EnsemblBacteria" id="AAM01233">
        <property type="protein sequence ID" value="AAM01233"/>
        <property type="gene ID" value="MK0016"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0016"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.15"/>
    <dbReference type="HOGENOM" id="CLU_033172_0_0_2"/>
    <dbReference type="OMA" id="IMGFNPY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023858">
        <property type="entry name" value="RSAM_HmdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00330">
        <property type="entry name" value="HMD_cofactor_maturase_(HmdB-li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03957">
        <property type="entry name" value="rSAM_HmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="50"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="39362" checksum="36A0E7C91BF483F5" modified="2002-06-01" version="1">MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHVTNECRIRPRCAYCGFAAGASPEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDGGKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDVETICCNLETTNRELFERLKPGDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPYPGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIPDNHPHEVKGVGNPRTGNLDRVVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-04-03" modified="2022-08-03" version="147" xmlns="http://uniprot.org/uniprot">
    <accession>Q9UXR8</accession>
    <name>HEM1_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Glutamyl-tRNA reductase</fullName>
            <shortName>GluTR</shortName>
            <ecNumber>1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hemA</name>
        <name type="ordered locus">MK0200</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Biol. Chem." volume="274" first="30679" last="30685">
            <title>Methanopyrus kandleri glutamyl-tRNA reductase.</title>
            <authorList>
                <person name="Moser J."/>
                <person name="Lorenz S."/>
                <person name="Hubschwerlen C."/>
                <person name="Rompf A."/>
                <person name="Jahn D."/>
            </authorList>
            <dbReference type="PubMed" id="10521455"/>
            <dbReference type="DOI" id="10.1074/jbc.274.43.30679"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 1-30</scope>
        <scope>FUNCTION</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>CATALYTIC MECHANISM</scope>
        <scope>MUTAGENESIS OF CYS-48 AND HIS-84</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="EMBO J." volume="20" first="6583" last="6590">
            <title>V-shaped structure of glutamyl-tRNA reductase, the first enzyme of tRNA-dependent tetrapyrrole biosynthesis.</title>
            <authorList>
                <person name="Moser J."/>
                <person name="Schubert W.-D."/>
                <person name="Beier V."/>
                <person name="Bringemeier I."/>
                <person name="Jahn D."/>
                <person name="Heinz D.W."/>
            </authorList>
            <dbReference type="PubMed" id="11726494"/>
            <dbReference type="DOI" id="10.1093/emboj/20.23.6583"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG</scope>
        <scope>SUBUNIT</scope>
        <scope>CATALYTIC MECHANISM</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA). In the absence of NADPH, exhibits substrate esterase activity, leading to the release of glutamate from tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by heavy metal compounds, Zn(2+), and heme. Also competitively inhibited by glutamycin.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 8.1.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="mass spectrometry" mass="45436.0" error="30.0" method="Electrospray" evidence="1"/>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70"/>
    <dbReference type="EMBL" id="AJ131561">
        <property type="protein sequence ID" value="CAB59204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T45026">
        <property type="entry name" value="T45026"/>
    </dbReference>
    <dbReference type="PDB" id="1GPJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-404"/>
    </dbReference>
    <dbReference type="PDBsum" id="1GPJ"/>
    <dbReference type="AlphaFoldDB" id="Q9UXR8"/>
    <dbReference type="SMR" id="Q9UXR8"/>
    <dbReference type="STRING" id="190192.MK0200"/>
    <dbReference type="EnsemblBacteria" id="AAM01417">
        <property type="protein sequence ID" value="AAM01417"/>
        <property type="gene ID" value="MK0200"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0200"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.201"/>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="OMA" id="FAFKCAA"/>
    <dbReference type="BRENDA" id="1.2.1.70">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q9UXR8"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114102" description="Glutamyl-tRNA reductase">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="174"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of activity." evidence="1">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Complete loss of activity." evidence="1">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="30% of wild-type reductase activity. 15% of wild-type esterase activity." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="4"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="11"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="40"/>
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        </location>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
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                <person name="Malykh A.G."/>
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        <citation type="journal article" date="2013" name="Nucleic Acids Res." volume="41" first="657" last="666">
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            <dbReference type="DOI" id="10.1093/nar/gks1017"/>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="4GFJ"/>
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</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>O32869</accession>
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    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
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            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
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    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step. MtrH catalyzes the transfer of the methyl group from methyl-tetrahydromethanopterin to the corrinoid prosthetic group of MtrA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32869"/>
    <dbReference type="SMR" id="O32869"/>
    <dbReference type="STRING" id="190192.MK0662"/>
    <dbReference type="EnsemblBacteria" id="AAM01877">
        <property type="protein sequence ID" value="AAM01877"/>
        <property type="gene ID" value="MK0662"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0662"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.701"/>
    <dbReference type="HOGENOM" id="CLU_048697_0_0_2"/>
    <dbReference type="OMA" id="YARHKIV"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01501">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023467">
        <property type="entry name" value="MeTrfase_MtrH/MtxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028342">
        <property type="entry name" value="MtrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02007">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500206">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004960">
        <property type="entry name" value="MtrH_MtxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01114">
        <property type="entry name" value="mtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147564" description="Tetrahydromethanopterin S-methyltransferase subunit H">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1." evidence="2" ref="1">
        <original>MFYY</original>
        <variation>MDRCSTT</variation>
        <location>
            <begin position="1"/>
            <end position="4"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA74773." evidence="2" ref="1">
        <original>A</original>
        <variation>G</variation>
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="322" mass="34963" checksum="40661B810E6406EC" modified="2002-04-16" version="2">MFYYPGKEQKVCDICGVKVGGQPGEYPTVLAGTIFYAGHKIVKDEDKGIFDEEAAEELIKMEEELADETGNPMMAHIMGESEEAIIRYLDWVADVTDAPIIVDSTEAEVKVAAVKHAQEVGLAERVVYNSINASVEDEEIQAIKESDCNSAIVLAFNPMDASVEGRMKILTEGEEGVSEKGMLEISDECGIENPLIDTAYTPFGSGAGTAYKVTLAVKAKLGLPVGGAPHNVPSAWDWLRDFMKKLKEEGKEEWAELAHESSDWGSNVVAATLCCDYLLFGPIENAPAIWPVVAMVDALIVEANEDVGVEPQVEEHPANIVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-08-03" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>P0C1V6</accession>
    <name>VHUU_METKA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu subunit U</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuU</name>
        <name type="ordered locus">MK0177.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The large subunit of Vhu is split into VhuA and VhuU. Each contributes two ligands to the [NiFeSe] center.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000249594" description="F420-non-reducing hydrogenase vhu subunit U">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000249595" description="Removed in mature form" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="42" mass="5092" checksum="6D0614F1CD624BF1" modified="2008-02-26" version="2" precursor="true">MAEKRDVLNYLEMVIRSYDIULSCAAHVLDRVKFRIERKDED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>P50016</accession>
    <accession>Q49607</accession>
    <name>THS_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Thermosome subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Chaperonin-like complex</fullName>
            <shortName>CLIC</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ths</name>
        <name type="ordered locus">MK1006</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="FEBS Lett." volume="379" first="127" last="131">
            <title>Purification and structural characterization of the thermosome from the hyperthermophilic archaeum Methanopyrus kandleri.</title>
            <authorList>
                <person name="Andrae S."/>
                <person name="Frey G."/>
                <person name="Nitsch M."/>
                <person name="Baumeister W."/>
                <person name="Stetter K.O."/>
            </authorList>
            <dbReference type="PubMed" id="8635576"/>
            <dbReference type="DOI" id="10.1016/0014-5793(95)01493-4"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an oligomeric complex of eight-membered rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="Z50745">
        <property type="protein sequence ID" value="CAA90621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Z49052">
        <property type="protein sequence ID" value="CAA88843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S54118">
        <property type="entry name" value="S54118"/>
    </dbReference>
    <dbReference type="PIR" id="S68687">
        <property type="entry name" value="S68687"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P50016"/>
    <dbReference type="SMR" id="P50016"/>
    <dbReference type="STRING" id="190192.MK1006"/>
    <dbReference type="EnsemblBacteria" id="AAM02219">
        <property type="protein sequence ID" value="AAM02219"/>
        <property type="gene ID" value="MK1006"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1006"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1055"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="PTHR11353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128391" description="Thermosome subunit">
        <location>
            <begin position="1"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="545" mass="59474" checksum="775A89D3312AAF17" modified="1996-10-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>P58829</accession>
    <name>Y399_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein MK0399</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0399</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58829"/>
    <dbReference type="SMR" id="P58829"/>
    <dbReference type="STRING" id="190192.MK0399"/>
    <dbReference type="EnsemblBacteria" id="AAM01614">
        <property type="protein sequence ID" value="AAM01614"/>
        <property type="gene ID" value="MK0399"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0399"/>
    <dbReference type="HOGENOM" id="CLU_134829_1_0_2"/>
    <dbReference type="OMA" id="VARSKCI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="PTHR39652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094511" description="UPF0201 protein MK0399">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <sequence length="159" mass="18195" checksum="03D02F7D60CECDA9" modified="2002-05-27" version="1">MISRVVLTTYVYPTEDEEKVRKAVGNLFDLEMFEEREEEMGDLRRLEFVCEGPQARLSLGRIYELLREQEILDAARRVLREGVTAEGSILFHLNKQAAFAGSVSFAEGGESPLGPIVVEVFPERPEDVEKVIDWLAPETIDGKPIYEVKKPRLREEELE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-24" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>P58879</accession>
    <name>HIS7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="1">IGPD</shortName>
            <ecNumber evidence="1">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisB</name>
        <name type="ordered locus">MK0855</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58879"/>
    <dbReference type="SMR" id="P58879"/>
    <dbReference type="STRING" id="190192.MK0855"/>
    <dbReference type="EnsemblBacteria" id="AAM02068">
        <property type="protein sequence ID" value="AAM02068"/>
        <property type="gene ID" value="MK0855"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0855"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.898"/>
    <dbReference type="HOGENOM" id="CLU_044308_3_0_2"/>
    <dbReference type="OMA" id="ARHGLFD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="PTHR23133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158190" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <sequence length="201" mass="22292" checksum="6D5889526093EF5F" modified="2002-06-24" version="1">MNSVRTADVKRTTKETEVEVSVNLDGSGRAKVDTGLPFFDHLLHQFAFHGRIDVEIKARGDLEVDDHHTVEDVGICLGKALNEALGDREGIRRIAWALVPMDEALVECAVDISGRPYFVLKGYRPRRNRIGSPPLSTENVSHFWKSFCDHAGVTMHVVVRWWDNDHHAIEAMFKAVGRALGAAKEIVGDGVPSTKGTLRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ6</accession>
    <name>Q8TGZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0370"/>
    <dbReference type="EnsemblBacteria" id="AAM01585">
        <property type="protein sequence ID" value="AAM01585"/>
        <property type="gene ID" value="MK0370"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0370"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.395"/>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="OMA" id="EPEENEF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="38"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="132"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="323" mass="36337" checksum="32AC13F1B5646A98" modified="2002-06-01" version="1">MFLPEIDDRCHELAERYGVRPYMVARYLTFLGRSETERLLKHMEDIRPAIRTNTILIDPEELRRVLEEKRGFELKRCPEPVDVGFWILNDPPISIGATLEYMMGYYVPQDAASMLPPVVLDPKPGDRVLDACAAPGGKTTHLAQLMDNEGTLIAIDVDPDRMRALKSNLARCGVANAVALRMNALDLPSTDWEFDRILLDAPCTGEGTIHKDPSRKTSRDPEDIATCARLQRRLIDAVVDVLRPGGVLVYSTCTFSPEENELIVQYAVDEHGLEPEPVDVGWADRGLRVPGVEPKVRRTCARLYPHRHGMGIFFVARLRKPLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT7</accession>
    <name>CARB2_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Carbamoyl-phosphate synthase large chain, C-terminal section</fullName>
            <ecNumber>6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">carB2</name>
        <name type="synonym">carB_3</name>
        <name type="ordered locus">MK1666</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="domain">
        <text>Corresponds to the C-terminal section.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">CarB is split into two genes in M.kandleri (MK0911 and MK1666).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUT7"/>
    <dbReference type="SMR" id="Q8TUT7"/>
    <dbReference type="STRING" id="190192.MK1666"/>
    <dbReference type="EnsemblBacteria" id="AAM02879">
        <property type="protein sequence ID" value="AAM02879"/>
        <property type="gene ID" value="MK1666"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1666"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1830"/>
    <dbReference type="HOGENOM" id="CLU_000513_3_4_2"/>
    <dbReference type="OMA" id="CVQAVFA"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145078" description="Carbamoyl-phosphate synthase large chain, C-terminal section">
        <location>
            <begin position="1"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="122"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="3">
        <location>
            <begin position="388"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain">
        <location>
            <begin position="390"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="148"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="542" mass="59225" checksum="671FE663AA9652E1" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU2</accession>
    <name>Q8TUU2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02874">
        <property type="protein sequence ID" value="AAM02874"/>
        <property type="gene ID" value="MK1661"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1661"/>
    <dbReference type="HOGENOM" id="CLU_1860713_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="137" mass="14666" checksum="46AB1265CD24B03B" modified="2002-06-01" version="1">MVVVVYHRPFSHFRVNHVALIAGNLGAKLLVYSEPKSRAALDGITQVLEHPEERGRVPVMVVDDLDDALEVVDGRVALLDPSADEGIKKLKPSDVLLTVPVELLEEIDIEPDLTVGVSAPVGELAALAVVLYELGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU4</accession>
    <name>Q8TUU4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02872">
        <property type="protein sequence ID" value="AAM02872"/>
        <property type="gene ID" value="MK1659"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1659"/>
    <dbReference type="HOGENOM" id="CLU_1943867_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="129" mass="14965" checksum="0A15E947758FC8D9" modified="2002-06-01" version="1">MPSISLQARERSPTNVHEARKVVEKVLKERDPELTYDQREAVRYLKKFGSLEPEDLEEAKEELRSILGDLTTNERTVEILVNKILEVQPRSEEEIKVLLESAGKRLLRRADEDVVRAILEVSERIAEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ1</accession>
    <name>Q8TUZ1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 11">Shikimate kinase</fullName>
            <shortName evidence="11">SK</shortName>
            <ecNumber evidence="5 11">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">aroK</name>
        <name evidence="14" type="ordered locus">MK1610</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="5 11"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1610"/>
    <dbReference type="EnsemblBacteria" id="AAM02823">
        <property type="protein sequence ID" value="AAM02823"/>
        <property type="gene ID" value="MK1610"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1610"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1773"/>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="OMA" id="WDVLVWT"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="PTHR20861:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="12">
        <location>
            <begin position="71"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="13">
        <location>
            <begin position="209"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM02823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="286" mass="30315" checksum="16DD2AD0A4697A4E" modified="2002-06-01" version="1">MGEGIAYAAGTVVNAISAKKGCAYALDLTVSVRAELSDSTEIHTDVEDTSLVERCVEVVGEHVGQELNFEIEVDSEIPIAMGLASSSAVSNAVVEALLKELGREPEPFEVVRLGVEASIRAGVTVTGAYDDACASYLGGLVLTLNDQHRVLDIRELPYPYAVILLPGGKVETSEVDVNRLELLAPAAETAFRRAMTGDYRGAMLINSAVYCSALGHEFEPVVEALEAGAAAAGLSGTGPAFVALCETKSDVREVSEVWSDRGEVLETRTVGPERARGAQARNRPHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV02</accession>
    <name>Q8TV02_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02812">
        <property type="protein sequence ID" value="AAM02812"/>
        <property type="gene ID" value="MK1599"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1599"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1761"/>
    <dbReference type="HOGENOM" id="CLU_1207616_0_0_2"/>
    <dbReference type="OMA" id="HNAGNER"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="27075" checksum="FC856EE0063C779C" modified="2002-06-01" version="1">MKVLALLVLAWLAYAFLGFVKFYATTGCLPLGRLAFVKLHDPDIRPGGLHEMVATNVAHTLGYSAVTIVHNAGNERMYGSWTEKNGVLVWNIAALDPRGNRASVDWHGALRELVFADRLRTATWVHDGPIGGPRFPKNSVIFWHGTVRNGFPLLYGGCGCEPYYYILANYGNVPFAITATVLGWFTPFLISPLEAFWELSHYKRLQYEYLKKVNGIRLINLKNYEGTSATPTDSISGNSVPRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV10</accession>
    <name>NDK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">MK1591</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV10"/>
    <dbReference type="SMR" id="Q8TV10"/>
    <dbReference type="STRING" id="190192.MK1591"/>
    <dbReference type="EnsemblBacteria" id="AAM02804">
        <property type="protein sequence ID" value="AAM02804"/>
        <property type="gene ID" value="MK1591"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1591"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1752"/>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137092" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="154" mass="17449" checksum="EDAA4E852045340E" modified="2002-06-01" version="1">MAEVQREFVMIKPDGVVRGLVGEVIRRLERKGLKIVALEMRQLDRETAEKLYEEHRDKPFFEDLIEYVTSGPVVVMIVEGRKAVKVVRNIIGATDPAEAAPGTVRGDFALDIGRNVVHASDSPESAEREIEIVFGKDLSTIDYERCDEEWLYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV37</accession>
    <name>Q8TV37_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02776">
        <property type="protein sequence ID" value="AAM02776"/>
        <property type="gene ID" value="MK1563"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1563"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1725"/>
    <dbReference type="HOGENOM" id="CLU_825414_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="SSF55797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="37051" checksum="D717887F8089A329" modified="2002-06-01" version="1">MILFSSSLSQYEGIELPTWRYVPPVLLAILLSSASPVAAGVAEMFHSEINEFRESLGLSPLKWSPSLARLAEQHAARMDETNELYHERCQGYNFENVARVPNTGEPTLTARLLAITVLSSPPHRWNVLEGNCEGVGVSISGDYAVLSQKISRASDDAVDVRVEVPPFHGVVSTAHRDDVEKAVLDAINREREQSGLPPLVLDKELSKRVLEELREAARTLSVTCSSGTLVILTRLQNPVEIAEDVLGVLRAVPAGRNFWVPKGRLGVAALEVTEVTMPDGNRLKLGVVWVGINVVEEMAGKNRPRRVPFPSYVAIAIPLCYLITRRQTFFTGRPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV46</accession>
    <name>Q8TV46_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrA</name>
        <name evidence="11" type="ordered locus">MK1554</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1554"/>
    <dbReference type="EnsemblBacteria" id="AAM02767">
        <property type="protein sequence ID" value="AAM02767"/>
        <property type="gene ID" value="MK1554"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1554"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1714"/>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_3" evidence="10">
        <location>
            <begin position="10"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine_dh" evidence="9">
        <location>
            <begin position="155"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM02767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="337" mass="35880" checksum="72FC4CD74E87A846" modified="2002-06-01" version="1">MRQARLFVIGLGAVGLGLMRLLAKKRDAYAREFGIDVRVVGVADSRGVWVKNDLDPAEVLKVKQELGTVAEVGESGDALEIMEDVEFDVLVELTPTDIETGEPGLSHIMKAIELGRHVVTANKGPLAVAYGEIMEAAEEAGVVVRYEATAGGAMPVFNLVRETLKSVDIHSIEGVLNGTVNYILTRMEEEGISLKDAIAEAQSRGIAEADPSMDIEGWDTACKVVILANAILGLDCTIKDVDVTGIEDITPEAIRIAEERGYRIKLIGRADSDGELSVRPCLVPKSDPVAKVRGVMNIVRLETDVAGDIYVSGRGAGPLETASAVMSDVLSIAESVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV49</accession>
    <name>SRP19_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="1">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp19</name>
        <name type="ordered locus">MK1551</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV49"/>
    <dbReference type="SMR" id="Q8TV49"/>
    <dbReference type="STRING" id="190192.MK1551"/>
    <dbReference type="EnsemblBacteria" id="AAM02764">
        <property type="protein sequence ID" value="AAM02764"/>
        <property type="gene ID" value="MK1551"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1551"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1711"/>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="OMA" id="VIWPAYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="PTHR17453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SSF69695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000135218" description="Signal recognition particle 19 kDa protein">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <sequence length="103" mass="12277" checksum="992515F3E8AF966E" modified="2002-06-01" version="1">MAETPTVKLKGKDRIVVWPAYFDADRSRSEGRKVPKRLAVRNPRLTELRHIAEKLGLNPKVQRDKRYPKRWWDDKGRLIVDKVESKRKTLLMIAEKLKERRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV50</accession>
    <name>Q8TV50_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III synthase</fullName>
            <ecNumber evidence="1">4.2.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hemD</name>
        <name evidence="3" type="ordered locus">MK1550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>hydroxymethylbilane = H2O + uroporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1550"/>
    <dbReference type="EnsemblBacteria" id="AAM02763">
        <property type="protein sequence ID" value="AAM02763"/>
        <property type="gene ID" value="MK1550"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1550"/>
    <dbReference type="HOGENOM" id="CLU_1212610_0_0_2"/>
    <dbReference type="OMA" id="IVPEEYH"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="PTHR38042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEM4" evidence="2">
        <location>
            <begin position="46"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="228" mass="25571" checksum="AD5DC46DC7D92957" modified="2002-06-01" version="1">MRALVVRAPGLERETVQAFKKVDLDAEVVCPVELVSLDRPKISAGLLSEYDAVAFTSPRTVEFLSEEEVKELRRSDVDIAAVGPRTREALERAGLRVDVMPTEYTTGKLAEELRQYGAVLALRSRRRTEDLRRTLESYGVKAEELEMYDLKPKRVEVNPRKFDVVCFLSAFTARCFLENVDPTEIPEPVVSIGPVTTKELRRAGLKVVEAEEHTVEAVAKTALRVIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV71</accession>
    <accession>Q49613</accession>
    <name>Q8TV71_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B, selenocysteine containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwdB_2</name>
        <name evidence="2" type="ordered locus">MK1527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1527"/>
    <dbReference type="KEGG" id="mka:MK1527"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1688"/>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="TAEVCHG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0711">Selenium</keyword>
    <keyword evidence="2" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="52"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="433" mass="48703" checksum="8AA92EB8C00976D3" modified="2008-02-26" version="3">MARKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETAKMLVEAKLPVLYGWSATLVEAQEKGVELAELVGGIIDNTASVUHGPSVLGLQDVGVPSCTLGEVKNRADTVIYWGSNPMHAHPRHMSRYTAFTRGFFRPKGREDRTIIVVDPRKTATARLADVYIRVRPNEDYELISALRAAVHGIEIEREEVAGVPTEQIYEVADLIKEASFGTLFWAMGLTMSRGRHRNIDNAICLIKDLNEYTKWTLIMMRGHYNVTGFNEVLAWTTGYPYAVDFSRGYPRYNPGETSTVDLLTRGEVDAMMVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV91</accession>
    <name>Q8TV91_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator containing a DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1501"/>
    <dbReference type="EnsemblBacteria" id="AAM02714">
        <property type="protein sequence ID" value="AAM02714"/>
        <property type="gene ID" value="MK1501"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1501"/>
    <dbReference type="HOGENOM" id="CLU_090237_0_0_2"/>
    <dbReference type="OMA" id="PHIKQKE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_45" evidence="1">
        <location>
            <begin position="46"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="268" mass="29499" checksum="EAFF45B988CDC981" modified="2002-06-01" version="1">MEEGPIRVPNELKDRRELMKSLILAFIGLRQPRVHLGDIGGELGVSLQAIHNYVKELIDEGLVEKKGRAEYVLTDKGAEKALESINNIRRFSAKIAEALGKRMTWAAIAAEDIRKGEEIYLYMEDGLLYASKSRKTGARAIAQADAEKGQDLPVTNIEGDIPGLERGEVVFVCVPSAREGGTRKLDLDRLKEVLEKEDYDLIGAAGTAARAALNMLGIEPDLKFGVVDGAIMAALKGLKALVVITTPMLRRARDKAERRGAEYRIQYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA2</accession>
    <name>Q8TVA2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nfnB</name>
        <name evidence="3" type="ordered locus">MK1490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1490"/>
    <dbReference type="EnsemblBacteria" id="AAM02703">
        <property type="protein sequence ID" value="AAM02703"/>
        <property type="gene ID" value="MK1490"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1490"/>
    <dbReference type="HOGENOM" id="CLU_059362_3_1_2"/>
    <dbReference type="OMA" id="CAIAAYD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Nitroreductase" evidence="2">
        <location>
            <begin position="56"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="233" mass="25804" checksum="C456369AC1BEBE1B" modified="2002-06-01" version="1">MSPRLVVPAAVVVVAIGLLAYWYLYPSEGASEVPIKAKIELPKPLELKMSVSEALKKRRSIREYRDESITLRELATVLWAAQGITDPRGFRTAPSAGALYPLRVFVVVRKVEGLAPGIYVYDPKTHTLGLVRRGNFTTELQRACLDQEWVGHAAVDLVIVGYERVLQPRYGERSFRYMALEAGHVGQNIYLACTALGLGTVAVGAFYDDRVKEILGITEGDAVPLYVFPIGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB6</accession>
    <name>RL13_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13</name>
        <name type="ordered locus">MK1476</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB6"/>
    <dbReference type="SMR" id="Q8TVB6"/>
    <dbReference type="STRING" id="190192.MK1476"/>
    <dbReference type="EnsemblBacteria" id="AAM02689">
        <property type="protein sequence ID" value="AAM02689"/>
        <property type="gene ID" value="MK1476"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1476"/>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="OMA" id="GMLPWKT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_L13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="PTHR11545:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000261839" description="50S ribosomal protein L13">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <sequence length="159" mass="18324" checksum="1C264A7A26ED053A" modified="2002-06-01" version="1">MVEYAHSKPVDPEEWTVIDAENAVLGRLASVVAKRILKGERIAVINTEKAIITGKKNTIKEEWLQKIQRGDPKKGPFYPRRPDLIFRRVVRGMLPWKTKRGREAFKRLRAYIGTPRWVEEANIEPERVAEADMSRLGHLWYVTLGELSEELGYQMPGGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB8</accession>
    <name>RPO3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo3</name>
        <name evidence="1" type="synonym">rpoD</name>
        <name type="ordered locus">MK1474</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB8"/>
    <dbReference type="SMR" id="Q8TVB8"/>
    <dbReference type="STRING" id="190192.MK1474"/>
    <dbReference type="EnsemblBacteria" id="AAM02687">
        <property type="protein sequence ID" value="AAM02687"/>
        <property type="gene ID" value="MK1474"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1474"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1630"/>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="OMA" id="ECLRHPE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132755" description="DNA-directed RNA polymerase subunit Rpo3">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <sequence length="275" mass="31526" checksum="4EE0C1847BD6C621" modified="2002-06-01" version="1">MSPLRVRLYDYRKADVERATFIIEETSAEFVNTIRRALYTLVPTLRIEEVIIYENDTPMYDEMLAHRLGLIPLRVDDIDQFELPDLCDCGGKGCEKCQVRAELEVEGPTKVYARDLKFDHPDVEPAFPDTLITEVGEDQRIRLEVIAVPGLGLEHAKWKPVSAVGYKGLPELEIDEDKLKEKKITYECPQGIIRIENGEVVHIDEDRLPECRMYKEYERETDGAVRVRLRDDAFVFNVETDGSMSLDTAILKALDAIEHKLESLKKNLQKEVSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF8</accession>
    <name>Q8TVF8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted calcineurin superfamily phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02644">
        <property type="protein sequence ID" value="AAM02644"/>
        <property type="gene ID" value="MK1431"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1431"/>
    <dbReference type="HOGENOM" id="CLU_063034_1_0_2"/>
    <dbReference type="OMA" id="CGHCHIP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="4"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="30481" checksum="81E27DACF4B49B62" modified="2002-06-01" version="1">MNAKIVHISDLHISPYRKSWSPEVFYRGIEQINDLRPDVVIVTGDLTDNGLVREYEEVSSLLEKIEAPVVPVPGNHDARNLGWMTFEDVFGDRYRVERVSADLYVVGLDSSEPDVDYGQLGRERQEWLEETLRRIPGGACKCIAMHHHLLPVPGAGRERNVLVDAGEMINLCIKYGVDLVLCGHRHVPFAAKVEDTVVVNAGTFSATKLRGYSRNSFNVIEFSESTVSVNLYEITTERKLELARYKPVVREGEYRLVRVKGIADILRESA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG3</accession>
    <name>HIS8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisC</name>
        <name type="ordered locus">MK1426</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVG3"/>
    <dbReference type="SMR" id="Q8TVG3"/>
    <dbReference type="STRING" id="190192.MK1426"/>
    <dbReference type="EnsemblBacteria" id="AAM02639">
        <property type="protein sequence ID" value="AAM02639"/>
        <property type="gene ID" value="MK1426"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1426"/>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_2"/>
    <dbReference type="OMA" id="IWLNANE"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153497" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <sequence length="391" mass="43959" checksum="52D182A5CC9AFEB1" modified="2002-06-01" version="1">MDRVRPLAGGRNVRIREAVLNIDPYVPGKSKEEIAREYGIEPDEIVKLGSNENPLGPSPKAVKAAKRELERLHEYPEPLAPPSLYEAIIDYLADPPYPAGEPVEITREHLVVGGDGADEIIDVLTRVLVDPGDPVVIPVPTFSQYGISARACGAEVRKPRFDPERGFELDEDSLFEALDREVRLVYLCTPNNPTGNRIRERVVRDVVEECRGVVLIDHAYVEFADHDYTPLALEYDNVLVLRTCSKALGLAGARVGYGIANPELIEHLHRIKPVFSLTRPSAAAAEATFRDRDYIEKSVRLMIESRKYLYRELRKLDRLTPFPSEANYLLVDVSNTGMNASEFTEELLKRGVIVRDCSSFEGIEPFYVRVSTGTLEEDRKFIEVVKDVLEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG7</accession>
    <name>DAPB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">MK1422</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVG7"/>
    <dbReference type="SMR" id="Q8TVG7"/>
    <dbReference type="EnsemblBacteria" id="AAM02635">
        <property type="protein sequence ID" value="AAM02635"/>
        <property type="gene ID" value="MK1422"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1422"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1578"/>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_2"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141519" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="275" mass="29189" checksum="CB68BA12EB1466E6" modified="2002-06-01" version="1">MIGVVVLGATGRMGRRICRMVIEDEELELVGAIASPTSKHLGRDVGLVIGVGETGVEIAPPTALPNIAKDADVAIDFTVREATLENAPKAARAGLDLVIGTTGFSDEDLRVLEHEIEEAGVSAVISPNMSLGVNLLFELTRQLARVLGDNGFDFEIVEIHHRHKVDAPSGTALELAAIIEEELGKGEKVFGREGNVGPRDDDEIGVLAVRGGEVVGDHTVMALGEYERIELTHRALSRDAFAKGALVAAKFVVEAPPGIYSMRDVLFGGKRGEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH2</accession>
    <name>Q8TVH2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02630">
        <property type="protein sequence ID" value="AAM02630"/>
        <property type="gene ID" value="MK1417"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1417"/>
    <dbReference type="HOGENOM" id="CLU_1029013_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009343">
        <property type="entry name" value="DUF1002"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06207">
        <property type="entry name" value="DUF1002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="29937" checksum="D23456828BAE4797" modified="2002-06-01" version="1">MRFVRKEITVVVVIAVIVLPAAGYYEVNYGRGSVERPVVTYGETTWRTELPTAVELFKEVGVDPTRFHRTIVTAEETNRVAAEVTGRRYTPSQIYSCATVTPYDEPPRRYQEVEGYRIYVGPEITVCKPETYAEALASIGAPPCCVTIRSPVRATGEAALAGVYKAMREAGVEITDRDARFSQAVLEAVKGAGDDPRRRAAAVTVVVVCVFRGADDPQKARDVQDEVENVYGVNLPPETAVHAAQAAKLAEEGAEYSWWWLFKRLLAYWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI6</accession>
    <name>Q8TVI6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted P-loop ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1403</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02616">
        <property type="protein sequence ID" value="AAM02616"/>
        <property type="gene ID" value="MK1403"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1403"/>
    <dbReference type="HOGENOM" id="CLU_649917_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="13"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="311"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="422" mass="49486" checksum="F646B95337C16B9D" modified="2002-06-01" version="1">MSCGEDKEVLFEALDAVEEKYDLRKSQYEMLEEIRDQLAECDSVAVLTTRPGSGKTWLLRRIAREYRERVFYSVPSPDLAEREYKKFKEWELSVDLWRRVEDYDEPCQVKLPELLGELEQEELFEVMAHAGSAQDEPKCYPWFYRKEGPLLKPLEDEELREKVRRALYRENPFIKGFAPWDSCGNCEIIRQLREPARIVCLSFKKLLSAPFLYASHRCAREALGKEPFVSEEDWEEALEGSLVVLDDAHVLPGALIVEIRGYVRVGSRTLMDELKDLALDQSHMWSVVDWNRDVRKVKKALEEKTKFREVYDRLTYELEDWIEELRELVERECRADYLDAKKNLSLLNELIERSEEAGIREWVVVKSPRRGRPILTFAPVVKNLEQLLKLLMGPEYFELPVAYLVAENRPTLSELDRRGVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ4</accession>
    <name>Q8TVJ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02608">
        <property type="protein sequence ID" value="AAM02608"/>
        <property type="gene ID" value="MK1395"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1395"/>
    <dbReference type="HOGENOM" id="CLU_1954681_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="128" mass="14579" checksum="AA4A49CBD690F4F0" modified="2002-06-01" version="1">MSLRCPRCGGPVRPSLDRLECEECEWSKELRSRPRKDTRLKWMKEYARRFLREEFDDCGVSEVIVRGPRGKGAGYLAATVYVDDHHKAIGKDGSRVKEVEEKLETLADELGVPPVRITVHPSSALGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL8</accession>
    <name>PDXS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="1">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxS</name>
        <name type="synonym">snz1</name>
        <name type="ordered locus">MK1371</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVL8"/>
    <dbReference type="SMR" id="Q8TVL8"/>
    <dbReference type="STRING" id="190192.MK1371"/>
    <dbReference type="EnsemblBacteria" id="AAM02584">
        <property type="protein sequence ID" value="AAM02584"/>
        <property type="gene ID" value="MK1371"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1371"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1525"/>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="OMA" id="RYANRGW"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PTHR31829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00343">
        <property type="entry name" value="TIGR00343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000109436" description="Pyridoxal 5'-phosphate synthase subunit PdxS">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <sequence length="295" mass="31918" checksum="6AE4DE7C367004E0" modified="2002-06-01" version="1">MKTGTWRVKTGFARMLKGGVVMDVTNVEQAQIAEDAGAVAVMVLEKVPADIRAAGGVARMCDPAKIEEIMDHVTIPVMAKCRIGHVAEAQVLEAIGVDMIDESEVLTPADEEHHINKWEFEVPFVCGARNLGEALRRIAEGAAMIRTKGEAGTGNVAEAVRHMRIIRREISELTRLDKEELYGKAKEYGVPFDLVAEVASLGRLPVVNFAAGGIATPADAALMMQLGADGIFVGSGIFKSDRPQEMAEAIVEATAYYDDPEVVAEVSKNLGDEVAMRGLEISEIPEEERMQLRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN1</accession>
    <name>Q8TVN1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-10 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02570">
        <property type="protein sequence ID" value="AAM02570"/>
        <property type="gene ID" value="MK1357"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1357"/>
    <dbReference type="HOGENOM" id="CLU_1159064_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="239" mass="25702" checksum="B392B387F0AAD22D" modified="2002-06-01" version="1">MIAGGPGSGRVVRAGGPGRGPRAGRRAPRGPPGQGRGGGGRGGGGGRGGAGAKLSRAGKVLRSPGKFLRALKKFKVRKRYSKRERKRLLLLYWKVRLYYRTVAEGVLLYASFALAIVIFALDFLTDDLELLRETWFERSLYLTSGGAFLAIFGEMVVANRAVSAFSEAVLRGGGLPAALGAFRAWAVYGMGMLLAMYVALVPLIIALTRKEPDVRTLPMLAVPLATGIWALWWGLWVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN4</accession>
    <name>Q8TVN4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-10 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02567">
        <property type="protein sequence ID" value="AAM02567"/>
        <property type="gene ID" value="MK1354"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1354"/>
    <dbReference type="HOGENOM" id="CLU_910936_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="33974" checksum="7575E408D09B2F1B" modified="2002-06-01" version="1">MLEILALTVLTVPEDPGRFLEHVLENPKVLLAAGGGRGGAAGGRGGGFGGRGGGRGGGAGAKLSPRAGEILESRGKFLRELRRPRSNLKCCPSRHSERERRRLLRLYGKVRVYYSTVPPTLTIGGIVALLSTPFTVFLPLRRALNRVWERRYGPRFRGGDPLWYLRYLDEVRRFNERIAGRLWAISALSLLTVVAEGELSYQAAYAFADAVVEGGEPLSAFYDWAVLGMEALLAVYVATSVVIALHLVRTRDPKHRRYCPIFRACLPRPYRRRAWRTLRTLTVPVSILPVITGAWALRWGLWVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP2</accession>
    <name>Q8TVP2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02559">
        <property type="protein sequence ID" value="AAM02559"/>
        <property type="gene ID" value="MK1346"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1346"/>
    <dbReference type="HOGENOM" id="CLU_870479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="35024" checksum="8BA65F8CAF582B54" modified="2002-06-01" version="1">MLPYLVLGLVLALAPASGELLIRGSLSEDKAFLKEYLESEVLPKWKDDPRVIVDYSDWHHRLVYLPDRDLFVGTDGPATVTVGPGSVRVEVQLATVTEAGRESIQVSKSVRDPAVVANSGFDSVTVLVGDYVCGVEEKLVVGGPPYYIAVLKEGDRVIDREDLGGLSVSDVEYEVYSDRVVMRVLGFGEGGKPLAIIVTFDLKDGRILVDREEITDAALIEKLSGELVEYPEGAVLRTGSGVEPLYVKFGAPRDDPLVRFIDKVVRMYGQGASEEELRRVMSEHSNVEFLFPERGGGEESGLPVWVLPLLPVPLTVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP7</accession>
    <name>Q8TVP7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1341</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02554">
        <property type="protein sequence ID" value="AAM02554"/>
        <property type="gene ID" value="MK1341"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1341"/>
    <dbReference type="HOGENOM" id="CLU_2645920_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="76" mass="8062" checksum="E4C3A06D04CDD2AD" modified="2002-06-01" version="1">MTVSLTRALGMPSDVLHRPVFVPRLPPEYALVGLAAYLTALATRHYLGPLDYLAFSALVPAEGFFVGCRSPSGGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS5</accession>
    <name>Q8TVS5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02526">
        <property type="protein sequence ID" value="AAM02526"/>
        <property type="gene ID" value="MK1313"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1313"/>
    <dbReference type="HOGENOM" id="CLU_619135_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="488" mass="55967" checksum="08BC397F2D9488E7" modified="2002-06-01" version="1">MRRAFPSTVLPVALILLPLPGHGAVWVDPEVLPDVSVKSWLIVEDVHWKEETTWHGDKVLDYVDAEYIAAISTRNPGKPVTLHVLLPPDAEVLEARFYPEIEWGEAEEVPPAEEWMYIIWKEWERVEPADGSEVTWEFLDETWKWTQLDFRVTPEAHDSALVLELRIPAVHRVPDLQMVEPVWNGEGISFPYGVKLKYLYDIGAVEWPVPVQPYPAAWCLDCALRTLSVKVELFVWPDGVFTHVPEGAPWTSYEVHVMDHLMLALGDVVTIDVVGIDPRKMSYEEYERKRAEVVRSEWPRVVERFGFPYVPVVMVVEIRQKRVIFDISLTPDPVEAGSEVTVLVRPRLYWWGPWCADEWPWDCWPEEGVLRFELKDERGSVTDLGAVELLPGGYVDGGLVMFRFRAPEEPGDYTLIVRYEGPYGEYRERLSFTVKERGRRDGQGRTPPSNPPESPPEQPPEKPPEKGGKRGFPVLVPPVIPPRCRARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV6</accession>
    <name>Q8TVV6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02495">
        <property type="protein sequence ID" value="AAM02495"/>
        <property type="gene ID" value="MK1282"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1282"/>
    <dbReference type="HOGENOM" id="CLU_074023_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35847" checksum="5D807FA732B4A062" modified="2002-06-01" version="1">MPDPTLTLLLVALQHPTHGDCSAVLVRTHGGYAVAYRRDATYRATLHLEVTTWAGRGALREYKLRNGRFCHTVITEDGWVITIGGADDESVNRGLEELGERVVLAGEVTEWDLERALDLLRRIGIGHFLVGSPDGTVGVAVYRYGRTLLEVLRLAEGEYVLVPNDPRYYDRGRFEDYDTDPVRAAAEIAGLDPYGVNRRDVLVYLVEPRDGRATVRVWVAYDGGALLGRRGAGPDPVEFMGCYVPAEWIPRLPDLFELGTVTLGRPMPTRAPEGNSRSAGSPVWWPVPVPAGRPGGSRWRRATDDLHARDPTTEGVRRVGTAVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW05</accession>
    <name>Q8TW05_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase subunit of an iron-regulated ABC-type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sufC</name>
        <name evidence="5" type="ordered locus">MK1233</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TW05"/>
    <dbReference type="STRING" id="190192.MK1233"/>
    <dbReference type="EnsemblBacteria" id="AAM02446">
        <property type="protein sequence ID" value="AAM02446"/>
        <property type="gene ID" value="MK1233"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1233"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1336"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="OMA" id="ILITHHE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="237" mass="26015" checksum="C8D0BA2B52024451" modified="2002-06-01" version="1">MALLEVKDLRVESEDGTEIVRGASLTVDEGEIVDLIGPNGSGKSTLAKTIVGCSGYEVVEGEVRFKGRDITDLPMYERARMGLTMSWQEPARFEGLTVGEYLSVCTDDEDWARRCLRIVGLDPEEYWDRECGENLSGGERKRVELAAVMAMRPDLVILDEPDAGLDMSSMEDLAKVIETMREEGSAVLLITHNRDLAEQVGDRAYLMMDGKIVDEGDPKDVVLKCLMCGGGLVCGAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW24</accession>
    <name>Y1213_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein MK1213</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1213</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW24"/>
    <dbReference type="SMR" id="Q8TW24"/>
    <dbReference type="STRING" id="190192.MK1213"/>
    <dbReference type="EnsemblBacteria" id="AAM02426">
        <property type="protein sequence ID" value="AAM02426"/>
        <property type="gene ID" value="MK1213"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1213"/>
    <dbReference type="HOGENOM" id="CLU_155669_0_1_2"/>
    <dbReference type="OMA" id="MIMLVDI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000219911" description="UPF0237 protein MK1213">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <sequence length="90" mass="9798" checksum="3399554AE42670F8" modified="2002-06-01" version="1">MTRAVVTVIGADRPGIVAGISSVLAEHNANIEDISQTVLRDLFAMVMLVDLSEADVSVGKLREELQKAGEELGVDVIVQHEDVYRAMHRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW35</accession>
    <name>Q8TW35_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ni,Fe-hydrogenase I large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hyaB</name>
        <name evidence="2" type="ordered locus">MK1202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1202"/>
    <dbReference type="EnsemblBacteria" id="AAM02415">
        <property type="protein sequence ID" value="AAM02415"/>
        <property type="gene ID" value="MK1202"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1202"/>
    <dbReference type="HOGENOM" id="CLU_1335058_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="22385" checksum="DF0BDBC879D70A4F" modified="2002-06-01" version="1">MDSWSEKLDVDVWELLKPCARNRNIARILEALLLTRLALHWIDDLPTTGTTYKDPSRRSGCSGTGVIEAPRGTLVHRVSVGSDGKVFNYEIITSTNLNHAPIEESMVGERVYVDEKLAAAKRQLSESERFLLGDACRAARSFNPCNSCAPHAIVKVVKKGWTNSGAPTEVPRGGTEEESAEVISGRGAHVERGVPLRRDAAPGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW58</accession>
    <name>Q8TW58_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1178"/>
    <dbReference type="EnsemblBacteria" id="AAM02391">
        <property type="protein sequence ID" value="AAM02391"/>
        <property type="gene ID" value="MK1178"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1178"/>
    <dbReference type="HOGENOM" id="CLU_306038_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13517">
        <property type="entry name" value="FG-GAP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="SSF69318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="968" mass="107054" checksum="7F0890392554E7C5" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW61</accession>
    <name>Q8TW61_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02388">
        <property type="protein sequence ID" value="AAM02388"/>
        <property type="gene ID" value="MK1175"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1175"/>
    <dbReference type="HOGENOM" id="CLU_2021521_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="122" mass="13739" checksum="7D05A25EEE1A2752" modified="2002-06-01" version="1">MSVISKDPGTITVVVRVDDEALSKAYSADKGARITDWFGPVELSPGSHDVGIHLYLDGQEVRWYGSVVKVMSGREHVRDHDEYEHHREEHDHGGTTRINPRRSDFPPSGSRSRGPRDHPNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW64</accession>
    <name>Q8TW64_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02385">
        <property type="protein sequence ID" value="AAM02385"/>
        <property type="gene ID" value="MK1172"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1172"/>
    <dbReference type="HOGENOM" id="CLU_958504_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="290" mass="31106" checksum="B0F361699EF6A138" modified="2002-06-01" version="1">MVAHRGRLFVETDGKLLGSCEASLIVTEENVVVVPREPIEGVDTSDLVVESVASRFDSAELEEGNFTPPRSRVVMTPPTAASMIDLGLALKRFEVDGVTVLTVVGPAPAYPFERRILAPEGEMTLRFEPKDPVAAEGSLRAAARAAVEGTVGETVGRSKAVRLCKVLVELNQTVGHGTLRLVPRDENVLLVLQPEEDIAEEFKDAVGKSQIGIALTPPELSAVFSGKAVVKHGKEDTVHVLDARKTIEFRAEGKGWALRAVPTDVIAGSTARVLLRTAAELIVHEGLRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB6</accession>
    <name>Q8TWB6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02332">
        <property type="protein sequence ID" value="AAM02332"/>
        <property type="gene ID" value="MK1119"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1119"/>
    <dbReference type="HOGENOM" id="CLU_2613658_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="78" mass="8119" checksum="411418E7263A5FA8" modified="2002-06-01" version="1">MPPIPIVLALLIMIGPASGAIVEFSEEPELVGKLISRVHDLRATEGAVRELNAAFAPGEVASVSGVFVYPPGSPDRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC4</accession>
    <name>Q8TWC4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02324">
        <property type="protein sequence ID" value="AAM02324"/>
        <property type="gene ID" value="MK1111"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1111"/>
    <dbReference type="HOGENOM" id="CLU_625025_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="438" mass="48685" checksum="6A6726868F1DFE29" modified="2002-06-01" version="1">MLLFSTAIPAEVKRGLGEAGLRFGTVFEVVGGLVLPYIAEFSEEYHVVEGLARIDKEKVRSQGAVTPALGIPRKKVRNYPLELAQVTLKVRDVLTYKAAAAYFAWIAREFDVSVASAVRAILEDSEFLEALVEILCPVNPKAAEVLAGFRGRMWRDEARWLKFEEMARKALELAEELGDNRLRTAAELILRGKSSGPTVPGARGDHDGVYVYDIGKVRNFLVERMLKYGESSRKQPDEEIIEALVTEYGVDFEDVHYAIAVLSEGRIPAGPLRTDARVLLDNVAGFPGKVERDVPAPWMRKSYGAWFSDLRRLVPVTEENFNDVMRAKGYGISLEDVEEDSEWYLGKTVGKVLLGEDAKVPPEAREITQTENVKFVNAALRPRHALSREEVEEIREVLGSVETDVPVPIEGIVVEKALTEFGKLPMPEDISAAISRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE1</accession>
    <name>Q8TWE1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK_11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02307">
        <property type="protein sequence ID" value="AAM02307"/>
        <property type="gene ID" value="MK1094"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1094"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1149"/>
    <dbReference type="HOGENOM" id="CLU_1811448_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="142" mass="16022" checksum="B4AECAA8324A02FD" modified="2002-06-01" version="1">MTPIDPEEDARLEWERSMARLGLKYVPTVVISRVRGVALTLEIFAPVSAPAGSSAIVFVRPYLLTLRGGGPLWEGTLEFRLNGAGNSELGRIDLSEPEVWVGDWVEFEFYTPEESGKYRLVVRYEGPYGTVREEFQLRIVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE2</accession>
    <name>Q8TWE2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-methylhydantoinase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hyuA_2</name>
        <name evidence="2" type="ordered locus">MK1093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1093"/>
    <dbReference type="EnsemblBacteria" id="AAM02306">
        <property type="protein sequence ID" value="AAM02306"/>
        <property type="gene ID" value="MK1093"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1093"/>
    <dbReference type="HOGENOM" id="CLU_014140_2_0_2"/>
    <dbReference type="OMA" id="PAYKVWE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="192"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="482" mass="52090" checksum="9C3B1F9E47057E55" modified="2002-06-01" version="1">MRVLGLDTGSTHVDAVVLEDGEVEDRVKVPRDGKLLEPTRRALRALDADERRVSTTLPINALTRGRTDPVHLVLIPGPGLDPEPLLDLADRADVLPGYVDHRGDIVEEPDPDAVEPSGEDHALAVVAKHSHRNADPERKIIEAHRETYVEAVPGYHLPYGNFPRRVATAVLGARVKRVTRRFLEALGRVDGVLRGDGGLQTPEEALRVPVNVLHSGPAAGVLGAAYLTGRDDFLLADVGGATIDLTEARGGRPVTEEGAELFGYPTAVRAAIVRSLPYGGNAVLTGEGITNDTATPACYGGSEPTLTDALVVAGYHDPEPDGDPSKSRRVLRELGDPHEVAEETLETIRRELLRRFETHDSVLWAGALAPALRELTGVGEVVPHHDVCNAVGCAVARHAREAVVYVHTERGYGHVAPAGERFEVDRGRVYSREELVELAIEAAGFEPDEVRVRAFEIVRGGVRVGQWAEVYLYRTPGVEPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ0</accession>
    <name>Q8TWJ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02257">
        <property type="protein sequence ID" value="AAM02257"/>
        <property type="gene ID" value="MK1044"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1044"/>
    <dbReference type="HOGENOM" id="CLU_1648317_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="160" mass="17906" checksum="91504BA22B230CCA" modified="2002-06-01" version="1">MDWALKYQDASLVEFDGRKVLGWAYSSTRLESCGPYLGYTALIAATLKLAQELGLGSKAVEKMRSVGVTLEDEAEWVAHEWQEIDGLGYFLYPNSKAEHKTVTPSSQLYGLTCAVAFSHPEVLLKRTTADVWKLVPKKGAWKVPVWPVVTVVLLFACRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP8</accession>
    <name>Q8TWP8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator containing DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0984</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02197">
        <property type="protein sequence ID" value="AAM02197"/>
        <property type="gene ID" value="MK0984"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0984"/>
    <dbReference type="HOGENOM" id="CLU_1860712_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="137" mass="15468" checksum="72685DC161D132C5" modified="2002-06-01" version="1">MVSADDLTYREKLVLLALYEEFDGGPAGVKKLAPVVHMDKTKVSRALNALEEKGLVEFEHMEGRRLTDEGEKIAEEIAEEMELPEDEGPYQCSDCGRKYKHLRLKCAVCGGEIEVNKDHPDAAKAEWNLERARMGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ0</accession>
    <name>Q8TWQ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotidyltransferase of the DNA polymerase beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0982"/>
    <dbReference type="EnsemblBacteria" id="AAM02195">
        <property type="protein sequence ID" value="AAM02195"/>
        <property type="gene ID" value="MK0982"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0982"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1029"/>
    <dbReference type="HOGENOM" id="CLU_1217517_0_0_2"/>
    <dbReference type="OMA" id="MATPWHL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009185">
        <property type="entry name" value="Nucleotidl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005928">
        <property type="entry name" value="Nucleotidltrnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="229" mass="25771" checksum="7B1A1B5A983E8DE7" modified="2002-06-01" version="1">MDLREVRYTEEHWELLRTLRDRARKVVETLSQFGLEGWVHGSVARGDVRPGSDVDVFIPTPVSPHVVDAFMDAAPFEVVGVTAVLPTPRDCVRIKVAMEEDVEVTFPITPPTDRELEFFDFSGKLTPDSLERDERVPGVDKRLRMIEPKPWGHVEYSILGREGEVATKLGVSTQLIHERVRALTRRDKVGVQGVHAKVEGRPGEPVIELLRELGRRNPKAAEKLSELGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU2</accession>
    <name>Q8TWU2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0940"/>
    <dbReference type="EnsemblBacteria" id="AAM02153">
        <property type="protein sequence ID" value="AAM02153"/>
        <property type="gene ID" value="MK0940"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0940"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.984"/>
    <dbReference type="HOGENOM" id="CLU_022610_0_2_2"/>
    <dbReference type="OMA" id="IHYHETV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="3">
        <location>
            <begin position="26"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="276" mass="31517" checksum="4DCDEE90E76B70AC" modified="2002-06-01" version="1">MLSREEFARRLEEELPDVADSVPHRWQELGDVVVIRLFDERAWAYRREVGRILREVTGARSVVALRRVSGTFREPVGEVVAGDRNAETVHRELGIRFKLDPTRVMFARGNLEERRRLLESDLEGKLVFDMFAGIGYFTLPAALAGAEVIAAELNPVACRYLVENARLNGVEGRVRVFLGDCREVARFVRAADRVLMGYLKGTLKFLPYACRAVRDGGVLVVHEVFQKRWGEDRVAREVLNALPDGFEGEVLEVRRVKSFSPALDHYAVELRVRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX1</accession>
    <name>Q8TWX1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein, MK-44 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02123">
        <property type="protein sequence ID" value="AAM02123"/>
        <property type="gene ID" value="MK0910"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0910"/>
    <dbReference type="HOGENOM" id="CLU_430012_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="636" mass="70405" checksum="F7E784ECD78F6E71" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX3</accession>
    <name>Q8TWX3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA sulfurtransferase</fullName>
            <ecNumber evidence="8">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">thiI</name>
        <name evidence="12" type="synonym">thiI_2</name>
        <name evidence="12" type="ordered locus">MK0908</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0908"/>
    <dbReference type="EnsemblBacteria" id="AAM02121">
        <property type="protein sequence ID" value="AAM02121"/>
        <property type="gene ID" value="MK0908"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0908"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.950"/>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="OMA" id="SMPEFCG"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="11">
        <location>
            <begin position="49"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="10">
        <location>
            <begin position="168"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="171"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM02121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="374" mass="42018" checksum="2006CED203EEB77A" modified="2002-06-01" version="1">MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFGVERWTRTLRVDSHDPEDVFTEMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIRRYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGLPYGSQSRVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEGLEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFPVFRPLLGFDKNEVQRLSERLGLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHRGLRGSGVAGSEEYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX7</accession>
    <name>Q8TWX7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protease of the collagenase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02117">
        <property type="protein sequence ID" value="AAM02117"/>
        <property type="gene ID" value="MK0904"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0904"/>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="OMA" id="HFNFFSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="38557" checksum="06FF1F383B305E74" modified="2002-06-01" version="1">MKFWIPHPGHTSGLEALLSGIDDLGRVEAVYTGGSPDEVGTGRPNLHYPRLDEVREQVELAHSHDVLYDVVINSTCPLRGEPTRRVAERYSNYLEELEKAGVDGVVVADPFVLKLAAEVFDRITVSCLAFVNTPEKAEFYADVATAITVDTSFNRRFEVLEELSRLQVELKVIVNEGCLLDCPYRPFHFNIFSHLYGPDEVSVYDDYYYRRCIADRLEHPELIIKSPWIRPEDLGHYLEYVDAFKISGRSHQVEWIVEVVNAYLEGRWDGNLLHILDCPRELRDVFYVPNPELDGAIERWKECDFRCHECGFCRELADRVVEVREFKSTVVGEVSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY4</accession>
    <name>COFH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="1">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofH</name>
        <name type="ordered locus">MK0897</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWY4"/>
    <dbReference type="SMR" id="Q8TWY4"/>
    <dbReference type="STRING" id="190192.MK0897"/>
    <dbReference type="PRIDE" id="Q8TWY4"/>
    <dbReference type="EnsemblBacteria" id="AAM02110">
        <property type="protein sequence ID" value="AAM02110"/>
        <property type="gene ID" value="MK0897"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0897"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.939"/>
    <dbReference type="HOGENOM" id="CLU_040406_1_0_2"/>
    <dbReference type="OMA" id="ATMMFGS"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00293">
        <property type="entry name" value="((2_3_4_5-tetrahydroxypentyl)a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141719" description="5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="56"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="369" mass="41064" checksum="3BC771AA5C211AFB" modified="2002-06-01" version="1">MRDSPDEVSVDELVNMAVAGGIDEGTALDALQGKLDPYKVMRAAHEARLKIVGEHVTFVVNRNINFTNVCINRCRFCAFRRDPDDPDAYRMTPEEVGERAAEARDAGATEVCLQGGLHPEATFEYYLEMLDEIKSQAPDIHVHGYSPMEVKYCAKLAGEDIEDVLRELKRAGLDSMPGTAAEIFSPEVRKRLCPDKLEADEWEHIIRIAHELGIPTTCTMMYGHIDSPRDWIDHMKRLRGIQEDTGGFTEFVPLSFVHSNAPIYRRGGARPGVSGMTDVLVHAVARLYFGPLIPNIQASWVKLGVKLAQMTLHAGANDLGGTLMEENISREAGATEGEQLEPEEIVEIIREAGFTPVQRTTLYEPVKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ0</accession>
    <name>Q8TWZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02104">
        <property type="protein sequence ID" value="AAM02104"/>
        <property type="gene ID" value="MK0891"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0891"/>
    <dbReference type="HOGENOM" id="CLU_1478919_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="182" mass="19916" checksum="3C76D61D97EF97D7" modified="2002-06-01" version="1">MLKELWRSFAMALGAAWTLGLLALTVHILGLVWTIVLLLGISVLLGISVSRRVSPFIAVARGLGLLSLLMVNAARLEYLAPWLAMPVLGALYVFDRPAFYLVIAAAGAVLLLRALIEIHEAAVEADFRYRSKVQGLALAVREIWSGIMEALERLSTISAEKKEELSGTFTVAGKKLLRWMLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ8</accession>
    <name>Q8TWZ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease related to cation transporters</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0883"/>
    <dbReference type="EnsemblBacteria" id="AAM02096">
        <property type="protein sequence ID" value="AAM02096"/>
        <property type="gene ID" value="MK0883"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0883"/>
    <dbReference type="HOGENOM" id="CLU_111003_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="MgtE" evidence="7">
        <location>
            <begin position="50"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="17847" checksum="FF7A3981C2A5B2E3" modified="2002-06-01" version="1">MSSVWRGILRILRSMLVISIILAVWSSFTGSLLAKWEAKLEHNPGLAALLPVLMAAAGAAAASFGSRLSTYLHLGTFTIWAVLRDALVQGSALLLVIAGYAGLVTVAFSGRPSLILLAVESTGLTFLTSILVALGSAFTSVRLGMDPDDVVGPVVTTAADSVGIILTLTLAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX13</accession>
    <name>Q8TX13_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0867</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02080">
        <property type="protein sequence ID" value="AAM02080"/>
        <property type="gene ID" value="MK0867"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0867"/>
    <dbReference type="HOGENOM" id="CLU_2519772_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="84" mass="9728" checksum="02066E652E72C33F" modified="2002-06-01" version="1">MSERDTKKYDMIIPLGVPADVIAEVERRCDVKVVQREIERGGTKTTVLAFRGTKDALEEAKKVMEELIERRVEEWTKPRRRGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX18</accession>
    <name>Q8TX18_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02074">
        <property type="protein sequence ID" value="AAM02074"/>
        <property type="gene ID" value="MK0861"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0861"/>
    <dbReference type="HOGENOM" id="CLU_2379420_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="94" mass="11037" checksum="9432B426301C6C2F" modified="2002-06-01" version="1">MNRQDPIEKTKELFIKVSRILGEEQPEISVRREEDPSGHILNPWVANSEGADDVWPLVFEVRSKDGVAREFTLLVPVRLERRRLREILLKVFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX19</accession>
    <name>Q8TX19_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02073">
        <property type="protein sequence ID" value="AAM02073"/>
        <property type="gene ID" value="MK0860"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0860"/>
    <dbReference type="HOGENOM" id="CLU_1363653_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="200" mass="22932" checksum="4BA2C3FF3CA9A32B" modified="2002-06-01" version="1">MPCRTHALKLRPTCHPPHRTLRSRRRHPLEPVRPTPILPGGASVQVVRRIEIDRTRCEGASCALCVEVCERGVLKIKGEEVVIADLEACDACDECVRVCPNDAIDLYRYRERQFLSAMVRAMHGDGDGLEVARRVVMRGRDVFGDTWFYLRRELERTCRIKREAELEGKRAKLYECTCKVCGKKFKSNKKLDVCSECANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX40</accession>
    <name>Q8TX40_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02050">
        <property type="protein sequence ID" value="AAM02050"/>
        <property type="gene ID" value="MK0837"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0837"/>
    <dbReference type="HOGENOM" id="CLU_2127838_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="113" mass="13193" checksum="CDC189371613D6A5" modified="2002-06-01" version="1">MIDRLAKKYLTPEKVAELGFQITEELALRALNLIRNLRTEAKLEGDSYSFRVWDEKDRYEISASGEFRGSGFTLEFEKRVLDTRVRGHIEVEGEQVTELMRRLLEILKEGKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX98</accession>
    <name>Q8TX98_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="6">IPK</shortName>
            <ecNumber evidence="3 6">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK0777</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="3 6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0777"/>
    <dbReference type="EnsemblBacteria" id="AAM01991">
        <property type="protein sequence ID" value="AAM01991"/>
        <property type="gene ID" value="MK0777"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0777"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.818"/>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="OMA" id="HHNASEH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="PTHR43654:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AA_kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="239" mass="26327" checksum="A4F9A3C158F748AC" modified="2002-06-01" version="1">MFTVLKLGGSVITDKSKPKTAREDRIRRLMKVISDWRGDLVLIHGGGSFGHYAASRVSDLQKGVSEVRRAMHELLSIVEKVAVDSGVRVYPVTPATVLYSLNVLVDLLERGCVPLLYGDVVPDHDGDGFRIMSGDEIAERVSWLGPDRVGFGMSVDGVYPRTPEEGEPLRELSPDEARDLARQLEGSAGVDVTGGIAEKLRRAARIAERGAEVYMFDARDPENVDRFLRGEHVGTRITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA4</accession>
    <name>Q8TXA4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01985">
        <property type="protein sequence ID" value="AAM01985"/>
        <property type="gene ID" value="MK0771"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0771"/>
    <dbReference type="HOGENOM" id="CLU_448076_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="609" mass="69552" checksum="AB10C9780DC5AD78" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB4</accession>
    <name>PYRH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">MK0760</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXB4"/>
    <dbReference type="SMR" id="Q8TXB4"/>
    <dbReference type="STRING" id="190192.MK0760"/>
    <dbReference type="EnsemblBacteria" id="AAM01974">
        <property type="protein sequence ID" value="AAM01974"/>
        <property type="gene ID" value="MK0760"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0760"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.800"/>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="OMA" id="MGGTHPG"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143918" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <sequence length="223" mass="23774" checksum="D95B95D302514897" modified="2002-06-01" version="1">MYSVVALGGSVVNVDKPERIKETAEILRNGLDSGLKICVVVGGGPTARRYINVARNLGTPETLLDEMGIAVTRLNAMLLGAALGLHDLHVPETPVEAARIVRRNGVAVCGGTHPGHTTDAVAAMIAELLEGPLVIVTNVDGVYDKDPSEPGARKLRELRPEELEELAVRAELKAGGSFVVDPLAAKMISRGQIVTHVVSWEDFRSRGLENVVRGRHNGTIIEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC7</accession>
    <name>DPHB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthine synthase</fullName>
            <ecNumber evidence="1">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dphB</name>
        <name type="ordered locus">MK0747</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXC7"/>
    <dbReference type="SMR" id="Q8TXC7"/>
    <dbReference type="STRING" id="190192.MK0747"/>
    <dbReference type="EnsemblBacteria" id="AAM01961">
        <property type="protein sequence ID" value="AAM01961"/>
        <property type="gene ID" value="MK0747"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0747"/>
    <dbReference type="HOGENOM" id="CLU_066040_0_0_2"/>
    <dbReference type="OMA" id="CTGLEMY"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="PTHR10882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156119" description="Diphthine synthase">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <sequence length="263" mass="29155" checksum="5472A2496B02B643" modified="2002-06-01" version="1">MGELYLVGGGLSDVRDLTLRALEVLASVELVLVDTYTSVYDVSEGDLKRLLNGFGGDPEVRMCSRRDLEERFFDLCEGYDRVALLSPGDPMAATTHVALVVEAADRGWDVEIINGVSVFTAAPSKSGLEMYRFGRTATIPLNVRSVYPYDVLESNRQAGLHTLFLLEVAEDGEFVSVADAARYLLEIEREEGRGVLDPSDLAIAVVRLGFEDELVAWGTLEELSDWEPGEPPQALILPASRLREAEREYIRRVLPHIRDVRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD3</accession>
    <name>Q8TXD3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-bisphosphatase</fullName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">suhB</name>
        <name evidence="4" type="ordered locus">MK0741</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0741"/>
    <dbReference type="EnsemblBacteria" id="AAM01955">
        <property type="protein sequence ID" value="AAM01955"/>
        <property type="gene ID" value="MK0741"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0741"/>
    <dbReference type="HOGENOM" id="CLU_822862_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="337" mass="37087" checksum="068B5175748D7784" modified="2002-06-01" version="1">MRETVEKIVKERVLPEVSKVRRGWVEVGRGADGTPTMRVDEVAEREFLRGLEEEGVDARVISEESGEMRVGEAPEVTLVLDPLDGSHNASRGLPFYCVSVGIADPEAETLDDVEEGLVVSELLTFGPGDVEEKPVVERPLVSAYYYGSDRMPVEFSRGRFKLRCLGSVALELALVGKGALDGFVDVRGSLRPTDVAGAFGAARGELAFIFARNGRELDPSEIPLRPDFRFELATARSEDEARELFEALKEDVGIRVAQRLFGKHPEGLSEHALCGKLGEECCELAHAVGKEEGYGEELADVFALVMNLANELDVDVLTELRRKFRRVFGCSDRRSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE8</accession>
    <name>Q8TXE8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CO dehydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cooC_2</name>
        <name evidence="2" type="ordered locus">MK0726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0726"/>
    <dbReference type="EnsemblBacteria" id="AAM01940">
        <property type="protein sequence ID" value="AAM01940"/>
        <property type="gene ID" value="MK0726"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0726"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.767"/>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="OMA" id="LTMGTVD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="157"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="247" mass="26995" checksum="140ECD169B60CCFB" modified="2002-06-01" version="1">MKVGVVGKGGVGKTTVAAALAKALTDVGYEVLAVDADPDPDLAYSLGLPESPTPIVERRELVRERTGAEPGTTYGPVFKVNPRVSDLPDELAVRVAPGLRLLVVGSVENPGEGCFCPAAALARRLIRYVIADRDESVVVDTDAGLEHFGRRVLESVDWVVVVCEPSVKSMKNARESVRLAREMGIERVALVKNKWREDAPEPDLDVDFELTVPYSDKLVELEMEGRPVWEDPVVRKAGEELARRLTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-21" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI4</accession>
    <name>RAD50_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rad50</name>
        <name type="synonym">sbcC</name>
        <name type="ordered locus">MK0690</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXI4"/>
    <dbReference type="SMR" id="Q8TXI4"/>
    <dbReference type="STRING" id="190192.MK0690"/>
    <dbReference type="EnsemblBacteria" id="AAM01904">
        <property type="protein sequence ID" value="AAM01904"/>
        <property type="gene ID" value="MK0690"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0690"/>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="OMA" id="FELPYSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03240">
        <property type="entry name" value="ABC_Rad50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045171">
        <property type="entry name" value="ABC_Rad50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138655" description="DNA double-strand break repair Rad50 ATPase">
        <location>
            <begin position="1"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="1">
        <location>
            <begin position="387"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="575"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <sequence length="876" mass="103706" checksum="D651E682271FD383" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ3</accession>
    <name>RS7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps7</name>
        <name type="ordered locus">MK0680</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ3"/>
    <dbReference type="SMR" id="Q8TXJ3"/>
    <dbReference type="STRING" id="190192.MK0680"/>
    <dbReference type="EnsemblBacteria" id="AAM01895">
        <property type="protein sequence ID" value="AAM01895"/>
        <property type="gene ID" value="MK0680"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0680"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.719"/>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_S7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_S5/S7_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_S7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000124400" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="197" mass="22723" checksum="C0A6C0A3F10B0288" modified="2002-06-01" version="1">MEGNPYLDRLYEMKVFGKWDPTEVEVRDPGLKDYICLKPMYLPHTGGRHAKKRFAKAEVPIVERLINRVMRTEKNTGKKHLAYNIVKRAFDIIHERTGENPIQVLVQALENAAPREETTTIIYGGISYHEAVDSSPQRRLDIALRLITEGAQQRAFRNPKPIEECLAEEIIAAARYDTECHSIRRKEEIERIAEAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK6</accession>
    <name>Y666_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein MK0666</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0666</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXK6"/>
    <dbReference type="SMR" id="Q8TXK6"/>
    <dbReference type="STRING" id="190192.MK0666"/>
    <dbReference type="EnsemblBacteria" id="AAM01881">
        <property type="protein sequence ID" value="AAM01881"/>
        <property type="gene ID" value="MK0666"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0666"/>
    <dbReference type="HOGENOM" id="CLU_089549_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004959">
        <property type="entry name" value="UCP004959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141704" description="UPF0305 protein MK0666">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <sequence length="142" mass="16721" checksum="35566E683C7C2D7D" modified="2002-06-01" version="1">MLRRDAWRQARFVHPRSYREKILRSLFFALTARINQMRRLDPEEYPEDPIEGYDRFLEIVREYAEDPDYDSPLLLLYESLSAAYAIFLRGEPVHPPGTEFPGVGKVRRTDDCYYCPIKERREDQPGSFCTLCPAEQDPEVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM2</accession>
    <name>Q8TXM2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01855">
        <property type="protein sequence ID" value="AAM01855"/>
        <property type="gene ID" value="MK0640"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0640"/>
    <dbReference type="HOGENOM" id="CLU_1639974_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="161" mass="17343" checksum="5872DE8E32705444" modified="2002-06-01" version="1">MGNLPRPAAWLAALTAVLLAVVGPAHGLTQTNAALNPQPVQGLNVEGASKPTSLVIDNSGNLGAVEAGNPPTQAWTTAPIVATVYTQPKVAKAEMVGGNIDFTVSRHRHHDRYDLQVHRHRDHQREHTDGIDRPADHTDFVPRCAHRHNHTDGLPPARSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN7</accession>
    <name>Q8TXN7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01839">
        <property type="protein sequence ID" value="AAM01839"/>
        <property type="gene ID" value="MK0624"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0624"/>
    <dbReference type="HOGENOM" id="CLU_1140570_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="27712" checksum="990AB7113A6B0CAA" modified="2002-06-01" version="1">MTFVTPRVSPTESDIEVGSWKEIEEIVSSLSSGLVRITTRDHEDIYDCFFIVEGGAVVGAYLGRIRAEELSAEEAVRAIRDVVDSVGFALLDVYELEPELLELIKRVNDECLLKEPFTPTEVEEAAPEVEEPERDELLEKLGVDLDAIYESVEAVLEDYFEEEDPFEEFKRMLRALGTQDARVYLRVKVPEGVDERMLEEVRRDLEHALSEFSIDGVEITPSLKEKETVTLRIRDIMKRIRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ9</accession>
    <name>Q8TXQ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Homolog of ABC-type nitrate/sulfonate/taurine/bicarbonate transport systems, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0601</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0601"/>
    <dbReference type="EnsemblBacteria" id="AAM01816">
        <property type="protein sequence ID" value="AAM01816"/>
        <property type="gene ID" value="MK0601"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0601"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.637"/>
    <dbReference type="HOGENOM" id="CLU_028871_10_4_2"/>
    <dbReference type="OMA" id="YQLKLIP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="35192" checksum="2152C2B55E34EBB0" modified="2002-06-01" version="1">MKKVVALGIVIVAVLGGALGYYYYTTTSNVVRIGYQPSDHSAALFVAMAKKMFEKEGIKVETYEFKAGPPETQALAAGKIDVAYIGCVPAITAYSKGVPIKIVAGVNQEGSAIVVRKDEAGKIKDIKDLKGKRVAELMKGSIQDCMLRTALKRAGLDPDKDVDIVEMKTADAVNALGAKQIDAFIEPEPGPTMAVKKGFGVRLMDTGKIWSHHQCCVLVMRKDFIERHPNLAKKVLKVHVMATKYVQEHPDEAAKITAKQLKVPEEVEKEAMRHVRYSVDLDVDSIKMFARFLKQLGYIKELPDWSDFIDLKLLKEVAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-31" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU6</accession>
    <name>PYRDB_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit</fullName>
            <shortName>DHOD B</shortName>
            <shortName>DHODase B</shortName>
            <shortName>DHOdehase B</shortName>
            <ecNumber>1.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Dihydroorotate oxidase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Orotate reductase (NADH)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrD</name>
        <name type="ordered locus">MK0563</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with NAD(+) as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-dihydroorotate + NAD(+) = H(+) + NADH + orotate</text>
            <dbReference type="Rhea" id="RHEA:13513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.14"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXU6"/>
    <dbReference type="SMR" id="Q8TXU6"/>
    <dbReference type="STRING" id="190192.MK0563"/>
    <dbReference type="EnsemblBacteria" id="AAM01778">
        <property type="protein sequence ID" value="AAM01778"/>
        <property type="gene ID" value="MK0563"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0563"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.598"/>
    <dbReference type="HOGENOM" id="CLU_042042_0_0_2"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004589">
        <property type="term" value="F:orotate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.26.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023359">
        <property type="entry name" value="Dihydro_DH_chainA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000409557" description="Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="193"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="247"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="269"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="318" mass="33274" checksum="50D5D632421C96A0" modified="2002-06-01" version="1">MRPVEVLGKRWAHPIACASGALAAHRPGMENAVLRGAAAIFTKTVTESPREGHPGPVFVDYLDEGYALNAMGLPNPGPDRMVVEIEEFRDEFDVPVYASVAADGPEGFKRLARAFSGVADGLELNVSCPHAGKGYGAELGSDPEAVAEITEAAVRAFDGPVSVKLTPNVDRETLLEVAAAAIDAGAEALTAVNTLGPGLRIDLRTASPVLGAGVGGLSGPALKPIALRVVADLALEFGEEVEIIGVGGIRNGEDVVEFLFAGAKAVQVATAAREKDFGDIAMETSHILKELGYDGPEEAIGAALPEYRERLRRLGWCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV0</accession>
    <name>Q8TXV0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein implicated in ribosomal biogenesis, Nop56p homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sik1</name>
        <name evidence="4" type="ordered locus">MK0559</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0559"/>
    <dbReference type="EnsemblBacteria" id="AAM01774">
        <property type="protein sequence ID" value="AAM01774"/>
        <property type="gene ID" value="MK0559"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0559"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.594"/>
    <dbReference type="HOGENOM" id="CLU_015495_1_0_2"/>
    <dbReference type="OMA" id="REWYSLH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D RNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="PTHR10894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="SSF89124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nop" evidence="3">
        <location>
            <begin position="264"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="376"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="420" mass="48515" checksum="D142F8A6E8BAAA8E" modified="2002-06-01" version="1">MGVYVAENFTGVYAFDEEGNLIDHEPFPKDPDEIVERLLKRERGEVLEEEEALLSRLDADVINFEGTKADRERLEEVFDGELVVEFPNVAGEVLRERARELAVEVGVVDSEEEYSELVYEVGMKLSKEKVRATVEERDQMIIQAINTIDDIDRILNILTDRVREWYGIHFPEINKIVKKHDDFVTLVAELGHRKNFTYDNIKEVLPEFPDHLAEKLEEAAKDSMGAEMDEKDLAAVQRIAEVARELYEIRRKTADYIDESMDDVAPNVKALVGPLIGARLIALAGGLKEMAKLPASTIQLLGAEKALFRHLTKGTKPPKHGVIFQHPLIHRSPWWQRGKIARALAGKLAIAARIDAYSGEYRGDELRRQLEQRVKEIKEKYPKPPKRKRGGRPPRGRRPRRRRSPRRSRRDRRRRSRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY2</accession>
    <name>Q8TXY2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-13 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01742">
        <property type="protein sequence ID" value="AAM01742"/>
        <property type="gene ID" value="MK0527"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0527"/>
    <dbReference type="HOGENOM" id="CLU_380663_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="74"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="727" mass="84306" checksum="82FD2A7E55E6F63C" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY07</accession>
    <name>FAU1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribonuclease FAU-1</fullName>
            <ecNumber evidence="1">3.1.26.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA-binding protein FAU-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fau-1</name>
        <name type="ordered locus">MK0499</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable RNase involved in rRNA stability through maturation and/or degradation of precursor rRNAs. Binds to RNA in loop regions with AU-rich sequences.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAU-1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY07"/>
    <dbReference type="SMR" id="Q8TY07"/>
    <dbReference type="STRING" id="190192.MK0499"/>
    <dbReference type="EnsemblBacteria" id="AAM01714">
        <property type="protein sequence ID" value="AAM01714"/>
        <property type="gene ID" value="MK0499"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0499"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.529"/>
    <dbReference type="HOGENOM" id="CLU_044303_0_0_2"/>
    <dbReference type="OMA" id="GTYVNVC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016891">
        <property type="term" value="F:endoribonuclease activity, producing 5'-phosphomonoesters"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01910">
        <property type="entry name" value="RNA_binding_AU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007295">
        <property type="entry name" value="DUF402"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035930">
        <property type="entry name" value="FomD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016730">
        <property type="entry name" value="RNA-bd_FAU-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04167">
        <property type="entry name" value="DUF402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018644">
        <property type="entry name" value="RNA-binding_FAU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159234">
        <property type="entry name" value="SSF159234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000334200" description="Probable ribonuclease FAU-1">
        <location>
            <begin position="1"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="92"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01910"/>
        </source>
    </evidence>
    <sequence length="484" mass="54156" checksum="076EDE9AB5857D9F" modified="2002-06-01" version="1">MPCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGIEIQGPADDVRELVDILQSEVWATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGFLSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEYAVLVPVEGIRVSRKIRDERERERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIRDVREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVVAERLIRCLTRSEGPSEGDRIDIVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPGFYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGRYYNIGTPLEVFKNCVRYVDLEVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKGFEHG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-06" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY29</accession>
    <name>EHAA_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ehaA</name>
        <name type="ordered locus">MK0477</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the integral membrane subunits of multisubunit membrane-bound [NiFe]-hydrogenase eha. Eha is predicted to form large electron transfer complex and might catalyze energy-driven reduction of low-potential redox carriers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Putative multisubunit membrane-bound [NiFe]-hydrogenase eha is composed of at least 20 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EhaA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY29"/>
    <dbReference type="STRING" id="190192.MK0477"/>
    <dbReference type="EnsemblBacteria" id="AAM01692">
        <property type="protein sequence ID" value="AAM01692"/>
        <property type="gene ID" value="MK0477"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0477"/>
    <dbReference type="HOGENOM" id="CLU_174516_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011306">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17367">
        <property type="entry name" value="NiFe_hyd_3_EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005019">
        <property type="entry name" value="EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000138114" description="Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="97" mass="9898" checksum="39F323FC2DB8522F" modified="2002-06-01" version="1">MKDPGLVAVGVAAAVAFGTALALGLPPIQRDKPRRKSWEVSAAFPTPVIAAGATVLVIRVIGYHPPIPLAIVGAVVGALSAAFTAYIEKVFPRPEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY45</accession>
    <name>Q8TY45_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehbK</name>
        <name evidence="2" type="ordered locus">MK0461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01676">
        <property type="protein sequence ID" value="AAM01676"/>
        <property type="gene ID" value="MK0461"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0461"/>
    <dbReference type="HOGENOM" id="CLU_061721_0_0_2"/>
    <dbReference type="OMA" id="IWYLYEF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="200"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="233"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="379" mass="43081" checksum="D037AF209273C20B" modified="2002-06-01" version="1">MVVDLHFSFEDRLRNVTINIVEVERPDECAGCGLCAEVCPTGAIEVDERVRLDEDRCVACSFCVQACPRDVFRFYEVSFTELKPKRRPVRVPKADIEVRFIGVDLRTCDRCENRPCIEVCPTGVMREIIEEHRIDLDACHGCLECVKVCPYGSVTVELEVPQLKRRSNPRLNRELCVECNRCHEVCPTGAADNVPDGDPDPERCLGCYNCVAYCPTEALKRPDHRPRPKCTDEVFYIQPDMCIGCRICYDVCPVDAIRIEEITRMPVIMPDLCVRCGLCADACPTSAVDRVPTEEAEREVLRSRISDAFLGILTREMLEAAEEFGSTTRTERDVEEKLSELLERKMSEEMIRRVIEFEVKNVIEELMAEVVSGRDSRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY47</accession>
    <name>Q8TY47_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0459"/>
    <dbReference type="EnsemblBacteria" id="AAM01674">
        <property type="protein sequence ID" value="AAM01674"/>
        <property type="gene ID" value="MK0459"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0459"/>
    <dbReference type="HOGENOM" id="CLU_097041_0_0_2"/>
    <dbReference type="OMA" id="CVKCEIC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="28224" checksum="7F33638B8F8E0E27" modified="2002-06-01" version="1">MKTLEISIDKVVEMVLDRVEDADEDTVLEVLSETPAVRREFVTIDPERCVGCKTCYEECPVDALTEPDSTNPPEVDHDACVRCRLCAKSCPVDAIKVVSGEARVTKDSIEVKLEEVDVIRRKFVLRKAILRKDRCIACRLCEQICPVEAPNIDKLRIDEDKCIGCKACEHACPVDAIVIERTLTPPEFEREIELDQDMCIGCEVCVEVCPVDAVEMEGDVANISYDRCIRCGECARNCPTGAIKIKEVREEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE4</accession>
    <name>Q8TYE4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01571">
        <property type="protein sequence ID" value="AAM01571"/>
        <property type="gene ID" value="MK0356"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0356"/>
    <dbReference type="HOGENOM" id="CLU_2204132_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="107" mass="10946" checksum="1A8857F6DB8ECA2F" modified="2002-06-01" version="1">MAISEIILAGLSAGVAIEFAMPAMIRRSLPLRLAVILGVIAAASCSLSILWPILRPVGAVSGLLTGILALAVRAEELKSGHFGAAGFSVRGAASYMLLISLGIYALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE9</accession>
    <name>Q8TYE9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted DNA-binding protein containing a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0351"/>
    <dbReference type="EnsemblBacteria" id="AAM01566">
        <property type="protein sequence ID" value="AAM01566"/>
        <property type="gene ID" value="MK0351"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0351"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.372"/>
    <dbReference type="HOGENOM" id="CLU_065511_0_0_2"/>
    <dbReference type="OMA" id="NKTQNCV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017674">
        <property type="entry name" value="Methan_mark_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03280">
        <property type="entry name" value="methan_mark_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1743" evidence="1">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="315" mass="35058" checksum="84DCF87EAED42B1B" modified="2002-06-01" version="1">MVSLSPEEVRRKCSEKGWVTMYERIATLTDEDRERVLLIEDHPTPVGAEWVVRNYEATSPLVEKAWREGKRHFFLLRVGEASLDLEPSVRAAGVESVEVRDGEVRVTHAGLAGAGVGAALSRGCAEGVSRVEIHEEGGGSRLGRATVVTPELRRLVIGVDDTDTEEEGATWSAVDVICRRLEDEAGVFYSRHVTVQLYPKTPHRTRNCAAVVVELGVPTKRVERVKREFLRMLKEVSFSDHTCAAFWDRLEFPRELRDLGNAAKRRIVSREEVDEVVEICEIEVVSIGDGERGRIGAVAALPFIDDHGLAARVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH8</accession>
    <name>Q8TYH8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Selenocysteine-containing anaerobic dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0321</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0321"/>
    <dbReference type="KEGG" id="mka:MK0321"/>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_2"/>
    <dbReference type="OMA" id="DGPPCCY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0711">Selenium</keyword>
    <keyword evidence="8" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="7">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="8">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="685" mass="77203" checksum="6A92753C1BFF7DCB" modified="2008-02-26" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI6</accession>
    <name>Q8TYI6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01528">
        <property type="protein sequence ID" value="AAM01528"/>
        <property type="gene ID" value="MK0311"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0311"/>
    <dbReference type="HOGENOM" id="CLU_3163120_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="47" mass="4698" checksum="EC481C09C88D04CF" modified="2002-06-01" version="1">MGRAPVLVYRGAGHVALHVVRGGAAAGWGIVHAGEPVTIIPGVVAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ7</accession>
    <name>Q8TYJ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01517">
        <property type="protein sequence ID" value="AAM01517"/>
        <property type="gene ID" value="MK0300"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0300"/>
    <dbReference type="HOGENOM" id="CLU_1017845_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="273" mass="30466" checksum="15BCCC0C6B211B2E" modified="2002-06-01" version="1">MLGPLIALVSVVPTGAAADHVTDVTSKVNVDGDYEACKDLLVGVEVYDDQGYVIASCCWDGRCDALGETKVSENHLSRTVDRFIVKMTVRYKNQLLLDERVFIRVIPGQGVIIEFNGRTIELRSANGTWGPEEPLRVVRTIDGKTVGITFRPSAKVEHAEQLSNQWVVDFLYSIDGDVIVHDKKGDYHITFQAHVKHTPLKNAEHDYMQTCPYGKKKTVDEELTHGTFGHLVDDHATKIEDLTRAWANEKMPVNPLLPALAAVLSLLGIRREY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK3</accession>
    <name>Q8TYK3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2008-01" db="PDB data bank">
            <title>Crystal structure of protein MK0293 from Methanopyrus kandleri AV19.</title>
            <authorList>
                <person name="Patskovsky Y."/>
                <person name="Romero R."/>
                <person name="Bonanno J.B."/>
                <person name="Malashkevich V."/>
                <person name="Dickey M."/>
                <person name="Chang S."/>
                <person name="Koss J."/>
                <person name="Bain K."/>
                <person name="Wasserman S.R."/>
                <person name="Sauder J.M."/>
                <person name="Burley S.K."/>
                <person name="Almo S.C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 2-176</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2019" name="Nat. Commun." volume="10" first="452" last="452">
            <title>A five-residue motif for the design of domain swapping in proteins.</title>
            <authorList>
                <person name="Nandwani N."/>
                <person name="Surana P."/>
                <person name="Negi H."/>
                <person name="Mascarenhas N.M."/>
                <person name="Udgaonkar J.B."/>
                <person name="Das R."/>
                <person name="Gosavi S."/>
            </authorList>
            <dbReference type="PubMed" id="30692525"/>
            <dbReference type="DOI" id="10.1038/s41467-019-08295-x"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 98-176</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3C19">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=2-176"/>
    </dbReference>
    <dbReference type="PDB" id="6IWJ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A/B=98-176"/>
    </dbReference>
    <dbReference type="PDBsum" id="3C19"/>
    <dbReference type="PDBsum" id="6IWJ"/>
    <dbReference type="SMR" id="Q8TYK3"/>
    <dbReference type="STRING" id="190192.MK0293"/>
    <dbReference type="EnsemblBacteria" id="AAM01510">
        <property type="protein sequence ID" value="AAM01510"/>
        <property type="gene ID" value="MK0293"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0293"/>
    <dbReference type="HOGENOM" id="CLU_1521878_0_0_2"/>
    <dbReference type="EvolutionaryTrace" id="Q8TYK3"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="PTHR36566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="DUF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0533">Nickel</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3C19"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="6IWJ"/>
        </source>
    </evidence>
    <sequence length="176" mass="19551" checksum="BFD4037D9F071C5F" modified="2002-06-01" version="1">MGLDYEPSHLMFLVTVLDDRDGEVLGDAIQKLIEREEVLACHAVPCVTKKNRPGHVLVVLVDGGEDPDRVAEDVARDIMVLTGSTGVDRFDADGVYSVPSRFEDVRVVYGEREWRVSVKIAETEEGEVVTVKAEFDECREIGEETGIPPREVKAMVEAAARVGGWVDLKEREIKVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP6</accession>
    <name>EF1A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 1-alpha</fullName>
            <shortName evidence="2">EF-1-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">MK0247</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYP6"/>
    <dbReference type="SMR" id="Q8TYP6"/>
    <dbReference type="STRING" id="190192.MK0247"/>
    <dbReference type="EnsemblBacteria" id="AAM01464">
        <property type="protein sequence ID" value="AAM01464"/>
        <property type="gene ID" value="MK0247"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0247"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.249"/>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090981" description="Elongation factor 1-alpha">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="423" mass="46652" checksum="02E3838D8A44B3D5" modified="2002-06-01" version="1">MAKEKEHINLAFIGHVDHGKSTLVGRLLYDTGVIEDKDLGEGEDKFRVIMDTLEEERERGVTIDLAHTKFETDNYEFTIVDCPGHRDFVKNMITGASQADAAILVVAADDGVMPQTKEHAFLAKTLGIDQLIVAINKMDLVDYDENRYEEVKQEVAELLKTIGYNVDEIPFIPISAFEGDNVVEKSDNTPWYDGPTLLEALDNLEPPEKPTDKPLRIPIQDVYSITGVGTVPVGRVETGVLEVGDTVRFEPAYTATGGRKGEGEVRSIEMHHEEIERAEPGDNIGFNVKGVGKNDISRGDVACHPDEPATVVTPDDTFIAQIVVLQHPSAITAGYTPVFHCHTAQVACKFEELIEKIDPATGEVIEENPDFLKTGEAAKVRIRPTKPMVIEEVSFIPQLGRFAIRDMGQTVAAGMCVKIEKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS1</accession>
    <name>RL37_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L37e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37e</name>
        <name type="ordered locus">MK0221</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYS1"/>
    <dbReference type="SMR" id="Q8TYS1"/>
    <dbReference type="STRING" id="190192.MK0221"/>
    <dbReference type="EnsemblBacteria" id="AAM01438">
        <property type="protein sequence ID" value="AAM01438"/>
        <property type="gene ID" value="MK0221"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0221"/>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="OMA" id="RIRRYSW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_L37e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139730" description="50S ribosomal protein L37e">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <sequence length="62" mass="7500" checksum="17690750ACBA2AA8" modified="2002-06-01" version="1">MAKGTPSFGKRNKTKTHVRCRRCGRRAYHVRKGYCAACGFGRSRRIRRYSWQNKKVNRKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS7</accession>
    <name>Q8TYS7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01432">
        <property type="protein sequence ID" value="AAM01432"/>
        <property type="gene ID" value="MK0215"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0215"/>
    <dbReference type="HOGENOM" id="CLU_938798_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="296" mass="32078" checksum="5FC5C8A123866BA7" modified="2002-06-01" version="1">MDLAGGWHVGAAFVTVATLTTVLLALLTYSIGVVGRWIKAPIATTLGAAIIGAGVGLFLLAPMLSDIPKVVAGLEKNGYLTVELSTAPSDLPKALEAVKKYRGKPVGRYLELEVAMSEPVPSDRRRWFESKVPKVFPGVKDVRFSSPTKMVVRMDLKSVPPSKVSQLADELSGWITYTSGFVVVGVTATIDVEVPPSEYADLKGEIREIADLRVIYDPTKAAQRRIARMLPSPRTTVIASTLAFAGIAVIGWYGISHIIGPLTSPLRRSAEGRRLVRREKGRIRRETPGLPPRGKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-19" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT6</accession>
    <name>Y206_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0280 protein MK0206</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0206</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0280 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYT6"/>
    <dbReference type="SMR" id="Q8TYT6"/>
    <dbReference type="STRING" id="190192.MK0206"/>
    <dbReference type="EnsemblBacteria" id="AAM01423">
        <property type="protein sequence ID" value="AAM01423"/>
        <property type="gene ID" value="MK0206"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0206"/>
    <dbReference type="HOGENOM" id="CLU_074757_0_0_2"/>
    <dbReference type="OMA" id="YGVCTSS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01079">
        <property type="entry name" value="UPF0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037456">
        <property type="entry name" value="MA1715-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007183">
        <property type="entry name" value="UPF0280"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006421">
        <property type="entry name" value="UCP006421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140487" description="UPF0280 protein MK0206">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01079"/>
        </source>
    </evidence>
    <sequence length="241" mass="25166" checksum="B7B7809595D58EE2" modified="2002-06-01" version="1">MYSMRVVIEETDVTIRADSKESVSSAAKAVKLHRSELDRYVAKDPAFVTAKVPVRTLEGAPEVAKLMSRAAEPFGVGPMAAVAGAIAELAARASEPTVIVDNGGDVQVRARRSVVVGLYVSDDHPLSGRIGFEIEGVLGVCTSSGKFGHSYSAGKADAVTVFAERASLADAAATAICNLTSGDDPEAAVQRALEFADDFTGDLIEAAVVIRGDFVGISGRPPKIVSLRGGRIKPARLEPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU6</accession>
    <name>Q8TYU6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0195"/>
    <dbReference type="DNASU" id="1477498"/>
    <dbReference type="EnsemblBacteria" id="AAM01412">
        <property type="protein sequence ID" value="AAM01412"/>
        <property type="gene ID" value="MK0195"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0195"/>
    <dbReference type="HOGENOM" id="CLU_1113872_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="27712" checksum="F02D91930E4BCDD5" modified="2002-06-01" version="1">MLSGDETLTVYLARVLSCPELFRVSTPEEARRIAEKILSGEIEPPLEFFGLRRDAVNEVLAVTDGPAGENVAPVGLRVRGDSIVVNLYPGSRTYENFVRTEELTACIVPDPIRFLKALSKELAIETVGDGTKVAEGTRAYLELEAKEIHEGKPLTAELQVVGWGLLHPRPRALVRGESALLEALVELTRIHLDEDHVDACKRALEVVKRTIWSEEYQWAVEKVERELRGKEDGPDHQDTSPRIRRATGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV8</accession>
    <name>Q8TYV8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein, putative amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0183</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0183"/>
    <dbReference type="EnsemblBacteria" id="AAM01400">
        <property type="protein sequence ID" value="AAM01400"/>
        <property type="gene ID" value="MK0183"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0183"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.183"/>
    <dbReference type="HOGENOM" id="CLU_055029_2_1_2"/>
    <dbReference type="OMA" id="VEWHGPH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="PTHR35005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="SSF102215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="25103" checksum="1E434D61E99FCF96" modified="2002-06-01" version="1">METRKLRHPEVDLEGEVVLLPVGSTEQHGLHLPLGTDHLIAEALCREVSKRTGAPWYPAIPYGVSRHHMGFPGTVSLRTKTMVALLTDVHRSFLHHGAAATLAVNGHGGNEAALGTVAEEEERFHWISWWKLAPIDELETDWGGHADELETSVMLYLHPELVGEERKVDGRPTKPWEFPDYHEISETGTKGDPRPATADKGKRIFKTVVERLVDIVEELREMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW7</accession>
    <name>Q8TYW7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0174"/>
    <dbReference type="EnsemblBacteria" id="AAM01391">
        <property type="protein sequence ID" value="AAM01391"/>
        <property type="gene ID" value="MK0174"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0174"/>
    <dbReference type="HOGENOM" id="CLU_102155_0_0_2"/>
    <dbReference type="OMA" id="DRVSAYH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5616" evidence="1">
        <location>
            <begin position="65"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="227" mass="25097" checksum="B7B05800516AAB47" modified="2002-06-01" version="1">MDSIEKYLLVTRRMVNAGIPKDRALNTVQRTWGLTNREKKALYRIVWSRLESLLRAGKRVPTPLLRGQDLVIDGYNVLVGLASLDAGEAVLCDDDVVRDLRMSPKLEEEEVQTALEMLETYLRRVEPRSVRILFDAPVSGSGELAARVERYLKDSLNVPVRASAVKGVDEKLVRAQGVPVTSDSGIIDRVSAYHDAVREVAAAEGIAVWIPPGPSEPKFVRALVPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ0</accession>
    <name>Q8TYZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0151"/>
    <dbReference type="EnsemblBacteria" id="AAM01368">
        <property type="protein sequence ID" value="AAM01368"/>
        <property type="gene ID" value="MK0151"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0151"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.151"/>
    <dbReference type="HOGENOM" id="CLU_013458_2_1_2"/>
    <dbReference type="OMA" id="DWPLVGI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="TsaA-like" evidence="4">
        <location>
            <begin position="27"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00033753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="19543" checksum="B07A1BC7CC24012E" modified="2002-06-01" version="1">MSSPDTPSCAHTSPPRHPPGDDVPLRLTPIGWVVERTDEEGLVKVRERYRKCLEGLEGFSHVWILWWGHEADRTVTRVRPVHGEVPELVGVFACRSPDRPNPVLLTLCRILEVRPNSGELRVSGLDARAGSPVVDMKPYLPGYDEPDGEVSVPEWVEMVKRGHRPPRTRGHRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-01-11" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ5</accession>
    <name>TMCA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Met) cytidine acetyltransferase TmcA</fullName>
            <ecNumber evidence="1">2.3.1.193</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tmcA</name>
        <name type="ordered locus">MK0146</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + ATP + cytidine(34) in elongator tRNA(Met) + H2O = ADP + CoA + H(+) + N(4)-acetylcytidine(34) in elongator tRNA(Met) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43788"/>
            <dbReference type="Rhea" id="RHEA-COMP:10693"/>
            <dbReference type="Rhea" id="RHEA-COMP:10694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:74900"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.3.1.193"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA cytidine acetyltransferase family. TmcA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.193" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYZ5"/>
    <dbReference type="SMR" id="Q8TYZ5"/>
    <dbReference type="STRING" id="190192.MK0146"/>
    <dbReference type="PRIDE" id="Q8TYZ5"/>
    <dbReference type="EnsemblBacteria" id="AAM01363">
        <property type="protein sequence ID" value="AAM01363"/>
        <property type="gene ID" value="MK0146"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0146"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.146"/>
    <dbReference type="HOGENOM" id="CLU_004652_1_0_2"/>
    <dbReference type="OMA" id="NEPDDLG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051392">
        <property type="term" value="F:tRNA N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01886">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="Helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024914">
        <property type="entry name" value="tRNA_acetyltr_TmcA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="PTHR10925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="DUF1726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="Helicase_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000403131" description="tRNA(Met) cytidine acetyltransferase TmcA">
        <location>
            <begin position="1"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="480"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="590"/>
            <end position="592"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="597"/>
            <end position="603"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="630"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="637"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01886"/>
        </source>
    </evidence>
    <sequence length="855" mass="98136" checksum="D3A95DFEB6903278" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ00</accession>
    <name>Q8TZ00_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0141</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01358">
        <property type="protein sequence ID" value="AAM01358"/>
        <property type="gene ID" value="MK0141"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0141"/>
    <dbReference type="HOGENOM" id="CLU_045498_5_3_2"/>
    <dbReference type="OMA" id="MYHHIIN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="263" mass="27116" checksum="41E9CDC09E325A0B" modified="2002-06-01" version="1">MPGLTALAVLELLGTGFIAGYLGGIIGTGGCVLMLPMLVFLLKYPIPEAIATTVFAVVWTATFGTMSHAKLGNIDYETSAIVLAAGAIGALLGSVIFALIMKHTGALQVILGAAFLYAAVRMIYEWIKKIPGSEADEIPGKPSSKAAIGFGIGILTGILGLGGGYALVPSFIYLLDAPVHLAVGTSMISMIPMATVSAAYKMAQGLTDLVGGTLLGLGTIAGVKLGAKTTQKIKPWTIKGIFGVVFLYISLKFILQGLGIKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ24</accession>
    <name>AROE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">MK0117</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S65949">
        <property type="entry name" value="S65949"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ24"/>
    <dbReference type="SMR" id="Q8TZ24"/>
    <dbReference type="STRING" id="190192.MK0117"/>
    <dbReference type="EnsemblBacteria" id="AAM01334">
        <property type="protein sequence ID" value="AAM01334"/>
        <property type="gene ID" value="MK0117"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0117"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.116"/>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136061" description="Shikimate dehydrogenase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="160"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <sequence length="290" mass="31413" checksum="DA8DF9AEB739BBA0" modified="2002-06-01" version="1">MSVSVSVDAETNVVGLIGHPVEHSLSPAMHNAAFKELGLNYVYLAFDVPPERLEGAVRGAADLGIVGLNVTIPHKEAVMELCDELDRDAELIGAVNTVRFSRGKIEGFNTDGEGFLRALREETYFDPRGTKSVILGAGGAARAVSFKLATEGADEIVIANRTVDRAERLAEELKEKVGVKARAIGLDGDEIERELRDADLLVDATPVGMYPNEDEPPLVTADQMHEDLIVNDLVYNPPRTRLLEEAEKAGATPVSGVGMLVYQGALAFELWTGEEAPVEVMREAVLEHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ27</accession>
    <name>Q8TZ27_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted sugar kinase of the RNAseH/HSP70 fold</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0113"/>
    <dbReference type="EnsemblBacteria" id="AAM01330">
        <property type="protein sequence ID" value="AAM01330"/>
        <property type="gene ID" value="MK0113"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0113"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.112"/>
    <dbReference type="HOGENOM" id="CLU_060932_0_0_2"/>
    <dbReference type="OMA" id="TDCFADR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002756">
        <property type="entry name" value="MfnF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03123">
        <property type="entry name" value="one_C_unchar_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="70"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="345" mass="37669" checksum="5FA7D12C6A17289D" modified="2002-06-01" version="1">MGCECIGGNVVKALGLDVGGAHTDAALVRYDEDGKVMVLGTDRVYLPMWKKKKRLKKTIKRIVHKFKPDVVGLTMTGELADAFNTRREGVEYIVRTVTSACHAPVYVVTSDGSTVPPEEALRRWREVASANWRATAEVLAHVRPGSYLLVDLGSTTLDLIPIIRGEVAAEGRTDLERMKNGELAYLGALRTPISFLLREVEIDGEPVPVSYEYFSIVADALLLLGEIDPEDYTPETPDGRGKSPEECARRLARTVCSDPEELGWEGVMDLAKTAVRALLGQLLKHIELKLQEHGLDTVVAAGAGDFLIEMACKRIGVEVEPFDEIFGKGSEVAPAVGAAFLAIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ77</accession>
    <name>Q8TZ77_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0062"/>
    <dbReference type="EnsemblBacteria" id="AAM01279">
        <property type="protein sequence ID" value="AAM01279"/>
        <property type="gene ID" value="MK0062"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0062"/>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="37"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="22528" checksum="AF47994E5D81A267" modified="2002-06-01" version="1">MIDRRLAMELDGLEGFPEPKLSLEQYETPGEIVRVLLSIADREFGLECSRVLDLGAGTGRIGIGAALAGACEVTCVEVDSKAVEVARRNVKRAGVEDRVEVVEADVRDFEPEDQYDVTIMNPPFGAQRRGADRPFVEVALEASSGVVSLHRAGTEEFWKRRARELGATCDAIGVVRFPIPAMYPHHRSRIRHVDAVVLVFKKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ80</accession>
    <name>Q8TZ80_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0059"/>
    <dbReference type="EnsemblBacteria" id="AAM01276">
        <property type="protein sequence ID" value="AAM01276"/>
        <property type="gene ID" value="MK0059"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0059"/>
    <dbReference type="HOGENOM" id="CLU_1222532_0_0_2"/>
    <dbReference type="OMA" id="THVYVQL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019202">
        <property type="entry name" value="DUF2067"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09840">
        <property type="entry name" value="DUF2067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="226" mass="26151" checksum="30B3944A64FC6B1A" modified="2002-06-01" version="1">MPKMEKREITFTFPDRDSAQRFLRAVEATQARGVDTVVELRGTRVKVKVFGPHAAVKAHIRKLGELRRTVLSESEEEKQVRLHLSTICREAGAPKIPSELLRDALRRRGYRVRVRGPWITTNAPMSELREFVRELAEAYREARFYAASEPVRRMLAILSHEYDVDPMDLAYEAIERGVLREGEDGRCELREDPKSTERKLEEIASELRKARKGRGKTLEDFLHEPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-07" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8X260</accession>
    <accession>Q7LWW2</accession>
    <name>NFI_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease V</fullName>
            <ecNumber evidence="1">3.1.21.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxyinosine 3'endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Deoxyribonuclease V</fullName>
            <shortName evidence="1">DNase V</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nfi</name>
        <name type="ordered locus">MK1435</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="6015" last="6020">
            <title>A type IB topoisomerase with DNA repair activities.</title>
            <authorList>
                <person name="Belova G.I."/>
                <person name="Prasad R."/>
                <person name="Kozyavkin S.A."/>
                <person name="Lake J.A."/>
                <person name="Wilson S.H."/>
                <person name="Slesarev A.I."/>
            </authorList>
            <dbReference type="PubMed" id="11353838"/>
            <dbReference type="DOI" id="10.1073/pnas.111040498"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.</text>
            <dbReference type="EC" id="3.1.21.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endonuclease V family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.7" evidence="1"/>
    <dbReference type="EMBL" id="AF311944">
        <property type="protein sequence ID" value="AAL61958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8X260"/>
    <dbReference type="SMR" id="Q8X260"/>
    <dbReference type="EnsemblBacteria" id="AAM02648">
        <property type="protein sequence ID" value="AAM02648"/>
        <property type="gene ID" value="MK1435"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1435"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1591"/>
    <dbReference type="HOGENOM" id="CLU_047631_1_1_2"/>
    <dbReference type="OMA" id="RIHFRQA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043737">
        <property type="term" value="F:deoxyribonuclease V activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06559">
        <property type="entry name" value="Endonuclease_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00801">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007581">
        <property type="entry name" value="Endonuclease-V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28511">
        <property type="entry name" value="PTHR28511"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04493">
        <property type="entry name" value="Endonuclease_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159688" description="Endonuclease V">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with target DNA" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00801"/>
        </source>
    </evidence>
    <sequence length="229" mass="25331" checksum="74881C35F5FAF924" modified="2002-03-01" version="1">MKSAEWFEASTEEERVEIQRKVARKVRLEPLDDVDAVAGVDVSYRGEEYRAAAVVLDPETYEVLDRRVVHGTTDVPYEPGFLAFREGPPALEALEGLDFDLLFVHGHGVAHPRRAGLASHLGVALDVPTIGVARRPLVGRSKEEPSRIGDTTPLVHRGEVVGYLVRTDAEARPVVVSPGHRCNLEDAVRWTLRLVRVGKWPEPLRLADLLSRRGASRVEGESRGAGVRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>P58835</accession>
    <name>HIS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName>PRA-PH</shortName>
            <ecNumber>3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisE</name>
        <name type="synonym">hisI_1</name>
        <name type="ordered locus">MK0500</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58835"/>
    <dbReference type="SMR" id="P58835"/>
    <dbReference type="STRING" id="190192.MK0500"/>
    <dbReference type="EnsemblBacteria" id="AAM01715">
        <property type="protein sequence ID" value="AAM01715"/>
        <property type="gene ID" value="MK0500"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0500"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.530"/>
    <dbReference type="HOGENOM" id="CLU_123337_0_0_2"/>
    <dbReference type="OMA" id="FTHEKGE"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136392" description="Phosphoribosyl-ATP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="100" mass="11457" checksum="3863DBB84008C6DB" modified="2002-05-27" version="1">MGDPEVLLEVYEVIRNRIEERPEGSYVAELTEDDDTKPAINKICEKIIEESGELILAAKDGDREGVVYESTDLIFHVLVLLAYLGIEIGEVFDEFERRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY3</accession>
    <name>Q8TGY3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1539"/>
    <dbReference type="EnsemblBacteria" id="AAM02752">
        <property type="protein sequence ID" value="AAM02752"/>
        <property type="gene ID" value="MK1539"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1539"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1700"/>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_2"/>
    <dbReference type="OMA" id="ARDERCV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="5">
        <location>
            <begin position="175"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="266"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="450" mass="51125" checksum="896B2E6CA92D71A3" modified="2002-06-01" version="1">MTSRDAKTYEEAVEIVLRTLVKAERIKPTQYARRLVMKEMNPSREARSVSAGLLYSVLQKRGLLDEVIEDVLEITDDVTELDTWVRNAARIAVNEVVFEDGDPDEVADVLHGLVRRLANPGAAAIVKAFTLDLKDYEPPEPEDELDRLKWEYYHPRWLIERWMEMFGDPDEVVALLEANNRRPPLTIRVNTLKVDPEELAERLQRKYRVTVEPGRFLDEILKIPEGLPIGEMPEWEEGLFVIQDEAAALASAVLNPKPGEVVVDLCAAPGGKTTHMAQLMGGEGKIVAIDVDEVRMERLREIAERMGVLDCIETHLMDGREAPEKLGREFADAVLVDPPCSADGTIPKNPERRWRITPDELERLPKFQYELLKAGAEMVKPGGRLLYSTCSMFPEEDEEVVRRFLDEHPEFELLEVKVGDQGFDMPEACRLFPHRHETCGFFIALMGRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ2</accession>
    <name>Q8TGZ2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA (cytosine(72)-C(5))-methyltransferase</fullName>
            <shortName evidence="4">tRNA:m(5)C72 MTase</shortName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0769</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in several tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(72) in tRNA + S-adenosyl-L-methionine = 5-methylcytidine(72) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:61988"/>
            <dbReference type="Rhea" id="RHEA-COMP:15996"/>
            <dbReference type="Rhea" id="RHEA-COMP:15997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0769"/>
    <dbReference type="EnsemblBacteria" id="AAM01983">
        <property type="protein sequence ID" value="AAM01983"/>
        <property type="gene ID" value="MK0769"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0769"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.809"/>
    <dbReference type="HOGENOM" id="CLU_005316_1_0_2"/>
    <dbReference type="OMA" id="WHFHESE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02237">
        <property type="entry name" value="NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043699">
        <property type="entry name" value="NSUN6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="85"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4 5">
        <location>
            <begin position="179"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="39261" checksum="AA22AA3AA1B55C08" modified="2002-06-01" version="1">MAALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEGNHHVVVADKFAAESVYVGADLYAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGREMEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPSILAVEALSPEPGETVVDLCAAPGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRGTADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTLTPTENELVVERAIRELNLEPETPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT2</accession>
    <name>COBS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="1">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobS</name>
        <name type="ordered locus">MK1671</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUT2"/>
    <dbReference type="STRING" id="190192.MK1671"/>
    <dbReference type="EnsemblBacteria" id="AAM02884">
        <property type="protein sequence ID" value="AAM02884"/>
        <property type="gene ID" value="MK1671"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1671"/>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000146911" description="Adenosylcobinamide-GDP ribazoletransferase">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <sequence length="241" mass="25264" checksum="E1C25302FBF7F6B6" modified="2002-06-01" version="1">MIRVEFLKVFRFLTVLPIGEHPKSPREIGEQAWLGLPAVGLVSGLLAGVVAWAFAGTPVRGCLVVLTLLVLEGAQHFDGLVDVGDALMAGVISEEGATKAMRDPRVGVGGLAIGSMALLLAVASFGWIPFEVLVPIEVFSRFTVLPMAAVGEPAPASYSGRVFTEYVDADQVLLGGILSTVVSLPFSPVATLTCAVCSAVVAWTCLEAARRTIRGVNGDFLGASIWVSRVLSAVCLSSLPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT6</accession>
    <name>KPRS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="1">RPPK</shortName>
            <ecNumber evidence="1">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="1">P-Rib-PP synthase</shortName>
            <shortName evidence="1">PRPP synthase</shortName>
            <shortName evidence="1">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prs</name>
        <name type="synonym">prsA</name>
        <name type="ordered locus">MK1667</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUT6"/>
    <dbReference type="SMR" id="Q8TUT6"/>
    <dbReference type="STRING" id="190192.MK1667"/>
    <dbReference type="EnsemblBacteria" id="AAM02880">
        <property type="protein sequence ID" value="AAM02880"/>
        <property type="gene ID" value="MK1667"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1667"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1831"/>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141239" description="Ribose-phosphate pyrophosphokinase">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <sequence length="291" mass="31382" checksum="395480BCC450C8B5" modified="2002-06-01" version="1">MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQDENLVKAIITLDAARENGAEEVIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALITVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLVVIGPDEGARELAREVASICGVEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYAACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU5</accession>
    <name>Q8TUU5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1658"/>
    <dbReference type="EnsemblBacteria" id="AAM02871">
        <property type="protein sequence ID" value="AAM02871"/>
        <property type="gene ID" value="MK1658"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1658"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1822"/>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="OMA" id="IGKKLMW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="PTHR40734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="24279" checksum="BFD6DF56E5F9F475" modified="2002-06-01" version="1">MVEILYKQPKGKRAYEEYALILDFLPYGRAEEPPHKRQPVAQAIGKDFFVLLELAPKEGAFLELHEEVYVGRGKRDKIHHVNRRLRYDELTATAREELPHAVEKIIEDNEEDFVRFFNEARPITPRLHQLELLPGIGRKTLERILEEREKEPFESLDDIKERVKGLRMHPRDMIKERVLAELKGETRDKYRLFVPEFRGGRKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV6</accession>
    <name>ENO_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">MK1647</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUV6"/>
    <dbReference type="SMR" id="Q8TUV6"/>
    <dbReference type="STRING" id="190192.MK1647"/>
    <dbReference type="EnsemblBacteria" id="AAM02860">
        <property type="protein sequence ID" value="AAM02860"/>
        <property type="gene ID" value="MK1647"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1647"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1810"/>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_2"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000134025" description="Enolase">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="365"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="427" mass="46163" checksum="C7D9738D77BFC8C4" modified="2002-06-01" version="1">MPRIVSVKAREVLDSRGEPTVEVEVELEDGTVGRAMVPSGASTGTYEALELRDGDDRYGGKGVRRAVRNVEEIIAPEIEGLDATAQPDIDRTMIELDGTENKSHLGANAILGVSLAVARAAAKSLGIPLYRYLGGPTARRLPVPFMNVINGGEHAGNELDFQEHMIVPHGFESFSEALRAGVETYHVLGELLEEEYGPIATNVGDEGGYAPPMKDTVEPLDVLVEAIEEAGYAPGKEIALALDAAASEFYDEDSGTYRAYGQKYTRDELIDVYKDLVSQYPIVSIEDPLHEEDFRGFAKITEELGDKVQIVGDDLFVTNPDRLRKGIEMGAANALLLKVNQIGTLTEAVEAGELALQHGYGVMVSHRSGDTEDPFIADLAVALGCGQIKTGAPARSSRTAKYNRLLRIEEDLAGAAEFGPRNDFFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY8</accession>
    <name>Q8TUY8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="4">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">ftsY</name>
        <name evidence="7" type="ordered locus">MK1613</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1613"/>
    <dbReference type="EnsemblBacteria" id="AAM02826">
        <property type="protein sequence ID" value="AAM02826"/>
        <property type="gene ID" value="MK1613"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1613"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1776"/>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="6">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="157"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="239"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="297"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="349" mass="38297" checksum="BEF36508C3AD302B" modified="2002-06-01" version="1">MPSSLFGKMKSVLSKVKEKVAKRVEERAEEAEEAKVKEVETMPEDLKKPTLKERLKRVVKREVTITEADIEDILDELELELISNDVAVEVAESIREELKKELVGRRVKGKSEIPKVVEEGFREALLSVLEPKKEVDLMETVEKARQDGRPAIIMFVGVNGSGKTTTIAKVAKLLKDHGYSVVIAAADTFRAAAIEQLEEHAERLGVTLIKGERGDDPTAVAFNAVQHAEAKGKDVVLVDTAGRAYTDVNLMEELKKMKRVLEPDLVVFVGDALAGNDAIEQAKTFHEYVGIDCAILTKVDADAKGGAVLSISKVTGAPILYLGVGQDYDDLKAFSPEWFVERVIGGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV01</accession>
    <name>Q8TV01_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="6">IMP dehydrogenase</shortName>
            <shortName evidence="6">IMPD</shortName>
            <shortName evidence="6">IMPDH</shortName>
            <ecNumber evidence="6 12">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 14" type="primary">guaB</name>
        <name evidence="14" type="ordered locus">MK1600</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 12">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="6">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 12">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 11">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="6 12"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1600"/>
    <dbReference type="EnsemblBacteria" id="AAM02813">
        <property type="protein sequence ID" value="AAM02813"/>
        <property type="gene ID" value="MK1600"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1600"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1762"/>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_2"/>
    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 12" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8 12" id="KW-0520">NAD</keyword>
    <keyword evidence="4 6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6 9" id="KW-0630">Potassium</keyword>
    <keyword evidence="6 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="100"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="13">
        <location>
            <begin position="163"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6">
        <location>
            <begin position="344"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6">
        <location>
            <begin position="367"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="6">
        <location>
            <begin position="391"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="6 7">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 8">
        <location>
            <begin position="304"/>
            <end position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM02813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="502" mass="54340" checksum="A1A4E4963CC52279" modified="2002-06-01" version="1">MVGRYTRKLEDAELALTFDDVLLLPERSSVEPADVDVSTRVTVNYRINIPILSAAMDTVTEAEMAIAMARHGGLGVIHRNMTVEEQVKEVRRVKEARDVVQRDVVTISPDESVKRAVELMEKHDVGGLPVVDEEGKVVGIITRRDVGLLSEEEIGELDVKSVMTEEPVVIEEGEDLEERALRVMREEKIERVPVVDDEGRLLGIVTAKDVTELREETEAATDEERRYLAAAAVGPKDPDRAIALDEAGADILVVDCAHAHTETVINFVKEIKREVDADIIAGNIATAEAAEDLIAAGADALKVGIGPGSICTTRIVAGVGVPQITAVAWVADVAEEHDIPVIADGGIRYSGDIAKAIAAGADAVMLGNLLAGTDEAPGRVIRLRGRLYKQYRGMGSLGAMMKGESADRYFKQPEQGGRHVAQTKFVPEGVEGVVPYKGPVSEVLYTLVGGLRSSMGYVGAKNIEEMKKKARFVRITRAGYEESHPHDIAITDEAPNYPISNQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-28" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV04</accession>
    <name>PTH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="ordered locus">MK1597</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV04"/>
    <dbReference type="SMR" id="Q8TV04"/>
    <dbReference type="STRING" id="190192.MK1597"/>
    <dbReference type="EnsemblBacteria" id="AAM02810">
        <property type="protein sequence ID" value="AAM02810"/>
        <property type="gene ID" value="MK1597"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1597"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1758"/>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="OMA" id="GHAAVEC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PTHR12649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="SSF102462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120293" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <sequence length="116" mass="12871" checksum="D9556F87E16102CF" modified="2002-06-01" version="1">MYKQVIVVRGDLKLSRGKLAAQVAHASLGAFLRAKESGAPVEEWLREGQKKVVLKCKDKEELLELHELAKRRGLPSFLVRDAGLTELEPGTVTCLGIGPEREEEIDRVTGDLPLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV39</accession>
    <name>Q8TV39_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trxB</name>
        <name evidence="2" type="ordered locus">MK1561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAM02774">
        <property type="protein sequence ID" value="AAM02774"/>
        <property type="gene ID" value="MK1561"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1561"/>
    <dbReference type="HOGENOM" id="CLU_031864_5_6_2"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="299" mass="31810" checksum="6D7E4CD9EB7D7E29" modified="2002-06-01" version="1">MAKPYDVIVIGAGPAGLSAAIHAARAGAEVPVVAQELGGQLTTAPEVGNYPGFPPAPGYELVDRMVEHAETVGVELNVKYSNRVEGIELTDGTFRVLDEECRAVVIATGARPKRLGVPGERELEGRGVSYCAICDGPAFQNRIVAIVGSGTHAANTALFLSEIAERVYVITPDGKLESPDRALIERVLSCRNVEVVHDEVRRIVGDERVEGVELSDGDILPCEGVFIAAGKVPNSEPFRDLVETDDRGYIVVDSEMRTSLEGVYSAGDVTTIPHRNVPSAVYQGSVAGINAAEYALKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV43</accession>
    <name>Q8TV43_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OsmC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02770">
        <property type="protein sequence ID" value="AAM02770"/>
        <property type="gene ID" value="MK1557"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1557"/>
    <dbReference type="HOGENOM" id="CLU_1891441_0_0_2"/>
    <dbReference type="OMA" id="EMAPKMM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="134" mass="14526" checksum="308EF747DCCAFCA8" modified="2002-06-01" version="1">MADVIAEFLGDGECTVEFEEEKMELVSGKVAEPGHVTPYHLFLAGILACVTMNAGHALEKAGIDAEVTAEVTGEKDWDRLAVTEIEITIKVKLKDDTDPDEARKIAEKGADRCILSRTLGPAIVSKEVVVERES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV65</accession>
    <name>Q8TV65_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted enzyme of the squalene cyclase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1533"/>
    <dbReference type="EnsemblBacteria" id="AAM02746">
        <property type="protein sequence ID" value="AAM02746"/>
        <property type="gene ID" value="MK1533"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1533"/>
    <dbReference type="HOGENOM" id="CLU_488033_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016866">
        <property type="term" value="F:intramolecular transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="PTHR11764"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SQHop_cyclase_C" evidence="2">
        <location>
            <begin position="152"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="SQHop_cyclase_C" evidence="2">
        <location>
            <begin position="248"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="500"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="558" mass="62582" checksum="1518598699EF4BA9" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV69</accession>
    <name>Q8TV69_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwdA</name>
        <name evidence="2" type="ordered locus">MK1529</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1529"/>
    <dbReference type="EnsemblBacteria" id="AAM02742">
        <property type="protein sequence ID" value="AAM02742"/>
        <property type="gene ID" value="MK1529"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1529"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1691"/>
    <dbReference type="HOGENOM" id="CLU_035587_0_0_2"/>
    <dbReference type="OMA" id="GEKMDIC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01304">
        <property type="entry name" value="FMDH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="45"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="581" mass="65357" checksum="4AC93F66D563D358" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV79</accession>
    <name>Q8TV79_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1514"/>
    <dbReference type="EnsemblBacteria" id="AAM02727">
        <property type="protein sequence ID" value="AAM02727"/>
        <property type="gene ID" value="MK1514"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1514"/>
    <dbReference type="HOGENOM" id="CLU_2476024_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9768" checksum="F14000085ED17407" modified="2002-06-01" version="1">MRILRDLIGKPVIDSSAKHIGEVLDVEFDEESGEVTTLIVGHSKRPGVLRKIKWLGGEEKAVRIPYSNVVAIEDMVLVEGRWVSRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV80</accession>
    <name>Q8TV80_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02726">
        <property type="protein sequence ID" value="AAM02726"/>
        <property type="gene ID" value="MK1513"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1513"/>
    <dbReference type="HOGENOM" id="CLU_2067844_0_0_2"/>
    <dbReference type="OMA" id="TWFYIAV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="119" mass="13367" checksum="241CA0CF2C1AC3B1" modified="2002-06-01" version="1">MVLSIPDWLGAALLLSLSDELHTGLVWGALRSQYLLLGAYLRVRHSALFVWLVHETLEFLFHTVILSLILPVPMAVTAASVHFGIDLFHEALLGTVKGRVTHRLMHLSLESAILYWLWG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV83</accession>
    <name>HIS5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisH</fullName>
            <shortName evidence="1">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisH</name>
        <name type="ordered locus">MK1510</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV83"/>
    <dbReference type="SMR" id="Q8TV83"/>
    <dbReference type="STRING" id="190192.MK1510"/>
    <dbReference type="EnsemblBacteria" id="AAM02723">
        <property type="protein sequence ID" value="AAM02723"/>
        <property type="gene ID" value="MK1510"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1510"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1668"/>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_2"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152459" description="Imidazole glycerol phosphate synthase subunit HisH">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <sequence length="200" mass="22013" checksum="E46FEAD5E28EA035" modified="2002-06-01" version="1">MKVAIVEYGVGNLNSVYRAVKHLGHEPVVTDDPSELHDADAVILPGVGNFRAAAEKLEETGLCDEIRELLGSVPLLGICLGMQLLMESSEESPESRGLGVFRGTCVALPNDVKTPHMGWNTVEFRTEEFREFDGEMFYFVHSYRVAPEDDVVLGETEYGERFPSVIGDRGRLIYGMQFHPEKSGPVGLKLLGEVLTGASR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV84</accession>
    <name>GATD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="1">Glu-ADT subunit D</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatD</name>
        <name type="ordered locus">MK1509</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV84"/>
    <dbReference type="SMR" id="Q8TV84"/>
    <dbReference type="STRING" id="190192.MK1509"/>
    <dbReference type="EnsemblBacteria" id="AAM02722">
        <property type="protein sequence ID" value="AAM02722"/>
        <property type="gene ID" value="MK1509"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1509"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1667"/>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="OMA" id="RKNHTSR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="SSF141300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140053" description="Glutamyl-tRNA(Gln) amidotransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase" evidence="2">
        <location>
            <begin position="97"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01068"/>
        </source>
    </evidence>
    <sequence length="458" mass="50119" checksum="93F0448B0FB79EE6" modified="2002-06-01" version="1">MRVLSEYKGKAGELLREHGLSVGDRVRIVRDDGVVVEGIIMPRSELGDDEHIVVKMDNGYNVGVRVDRIEKLEAPGEGHEPSFKPMEGEIEYDPKLPNVSVMSTGGTIACRVDYETGAVKPAFTAEELVGAVPELLDVINIVDARAVLDLLSENMEPKHWMKIAEEVVDALSDPDVEGVVIGHGTDTMAFTAAALSFVIEGLNGPVVLVGAQRSSDRPSSDAASNLIAACAFAGDGEVGEVTVCMHGWTSDEVCLVHRGVRVRKMHTSRRDAFRSVESIPIAKVDVKDLRNPKIEFLRSDYRRPEDGEPEISGGFEEKVALVKFAPGMDPEVLDFYVDRGYRGIVLEGTGLGHVSEQWLESIERAVDDGIAVVMTSQCLYGRVNMNVYRTGRLLRAVGVIPGEDMLPEVAYVKLMYVLDRTDDIKEVERLMRTNIAGEIEGGRVLGGFEPADGPHHRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV89</accession>
    <name>NCPP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="1">ITPase/XTPase</shortName>
            <ecNumber evidence="1">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1503</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + XTP = H(+) + phosphate + XDP</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + ITP = H(+) + IDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YjjX NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV89"/>
    <dbReference type="SMR" id="Q8TV89"/>
    <dbReference type="STRING" id="190192.MK1503"/>
    <dbReference type="EnsemblBacteria" id="AAM02716">
        <property type="protein sequence ID" value="AAM02716"/>
        <property type="gene ID" value="MK1503"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1503"/>
    <dbReference type="HOGENOM" id="CLU_087417_0_1_2"/>
    <dbReference type="OMA" id="ADYWVGI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00258">
        <property type="entry name" value="TIGR00258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156361" description="Probable inosine/xanthosine triphosphatase">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <sequence length="170" mass="18316" checksum="13FB9E64A3238F5B" modified="2002-06-01" version="1">MKVRVGTTNPVKVRATERAFLRTFPDREITVEAVSVDPGVPPQPVGMEEVHQGAKNRAREAWNRGSYSVGLEAGLIRVGDVYVDLHVAVVRDPKGRETVGTSPGFQLPPDVTEEALSGEEVGEVFSELVGVREIGKRSGAIGVLSNGKVLREDLCELAILMALIGLETSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB1</accession>
    <name>PYRI_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrI</name>
        <name type="ordered locus">MK1481</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB1"/>
    <dbReference type="SMR" id="Q8TVB1"/>
    <dbReference type="STRING" id="190192.MK1481"/>
    <dbReference type="PRIDE" id="Q8TVB1"/>
    <dbReference type="EnsemblBacteria" id="AAM02694">
        <property type="protein sequence ID" value="AAM02694"/>
        <property type="gene ID" value="MK1481"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1481"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1638"/>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="OMA" id="CPNRNCI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="PTHR35805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="SSF54893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="SSF57825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000142332" description="Aspartate carbamoyltransferase regulatory chain">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <sequence length="156" mass="17786" checksum="565765010A1BDB7B" modified="2002-06-01" version="1">MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIEGKILSEEEANKVALVAPNATVNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREPVDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC2</accession>
    <name>Q8TVC2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted HD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02683">
        <property type="protein sequence ID" value="AAM02683"/>
        <property type="gene ID" value="MK1470"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1470"/>
    <dbReference type="HOGENOM" id="CLU_036524_3_1_2"/>
    <dbReference type="OMA" id="GHDWFHI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="29"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="200" mass="22988" checksum="4852B82867B41B1C" modified="2002-06-01" version="1">MKVNPKFPWEREVIEFVRTEMSEVSPSHDFEHVKRVVGLCEFIRRKEGGDPEILRAAAWLHDIGRPAEERSGEDHAEVSAEIAEDLLPRVGFPSDKLGEVTHAIRAHRFSTGPEPQTLEAKILQDADNLDALGAMGIARCFCAVGERGTSLESGVEHFHEKLLRLPELMHTETARRLAEERRRRMVLFLEWLEKEWRMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG0</accession>
    <name>Q8TVG0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1429</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02642">
        <property type="protein sequence ID" value="AAM02642"/>
        <property type="gene ID" value="MK1429"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1429"/>
    <dbReference type="HOGENOM" id="CLU_032214_0_0_2"/>
    <dbReference type="OMA" id="GPAAWQW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="49"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="212"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="509" mass="56877" checksum="A34C3BF3AB348833" modified="2002-06-01" version="1">MRGEETLAEVVLTMDRTLASNYRGGMFMGFSACVPKGIIPDWLYFSVFCPSVEYDEETGEVKEAPLGIRRIEAQLRREGYDVAVVHPDAVHKAIDEDTIAVGVSEIDPQGMGPATTTFTSFSGKPAYMKVCFEDLMERIRELKDRYGFGVFMGGPGAWQVAETFPRFGVDFLIMGEGEYVVGEVVRRIEEGDRGLEIVRGKPVAAEDIPTIVNPTTNGIVEVARGCGRGCKFCSPDMRELRSFPLSKILEDVDVNVRGGHEEILLHAEDVLRYKADGWRPNVEAVLELFSAVMNRPGVKRVSVSHVALSTVCQFDERLGEISEVAGVGELVPWMGAQVGVETGSPRLMAEHMPGKVAPYKVEEWPDVVEQAFGIMNDHGWVPCGTLILGLPGETEDDVMMTVELLDRLRDYKSFIVPLFFVPIGESRLSDHDFFTPEKLTEVHWEVILKCVDHDLKWLPELYEEYARANGHGPLVKLTIRALTWYGRRKIFKSALKWCPEKELVEAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL2</accession>
    <name>Q8TVL2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted nucleic-acid-binding protein containing a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1377"/>
    <dbReference type="EnsemblBacteria" id="AAM02590">
        <property type="protein sequence ID" value="AAM02590"/>
        <property type="gene ID" value="MK1377"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1377"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1532"/>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="OMA" id="IYYGTKF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF35_N" evidence="2">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="OB_aCoA_assoc" evidence="1">
        <location>
            <begin position="47"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="134" mass="15110" checksum="DAE61415DA8151E1" modified="2002-06-01" version="1">MGGVPRFWRSIDNRYRLVGTRCKNCGEVFFPPRVVCPNCRSDGEMEEVQLSGRGEVYTYSVVRVPPEGFEDKAPYVVAIVKLEEGPLVTAMIVDCEPGEIDVGTPVEAVFRRISEDGEDGVIYYSLEFRPVREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM1</accession>
    <name>Q8TVM1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase of the AAA+ class</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1368"/>
    <dbReference type="EnsemblBacteria" id="AAM02581">
        <property type="protein sequence ID" value="AAM02581"/>
        <property type="gene ID" value="MK1368"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1368"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1520"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_3_2"/>
    <dbReference type="OMA" id="FGEWAPK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="119"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="38512" checksum="3B18F8849E7C5BF2" modified="2002-06-01" version="1">MGYPVREPGMGKEVVVDSLEAFNAYAREQWLGEVVREGTILFDTGVVHSYAFKVVRVVPSGMGRITSSTRFVLRTRFEEDRMEIPNLTLDDVVGHEEAKRACSLLVEYLKNPEEFRDWAPKTVLFYGPTGTGKTHTARAVAGEAKVPLLHMNAAEILGKYVGEASERIRRAFTRARKAAPCVFFLDEIDALALDRRYQELRGDVVESVNALLTNLDRLKNEGEGVVFIAATNQPDILDPAVRNRFEYEIEFTLPNKREREELVRYYAKKLPMPLDVDPRYIAARTGGMSHREIKERVLKRALLEALREGAEKIERKHIQKVLNQERERRAVRIYHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM3</accession>
    <name>Q8TVM3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N5,N10-methylenetetrahydromethanopterin dehydrogenase (H2-forming)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmdII</name>
        <name evidence="4" type="ordered locus">MK1365</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1365"/>
    <dbReference type="EnsemblBacteria" id="AAM02578">
        <property type="protein sequence ID" value="AAM02578"/>
        <property type="gene ID" value="MK1365"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1365"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1517"/>
    <dbReference type="HOGENOM" id="CLU_069755_0_0_2"/>
    <dbReference type="OMA" id="IMTLQTM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010063">
        <property type="entry name" value="HMDII/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500166">
        <property type="entry name" value="HMDII_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01724">
        <property type="entry name" value="hmd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="54"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HMD" evidence="3">
        <location>
            <begin position="195"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="34471" checksum="65A14111BFF33F25" modified="2002-06-01" version="1">MATVAVLGFGNQRLYERIGAAEKLGGEPPFGGAAMAIEFAEAGHDVVLADPNLSEQDPEHVDRVADAGVELTEDDAQAVEGAEMVVLFTPFGATGGIIREIASHLEEGAVVCPTCTSSAFEIHESLYEAGLEVPEAVGVMPAHPAGIPGTENHRAYITARGTGNGTVLATEEQAELVEEVLSSTGKEVFELPHVELVSVVGDLSVVLLKRVIEALKEFCAVKALGAPQEMIDRQAMMTLATLAALIEAGGIGGLLETLDEEAIEASYSNMEPFVDGVEEPEGEPVERFVVLPGEATREAVIELVGERGWRTVRMRAWVDLYKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP4</accession>
    <name>Q8TVP4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK-34 family, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02557">
        <property type="protein sequence ID" value="AAM02557"/>
        <property type="gene ID" value="MK1344"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1344"/>
    <dbReference type="HOGENOM" id="CLU_1551834_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="290" mass="32754" checksum="706E6883976ADF91" modified="2002-06-01" version="1">MDVRSLYGLALDVTTLAVLELVKRYPASGEKLPEDRNSVELVLWEVKDLFRRFELGRLVDEEEFLEACFEVLRLPGVPVRVGGACEEAGLETLRNVFTDPDASVLFITSRGLDVFEDVMDGLGMDPKDVRAECRRLLVRDDGAVGEDAIESWVEAGFEVRVITDGESHLKVWYTDNVMAITGANMTNVSLERLRECREFVAPVVMDVKAPVALLEYEPLGFAAMVGANAHAVVLREFLTEPDRVYGGYLPECEGLRHLYRELRAGLEWYLREGCPEMLWWVEPEPRTPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR3</accession>
    <name>Q8TVR3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted regulator of Ras-like GTPase activity, member of the Roadblock/LC7/MglB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02538">
        <property type="protein sequence ID" value="AAM02538"/>
        <property type="gene ID" value="MK1325"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1325"/>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="OMA" id="MIRIEMK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="115" mass="12168" checksum="FD4361996DE4E3C4" modified="2002-06-01" version="1">MIEKVLADLNRVAGVNGSMVASSDGLVVAEAVPPEVDPEIVGAIATTVYGSGERVIEEMELGELKQMLVESTDGKVVIIRVDDDALLVLIADPDANLGLIRLKAREAAEEISKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS0</accession>
    <name>Q8TVS0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02531">
        <property type="protein sequence ID" value="AAM02531"/>
        <property type="gene ID" value="MK1318"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1318"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1453"/>
    <dbReference type="HOGENOM" id="CLU_619134_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="442" mass="50170" checksum="64B32520C06BE1F0" modified="2002-06-01" version="1">MTVLLVRNVGVNDVRVDGERVNEPYYVLKELVESGDLDGVSFPILERVLEELEPDRIVLFATDQDPGSTPRHLKAKDTLYVAEAMRELLDVEVEVRVIRENPSDHDAMLELYGRELPEVLDGAEEVYLDVTTGTPAQSLALALKGLEVEPDAELVYVSESTGTTRIRVGDELLRRIADELLEYGLNDPAARVLSHTGLDDLRTYAEARAAEDRFEFERARELYRGLPEDFLDVSDRLERVERALSDDPVERMAERTRYVLDRALTHLKRGEYGEYVLRTCVAQEMILWTTFSAVSGREPDPDEFVRHASREADWDPKYGNLGDLIRRDPVSTLARYLGDYLEDLTSLEVTDAGRAAAVNALASYYVVKGLRELRDDYAHRAIRITLEDVREALGRSRVDSEYWERKHGHGPDFKFPSEEDDPEAAVEALRECVDAIERAVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW02</accession>
    <name>Q8TW02_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase large subunit homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02449">
        <property type="protein sequence ID" value="AAM02449"/>
        <property type="gene ID" value="MK1236"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1236"/>
    <dbReference type="HOGENOM" id="CLU_570632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="161"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="319"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="478" mass="50756" checksum="550A9D113F125157" modified="2002-06-01" version="1">MTSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATCGPGVAVALAGLACALADRVPVLCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTLARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRGLLEGKRVAILVGRGCYRARCGELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLSGRTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLELYRVVKAALKAWIGPATVDSGQITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASARVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNGELGFTRQVMEEKEGRAAKMGLDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-29" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW19</accession>
    <name>RL24_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24</name>
        <name type="synonym">rplX</name>
        <name type="ordered locus">MK1219</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW19"/>
    <dbReference type="SMR" id="Q8TW19"/>
    <dbReference type="STRING" id="190192.MK1219"/>
    <dbReference type="EnsemblBacteria" id="AAM02432">
        <property type="protein sequence ID" value="AAM02432"/>
        <property type="gene ID" value="MK1219"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1219"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1322"/>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="OMA" id="VRIMRGD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_L24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_L26/L24P_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="PTHR11143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130771" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="149" mass="17581" checksum="32F1EEEC6137076E" modified="2002-06-01" version="1">MRWTKSSQPRKQRKAFFNAPLHKRQKLMSATLHPELRKKFNRRSLPVRRGDMVRIMRGDFKGHEGEVVEVDLKRLRIYVEGATIERANGEKVYYPIHPSNVMIIEPNLDDPMRRKIIERSGGTPEVEAVPEKSEEEKEEKEKEEEKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW47</accession>
    <name>Q8TW47_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase subunit of an ABC-type transport system, contains a duplicated ATPase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1189</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1189"/>
    <dbReference type="EnsemblBacteria" id="AAM02402">
        <property type="protein sequence ID" value="AAM02402"/>
        <property type="gene ID" value="MK1189"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1189"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1290"/>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="DPMTRLD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="267"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="304"/>
            <end position="311"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAM02402.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="568" mass="63583" checksum="298EEF28ED3BFE9D" modified="2002-06-01" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
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        </source>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="7"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAM02399.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="77" mass="9098" checksum="05FE726E9B171546" modified="2002-06-01" version="1">MSEAELVRELIEELRALRHEIYMLRMELERLRGEVEEVESEDDIPDLLRRAERAAEMAVGDIDLELESDEEGRWSLL</sequence>
</entry>
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    <accession>Q8TW52</accession>
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        </submittedName>
    </protein>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
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        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
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            <end position="118"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="131" mass="12389" checksum="46CDE8452DADE2D9" modified="2002-06-01" version="1">MLGTLGFRAAVMAAILAYILTVPSHLGGVGKSVEDIAKEAIGGGDKGSAGEKDEEVSEKGGEGKGEKSGGERRPEEGSSGSTGGGSSGGNGTYAGSSSSGGGTVVTTGSSSGSSGGFVCTGNCAACPNPCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW54</accession>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
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                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            <dbReference type="EMBL" id="AAM02395.1"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="238" mass="25208" checksum="EFD1B9273899BAC2" modified="2002-06-01" version="1">MLQALAFVLLLTIAPAAVHAGQVTVNVALDDMVSNGAELGTYDTLVSKLTLDVAVTDVGTGTTHTAEFKLSEVPKSVTFEGIRGPVTVSVTLKYDGKTALAATLFPDWPCSSATVDTPDGMLQARVGCVSQYVADTEFSTECVVAAELYPGLHSTEIPEDARYVLAKFNVKVKAEGLAHGGSRARIHLDDVLDTAMDLAELVLKYMPPILIVAPVLVFIGPTAVEMFAEFLKAILVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-02-26" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB0</accession>
    <name>Q8TWB0_METKA</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
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            <dbReference type="PubMed" id="11930014"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
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            <dbReference type="EMBL" id="AAM02339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="276" mass="30812" checksum="835D7B1D604F9685" modified="2002-06-01" version="1">MIVVGAGTFGCRVAPYVASAFEASSVVLVDTPDGIQSGEELAKKLGLQTSRVEVAYGSPSGAHRDYETAMKDAEKSEALKQLSEKFVEVVKLEYRADRVPVLVWGLGGAIGAATAIRLGEEVPYALHVTVLPDYRSEVEPVRRLLFNAKKQLQSFMGRLKRHPVIIGDFAGVPADKVGEHLVCILSRRHEIVDKRRVQTANYVRDGDDEIRMAFLVDVNRYGLQRVQLDRIISQIQEMLEWAEDFVECDLAGRIRAARLEESLRTGIWDNDWKEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB8</accession>
    <name>Q8TWB8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02330">
        <property type="protein sequence ID" value="AAM02330"/>
        <property type="gene ID" value="MK1117"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1117"/>
    <dbReference type="HOGENOM" id="CLU_2476023_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9547" checksum="4C384AABAB3D2FE5" modified="2002-06-01" version="1">MAGLPPWMAGALYGLIEAIGWDELRPEALHDLMWRLTFHTVTASAPTIPAVLYHALVNGSPYKWTTTAGLLVDLLTYSLSAFFGLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL7</accession>
    <name>Q8TWL7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Archaeal/vacuolar-type H+-ATPase subunit F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ntpF</name>
        <name evidence="4" type="ordered locus">MK1016</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1016"/>
    <dbReference type="EnsemblBacteria" id="AAM02229">
        <property type="protein sequence ID" value="AAM02229"/>
        <property type="gene ID" value="MK1016"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1016"/>
    <dbReference type="HOGENOM" id="CLU_135754_2_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="SSF159468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="112" mass="12275" checksum="0C1B3DF587DEF5FC" modified="2002-06-01" version="1">MYVVAAVVNGSVASGFRLVGVRTIDADREDPKEAVKKLASDPEVGVIILTEDVAEKVKEEVEEIQREKVSRGEATPIFVTVPGPEGPTEEFEIEEIVKKAVGVEIDLERIER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-09-21" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM5</accession>
    <name>SECF_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secF</name>
        <name type="ordered locus">MK1008</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWM5"/>
    <dbReference type="SMR" id="Q8TWM5"/>
    <dbReference type="STRING" id="190192.MK1008"/>
    <dbReference type="EnsemblBacteria" id="AAM02221">
        <property type="protein sequence ID" value="AAM02221"/>
        <property type="gene ID" value="MK1008"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1008"/>
    <dbReference type="HOGENOM" id="CLU_060478_0_0_2"/>
    <dbReference type="OMA" id="TWLMNLG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000412710" description="Protein-export membrane protein SecF">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <sequence length="274" mass="29148" checksum="0038F19527AEF361" modified="2002-06-01" version="1">MGLFEKYVSNLNRLLILTMVFAVICAGSTLALGVKKGIDLKGGTMVILKTEKDPDTVTSEASRILGVSDVEAIRSSQGDVIVQVPKYLSADDVNKLARAVGGEVESVQTIGPALGRVFWESVKVAVPLALVAVSIVVFAIFRKPLLSAAVLGALALDLVDALGLMALTGVPLTLASFAGLLMIIGYAVDSNILLSMYTVKRRRVRRVDRAIADSFKTGITMVATTTAAACALFLLSMSEAMFEIAAVVIFGLIADVLNTWIFNAWVIREKIAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN6</accession>
    <name>Q8TWN6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaea-specific enzyme of the ATP-grasp superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0996"/>
    <dbReference type="EnsemblBacteria" id="AAM02209">
        <property type="protein sequence ID" value="AAM02209"/>
        <property type="gene ID" value="MK0996"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0996"/>
    <dbReference type="HOGENOM" id="CLU_075000_0_0_2"/>
    <dbReference type="OMA" id="MPVRIYE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="26506" checksum="81033482A89B2C8E" modified="2002-06-01" version="1">MEVEFIRLADEIPECEFLIEGYPGIGMVGVIAIRHVVTECGAEPAFVMKIEDMPTAAIVHEGRVYPSAGVFVLDDLSFFYAEQADMRPGVVQNISESVAELSREIGVECIVCLAGIRAPDLEDEPSLYYAATSDKAAELFEGIAEPLKGGTITGVSGPLLLEGSLRGLDAVCLLVETPGTYPDPKAASRVVEALNEALGLSVDVSKLEEEAERIAKAVEETVQRLREQQRMEEEEAEPTERMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP3</accession>
    <name>Q8TWP3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0989"/>
    <dbReference type="EnsemblBacteria" id="AAM02202">
        <property type="protein sequence ID" value="AAM02202"/>
        <property type="gene ID" value="MK0989"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0989"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1036"/>
    <dbReference type="HOGENOM" id="CLU_048071_0_0_2"/>
    <dbReference type="OMA" id="CIFCSVD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040088">
        <property type="entry name" value="MJ0103-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF35">
        <property type="entry name" value="PTHR11228:SF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01110">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="107"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="5">
        <location>
            <begin position="357"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="419" mass="47680" checksum="DD17C11762D03C48" modified="2002-06-01" version="1">MKVERHSGYLRFRLYGLIEFEVDPEEFRRMVTAVISKRERRTVPVTLQGTRVAAPVPREVLVGVLTDLISRLAGESGKNAYTGRRVYYITETTGIPLIGHTAFGLIDRGTNVIQVRPLSGCNLCCIYCSVDEGPISRTRSRDFMVDPDYLMEWFDRVAEFKGQGLEAHLDGQGEPTLYPFLPDVVQALKEHPHVDIVSIQTNAVPLSEDLVDELVEAGIDRFNVSVNSLDPKKARAMAGRKDYDVEHVKRVVEYIAQETEADVLVAPLWLPGYNDDDIVEIIGWAAKIGAGKRWPPLGIQNYLEYRFGRRPKFLRRVIPMKEFYRWLRELESRTEVRPLVLKPEHFGTEPRKSLPKPFRRGDVIRAEIVLEGRLRGEMIARAADRVIAIPDSAKILNVGDRVRVKVTRDKHNIFVGILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ3</accession>
    <name>Q8TWQ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-15 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02192">
        <property type="protein sequence ID" value="AAM02192"/>
        <property type="gene ID" value="MK0979"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0979"/>
    <dbReference type="HOGENOM" id="CLU_896052_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="310" mass="34032" checksum="E14BE42EEE17546F" modified="2002-06-01" version="1">MRWNLTVGVTALALILLVTALAPSYIPAACLLLEGGSRPKLVAFLGLLTAFSLGSAVVRKRPDPLITSLLLLVLPFTSNLLHCVAAKSIGVPPGSEFFVFNGRVLEGDSPLHTHIGKAALTWALERVCRISLYIHCGMSLVETYPTYVLAAEFVVVSLSVLLGVFNTRDPLSCLASSLLVLSSIDGGAFSLPYVHGSWLLALRYVREPELMIILLWFAALSPYCKLVLGTLLTHAYYGERWDSVRLQVLAGPLDGPLKSLGGVRCDGYYVLPVRSCAEWKEFLRNLEDSLRRSKVRWVAFCTFPNMAMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ4</accession>
    <name>Q8TWQ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichol kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sec59</name>
        <name evidence="2" type="ordered locus">MK0978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0978"/>
    <dbReference type="EnsemblBacteria" id="AAM02191">
        <property type="protein sequence ID" value="AAM02191"/>
        <property type="gene ID" value="MK0978"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0978"/>
    <dbReference type="HOGENOM" id="CLU_031477_4_2_2"/>
    <dbReference type="OMA" id="CKREKED"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="PTHR31303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="193" mass="20285" checksum="6F404ACF3F3B1912" modified="2002-06-01" version="1">MTSKELGRQLIHATFGLLFLLPLHMLGVYEFALLLLSGLIAGVSVSFALRRGLHVPIAKELVDAFERPDEMHIPGQGTLHFVTGLLLATIICPYTKVLDVTIIVLSVGDSASTIAGKAIGRIPIPYSSRKTVEGSLVGFTAAALASLAWTGDVVVSALAAGVGMLVESLPTPNDNVTIPVAVSVALGFWWGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV3</accession>
    <name>Q8TWV3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized Fe-S protein PflX, homolog of pyruvate formate lyase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pflX</name>
        <name evidence="6" type="ordered locus">MK0928</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02141">
        <property type="protein sequence ID" value="AAM02141"/>
        <property type="gene ID" value="MK0928"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0928"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.970"/>
    <dbReference type="HOGENOM" id="CLU_062674_0_1_2"/>
    <dbReference type="OMA" id="GGEPTPN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01099">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="119"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="343" mass="40443" checksum="97E4961F8BFEF3A5" modified="2002-06-01" version="1">MRPVLEPLAEYHSIDDDELAARFMVARTLPVETTEGELEDLWKEHEEAMEEFRARWEERPAPGDIEETSPNLLDLKLEIARKILRECTFCERRCRVDRTREDGFCRVPIKPRISSEFLHMGEERVLVPSHTVFFCGCTFRCVYCQNWDIAFRPTNGVYVKPESLARVIRHRRSQGAKNVNWVGGDPTPNIHYILEVLRRLDVNVPQVWNSNMYLTEEAMRLLDGVIDLYLTDFKYGNDECAERYSNAPNYWEIVTRNHREADRQCGLIVRHLVLPGNVECCTFPILEWIAEELGTDTPLNVMPQYRPEHRAYEYPEINRRPSADELEEAWEKARELGFRYYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV8</accession>
    <name>Q8TWV8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0923</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02136">
        <property type="protein sequence ID" value="AAM02136"/>
        <property type="gene ID" value="MK0923"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0923"/>
    <dbReference type="HOGENOM" id="CLU_2911528_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="61" mass="7196" checksum="3385B5E6DD2D39BE" modified="2002-06-01" version="1">MIGLFRGKRREIDYSIDRRELIEVVCEIRDVRNSFAHVREDVGRPDEKAQNLVKKVKEIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW2</accession>
    <name>Q8TWW2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0919</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0919"/>
    <dbReference type="EnsemblBacteria" id="AAM02132">
        <property type="protein sequence ID" value="AAM02132"/>
        <property type="gene ID" value="MK0919"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0919"/>
    <dbReference type="HOGENOM" id="CLU_083873_6_1_2"/>
    <dbReference type="OMA" id="INFIGIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="22"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="151" mass="17174" checksum="6B431E278EEF47DD" modified="2002-06-01" version="1">MKILFRILERTPGVDGPERFLKFSLVGLSGVFVNLGLLWFLTEVVGIYYVVSNIIAVEVSIISNFILNDLWTWRDRRDPGLLNFLKRLAAFNIICAGGLVINTAVLWTLTELLHIYYIASALFGIAAATLWNYWMNNRVTWGVLLERAHEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX5</accession>
    <name>Q8TWX5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted protease of the collagenase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2015" name="Acta Crystallogr. D" volume="71" first="2505" last="2512">
            <title>The first crystal structure of the peptidase domain of the U32 peptidase family.</title>
            <authorList>
                <person name="Schacherl M."/>
                <person name="Montada A.A.M."/>
                <person name="Brunstein E."/>
                <person name="Baumann U."/>
            </authorList>
            <dbReference type="PubMed" id="26627657"/>
            <dbReference type="DOI" id="10.1107/S1399004715019549"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="5D88">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.66 A"/>
        <property type="chains" value="A=1-234"/>
    </dbReference>
    <dbReference type="PDBsum" id="5D88"/>
    <dbReference type="SMR" id="Q8TWX5"/>
    <dbReference type="EnsemblBacteria" id="AAM02119">
        <property type="protein sequence ID" value="AAM02119"/>
        <property type="gene ID" value="MK0906"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0906"/>
    <dbReference type="HOGENOM" id="CLU_1182885_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5D88"/>
        </source>
    </evidence>
    <sequence length="234" mass="26240" checksum="EB8B188CDBD642FD" modified="2002-06-01" version="1">MTRRWYLCCSRHHLDTVPEDSDGIVVPVTEHGVATLLPRYPETYEVEDIVDVAKDRGLSVQALMDFTCAGCEHLSPDGYPSLRSTLDYLASDLEVDGVVVADPYLVEVLATEYDLTVVVSHTAAVDTPEKAWHFERLGADVITVDPALNSNEEEVSAIRERVSVELRTAVGAITFRDPVAFFERNLFSHATAEGIEVDPYRNNPYEPMRERVVVWEVREELFDEVFILASGEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX02</accession>
    <name>Q8TX02_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02092">
        <property type="protein sequence ID" value="AAM02092"/>
        <property type="gene ID" value="MK0879"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0879"/>
    <dbReference type="HOGENOM" id="CLU_1048117_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="15"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="265" mass="30057" checksum="0F99FC079700B17C" modified="2002-06-01" version="1">MGERTVAHYGPYVVKEIRTNQDNLDAIRSLLKSAERRGPGNVGEVVPLDVFDTDRGLASDRTRLLVALREDEIVACVAADPRERIGSGPNPHNVFGLVVSPEVRARYQLGHVMIAAALKTLREEGLKVARTTPIRRALPFFTGIRADPTHVYRELEVRLRWYRRTGSEEYLELTRVPREVREELELLRIRTVNARRDPTPFHRLDPEKPVHVFRSGVRSFTLIASLGDGDHGRRQRDIAHVQRAREPSKGDSQDRGGSGGRRLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX08</accession>
    <name>Q8TX08_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannose-6-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cpsB_1</name>
        <name evidence="2" type="ordered locus">MK0873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02086">
        <property type="protein sequence ID" value="AAM02086"/>
        <property type="gene ID" value="MK0873"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0873"/>
    <dbReference type="HOGENOM" id="CLU_2257384_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="28"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="11453" checksum="17D03BB7B0728C53" modified="2002-06-01" version="1">MTVSAEGERGPCGRVRELYSNDKVSVAEVSVRGEGEEHYHRRTFEVYYVIDGRGKVVLNGRPVDVGPGDVVAIEPGTRHKVIGNLRMLVVCIPPFDPEDVYRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX15</accession>
    <name>ARGJ_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glutamate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">Ornithine acetyltransferase</fullName>
                <shortName evidence="1">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglutamate synthase</fullName>
                <shortName evidence="1">AGSase</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">argJ</name>
        <name type="ordered locus">MK0865</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="1"/>
    <dbReference type="EC" id="2.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX15"/>
    <dbReference type="SMR" id="Q8TX15"/>
    <dbReference type="STRING" id="190192.MK0865"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="EnsemblBacteria" id="AAM02078">
        <property type="protein sequence ID" value="AAM02078"/>
        <property type="gene ID" value="MK0865"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0865"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.907"/>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_2"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103045">
        <property type="term" value="F:methione N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000002275" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002276" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="1">
        <location>
            <begin position="173"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <sequence length="387" mass="41840" checksum="ED311EC1F47D56DD" modified="2002-06-01" version="1">MRAPEGFLLGGIKREGIGVGLIFSERRCAVAGTFTENTLRAAPVEHSEEVCDRGVARGVIVNSGHANAMTGEEGYQDVLRTAEAIAELMGAPEDEIVVCSTGVIGERPPVDKIVRYAREVWEDIGPTERHVREFSRAIMTTDTEEKIALYEGDGWSLLGIAKGAGMIHPNMSTMLAFLLTDVGAKPKELQMWLRDVVNDTFNMITVDGDESTNDSVVLLANGSSNLKVGSDVTITEFQRALEEVCTELAEKIVRDGEGATKLMIVCVHGASNEVEARRAARAIASSNLVKAALFGENPNWGRIGAAVGAARVDVDPDELRIAFRSSEGEIVTYEGGPVDFDEEKAKRVLSASEVEIVVDLGVGDASARAWGCDLTYEYVRINAEYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX24</accession>
    <name>Q8TX24_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted UDP-N-acetylglucosamine 2-epimerase of the MurG family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0854</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02067">
        <property type="protein sequence ID" value="AAM02067"/>
        <property type="gene ID" value="MK0854"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0854"/>
    <dbReference type="HOGENOM" id="CLU_039259_0_0_2"/>
    <dbReference type="OMA" id="IYNPIEM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="323" mass="35518" checksum="06AD0EEC1A1FD1B1" modified="2002-06-01" version="1">MKAFSLVEPSPAKNLLPVWERLLEEGWEVTVYGHGRGVEILKWEGIETKQLGKPRRRQGLLGYVGFSVDLIRTVRALASERASVVLSSGNTGDARKSLIASRMLGIPSVHLEMDVYNPVEAVRWATRVLVPFSEKWAEVLKRRVGVETKVVSGAPLAQYLADRHLSGRIPGPVPEEYEGRVLVCLGGDITEEGARRLLSDLEDHDPVVVPFRVSPPEGFECVREFVDLPGVMMLADTVVFAGGFGVTIEACVVAKKAVKVSEVHPKHLSHWIAEESGVPVMYLSESSGAEDAVQMARKPRGEWLIRRGRSAVAEIVEEILEVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX36</accession>
    <name>RL22_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl22</name>
        <name type="synonym">rplV</name>
        <name type="ordered locus">MK0841</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX36"/>
    <dbReference type="SMR" id="Q8TX36"/>
    <dbReference type="STRING" id="190192.MK0841"/>
    <dbReference type="EnsemblBacteria" id="AAM02054">
        <property type="protein sequence ID" value="AAM02054"/>
        <property type="gene ID" value="MK0841"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0841"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.884"/>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="OMA" id="ANAEYKG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_L22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_L22/L17_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="PTHR11593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125276" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="159" mass="18830" checksum="8870742F2C96D0E1" modified="2002-06-01" version="1">MPKTKHWHYSIPPEELDDERTAKAFIRELRISPKHAREICRAIRGMPLDRAKEFLRRVIRKEEAVPFRKHKKKVPHRRQIRPGWDAGRFPEKAAREILRVLEHAEANAEYKGLDTDRLYIKHIAAHKGRVIRGWIPRAFGRATPFNTPTTHIEVILEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX57</accession>
    <name>Q8TX57_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyl-tetrahydropterin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0819</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0819"/>
    <dbReference type="EnsemblBacteria" id="AAM02032">
        <property type="protein sequence ID" value="AAM02032"/>
        <property type="gene ID" value="MK0819"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0819"/>
    <dbReference type="HOGENOM" id="CLU_111016_3_0_2"/>
    <dbReference type="OMA" id="IGQGACY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="160" mass="18305" checksum="9C9C2E4BD007E576" modified="2002-06-01" version="1">MPRVYLDGRELGLRFSACHVIPGHGKCGRLHGHTYHVSVELLGERTEPHGFVYDFDELKSTVRELIKPLDHRVLLPTESELFEIEESGNEITVRLSDGKRYVFPREDVVLIPTRSLSAEDLAEYLADELERRLAGDNLKELRVRVDEGWGQGAEVVRRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX62</accession>
    <name>Q8TX62_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0814"/>
    <dbReference type="EnsemblBacteria" id="AAM02027">
        <property type="protein sequence ID" value="AAM02027"/>
        <property type="gene ID" value="MK0814"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0814"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.856"/>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="OMA" id="VRHTSCS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="143" mass="15734" checksum="4C8B28A1FBBEDD0B" modified="2002-06-01" version="1">MSPGVRALSVYQAELRIKSERRVQVIDVTDQVREKVRESGVEKGIAHVYSRHTTAAVVVNEPESGLLRDIVNKLEELVPQGAGYEHDRIDNNADAHLRALLLGSSVTIPVSDGDLVLGTWQSVLFVELDGPRSRRLLVTVVGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX99</accession>
    <name>IDI2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="synonym">lldD</name>
        <name type="ordered locus">MK0776</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01990" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01990.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX99"/>
    <dbReference type="SMR" id="Q8TX99"/>
    <dbReference type="STRING" id="190192.MK0776"/>
    <dbReference type="EnsemblBacteria" id="AAM01990">
        <property type="protein sequence ID" value="AAM01990"/>
        <property type="gene ID" value="MK0776"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0776"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.817"/>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="OMA" id="WDWGIPT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="FMN-dep_DH"/>
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    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134445" description="Isopentenyl-diphosphate delta-isomerase">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="4"/>
            <end position="5"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>FMN</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="267"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="288"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="365" mass="39272" checksum="B5C0541EBB49C355" modified="2003-06-16" version="2">MRERKWEHVLACIWEDVESEESPLFDCVKIVHRALPELDFDDVDMEIELFGKRLSFPLIIAGMTGGHPKTGEINRKLARVARELEIGIGVGSQRAGVKDPEVRWTFEVVREEYPDGLVLANIGLPQLRENGPDLALEVVDMVDADALAVHVNVLQEAVQLEGEADAAGFVDVLAEVCETVDVPVVLKETGAGVSAEDAKLVRDIVDGIDVGGAGGTNWAVVEAVRSKAHGEIPLGYAFSDWGVPTAASILEVRSVVGNDLAIIGTGGVRTGMDVAKVLALGADCAGMALPVLRKVLAEGVRGCVRFLKSIAREVKIAMLMAGCSSVEEMSSVPIVVYGKLREWLECRGVPLDLVCTGDRRTGWNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA5</accession>
    <name>Q8TXA5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAM01984">
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        <property type="gene ID" value="MK0770"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_048072_1_1_2"/>
    <dbReference type="OMA" id="WFGECAA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="331" mass="36904" checksum="3B27C993839FA067" modified="2002-06-01" version="1">MKRRAIPAMAAGVAVVLIMIWSFDPRKVLTVIARTEPRLFALAVCIQLVDLLLWALRWHLVLVRGGVKAPFRLVFAVNNVSMLVNNITPSARSGGEPLRVYLLARMTRYRARDIASSVVIDRVLDYFPLTLLLLLSAFLIAGSGGRGGILVILLGGVSFLTAALILSLWFLASERYVHRVARGVLRLLSRVSRRVRARRLWEELDEWVERFVKQLRELLQDRLTLIQGTLLSAAVWGCEILRTYVVFLSLGREVPLPVIVVSFTVSMFAGVLPLLPGGLGLVEISTASVYRLWGIDPGTSAAVALLDRLISYWMVNAIGVISLLRISRRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA6</accession>
    <name>Q8TXA6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoesterase</fullName>
            <ecNumber evidence="2">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0768</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0768"/>
    <dbReference type="EnsemblBacteria" id="AAM01982">
        <property type="protein sequence ID" value="AAM01982"/>
        <property type="gene ID" value="MK0768"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0768"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.808"/>
    <dbReference type="HOGENOM" id="CLU_063749_4_0_2"/>
    <dbReference type="OMA" id="ACGCATE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="165" mass="18153" checksum="E363F7572A78F3A7" modified="2002-06-01" version="1">MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGNNDGERDYLREKAEEVGFELVGEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGHTHEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKVEFVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC1</accession>
    <name>Q8TXC1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UDP-N-acetylmuramyl pentapeptide phosphotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mraY</name>
        <name evidence="7" type="ordered locus">MK0753</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0753"/>
    <dbReference type="EnsemblBacteria" id="AAM01967">
        <property type="protein sequence ID" value="AAM01967"/>
        <property type="gene ID" value="MK0753"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0753"/>
    <dbReference type="HOGENOM" id="CLU_023982_0_1_2"/>
    <dbReference type="OMA" id="HSFELHG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="37959" checksum="7D519CF2639D1952" modified="2002-06-01" version="1">MIIETFTLSTVTSAVTVGLLRKYMREANVVDRPIVTEHAHKSGMPIMGGLGILLGTASGPLLASIQALPPIASALPMMVLGIVDDLLGLQVEEFQKVVRNVSNRPIELGRLILQPGEEARVATEKAKRDLKRILEEDPDAIEIVGEVPIKKELSEREKLALQTGAALFVLPIVPATWLWIPKVGVWNLSYLFYPVAVFGIVGATNAVNLIDGMDGLAAGLLFLASLACSGVCLGQGQLPGAAFFASLAGASLGFLFHNRYPAKIFMGDTGSCFLGAAYAAGALYYKIEIPAMIALGVPVLSTLISLLHRAGVIRLAVEPLHHHIQYKYDLPEPVVVALHWTAGAVFAAVALWISVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-03-29" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK0</accession>
    <name>SPCS_METKA</name>
    <protein>
        <recommendedName>
            <fullName>O-phosphoseryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="2">2.9.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Selenocysteine synthase</fullName>
            <shortName>Sec synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Sep-tRNA:Sec-tRNA synthase</fullName>
            <shortName>SepSecS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">spcS</name>
        <name type="ordered locus">MK0672</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + O-phospho-L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:25041"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SepSecS family.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.2" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXK0"/>
    <dbReference type="SMR" id="Q8TXK0"/>
    <dbReference type="STRING" id="190192.MK0672"/>
    <dbReference type="EnsemblBacteria" id="AAM01887">
        <property type="protein sequence ID" value="AAM01887"/>
        <property type="gene ID" value="MK0672"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0672"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.710"/>
    <dbReference type="HOGENOM" id="CLU_022508_0_0_2"/>
    <dbReference type="OMA" id="HIINGAY"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098621">
        <property type="term" value="F:phosphoseryl-selenocysteinyl-tRNA selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019872">
        <property type="entry name" value="Sec-tRNA_Se_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944">
        <property type="entry name" value="PTHR12944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017689">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03531">
        <property type="entry name" value="selenium_SpcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0711">Selenium</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000219882" description="O-phosphoseryl-tRNA(Sec) selenium transferase">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetramerization" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate loop (P-loop)" evidence="3">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q6LZM9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q6P6M7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="431" mass="47589" checksum="4557FD9AF640CA03" modified="2002-06-01" version="1">MRGLIPDHMLERGRTVLDSYREPVERLLSERRMPEEGWPDDVIATFLWELSRMDTDKDPKAARIGEREARVASRLAEESVFGFCHGVGRSGTLVDPQPKAPGASIMYALTNRLVTDFLRRLGFRIEGAFVVPGATGLSIALCLSALGEGEEVIYPYAAHKSPIKAVRLAGFGMRVVDTEIEGDRIVVDPGDVEEALERSESPAAVLSTLTFFPPRSSDPLPEIAELCEEYGVPHVVNAAYGIQHEQYRDLLNRAIKRGRVDVVVSSTDKNLLTPVGGGIVYAPDEETLREVSRAYPGRASAAPVAHALISLLSLGMKGYRRLMRRQKECKALLDELLEDLEARRDDVRVLDVDNPIASAVAVEGHDPVDLAARLYVRRVTGPRGVRADDPFGTSRLRGYHSNYITINAAIGVREEDVKTAVERLERELEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK5</accession>
    <name>Q8TXK5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine synthase II (Cobalamin-independent)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">metE</name>
        <name evidence="2" type="ordered locus">MK0667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0667"/>
    <dbReference type="EnsemblBacteria" id="AAM01882">
        <property type="protein sequence ID" value="AAM01882"/>
        <property type="gene ID" value="MK0667"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0667"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.705"/>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="OMA" id="DPDCGMR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33441" checksum="8DC4516D167215D3" modified="2002-06-01" version="1">MRPVVTVVGSHPVGHEPTLKDRLLEKLRRRSAYAPAIHEAVRDQTEAGVELVSDGQVRGDMIEIFASHIPGMTVEDGPAVIGRVEPPRFSPLVLDYREATRVAGEVEVKAILTGPVTLCYSLEVRTDLYPSNDHSSLLKDVARALSAEARLLRREGASVLQVDEPILSAGVTSVKKVARYVNTVLKAFKGGTRVLHVCGDVTEVYLDLEENIDADVFDHEFAGHPENLEVVAEGDSPIGVGVVRSDTDRVESLDEVVGLLEKAREAMGDRIEFVDPDCGLRKLPREIAKKKLEVVRKARDRVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL0</accession>
    <name>Q8TXL0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mcrG</name>
        <name evidence="8" type="ordered locus">MK0654</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0654"/>
    <dbReference type="EnsemblBacteria" id="AAM01869">
        <property type="protein sequence ID" value="AAM01869"/>
        <property type="gene ID" value="MK0654"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0654"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.693"/>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="OMA" id="GHRNPGE"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="29920" checksum="D60F5441979E67ED" modified="2002-06-01" version="1">MAEKAQFYPGETDVAENRRKYMNPNYELKKLREIPDEDIVRLMGHREPGEEYPSVHPPLEEMEEPECPIRELVEPTEGAKAGDRIRYIQFTDSVYFAPIHPYIRARMYMWRYRGVDTGSLSGRQIIEVRERDLEKIAKELLETEIFDPARSGVRGATVHGHALRLDENGLMFDALRRYRLNEETGEVEYVKDQVGIELDEPIPVGAPADEDDLKERTTIYRIDGTPYREDEELLQVVQRIHELRTLAGYRPEEAEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL5</accession>
    <name>Q8TXL5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01862">
        <property type="protein sequence ID" value="AAM01862"/>
        <property type="gene ID" value="MK0647"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0647"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="232" mass="26505" checksum="EEDEFD43CCDB36D8" modified="2002-06-01" version="1">MAGLPTARPDATPVRAPERIRVPEPPRDPVEQARVLRVLLETVRRGVLPFLGASYRSVNGKVYGPYYEARWKPRKGERGRTIYLGKSENESVRFLEAWLWEVRRAAPRLAEHRKAKWFVARAVRRALAALLARFEGDPEEALELAGEVLRKVGGILTGMPFDPLRTYSRPRGGRKLLRMLLGRTPGEFRDVLLEMLSPWPPWYCLTLLERLHGRTAAREYHRARVPRPGSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP4</accession>
    <name>Q8TXP4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted ATP-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">lonB</name>
        <name evidence="4" type="ordered locus">MK0616</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0616"/>
    <dbReference type="EnsemblBacteria" id="AAM01831">
        <property type="protein sequence ID" value="AAM01831"/>
        <property type="gene ID" value="MK0616"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0616"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.653"/>
    <dbReference type="HOGENOM" id="CLU_550564_0_0_2"/>
    <dbReference type="OMA" id="HDPYGGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="42"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="471" mass="52557" checksum="3FFE81CAF4F97D5D" modified="2002-06-01" version="1">MKKDFTLDDIETTADIKIPDNPLDMVIGQDHAVEYAKLAAKQRRHLLLVGPPGVGKSMIAQGIAELLPKPREQIEVYHNPANPERPIVKVRTRDEVEKEDSDTLLSPEEVPEPVAEQLGFRCPSCGEYSDPDCRYCPRCGAQKYSARTYRATQYDRATSRQVAVIYERVGDRIRVVKPDSEGEEKKVLVPLDRKPFVQATGASETELLGDVKHCPWGGDEQLGEPPYKRVIPGAVHEAHEGVLFIDELAQLGPLQQHLLTAMQEKEYPITGKNPQSSGAAVRVEGVPCDFILVGACNIQDLPMILSPLRSRIQGEGYEVLMKTTMPDTEENRAKLAQFVAQEVERDGRIPHATREAVEEIVKEARRRARVIDGERDALTLRLRDLSGVVRLAGDLAVMEGAKYIEPKHVRAAIKKAKPVEDQIIEEYGSLEEGLRRDVATCSLSSPAAQQRVIQGDTTDSYYHQEDKRGYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-02-22" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ3</accession>
    <name>THIL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name type="ordered locus">MK0607</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXQ3"/>
    <dbReference type="SMR" id="Q8TXQ3"/>
    <dbReference type="STRING" id="190192.MK0607"/>
    <dbReference type="EnsemblBacteria" id="AAM01822">
        <property type="protein sequence ID" value="AAM01822"/>
        <property type="gene ID" value="MK0607"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0607"/>
    <dbReference type="HOGENOM" id="CLU_046964_1_1_2"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415640" description="Thiamine-monophosphate kinase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="127"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <sequence length="309" mass="33289" checksum="B1FDBF1B5985F202" modified="2002-06-01" version="1">MSSGFKRPSPRTWRPKEEEELIELIVEACPTEGPRVKAGDDDAAVLPDGTVVNFDAMTRSRHVPKELRDRDLGWKFVASVVSDVGAMGGEPSFFGFSVCLDDEVDVEQLVLGISEGLREFGVSLIGGDVVEGEELVLSGTCLGKLKGEPLLMSNARPGDVVAVTGPLGGPNAFVRAILNGMEPEETLYERFARPRPPVEVGVELARGGYRAAVTDISDGLLAEAEAIARRSGVAIEIHTWKVPVDEGVEEVAQEFDVDPVDLALEGGEDYEFLICGPEDVVKEFGLTTIGRVTEGEGVRIVRNPRARSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR2</accession>
    <name>Q8TXR2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01813">
        <property type="protein sequence ID" value="AAM01813"/>
        <property type="gene ID" value="MK0598"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0598"/>
    <dbReference type="HOGENOM" id="CLU_1656876_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="159" mass="17370" checksum="9A49E206C63BB268" modified="2002-06-01" version="1">MDVIRAILVTLALLYGVGWTSTVVNWTIPHEMVRMSAGIYQSTGATTQLTLNPYRGRATLAVAYDPAVLVKLGDRGKPVRLSPSSLRVGRVKVKSYGDAVVIHGPCSVRPYISAHGVRYSFDIIDTPGFSVYFNRSGYLVLVSHGKTTKIRVLPLIPLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-10-05" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS3</accession>
    <name>COFC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName evidence="1">LP guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofC</name>
        <name type="ordered locus">MK0587</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXS3"/>
    <dbReference type="SMR" id="Q8TXS3"/>
    <dbReference type="STRING" id="190192.MK0587"/>
    <dbReference type="PRIDE" id="Q8TXS3"/>
    <dbReference type="EnsemblBacteria" id="AAM01802">
        <property type="protein sequence ID" value="AAM01802"/>
        <property type="gene ID" value="MK0587"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0587"/>
    <dbReference type="HOGENOM" id="CLU_1425097_0_0_2"/>
    <dbReference type="OMA" id="FRVDYHG"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="PTHR40392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000398752" description="2-phospho-L-lactate guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <sequence length="190" mass="20960" checksum="7F03A8DAC114F380" modified="2002-06-01" version="1">MRFVVPFADRGDRKTRLSSCMDEETRERFALAMLRHVVRVLSKFGEVEVVTPDSSLSVPGTKVRRSDASLDELPLPDGEFGLVMSDLPLLSEEDVERALEGLKDADVVLCPSRRGGTSGVFVRKGVRFRPTFGGVSFPRNLRRAEKQGVEVAVVKSLGFFADVDEPEDLLDAALLGRREVAKIARSVVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS9</accession>
    <name>Q8TXS9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01796">
        <property type="protein sequence ID" value="AAM01796"/>
        <property type="gene ID" value="MK0581"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0581"/>
    <dbReference type="HOGENOM" id="CLU_121275_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016762">
        <property type="entry name" value="UCP019464_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09886">
        <property type="entry name" value="DUF2113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019464">
        <property type="entry name" value="UCP019464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03291">
        <property type="entry name" value="methan_mark_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="208" mass="24082" checksum="5236B2B50835BC4D" modified="2002-06-01" version="1">MYEHRGKRILVRASEKEAAELYYDITRHAATDLGLARTITAAVFHLDIEAPLYAAAVRTRPMLRPVTLGKVSHLELDDEEGTLKVSVAVERYFPDVIRTLQSVFGEDRVRHEERLEITIEPPEGMSPEHLEKLEELVVHDPRKKLAHRVYDFIERVRPEGFRVARYARFDQDFLYLASEGTLEDEWTDLLFELPGELDRVGERRGPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT6</accession>
    <name>Q8TXT6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator of the LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0574"/>
    <dbReference type="EnsemblBacteria" id="AAM01789">
        <property type="protein sequence ID" value="AAM01789"/>
        <property type="gene ID" value="MK0574"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0574"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.609"/>
    <dbReference type="HOGENOM" id="CLU_1100965_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="28008" checksum="B4B563C915EF89F8" modified="2002-06-01" version="1">MRLDVDVAIRVNGVLLTPEELELLLKLSEHGSMSKVAEEKGVTRSAVHKRIRNLEERLGCRLVESSPLGSYLTEHGRRIVIKYVNAKARLSRTETTVACSETVIEDVLAALGREHDVDVIVPPHEKMRRVEADVVVPDDPVIVFDRSDEAVGEPVVVRRTRLVRVGDGSGFVEVPGSAQRIYLTDLRNREEIRPKMRVSYYASALEAVRDGGMWTVVPEELAPEGDDPGPHYTVMALPLTRDGEDLLDVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT9</accession>
    <name>Q8TXT9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized metal-binding protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01786">
        <property type="protein sequence ID" value="AAM01786"/>
        <property type="gene ID" value="MK0571"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0571"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.606"/>
    <dbReference type="HOGENOM" id="CLU_091350_1_0_2"/>
    <dbReference type="OMA" id="GLCIGHD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014997">
        <property type="entry name" value="DUF1847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08901">
        <property type="entry name" value="DUF1847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="21520" checksum="BCCF2AF20EBA505A" modified="2002-06-01" version="1">MKPKCYRCPSPGDTCDRCGLADGDQMERTVESYRRDELFRSASEIEVEGYMEWTRVEEIAELCERMVWKSVGVAFCVGLAEEARALCEFLEGRGLEVYSVCCKVGGVPKSKLSLLELKPGDKVCNPRLQARLLNEIGTDLNVLVGLCVGHDIIFIEESEAPVTVAVVKDRRLAHCPALALTNRYYRRKLGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU5</accession>
    <name>Q8TXU5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01780">
        <property type="protein sequence ID" value="AAM01780"/>
        <property type="gene ID" value="MK0565"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0565"/>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="OMA" id="IFEVEIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="248" mass="27408" checksum="87CE056EA6034EF2" modified="2002-06-01" version="1">MTSRESLSDVAFVLPAHNEEGAVGKVVREIRRMYPDALIIVVDNASSDRTAEEAEKAGALVVREPVKGLGKAIKAGIQCALEHGARIILRTDSDGEFDPNCFAELIEAADDYDLVIGNRFSEGRPENVPLSHYLFNMILITLFWLFYGKILDVTCGCRVFSRELAREILKRDVDDGPAFDADTTSLAVSLGYSVKSVDVRLRGRISGQRKVAPTVIGKVLVGLRILFRVAVNRLRWRCETPSTLSRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY8</accession>
    <name>Q8TXY8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized ABC-type transporter, duplicated ATPase domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0520"/>
    <dbReference type="EnsemblBacteria" id="AAM01735">
        <property type="protein sequence ID" value="AAM01735"/>
        <property type="gene ID" value="MK0520"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0520"/>
    <dbReference type="HOGENOM" id="CLU_437379_0_0_2"/>
    <dbReference type="OMA" id="IAQMARY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="394"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="429"/>
            <end position="436"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="643" mass="73141" checksum="DCFA8343254BD2C3" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY9</accession>
    <name>Q8TXY9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">rRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lasT</name>
        <name evidence="3" type="ordered locus">MK0519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0519"/>
    <dbReference type="EnsemblBacteria" id="AAM01734">
        <property type="protein sequence ID" value="AAM01734"/>
        <property type="gene ID" value="MK0519"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0519"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.553"/>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="OMA" id="PIMNLSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="234" mass="26811" checksum="780C4D9BC0CC752B" modified="2002-06-01" version="1">MDVRVVLVEPKYDGNIGHVARVMKNFGVKELFLVRPRSELGEVAIARAMHALDLLENAVIVNTLEEAIEDVEAAIATTAVLASTPARNPMMPWEVREAFSDYEKIAVVFGPEDRGLRTWEVELCDATLHIPTSEEYRSMNLSHSVAVVLYELKKMEYVPERYGRAATKREKEVLVKSLDDLMKALNFDSTRRENVCTTFRRFLARARCTDKEIKALLWIFEKAKRRVLGVKRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY14</accession>
    <name>Q8TY14_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Archaea-specific enzyme related to ProFAR isomerase (HisA) and containing an additional uncharacterized domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0492</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0492"/>
    <dbReference type="EnsemblBacteria" id="AAM01707">
        <property type="protein sequence ID" value="AAM01707"/>
        <property type="gene ID" value="MK0492"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0492"/>
    <dbReference type="HOGENOM" id="CLU_791344_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01022">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="2">
        <location>
            <begin position="239"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="350" mass="38907" checksum="BDB7180ECBF28B0B" modified="2002-06-01" version="1">MGLGVALDIKEGKVVVRRSPDVREYIPISRRYDLPDDPHELATLLVERYDLDFVYVADLDAILRGEPADTSDALESLEKPVFVDVGACEPDLPSHAHRVIPTECYDDKSEYIEDLEEDESAVAGLDLNGSEILGPWDGVGDFLDTVVEVVYRRDPGVLVIDVGAVGSKEGPPYEAATSVGMYSTALIGGGVGHPEHVKLALGTPGVSGVILGTILFEGVDPMKLEQARREGKRLRVHHMGLEEEYLNLIKEGKKTVEGRVKDDKRARIKPGDKILFNRRLLVKVIDVREYDSFEEMLREEGLENVLPNVDSIEEGVEIYRRFYSSGKEKMFGVLAIEIEPIMDLWEGICD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY25</accession>
    <name>Q8TY25_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted NTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01696">
        <property type="protein sequence ID" value="AAM01696"/>
        <property type="gene ID" value="MK0481"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0481"/>
    <dbReference type="HOGENOM" id="CLU_825412_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="36731" checksum="BB9F768F94B4B156" modified="2002-06-01" version="1">MNCVGIAVSAIIFTSTAPEVPTNLRSKLLSSPSTTSNLSRPESSSSAILARWASDLRKGDMKGLLTQTSTVSSGNETRMVLRSSTTGITSKLTLNSRDNAESRGSMFAYMVKVLPTLLDAVVEEVNADKDHSETFRTEPWSGQPDEVVETRYIQLVRRLVGEGRNVVLVGEPGVGKSTTAIAACEKPVPIVLTFGGNLPVSILKTFVNTLRVTGPRRWISVDAVVVGDAVIEGKVRRLPLIDEVVVFSVTIVTWCLTRALKIPIIAMLATDKKYYAEARKILELAGFEVVEIKPDKHTVEEVLRAHGLQGSPRTDNPREELKRFSERSSNHSQSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY33</accession>
    <name>Q8TY33_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaE</name>
        <name evidence="2" type="ordered locus">MK0473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0473"/>
    <dbReference type="EnsemblBacteria" id="AAM01688">
        <property type="protein sequence ID" value="AAM01688"/>
        <property type="gene ID" value="MK0473"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0473"/>
    <dbReference type="HOGENOM" id="CLU_191930_0_0_2"/>
    <dbReference type="OMA" id="LETQIWF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011317">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09880">
        <property type="entry name" value="DUF2107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036535">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="95" mass="10244" checksum="CC64EE58EE6F5083" modified="2002-06-01" version="1">MHECEAILYTGAFMIILGTLGAAIGPARSDPVVKSLNLELATVGVCLVFLAFNHLLALITFIAVGAFTTPILMRAILRVEASERDREVLEGSERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY67</accession>
    <name>Q8TY67_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0438"/>
    <dbReference type="EnsemblBacteria" id="AAM01653">
        <property type="protein sequence ID" value="AAM01653"/>
        <property type="gene ID" value="MK0438"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0438"/>
    <dbReference type="HOGENOM" id="CLU_1387597_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="21651" checksum="827B8E9986557B22" modified="2002-06-01" version="1">MPFPMERFMLSITLWNTMTILITTFGGLIALTLDRLVTRYAPDVLRRSMNNPPYAPILVKLARKLGFDVRRYKEADVILTLKLGPLLIPAINGFAAGAFGMWVLQNFGPVFLLGAALLPHGVIEFPTLIIAGAMGVHLADYLIYRVRLHERWPHGNLEVPSWVIRNTAACIAGLTVAAYLEVHITPIVAGCVMKCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY79</accession>
    <name>Q8TY79_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidohydro-rel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01641">
        <property type="protein sequence ID" value="AAM01641"/>
        <property type="gene ID" value="MK0426"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0426"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.455"/>
    <dbReference type="HOGENOM" id="CLU_044590_6_3_2"/>
    <dbReference type="OMA" id="ICQQKGN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="3"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="26891" checksum="65BC88598081C2A7" modified="2002-06-01" version="1">MIIDVHNHLGEDIDSTVQTPQMLLARMEAAGVDIAVVFPFNDVDPGVCFSKANDRIAKACEEYDEFVGFCRVDPNYEERAVEEVERCIEELGLKGVKLHPRSQSFYPDDPEAVKVVEKAADLGVPVILHTARGEPFSDPVRVGKLAEEVPDVQLIMAHMGKELGYDAAIEVAENYENVYLEVSLVKDPKVIEKTAERVGDDRIIFGSDSPYGSPSVQLEIVKEADVNHDKILEENAANILGLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY90</accession>
    <name>RL3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl3</name>
        <name type="ordered locus">MK0415</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY90"/>
    <dbReference type="SMR" id="Q8TY90"/>
    <dbReference type="STRING" id="190192.MK0415"/>
    <dbReference type="EnsemblBacteria" id="AAM01630">
        <property type="protein sequence ID" value="AAM01630"/>
        <property type="gene ID" value="MK0415"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0415"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.444"/>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="OMA" id="HQRTEYN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_L3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_L3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="PTHR11363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000077211" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="361" mass="40166" checksum="9BB37AC142EDA3F8" modified="2002-06-01" version="1">MGRGGRRNPGRPRRGSLAFSPRKRASRPVPRIRSWPDEERVRVQGFAGYKAGMTHAIMIDDWPNSPTEGEEISVPVTILDAPPMYVAAIRAYAPTPDGYRCVTEAWAEIPEELEMDRVFTVPKDGEAGDLDKIEELVDEGIVEEIRVIVATQPKKAGVPKKKPDVMEYRIGGKDVRERFEYAVEILSEEIRAKDVFDEGEIVDVSAITKGKGFQGVVKRWGVTIQDRKTQRKQKGRHIGSIGPITPSRVRWTVPMAGQVGYHQRTEHNKRILKIGEDGEEVTPRGGFVNYGVVRGDYIMIHGTVPGPKKRLIRVRPAVRPPKNAPEGAPEILYISRTSQQGVRPKASEDEIVEQLGGPASA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA2</accession>
    <name>TRPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">MK0403</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text>The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYA2"/>
    <dbReference type="SMR" id="Q8TYA2"/>
    <dbReference type="STRING" id="190192.MK0403"/>
    <dbReference type="EnsemblBacteria" id="AAM01618">
        <property type="protein sequence ID" value="AAM01618"/>
        <property type="gene ID" value="MK0403"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0403"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.430"/>
    <dbReference type="HOGENOM" id="CLU_016734_0_4_2"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000098890" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <sequence length="275" mass="29942" checksum="C33E5D16A5BDBB9A" modified="2002-06-01" version="1">MSGLVLERIFEEAEGEGRGALIGYLTCGHPGLEETVSLARALRDGGVDILELGVPFSEPIADGPTIQKAVDEALRAGTTPWDCLEVAEEVSEFVPVVLLCYYNTLHANGFERYLSAAAEAGVSGIIVADMPVEESDEVHSVARDLEIDVIYLVAPSTTDERLKKIGERASGFVYVISRYGVTGARRDLSEDTLELVRWVRDHVDVPVAVGFGISERWHVEEVIAAGADGAIVGSAFIKEIHRSEDIAEAEERVRELAKELVEGARDGYRRRSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB4</accession>
    <name>Q8TYB4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted exosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0388"/>
    <dbReference type="EnsemblBacteria" id="AAM01603">
        <property type="protein sequence ID" value="AAM01603"/>
        <property type="gene ID" value="MK0388"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0388"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.414"/>
    <dbReference type="HOGENOM" id="CLU_131306_1_1_2"/>
    <dbReference type="OMA" id="RTFCYAT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="153" mass="17589" checksum="CC0F4DC85570A26B" modified="2002-06-01" version="1">MTLPVNSISARVIAHATEDEKKVLEALANVLGGVLEEGDVEPETFYAEGHHGNPITIFQVKIDRPKYIERVLEHWRENIPEEERRRVWSDIERRVDDKGNLYLRFDKQSAYKGELRISDADDVIRVKVNLESYPASREGGIKTLERLGIFSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD1</accession>
    <name>Q8TYD1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01586">
        <property type="protein sequence ID" value="AAM01586"/>
        <property type="gene ID" value="MK0371"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0371"/>
    <dbReference type="HOGENOM" id="CLU_1736430_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="150" mass="16838" checksum="64B7B08251CAA3C3" modified="2002-06-01" version="1">MGALEEFEWKLAEHDVPIPVRQDAVALYRVLLETVRIWGIEREEGVRESRSEVRARISCEGLDCAVLTKVGEDRPQLLLRTVLGPRLLAEVFERAHESGVRSFHFDLQGRGLRVEGEYDVGIVQIKVVGGGAGWELLEDLEKRGFSVTGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD6</accession>
    <name>HIS4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="1">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisA</name>
        <name type="ordered locus">MK0364</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYD6"/>
    <dbReference type="SMR" id="Q8TYD6"/>
    <dbReference type="STRING" id="190192.MK0364"/>
    <dbReference type="EnsemblBacteria" id="AAM01579">
        <property type="protein sequence ID" value="AAM01579"/>
        <property type="gene ID" value="MK0364"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0364"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.386"/>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142093" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <sequence length="242" mass="26223" checksum="7A36E1D41B844200" modified="2002-06-01" version="1">MAFVVIPSVDVVEGKCVQLVEGDPERRTFESDDPVETAHQWSEFFPWIHVVDVDAARGEGDNSDIIGRICEEVDAKVQVGGGIRSAERAEELIELGADRLIVGTVAFTDKDDFSKIVDVCHDHGIEVFVALDVNENHEVLVSGWKEDAGVTLEDAIERFNEVADGYLTTAVHVEGKEMGIDEKVVEKSTGATDLPVLYAGGIASIKDVKRAKEAGAYGVVIGTALYHGDIDPVALLDLMEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE5</accession>
    <name>Q8TYE5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl coenzyme A reductase</fullName>
            <shortName evidence="5">HMG-CoA reductase</shortName>
            <ecNumber evidence="5">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hmg1</name>
        <name evidence="6" type="ordered locus">MK0355</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-methylglutaryl-CoA + 2 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0355"/>
    <dbReference type="EnsemblBacteria" id="AAM01570">
        <property type="protein sequence ID" value="AAM01570"/>
        <property type="gene ID" value="MK0355"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0355"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.377"/>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="OMA" id="DDKMTRA"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023282">
        <property type="entry name" value="HMG_CoA_Rdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="PTHR10572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="SSF55035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="SSF56542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="410" mass="44038" checksum="4EDAAAE2C885D89D" modified="2002-06-01" version="1">MDEKKIEELVSKVVKGEIKFHEVEKYTDGDSEVATEVRRRALERLTGAKLEHLGKYTIDANRAMDKNIENMIGAVQVPVGIAGPLLVHGEYAEGEYYVPLATTEGALVASVNRGCSTITDSGGAHVRIVRDGMTRAPVFKLPSARKALEFCEWVRKHFDDIKEVAESTTRHGELLDIQEFVVGRHVFLRFEFDTKDAMGMNMVTIATEEAVNWIEEKYPDAKCVSASGNVCVDKKPSWLNNVLGRGRTVVAEVEVPRDIVEEKLKTTPEAMAEVNYRKNLVGSAAAGNIGFNAHHANIVAAIFIATGQDEAHAVDGSTGYTTMEVTEDGDLYASVTIPSLNVGTVGGGTGVETQRECLEILGVAGGGNPPGVNAKEFAEVVAAAVLAGELSLVAALAAGHLGKAHRLLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF9</accession>
    <name>Q8TYF9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0340"/>
    <dbReference type="EnsemblBacteria" id="AAM01555">
        <property type="protein sequence ID" value="AAM01555"/>
        <property type="gene ID" value="MK0340"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0340"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.361"/>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="OMA" id="KRGPHNC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01210">
        <property type="entry name" value="TIGR01210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="55"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="355" mass="40132" checksum="5C5E0BC17B570C5E" modified="2002-06-01" version="1">MDRSGYRGRDERPASRILWMKIEPGTDSVRVFTVRFRGRPDRYVAAWKQPDRVTGADRSVVIVLSTRGCRWFHETGGCVFCSYPLEGAGTRVPPELLIRQFERAYSRHRPDSPHGVKLFTSGSFLDPEEVPDEAVREILGRLAENELVTEVSFESRPEFITDERLELITEVLDGKSFEVGIGLETSDDELRASMNKGFSFREFAKAVKKVREYGGIPKAYVMLKLPFLSEAEAIRDAYMSCVDAHLAGCTRISICPTTVHRETVLEKAWRVGLYRPPWLWTCVEVARKVKGTLGDRVDLLVDTSGAGTPRGPSNCPRCSRRVARALREFTRTQDIGRLEGLKCSCLAVWRTHIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG2</accession>
    <name>Q8TYG2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted transcriptonal regulator consisting of wHTH DNA-binding domain and an uncharacterized domain conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0337</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0337"/>
    <dbReference type="EnsemblBacteria" id="AAM01552">
        <property type="protein sequence ID" value="AAM01552"/>
        <property type="gene ID" value="MK0337"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0337"/>
    <dbReference type="HOGENOM" id="CLU_073525_0_0_2"/>
    <dbReference type="OMA" id="IFIASRM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1360">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002846">
        <property type="entry name" value="NRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038982">
        <property type="entry name" value="NrpR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036984">
        <property type="entry name" value="NrpR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013668">
        <property type="entry name" value="RNase_R_HTH_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964">
        <property type="entry name" value="PTHR41964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08461">
        <property type="entry name" value="HTH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01995">
        <property type="entry name" value="NRD1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_12" evidence="2">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="NRD1_2" evidence="1">
        <location>
            <begin position="85"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="36553" checksum="C8272DE4D9DA2EEF" modified="2002-06-01" version="1">MNRLDVMILKILAEADRPMGSGRIAELIEERFGEKYSTRSIRYRLQKMEERGLIRRVRRNDRVVGAEITEWGLTMLKTETSSERVGMYISLIEEMAYRCSFDPEVMKGKVVCNISVVKREHLDDFLDAVVETYRAGISPSPLVAVKEDLSDHDVEVGEDEVAVLTVCSVTIDGVLINRGIPVTPVCGGLLYLEDGEPLGMQEYIKYEGSTVDPLHVFVAKGMTQVERVLETGTGLVPANVRYVPWAALEDVEHVERELEKADIRGIVDVPKVEGEILGIPLPPRSLGIVAYGGLVPVALLEERGISTRTYPTRTLVEYSSLEDARRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH0</accession>
    <name>Q8TYH0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01544">
        <property type="protein sequence ID" value="AAM01544"/>
        <property type="gene ID" value="MK0329"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0329"/>
    <dbReference type="HOGENOM" id="CLU_1754710_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="148" mass="15370" checksum="75E1F9CC2641DFB2" modified="2002-06-01" version="1">MPLLTSLVEGEWAPSHDPRMGGLGLAITGLLFLLPTMATAEVVWGPLVVTLVTALNAWYAVKALSSGPSPLVPLALLEPGLAVLCVVCEPVRTWLAGIPKLVAGALICLEGPVDRLFRVDEVSDDGDARADAGRSPIPTGSAAHLTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN1</accession>
    <name>Q8TYN1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted sugar kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0264"/>
    <dbReference type="EnsemblBacteria" id="AAM01481">
        <property type="protein sequence ID" value="AAM01481"/>
        <property type="gene ID" value="MK0264"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0264"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.267"/>
    <dbReference type="HOGENOM" id="CLU_979811_0_0_2"/>
    <dbReference type="OMA" id="HYIKPKI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="233" mass="25400" checksum="F3B57CB07E237424" modified="2002-06-01" version="1">MAAAAARVLEFLGEDEPRLITAGDLGEGDGSLEIYRRLREVDDDVLVIHYIKPKIDEIRRVDTSGKVIADAGGMYAAKAAGIGPEFHLFLPDPGELAFLADEKAHHPAYVRGFIAEVDEEEVPSLVRRAYEEDQVPDYMVVKGRRDHVVHRGEVVETVDEPLVEAMECIGGTGDTLTGIVAALIAAGFGTEEACVIGCRVNRRLGEIANATPTTRIHELTRAIPEALCEELRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN7</accession>
    <name>Q8TYN7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted Zn-ribbon-containing RNA-binding protein with a function in translation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0256"/>
    <dbReference type="EnsemblBacteria" id="AAM01473">
        <property type="protein sequence ID" value="AAM01473"/>
        <property type="gene ID" value="MK0256"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0256"/>
    <dbReference type="HOGENOM" id="CLU_196471_1_0_2"/>
    <dbReference type="OMA" id="KCGFEGP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="PTHR40733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HVO_2753_ZBP" evidence="1">
        <location>
            <begin position="15"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="64" mass="6855" checksum="3D21D24FBE904236" modified="2002-06-01" version="1">MTEAGRIERADYAVCSACGGPIAPGERAVSHPCPKCGEVVITRCQKCRRLGNRYQCPNCGFIGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP4</accession>
    <name>HDRA1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CoB--CoM heterodisulfide reductase iron-sulfur subunit A 1</fullName>
            <ecNumber evidence="5">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrA1</name>
        <name type="synonym">hdrA_1</name>
        <name type="ordered locus">MK0249</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 4 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="5"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000150058" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit A 1">
        <location>
            <begin position="1"/>
            <end position="669"/>
        </location>
    </feature>
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            <end position="270"/>
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            <begin position="287"/>
            <end position="318"/>
        </location>
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            <begin position="584"/>
            <end position="613"/>
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        <location>
            <begin position="617"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
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    </feature>
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        <location>
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        </ligand>
    </feature>
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        <location>
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        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="636"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="5">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LWL2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8TM02"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="669" mass="73742" checksum="C2D8D120157026E5" modified="2008-02-26" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ1</accession>
    <name>Q8TYQ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MK0241"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0241"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="288" mass="31022" checksum="7ED1A53FE2AF8DAC" modified="2002-06-01" version="1">MHAMLVALVASAPATLAACAIFKFLDGPLYEPVRGGTPRGVGVIPALIYAYLAPLTHGVPVAAHAVLGFVDDALGRTPTRFGVEVGHLARGTAMLVAFGWCWWKFHPITGLVAGFLPQPANIVDMQPRAFTFAALTATVATLPWWNPTLVSVAWAALIPYVLLDLKGRVMLGDAGNASVATALLLACTRGDPIGSIAFLISFTLAGAFYRFKVEPRLREYLEERLEIEDPTLMDAVWDVLTGGALGDLIKAKTFGRTEVPECGRLARLLGYRRLVLIGRSTVGERVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR2</accession>
    <name>Q8TYR2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0230</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="190192"/>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAM01447"/>
        <property type="gene ID" value="MK0230"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0230"/>
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    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="24010" checksum="39C6CF705BC382F8" modified="2002-06-01" version="1">MRMVVLFSGGPDSLLAALIACDEYDPEKIILATYDNGVLIGVEKAGINYSQVKRATDAEVEWRIFDIHGPFHRWGLRGLERRILRFGWNPVCLDCKFCMLFHALEKLEPDVIVTGDRESRKYPEQTPEAKAFWEEMCGEYGCEYFTPLWDWKKRGVYEELARRRVSVRGSEPKCMLAGSWKKPVSEEKVERYLEGLRKRLLGLRSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS5</accession>
    <name>Q8TYS5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01434">
        <property type="protein sequence ID" value="AAM01434"/>
        <property type="gene ID" value="MK0217"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0217"/>
    <dbReference type="HOGENOM" id="CLU_1122626_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="247" mass="27034" checksum="5BE074CACF104A79" modified="2002-06-01" version="1">MIRRALDVIGEQLDVEKLVEEARKDLTSALNRVTEYFGLEKLVVLDDSGKPIGIVGDGRMEGGLINAVYTIRESLGDVDTILVTSEDSTNYLALCLDEYVVVCESKMTLSDADIFMLKNDLGKVIDAAVSGQRVKSEIPFVVIDEHGLVILSNVDRCEEVGALISQLYRFVKDHVEGSIEWIKMTTGENKTLAVKPHNDFLIAFVVESDVEDADDACEETVKTLIEKVERAVAGSPSPPAEKGEGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS6</accession>
    <name>Q8TYS6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted regulator of Ras-like GTPase activity, member of the Roadblock/LC7/MglB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01433">
        <property type="protein sequence ID" value="AAM01433"/>
        <property type="gene ID" value="MK0216"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0216"/>
    <dbReference type="HOGENOM" id="CLU_118613_1_2_2"/>
    <dbReference type="OMA" id="NVGRIRY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Robl_LC7" evidence="1">
        <location>
            <begin position="2"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="115" mass="12202" checksum="34E585E063D25817" modified="2002-06-01" version="1">MIEKVLADLNRVEGVHGSLVVSSDGLIIAEAVPPDIDSEIVGAIATTVYGSGERVVDEMNLGDLEQMLIEATHGKVMIVDVGEDATLVLVVEPDANLGLIRLRAREAAEEIAKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT1</accession>
    <name>Q8TYT1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01428">
        <property type="protein sequence ID" value="AAM01428"/>
        <property type="gene ID" value="MK0211"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0211"/>
    <dbReference type="HOGENOM" id="CLU_1237926_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016736">
        <property type="entry name" value="MJ1481_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09873">
        <property type="entry name" value="DUF2100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018814">
        <property type="entry name" value="UCP018814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="223" mass="25348" checksum="EDDC813B6903381A" modified="2002-06-01" version="1">MTRVERLEELLSHAVSQRSGRIRVVTDVDELRLDEEVVRRIVHRAAELDHALVESAPEHVLRGERAQEVARALDDILRSVLELVGVEEETERDLDDLFKDSVLVVVRGRERKALRELVDAPIVQTGGPLVPEDYRKVNPNLPEKLPEGLVKSVERARRELEEYIRKSGAKRIVLVREEDDRVGEVLEEELPEVAEELGVDHEVIVVPDFTELSPSDLLSGRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU3</accession>
    <name>Q8TYU3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="10">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hemB</name>
        <name evidence="12" type="ordered locus">MK0198</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="10"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0198"/>
    <dbReference type="EnsemblBacteria" id="AAM01415">
        <property type="protein sequence ID" value="AAM01415"/>
        <property type="gene ID" value="MK0198"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0198"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.199"/>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_2"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="7">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="37654" checksum="340EB0A6B6CD5A33" modified="2002-06-01" version="1">MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPGQRYHSVETAVETVRELLDLGLRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGDDIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAEVALSHAEAGADIVAPSDMMDGRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQAIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYVDYRTAVLESLTCIKRAGADLILTYFAPEVVRWLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX4</accession>
    <name>Q8TYX4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01384">
        <property type="protein sequence ID" value="AAM01384"/>
        <property type="gene ID" value="MK0167"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0167"/>
    <dbReference type="HOGENOM" id="CLU_1329476_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="206" mass="23387" checksum="6DF8636E83007D55" modified="2002-06-01" version="1">MKTTERPELRVKILRAAAVSHEDISKEMDSYCAILGDPESIDDIVYEVMRLSNFEIIDNSIVKTRHGIVVWRYGVVLIKSTPEITSLIKRIVSDTNLMSVVFKAKDGNFLIAGPDIVLKKILSSARVKMKVKVTKRTRGIAFVETSTVVFDAMPGFLKDIINTLLGIEEEVFSILVLDVEDPEKFKKIIDKGPNVYWRRIEEDKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX5</accession>
    <name>Q8TYX5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01383">
        <property type="protein sequence ID" value="AAM01383"/>
        <property type="gene ID" value="MK0166"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0166"/>
    <dbReference type="HOGENOM" id="CLU_126374_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018645">
        <property type="entry name" value="DUF2072"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09845">
        <property type="entry name" value="DUF2072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="84" mass="9614" checksum="FBCAA18F9890DF42" modified="2002-06-01" version="1">MPHICIRCGEVYDKVTKELIRRGCLKCGCRLFKRVSEDDGDNPATIVVERDGVYTINIENIDDVVTVYKSGRFFIVLPEQKYGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX6</accession>
    <name>Q8TYX6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0165"/>
    <dbReference type="EnsemblBacteria" id="AAM01382">
        <property type="protein sequence ID" value="AAM01382"/>
        <property type="gene ID" value="MK0165"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0165"/>
    <dbReference type="HOGENOM" id="CLU_145183_0_0_2"/>
    <dbReference type="OMA" id="KLIEVTM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012017">
        <property type="entry name" value="UCP004977"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09846">
        <property type="entry name" value="DUF2073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004977">
        <property type="entry name" value="UCP004977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="120" mass="13331" checksum="EE4703EC5A41FF5B" modified="2002-06-01" version="1">MTDGEVKVEVLAKSALERMSTEEVVEYVIEKTRGGSVIVLEGQLDPETLTQIIRETMENVDLEEFTGVDIYVVPPKAKTDKGLFDRLLGRQSEEGMTVISPADVLKDMKKGKDFITLKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ6</accession>
    <name>Q8TYZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01362">
        <property type="protein sequence ID" value="AAM01362"/>
        <property type="gene ID" value="MK0145"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0145"/>
    <dbReference type="HOGENOM" id="CLU_121772_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018036">
        <property type="entry name" value="DUF429_subgr"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024051">
        <property type="entry name" value="DUF429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="170" mass="19099" checksum="220BA756BAD2F5D2" modified="2002-06-01" version="1">MYVVGVDLAAKPGNPAGFAVWREGEIVCWSESSDDERVLEVCRKAELVVFDAPLTESDRPFRRRDEIFRRYAPVLPLTFPGMRELSRRARSLVRRLELEVYETYPRAAERFIRLHGNPVDEHSRDAAICCAVGLAVLEGEAHVFGEPPKAALPKRELSVSVRALTAPPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ56</accession>
    <name>Q8TZ56_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate:ferredoxin oxidoreductase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">porB_1</name>
        <name evidence="2" type="ordered locus">MK0083</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0083"/>
    <dbReference type="EnsemblBacteria" id="AAM01300">
        <property type="protein sequence ID" value="AAM01300"/>
        <property type="gene ID" value="MK0083"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0083"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.84"/>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="OMA" id="ATGCMEV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="48"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="296" mass="32677" checksum="EF95AAF6DEA75D86" modified="2002-06-01" version="1">MGNKRRVPIPEEELMAPGHRACAGCGSALCARLCMKALGKDTVVVMPTGCIEVVTTPYPETAWEVPWIHVAFENAAAVASGIERALKALGKEDVTVAVLAGDGGTVDIGFQALSGMIERGHNIVYICYDNEAYMNTGVQRSGATPYFAATTTTPPGKIWKGEMRPKKDIPKIIAAHGAPYVATACVSHPQDLIKKVKKAKEVEGPAYVHVLCPCPPGWGHDSSETIEIAKLAVETGMWVLYEIENGEFRITYRPKERKPVKEYLKRQKRFQHLSEDDIEKIQRMVDEQWKELEGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC0</accession>
    <name>Q8TZC0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0015"/>
    <dbReference type="EnsemblBacteria" id="AAM01232">
        <property type="protein sequence ID" value="AAM01232"/>
        <property type="gene ID" value="MK0015"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0015"/>
    <dbReference type="HOGENOM" id="CLU_037423_2_0_2"/>
    <dbReference type="OMA" id="THHSKIL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="253" mass="27510" checksum="B86B99F238E50FA2" modified="2002-06-01" version="1">MRSSSKSVSVEGVLEVLEGLAPFDLAVEGDEVGLVAGDPSDSVDRVVVCLDLTPQLVRRLSPETLVISHHPVPGPLLERVRSPVIVFHSNWDAARAAEALAEWLGLEDVRKPDPLAAEGRFDGTLEDLLSRVEDALNPPEIRVVATKNRIHRVIVVSGFGLSTDRFVRLAAKEGADAVVSGDLTHRTAVVARVLGVTCVDATHARTELPGLKELAEELSKRLRVRVELRSPEHPVGDHYRAFQRRAARSQDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVE5</accession>
    <name>F1SVE5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type molybdate transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1521"/>
    <dbReference type="EnsemblBacteria" id="AAM02734">
        <property type="protein sequence ID" value="AAM02734"/>
        <property type="gene ID" value="MK1521"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1521"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1681"/>
    <dbReference type="HOGENOM" id="CLU_065520_3_1_2"/>
    <dbReference type="OMA" id="YGATGQF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="251" mass="27238" checksum="98E6CE7D51F0A69C" modified="2011-05-03" version="1">MRKVPIAIGILAVVVIAAAAGYYYTSSNQLTVFAAASLKKPLTKLAKQYEKEKGVKVALNFGPSGGLTAQILQGQKCDLFFSADWKYVVKLQKAGKTAKTKKFLKDYLVLVVSKTGEKKGIKSVKDITKPGVTVAVADPKAPVGEYTQRALEKLGIWDKIVKNGNLKARPGTVNQVATMVKNDQIDAGFVYRSVAVGFGLPIVQMFPHSLTGPIIWGAAVIKGGNEQLAEDFLNYCLEHIDEFKKYGWSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVN0</accession>
    <name>F1SVN0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type molybdate transport systems, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02735">
        <property type="protein sequence ID" value="AAM02735"/>
        <property type="gene ID" value="MK1522"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1522"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1682"/>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="OMA" id="MYSFIET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="49"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="261" mass="28008" checksum="82708667F22CE2AF" modified="2011-05-03" version="1">MIGRYEILSTLFPPLFFIFIVFPLVALVLNLTPQGIAEAFSDPYFWSATFNTILCAVAAAAAGVIIAIGFGYYHLFKRDSVIYRVADFLNDLPIALPHTVAGLALLLAFGRNYFGWLGENGLAFTLIAVVLAMLFVSYPLAARAVQAGVEELGRELVDVARTLGDEPPKAYVRVVVPALQEALLGGFLLGFSRSLSEFAAVIMFGGNLPGRTQVLASYVFTRAESGDLEVAVAASVFCMVLSLTMVALVRSVTGGLRRAEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>P58887</accession>
    <name>PYRK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrK</name>
        <name type="ordered locus">MK0564</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58887"/>
    <dbReference type="SMR" id="P58887"/>
    <dbReference type="STRING" id="190192.MK0564"/>
    <dbReference type="EnsemblBacteria" id="AAM01779">
        <property type="protein sequence ID" value="AAM01779"/>
        <property type="gene ID" value="MK0564"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0564"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.599"/>
    <dbReference type="HOGENOM" id="CLU_003827_1_1_2"/>
    <dbReference type="OMA" id="RYMKCGI"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000951">
        <property type="entry name" value="Ph_dOase_redase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00409">
        <property type="entry name" value="PHDIOXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000148376" description="Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <sequence length="262" mass="28783" checksum="184A7D1CE99B6761" modified="2002-07-11" version="1">MVSVKPIPAEVVENREECERTIVLRLRPERPIRWEPGQFLMLGISGVDEKPMAFSGGDDREFELTIEIVGPFTERCADLEPGDVVWVRGPYGKPFEVRGSRAVVVAGGTGVAPLVPLVERLRRARVHVTSVLGGPHADRLPRKDDLEKLSDELYVTTEDGSEGRKGFPTDVLEELVEKECPDVVYACGPEGMLVRVAEIAREHDVPCQVSVVRYVKCGEGICGSCALGKGLLVCRDGPVFWTEELEGTEFGGVRRDVTGKPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>P94948</accession>
    <name>Y008_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MK0008</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0008</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="245" first="386" last="391">
            <title>Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri.</title>
            <authorList>
                <person name="Klein A.R."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9151968"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.t01-1-00386.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0989.</text>
    </comment>
    <dbReference type="EMBL" id="Y10251">
        <property type="protein sequence ID" value="CAA71295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P94948"/>
    <dbReference type="SMR" id="P94948"/>
    <dbReference type="STRING" id="190192.MK0008"/>
    <dbReference type="EnsemblBacteria" id="AAM01225">
        <property type="protein sequence ID" value="AAM01225"/>
        <property type="gene ID" value="MK0008"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0008"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.8"/>
    <dbReference type="HOGENOM" id="CLU_151801_2_0_2"/>
    <dbReference type="OMA" id="VKACGPC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107133" description="Uncharacterized protein MK0008">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="121" mass="13324" checksum="F9BCB78024843746" modified="1997-05-01" version="1">MGTPEGIDVITVVISEAPYGQERAYTALRFALTALVEGEEVKIFLIEDGVFLGKKGQNPDEVPNYLELLEQCIEQGAEVKACGPCSKARGLSEEDFIEGVELATMHDLVNWVKESDNVIFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-26" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY9</accession>
    <name>PGK2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phosphoglycerate kinase</fullName>
            <shortName evidence="1">2PGK</shortName>
            <ecNumber evidence="1">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk2</name>
        <name type="ordered locus">MK0868</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TGY9"/>
    <dbReference type="STRING" id="190192.MK0868"/>
    <dbReference type="EnsemblBacteria" id="AAM02081">
        <property type="protein sequence ID" value="AAM02081"/>
        <property type="gene ID" value="MK0868"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0868"/>
    <dbReference type="HOGENOM" id="CLU_848909_0_0_2"/>
    <dbReference type="OMA" id="EMPFSKG"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156151" description="2-phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="25"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="327" mass="37960" checksum="45903A0DB51100D0" modified="2002-06-01" version="1">MSEKSSRKERDEKTEKETARQGKHRRIRVKSRHYEMPFSRGVLARSLTAIGVEPHKAYEIALKIKEELQDEGIEEISTDELADIIRTKLEEIDETLAERYELWRRIKKREEPIIVLIGGASGVGTSTIASEVGHRLGITNVIGTDAIREVMRRVLAEELYPTLYESSYTAWKRLRYEPAEDPVITGFLDHSEPVVVGIEGVVNRSINEGIHVIVEGVHIVPRLIKKEILNYPNVFVFMLAVEDEEAHKWRFYARSRDTKLSRPAERYLKYFEEIRRIHDFLVEDAEEHDIPVINNEHIDETVDQIVSYISSKLLKGERELSKSVSWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ1</accession>
    <name>Q8TGZ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Archaea-specific Superfamily II helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK0835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0835"/>
    <dbReference type="EnsemblBacteria" id="AAM02048">
        <property type="protein sequence ID" value="AAM02048"/>
        <property type="gene ID" value="MK0835"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0835"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.878"/>
    <dbReference type="HOGENOM" id="CLU_010611_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="8">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="220"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="413"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="818" mass="91715" checksum="C2136200A710817E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR7</accession>
    <name>PSTB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB</name>
        <name type="ordered locus">MK1687</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUR7"/>
    <dbReference type="SMR" id="Q8TUR7"/>
    <dbReference type="STRING" id="190192.MK1687"/>
    <dbReference type="EnsemblBacteria" id="AAM02900">
        <property type="protein sequence ID" value="AAM02900"/>
        <property type="gene ID" value="MK1687"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1687"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1852"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="VKHALQG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092947" description="Phosphate import ATP-binding protein PstB">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="254" mass="28874" checksum="F066206B6A840A10" modified="2002-06-01" version="1">MTAFEVRDLRVYYGEEEALKGITLDIPEKKITTIIGPSGCGKSTFLRCLNLMIKEIPYARTEGEVIFDGENVLEYEDEADIIAHRRRVGTVFQHPNPFPWMSIYDNVAYGLRLMGMDEDEIEDRVYEALEKAALLDQVEDRLDDPASALSGGQQQRLCIARALAMRPEVLLMDEPTSDLDPIATRKIEETVMELKGEVTIVFVTHLLPQAYRIGDYTAFFLHGELVEANSTEKLFTDPQDERTKEYIEVEFGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS0</accession>
    <name>Q8TUS0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02897">
        <property type="protein sequence ID" value="AAM02897"/>
        <property type="gene ID" value="MK1684"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1684"/>
    <dbReference type="HOGENOM" id="CLU_1324031_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="43"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="22852" checksum="9CF115C27CDDFC98" modified="2002-06-01" version="1">MEGTRQRVFYGLLSRVYERSPLSLPKHLVKEILRDLKGCCRVLDVGCATGYLTRKLAAVCDRVVGVDINRKMVEASQSRNRLPNVKFVRADAHNLPFPDACFDGIVLSEILQHLDVIRALKEVDRVAARGCRMAVVLPDPTSRAARVATRLIHVFTGNNAWVSPGTVVGIMRDMDWKLVRFQSAGKRVLITMEKRGAGDAGRSGPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT8</accession>
    <name>Q8TUT8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="1">5.3.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpiA</name>
        <name evidence="3" type="ordered locus">MK1665</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.3.1.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1665"/>
    <dbReference type="EnsemblBacteria" id="AAM02878">
        <property type="protein sequence ID" value="AAM02878"/>
        <property type="gene ID" value="MK1665"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1665"/>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_2"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="212" mass="23063" checksum="3608FC8AB1D9AE9B" modified="2002-06-01" version="1">MSGKRNAARKAAEEVCRRGYEVIGVGAGTTVEAFLEELVKREADVLVFTTIPGTIDACRRIGLNVTTIPPEELPVAIDGADAVDPDGNLLKGGGACHSLEKAIDYTADEFWVVVDESKLVENLWELPVPVEVLRGCYELTVRTLEEFGEVRPRTCDEKYGPVVSDSGNPIVDLHVDDWDPAELERELNSVPGVVECGVFPGDKVDRIIVGRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX2</accession>
    <name>ARGB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">MK1631</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUX2"/>
    <dbReference type="SMR" id="Q8TUX2"/>
    <dbReference type="STRING" id="190192.MK1631"/>
    <dbReference type="EnsemblBacteria" id="AAM02844">
        <property type="protein sequence ID" value="AAM02844"/>
        <property type="gene ID" value="MK1631"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1631"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1794"/>
    <dbReference type="HOGENOM" id="CLU_053680_1_0_2"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112695" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="5"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <sequence length="246" mass="26086" checksum="D550515A74C373FB" modified="2002-06-01" version="1">MEVIKVGGEVLDRVEDLARVIDDSILVHGGGPEVSDVMERMGLEPRFVRGLRVTDRETLQVVMMVLAGLVNKRLVAELRSEGINALGLSGVDGGLLIAEKRSEVVDGEEVDLGYVGDVKRVNAELLESLLDAGYVPVVAPLGAGEDGTVYNVNADTAAGAIAGAVRADRLVLLTDVPGVLEDLDDPETLIERVRPEDVEELEEKGIVTGGMVPKLEAAKMAVEAGCREAVITNLEGLLEGRGTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX8</accession>
    <name>Q8TUX8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ni,Fe-hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hyaD_2</name>
        <name evidence="1" type="ordered locus">MK1624</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1624"/>
    <dbReference type="EnsemblBacteria" id="AAM02837">
        <property type="protein sequence ID" value="AAM02837"/>
        <property type="gene ID" value="MK1624"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1624"/>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="OMA" id="NWTLSEP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="189" mass="20476" checksum="3721C5E1F8AC9136" modified="2002-06-01" version="1">MSKDSVRAASTGHTHGAGVGLLRFLQRRVLIVGCGNELFGDDGFGPAVIKEIERRGWEHPDVEILDAGAGAPQNVFSLIDEDSKVEYMVVVDAVDVGAEPGTLLEFGPEDLDPNCRIVPVDAHGWSIESALLDLNERIGIDFRILGCQVKELPIPEVQPGLSDPVRKAVPKAADRAIELAERYLGEKTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV61</accession>
    <name>SYH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="1">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisS</name>
        <name type="ordered locus">MK1538</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV61"/>
    <dbReference type="SMR" id="Q8TV61"/>
    <dbReference type="STRING" id="190192.MK1538"/>
    <dbReference type="EnsemblBacteria" id="AAM02751">
        <property type="protein sequence ID" value="AAM02751"/>
        <property type="gene ID" value="MK1538"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1538"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1699"/>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136313" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <sequence length="421" mass="47470" checksum="EE14F5F2C3F14D25" modified="2002-06-01" version="1">MGVTLMKVERPKGTRDFTPEEMRARRWLERYLLDVFRSYGYEEVLTPTFEHAKLFEEKSGEEILEEMYVFKDKKGRKLALRPEMTAPVVRFYNAELKTRPHPLKLAYIVNCFRYEQPQRGRWREFWQAGVEVFGSDRPEADVEVIELTYRIFDGILPSGAFEVRVGHLGILRGLLEEYGIGEDVQNKVAHLVDKGELDEVKTLLPAEPAEKALAVVTARSESEVEEAVSGKERAERALENLMEISDALREAGVDHELDFSVARGLDYYTGMVFEIHVPELGGGSQCAGGGRYDDLVKELGGPDVPAVGMAIGFDRLLLAAELYDRIPDGVEETRALLIPLVRSGKIWEIAAKLRKLGWVVNVEVSGKHIRKALSLADSLEYDYAVIVGERELKEGYVSVKNLKTGVQEEVPLDQLERAAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV76</accession>
    <name>Q8TV76_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 6">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dph2</name>
        <name evidence="7" type="ordered locus">MK1517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1517"/>
    <dbReference type="EnsemblBacteria" id="AAM02730">
        <property type="protein sequence ID" value="AAM02730"/>
        <property type="gene ID" value="MK1517"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1517"/>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="PTHR10762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="343" mass="38410" checksum="B82E89D97E629FDB" modified="2002-06-01" version="1">MTRCTSAESYDEDEVPRVVESLNVEGRVLVQAPDGLKRIAREVADVLEARGCEVELDAGRVFGACDITPGTVVRTDHIVHIGHYPIPEVERQIRELGATVHFLPTHGRKRVEPWMAEEAVEVLHNLGIDRVNVCATAQYVADLELVSEILRESGIEPVIRPGDGRRVATPGLVLGCNFSALDTSLPTIVVCSGSFHPAGVAIRTNRPVVQIDPTKGVLDSEDVEDVIRRILAVRLSKIREFNREEGFEIVESTALGQRRPDVVRVLDRPTWRLRYLTEDALRSLRRRQCVFCGCPRVPVDEAARFKRWVLLNPAELAAVRAGWKEYRLDEIPTPEDVLNALRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV99</accession>
    <name>Q8TV99_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hypE_1</name>
        <name evidence="3" type="ordered locus">MK1493</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1493"/>
    <dbReference type="EnsemblBacteria" id="AAM02706">
        <property type="protein sequence ID" value="AAM02706"/>
        <property type="gene ID" value="MK1493"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1493"/>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="OMA" id="IGGDMVI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="115"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="242"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="452" mass="49419" checksum="62EA051E3691D788" modified="2002-06-01" version="1">MDLEGLSLHMLEAGASEDEIRELLTDLVKIWKRDWSEDEIREFVDAVLEEVHHVRKAHFLGGRVGDILRPPESGVGMGEMGVGSRGEGDFFVHELLTRLAAKASEGALVSPEERDDAGAVRIDPDEVILVSAVDGMHSRLSEFPFLAGFHATRAAMRDVLVNGARPRGLLVDLHLADDGDVGRLFDFTAGVTAVGAATGVPILAGSTLRVGGDMVLGRRLVAGVACVGTARPDELTPRRDAEPGDLIVLTEGAGGGTISTTAIYHGYYDVVEETLNVDFVRAVEALREENLLSEVHAMTDITNGGIRGDATEISETAGVRLVFDEETVRSLVNDRVLRMLDELDIDYLGLSLDMLMVIAPEDVAERCVEALNGVGVRADVVGRVEEGSGVVLERDGEEVELDVKFRETAYTKVKRVIGEEHPEEFEEMKERVRRAYEEAERKLRWVLERLGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-06" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC0</accession>
    <name>RS4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4</name>
        <name type="ordered locus">MK1472</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVC0"/>
    <dbReference type="SMR" id="Q8TVC0"/>
    <dbReference type="STRING" id="190192.MK1472"/>
    <dbReference type="PRIDE" id="Q8TVC0"/>
    <dbReference type="EnsemblBacteria" id="AAM02685">
        <property type="protein sequence ID" value="AAM02685"/>
        <property type="gene ID" value="MK1472"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1472"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1628"/>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="OMA" id="ARQFITH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_S4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_S4/S9_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_S4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132509" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="106"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="186" mass="22370" checksum="64008CE0AE7603D5" modified="2002-06-01" version="1">MGDPKRPRKKYETPRHPWEAERLEYERKLMRKYGLRRKKELWRHQTQLKRWRERAKELMARTDPEAQREREALFRKLYDLGILDKKPEEATLDDILRLTVEDVLERRLQTIVYRKGLAKTPLQARQLVVHRHIAIGDRIVTVPSYLVSREEEEEVDYSPYSPLKDEDHPIRCEARGESPEETAAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD0</accession>
    <name>Q8TVD0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted RNA-binding protein, contains THUMP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1462</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1462"/>
    <dbReference type="EnsemblBacteria" id="AAM02675">
        <property type="protein sequence ID" value="AAM02675"/>
        <property type="gene ID" value="MK1462"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1462"/>
    <dbReference type="HOGENOM" id="CLU_795999_0_0_2"/>
    <dbReference type="OMA" id="ESRYQIQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd11718">
        <property type="entry name" value="THUMP_SPOUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041730">
        <property type="entry name" value="MJ0421-like_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025849">
        <property type="entry name" value="SPOUT_MTase_fused_to_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14419">
        <property type="entry name" value="SPOUT_MTase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="42"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="348" mass="38672" checksum="687B58A318EA00D9" modified="2002-06-01" version="1">MFLVKTQRNLEDVAAAMIEEETGAEAKPRPFGYLGLVIVTGHVTKDELEKIPEVERAIPVEAECRADPKEIAETAAELAETKISEDETFAVRTIRRGEHDFTSVDVNVEAGDAVRKATGASVDLDDPDKIVWVEILRDRAYLAVLPGEEEWKKLPPGKPEADPLLAKLELAQIPYLGDPRAAYSMGERIGRAVQGFELKSYIVTPYEPVNAVELLHFLRGLRDGVKSRMDVQRESYGREFRRTELLLYDLYQLIRLKRDALIIGTDPKGDPYTEIRKTLGEALREADEVVVLAGSRVGLPRGVLRACDFVVDLCPGVTFATEHVVPSVVTALVDSYLEAEGSEDREGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD3</accession>
    <name>Q8TVD3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR_Cas6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02672">
        <property type="protein sequence ID" value="AAM02672"/>
        <property type="gene ID" value="MK1459"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1459"/>
    <dbReference type="HOGENOM" id="CLU_1010527_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019267">
        <property type="entry name" value="CRISPR-assoc_Cas6_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10040">
        <property type="entry name" value="CRISPR_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRISPR_Cas6" evidence="1">
        <location>
            <begin position="146"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="275" mass="31735" checksum="2D4D3D263BED8B11" modified="2002-06-01" version="1">MRYKPLRFELECVEPGALPRFKGFAVRGMLLRRLKERFPGFVRRFLYGNHPIPAVTQVPPLTDERMVDVGDIVSFRLNLFGDAVDRDYEIILALVDGELRLGSARFVLRRVEHAETGEPVWDESAYRCVKPEKLDAREPVGRYLVVTFTTPTYIVHDGKPRVPKFHMIVRNAARKFTMLHRRFGLEGLTRRQARNIIEWAERAETIRMDYRFETLERRSARLGRHVFRTIVGTFVYELPPEPPDRVGEVLAFAEVYGVGKFNTAGLGRFVLGEAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE9</accession>
    <name>GGR1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglycerophospholipid reductase 1</fullName>
            <shortName evidence="1">DGGGPL reductase 1</shortName>
            <ecNumber evidence="1">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2,3-bis-O-geranylgeranylglyceryl phosphate reductase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranyl reductase 1</fullName>
            <shortName evidence="1">GGR 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1443</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE9"/>
    <dbReference type="SMR" id="Q8TVE9"/>
    <dbReference type="STRING" id="190192.MK1443"/>
    <dbReference type="EnsemblBacteria" id="AAM02656">
        <property type="protein sequence ID" value="AAM02656"/>
        <property type="gene ID" value="MK1443"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1443"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1599"/>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OMA" id="GMCRREV"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000351458" description="Digeranylgeranylglycerophospholipid reductase 1">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="200"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="283"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="287"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <sequence length="375" mass="40496" checksum="B58A1922673E20A6" modified="2002-06-01" version="1">MEFDVVVVGAGPAGSVAAWAAAEAGCDVLILERKAEIGVPKQCAEGISARALEEVGIRPDDGWIAAEIERGILSLPSGSKFEVEVEGYVLERRVFDKWLVVRAVEAGAEVELLAHARRALLDEGRVVGVEYEGEDGVHEVRARIVIAADGIESRIGRTAGLVPQLEPDHICTCAQYEVVGDRYDPKAFMIHFDPERIPGGYAWVFPKGENRANVGVGIRGSESSPGLALKTLDELVEGPLSELVAGTPVEVNVGGVPVCGPVERTYGDGILLVGDAARQVNPLTGGGLNTALICGRIAGEVAVEAIEEDDTSASFLKRYQDRWEEEFRDTFECAREVAEMLPELDLKEVVEFLSSVENLEEMLRTSGILEDVWWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF4</accession>
    <name>Q8TVF4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02651">
        <property type="protein sequence ID" value="AAM02651"/>
        <property type="gene ID" value="MK1438"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1438"/>
    <dbReference type="HOGENOM" id="CLU_1056065_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="263" mass="29220" checksum="B3B2EE9E85BB2566" modified="2002-06-01" version="1">MGGSERSVPVLLPFLEDPLTMFPSRVIIPSTRYIMEVVRDLRAPWRGEVLDAGAGCGSFALTVAALGPYTVYAVEPDPEHSAALSANVSANRDVLLGDVLPLECSIEDFRRPVDEVLTDPPWGLRSGISRTPDLEFVLSFLDACVDVLRPKTGRLVTRCPPEFIDDIVDHMSERGYLLDRVKRRHKAAVLVLRSEDHRDYYDDRESAVAAAGGQVLVAWEGGELDPEADRYSLVTPYESGWHVWEVPNTGRIREFLRGFLKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG2</accession>
    <name>Q8TVG2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1427</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1427"/>
    <dbReference type="EnsemblBacteria" id="AAM02640">
        <property type="protein sequence ID" value="AAM02640"/>
        <property type="gene ID" value="MK1427"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1427"/>
    <dbReference type="HOGENOM" id="CLU_061501_0_0_2"/>
    <dbReference type="OMA" id="CIFCYSN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="279" mass="31523" checksum="5CD1E510DAB7E711" modified="2002-06-01" version="1">MTRLSLPTSRHCEGCPGAEGEGPHHPTLELTTSCPYKCPHCYARYAENVGVVVKPGLYGEPQGCLTVSQYGEPTVLGRELIDVLEMVRETGLFDRIDLQTRGYRPDLAPKLSEICDLVMVSIDVTDPDVHRRLHGVGPERTLRFAVNTDRPVIRSLYLPGINDDLPQGLADTEIEPAEVFVQPLIPFGKAVENLKRIGLRDHYNVVGSLLNWAEKFEEFGFDVRFPACWVDSLERLKERMEEELGFVDLRNVRYSPDPGTPAPERRFTPLRELLDELVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-03-07" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG5</accession>
    <name>SYV_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">MK1424</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVG5"/>
    <dbReference type="SMR" id="Q8TVG5"/>
    <dbReference type="STRING" id="190192.MK1424"/>
    <dbReference type="PRIDE" id="Q8TVG5"/>
    <dbReference type="EnsemblBacteria" id="AAM02637">
        <property type="protein sequence ID" value="AAM02637"/>
        <property type="gene ID" value="MK1424"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1424"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1580"/>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000224624" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="552"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="914" mass="105317" checksum="3B24088E8E9DF06D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI1</accession>
    <name>DHQS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate synthase</fullName>
            <shortName evidence="1">DHQ synthase</shortName>
            <ecNumber evidence="1">1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroB'</name>
        <name type="ordered locus">MK1408</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + H2O + NAD(+) = 3-dehydroquinate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVI1"/>
    <dbReference type="SMR" id="Q8TVI1"/>
    <dbReference type="STRING" id="190192.MK1408"/>
    <dbReference type="EnsemblBacteria" id="AAM02621">
        <property type="protein sequence ID" value="AAM02621"/>
        <property type="gene ID" value="MK1408"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1408"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1563"/>
    <dbReference type="HOGENOM" id="CLU_056379_0_0_2"/>
    <dbReference type="OMA" id="PMFRVAL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQ_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="entry name" value="PTHR33563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000058770" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01244"/>
        </source>
    </evidence>
    <sequence length="402" mass="44135" checksum="31F2353E861312FA" modified="2002-06-01" version="1">MRPKQVWVSVAFEGEWNEKKPYVTESIEAGVDVIVCLPEDVERVKELGNVKVAVPLMPESPGSPDLALEELDAIDADVVIVGKGGEGDGSIDLPDDISESIDAALIEKARDRGFEVAEYVEILDKPYERFAAEIAKNVGPDYVIAIGRDWKIIPLENLIAELQGEKTQLIAGARDAEEARIAFETLEVGSDGVLLDAERIDPSEIKKTAEIAERAAAERFELVAVEVKEVKPIGKGDRVCVDTCSLMSEGEGMLVGSTSRGMFLIHSESLENPYVEPRPFRVNAGPVHAYIRVPGGKTKYLAELRPGDEVLIVDTEGRTRAAVVGRLKIERRPLMLIRAEYEGVEIQTIVQNAETIHLVREDGEPVSVVDLKPGDKVLAYVETEEGKGRHFGMEVEETIVEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ5</accession>
    <name>PRIL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priL</name>
        <name type="synonym">pri2</name>
        <name type="synonym">priB</name>
        <name type="ordered locus">MK1394</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVJ5"/>
    <dbReference type="SMR" id="Q8TVJ5"/>
    <dbReference type="STRING" id="190192.MK1394"/>
    <dbReference type="EnsemblBacteria" id="AAM02607">
        <property type="protein sequence ID" value="AAM02607"/>
        <property type="gene ID" value="MK1394"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1394"/>
    <dbReference type="HOGENOM" id="CLU_677258_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="PTHR10537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000046781" description="DNA primase large subunit PriL">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <sequence length="406" mass="46873" checksum="9F08B3B42B4030D5" modified="2002-06-01" version="1">MRSWFPHAPTVSVPEIEPEEALSYRDEIRTRVRSYAFGLDDWRAKVKTRRAYLRKVRDEDESEILRVYGALLTVAAAGPRPVRHLIAEGESAMAETALYALRHEDESAMSYPEERVLSDLYLWDVRVLERDLGLYAVHFSFPLIHRSPEGQKLKVLVWERASLEKVLKEVRSNRVLGVRVLDPSGWNDVWICPRCGATRRGGTGDLEQDHARRCSNCRGRMRKPDPNLLEMLKEPEGYLIMHFKTLARTFREDVRRLVVSDIESRDDVEDEELREFCLKLFPKVRKRLERVEKGAGGRFPPCIRELLRRAQEGENLPHEARFALAAFLVNVGWDVDRVVEVFSNLPDFDEERTQYQVRHIAGEVGGGTRYLPPNCDKMKAWGLCPGKDCGVKNPLAYYRRPRADDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK6</accession>
    <name>Q8TVK6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1383</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1383"/>
    <dbReference type="EnsemblBacteria" id="AAM02596">
        <property type="protein sequence ID" value="AAM02596"/>
        <property type="gene ID" value="MK1383"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1383"/>
    <dbReference type="HOGENOM" id="CLU_111441_1_0_2"/>
    <dbReference type="OMA" id="RETMICG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="195" mass="20941" checksum="2956AA7C8EBDC963" modified="2002-06-01" version="1">MSPLNRKELLRRVLKEALATGRYMALGSMDGVLASMGAVLSVVARGGSAQDAASAGLSVAVALALSNGFGSYLGEKIEELREIRELERQMIMKRGGLEHTRVHDLARIRIYTSVVSHGGSSFMASMVPILPVLIIKDPKWSVIACTCVSGIFLFLLGVYLGRLCKERKKDLILRGCETAAIGGLVGLVTHFMGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK7</accession>
    <name>Q8TVK7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1382"/>
    <dbReference type="EnsemblBacteria" id="AAM02595">
        <property type="protein sequence ID" value="AAM02595"/>
        <property type="gene ID" value="MK1382"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1382"/>
    <dbReference type="HOGENOM" id="CLU_011543_3_2_2"/>
    <dbReference type="OMA" id="PESYNWS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="208"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="514" mass="57419" checksum="18E70E00249E3305" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP8</accession>
    <name>Q8TVP8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02553">
        <property type="protein sequence ID" value="AAM02553"/>
        <property type="gene ID" value="MK1340"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1340"/>
    <dbReference type="HOGENOM" id="CLU_996089_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="279" mass="30676" checksum="797627394215B805" modified="2002-06-01" version="1">MRLPREEVLTRVLDANAVDLYDVEMLFDASYPGGKVRAYSVLELHPPREGALLHPQLRPRRHVLGPGEVRPREALIWGWAGNEPVVRLLRPAVRVIERDGRRSIECAVTGSAWVGEREARVALRAEVDPAHPAAGDPRGSRRPLGPDILPHDPSRDPGPSASASWCAPTTVASWGRTSDPRGPRGGPPVRIEVPRRDGRRRGSGAADLLVHRWYVTPASPDWTLEPATDLTWIATLDDRFGRWTPILELRLTADPILPVHPRLVPDGLIDVPLGVSSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU8</accession>
    <name>Q8TVU8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02503">
        <property type="protein sequence ID" value="AAM02503"/>
        <property type="gene ID" value="MK1290"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1290"/>
    <dbReference type="HOGENOM" id="CLU_1109507_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="250" mass="28325" checksum="84D37F21882E86DE" modified="2002-06-01" version="1">MPRRGEPVDTEGRLRETIGRYHGVVLDTNAFAALAKAFVDDICETPVDNVVYAFFLEVKRMMGENFLLYSYEELRAEYAKRLEDLRDYDGQEVCYACPILSELLGPTVRGLCYLFKGHEDVSVHDDVLSRPEYSEQLERLYRGLRRGGYAGLPESLRGFKCNFEEGQVGDKHVDVANVMVAAVKGSVLVTSDRGIRDACSELKPRCLCVYFNPGYVDDPVREEFEFELVPRPGGLPVGLRYRKGIGKFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW1</accession>
    <name>Q8TVW1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein specific for M.kandleri, MK-14 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1277</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02490">
        <property type="protein sequence ID" value="AAM02490"/>
        <property type="gene ID" value="MK1277"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1277"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1393"/>
    <dbReference type="HOGENOM" id="CLU_1131615_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="245" mass="26908" checksum="22F62604B3EEFB43" modified="2002-06-01" version="1">MFPPCRIMHVVLWSALWSLLWVVILLAVYRRSGRPSNRWISTIAGRRWFQDLRSRVKEVLEAVETAEELVDTTFEGKGAPPELDRRLADVLRVPDREVDWRTGLGIEYLGKSAGDRFALELALELTGSEEAVRELVEDGYDVLPFDVRLEGPTGTVSARGVVVPLGRSDGTPVVAVVGVGRRGTGRIEPVDSELFVFGGLVSSRRTFSSVLRTFGELLPRGHADRRGARHCPRPPGGGGGAREPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY1</accession>
    <name>Q8TVY1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02470">
        <property type="protein sequence ID" value="AAM02470"/>
        <property type="gene ID" value="MK1257"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1257"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1365"/>
    <dbReference type="HOGENOM" id="CLU_910935_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="35785" checksum="7B9ED1DF504F940F" modified="2002-06-01" version="1">MGKYVIVFREPAEPIYWRAKWLLAAVKCYRENKECRDYIHKEIGIHKDVKVSQNDIETKILVEAFIKTDENIKQGDINTILVALLNQSLELMAKYIAERLMWLIGIWKKGKEGYYVILNPNDAKKVRRCVYKSHNVIDILDHLKKTLKDIDGIAEKLCDEEYSNNCRLSELADEISKFIKKYCNILEKMEDAYYDKYSDNIEKGELWYSKNDVDKAIEVVEKFDRSIVSLAIDVSAHIPGILTDMKWILSEIRKAGCVPENEIKKKALDKRKVNEKELQEIINFAVENSWIERKDGELKWMKNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY3</accession>
    <name>Q8TVY3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing PIN domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1255</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02468">
        <property type="protein sequence ID" value="AAM02468"/>
        <property type="gene ID" value="MK1255"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1255"/>
    <dbReference type="HOGENOM" id="CLU_1438121_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13470">
        <property type="entry name" value="PIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="21442" checksum="B828BAE98D9B247B" modified="2002-06-01" version="1">MRVMLDTNVVLRAVEELLSPSIPEVTRPVLISEGGRGVRRRLIGLKPSSREQRVESLYTFTFSLLGCLDVTLVVGPRLMKEYEKNLAGESFFNRCWNLRGVLQLLEEKCKWVDPDEELVELCRKAHPKDDPADAEHAAVCLQTKAVLVTLDVSHFSQMAEELRREGLALKWRTPFDFLQELGVFKCRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY5</accession>
    <name>Q8TVY5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri, MK-28 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02466">
        <property type="protein sequence ID" value="AAM02466"/>
        <property type="gene ID" value="MK1253"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1253"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1361"/>
    <dbReference type="HOGENOM" id="CLU_278575_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1100"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1134" mass="124533" checksum="AE9719353CCE575E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ7</accession>
    <name>Q8TVZ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1241"/>
    <dbReference type="EnsemblBacteria" id="AAM02454">
        <property type="protein sequence ID" value="AAM02454"/>
        <property type="gene ID" value="MK1241"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1241"/>
    <dbReference type="HOGENOM" id="CLU_2177890_0_0_2"/>
    <dbReference type="OMA" id="FARAPEI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitro_FeMo-Co" evidence="1">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="109" mass="11605" checksum="CC4A926C50034EFA" modified="2002-06-01" version="1">MRVAVMVEGDGVSSRFARAPEIRVYEVQDDDVELVESTSNPVANLPRGAGRQILTVLTELNVEATVAARYGPNALQGLKAQGIKAYVAEPGTDPEEAALKAARGELPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW22</accession>
    <name>Q8TW22_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">leuB_2</name>
        <name evidence="5" type="ordered locus">MK1215</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1215"/>
    <dbReference type="EnsemblBacteria" id="AAM02428">
        <property type="protein sequence ID" value="AAM02428"/>
        <property type="gene ID" value="MK1215"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1215"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1317"/>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="4">
        <location>
            <begin position="6"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="35909" checksum="EA91DF4F5D6C4412" modified="2002-06-01" version="1">MNASPTVVVIPGDGIGPEVIDAALKVVRAVLGDELEIVEEQAGYSLWKKRGVTIEDETIERCREADAMLFGACTTPEDPEAKSPIVTLRKELGLYANVRPARSWPVPRPVDTEFDLVIVRENTEGLYTGCECEIHDGVTVALRKISEEGTRRVAEVACDLAEERSGRVTIVHKANVLKLTCGTFKRVAAETVERRGLEWDDEYVDAAAYKLVREPDSFDVILTSNLFGDILSDLAAGLMGSLGLAPSANLGDDAALFEPVHGSAPDIAGKGIANPVAAILSAAMMLDHLGYGEEARIIERAVEEVLREGVRTPDLGGSATTEEVAEAIAERVATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW30</accession>
    <name>Q8TW30_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02420">
        <property type="protein sequence ID" value="AAM02420"/>
        <property type="gene ID" value="MK1207"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1207"/>
    <dbReference type="HOGENOM" id="CLU_1674013_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020948">
        <property type="entry name" value="P_starv_induced_PsiE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06146">
        <property type="entry name" value="PsiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="18579" checksum="2E54565400CF5775" modified="2002-06-01" version="1">MSERLNRFAYKFLDITVHIVMISILAITLFIFGVGVYDIVSTFLTSPYQVRFRMTFGLIVECVFDVLLLLEVYQSVLETLRHRRVPLRYVVDITIIMILRETFLKYYRGTIRPMEMLSVTAMLTVLIASRVVVLKYSPDYFKTHLERIRGQEREVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW36</accession>
    <name>Q8TW36_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sec-independent protein secretion pathway component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tatA</name>
        <name evidence="7" type="ordered locus">MK1201</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02414">
        <property type="protein sequence ID" value="AAM02414"/>
        <property type="gene ID" value="MK1201"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1201"/>
    <dbReference type="HOGENOM" id="CLU_086034_6_2_2"/>
    <dbReference type="OMA" id="RVEWELE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006312">
        <property type="entry name" value="TatA/E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01411">
        <property type="entry name" value="tatAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="66" mass="7648" checksum="9FA492D75E381681" modified="2002-06-01" version="1">MIDGLGTSELLLILAVILLLFGPKKLPELARAIGEAVGEYRRAQRRVEWELEAEERRKEKEKRDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW85</accession>
    <name>Q8TW85_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02364">
        <property type="protein sequence ID" value="AAM02364"/>
        <property type="gene ID" value="MK1151"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1151"/>
    <dbReference type="HOGENOM" id="CLU_1280808_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="215" mass="23545" checksum="729B96577326B819" modified="2002-06-01" version="1">MGHSVVLALSIIFVVALCPCHAGSWSVDPEIVEHPWLWNRYVVSKYHDPVERSKVVGLREELLDYRKARVTVVFLCTGVWVIGIALLLVKNRRAPKEALTEHFFRLASPLGFAGLAIVIASIGEFNALLALSKGEGYRVAVETIRAYGVYGPAVIALLAIAVILVTVSRRAPKVDLPFLPKTGLPLSRVSAIIAWTSVLLTSALIISYTVELFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW97</accession>
    <name>Q8TW97_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02352">
        <property type="protein sequence ID" value="AAM02352"/>
        <property type="gene ID" value="MK1139"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1139"/>
    <dbReference type="HOGENOM" id="CLU_2985690_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="57" mass="6491" checksum="25A2F3AD8F818670" modified="2002-06-01" version="1">MCEHAKALDVLGVAYTECRYGAWEPSEETLEWAIGTVEELFELLERVERDHGPASRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB2</accession>
    <name>Q8TWB2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02336">
        <property type="protein sequence ID" value="AAM02336"/>
        <property type="gene ID" value="MK1123"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1123"/>
    <dbReference type="HOGENOM" id="CLU_173284_2_0_2"/>
    <dbReference type="OMA" id="HIAIYIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025698">
        <property type="entry name" value="2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13239">
        <property type="entry name" value="2TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="2TM" evidence="2">
        <location>
            <begin position="22"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="94" mass="11049" checksum="24C6D35C2C786D0A" modified="2002-06-01" version="1">MSSETSELDEYRETFRELYYEEALKGFRAHLTIYLIGCVAMVVMNLTLTPRVIWVIWPVLGWGAGVYCHYLGVRRAERNIRRLEEEALRRVQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC3</accession>
    <name>Q8TWC3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-8 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02325">
        <property type="protein sequence ID" value="AAM02325"/>
        <property type="gene ID" value="MK1112"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1112"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1167"/>
    <dbReference type="HOGENOM" id="CLU_534874_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="423"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="509" mass="58195" checksum="F7D8A21F7A734AED" modified="2002-06-01" version="1">MLLSLVLIGILPLPPSASAATVYVHVVPYLDSDAWSQDHVAALKEFLSWCEERGIKVAVFHYNVCEETAPGITDLIREYAERGTISLVGLHSTSHALGGLPLSLQYWELEFNLQFLRDRVGSNVAQVLSVPSWVFDANTLYACDKLGVRVIVCGTDITAINPDYDPTWDRLDPIARHMLVGYFNVRGDRLYYIPALSYVSDLVQAAEDRGTTVRELLQNAVRNLIDSGVINVTGDVHLAPWVLIHPWELADADVRQEFENFLSEVGNGSFDFEYQGVTVTFELSDPREVVNMISSGGTNLGLVALPEPDYSSILHAADYHHWWELIDLYPDKSKIVREWLDTMSRWRALDSALWDLTVNGVIDDSIRKDLYRAVERVWNEALLENVLEYWDPSSARWSLLEERKALSTVYREILTLHAELVGSMVQQSRVESLEREINRLEQGLEDLSRRQQDLERRLESLEREFQGLKKQKSKRGLPVLPAPLWWKRRNPFGRRHRCSREGPSHPFCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH6</accession>
    <name>Q8TWH6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1058"/>
    <dbReference type="EnsemblBacteria" id="AAM02271">
        <property type="protein sequence ID" value="AAM02271"/>
        <property type="gene ID" value="MK1058"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1058"/>
    <dbReference type="HOGENOM" id="CLU_862241_0_0_2"/>
    <dbReference type="OMA" id="CIYCELG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="98"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="322" mass="35171" checksum="DCC95C7EDEA4E200" modified="2002-06-01" version="1">MIPTLTGRTDDPDRLMRGLEELGWTVREKGDRLVAISPGGQQVEANRETGELRITGRGEGTAARTLVKLAVKLGAEVELEGLSSEDRRPLVYGPVPSRRLGTSLGIDLPRGMCTHDCEYCSVGVSRRVSPHERFTVDPVAVREELSETLRRCDPDAVTFAGVGEPTLCANLREIAEEIRPLVEGVGAEVVLLTNSTWVSECADVVDVAVASLDCAREDLYRTINRPHPDMSLEHLVEELSQCDPGDVVVEVLLCRVGKITNADPDHLRELADLLGSIGLERVQLNTVARPPARGRAEPVGRDELLVARRTLESCGLEVSVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH8</accession>
    <name>Q8TWH8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted exporter of the RND superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1056</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02269">
        <property type="protein sequence ID" value="AAM02269"/>
        <property type="gene ID" value="MK1056"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1056"/>
    <dbReference type="HOGENOM" id="CLU_005108_0_0_2"/>
    <dbReference type="OMA" id="LHFVSHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004767">
        <property type="entry name" value="SecD/SecF-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00921">
        <property type="entry name" value="2A067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="608"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="684"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="213"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="7">
        <location>
            <begin position="583"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="716" mass="78608" checksum="FAAE0EEF94A8800E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK5</accession>
    <name>Q8TWK5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ADP-ribose pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1028</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1028"/>
    <dbReference type="EnsemblBacteria" id="AAM02241">
        <property type="protein sequence ID" value="AAM02241"/>
        <property type="gene ID" value="MK1028"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1028"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1079"/>
    <dbReference type="HOGENOM" id="CLU_037162_20_3_2"/>
    <dbReference type="OMA" id="NRDPRGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="154" mass="16588" checksum="DC1DC60787A09B87" modified="2002-06-01" version="1">MWGCSKGLSESEIRCPCICNGKYPAPALTVDGIVPYRGGIVLIRRGKKPFKGKLALPGGFVECGETVEEAVAREVREETGLKVRPVELVGVYSDPGRDPRGHVVSVCFRCEVVGGELRAGSDAADVKVVDPSDLTPDDLAFDHYDMLRDAGIVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN3</accession>
    <name>DNLI_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lig</name>
        <name type="ordered locus">MK0999</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWN3"/>
    <dbReference type="SMR" id="Q8TWN3"/>
    <dbReference type="STRING" id="190192.MK0999"/>
    <dbReference type="PRIDE" id="Q8TWN3"/>
    <dbReference type="EnsemblBacteria" id="AAM02212">
        <property type="protein sequence ID" value="AAM02212"/>
        <property type="gene ID" value="MK0999"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0999"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1047"/>
    <dbReference type="HOGENOM" id="CLU_005138_6_0_2"/>
    <dbReference type="OMA" id="WLFEESY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="SSF117018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059605" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <sequence length="559" mass="63337" checksum="8C810526CC76402A" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS2</accession>
    <name>GATB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">MK0960</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWS2"/>
    <dbReference type="SMR" id="Q8TWS2"/>
    <dbReference type="STRING" id="190192.MK0960"/>
    <dbReference type="EnsemblBacteria" id="AAM02173">
        <property type="protein sequence ID" value="AAM02173"/>
        <property type="gene ID" value="MK0960"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0960"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1005"/>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_2"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148872" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="461" mass="53159" checksum="2A5FFBE0E861506A" modified="2002-06-01" version="1">MADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIALEIAHMLDCEPVLDRPLYFQRKHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDPGRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRRLMQVLRYSGKVKGDGGIRVDANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAMRSKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALVTEREWADFFEEVVEKAPDDWDIEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLELVREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDEDRLARVVEEVIEENPQAVEDYKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-23" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS9</accession>
    <name>DGGGP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">DGGGP synthase</shortName>
            <shortName evidence="1">DGGGPS</shortName>
            <ecNumber evidence="1">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0953</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWS9"/>
    <dbReference type="SMR" id="Q8TWS9"/>
    <dbReference type="STRING" id="190192.MK0953"/>
    <dbReference type="EnsemblBacteria" id="AAM02166">
        <property type="protein sequence ID" value="AAM02166"/>
        <property type="gene ID" value="MK0953"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0953"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.998"/>
    <dbReference type="HOGENOM" id="CLU_073311_1_1_2"/>
    <dbReference type="OMA" id="DYFDYEI"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000350707" description="Digeranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <sequence length="271" mass="28174" checksum="EC2C31F90B7AE2C7" modified="2002-06-01" version="1">MRAYLELARPINCAMAALGVVVGELIAGARLDVGAVLAPVVAAVVCAGGNAINDYFDAEVDAVNRPDRPIPSGRVSPRSARMFALGCFAVGVGMATVINRMCLAIAALNSVLLYLYSWRLKGTPLIGNVMVSYLVGSCFLFGAAVGQRPAPAVWLFLLAFLANLVREILKDLEDVEGDAALGLKTLPIAYGEGVALRVATVFAIALAVLTPLPYLDGVVGWPYLVLALPAAAVILLASVLAVAGSWDAGKAQRVVKVGMLLGLLAFLASLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2013-05-29" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU6</accession>
    <name>CDAT8_METKA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA(cytosine(8)) deaminase</fullName>
            <ecNumber>3.5.4.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytidine deaminase acting on tRNA base C8</fullName>
            <shortName>CDAT8</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0935</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Science" volume="324" first="657" last="659">
            <title>A cytidine deaminase edits C to U in transfer RNAs in Archaea.</title>
            <authorList>
                <person name="Randau L."/>
                <person name="Stanley B.J."/>
                <person name="Kohlway A."/>
                <person name="Mechta S."/>
                <person name="Xiong Y."/>
                <person name="Soll D."/>
            </authorList>
            <dbReference type="PubMed" id="19407206"/>
            <dbReference type="DOI" id="10.1126/science.1170123"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the deamimation of cytosine to uracil at position 8 of tRNA in 30 different tRNAs. This editing guarantees the proper folding and functionality of the tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(8) in tRNA + H(+) + H2O = NH4(+) + uridine(8) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:50964"/>
            <dbReference type="Rhea" id="RHEA-COMP:12853"/>
            <dbReference type="Rhea" id="RHEA-COMP:12854"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="3.5.4.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-26895112">
            <id>Q8TWU6</id>
        </interactant>
        <interactant intactId="EBI-26895112">
            <id>Q8TWU6</id>
            <label>MK0935</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.35"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3G8Q">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D=1-278"/>
    </dbReference>
    <dbReference type="PDBsum" id="3G8Q"/>
    <dbReference type="AlphaFoldDB" id="Q8TWU6"/>
    <dbReference type="SMR" id="Q8TWU6"/>
    <dbReference type="STRING" id="190192.MK0935"/>
    <dbReference type="EnsemblBacteria" id="AAM02148">
        <property type="protein sequence ID" value="AAM02148"/>
        <property type="gene ID" value="MK0935"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0935"/>
    <dbReference type="HOGENOM" id="CLU_999699_0_0_2"/>
    <dbReference type="BRENDA" id="3.5.4.35">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q8TWU6"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000422595" description="tRNA(cytosine(8)) deaminase">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="175"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="2"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="33"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="59"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="81"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="92"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="102"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="151"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="164"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="259"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="270"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19407206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3G8Q"/>
        </source>
    </evidence>
    <sequence length="278" mass="30618" checksum="6F04884057684B4F" modified="2002-06-01" version="1">MKVSLAGQTVDVKKILNEIPKRTVTAALLEGGEIVAVEEADDEHAERKLVRRHDVEGKVVFVTARPCLYCARELAEAGVAGVVYLGRGRGLGPYYLARSGVEVVEVHPDEPLGYDPVDRLDVLLTFGGNPYLTEEDVAARVYCLLTGRGFDADIAPAPENLSGRVEIMVTRGDPDEAVELLKEELPVFRIRRFLISGEFDRDELRERILEDIEPRILDPFAVRARIARAGAFSSSREAEVFIGDVLTSVGREVNLNDPRTVVTVDVLGPRVSVGVEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW4</accession>
    <name>Q8TWW4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis translation initiation factor eIF2B subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0917"/>
    <dbReference type="EnsemblBacteria" id="AAM02130">
        <property type="protein sequence ID" value="AAM02130"/>
        <property type="gene ID" value="MK0917"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0917"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.958"/>
    <dbReference type="HOGENOM" id="CLU_029499_0_2_2"/>
    <dbReference type="OMA" id="PFLTHQL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transferase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="39435" checksum="2A3C2CEDD6C2D6B1" modified="2002-06-01" version="1">MNVDAVVLAGGFGTRLRPLTWDTPKPLVPILGKPLIEWVIRSLPRDVVHVHIAAGFSSEKLERYVESDPLPRKLHLKVEPKPLDTAGAIKFACRDSTADAFVAFNGDIVSSLDVRQMLKFHREHDGIATIALYPVPEDEVSRFGVVDLDDDDRILDFVEKPEPEEAPSNLINAGAYVLDREVLDYIPEGRPVSIEREIFPKLAEEGLLYGFKFEGYWVDVGLPETYLEAHRVLMEHECSGKSVVGARITDTDLKPPVVAAPMTELRSSEIGPYVYVGERTEINGSVIENSVILDDVEIIDSEVRNTIVAEGCKIENARLDGCVLGHDAEVCGCNLKGVRVAPGKEAERDLEDEWVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW6</accession>
    <name>Q8TWW6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0915</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02128">
        <property type="protein sequence ID" value="AAM02128"/>
        <property type="gene ID" value="MK0915"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0915"/>
    <dbReference type="HOGENOM" id="CLU_1458202_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="185" mass="20626" checksum="103DE47A293D01CB" modified="2002-06-01" version="1">MIATLALVNAYAASIPRYQIVKGKVTYPYFDEHDLLKTLQYPSVLGPAINTYLPTRTVVNCRQIMLYGLKRVGVISIGAPVTMLPTLNYDNLTKSSDNPIVTYYGISSNPICLSFPCIDVVKIEKDLLLIPTRNSLVVAKGRGEVIKGIEVVLIQGNWNIICMLPENAYSGVFTWKSELQPFWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ2</accession>
    <name>Q8TWZ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted enzyme related to selenophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0889"/>
    <dbReference type="EnsemblBacteria" id="AAM02102">
        <property type="protein sequence ID" value="AAM02102"/>
        <property type="gene ID" value="MK0889"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0889"/>
    <dbReference type="HOGENOM" id="CLU_948680_0_0_2"/>
    <dbReference type="OMA" id="MEGPYPL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011414">
        <property type="entry name" value="UCP036541_AIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036541">
        <property type="entry name" value="UCP036541_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="51"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="187"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="301" mass="33097" checksum="55AEF5E04FCD8442" modified="2002-06-01" version="1">MSLEKVDLERLLEHIEETNFCRRLFREVSEEWKTIIGPLQGDDATVIEVNGERLVINMEGPYPAKIGRKTAIIHSAADVVVTGGEPIVAFDAVQAESEEQAEEILEDLRKQAEGLGIKILGGNTQSHPDLVPCVSVVVIGRLIADEPIPDGTAREGDALVFLGEPVRGDVGDRVYKAKVKFNAFLRFLREGIDVSAAKDASRGGVLGNLLEMMGKAKKGVELRSMPYPTWTGYLGIFMVCMDPNDVERAAEIAFEEGCPFTVAGEVVDEPVIRFGNRELVSEEEMIEVYRRLPYKPPGAGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-05-16" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ3</accession>
    <name>TRUA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">MK0888</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWZ3"/>
    <dbReference type="SMR" id="Q8TWZ3"/>
    <dbReference type="STRING" id="190192.MK0888"/>
    <dbReference type="EnsemblBacteria" id="AAM02101">
        <property type="protein sequence ID" value="AAM02101"/>
        <property type="gene ID" value="MK0888"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0888"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.930"/>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="OMA" id="MYIFKIA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000057504" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="256" mass="29254" checksum="136C190E515968A4" modified="2002-06-01" version="1">MRVALRVAYDGSRYHGFQYQPDVPTIEGALRKALSELGLELVGYASRTDAGAHARYQVVVVEGDPELAQPDPINARLPKDIRVIAKTEVHEEFDPRRDALRKEYRYFLGPLNRPEAAARAARKLEGKHDFSAFRREDGRNPIITVERCELVEITPNAYVLRVVAPRFLWEMVRRIAGFVWEVGHGLREEGDAEALLSGKFEPSKKPRCLPAEGLILWHIEYDEVRFERTKAWFEDHKVIQLGGRLLLRLPEGAESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX45</accession>
    <name>Q8TX45_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized membrane protein specific for M.kandleri, MK-24 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02044">
        <property type="protein sequence ID" value="AAM02044"/>
        <property type="gene ID" value="MK0831"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0831"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.874"/>
    <dbReference type="HOGENOM" id="CLU_827976_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="34872" checksum="52FB5EB875D47AF2" modified="2002-06-01" version="1">MKRVLLALAVALAVALVGGAEAKVYSGKYTIKPQIQSVEAPSEVQLEKGQTTADVTVTATITWTGNYPYTIKRTPTTVTFAVDGQTVDTKTINPPYKLGDTRTVSTTLKLTEGKHTITVTAKFPDLPQYGITGGTATQSVEVTVVAPKPAIPTHLAGAVVKDLLLAVPALMLLDPFMGYPLSDMLGYKTDLQNFLDDLGLREFAENDVGAVLPAMVLATDLLAGWGLDHNVMPNTLSMLDVGTMAGAVVAPLLYALGVGDVENPLVDLAQELGLEGSAVITEASAVGLALPTVSTVLGYLADKGVATAAQIVAPVVKALDQLLTMLASPIAGYLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-25" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX51</accession>
    <name>RL1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl1</name>
        <name type="ordered locus">MK0825</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX51"/>
    <dbReference type="SMR" id="Q8TX51"/>
    <dbReference type="STRING" id="190192.MK0825"/>
    <dbReference type="EnsemblBacteria" id="AAM02038">
        <property type="protein sequence ID" value="AAM02038"/>
        <property type="gene ID" value="MK0825"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0825"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.867"/>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_L1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_L1_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125799" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="214" mass="24111" checksum="44C7AD95B47AEF9D" modified="2002-06-01" version="1">MTITEEDLIEPLRKVVEYSPPRRFLETVDMIVNVKGVDLSDPSQRIDKEVVLPHGRGKPVNVCVIAEGEMAREAEEAGATVINREKLEELAENVREAKKIARRHEFFYAQVDLMPDVGRVLGPVLGPRGKMAKPVPPNADIRALIERAHRTARVRMRDQPVIHTVIGARNMEPEQLAENAMAVLREITSELEKSWAQIDSVYVKTTMGPAERVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX70</accession>
    <name>Q8TX70_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02019">
        <property type="protein sequence ID" value="AAM02019"/>
        <property type="gene ID" value="MK0805"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0805"/>
    <dbReference type="HOGENOM" id="CLU_1060114_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="262" mass="29832" checksum="BA6679B12BF037B7" modified="2002-06-01" version="1">MDPHETMRRALREGGVHRVRMSEPGRRLVVDSVGPYLRYPIVTAFVARRDDGAIVELVAFRDESVDVIETALAVEHREKGRDILVDANLKPLEDMYGVEADKEEVDRVVYAKVHPELHRLLGSEWLSVRFFGPTVGLGSGDVPTLVTRGLGAHIVSELLGEEPAFIDDGEKTHRPYQEVPPERVEWVLAELTARGYGVVFTKPAVEVLYTLCREGYASPDRVEWVYRTQRSWCERPESWPRRSIAERNLEALEYGLELLRDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-11-28" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX89</accession>
    <name>MPTD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name type="ordered locus">MK0786</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2009-02" db="PDB data bank">
            <title>Crystal Structure of uncharacterized conserved archaeal protein from Methanopyrus kandleri.</title>
            <authorList>
                <consortium name="New York structural genomix research consortium (NYSGXRC)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.33 ANGSTROMS) OF 2-121</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="2IEC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.33 A"/>
        <property type="chains" value="A/B/C/D=2-121"/>
    </dbReference>
    <dbReference type="PDBsum" id="2IEC"/>
    <dbReference type="AlphaFoldDB" id="Q8TX89"/>
    <dbReference type="SMR" id="Q8TX89"/>
    <dbReference type="STRING" id="190192.MK0786"/>
    <dbReference type="DNASU" id="1476887"/>
    <dbReference type="EnsemblBacteria" id="AAM02000">
        <property type="protein sequence ID" value="AAM02000"/>
        <property type="gene ID" value="MK0786"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0786"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.828"/>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="OMA" id="FHQFVGT"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="EvolutionaryTrace" id="Q8TX89"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="SSF143560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000420353" description="Dihydroneopterin aldolase">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="55"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="104"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source ref="2"/>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2IEC"/>
        </source>
    </evidence>
    <sequence length="121" mass="13735" checksum="944CED707FAC7743" modified="2002-06-01" version="1">MKYFKRLSDRERAIFEAGITLGAIYHQFCGTPVSPGTAEEVAKCIERAALLQPCVIDARVEVDVSSEDTDNYGGYTEVSGRNLRVTIVTRCGEWEAVGKLEFIEELNYPLMWVEEIRRVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX90</accession>
    <name>Q8TX90_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing V4R domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01999">
        <property type="protein sequence ID" value="AAM01999"/>
        <property type="gene ID" value="MK0785"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0785"/>
    <dbReference type="HOGENOM" id="CLU_1922806_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="68"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="131" mass="15005" checksum="81F4DD2D007B1AC0" modified="2002-06-01" version="1">MDELIKDLEEGAGEKGELLAEIVYERADSLEEMLELLEDMGMEGIKVDFVGDKEDPEEIVITVETTVTVEFYEENPEVKEEMGFTEPACELEQALFRKLAELKYDKSMEIEETKCQLAGDDICKFRIVPKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX91</accession>
    <name>TRPB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpB</name>
        <name type="ordered locus">MK0784</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX91"/>
    <dbReference type="SMR" id="Q8TX91"/>
    <dbReference type="STRING" id="190192.MK0784"/>
    <dbReference type="EnsemblBacteria" id="AAM01998">
        <property type="protein sequence ID" value="AAM01998"/>
        <property type="gene ID" value="MK0784"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0784"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.826"/>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000099039" description="Tryptophan synthase beta chain">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <sequence length="398" mass="43756" checksum="476AD46D43C4876C" modified="2002-06-01" version="1">MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTPLYYARNLSRKLGVKVYLKREDLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLATAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHPVKAGTQTLKDAINEALRDWITNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSIGIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTHSIAPGLDYPGVGPELAFLVDEGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYVKKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE1</accession>
    <name>Q8TXE1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0733"/>
    <dbReference type="EnsemblBacteria" id="AAM01947">
        <property type="protein sequence ID" value="AAM01947"/>
        <property type="gene ID" value="MK0733"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0733"/>
    <dbReference type="HOGENOM" id="CLU_081191_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PTHR42282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="2">
        <location>
            <begin position="80"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="3">
        <location>
            <begin position="195"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="286" mass="31202" checksum="DA21846CFE221D19" modified="2002-06-01" version="1">MKLSVPGALTGFFHPQPGKTPKDTGSPGFGLAVDSVVIVRVRRLRAERLRVRADHPIDSRIARKCYEVLDPDGGLSVRYEFSVPPGCGLGTSAASALGTLAAGAAEIGLEVSPEWIARRAYEIELELGTGIGDVVTIWHGGAVLRVGPGYPDEVLIHRIPVEPDLRVLIYEPRPIETREALDGLSGTEVALRYLKELERHPDLELALRRSLEFAERLGFSEEVKLAKELSHKHLGASIAMLSRTVFAVSWEQDPQADVELPVSCSSFPEIISGISEIQCERGLRDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE2</accession>
    <name>Q8TXE2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="3">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="3">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 8" type="primary">sucD</name>
        <name evidence="8" type="ordered locus">MK0732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0732"/>
    <dbReference type="EnsemblBacteria" id="AAM01946">
        <property type="protein sequence ID" value="AAM01946"/>
        <property type="gene ID" value="MK0732"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0732"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.772"/>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="3">
        <location>
            <begin position="93"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="3 4">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="287" mass="30872" checksum="7798944654C77D29" modified="2002-06-01" version="1">MLDSDTRVLVQGITGRHGSFHTKLMLEYGTEIVAGVTPGRGGQEVHGVPVYDTVEEAVEETDAEASVIFVPAPQAPDAVMEAIEAGLDPIVVITEHIPAHDTMRFVELARWEGIRIIGPNTPGIIVPPERVKLGIMPHQVFTEGEVAVASRSGTLTYEIVQAMTEAGLGQSLCVGLGGDQIVGTTFIDFLEYVREDDRTEAVVLIGEIGGNAEELAAEYIAETDFPKPVVAYIAGRHAPPGKRMGHAGAIIERGRGTAESKIEAFRKAGVDVAEKPWEVPELLREYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH7</accession>
    <name>Q8TXH7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0697</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01911">
        <property type="protein sequence ID" value="AAM01911"/>
        <property type="gene ID" value="MK0697"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0697"/>
    <dbReference type="HOGENOM" id="CLU_332241_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="861" mass="98093" checksum="C3FC22A0E063F019" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK1</accession>
    <name>Q8TXK1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0671</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01886">
        <property type="protein sequence ID" value="AAM01886"/>
        <property type="gene ID" value="MK0671"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0671"/>
    <dbReference type="HOGENOM" id="CLU_2565765_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="81" mass="9528" checksum="A3273CB8B9D95545" modified="2002-06-01" version="1">MQFNRVRLEGEREELEIRIGSANVKRKLAMLIREGDLVLEERRELEPHEEVEVLAGYEEPEEGVPTERLKVLRVKRVEFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM5</accession>
    <name>Q8TXM5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0637"/>
    <dbReference type="EnsemblBacteria" id="AAM01852">
        <property type="protein sequence ID" value="AAM01852"/>
        <property type="gene ID" value="MK0637"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0637"/>
    <dbReference type="HOGENOM" id="CLU_1227659_0_0_2"/>
    <dbReference type="OMA" id="LISAYHY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="51"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="225" mass="25416" checksum="F2A61074B6D0DF09" modified="2002-06-01" version="1">MIEKEPVPLQVGDYRLYFRLWALGDADAISDVLYRELYPHEWLEGSREVVDVGAHVGAFTVLAAAHGAREILAIEPHPDNAELLRRNVEENDLNAEVVEAAAYDREDVVKMYLSPSTVAHSVELVRSRETIDVETVALDDLGTSPDLIKIDAEGAEERVLRGAERTLEEHAPVLLISAYHYPGQEEDLRRWLEDRNYRVEVLVRETSPYRSPALRVPVIVAEPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM9</accession>
    <name>Q8TXM9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0633"/>
    <dbReference type="EnsemblBacteria" id="AAM01848">
        <property type="protein sequence ID" value="AAM01848"/>
        <property type="gene ID" value="MK0633"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0633"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.673"/>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="2">
        <location>
            <begin position="25"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="383" mass="42404" checksum="C86ADF6273BC1F18" modified="2002-06-01" version="1">MEKLVLLPGPVMVHEDVLLRSAKQVMNHRSEEFEEILTECVELSKYLFQTDGNVAIITGSGTAAMEAAIYSLLEPGEEVVAVVNGKFGERFADIAERRGAEVRRVEFEWGKVADPERVEEALADSDAEVVTLVHNETSTTVLNPAEEIARICREYDALFVVDAISSLGGVEFRMDDWGVDVCVTGSQKVLGCPPGLALVAVNDRALEVMEEKDAGSYYLDIPKYLEYLEKDPKQTPYTPAVNAFYALHEALKRLKEEGLEARWERYRLMQRIVREGIRALGLELFVEDDEIASPTVTGVTYPEGVDDREFRGEILRDRYDVVVAGGQDHLAGEIFRIGHMGEVRIRDMITAVTCIGLGMRELGVDVDVGAAAEAMADVLSEVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-27" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP3</accession>
    <name>DNAG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name type="ordered locus">MK0617</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Also part of the exosome, which is a complex involved in RNA degradation. Acts as a poly(A)-binding protein that enhances the interaction between heteromeric, adenine-rich transcripts and the exosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA. Component of the archaeal exosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXP3"/>
    <dbReference type="SMR" id="Q8TXP3"/>
    <dbReference type="STRING" id="190192.MK0617"/>
    <dbReference type="EnsemblBacteria" id="AAM01832">
        <property type="protein sequence ID" value="AAM01832"/>
        <property type="gene ID" value="MK0617"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0617"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.654"/>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="OMA" id="KYMIVAQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000240457" description="DNA primase DnaG">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="175"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="409" mass="45908" checksum="44FB591B225E30B9" modified="2002-06-01" version="1">MSKGRTDYGNGAVKYVIRAKLRANGYVERPDVVGAIFGQTEGLLGDDLDLRRLLKTGRIGRIDVKLRNENGKTVGEIIVPSSLDRVETALVAAALEQVDRIGPCRAEVEVVSIDDIRKEKRERMIRRAREILREMVSEVTPDSSELVQKVKEAVEDVEVEEYKGLPAGPNVEDSDAIIVVEGRADVANLLRCGIKNVIAVEGTNVPEAIVELSKEKTVTAFVDGDRGGELILKELLQVADVDYVAKAPKGKEVEELTRKEIKRALERKVPVEEYLKEIGERPKDKEREKGKKPKPKKRPERRGRPRKKKARPKRGPQERRLLDRLKRLKGTFRAEFLDEGLKPVKEVELDELVEKLKSEDGVRAVVLDGVITRRLVEAAREKGVKYVVGVKEGDLDPEIKKDVKIITMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR8</accession>
    <name>Q8TXR8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri with repeats, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0592</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01807">
        <property type="protein sequence ID" value="AAM01807"/>
        <property type="gene ID" value="MK0592"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0592"/>
    <dbReference type="HOGENOM" id="CLU_1096717_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="253" mass="26779" checksum="E086879664A0AE1F" modified="2002-06-01" version="1">MEVTIQLSGVVEGQQVTDVYPVTITVQLVKLNSSVNITVEKITESEAVLRVDYQINTENCSVKSVEVKLLKDGNVVASEERSQAEGTVRFTVNESGTYRVVIEATVEWTTPSGRHEEKLTLASEDVEVTLSRLEVGAPSVKVSDYGADWATLEITVPVTAVGCEVTGGKVEVIEAGTGHTLATTEATVDNGVLKATVKLTGLRSARIPIIVRVTAEGKTPTGDSVTATAEASVEVRLASAELGGSVRVESPRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS4</accession>
    <name>PRIS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priS</name>
        <name type="synonym">pri1</name>
        <name type="synonym">priA</name>
        <name type="ordered locus">MK0586</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01801" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01801.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXS4"/>
    <dbReference type="SMR" id="Q8TXS4"/>
    <dbReference type="EnsemblBacteria" id="AAM01801">
        <property type="protein sequence ID" value="AAM01801"/>
        <property type="gene ID" value="MK0586"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0586"/>
    <dbReference type="HOGENOM" id="CLU_878822_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000046743" description="DNA primase small subunit PriS">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="296" mass="33662" checksum="7619D544064CFC4A" modified="2003-08-29" version="2">MDVKTLVGEYYASLPSGVLREFVEDPSSREWAYTFLKGRDIVFTRKRRLDEVSGFLELYHSTGKFKNPKSWEGLLGWDLVIDVDAELPEEPEAFLKSLGRLLKDVVIACDELRRALGFPRPDVVNFSGSKGFHVRYFDSTVRRWLRWDLHERRGIKPGEIIQRVGRGVVWLAREGFVAGDRVRALREGLLDDSMYDLKRLIRCVGSMNVKSLLPAVPVWSREGGRWVDFRDEVLGMNDLELGCLVAHRTLTWPGRGGLGVVLSRVLDLDTDADPEDPSDFVEVWGNVMSVLGELKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV1</accession>
    <name>Q8TXV1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-hydroxybenzoate + 5-phospho-alpha-D-ribose 1-diphosphate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0558"/>
    <dbReference type="EnsemblBacteria" id="AAM01773">
        <property type="protein sequence ID" value="AAM01773"/>
        <property type="gene ID" value="MK0558"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0558"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.593"/>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="OMA" id="RLNGAWY"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="PTHR20861:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_C" evidence="3">
        <location>
            <begin position="225"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="328" mass="35040" checksum="0C944C1C9803C6A6" modified="2002-06-01" version="1">MVRVRSVSRIHVTLIDLHGGLGRVDGSVGVTLEGPRIELEVEPTGEGVKVDGEGEIAEKAERAARKVLDLYGIEGGVRIEVVRRYPEHVGLGSGTQATLSAAVGTLEAHGVEHYDVRELADALGRGGTSGIGVAAFERGGFIVDGGHVFGPGGKEEFKPSAASGEVPPAPVISRLEVPEDWRFVLAIPEVERGAHGDKEVNIFKRYCPVPAREVGEICRWILMVMMPAVVEDDPEDFGRAVDAIQDLGFKRVEVGLQHPVVREMMEVARSAGAYGAGLSSFGPTVYAVCDSPSARDVAQELEMYMREEGIGGEVSVSEPRNEGFEVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ4</accession>
    <name>Q8TXZ4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rIO1_1</name>
        <name evidence="12" type="ordered locus">MK0514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0514"/>
    <dbReference type="EnsemblBacteria" id="AAM01729">
        <property type="protein sequence ID" value="AAM01729"/>
        <property type="gene ID" value="MK0514"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0514"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.546"/>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="OMA" id="EIRCAKV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="57"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="263" mass="30823" checksum="D38FFAC9141BCF88" modified="2002-06-01" version="1">MTLSEDKYFSAIEELEPKPGKVKRLEKTLEDLKVEEEVFDQYTLMTLYELSRRGYIDGLMGFVKTGKEANVARGVRGDELVAVKIYRAATSDFRRMWRYIRGDPRFKNIGRKRHRIVYAWTEKEFKNLTRAYEAGVRCPEPIAHMNNVLIMEFIGDDEGNPYPLMKDNPPEEEYAEDVLSNILDDYRRMYRKAEIVHGDLSEYNILYGGKGDYRIIDFSQGVVLDHPIAEELLIRDLRNLIRFFERLSVDTPTLEEALEYVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-10-07" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY01</accession>
    <name>Q8TY01_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histone H3/H4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hht1_1</name>
        <name evidence="6" type="ordered locus">MK0506</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01721">
        <property type="protein sequence ID" value="AAM01721"/>
        <property type="gene ID" value="MK0506"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0506"/>
    <dbReference type="HOGENOM" id="CLU_2629742_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="5">
        <location>
            <begin position="4"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="77" mass="8624" checksum="F11051B025073A38" modified="2002-06-01" version="1">MRKKLPVAPFDRALRAVGEDVRVSRKASETLRDHVQRLAEEIGKRAGEIAASRGSRFVERVDVERAFAEVVFGIDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY12</accession>
    <name>Q8TY12_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted HD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0494"/>
    <dbReference type="EnsemblBacteria" id="AAM01709">
        <property type="protein sequence ID" value="AAM01709"/>
        <property type="gene ID" value="MK0494"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0494"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.524"/>
    <dbReference type="HOGENOM" id="CLU_073842_1_0_2"/>
    <dbReference type="OMA" id="GRSKTHG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004454">
        <property type="entry name" value="HD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00295">
        <property type="entry name" value="TIGR00295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="17"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="19417" checksum="CBD9CCC50ACAFA2E" modified="2002-06-01" version="1">MRDPIRDLERVGCPEHVIEHCKAVCRLAEEMAERCEEDVDLNLVRTGALLHDIGRARTHGIDHAVVGANIVSELGYPKEVVRIVERHIGAGIPKDEAKKLGLPPKDYIPETLEEKIVAHADNLTFGTEHVPIKVVVRKFSERLGEDSPAVKRLIELHNELVDLNAIPPELTRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY49</accession>
    <name>Q8TY49_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcription regulator containing the wHTH DNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0457"/>
    <dbReference type="EnsemblBacteria" id="AAM01672">
        <property type="protein sequence ID" value="AAM01672"/>
        <property type="gene ID" value="MK0457"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0457"/>
    <dbReference type="HOGENOM" id="CLU_158394_0_0_2"/>
    <dbReference type="OMA" id="SGPFGGY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_11" evidence="1">
        <location>
            <begin position="70"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="145" mass="16629" checksum="8D099F32BDC416D2" modified="2002-06-01" version="1">MARDDEIAVLTQSRLERVARSRPRGDLWEFLKRAYEKGVKIDAGHLIILSVLEEANRLLDQLSKTVGEKRAKQILKEAGIYTKTGNYVSGELLKEYINRESRVAVHNRVKDLRKMGFKIDGKPGPDGGYSLIQVPEWYRKSSRED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY71</accession>
    <name>DAPF_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diaminopimelate epimerase</fullName>
            <shortName evidence="1">DAP epimerase</shortName>
            <ecNumber evidence="1">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapF</name>
        <name type="ordered locus">MK0434</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY71"/>
    <dbReference type="SMR" id="Q8TY71"/>
    <dbReference type="STRING" id="190192.MK0434"/>
    <dbReference type="EnsemblBacteria" id="AAM01649">
        <property type="protein sequence ID" value="AAM01649"/>
        <property type="gene ID" value="MK0434"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0434"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.463"/>
    <dbReference type="HOGENOM" id="CLU_053306_3_1_2"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149887" description="Diaminopimelate epimerase">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="279" mass="30994" checksum="64603E6D480EAB6D" modified="2002-06-01" version="1">MQFWKVHGARNDFVLVDETEEEVVPESDKPDFARWACDRRSGVGADGVVFIRSDPPSVEMRIFNRDGSEAEFCGNAARCVVKYVTEVRGENVKILRTLSGAHRVEVQGGWIAVEVPEAEIKKVVELGYEVDAGVPHFVRLTERDPIHDFGGLTDEAKTIFSEYEPKGGVNVTYAAPSVDELRVRTFERGVGWTPACGSGVVAASLVYSEIFGPFEEVSVRTAGGCLRVSLSDGPLLIGRAEIVYKGELRGDWRENTDHQRRRHSLSRSPSGRPRLQECR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY93</accession>
    <name>RL2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl2</name>
        <name type="ordered locus">MK0412</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY93"/>
    <dbReference type="SMR" id="Q8TY93"/>
    <dbReference type="STRING" id="190192.MK0412"/>
    <dbReference type="EnsemblBacteria" id="AAM01627">
        <property type="protein sequence ID" value="AAM01627"/>
        <property type="gene ID" value="MK0412"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0412"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.441"/>
    <dbReference type="HOGENOM" id="CLU_036235_0_3_2"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_L2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_L2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129716" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="204"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="243" mass="26415" checksum="42E713956B5C3439" modified="2002-06-01" version="1">MGKRIRPQRLGRGGPTYRAPSHRYRGRIEHRPYDEQEKKGKVVGKVVELLHDPARNAPVARVRFEDGEERLILVPEGTKVGDIIECGVSAEIKPGNTLPLAEIPEGVPIFNIEGQPGDGGKFARAPGCYATIIAHDVGRTYVQLPSGKVRTFDPRCRATIGVMSGGGKREKPFVKAGKKYYHMRSKGGKWPKVRGVAMNAVDHPFGGGNHQSPGKPTTIARGDPPGRKVGHIAARKTGRGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA6</accession>
    <name>Q8TYA6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0396"/>
    <dbReference type="EnsemblBacteria" id="AAM01611">
        <property type="protein sequence ID" value="AAM01611"/>
        <property type="gene ID" value="MK0396"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0396"/>
    <dbReference type="HOGENOM" id="CLU_896051_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006342">
        <property type="entry name" value="FkbM_mtfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05050">
        <property type="entry name" value="Methyltransf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01444">
        <property type="entry name" value="fkbM_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_21" evidence="1">
        <location>
            <begin position="89"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="310" mass="35555" checksum="A6E82B6A2E6EBFE8" modified="2002-06-01" version="1">MGDRPRRLGSRLLYAGCVLLTETVDAIMDKLKSNCDIVRVQVEDVSFHLRVHTNDAGVSRELRLRNIREPKASKYLVGKFLNDEEIAFDVGANIGYYAILTALASERSRVYAIEPVRENLELLRENIALNNLEDRVKAFEYAVSDKCGRIRMILENRSNWHRIVNAEDGDYIEVESITLDELSEKLGERPTYVRMDVEGAELEVIRGMVELLESDDPPKLFIEHHIHLLGLDATLDLIETLLDYGLEIAAAFGYPHASLHDREGGYRPLVGEVVRWRGLDVELYEPSFEELHDVIVEKSWDCFHVFYRPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC3</accession>
    <name>RL37A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L37Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37ae</name>
        <name type="ordered locus">MK0379</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC3"/>
    <dbReference type="SMR" id="Q8TYC3"/>
    <dbReference type="STRING" id="190192.MK0379"/>
    <dbReference type="PRIDE" id="Q8TYC3"/>
    <dbReference type="EnsemblBacteria" id="AAM01594">
        <property type="protein sequence ID" value="AAM01594"/>
        <property type="gene ID" value="MK0379"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0379"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.405"/>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="OMA" id="ISTGIWQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_L37Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_L37ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139844" description="50S ribosomal protein L37Ae">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <sequence length="89" mass="10216" checksum="3D0F898559775144" modified="2002-06-01" version="1">MGRTKKVGPAGRFGPRYGMRIRRRVAEIESVQRQKHECPVCHKRAVKRVGTGIWRCTKCGAEFTGGAYYPETEAQRIVRRAIRKALEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH7</accession>
    <name>Q8TYH7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0322</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0322"/>
    <dbReference type="EnsemblBacteria" id="AAM01537">
        <property type="protein sequence ID" value="AAM01537"/>
        <property type="gene ID" value="MK0322"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0322"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.343"/>
    <dbReference type="HOGENOM" id="CLU_063409_0_0_2"/>
    <dbReference type="OMA" id="CAPTMWG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="325"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="377" mass="41776" checksum="3D11D41445E630DB" modified="2002-06-01" version="1">MSSVEVQVGGCYLARSSDPDTVKEAACGGVVTELLRHALREDLVDVVVCVERGADALDGRPIVVDDPAEVPSGSYHCAPTQLARLVAELHREDPTLRVAVTCRPCDARTLDRLAERDLVNPDRVYRIGLNCGGTFEPRVVLEILEEHGVDPLDVEREEVVKGHLVIELRDGEEISVSIDELEEQDSGRRENCRRCDENIPERADLACGNWGVPDDLQGEWTFVEVKTEDGRDLLAGALDAGTMEVQEAPGKSVQIRAKIDEVMRKMARSWREKTLDEDWLTEAIEALDRCIKCRSCRQVCPVCASCEEGCWSFAGRESVTPTPVWHAHIAQCVALYCVECGACETACPAEIPLTRIYSVLRERLPDVVERLAEPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH9</accession>
    <name>Q8TYH9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted dehydrogenase related to phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">serA</name>
        <name evidence="5" type="ordered locus">MK0320</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0320"/>
    <dbReference type="EnsemblBacteria" id="AAM01535">
        <property type="protein sequence ID" value="AAM01535"/>
        <property type="gene ID" value="MK0320"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0320"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.339"/>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_2"/>
    <dbReference type="OMA" id="PHIAWAY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="16"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="111"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="27837" checksum="DAAE46F087F207F9" modified="2002-06-01" version="1">MPGRGCAHDEDPGHGPVHEDALRKLEELGEVVLEDADEEGLRRHVRDADAWVVRSGTQVTRELIEEAENLKVIARAGVGVDNIDVEAATERGIVVVNAPESSSISVAEHTMSLMLVLARRIPQADRSVRRGEWDRKLFMGVELAGKVLGVIELGRIGRQVAKRTKAFEMEVIAFDPYISEEVAEGLDVELVEDLDELLRRADVVTIYVPLTDETQGMIGERELKLMKESAFLINCARGEVVDEEALVRACSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI0</accession>
    <name>Q8TYI0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted dehydrogenase related to phosphoglycerate dehydrogenase, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01534">
        <property type="protein sequence ID" value="AAM01534"/>
        <property type="gene ID" value="MK0319"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0319"/>
    <dbReference type="HOGENOM" id="CLU_2820877_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="66" mass="7434" checksum="3B24131C3ECE9732" modified="2002-06-01" version="1">MQLLKGRLRSVVIKVGEEPGDREREALKRAMLKGCLDRILQEPVTMVNAVRVKDSHPPELVVVEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI9</accession>
    <name>Q8TYI9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01525">
        <property type="protein sequence ID" value="AAM01525"/>
        <property type="gene ID" value="MK0308"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0308"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.317"/>
    <dbReference type="HOGENOM" id="CLU_1163805_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26472" checksum="12C93F644100E4BE" modified="2002-06-01" version="1">MTEASFDGKYVKRVFGVPDEVVDGAVVRAWDIIRERRSKGRVRIRRPDEVWPIGRAVARLASVARVSVRRVKGVECPEDALDQVLGDAEPSSPALRTLRSLTRGRCTIGFPVLTPELTLVVWGANTEELSSSVLDLTHHPAVRTLVSLAVLDDVGEYLVDRYDDRAEQLSEALQRAYDGRGARLLGLCLCAWLSEKLGGEFETWWNEGGGWRALYSVVRESELVESTVRERVGVEPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ2</accession>
    <name>Q8TYJ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01522">
        <property type="protein sequence ID" value="AAM01522"/>
        <property type="gene ID" value="MK0305"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0305"/>
    <dbReference type="HOGENOM" id="CLU_800797_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="39806" checksum="65A51A5B7861B775" modified="2002-06-01" version="1">MEAPWVMDPELYQSHTVQFEGSGIPPARPSDPNAFQADRFLLVAPLWNEEGRHRVIIGAYRTETVPLPEDAKVCRVLVPDRVEVVETDNPFSDERGDPAWGILSAPRHFKKPVPTGPWHEVKYTLEEHDGILLIRPERTADDIFAAEADLPTRDGWVRIALTPGNWLVLLGWSKEFNLLTVDGKTVFRGRVPYNEVKYDLDGVSMVFWYCGYAIDLKGYKWFKDVFNSGTSRECWLMLHYIDNLVSSKSNKASTDILNFHPLLQYKIGILYGPGIILRINTRNPIIIEFKAPKPRDYIKYGYKEFYSDKYWSNSLSPLEVIVSQILYGVICPVITPPILLIHRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ9</accession>
    <name>Q8TYJ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01515">
        <property type="protein sequence ID" value="AAM01515"/>
        <property type="gene ID" value="MK0298"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0298"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="24175" checksum="216D97793079F147" modified="2002-06-01" version="1">MIESHVPIPSDPVERIRALRVLREVHRRKRKPSLEVTYRTVSGSTCGPYYVARWRRDSKFKHGRTLYLGKPENESVRFVEWLVSLDRNEVLELARHLMRNLRSVLKTLLTGVSDLPYKRARRVLARGLALTFDARPSESPRIRDVLEELPDRLESFAIRTLGGWPAHYSAHLRKVIRHRRKSLDGKHEIPDVKLELERWKLRHVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM0</accession>
    <name>Q8TYM0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted P-loop containing NTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0276"/>
    <dbReference type="EnsemblBacteria" id="AAM01493">
        <property type="protein sequence ID" value="AAM01493"/>
        <property type="gene ID" value="MK0276"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0276"/>
    <dbReference type="HOGENOM" id="CLU_1029012_0_0_2"/>
    <dbReference type="OMA" id="NPVHRLW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="30270" checksum="9511D2432D189372" modified="2002-06-01" version="1">MTHVVLVVGLLETDSGKTTAALPLVSALDLVPFKPRSGHNVWLHYDHTRRCVELGLPVSRDVLKLAKVAEVDLPLITLNPVHRVWTSPDVGTAVKSDVSLRYFSAQTDAYVLMDRLDETVFLYTEGTELRYVPDETMEMARECEVREVREEPAPEELRRTIDRAWSRVRKEGPVLVESLNNLAVPWPGVLKYDGVAVAVGPGTALVYDLRDYARAAETLAGPEAVTLSLLEVLEPLEVVRLPPLDRTRRSDPRKLEEAYRELIGAVQERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ0</accession>
    <name>Q8TYQ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01459">
        <property type="protein sequence ID" value="AAM01459"/>
        <property type="gene ID" value="MK0242"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0242"/>
    <dbReference type="HOGENOM" id="CLU_699452_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="394" mass="42232" checksum="58EDF5A12364D5CD" modified="2002-06-01" version="1">MGRFERNLAAMFTEYALISTLQALFWIVLPRTLGPQQFGKLVLLINLAGFLSDPIHQAVAFACSPELLGLGDRERASLCVSAVIVSLMLSFAVASAMLLAITFLPVPEKLRIVSDPWVASGLLVLCMSPVTKVVEPFLLAAGRNVFVVAIALGHAAKVVIPPLLRTSWVGAAICTGLCPLLAGLATLPLVGRPRGWNFAIGYLTRGMVIVVDRLVELGSYAYAVLLTYLLYGSKTSAFVGLGLAAYKFAALTLEAFVPPITLEVGAGEEEQAFRGLRRYLVPASIATAVAIVSCADLAPVVLSTEYGPAVPIVRWFSLPTLLLPYHIALRAYLIGSRRLRLLLVYRTLYASSFFVILHTLTGAFGPVSVPLGLLAALSSAPPMVAMIRRHTVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS3</accession>
    <name>Q8TYS3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing PUA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0219"/>
    <dbReference type="EnsemblBacteria" id="AAM01436">
        <property type="protein sequence ID" value="AAM01436"/>
        <property type="gene ID" value="MK0219"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0219"/>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="OMA" id="GANAYEP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015266">
        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="PTHR22798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09183">
        <property type="entry name" value="DUF1947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="83"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="166" mass="18517" checksum="D2BFF03C22BAEB71" modified="2002-06-01" version="1">MKVSRRHPISEKDLQEVLEELRVSSGVSLGEVLTGLVEVAYVKDDDIERLLIKDGKVMAFERHEGWFPTIHALLQLDEGNYGHVVIVDMGAVKPVASGADIMVPGIVEVRGEFEEGDGVVVIDERNRRPLAVGIALMSAREIEESERGRAVRNVHHVGDRLWEARF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW2</accession>
    <name>Q8TYW2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0179"/>
    <dbReference type="EnsemblBacteria" id="AAM01396">
        <property type="protein sequence ID" value="AAM01396"/>
        <property type="gene ID" value="MK0179"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0179"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.179"/>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="OMA" id="CKLNEYE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="14"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="33100" checksum="0A5F5FA78D051125" modified="2002-06-01" version="1">MGKATIATAQLSSCVGCHVSLLDLHEKLLDLLEDAIEIEYCYVLVDQKEIPEHVNVAVIEGSIRNEEDLEVAEELREAADIVVAVGTCACYGGVHGLANLYQLDDILEWVFKETPTTDDEGETPEEVVPELFGYVRPLPEVIDVDYMLPGCPPKPESIAEVITAILEDREPELPTTNLCEECPREKEDIPIEEIKFRTGQGRPDPDKCLLEQGYPCMGPATVAGCGAACPSRGLSCRGCNGPTKQALDQGAAFLDAIASVSFETDVDVEEILEGLVDLPGKLYMFSMAASLLKGHRDVIFGSKGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ08</accession>
    <name>TRUB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">MK0133</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ08"/>
    <dbReference type="SMR" id="Q8TZ08"/>
    <dbReference type="STRING" id="190192.MK0133"/>
    <dbReference type="EnsemblBacteria" id="AAM01350">
        <property type="protein sequence ID" value="AAM01350"/>
        <property type="gene ID" value="MK0133"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0133"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.132"/>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="OMA" id="GPFKEDE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="PTHR23127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000121960" description="Probable tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="247"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <sequence length="338" mass="37813" checksum="181744D10AF0AF33" modified="2002-06-01" version="1">MSGDKDRRLPFDRDREMITKAEVETDPRYGCPPEERPIEEYIMKGVINLDKPAGPTSHEVVAWVKEIFGLSKAGHGGTLDPKVTGVLPIALEKATKIIQTLLPAGKEYVTIMHLHGDVDEEELERVVKEFEGTILQRPPLRSAVKRRVRPKKIYYIDILEIDGRDVLMRVGCQAGTYIRKLCHDIGEALGVGAHMAELRRTRTGPFSEENAVTLHDVKDAYEFWKEEGWEEPLRHVVRPMEEGLEHLPRIEIRDTAVDAICHGANLAAPGIVRVEKGIQPGDLVAIFTLKGEAVALGVAKATWKEMLHADRGIMVDTKRVLMEPGTYPKAWGLKTPGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ11</accession>
    <name>OGT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="1">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ogt</name>
        <name type="ordered locus">MK0130</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ11"/>
    <dbReference type="SMR" id="Q8TZ11"/>
    <dbReference type="STRING" id="190192.MK0130"/>
    <dbReference type="EnsemblBacteria" id="AAM01347">
        <property type="protein sequence ID" value="AAM01347"/>
        <property type="gene ID" value="MK0130"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0130"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.129"/>
    <dbReference type="HOGENOM" id="CLU_000445_52_4_2"/>
    <dbReference type="OMA" id="QANRANE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000139378" description="Methylated-DNA--protein-cysteine methyltransferase">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O74023"/>
        </source>
    </evidence>
    <sequence length="131" mass="14714" checksum="54121FBA501B0646" modified="2002-06-01" version="1">MLEVTLPGEGTPPAEGFRTLHSLKRDLERYFRGYPVDFDDVPVRINVSGKPREVLELVREIPYGTVVTYGDIAQKANTHPRVVGVSLARNRVPIIVACHRVVAADGLGGFRWGLEWKRRLLELEGALPSRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ14</accession>
    <name>GLMS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber evidence="1">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmS</name>
        <name type="ordered locus">MK0127</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01344" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01344.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ14"/>
    <dbReference type="SMR" id="Q8TZ14"/>
    <dbReference type="STRING" id="190192.MK0127"/>
    <dbReference type="EnsemblBacteria" id="AAM01344">
        <property type="protein sequence ID" value="AAM01344"/>
        <property type="gene ID" value="MK0127"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0127"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.126"/>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_2"/>
    <dbReference type="OMA" id="ASEYRYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00164">
        <property type="entry name" value="GlmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000135425" description="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]">
        <location>
            <begin position="2"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 1" evidence="1">
        <location>
            <begin position="286"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 2" evidence="1">
        <location>
            <begin position="463"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="1">
        <location>
            <position position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="614" mass="68112" checksum="46E24A0CC30CE1FA" modified="2007-01-23" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ44</accession>
    <name>Q8TZ44_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0095"/>
    <dbReference type="EnsemblBacteria" id="AAM01312">
        <property type="protein sequence ID" value="AAM01312"/>
        <property type="gene ID" value="MK0095"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0095"/>
    <dbReference type="HOGENOM" id="CLU_080487_1_0_2"/>
    <dbReference type="OMA" id="WILLEYR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="RNA_MeTrfase_TK0422/Sfm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517">
        <property type="entry name" value="PTHR35517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="RNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="211" mass="24020" checksum="3C418D969E4F3661" modified="2002-06-01" version="1">MSVAIVEHLDRWSPWLDLEYMEAYRTARKHGWEFTVTNADPEVLAKAKYPALPYSASKLPLRGDRVIVLDPDAEDPLTPEDDPDVVIVGGILGDYPRRHRTREELTPRFPDAEVRHLGPYHFSIDGALRVALTVLECGVPLEEIQVVERPEIEVAPGHTVRLDCDYPSEGKPMLPEGLVEYLKEGIVDYEEAEFSARRRWRHRNDGVEGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ46</accession>
    <name>Q8TZ46_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA(His) guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">MK0093</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 5'-end ribonucleotide-tRNA(His) + ATP + GTP + H2O = a 5'-end phospho-guanosine-ribonucleotide-tRNA(His) + AMP + 2 diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54564"/>
            <dbReference type="Rhea" id="RHEA-COMP:14193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14917"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:141847"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.7.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA(His) guanylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.79" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0093"/>
    <dbReference type="EnsemblBacteria" id="AAM01310">
        <property type="protein sequence ID" value="AAM01310"/>
        <property type="gene ID" value="MK0093"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0093"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.93"/>
    <dbReference type="HOGENOM" id="CLU_044271_3_1_2"/>
    <dbReference type="OMA" id="WKQHTEI"/>
    <dbReference type="BRENDA" id="2.7.7.79">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008193">
        <property type="term" value="F:tRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025845">
        <property type="entry name" value="Thg1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024956">
        <property type="entry name" value="tRNAHis_GuaTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007537">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038469">
        <property type="entry name" value="tRNAHis_GuaTrfase_Thg1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12729">
        <property type="entry name" value="PTHR12729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04446">
        <property type="entry name" value="Thg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14413">
        <property type="entry name" value="Thg1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Thg1" evidence="12">
        <location>
            <begin position="13"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Thg1C" evidence="13">
        <location>
            <begin position="137"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM01310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="262" mass="30080" checksum="E2B91A564E7124B3" modified="2002-06-01" version="1">MKPVEPCDLEVYADLRVPPNTHLVLRIDGRAFTKLTRRLGLKKPYDRRFAEAMAETAVRMIRDAGLGITLVYTFSDELNALIPRGNVPFSGRVEKLTSVSASCASTYFFRALQRHGIDPTGETVSFDSRCVVLTDDDLVDYFKWRQDEAWRNHLNSYAYWALRERGLKPKEAAERLRGMKAHDVHELLYREFGINLGRTPAWQRRGILAYRVAVNEDGVQRRRVTRDWAPPFFDESEGERLIRACASQGYVSLDPAPDQVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ50</accession>
    <name>Q8TZ50_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0089"/>
    <dbReference type="EnsemblBacteria" id="AAM01306">
        <property type="protein sequence ID" value="AAM01306"/>
        <property type="gene ID" value="MK0089"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0089"/>
    <dbReference type="HOGENOM" id="CLU_656595_0_0_2"/>
    <dbReference type="OMA" id="ETWGAIQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036567">
        <property type="entry name" value="RHF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69754">
        <property type="entry name" value="SSF69754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="12"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="75"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="139"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="217"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="393" mass="44271" checksum="8AB4D50590DD79C0" modified="2002-06-01" version="1">MEIFERPVEEIMTPAEEVITAEPGEPLSKIFSKLERHGVKEIPIVDDGKVVGMISYYDVVDAHVADISNVRPETVMMKPETVTPDTLIVEAITEMIDSGLRALPVVEDGEFVGLVTEYDIIDVARESDELTKIDAREVMSTPVITIHENDTIAKARAIMRDHGISRLPVVNDANKLRGIVTTTDIIREVIKPITRLGKMDRKGEKVPAFGHPVKNIMSSPCVRAEPDETVVDLCEKIVEHGIRGMPIVNKLEEPIGVVTRRDILRKIPELMRKRGVFVSLKGVDDVDDFTLVILRKSIAAAVQKLASMRPSIEAVEVHIKRYHEEGNRHKYSVRIHVKDARNVISVKAHDWDLIIAFKNAIRHLVREVLGEEEKEETVRRKEAVKAKIKRRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ69</accession>
    <name>Q8TZ69_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Superfamily I DNA/RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0070"/>
    <dbReference type="EnsemblBacteria" id="AAM01287">
        <property type="protein sequence ID" value="AAM01287"/>
        <property type="gene ID" value="MK0070"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0070"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.71"/>
    <dbReference type="HOGENOM" id="CLU_001666_8_2_2"/>
    <dbReference type="OMA" id="YANDITF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00376">
        <property type="entry name" value="TIGR00376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_11" evidence="2">
        <location>
            <begin position="189"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_12" evidence="3">
        <location>
            <begin position="444"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="698" mass="79467" checksum="55250ECC50C93BEC" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ74</accession>
    <name>Q8TZ74_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0065</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01282">
        <property type="protein sequence ID" value="AAM01282"/>
        <property type="gene ID" value="MK0065"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0065"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.66"/>
    <dbReference type="HOGENOM" id="CLU_156335_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018676">
        <property type="entry name" value="DUF2149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09919">
        <property type="entry name" value="DUF2149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="125" mass="13826" checksum="1D23FD2F35D66BB4" modified="2002-06-01" version="1">MPRYLRRRRRRRFLDGGDNDDDPLTGVANLFDAAMVFALAFIITMSSSYGLTKILSPKTSEATIITKTTTGKVTVTKIYRTPSGLKVEQFKEVARAKRGGAQLGHMKKLGGAVYKAGKKYIWVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ90</accession>
    <name>Q8TZ90_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyruvate-formate lyase-activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0047</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0047"/>
    <dbReference type="EnsemblBacteria" id="AAM01264">
        <property type="protein sequence ID" value="AAM01264"/>
        <property type="gene ID" value="MK0047"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0047"/>
    <dbReference type="HOGENOM" id="CLU_835790_0_0_2"/>
    <dbReference type="OMA" id="CNAYRIS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="61"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="360" mass="40674" checksum="DF5E9B39BE88A202" modified="2002-06-01" version="1">MFGRLVIPGGCRKVLVRVWGGRVKDLRNCKLCAWECGVDRLEGERGVCRVTEPVIAAKQLHPAPPASYTVFMAGCNYRCLNCQNWDIAHYPDNPEGRALGYQDPKELAVEAVNMIETNQGRMIGADRIFFSGGEPTIHLPYIEQVVEHYRDTTDLWKVNFDTNGFATRKSMRRIVKLADSITFDFKAYSDPLHRAITGARVEPVLRNLEFLIPKYLDKIWEVRILLIPKAHDTEEIRAMCEFLADLDESVPVCFLAFRPNFVLERHPGAPKRLMERAVEIARECGLHATWSGMPGINGSVPPEVGECADKLLKHYDGRKGAALMGGYARVTGCRNHPRDCLACDDMARCPIKRYVAIRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ92</accession>
    <name>Q8TZ92_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted GTPase of the YlqF family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0045"/>
    <dbReference type="EnsemblBacteria" id="AAM01262">
        <property type="protein sequence ID" value="AAM01262"/>
        <property type="gene ID" value="MK0045"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0045"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.45"/>
    <dbReference type="HOGENOM" id="CLU_011106_1_2_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="107"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="367" mass="41278" checksum="83C8971A4743E698" modified="2002-06-01" version="1">MPPKWYRHVMRVLSESHVVLEVRDVRYPEETRWEKLPRLEDVFDFTRVVVLNKADLVPRAETERVKEEVELEEDVPAVYVSARERMGFRHLRRTIYEVAPEDVETVRVGVVGFQNVGKSTIINALTRRSAAETSRRAGYTRGKQWVRGGRKLLVIDSPGVIPTDEAAAEAVALDPDVLEDPVEPALGVIERVVREYPGALSDKFGIDESMDPERILRDISERLGKDLRTTAKLLLREWVDGSLVEIYRTTRADLAETSELEVGGTAQRLVEETLREIEEVVPEGIPPSAATVRGILTRLAHGENVDGVGFGTIRLGEYGVGVSVGDRYYDRMVRRLRRELGGEVISEERFRVGANGRKAVALVTKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ93</accession>
    <name>Q8TZ93_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0044</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01261">
        <property type="protein sequence ID" value="AAM01261"/>
        <property type="gene ID" value="MK0044"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0044"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.44"/>
    <dbReference type="HOGENOM" id="CLU_060920_0_0_2"/>
    <dbReference type="OMA" id="NAGFTCP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
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    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
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    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005911">
        <property type="entry name" value="YhcC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PTHR11135:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01091">
        <property type="entry name" value="uncharacterized_CHP01210-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01212">
        <property type="entry name" value="TIGR01212"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="16"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="38681" checksum="F1519AFCC34FABEC" modified="2002-06-01" version="1">MYEDGEKFAAFGAYYRREKGCKVMKAAVDAGFVCPNKDGRISREGCLFCPKMGRTIITPNVDPRKKLEEQARKQMEVFRERYGAEKFLVYFYPATNTYAPPDVLEELYNRALEMEDVVGLSIGTRPDCLPDEVLDILEGYVKEGYDVWLEIGVQSYHHRTLRRTRRGHGLAEVIDAITRAKERGIRIVNHIIFGLPGETRDEMLETVRVLSVLGVEAVKLYPLVVLERTDLERMYYDRRYKPLSYREYIRLLADALERMAPTVLIQRLSKDRAPDEERIEPEWDLYRMRVISDVRKELARRESRQGELYKVGLSAEELVPLVKGATGAERSGVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ99</accession>
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            <fullName evidence="5">5'-deoxynucleotidase</fullName>
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        </recommendedName>
    </protein>
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        <name evidence="7" type="ordered locus">MK0037</name>
    </gene>
    <organism evidence="7 8">
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        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
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    <dbReference type="EC" id="3.1.3.89" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0037"/>
    <dbReference type="EnsemblBacteria" id="AAM01254">
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    <dbReference type="OMA" id="YEKRDNI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HD_domain"/>
    </dbReference>
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        <property type="entry name" value="YfbR/HDDC2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="21367" checksum="3323EE9BDC737F49" modified="2002-06-01" version="1">MDVEAVYRLKRILRTGWLVRGIPRSSVESVAEHSFGAAMLAWEICHRLAERGIDVDPYKTVVMALIHDLPEALTLDLDVEASRVFGDAKREAEEKAAECVFDEELLDLWREFERRESPEAKAAKLADTLDMALQALEYSQVGFEAYREFLDSAEREARELGREYLLVFKEILRERGWDSNEGGGKGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA1</accession>
    <name>Q8TZA1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="gene ID" value="MK0035"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0035"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.35"/>
    <dbReference type="HOGENOM" id="CLU_762098_0_0_2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DUF1611-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DUF1611_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="UCP026760"/>
    </dbReference>
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        <property type="entry name" value="PTHR40690"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="50"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1611" evidence="1">
        <location>
            <begin position="152"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="363" mass="38919" checksum="326F8948C9D50E32" modified="2002-06-01" version="1">MTVLRSIDELAELNPFAVVGAGGGGEKFATLPTVEVVGIMDDDPSKVGMEIAGVKVTNDFDEAVEGASSVAIMLPKGAEHRALELAVESIRRGLNVVTSFRSLPLEDYPALVKLAESKGVKILELSPRLDVVREVAGDAPERCTEVVPKKDRPETEIPRVFVGGTSQECGKRTTTLKLAEGLEASGYTPATVATGEFAALEADVGFRAGSLSVMDVASAVAHAVDYVAEEKDADVIIVEGQSSLTERRNPHPRPLYLGILLGCAPDAVVVCHRPNHPFRYPRGVLAEVNALRVIVPDADLAAVCVNPRNLEEPFETYARRLRAKIWRATGEQVPVRNPVEESEKLARDVLRTIEGAGRGGARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA4</accession>
    <name>Q8TZA4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">50S ribosomal protein L19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rpl19A</name>
        <name evidence="5" type="synonym">rpl19e</name>
        <name evidence="9" type="ordered locus">MK0031</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0031"/>
    <dbReference type="EnsemblBacteria" id="AAM01248">
        <property type="protein sequence ID" value="AAM01248"/>
        <property type="gene ID" value="MK0031"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0031"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.31"/>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_L19/L19e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_L19/L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_L19/L19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_L19/L19e_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_L19e_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="RPL19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="PTHR10722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="SSF48140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Ribosomal_L19e" evidence="8">
        <location>
            <begin position="2"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="149" mass="17949" checksum="E74C84EB5CF819DE" modified="2002-06-01" version="1">MNLRPQRRMAAEILKCGVHRIWIDPERLEEVARAQTREDIRRLIKEGAIRKKPIKGQSRVRARKRHEQRKKGRQRGPGRRKGAKGARMPKKRAWIQRIRPIRRKLRELRDSGKIDRSTYRKLYMMAKGGYFRDTSHLLAYIEENDLWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA9</accession>
    <name>Q8TZA9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 15" type="primary">secY</name>
        <name evidence="15" type="ordered locus">MK0026</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0026"/>
    <dbReference type="EnsemblBacteria" id="AAM01243">
        <property type="protein sequence ID" value="AAM01243"/>
        <property type="gene ID" value="MK0026"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0026"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.26"/>
    <dbReference type="HOGENOM" id="CLU_031763_3_0_2"/>
    <dbReference type="OMA" id="KWGIGSG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="PTHR10906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="SSF103491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Plug_translocon" evidence="14">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAM01243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="458" mass="50198" checksum="7DA3DABF6853C543" modified="2002-06-01" version="1">MADDWLERLRPILERLPEVKVPDRHVPFNEKLFKYTGIPLILYFILCEIPLYGLSPQAVDYFANLRAVLAGNFGSILTLGIGPIVTASILLQLLVGGDLIKLDLTNPEDRRLFQGLQKLLAIVLCFFEGVMMVFSGAAPPAEPSILLEILLILQLALGGILVIFLDEVVSKWGIGSGVGLFIVAGVSSQIIIGAFNPLPSPQQPGRPAGAVWAFLYSAMQGTPDWTLLAPVIGAIITFLIVLYVEGMRVEIPIAFAGIRGARGRFPVRLLYTSNIPVILASALFMNVRLWALAFQRMGVPILGKLDPRGQPISGLVYYLSPPNSIVKTLSDPLQALGYMMAMVIASVFFAVLWVELTGMGPREIARHLHRAGLHIPGFRRDIRVLEKRLQKYIYPVTVMGGAFVGFLAAGADLMGALGGGTGVLLTVSILYNMYEEIKQERLMEAHPVVRKFLEKTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC2</accession>
    <name>Q8TZC2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-36 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0009</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01226">
        <property type="protein sequence ID" value="AAM01226"/>
        <property type="gene ID" value="MK0009"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0009"/>
    <dbReference type="HOGENOM" id="CLU_720825_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="383" mass="42877" checksum="31CF048C6353E533" modified="2002-06-01" version="1">MYVATWTGPGLDTDPNPETNCWTTSVWDVKWWNSHLDEVPVKEQIELIPVKDISRWRRILYTGVDPVTGHRIDVVYVPGGWRPEEHWGSDWDDLLRWAQLHLGIGYVGICAGATTFIRAFPCVWSVDGLKGHALPEVLLLPHPANPLVQEGESRVIRMYYANGPGMEWKRGWYTIKYYIDKKLHKIKAYCDVQGVMKYLEHVKGWAYVAGRWIVDGVEKGRFVVMSVHPEIMGRKDKDPDMVQTFIYAILWAAKRLPSGWKIHLTLDKTLVGVGEAVKVVLRLVNSVGAPIADFSPIWVKVVGPEGEEEHVLKTNEEGKATLTVVPESEGLYRIEASAVDVQATGSFSVGIPPVPFPVVTGVLIAAILGIVVTPRRVRQSSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVF3</accession>
    <name>F1SVF3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="7">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mcrB</name>
        <name evidence="10" type="ordered locus">MK0651</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="F1SVF3"/>
    <dbReference type="STRING" id="190192.MK0651"/>
    <dbReference type="EnsemblBacteria" id="AAM01866">
        <property type="protein sequence ID" value="AAM01866"/>
        <property type="gene ID" value="MK0651"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0651"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.690"/>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="OMA" id="TAMFEMG"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="F1SVF3"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MCR_beta_N" evidence="9">
        <location>
            <begin position="7"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="MCR_beta" evidence="8">
        <location>
            <begin position="190"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="444" mass="48241" checksum="A3DECC20A50E097C" modified="2011-05-03" version="1">MAREAKDTVDLYDDRGNCVAEEVPIEVLSPMRNEAIQSIVNDIKRTVAIDLEGIENALQNATVGGKGMKIPGREMDVDIVDNAEAIADEIEKMIRVYEDDDTNVEPMYDGKRLLVQLPSERVKVMADPYSGTLQAGMAVVHAIIDVCEVDMWDANMVKAAVFGRYPQTIDYFGGNVASMLDVPMKQEGVGYALRNIMVNHIVAATRKNTMQAVCLAATLEQTAMFEMGDALGPFERLHLLGYAYQGLNADNMVYDIVKKHGKEGTVGTVVREVVERALEDGVIEVKEELPSGFKVYKANDMDLWNAYAAAGLVAAVMVNQGAARAAQGVSATILYYNDLLEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFHGNHIVTRHSKGFAIPPVAAAMALDAGTQMFSPEVTSKLIGDVFGEIDEFREPMKYITEAAAEEAKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>O32865</accession>
    <name>MTRC_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit C</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrC</name>
        <name type="ordered locus">MK0658</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrC family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32865"/>
    <dbReference type="STRING" id="190192.MK0658"/>
    <dbReference type="EnsemblBacteria" id="AAM01873">
        <property type="protein sequence ID" value="AAM01873"/>
        <property type="gene ID" value="MK0658"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0658"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.697"/>
    <dbReference type="HOGENOM" id="CLU_092286_0_0_2"/>
    <dbReference type="OMA" id="HPFNACL"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01096">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005865">
        <property type="entry name" value="THM_MeTrfase_su_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04211">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006530">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01148">
        <property type="entry name" value="mtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147523" description="Tetrahydromethanopterin S-methyltransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="270" mass="27666" checksum="43BEFFCBEB6FABEB" modified="1998-01-01" version="1">MILRTLLISAVAPGGEEEEVEVAVAISPLKLMTAGLICGILGTAFAWVHPLIPALAVIPVVVWGADAVRRVAGYGLGTGVPSIGFMGLGGGSVAAILAAALSGNTVPAWAAAIIGTVIGAVVGALLGVLDRRVIKMKIPVMERCSTEIVASGTLALICLMAAVAGDFTWSAVYSKVIATGLIAVLWAICAISLLHPFNACLGPSETQERTLWLGAECGSLCTVVAGLATANPVVLLAGAAAWLITFWKFWELTKRDAADVVWTGIVPKGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>O32867</accession>
    <name>MTRA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="2">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mtrA</name>
        <name type="ordered locus">MK0660</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="2">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MtrA family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01875" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="2"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01875.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32867"/>
    <dbReference type="SMR" id="O32867"/>
    <dbReference type="STRING" id="190192.MK0660"/>
    <dbReference type="EnsemblBacteria" id="AAM01875">
        <property type="protein sequence ID" value="AAM01875"/>
        <property type="gene ID" value="MK0660"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0660"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.699"/>
    <dbReference type="HOGENOM" id="CLU_100863_0_0_2"/>
    <dbReference type="OMA" id="ARMKIVS"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000147504" description="Tetrahydromethanopterin S-methyltransferase subunit A">
        <location>
            <begin position="2"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="2"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="249" mass="26762" checksum="973CCF704F04ED9E" modified="2007-01-23" version="3">MPEKAEPAEGWPVVEGDYVVGDPEAPVHVVTLGSHIEEDILKAAGEDKVAIAGPCKTENIGIEKVIANVIANPNIRFGVLCGAEVTGHLTGQCFKAMYENGVDPDSGEIIGAEGAIPYLENIPEEAVERYRDQIVELVDLIDVEDVDEIVKAIEECVEKDPGAYEEGPMTISLEEEEEEELAEVAGMPVSAETVTVEYRINDVRVGVKSIGAMQRYMAGYLSGRTMGLLIGIISGMIFLFLPMVVLGGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>P50100</accession>
    <name>Y525_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MK0525</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>OrfX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0525</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1995" name="J. Bacteriol." volume="177" first="7238" last="7244">
            <title>Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri.</title>
            <authorList>
                <person name="Noelling J."/>
                <person name="Pihl T.D."/>
                <person name="Reeve J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8522533"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U31567">
        <property type="protein sequence ID" value="AAA92086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P50100"/>
    <dbReference type="SMR" id="P50100"/>
    <dbReference type="STRING" id="190192.MK0525"/>
    <dbReference type="EnsemblBacteria" id="AAM01740">
        <property type="protein sequence ID" value="AAM01740"/>
        <property type="gene ID" value="MK0525"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0525"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.559"/>
    <dbReference type="HOGENOM" id="CLU_040681_7_0_2"/>
    <dbReference type="OMA" id="YGICELC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106973" description="Uncharacterized protein MK0525">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="2">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAA92086." evidence="3" ref="2">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="3"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="196" mass="22197" checksum="9AC4960413652890" modified="2002-04-16" version="2">MRSVSVGEVARRDVITGSPTETAVEIAYKMREHGIGSVVIVNEKDEPIGIITERDLVIKVVSQGKNPDEVIARDIMSQPVITVEEDMEVNEAVKLMVDKGIRRLPIVDDNGKLIGIVTMQDILQVEPYLVATIEEEMKKFQEELEELEEVSEIIEGVCDLCETYSEELRFVDGVWVCPECYEDILGREIEDRELEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>P58832</accession>
    <name>Y400_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein MK0400</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0400</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58832"/>
    <dbReference type="SMR" id="P58832"/>
    <dbReference type="STRING" id="190192.MK0400"/>
    <dbReference type="EnsemblBacteria" id="AAM01615">
        <property type="protein sequence ID" value="AAM01615"/>
        <property type="gene ID" value="MK0400"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0400"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.427"/>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="OMA" id="DYMIVNE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094526" description="UPF0200 protein MK0400">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <sequence length="196" mass="22385" checksum="E7E227EF1200FAF8" modified="2002-05-27" version="1">MLICVVGMPGAGKGEFVKVAREEGIPVVVMGDAVRREAERRGMDVGEMAKRLREERGMDAVARLVEEDVERELRRAGVVVIDGIRNPEELEYFRDRFGERSVIVVAIHASPQTRFERLRIRGREDDPDTKREFEERDERELGFGIGDVISRADVMIVNERVSLPEFREKCRMVIRAILRGDPDDLPGGFDHLRVPD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>P58851</accession>
    <name>DTDA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-tyrosyl-tRNA(Tyr) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtdA</name>
        <name type="ordered locus">MK0939</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58851"/>
    <dbReference type="SMR" id="P58851"/>
    <dbReference type="STRING" id="190192.MK0939"/>
    <dbReference type="EnsemblBacteria" id="AAM02152">
        <property type="protein sequence ID" value="AAM02152"/>
        <property type="gene ID" value="MK0939"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0939"/>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="OMA" id="CYEATHH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="PTHR34667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000158967" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="279" mass="31379" checksum="49474EC38B012D85" modified="2002-06-06" version="1">MRRVVILWNPDDPASKNIAESLTEDAEKLDTEDLQHYTVETWERDGVRFHLTAALGDLIEEDEARELARKFDVIVFASRHESRTKKPSLTVHVPGNPTPEAKFGGKPLEVCTADPAGMKAALLELKRFRDKRGLDYDVCYEVTHHGPRDPGAPCFFIEIGSDEERWTDEEAGEACARAILAAVDPPDVKAVVGYGGGHYAPAHTDAALSNRKLAYGHIVPDYAVDHDYLRDQFREVVDKTPRAREIIVDDRNLDSGIVERLEDLVRDRGLRLRDVEEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q49606</accession>
    <name>MTRE_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit E</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrE</name>
        <name type="ordered locus">MK0656</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1996" name="Int. J. Syst. Bacteriol." volume="46" first="1170" last="1173">
            <title>Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons.</title>
            <authorList>
                <person name="Nolling J."/>
                <person name="Elfner A."/>
                <person name="Palmer J.R."/>
                <person name="Steigerwald V.J."/>
                <person name="Pihl T.D."/>
                <person name="Lake J.A."/>
                <person name="Reeve J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8863453"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-89</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="U57340">
        <property type="protein sequence ID" value="AAB02004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q49606"/>
    <dbReference type="STRING" id="190192.MK0656"/>
    <dbReference type="EnsemblBacteria" id="AAM01871">
        <property type="protein sequence ID" value="AAM01871"/>
        <property type="gene ID" value="MK0656"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0656"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.695"/>
    <dbReference type="HOGENOM" id="CLU_958513_0_0_2"/>
    <dbReference type="OMA" id="QMGNIHR"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01098">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005780">
        <property type="entry name" value="MeTrfase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04206">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016509">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01113">
        <property type="entry name" value="mtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147540" description="Tetrahydromethanopterin S-methyltransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="298" mass="31527" checksum="B540A568D1168C4C" modified="1999-07-15" version="2">MVGFTEIGLAAAMGALATIAGAFEDAESDVGSQSNPNSQVQLAPQMMNFHRYFNKAISGEPVSYMLYGAIAGTVTWVMMTKFGLPFLAAAAVGVGVNALIHMVFATTAHLGRMASAAEFGHPIYLDVVLSHLGPIAGFGGIATFAIVSLAYIQWALLKHPFPLPLLAALWGVTVGAIGSSTGDVHYGAERLYQHYPFGGGVPVAAHGNITRKAETGIRNSMDSVYFCAKFGNPLTGLCFGLVVFFSTWAGLFGQWGAVIAMGLVTLGCLIVSNRVEKKARESYGTYEDVEMDEICDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ4</accession>
    <name>Q8TGZ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0444</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0444"/>
    <dbReference type="EnsemblBacteria" id="AAM01659">
        <property type="protein sequence ID" value="AAM01659"/>
        <property type="gene ID" value="MK0444"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0444"/>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_2"/>
    <dbReference type="OMA" id="HAYVFEI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="173"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="264"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="452" mass="52465" checksum="DF9B28E430408627" modified="2002-06-01" version="1">MSSLSRQELTARVFVEWEETRRSLRECVKKVLLNEGVRDHRVWGTIHAYCFELMKRLRTVDAFLEATIRNAKLFDLDPWVRNALRVGTFEMKFNDVKPAIATNEAVKIVVECVGEGPARFVNAVLYDVERLELSDVLNRAKDTVERLAIEYSHPEWFVKHLMDLLGENELTKLMDANNREPERYLRVHAHMVDPDKAILALEDDGVAVEEDPDLPFMLRVVDHDVPPVRTEPYRRGWVAYQDKASAAAAYALRPEPGDRVLDACAAPGGKSAYLYALTEGEIELTCVDVNPRRLREMRRNFRRWGIEARLRRADSSRLYRETDETFDLALVDPPCSSSGAYVRAPEAKWTVKWRHVKRYARGQLSILRGVAPLVERTLVYSTCSVTVVEDESVVRRFLREFDEFEVEEPFGFGSPGFDSWMNERYPWADRVRRFWPHRHRTEGFFVARLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TH03</accession>
    <name>Q8TH03_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-Phosphoglycerate kinase fused to conserved uncharacterized domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0033</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0033"/>
    <dbReference type="EnsemblBacteria" id="AAM01250">
        <property type="protein sequence ID" value="AAM01250"/>
        <property type="gene ID" value="MK0033"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0033"/>
    <dbReference type="HOGENOM" id="CLU_884587_0_0_2"/>
    <dbReference type="OMA" id="IETDFIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02829">
        <property type="entry name" value="3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75500">
        <property type="entry name" value="SSF75500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="3H" evidence="1">
        <location>
            <begin position="210"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="37424" checksum="B8D55C6044DB2EDA" modified="2002-06-01" version="1">MARSTIVLIGGASGVGTSTIAREVARRLNITHLIETDHLREVLRGAIDKKYAPVLHMSSYNAYRALRIPDHMVPKRFRDRVIAGFVEHASMVKPAIDMVIKRAVEDASDLVIEGVHLVPGLVRPEDYQYADVHMVILYADEEEHRERFVKRAMEKGRGGRHLEYFRQIRIIHDFLLEAADEHGIPTIKNDDIDRTVSEVLSVVRSVSVVIKSVHSLEDSVREAEIIRENNCRLIDIGFPIPGKRDVIKVSFRRDPVDEWEEILNNEESRQYFKHLYSASNNVHYHKISAPDEESLHRAIEKLREEGFKVEELRPGDVCEVGKEDRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR4</accession>
    <name>Q8TUR4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1691"/>
    <dbReference type="EnsemblBacteria" id="AAM02903">
        <property type="protein sequence ID" value="AAM02903"/>
        <property type="gene ID" value="MK1691"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1691"/>
    <dbReference type="HOGENOM" id="CLU_1014190_0_0_2"/>
    <dbReference type="OMA" id="GYTTNCL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012121">
        <property type="entry name" value="ATPase_PP-loop_MJ1599"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036668">
        <property type="entry name" value="ATPase_UCP036668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="294" mass="33364" checksum="F05760A7D579AD77" modified="2002-06-01" version="1">MRQCRICGIPETVDGVELDESGVCRACRRQIRHIPSRKELREKVRKALENSEKVLVAVSGGLDSLAALGLALRICDEVVALTVDTGALHPEAWRRIILARRMSGIEWEIIGDQKPFLKLFEERLTRAESPCGPCSRMITRRYERRARELGVDAIVTGHELPHGTSPVVPKDPPVIRAMCGMTENERREIVEEEFGLTVDKISGYTSNCIVLPFALKLFYMKYGYSFEAPRLAAMVRYGYISREDMEQIMTPPTLSDLKELLRECEEWIPESLKQMLQKVINKISNSDLGRENGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT4</accession>
    <name>Q8TUT4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02882">
        <property type="protein sequence ID" value="AAM02882"/>
        <property type="gene ID" value="MK1669"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1669"/>
    <dbReference type="HOGENOM" id="CLU_2597748_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="9042" checksum="69F70C74D346E359" modified="2002-06-01" version="1">MRVSELLQRHVITNRGHDLGTVMEVELAWKDKCIKALLVQPSKEYAQQVKADVVEVPWSSVLAVGKYVLVDESKIRPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU9</accession>
    <name>Q8TUU9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase subunit of a ABC-type transport system involved in lipoprotein release</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1654</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02867">
        <property type="protein sequence ID" value="AAM02867"/>
        <property type="gene ID" value="MK1654"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1654"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1818"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="EVFCLIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="26184" checksum="C440821B52CAC4BC" modified="2002-06-01" version="1">MSNYVIRLENVRKVYNSGSVKVEALKGVSLGVKNGEFLMIVGPSGSGKSTLLHIMGALDTPTSGRVEIAGKDMTNLSDEELAEIRNKYVGFVFQQYNLIESLTVLENVMFPMTLAGEEDEERAKDLLRKVGLKEEHFDKFPSQLSGGEQQRVAIARALANDPEVILADEPTGQLDTKNSQRIMKLLEKLNDSGHTIVMVTHDLSLVRWADRVILLRDGRIEREMSPEELDAEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX0</accession>
    <name>Q8TUX0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02846">
        <property type="protein sequence ID" value="AAM02846"/>
        <property type="gene ID" value="MK1633"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1633"/>
    <dbReference type="HOGENOM" id="CLU_1324030_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="22450" checksum="0EFD0341EEEEC9D0" modified="2002-06-01" version="1">MVRPVSVGKHGGVSVEVLHPSDLPPQLTSLCKIKINGARVISRRSNIVAFRGRPSLEPLNDLDGFMSIAARESEVVMVGDPYLRVLAVEEGERIVVNTDRLLACLDFETIELKNDEITVVECVGPDIVVLACSSPVVASLKLEGDTAHVQAGSILCWWNIHTENVGDFLRLTGQGHVMLSITKKPDATEKGASTEKTTAPRKVSSWI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY2</accession>
    <name>Y1619_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-binding protein MK1619</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1619</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY2"/>
    <dbReference type="SMR" id="Q8TUY2"/>
    <dbReference type="STRING" id="190192.MK1619"/>
    <dbReference type="EnsemblBacteria" id="AAM02832">
        <property type="protein sequence ID" value="AAM02832"/>
        <property type="gene ID" value="MK1619"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1619"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1782"/>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="OMA" id="KPQFGEQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PTHR10840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="SSF46950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000121556" description="DNA-binding protein MK1619">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <sequence length="117" mass="14238" checksum="62CE31B2FCE4D9C5" modified="2002-06-01" version="1">MTDPELERIRRKKIMELQRKLEESQEKKVEEEREKKALEEAQRRAMLRRILTPEARERLARVRLARPQLAQAVENYLLQLAQTGQLKEKIDEDQLKRILKQVSDATRKEYRIRFKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV63</accession>
    <name>Q8TV63_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK1535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1535"/>
    <dbReference type="EnsemblBacteria" id="AAM02748">
        <property type="protein sequence ID" value="AAM02748"/>
        <property type="gene ID" value="MK1535"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1535"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1696"/>
    <dbReference type="HOGENOM" id="CLU_009116_6_0_2"/>
    <dbReference type="OMA" id="CAFERVE"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="303"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM02748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="45495" checksum="B2F671DA33094952" modified="2002-06-01" version="1">MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPILERHREFITEHLEQRHEEVESFLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASARIVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGTRSRLLRDLRSGRVPVVTGFIGRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEEAMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVALIRVSGAKMVDEPGVVGRVTSALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGLDYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGSAHGVVAVSVVVPESEVREVTRRLAERLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV78</accession>
    <name>Q8TV78_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator of the ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02728">
        <property type="protein sequence ID" value="AAM02728"/>
        <property type="gene ID" value="MK1515"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1515"/>
    <dbReference type="HOGENOM" id="CLU_097806_3_3_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="110" mass="12535" checksum="4ED54A330C3E5BE4" modified="2002-06-01" version="1">MLEPETFDVDPDIRPMSGHHDELVAVLKALGDERRIAALEILGHSEECLCKLAEILGENQPSTAYHLKKLLEVGLVTKRSEGGRTFYRLTERGERVLKVIRDLKDQLRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV96</accession>
    <name>Q8TV96_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02709">
        <property type="protein sequence ID" value="AAM02709"/>
        <property type="gene ID" value="MK1496"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1496"/>
    <dbReference type="HOGENOM" id="CLU_1084208_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="256" mass="28858" checksum="2B070F9B0F5C0B7B" modified="2002-06-01" version="1">MTVYVDATDILDPRWLFMGDPDRIYVFRTGHAPMHLHFELKRLVGFRVSVDGNAITGTVTPEGCLSSTCITPWVAQIVDARPGDDVLVVGGPEDGIDRVFELFPDVEEVEPEEPEPIREPHSNAVVMPNRKCKAARKYDTELKEILKKAGFEIVEPSVERYVCGFEAMQRTILRGDARIGIAASFDLVSGEYLGLIPVDVHGVVGTPRLNPELRVKTKDAPELLRNLLRMSPEHRREVYGQRLLEEPNWDRKFPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA1</accession>
    <name>Q8TVA1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted rRNA/ tRNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1491"/>
    <dbReference type="EnsemblBacteria" id="AAM02704">
        <property type="protein sequence ID" value="AAM02704"/>
        <property type="gene ID" value="MK1491"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1491"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1649"/>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="2">
        <location>
            <begin position="31"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="22003" checksum="CCC2F82E85A67551" modified="2002-06-01" version="1">MGVAVRDVVRYGRLKLLVFENVYPPAEDSFLLAEHQGVSGSERVLDVGTGCGIQGLSAAAKGCEVVATDVNPAAVHCARWNAHLNDLNIDVRVGDLFEPVRDERFDIVLFNPPYLPGRELPGSDPISRATEDPAVIRRFLKDLLREEIRWDEARIVVSSLTPKKYLEPLQRFEVEIVAEEPLFFEKIRVLALRPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA5</accession>
    <name>Q8TVA5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coenzyme-B sulfoethylthiotransferase</fullName>
            <ecNumber evidence="5">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mrtA</name>
        <name evidence="8" type="ordered locus">MK1487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1487"/>
    <dbReference type="EnsemblBacteria" id="AAM02700">
        <property type="protein sequence ID" value="AAM02700"/>
        <property type="gene ID" value="MK1487"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1487"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1645"/>
    <dbReference type="HOGENOM" id="CLU_2839385_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCR_alpha_N" evidence="7">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="65" mass="7650" checksum="B45631C7B8552927" modified="2002-06-01" version="1">MVERSKEEALFIKALKEKFEEDPEEKHTKFYVYGGYRQSPRKEFAKGALREFEPAGERDLIVPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA9</accession>
    <name>Q8TVA9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">folP</name>
        <name evidence="2" type="ordered locus">MK1483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1483"/>
    <dbReference type="EnsemblBacteria" id="AAM02696">
        <property type="protein sequence ID" value="AAM02696"/>
        <property type="gene ID" value="MK1483"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1483"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1641"/>
    <dbReference type="HOGENOM" id="CLU_041129_0_0_2"/>
    <dbReference type="OMA" id="DMIDLGM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045406">
        <property type="entry name" value="DUF6513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20123">
        <property type="entry name" value="DUF6513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00284">
        <property type="entry name" value="TIGR00284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="143"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="514" mass="57385" checksum="0D2C225F0802949F" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC7</accession>
    <name>Q8TVC7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">ubiX</name>
        <name evidence="7" type="ordered locus">MK1465</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1465"/>
    <dbReference type="EnsemblBacteria" id="AAM02678">
        <property type="protein sequence ID" value="AAM02678"/>
        <property type="gene ID" value="MK1465"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1465"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1621"/>
    <dbReference type="HOGENOM" id="CLU_074522_0_0_2"/>
    <dbReference type="OMA" id="GATHIQD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0288">FMN</keyword>
    <keyword evidence="1 5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="184" mass="20279" checksum="9CF0600FFCC2ACD4" modified="2002-06-01" version="1">MRVFIGVTGASGQIYARRLIEVLHEEGVDVEISVTRSAEYVMEQEGVDLPENVRRYDPKDLTAPPASGTYRIDAYIVCPCTLHTLSSVAVGVAGDLIKRAAVVALKEGRPLVFVVRETPWPRSALQAALKLREEGAVILPACPAFYHGPTTIEDLVDYVVQKVLDTIGVEVDLVRYQPRGMNFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD4</accession>
    <name>KAE1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kae1</name>
        <name type="ordered locus">MK1458</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVD4"/>
    <dbReference type="SMR" id="Q8TVD4"/>
    <dbReference type="STRING" id="190192.MK1458"/>
    <dbReference type="EnsemblBacteria" id="AAM02671">
        <property type="protein sequence ID" value="AAM02671"/>
        <property type="gene ID" value="MK1458"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1458"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1614"/>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="OMA" id="MRIMCEE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022449">
        <property type="entry name" value="Kae1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="PTHR11735:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303634" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01446"/>
        </source>
    </evidence>
    <sequence length="346" mass="37057" checksum="A5DF160ACAD49CEE" modified="2002-06-01" version="1">MICVGIESTAEKLGVGVVTDDGEILVNVKAQYIPPPGSGILPREAAEHHSRELPELLERALKNAGVEPEDIDLVAYSQGPGLGPCLRVGATAARTLALTLEVPLAPVNHCVAHVEIGKLAARQDGFDFDEPVTLYVSGGNTQVLALKAGRYRVFGETLDLPVGNMLDTFARKVGLPHPGGPEIERLAEEGEPVELPYTVRGTDVSFSGLLTAALRRYEQGDRLEDVCAGLQETAFAMLVEITERAAAQLGRDEILLTGGVAANRRLSEMMHEMAEDRGAEAYTVPPELAGDNGAMIAWTGILVHEHGLSIPPDEIPEKAIVKQRYRVDEAPVPWAARPSRSADSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI7</accession>
    <name>Q8TVI7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1402</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02615">
        <property type="protein sequence ID" value="AAM02615"/>
        <property type="gene ID" value="MK1402"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1402"/>
    <dbReference type="HOGENOM" id="CLU_1118218_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="248" mass="28183" checksum="181BBEC643BA9B5F" modified="2002-06-01" version="1">MRAVFYLLPLYIKSDDAWELGRLLRALDTRNRRVLVLDRARSFVHNLHQALRDAERIRSVEDLQQFAGQGGGIGVAEIIKGLVGADVHGLEAMVVCRTYLSNYAAQRSIIVPIMLHKIRLEPKDVFDDNPLDALRLEALESVLYDLAIVAGRAVRSEGDEYPFTVLLGAGTVVRVAQNYPDHAMEIYENFVETFKDELDPEVRVYDLGSKSEVPLEELEDVEVKKFEELSEDERKELVNWMNGQIAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ6</accession>
    <name>Q8TVQ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02545">
        <property type="protein sequence ID" value="AAM02545"/>
        <property type="gene ID" value="MK1332"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1332"/>
    <dbReference type="HOGENOM" id="CLU_1207627_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="229" mass="25132" checksum="D5E92EF955447F80" modified="2002-06-01" version="1">MGGRVEWWPEPPSGGRRARESGPAIGRSVLLLAIPLLLMVSPVSGVTVYVPDVVDGAFSLEKEGLEHATPVVTSGPVERFNTFENMVVDYEYVSVSDNHDLVVCSNPFDEIDVRITPEGIRVEYQTSRGTVRRSLSFNPEHTYLVLGLQGLRTIPVEPQQGGGELPVLIIHDHRVYEPTTYFPSPILGRYARDVTPLAQAVELMGGYDLLVRLEVDGSTVAFDLVKSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV1</accession>
    <name>Q8TVV1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02500">
        <property type="protein sequence ID" value="AAM02500"/>
        <property type="gene ID" value="MK1287"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1287"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="22508" checksum="BDFFA7E2C9A9A871" modified="2002-06-01" version="1">MERIRALRVLREVYRRGKKPGLEVTYRTVSGNTCGPYYVARWRRDSRFKHGRTLYLGKPENGSVRFVEWLVSLDREEVLELARHLMRNLRSVLKTLLTEVSGLPYKQARRVLARGLALAFDARPSESPRIRDVLEELPDRLESFTVRTLGGWPAHYSSHLKRVIRSRRRSLDGKHEVPDVGLELQRWKLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ2</accession>
    <name>Q8TVZ2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1246</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1246"/>
    <dbReference type="EnsemblBacteria" id="AAM02459">
        <property type="protein sequence ID" value="AAM02459"/>
        <property type="gene ID" value="MK1246"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1246"/>
    <dbReference type="HOGENOM" id="CLU_007415_2_3_2"/>
    <dbReference type="OMA" id="PIVECAG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="192"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="446" mass="46879" checksum="3E3199251F85ECEF" modified="2002-06-01" version="1">MKRLFYPEAVAVVGASRDPSKVGHGIFRNLLRDFDGPVYPVNPHADELLGRRCYPSLLELPERVDLAVIAVPARVVPEVVREAGEAGVPYCVVISAGFSEAGNEDLERELVRTARKVGVRLVGPNCLGIINARIGLNASFAAESPPPGNIAFVTQSGALATSVIDWFSSGDVGTVGVGFSKFVSLGNAADLDFPDFLEYLAEDDDTDVVVLYLEGVKDGPRFLDALSECAGRKPVIVLKGGRTEAGAEAVGTHTGSLAGSGEVYEGLVEGEGGIFVGGFEEAFDAAKLLAKAGPPTSDRVLIVTNAGGGGILATDALHELGFELPEPAGDPSVLPPEAATGNPVDVLGDADAERYRVALEEFADPDRYGAVMVILTPQSVTEPVKTARAVVEFVEEFPGPVIGVWMGRHSVALGIRVLEEGGVPTFPSPERAARALYHAARWAEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW40</accession>
    <name>ILVD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">MK1197</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW40"/>
    <dbReference type="SMR" id="Q8TW40"/>
    <dbReference type="STRING" id="190192.MK1197"/>
    <dbReference type="EnsemblBacteria" id="AAM02410">
        <property type="protein sequence ID" value="AAM02410"/>
        <property type="gene ID" value="MK1197"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1197"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1299"/>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000103542" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <sequence length="549" mass="59376" checksum="527E0229D33FA26D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW91</accession>
    <name>Q8TW91_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02358">
        <property type="protein sequence ID" value="AAM02358"/>
        <property type="gene ID" value="MK1145"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1145"/>
    <dbReference type="HOGENOM" id="CLU_1127095_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="26135" checksum="D9647DA5759412B3" modified="2002-06-01" version="1">MIEVLTVLLAHDPTLMDPCTHGLEVVVTGVRGAGRVGGGREETGGVGKGVGGGGRGGKVGGGGKTVHPSELLTKPKWRFLRETLGGGVKARRAHAFRWLTRAYHPLRSYYLSSLATAFTGLCVGLLAVPLATRARRWRPETVAFAAIALIVTVSTLCWEAFLGYLASCQFLARVTEGDAHLALSEFRTWGITGVGYLLAVYAVLASLLACAAWRKLGGRASLRLAPLLVLPFLTGLWFIRLGLAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW95</accession>
    <name>Q8TW95_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-17 family</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">MK1141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02354">
        <property type="protein sequence ID" value="AAM02354"/>
        <property type="gene ID" value="MK1141"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1141"/>
    <dbReference type="HOGENOM" id="CLU_1773197_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="16358" checksum="55950CE8A7AC0DB2" modified="2002-06-01" version="1">MVAILAPQLVVTGVTAYGLFILHIAWERRRKRAMDVLRGRGVEEKKPRSWEEPEEFKNSRCDPVTRLVFRPDLINCAPSGYLLVVSTMGWVALAFTYEMSVLLGGHSPPSVRFELVSTFAGLLVFSYRSDVATLLPGFRSPRSRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA6</accession>
    <name>Q8TWA6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1130</name>
    </gene>
    <organism evidence="1 2">
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        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02343">
        <property type="protein sequence ID" value="AAM02343"/>
        <property type="gene ID" value="MK1130"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1130"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1195"/>
    <dbReference type="HOGENOM" id="CLU_2366212_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="95" mass="10564" checksum="94C79CD806EDEC4E" modified="2002-06-01" version="1">MTARRFRPGTPVLLPVIRFFGRVIVTPFCFTVGREPVPCRVVVSYGRGAGPVDFRELKVESVNFPMCPVSLPLAVGFGVRVLGERSVYRRSGRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD1</accession>
    <name>Q8TWD1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02317">
        <property type="protein sequence ID" value="AAM02317"/>
        <property type="gene ID" value="MK1104"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1104"/>
    <dbReference type="HOGENOM" id="CLU_085612_0_0_2"/>
    <dbReference type="OMA" id="HRIVEAR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025328">
        <property type="entry name" value="DUF4234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14018">
        <property type="entry name" value="DUF4234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4234" evidence="2">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="28799" checksum="A2DBA9891CF794D6" modified="2002-06-01" version="1">MDPEIALQGLREHSTVYGEEDRIVPVWFAFFPQVLTFLGWLTIVMAKTFPGHATFLVLLGLLEALIGYPLAVYLMYLMIKRRNEHVTRSLGFLRYLVELLAGVGYDVDILRSELEEMRLHTERRNPVIYALLTLVPPIGWLVALYIYHFLNRDLHEHSVRERDFLESVARLLDMNPHEPPFELETFYVVPRRSTFLYFVLTLLTAGIFALYWWYTVVQDPNRHFRAHRRLERDLIESIEQAFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF6</accession>
    <name>Q8TWF6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase of the beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1079"/>
    <dbReference type="EnsemblBacteria" id="AAM02292">
        <property type="protein sequence ID" value="AAM02292"/>
        <property type="gene ID" value="MK1079"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1079"/>
    <dbReference type="HOGENOM" id="CLU_036012_1_0_2"/>
    <dbReference type="OMA" id="YYTCHCT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="2">
        <location>
            <begin position="50"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="26704" checksum="03E274B63CA03C4E" modified="2002-06-01" version="1">MYFSKERKSQFRSGVTASTGDSGSRGDVALKVTVVVSDEPPLDPELRSGPGFAALVEDGRHRVLFDTGPDPDLLLHNLKGLGYEPEDLTAVVISHNHWDHTGGLQAAESEVARIVTPERIGVKNELVREEWLGIGDMVLTDVLEGPPPERALYANGLLITGCAHPGIHEFVEWCVRRGLEVHTVLGGFHLMGATEIEVEKVADRLEELGVKIAGPCHCSGEVAKRMFRRRFEFLNVGPGLEVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI0</accession>
    <name>Q8TWI0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1054</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02267">
        <property type="protein sequence ID" value="AAM02267"/>
        <property type="gene ID" value="MK1054"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1054"/>
    <dbReference type="HOGENOM" id="CLU_896053_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="3">
        <location>
            <begin position="77"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="310" mass="35068" checksum="ACD1A4969516B90C" modified="2002-06-01" version="1">MSPRPYGGRFRSRRSCGSRGGSEAMEVTELEGLTISWDARPLAPDSVYGEDYMRIYRNVAGLLKIVQKAFDEPKGDLAIGFSGGKDSFTCALALEPFLREWDVNPKLVTVDVRVRGVEVWRPYRKELDRMAKALGYEFELVRAEEDVAEPSEELNKPPCKICSAIRRRILAERHDVVVYGHTAEDAIETLLMSVNRRRDAGTFPPADLLEEDDALLLRPLYVVSELRTARVYHEVTSRLGLPKVEPECPYAKRYRNSDSPRRTASELVERLREIARVRDELAVENLARGLARMALADVLCGRGRLEMPSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK3</accession>
    <name>PCNA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="1">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn</name>
        <name type="ordered locus">MK1030</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWK3"/>
    <dbReference type="SMR" id="Q8TWK3"/>
    <dbReference type="STRING" id="190192.MK1030"/>
    <dbReference type="EnsemblBacteria" id="AAM02243">
        <property type="protein sequence ID" value="AAM02243"/>
        <property type="gene ID" value="MK1030"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1030"/>
    <dbReference type="HOGENOM" id="CLU_043978_1_0_2"/>
    <dbReference type="OMA" id="EMKLINM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PTHR11352:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149197" description="DNA polymerase sliding clamp">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <sequence length="253" mass="28625" checksum="E96FE8AC9C7A3665" modified="2002-06-01" version="1">MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFDEYEPPSDELMYGLDMEEVTRIVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSIDIDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVGAKGNTFYFKAEGERGRVIPKVQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGEGKLTFYLAPRVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK7</accession>
    <name>Q8TWK7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1026"/>
    <dbReference type="EnsemblBacteria" id="AAM02239">
        <property type="protein sequence ID" value="AAM02239"/>
        <property type="gene ID" value="MK1026"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1026"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1077"/>
    <dbReference type="HOGENOM" id="CLU_843619_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="RNA_MeTrfase_TK0422/Sfm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="RNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="4">
        <location>
            <begin position="88"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="329" mass="37418" checksum="7E95D8F168D50355" modified="2002-06-01" version="1">MPLTPAEALLEILGTANVLVPRSYKPGKATGRAAVNRFALYAVRKGLGFEVEERPVRVYTSWRGLSLAVPGEETTSDYRATPRGRDVPPEELAEAVPDYPARPHFVVDYRFWGEHSDFGRTNLIRQTAVTASTLRLYLSDRHMSLVNVTPEAEERFLNAFPSFEGGLYEDWEDLIAELSVDRVILLDPNAEEELDENEIREDAVFVLGGIVDENMRGWTAKLAPGIPCDVERRRITLRGSIIGVPDRINALVEALSRVIVEGESLERAILRVQSPRFARRRLSRELRRLDRLTEEELRRLREVVNLPKREILLEARRRGIELKVDLQDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL9</accession>
    <name>VATE_METKA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">V-ATPase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpE</name>
        <name type="ordered locus">MK1014</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWL9"/>
    <dbReference type="SMR" id="Q8TWL9"/>
    <dbReference type="STRING" id="190192.MK1014"/>
    <dbReference type="EnsemblBacteria" id="AAM02227">
        <property type="protein sequence ID" value="AAM02227"/>
        <property type="gene ID" value="MK1014"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1014"/>
    <dbReference type="HOGENOM" id="CLU_105846_1_0_2"/>
    <dbReference type="OMA" id="YAGNIDC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="PTHR45715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000117317" description="V-type ATP synthase subunit E">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <sequence length="200" mass="23338" checksum="040ACBE7769ED1B0" modified="2002-06-01" version="1">MGVEELERKILEDAEKEAEEILEEAKRDAERIREKAEREAEEVRREILDRARREAETRRRREIAQAKLEIRQERLRVKEEYIEKAIERAEEKIRELAEEGRKEYLEFLKRSAIEAVNAISSDEVVLRANENDLMLLDEMLSEIRDETGKDVELGEPVEAVGGVIAESKDGSEAYDNTVDARLRRRRSEIVRRVSETLFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM3</accession>
    <name>Q8TWM3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane subunit of a Trk-type K+ transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trkG</name>
        <name evidence="8" type="ordered locus">MK1010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1010"/>
    <dbReference type="EnsemblBacteria" id="AAM02223">
        <property type="protein sequence ID" value="AAM02223"/>
        <property type="gene ID" value="MK1010"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1010"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1059"/>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="OMA" id="LQWMGGM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="490" mass="53631" checksum="D83833D86BB66749" modified="2002-06-01" version="1">MDVKLVSSILGTVVKVAAVLPLLPLPISLLYGELDCIAPLLLTSLIMLVCGVALERIARVERHVRPRHAIVAVPIIWLVIPLFTSLPYRFCEHMSWLDAYFESMSGWTTTGLTVLPDIDHAHRTILFWRSLEQWVGGLGIVVSIISILRFETPSLLYVAEAREERIRPNVVNTAKEMWKIYITITAVGFLALWAAGMNPFDALNHSMTAVATGGFSTRSESVTAWHSPTVELITVVLMVLGATSFVTHYRVAKTFRTSPAELPKRIIKALRQYLQDRQFLTMIGLTAAVTAYAGAVSGDRAWDVVRYYGYQAVSAITCCGFSNADVLSFPEYAKLAITILMIVGGSTASTAGAIKVLRFLIMAEAVKAAIARRTQPIRGVVVPRIGSRVLSEDEISDAFAIASAYMFFLLIGTLLLTAYGYPLVDALFEVASAQGGVGLSSGITAPNAPAFVKILLIFHMWIGRLEVLPCLAALYWLTLILKRLAVKGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN0</accession>
    <name>Q8TWN0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-keto-4-pentenoate hydratase hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mhpD</name>
        <name evidence="2" type="ordered locus">MK1002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1002"/>
    <dbReference type="EnsemblBacteria" id="AAM02215">
        <property type="protein sequence ID" value="AAM02215"/>
        <property type="gene ID" value="MK1002"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1002"/>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_2"/>
    <dbReference type="OMA" id="YALSIDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="65"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="264" mass="29725" checksum="938DB197D45F0C0E" modified="2002-06-01" version="1">MRVGTYLVDGTPRPAVFLDDEVHVYDLPVLEFIEVVHSDGLGDLDYSVYPLSELRIGPPVPRPPKIICFGLNYREHVEELREMGMDVPSEPVMTMKAPTAVIGHLDTVKLPREARRVDHELELAVVIGERCRKVSPEEARDAVLGFTIINDVTARDIEKREGQWVRAKSYDTFAPLGPWIETELEPDGLEMELRVNGEVRQRATTDDMVRDPYELVSFTSRVMTLEPGDIIATGTPPGVGPMEPDDKIELEIERIGRLVHYVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN9</accession>
    <name>Q8TWN9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mevalonate kinase</fullName>
            <shortName evidence="5">MK</shortName>
            <shortName evidence="5">MVK</shortName>
            <ecNumber evidence="5">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">erg12</name>
        <name evidence="5" type="synonym">mvk</name>
        <name evidence="8" type="ordered locus">MK0993</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0993"/>
    <dbReference type="EnsemblBacteria" id="AAM02206">
        <property type="protein sequence ID" value="AAM02206"/>
        <property type="gene ID" value="MK0993"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0993"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1040"/>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="OMA" id="NTVCTYG"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="PTHR43290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP_kinases_N" evidence="6">
        <location>
            <begin position="100"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP_kinases_C" evidence="7">
        <location>
            <begin position="229"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="328" mass="34969" checksum="5A7269C367A044B3" modified="2002-06-01" version="1">MRISVKAPLKAILAGEHAVVYGYPAVAVALDTYVRVTAEPGDDSFRVETELSCEGNVRAEITRDGNVKGFRSESLHEELTYVATAVRKASEEFDAPPSNLRILSEAPPASGLGTSAAVTAAVLLGLAEVSGVNVSREEIRRLVREVELEVQGKASWTDATVVTYGGFVRVSGREFELIEPERNPVLVVAHSQEPSRTGEMVRRVAELRERLDIVDGVMELIGELVDDLEAALRDGDLRTVGELMNANHGLLAALNVSTRALEEIVHVFRSAGALGAKVTGAGGGGCAVALFEREEDAKRAVETLSALGYEAFVTRPSPCGVKTEDSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR8</accession>
    <name>Q8TWR8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02177">
        <property type="protein sequence ID" value="AAM02177"/>
        <property type="gene ID" value="MK0964"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0964"/>
    <dbReference type="HOGENOM" id="CLU_1830605_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="140" mass="15088" checksum="6A52F6C693BAD150" modified="2002-06-01" version="1">MHPIESLDLALTALIAGLILTSEALRLFPFLSYLVRVPLRPITLVLALAALVLTFIAPIKGYVPTLLTLLCSVPKLSIGGYLLSRVWWVVSGLRDGLIGALRLSLEIRTLPFDLSVGCALTASGVLGVLRWMTSVPAVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT7</accession>
    <name>Q8TWT7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02158">
        <property type="protein sequence ID" value="AAM02158"/>
        <property type="gene ID" value="MK0945"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0945"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="24225" checksum="663CE1654436E48E" modified="2002-06-01" version="1">MITSHAHVPIPSDPVERIRALRVLREAYRRGKKPSLEVTYRTMGGSTCGPYYVARWRRDSRFKHGRTLYLGKPENESVRFVEWLVSLDRGEVLELARHLMRNLRSVLKTLLAEVSDLPYKRARRVLARGLSLAFNARPSESPRIRDLLEELPDRLESFLIRTLGGWPAHYSIHLRKVIRSRKRSLDGRYEIPDVGLELEHWRLRHGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU1</accession>
    <name>Y941_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized methyltransferase MK0941</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0941</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtxX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWU1"/>
    <dbReference type="SMR" id="Q8TWU1"/>
    <dbReference type="STRING" id="190192.MK0941"/>
    <dbReference type="EnsemblBacteria" id="AAM02154">
        <property type="protein sequence ID" value="AAM02154"/>
        <property type="gene ID" value="MK0941"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0941"/>
    <dbReference type="HOGENOM" id="CLU_086562_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016764">
        <property type="entry name" value="MeTrfase_MtxX_xsu"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019709">
        <property type="entry name" value="Methyltransf_MtxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03270">
        <property type="entry name" value="methan_mark_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000135934" description="Uncharacterized methyltransferase MK0941">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="254" mass="27505" checksum="E767DB7E04BF3815" modified="2002-06-01" version="1">MRVGIALSGDVPGAAERVLEAVERADVDVVVYHNVELDADVEEVRAKDPGRALVEDLVEGRLDAAVRGAVSGRCVRELVDALDLPFTGRSTVLEAEGRRVLLAPVGIDEGWEVESLVKLGELAARFHRRLTRREPSVAVVSSGRLEDFGRRSEIDRWLADGELVARLLKERGMEVEHVGILVEEALERDVVLFVNGVLGNLTFRCLSLVAGFRSHGAPVLAALERGVVFVDTSRAQRASGYARALRLAAELAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU8</accession>
    <name>Q8TWU8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted hydrolase of the metallo-beta-lactamase superfamily, contains a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0933"/>
    <dbReference type="EnsemblBacteria" id="AAM02146">
        <property type="protein sequence ID" value="AAM02146"/>
        <property type="gene ID" value="MK0933"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0933"/>
    <dbReference type="HOGENOM" id="CLU_798354_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="347" mass="38653" checksum="CA0676068A2290FC" modified="2002-06-01" version="1">MKLKIWSPVRLFGATPGAVVGISLTDIEVLFDPGGWRPGYNRRVPPWAELPEDLKSAPGLFSRFGEPYPDYTLVTHHHTDHAKHGGEYGEPFAHPGAAERIERRFDYRPEDPNRCEIVVETVETGHCPGAVAYLVELDGIRVLFTGDVSPETFLDARLPRADVLISECSGVPGHLDRVGLELLCRKAKPRIVVPVHLIAYDPWFVRELDVDAAVIEPHLGLSVDLEPALNAEVPCAREYHLCTRCERGRGCPVFRFVRHLRCPECGNPPTLDGEDPESVRLVCPRCGTRSNTVDYAEIIKAVAEYAMERSDVGDWSPTEARPGEYSSSERRLKEFSPRPGGGEDRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY5</accession>
    <name>Q8TWY5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S oxidoreductase fused to a metal-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02109">
        <property type="protein sequence ID" value="AAM02109"/>
        <property type="gene ID" value="MK0896"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0896"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.938"/>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_2"/>
    <dbReference type="OMA" id="IVWNFTN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034391">
        <property type="entry name" value="Cmo-like_SPASM_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01387">
        <property type="entry name" value="BtrN-like_SPASM_domain_contain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="110"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="468" mass="51734" checksum="B128EB3576105439" modified="2002-06-01" version="1">MTRVGEFIRVLNRVLDSELARRLMKLGAKRCESCGDVRVKVALDVAYGGREDACWKCKATARLVKKLVEKTARAAGVDEETVREALSLDSYRRGIAVTLLGIYRYGVRKPFVPAAPYLVVWDVTGLCNLRCEHCYSEAGKPAPGELDTERALEVIERFSEWNVPGLAFSGGEPLMRDDFFELAEASANEGMFTALATNGTLIDRDTAERLEAAGVEYVEISLDGARPETHDKFRGVKGAWERTVEGVRHCAETDMITVIAFTVHRNNVDELPQMLDLAEELGADGIAVFNFIPTGQGRFCPELDPPPEVREEVLKMLVREALDRDLMIYSTAPQMSRVSLQMTESGEGGVLYGTHFAYAGEGRWIEPLVEFIGGCGAGRCLLAIRPNGDVQPCVFLPVKIGNILEDDPEELWEHEVLWACRNRDELEKPCGECKYRYVCGGCRARAYAITGKVEGPDPGCVLVGDANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX20</accession>
    <name>Q8TX20_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein involved in rRNA processing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gar1</name>
        <name evidence="2" type="ordered locus">MK0859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0859"/>
    <dbReference type="EnsemblBacteria" id="AAM02072">
        <property type="protein sequence ID" value="AAM02072"/>
        <property type="gene ID" value="MK0859"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0859"/>
    <dbReference type="HOGENOM" id="CLU_165884_3_1_2"/>
    <dbReference type="OMA" id="HYAKQGF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="94" mass="11172" checksum="AF9409265FEECC80" modified="2002-06-01" version="1">MRRLGRALHVTPRGNLIARADHVPPLGAPVVTRRRERVGVIVDVFGPADKPYVAIRDKSRHHLERFVGKELFIPPRRRRRRPPRRRRGRPRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX37</accession>
    <name>Q8TX37_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted molecular chaperone distantly related to HSP70-fold metalloproteases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
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                <person name="Belova G.I."/>
                <person name="Aravind L."/>
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                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
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        <citation type="journal article" date="2013" name="Acta Crystallogr. D" volume="69" first="2440" last="2450">
            <title>Structural characterization of the ribonuclease H-like type ASKHA superfamily kinase MK0840 from Methanopyrus kandleri.</title>
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                <person name="Schacherl M."/>
                <person name="Waltersperger S."/>
                <person name="Baumann U."/>
            </authorList>
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            <dbReference type="DOI" id="10.1107/S0907444913022683"/>
        </citation>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Aravind L."/>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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        </source>
    </evidence>
    <sequence length="226" mass="25291" checksum="7FD243C29E15D407" modified="2002-06-01" version="1">MTDSRSTEVSKLVELLPGFHCGACGYDRCDDFAEALVRGEAKLDDCPYLQTERFAEERRRLEKLLEEIGEAEGARPSITGVLDGYEADLVLSPLPDEPACREILHPFWSEADIEEGDVIRYRPWGCPTTHFARVLDAEKGLLTVHIVGPRHRLGETDFEYKDVGLCLVVGFEGIVSEGRVPNVGETVRFVPEHCMMQKVHSGVVIEAVGDRLRIECIDLKVWAPPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX79</accession>
    <name>Y796_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0288 protein MK0796</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0796</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0288 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX79"/>
    <dbReference type="STRING" id="190192.MK0796"/>
    <dbReference type="EnsemblBacteria" id="AAM02010">
        <property type="protein sequence ID" value="AAM02010"/>
        <property type="gene ID" value="MK0796"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0796"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.837"/>
    <dbReference type="HOGENOM" id="CLU_533841_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01089">
        <property type="entry name" value="UPF0288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005543">
        <property type="entry name" value="PASTA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016466">
        <property type="entry name" value="UPF0288_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005852">
        <property type="entry name" value="UCP005852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03268">
        <property type="entry name" value="methan_mark_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51178">
        <property type="entry name" value="PASTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156052" description="UPF0288 protein MK0796">
        <location>
            <begin position="1"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01089"/>
        </source>
    </evidence>
    <sequence length="511" mass="57627" checksum="919CD3F60C8EA9A5" modified="2002-06-01" version="1">MRVYVDGEPVDVPEEATVRDALEAAGVSVPEDVTIAVFKGEQKVERETDRLRIMLETGDEELSLTVAVEDERMSEVCEELPGASVSWTTRDEVGLGPVDVSDLEFHTRRGVEVPPYTAILILPTNDPSEAYFLITKRRMAVEYICTDIHGRVTAGRELVDELRGGERVTHVEPVVERATERVVSRVTLDDGLEAGDRIITRVEIELEKNAPVSAEHLLNTLEMEEGRLRIKFRTDTFTSIEPRPFYDLPEENVDMRERGVVTVRNRGVDEGVVYVYRRDRTPVESHNVVGRVRRGMELLDVVAEGDRVLVETDPPRVNFVGLTVDEARELAEEFDVELEVNGDGDVVVDQEPRETLNVLKERKVRVEVVPEDEVIEIELYEDDAPRSVEYFRRVTKMLDRPVGRLKVHFAYADLGMIVFEGNEKLGKKLPPENNPKDRVEAGVLGVTNQAKPHAGLIGVRLEDSEEYGPTGETFEGTNVIGRVVEGLGRLREMDQSDMGRTVYVREVRGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX97</accession>
    <name>Q8TX97_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiamine biosynthesis ATP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thiI_1</name>
        <name evidence="4" type="ordered locus">MK0778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0778"/>
    <dbReference type="EnsemblBacteria" id="AAM01992">
        <property type="protein sequence ID" value="AAM01992"/>
        <property type="gene ID" value="MK0778"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0778"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.819"/>
    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiI" evidence="3">
        <location>
            <begin position="156"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="348" mass="38940" checksum="C46581ABA51EA8CB" modified="2002-06-01" version="1">MKPVTLIRPSGEVTAKSAPVRKTLLGELARRLERRLGEVVRVRGPRLIVPGHHPNAASEFGVEGALPGYKVRPKPATILKATRRALRECPEEIRVDVRSHHDGVSGHALFESVREIVESTGRKTSKRGSRLLVEVYPDVAYVCGPEKAGPGGLPVGTQGRALCLLSGGFDSPVATWMVMRRGLECKGLHFLVTESELEAARENERVLRRWCPDFDLLVDESHREFLETAEERLTGRLKRYLCVVCKMRMLERASQWADRLGCDCIVTGDSLGQVASQTVWNLKLEESQVNGIVLRPLVGLNKPEIVRYGRRIGVPERDPGRCPFVPERLIVKPRKREALRAYREVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA1</accession>
    <name>Q8TXA1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Geranylgeranyl pyrophosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ispA</name>
        <name evidence="3" type="ordered locus">MK0774</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0774"/>
    <dbReference type="EnsemblBacteria" id="AAM01988">
        <property type="protein sequence ID" value="AAM01988"/>
        <property type="gene ID" value="MK0774"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0774"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.815"/>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="36063" checksum="770871114AB1DF04" modified="2002-06-01" version="1">MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLGGSEEDVLPEAVAVELIHTFTLIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAFEVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTEDEFLEMIRKKTAALIRTSCVVGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIVIKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAALRELPENEHREFLEKLADFVVEREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB1</accession>
    <name>Q8TXB1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0763</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0763"/>
    <dbReference type="EnsemblBacteria" id="AAM01977">
        <property type="protein sequence ID" value="AAM01977"/>
        <property type="gene ID" value="MK0763"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0763"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.803"/>
    <dbReference type="HOGENOM" id="CLU_100862_1_1_2"/>
    <dbReference type="OMA" id="TIAYVTR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00288">
        <property type="entry name" value="TIGR00288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="2">
        <location>
            <begin position="24"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="179" mass="19858" checksum="BA0007C70151DCA3" modified="2002-06-01" version="1">MLDTVKDYMSGVIKRKAKRGNLDIAMLIDGPNMLRKEFDVSLKEVRELVEELGNIRVGLAFLNQYASDKLIEAVANQGFVPRVIPGDVDVYLAVEAMELIYSDNVDAIALMTRDTDFLPIIAKAKEQGKVTIVIGADPGFSTALQNAADYVIKLKPRKERESEGKEEKPEEAKDVVSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB9</accession>
    <name>Q8TXB9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylmuramate-alanine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">murC</name>
        <name evidence="3" type="ordered locus">MK0755</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0755"/>
    <dbReference type="EnsemblBacteria" id="AAM01969">
        <property type="protein sequence ID" value="AAM01969"/>
        <property type="gene ID" value="MK0755"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0755"/>
    <dbReference type="HOGENOM" id="CLU_012007_0_0_2"/>
    <dbReference type="OMA" id="EAAFKYK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="114"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="1">
        <location>
            <begin position="358"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="512" mass="55771" checksum="8180835918728179" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD4</accession>
    <name>PDAD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="1">PvlArgDC</shortName>
            <ecNumber evidence="1">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdaD</name>
        <name type="ordered locus">MK0740</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + L-arginine = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXD4"/>
    <dbReference type="SMR" id="Q8TXD4"/>
    <dbReference type="STRING" id="190192.MK0740"/>
    <dbReference type="EnsemblBacteria" id="AAM01954">
        <property type="protein sequence ID" value="AAM01954"/>
        <property type="gene ID" value="MK0740"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0740"/>
    <dbReference type="HOGENOM" id="CLU_114389_2_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PTHR40438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="SSF56271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000023316" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000023317" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="1">
        <location>
            <begin position="42"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="1">
        <location>
            <begin position="41"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <sequence length="150" mass="15596" checksum="8FBA7ED4CCB07181" modified="2002-06-01" version="1">MGVPKKYALVSGTGEADTSLAAFDAALIDAGIGDCNLVELSSILPPNAEEDDLPEFPPGSIVPAVVAKAVGRGLVSSCICVGRLESGLGIVSERAATDSVETVRRLAKRDVEEMARLRGEKLVEVRTVTASTEPEDAEWAAAVAAVVFWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG7</accession>
    <name>Q8TXG7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATPase involved in pili and flagella biosynthesis, VirB11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0707</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0707"/>
    <dbReference type="EnsemblBacteria" id="AAM01921">
        <property type="protein sequence ID" value="AAM01921"/>
        <property type="gene ID" value="MK0707"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0707"/>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="OMA" id="TGHDGCS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="259"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="602" mass="68898" checksum="BDA19E3A15F83C49" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM0</accession>
    <name>GGGPS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">GGGP synthase</shortName>
            <shortName evidence="1">GGGPS</shortName>
            <ecNumber evidence="1">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0642</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXM0"/>
    <dbReference type="SMR" id="Q8TXM0"/>
    <dbReference type="STRING" id="190192.MK0642"/>
    <dbReference type="EnsemblBacteria" id="AAM01857">
        <property type="protein sequence ID" value="AAM01857"/>
        <property type="gene ID" value="MK0642"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0642"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.681"/>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="OMA" id="ADAIFFM"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138733" description="Geranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="167"/>
            <end position="173"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <sequence length="239" mass="25052" checksum="179093DDD7872137" modified="2002-06-01" version="1">MGVFGYLRERTPCHLTLLDPVDVGPEEVPEVLESLVKAGTDAVMVGGSTAHASQVEAVVEAIREVADVPVILFPNGPEGLAPNADAVLFMSLLNSRNTYYLIEAQVKGAPLVERFGLESIPTGYLIVGEWGTTVSVVGDARVIPFDRVEVIVAYALAAKHLGMKAVYLEAGSGAPEPVPPEVVRRVSGIGVFTIVGGGIRSPETARELAEAGADAIVTGTAVERDPDLAAEIVEAIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS7</accession>
    <name>Q8TXS7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea, related to methyl coenzyme M reductase II, operon protein C (MtrC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0583"/>
    <dbReference type="EnsemblBacteria" id="AAM01798">
        <property type="protein sequence ID" value="AAM01798"/>
        <property type="gene ID" value="MK0583"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0583"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.618"/>
    <dbReference type="HOGENOM" id="CLU_890295_0_0_2"/>
    <dbReference type="OMA" id="AKPNMIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011312">
        <property type="entry name" value="UCP019164_menthan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019164">
        <property type="entry name" value="UCP019164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03274">
        <property type="entry name" value="methan_mark_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="308" mass="35114" checksum="3B530C8C34B34B3E" modified="2002-06-01" version="1">MGVASVFETVIYEGGVYKADEMRDLVEDLGGFVLSEHQMQLEVHMVLAVPVDDLDVIERKAEELLGEIRRMPLAGTEIAVVGMSLARHHMPHPVCDIAEYLRRHGAKSNVIGLARGYGRELAQLRTREKRLLEEHDLVIFVVGNFKDCIEKYKLPLVRRIERVPVVVVGGPEEIDADDVVYVGGIGRKPYRFRKGREITKLDEIVDVVSKIIEERRQELAEDPPVVPPMVVKDRINRELKIEERFTSPMPITLQLDGLRVKAPYDEWADRVAEVEVFGWKLREIADIRRSEMDDHILVKIRPKSAVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU3</accession>
    <name>Q8TXU3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01782">
        <property type="protein sequence ID" value="AAM01782"/>
        <property type="gene ID" value="MK0567"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0567"/>
    <dbReference type="HOGENOM" id="CLU_1017846_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="273" mass="28016" checksum="20A2808B50C72A4E" modified="2002-06-01" version="1">MIAEVAEFVLEALLVIGVSVFGLHLLGALASEKIRCRAVPLVVVGLIHPHAALTTAREMGLKGRELLIAKVSFNSATYTATACVNALTLLGSTVGGPYALTLALVDATETIVGGLLLRRVRAQLPTHGSLRDALNEATRTVGRIAAYLVPGALLGIILSSYLRGLGGYVVPMTFLANPVAGCAAVGTMLRSGVLDYAHAYSLAVTGAALAYVGRLLRSCGPVTVAITGLRSGSLLSTVNLLADIGLLLVYGMVIGYLTFGPPGVPPLGVLGGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW5</accession>
    <name>SYT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrS</name>
        <name type="ordered locus">MK0544</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged L-seryl-tRNA(Thr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain is an archaea-specific tRNA-editing domain that hydrolyzes incorrectly charged L-seryl-tRNA(Thr). Catalysis of tRNA editing is performed by the charged tRNA itself.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXW5"/>
    <dbReference type="SMR" id="Q8TXW5"/>
    <dbReference type="STRING" id="190192.MK0544"/>
    <dbReference type="EnsemblBacteria" id="AAM01759">
        <property type="protein sequence ID" value="AAM01759"/>
        <property type="gene ID" value="MK0544"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0544"/>
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    </dbReference>
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        <ligand>
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        </ligand>
    </feature>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="624" mass="71462" checksum="D0474BFB4A81187D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX0</accession>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
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                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="275" mass="30220" checksum="9D38084273E8F1DE" modified="2002-06-01" version="1">MGEYREVYLTRATDERIREHGQDGGTTTALLAHALEEDTVEAVIASSTVETWKPEPVIVTDPDELIETAGSKYAISPNVSALNEAIASYDSVALVGTPCQITAVKKSKMYPYGLANVTERVKLTVGIFCTENFQYESLLKLLEDMGVDVENVERMDISHGEFIVRTKNGDVHSVSVSKLGDYANEACNYCTDFTAEDADISVGSVGAPDGWNVVLVRTKEGEKVFRSAVDADVLEVKDIGEGDPNLLERLARDKKERIHTSMCATWRPYHPTVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY3</accession>
    <name>Q8TXY3_METKA</name>
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        <submittedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">MK0526</name>
    </gene>
    <organism evidence="3 4">
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
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            </authorList>
            <dbReference type="PubMed" id="11930014"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="CBS_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="68"/>
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    </feature>
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        </location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="278" mass="30993" checksum="C550F91E2BD229C4" modified="2002-06-01" version="1">MPDTSVMRLATTNVVSMPPTATVKSAVDTMIRYGFRRIPVTEPGELELVGIMTGKDVLDYLVGERRKIIERRYGSTFLPALHEPVRSLMRTEVYVITPYDTVRKAVRTMFEFEVGALPIVKDKKLVGIITERDIMADLYDVLEDTRVEEIMTEDPETVPSDITVLEAAEIMVDREFRRLPVVENGRLCGLVTATDVLHHVSSMATETSPDASVEEVMDVPVEEIMTEDVITIEPDVNIEEAALTMKGANVGSLVVTEGNDVIGIITERDIMYAIAERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY08</accession>
    <name>Q8TY08_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanopyri</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01713">
        <property type="protein sequence ID" value="AAM01713"/>
        <property type="gene ID" value="MK0498"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0498"/>
    <dbReference type="HOGENOM" id="CLU_023081_3_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="308" mass="34741" checksum="F64ECB0A3DE3F95E" modified="2002-06-01" version="1">MERIRARLNLGETLLEWERVLARCDGCGACESRCPNDVPRRLFNVLAASGRFEEALSVASDCTVCGLCERLCPHNIPYTEVITDLRREEPTPEAVRRVRRTGTPYGRKLADESEPLQGDGVAILGCTIRSRPEWLRRVIEIVRRAGLATLGEDEPCCMNFARKRGEEVPEETVQRWCSLFDDFDTVVVFCPGCYDFCVEVLDERPLYYAEIAEFDGIPEGAAYKSPCHLVRHGVDDHVLERLPDVELPPRRHRCCGGGALVRKPSESTVRAYERLGKPVLTPCPMCVTTLEGVIDVRPLWDHVVDLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY64</accession>
    <name>Q8TY64_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01656">
        <property type="protein sequence ID" value="AAM01656"/>
        <property type="gene ID" value="MK0441"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0441"/>
    <dbReference type="HOGENOM" id="CLU_2730399_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="71" mass="8207" checksum="B798F69BF39F6EC1" modified="2002-06-01" version="1">MHWIAEGWARIRVKVAASNSGKVLRVLDISWHYDKEERREERQERGVPVGAVASLTGVVLAWMLRSSRSRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY98</accession>
    <name>Q8TY98_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0407"/>
    <dbReference type="EnsemblBacteria" id="AAM01622">
        <property type="protein sequence ID" value="AAM01622"/>
        <property type="gene ID" value="MK0407"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0407"/>
    <dbReference type="HOGENOM" id="CLU_027052_1_0_2"/>
    <dbReference type="OMA" id="IDPGTTT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007408">
        <property type="entry name" value="DUF460"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40707">
        <property type="entry name" value="PTHR40707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04312">
        <property type="entry name" value="DUF460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="405"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="665" mass="77210" checksum="46E128F0A802E4E0" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA4</accession>
    <name>Q8TYA4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01616">
        <property type="protein sequence ID" value="AAM01616"/>
        <property type="gene ID" value="MK0401"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0401"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.428"/>
    <dbReference type="HOGENOM" id="CLU_061722_0_0_2"/>
    <dbReference type="OMA" id="VIQQGMN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008482">
        <property type="entry name" value="DUF763"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38597">
        <property type="entry name" value="PTHR38597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05559">
        <property type="entry name" value="DUF763"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="357" mass="40121" checksum="EFA764F8E6F2B117" modified="2002-06-01" version="1">MGEPAAVPRAAELRLVKCPPPRHFPEMVKLSRALFRLLVEEHGTDGALEKLADPRWFQSLACLLGYERNTSGSTTVVTAALREALDPEEHGIAVAGGKGRLALETPKRVRELADRMNLDPRQLVTASRLTARSDSVCLQDGHDLYHHVIVFDENGRWVVIQQGMDVDRKTARRYHWLDSEVSEFVEGHPVVADETRKVLSLQGDRADKCREAVLDLVGDGPDRVLREWRAVRNSISGPLDEYLGREGGVEVPDGWVPRRLDRDALRRLYEVNPTDFKEFLTVRGVGPSLVRALALIAEVVYGEGPDRRDPAEYTAAFGCKSGDPYPVHRELMRLAAELLERIRSPRLRRFLGRVTES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB0</accession>
    <name>Q8TYB0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">L-sulfolactate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">comC</name>
        <name evidence="3" type="ordered locus">MK0392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0392"/>
    <dbReference type="EnsemblBacteria" id="AAM01607">
        <property type="protein sequence ID" value="AAM01607"/>
        <property type="gene ID" value="MK0392"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0392"/>
    <dbReference type="HOGENOM" id="CLU_040452_3_1_2"/>
    <dbReference type="OMA" id="TNTEPAM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="PTHR11091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="SSF89733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="341" mass="36769" checksum="4FD6CB8FBCD832C8" modified="2002-06-01" version="1">MKIRPEDLTDFVSEALHAVGVPRKDARTAAEVIVEGDLRGFHSHGVLRLPGYIEGIKRGAIRPEMRIEEISRKSSVVLYDADHSLGHVVGYRATLEAVELARKHGLGMVAVRNASHYGIAGYYTTLVAERGFIGFTTCGTEPAVAPYGGSQPVLGTNPVSIAFPRRDGPPIVVDMATSVVARGKILQALRENREIPQDWAVGPDGEPTTDPEEALEGALLPFGGHKGYALCLALEVLAGPVVGAAAGKDVQGTTDPTVPCNKGDVFVALDLSTLVDEHEYYERLERLISQVKSAGDDVLLPGEPEFRRRERALREGIELPEGSVRAVREVAEELGLEDPTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC0</accession>
    <name>RRP4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp4</name>
        <name type="ordered locus">MK0382</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Confers strong poly(A) specificity to the exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC0"/>
    <dbReference type="SMR" id="Q8TYC0"/>
    <dbReference type="STRING" id="190192.MK0382"/>
    <dbReference type="EnsemblBacteria" id="AAM01597">
        <property type="protein sequence ID" value="AAM01597"/>
        <property type="gene ID" value="MK0382"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0382"/>
    <dbReference type="HOGENOM" id="CLU_071769_0_0_2"/>
    <dbReference type="OMA" id="GPYIPEV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00623">
        <property type="entry name" value="Exosome_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023474">
        <property type="entry name" value="Rrp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PTHR21321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000050147" description="Exosome complex component Rrp4">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="67"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="151"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00623"/>
        </source>
    </evidence>
    <sequence length="239" mass="26914" checksum="032E1655DC3E36B8" modified="2002-06-01" version="1">MPEFELYVEDRQVVTPGELLARGQVIASEGTYTSGDEVYSKVTGLVDIDGRRIRVIPLAGPYRPSPGDFVVGIVEEVKFSSWLIDVRAPLPAILHVSNALEEEVDLIETDLSRYYRPGDVITAVVREVDPVQRVELSLLEDDAPTRLGRLQGGQVVEIDPVKVPRVIGRKGSMIKMLKRVLGCDIVVGANGRIYVRAREEPKKERELLAVRAIREIERRSHLRGLTDWLKANLKRLSRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD3</accession>
    <name>Q8TYD3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01582">
        <property type="protein sequence ID" value="AAM01582"/>
        <property type="gene ID" value="MK0367"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0367"/>
    <dbReference type="HOGENOM" id="CLU_627921_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="436" mass="49207" checksum="9DABCB43777E672C" modified="2002-06-01" version="1">MTFTALTGVYSMQYGELVETYRVTLGDKTYEVPVEYYVWKNGNGHFVWVSLGVHPLEYQAHKCMFEAVKWFVYNSGEFEGTLVVSWIKIPAEYYQSGSEEEKYRVSRDLGQLTFYNHVLPYVLHPKELGSKVGVPITRKPELFLDVHAHRPSWGVSEFILIPAGPIHGNDNPESFQRALQICEVLAGVIGARVDTQQTGTSPPWVTAPVAKHGIPAATFENGYYYIGQELPPNYDNYRTELDKAFLEALYLYALEGGRVPPTPEGYRKLARWYAMAAETWGRTVEYLSKAAETHPEYRSELQRLAEIAAKIRDLLVEMSEECKRVAELLERGQRDEAARLVDSELIPEYEEVTRLYDEYISGTKRIPVRISDVFASEEHESGDYIEVFGVKLPVSLAATIAGAGILAAVITFKVSVNPLVGLIACGALLWLSRRWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD9</accession>
    <name>Q8TYD9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK0361</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0361"/>
    <dbReference type="EnsemblBacteria" id="AAM01576">
        <property type="protein sequence ID" value="AAM01576"/>
        <property type="gene ID" value="MK0361"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0361"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.383"/>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="OMA" id="QLMTNTH"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="17370" checksum="6D29F0190071C471" modified="2002-06-01" version="1">MTRYKVGLADTTFARVDMASVAEKVLKEHLPGVETPRYTVPGIKDLPVACKRLIEEEGCDLVMAFGMPGPTEIDKQCAMVASMGLILAQLLTNTHIIEVFVHEDEAEDERELHELAVKRAEEHALNVVKMLEKPEALTREAGMGRREGHPDVGPARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF6</accession>
    <name>Q8TYF6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted 23S rRNA methylase containing THUMP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0344"/>
    <dbReference type="EnsemblBacteria" id="AAM01559">
        <property type="protein sequence ID" value="AAM01559"/>
        <property type="gene ID" value="MK0344"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0344"/>
    <dbReference type="HOGENOM" id="CLU_775255_0_0_2"/>
    <dbReference type="OMA" id="NCVVDIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="54"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="357" mass="40218" checksum="FD4134ED23AA4A1B" modified="2002-06-01" version="1">MIPPFQALVTCPAGFEFRCVEELEDILKEQDPYAEAEPTYFKGVVVVRSDLEPEEIVEMMKDADTDWVAKIVPIHRTVRADLDVMKRTATILARRKLDENTSFAVRCRKRGQPPFGQREVEVEVGAAVQEATGAPVDLENPDYYVWIEVLQDTAGISVVPPDLIVSKEVKRVRKWSPGMRPISRAQLKLRELLKRHPYIVREGSDVIDLGAAPGGWSYELARRVEPGTVYAVDPGDLHPKVLKLDNVVHLKKRAEELTEDDIEGRIRLVTSDLNRIHTEATEVTLSVADEFLEPGGFIVQTVKLAVDPSTGEYAAPDVETAVSEVELEYERRGYEILAIERLKRNTPNERTLVARKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH4</accession>
    <name>Q8TYH4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01540">
        <property type="protein sequence ID" value="AAM01540"/>
        <property type="gene ID" value="MK0325"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0325"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="126" mass="14742" checksum="5EDE83690A6E2E58" modified="2002-06-01" version="1">MYRTSSPGREEVLELARQLMRNLRSVLKTLLTEVSDLPYKKARRLLARGLALTFDARPSESPRIRDLLEELPNRLEPFLVRTLEGWPAHYSAHLKRIIRRRKSLDGKHETPGVKLELERWKLGHVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ1</accession>
    <name>Q8TYJ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01523">
        <property type="protein sequence ID" value="AAM01523"/>
        <property type="gene ID" value="MK0306"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0306"/>
    <dbReference type="HOGENOM" id="CLU_1536671_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="174" mass="19014" checksum="44795E9EC8601954" modified="2002-06-01" version="1">MDGSSGLSMCFHLVHEIGRKLDDVEDIVDDAIRLCDEFLQDKLDVDEFKKRLDDMLTRGGGIVGNSGDVKDAAYSIEQAVEATVARVSDLLEKWSREDESKARYERLKDKLEGLRGWVFGGLALSLLAGVLVQLAPQEAKTTLLQALSAITQVLAVVLQLVQVLHGLLPVPISA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL6</accession>
    <name>GSA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">MK0280</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYL6"/>
    <dbReference type="SMR" id="Q8TYL6"/>
    <dbReference type="STRING" id="190192.MK0280"/>
    <dbReference type="EnsemblBacteria" id="AAM01497">
        <property type="protein sequence ID" value="AAM01497"/>
        <property type="gene ID" value="MK0280"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0280"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.283"/>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120483" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="430" mass="47421" checksum="5CD326D03D0EFB4E" modified="2002-06-01" version="1">MGYEDEFPESLELFKRAERVMPGGVSSPVRRFDPYPFYVERAEGSRLYTVDGHVLIDYCLAFGPLILGHAHPEVVEAVVERVREGFHYGTPTLPELKLAEKVVELVPNVEKVRLVNTGTEATMSAIRLARAYTGREKIVKFEGCYHGAHDAVLVRAGSGASELGAPDSPGIPESVAENTLVCPFNDVEAFVETVERFDEEIGAVIVEPVLGNAGCVPPDEEFLKVLREYCDGTERLLIFDEVITGFRLELGGAQEYYGIDADLVCLGKILGGGLPIGAFGGPEEYMSRVAPEGKVYQAGTFNGNPVSATAGLVTLEVLERERPYDELSSKAERLASALEDGLEDRGIEGVVNRVESMFQVYFGIEEVRDYADVNSADHDAFKRFHRELLEHGVWIAASNYEAWFLSIAHTETDLERTEEAFEEALDRLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL7</accession>
    <name>Q8TYL7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein, family MK-41 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01496">
        <property type="protein sequence ID" value="AAM01496"/>
        <property type="gene ID" value="MK0279"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0279"/>
    <dbReference type="HOGENOM" id="CLU_404730_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="679" mass="74494" checksum="0878C6BE8BBF3977" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM1</accession>
    <name>CIMA_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Putative (R)-citramalate synthase CimA</fullName>
            <ecNumber>2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cimA</name>
        <name type="ordered locus">MK0275</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYM1"/>
    <dbReference type="SMR" id="Q8TYM1"/>
    <dbReference type="STRING" id="190192.MK0275"/>
    <dbReference type="EnsemblBacteria" id="AAM01492">
        <property type="protein sequence ID" value="AAM01492"/>
        <property type="gene ID" value="MK0275"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0275"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.278"/>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="SNMFAHE"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01028">
        <property type="entry name" value="CimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024890">
        <property type="entry name" value="Citramalate_synthase_CimA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140413" description="Putative (R)-citramalate synthase CimA">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="14"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="509" mass="55687" checksum="0FC96764B11D80BF" modified="2002-06-01" version="1">MREANADADPPDEVRIFDTTLRDGEQTPGVALTPEEKLRIARKLDEIGVDTIEAGFAAASEGELKAIRRIAREELDAEVCSMARMVKGDVDAAVEAEADAVHIVVPTSEVHVKKKLRMDREEVLERAREVVEYARDHGLTVEISTEDGTRTELEYLYEVFDACLEAGAERLGYNDTVGVMAPEGMFLAVKKLRERVGEDVILSVHCHDDFGMATANTVAAVRAGARQVHVTVNGIGERAGNAALEEVVVVLEELYGVDTGIRTERLTELSKLVERLTGVRVPPNKAVVGENAFTHESGIHADGILKDESTYEPIPPEKVGHERRFVLGKHVGTSVIRKKLKQMGVDVDDEQLLEILRRLKRLGDRGKRITEADLRAIAEDVLGRPAERDIEVEDFTTVTGKRTIPTASIVVKIDGTRKEAASTGVGPVDATIKALERALKDQGIDFELVEYRAEALTGGTDAITHVDVKLRDPETGDIVHSGSSREDIVVASLEAFIDGINSLMARKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ5</accession>
    <name>Q8TYQ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01454">
        <property type="protein sequence ID" value="AAM01454"/>
        <property type="gene ID" value="MK0237"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0237"/>
    <dbReference type="HOGENOM" id="CLU_441895_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="618" mass="68155" checksum="168B43872D50BBEB" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-01-20" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR3</accession>
    <name>SPSS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-phospho-L-seryl-tRNA:Cys-tRNA synthase</fullName>
            <ecNumber evidence="1">2.5.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sep-tRNA:Cys-tRNA synthase</fullName>
            <shortName evidence="1">SepCysS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0229</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25686"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="EC" id="2.5.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with SepRS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SepCysS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.73" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYR3"/>
    <dbReference type="SMR" id="Q8TYR3"/>
    <dbReference type="STRING" id="190192.MK0229"/>
    <dbReference type="EnsemblBacteria" id="AAM01446">
        <property type="protein sequence ID" value="AAM01446"/>
        <property type="gene ID" value="MK0229"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0229"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.230"/>
    <dbReference type="HOGENOM" id="CLU_060476_0_0_2"/>
    <dbReference type="OMA" id="ASGHKSW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043766">
        <property type="term" value="F:Sep-tRNA:Cys-tRNA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01675">
        <property type="entry name" value="Sep_Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013375">
        <property type="entry name" value="Sep_Cys-tRNA_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF3">
        <property type="entry name" value="PTHR43586:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02539">
        <property type="entry name" value="SepCysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000359456" description="O-phospho-L-seryl-tRNA:Cys-tRNA synthase">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="84"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01675"/>
        </source>
    </evidence>
    <sequence length="392" mass="44252" checksum="28CFB3206D0621BA" modified="2002-06-01" version="1">MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKPPIRQFHEDLAEFLGMDVVRITAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAGATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALALLTHVDSEYGNLADAEKFVKICRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLRGSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKEMERIEGVRQLGERPKRHDLVKFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTETIKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR7</accession>
    <name>Q8TYR7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01442">
        <property type="protein sequence ID" value="AAM01442"/>
        <property type="gene ID" value="MK0225"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0225"/>
    <dbReference type="HOGENOM" id="CLU_085612_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="27729" checksum="C2714D537DBC13E0" modified="2002-06-01" version="1">MSTDEWLRELERALKRAEDTDMILNPALGIGVVVLGPLASASVSVIATVCVATASGASVVSAGGTALTVGTVVLFAAFLAVNAALFYILIRRRNKHFERSSELFRIVSEILYRTECISKSCYLAIFRKVKDIEELGHLDEVLWIFLGTITFGVAYLYVAYRLMRDFYLHEARESAIASLLADELDMCISFFRKIPYRNFGIHILIVVFFPILYPYLVYILIKDPNNHFLEHRRFERTLVEKLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU0</accession>
    <name>Q8TYU0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mra1</name>
        <name evidence="5" type="synonym">nep1</name>
        <name evidence="6" type="ordered locus">MK0202</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a pseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in 16S/18S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11633"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0202"/>
    <dbReference type="EnsemblBacteria" id="AAM01419">
        <property type="protein sequence ID" value="AAM01419"/>
        <property type="gene ID" value="MK0202"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0202"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.203"/>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="OMA" id="VYVHTRN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="PTHR12636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="205"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="5">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="5">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="231" mass="26201" checksum="FE58AB69BE193227" modified="2002-06-01" version="1">MPRLCFVLAESELEFIPPKLRGHQHVVRWAKRRGKKPGECLLIASKHHVAMRDKRLPERDRRGRPDIVHVTLLHVLDSPASRENALDVYVHTRHDRVIWFRGDVRLPRDQYRFIGLMEQVLKEGQAPPDSDEPLIEVLDVSVWDVLEANEVNVLLSERGDLIEPVGYMAGLLDAGVERIGVVVGGFPKGDFSEEFYDRADDVVRIYDEPLDAWTVAARIVTAFELAAGILG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV3</accession>
    <name>ATGT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="1">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgtA</name>
        <name type="ordered locus">MK0188</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-cyano-7-deazaguanine + guanosine(15) in tRNA = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYV3"/>
    <dbReference type="SMR" id="Q8TYV3"/>
    <dbReference type="STRING" id="190192.MK0188"/>
    <dbReference type="EnsemblBacteria" id="AAM01405">
        <property type="protein sequence ID" value="AAM01405"/>
        <property type="gene ID" value="MK0188"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0188"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.188"/>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="OMA" id="FPCSCPV"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000247872" description="tRNA-guanine(15) transglycosylase">
        <location>
            <begin position="1"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <sequence length="459" mass="51749" checksum="EF5B6DEFA448B5AF" modified="2002-06-01" version="1">MNVAFEVKDRDVAGRLGRLEVNGRRLKTPALLPVVNPNKPTLDPREISKLGFDGVITNAYIIRKHEHLREQALEEGVHGLLGFDGFVMTDSGSFQLAEYGDVEVSNEEIVRFQAKIGSDVGTILDVPTPPDAPRSRVERDLETTLKRAREAVELDEHPPLALTVQGSTYEDLRRLCAEKLAELPAAVYPVGGVVPLLEEYRFVDVVRVVLAAKSSLPPHRPVHLFGCGHPLAIPLAVAMGCDLFDSASYAIYARSDRYMSILGTLKLEELETFPCSCPACTRHDPDDVREMEPRERTRVLATHNLYELRRVIETTRQAIVSGELWELAESVCRAHPRAWAGMVELARRGGELERWCPAVKRSVFVCDEVSKGRPELRLYRRRLRDRFGELSGRKVVKGISRPYAEIVEWLEPWELAFADEWLGVVPGELSWSYPCHCLVEPSGDDEGEDRRRGEEGRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW0</accession>
    <name>Q8TYW0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type cobalt transport system, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiQ</name>
        <name evidence="6" type="ordered locus">MK0181</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01398">
        <property type="protein sequence ID" value="AAM01398"/>
        <property type="gene ID" value="MK0181"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0181"/>
    <dbReference type="HOGENOM" id="CLU_1202630_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="230" mass="25481" checksum="0E33D05169848E57" modified="2002-06-01" version="1">MRFTEALEWSLTAETVLHRIHPWSKLVGLMTTIFASMLLYDPESIAILSIPYLIGCILARVPVRILARLSIPPMAFLGAVLLLMLPSGVPGREVLLYAVRGATDLLAVLVTTLTTPFNALWSALVVVFPPTLAETGLIFHRSVYRAFEELEGTLNAIRIRGWRLRSINVLGSVIATLLIRSHRSAELVQVSVEVRGATGRVRPLKRFEFTRIDFGWLTMVFCSVALSGVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX0</accession>
    <name>HTPX_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX</name>
        <name type="ordered locus">MK0171</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYX0"/>
    <dbReference type="STRING" id="190192.MK0171"/>
    <dbReference type="EnsemblBacteria" id="AAM01388">
        <property type="protein sequence ID" value="AAM01388"/>
        <property type="gene ID" value="MK0171"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0171"/>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_2"/>
    <dbReference type="OMA" id="AVCCTEG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138918" description="Protease HtpX homolog">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <sequence length="318" mass="34304" checksum="CB6947EB9B97727D" modified="2002-06-01" version="1">MLEAAGAVLLGSAALLMVGRLGNRGFFLWLRTVGILGLLVGILSLALAALTGSAIAGLVVGVITAAMMYLFSSRIVRIQMGAVDAEEFLRYKPEYADKLRRVQEMVSKLASKAGLPEPELVVVPEETGVGGYPNAFATGRRSKPTVGVTEGLLRHLDDDEIYGVLGHELAHVKNRDTLVMTVAAAVSTAIAYAFDPWLNAMYTEDWEDIAFLVLAGMLASLISTLLVAAISRSREYLADEEGAKLSGNPMALAEALEKIEAIVKSNPAPARSLSEVSTAHLWIENPFRGGLLRLFSTHPPVEKRVERLRRLARELQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX7</accession>
    <name>Q8TYX7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small, Ras-like GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0164"/>
    <dbReference type="EnsemblBacteria" id="AAM01381">
        <property type="protein sequence ID" value="AAM01381"/>
        <property type="gene ID" value="MK0164"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0164"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.164"/>
    <dbReference type="HOGENOM" id="CLU_1399745_0_0_2"/>
    <dbReference type="OMA" id="ATKIDFH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="22"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="208" mass="22818" checksum="B542253F0F19F539" modified="2002-06-01" version="1">MGVREVLRRLAAALRSGPEELDIGIYGAPNVGKTTLANRIAQDWEAEEFGQVSEVPHETRESVRREVAIEVGSTTVKFNIVDTPGIATKVDYRKFLEYGLDVQEAKQRAKEATRGVVEAIKLLKDIDGALVVIDSTKDPLSQVNVTLIGNLEANDVPFLVVANKIDLEEADPEAVRKAFSEYPVVAVSAKTGENMAKLYEAMVREFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ4</accession>
    <name>Q8TYZ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01364">
        <property type="protein sequence ID" value="AAM01364"/>
        <property type="gene ID" value="MK0147"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0147"/>
    <dbReference type="HOGENOM" id="CLU_1113868_0_0_2"/>
    <dbReference type="OMA" id="LDEHYTD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="236" mass="25642" checksum="D6FA734F1ED27581" modified="2002-06-01" version="1">MTRLLGGPGLTPRIMVGTSPFLGAGQFGHRALLYRRTFYHRPENIVALLEYCAEELGVTGVQALADPVIIGALRDADPDLDVVAVVGLRNLEEELEMLENLNLRAVLLHASCVDGEDVGEVSAKLDEIRSRLDVPVGIATHRPDETLPRWEDKKTADVYMVPLNPVGAFMGDQKAVEELLAETDRTVIAKKVLAAGSLPPEEGLPYAARYADAVAVGITGKKEAEETLRIAKRYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ01</accession>
    <name>Q8TZ01_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01357">
        <property type="protein sequence ID" value="AAM01357"/>
        <property type="gene ID" value="MK0140"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0140"/>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="OMA" id="IERSHDA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="24584" checksum="BD61B1C0D6557C31" modified="2002-06-01" version="1">MRVLFLGYRYGSRAAENVGSRSDFDVEFLKVKEPPENVILDEEYARTLLPPSYSGFDLVISYLQHPDLQLALAEVCDSPILYGITPDPAVREKIERSHDAVAFPETTMCSLLPDTGIPEVDRFAERFGRPKLEVSVSGGKIRTVRVVRGAPCGATWVAAERVEGMSVDEEAVNAFALAACHHCVAPRFGKFESKDVTAYLHGVALAEALGIELDVDLEGFELPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ16</accession>
    <name>Q8TZ16_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Zn-ribbon containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0125"/>
    <dbReference type="EnsemblBacteria" id="AAM01342">
        <property type="protein sequence ID" value="AAM01342"/>
        <property type="gene ID" value="MK0125"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0125"/>
    <dbReference type="HOGENOM" id="CLU_187343_0_0_2"/>
    <dbReference type="OMA" id="KCARNLG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_S26e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_S26e_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="10209" checksum="CD870BF2CF23DA87" modified="2002-06-01" version="1">MARNPRRKGRDGWKQSKSRGKPSFVTCECCGGKVPRHKAIPWTQGFRITDPVVRQAVDDRYVHTFSRKVYYCPKCARFLGIRKPKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ19</accession>
    <name>GLYA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1">2.1.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">MK0122</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of serine and glycine with tetrahydromethanopterin (H4MPT) serving as the one-carbon carrier. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methylenetetrahydromethanopterin + glycine + H2O = 5,6,7,8-tetrahydromethanopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:47104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ19"/>
    <dbReference type="SMR" id="Q8TZ19"/>
    <dbReference type="STRING" id="190192.MK0122"/>
    <dbReference type="EnsemblBacteria" id="AAM01339">
        <property type="protein sequence ID" value="AAM01339"/>
        <property type="gene ID" value="MK0122"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0122"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.121"/>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113714" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00051"/>
        </source>
    </evidence>
    <sequence length="428" mass="46897" checksum="C2C9656705115F6B" modified="2002-06-01" version="1">MFGLEDVHSVVRAVEKHHEWLKKCLPMIASENVTSPAVREMLVTDFGHRYAEGKPGERLYEGCEYIDEVELACVRLAKELFGAEHANVQPTSGVVANLAALFALTEPGDTILGLRISHGGHISHHDISAPGVRGLNVEYLPFDEEDMAIDVDGMVRKIEEVEPSVVMLGASLFLFPHPVEEAVEAVEAVGGYVVYDAAHVLGLIAGGQFQDPIREGAHVVTGSTHKTFPGPQGGIVLCQRDLADDIDEAVFPGLVSNHHLHHVAALAVALAEFKEYGERYARDTVRNAKALAEALYAEGLRVLCEHRGFTESHQIAVDVREQGGGAVIAEKLESANILCNKNLLPWDDESKSHDPSGIRLGTQELTRLGMGLSEMEYIAELIADVVLGRREPSEVRKDVEELRREFQEVKYGFGSGVGAHEWPRLADW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ26</accession>
    <name>Q8TZ26_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATP-utilizing enzymes of the ATP-grasp superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0114</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0114"/>
    <dbReference type="DNASU" id="1477417"/>
    <dbReference type="EnsemblBacteria" id="AAM01331">
        <property type="protein sequence ID" value="AAM01331"/>
        <property type="gene ID" value="MK0114"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0114"/>
    <dbReference type="HOGENOM" id="CLU_059501_1_0_2"/>
    <dbReference type="OMA" id="DVNPRPT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024710">
        <property type="entry name" value="MfnD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016766">
        <property type="entry name" value="UCP016766_ATPgrasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="201"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="307" mass="33860" checksum="FAA264CE4612E376" modified="2002-06-01" version="1">MRVYIYEYAVATGDEEFLAEGRAMLESLLRAFAKSGYETLTVAHPSVGVRWADEVLRDETQALECADLTLVIAPESDGLLESKVREYSRETEVIGPTPRAIRVAADKRKTEDALRDARSFQLPTREADVMVSKPADGAGSEGVRIGRGELSRELIPGSHHSLLCVSDGETVDVLGINDQFVAFAGRELVYLGGRTPSDHRELTRIARDIAEEVVERIPGLVGLFGVDLVMKGGEPYLIEVNPRPTTPTVAAALEHPEAVVRSLLEGPTGKVLRYRREYVYVKRGAEALVPEKFEVVEDFHGLRVYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ30</accession>
    <name>Q8TZ30_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartokinase</fullName>
            <ecNumber evidence="7">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">lysC</name>
        <name evidence="10" type="ordered locus">MK0109</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0109"/>
    <dbReference type="EnsemblBacteria" id="AAM01326">
        <property type="protein sequence ID" value="AAM01326"/>
        <property type="gene ID" value="MK0109"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0109"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.108"/>
    <dbReference type="HOGENOM" id="CLU_009116_6_0_2"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04244">
        <property type="entry name" value="AAK_AK-LysC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041746">
        <property type="entry name" value="AK-LysC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="325"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="403"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="467" mass="49821" checksum="4913B23A07EB2F6F" modified="2002-06-01" version="1">MRVVMKFGGTSVGTGESIRKVAKIVTDAAEEHEVIVVVSAMSGVTDELVRAAESAPDWTEEDVKNFVGKLRRRHGKAASEAISSDLIRREVMGYVDSLLEELEKVLLGLSYVGEVTPRSMDLILSFGERMSAPIVAGALRDRGLEAEHLEGGEAGVITDDGFGEAEPILPACRRKAQKTLIPMIESGKIPVITGFIGRTIDGEVTTLGRGGSDYSAAIIGCISEADEVQIWTDVDGVMTANPNLVPDARTVPRLSYEEAMELASFGAEVLHPKTVIPARSENIPIRVKNTFNPESEGTLITSESEPSEQVVKAVASSSDVGMIDIRGTTMIGRPGVAGRIFSRLGDEGINVIMISQSASESNISIVVSRPEVRRAARIIEREFVGERVVERVTTYEDVAVVAVVGEGMRGTPGVASRVFRAVADAGVNIKTISQGASEVNISFVVAEEDEAAAVNAVHSEFELGEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ58</accession>
    <name>Q8TZ58_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate:ferredoxin oxidoreductase, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">porD</name>
        <name evidence="4" type="ordered locus">MK0081</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0081"/>
    <dbReference type="EnsemblBacteria" id="AAM01298">
        <property type="protein sequence ID" value="AAM01298"/>
        <property type="gene ID" value="MK0081"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0081"/>
    <dbReference type="HOGENOM" id="CLU_139698_1_1_2"/>
    <dbReference type="OMA" id="GSWRTFK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PTHR43724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="89" mass="9970" checksum="F307311175CBABE0" modified="2002-06-01" version="1">MGNEEFTIGAVVREPGSTKRNKTGRWRVFRPVLDQEKCMNCGLCFMYCPDGCIRPSDDGYVIDYDYCKGCGICESVCPVNAIEMVLEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ63</accession>
    <name>Q8TZ63_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-33 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0076</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01293">
        <property type="protein sequence ID" value="AAM01293"/>
        <property type="gene ID" value="MK0076"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0076"/>
    <dbReference type="HOGENOM" id="CLU_1017847_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="275" mass="30221" checksum="A8ED0045417534FD" modified="2002-06-01" version="1">MIDISSLSWAVALGVVRGLYVFAGSFIAAAVYRYVAEERIRMTTSAFMGLLTAGFAAGPKELTALTYQNPNVEMIAWAIATLFAIPARTYGDAIGERILRARIRASMNPRTKVYRLPENPNEIKDIPGEPPAPMEVKERIAGREYEFPRGTPKEEVERVIKRDLESETGIGRAVVRVRNGDVEVLVAGAKPPVSHTLPPDKVAVSVEPLGGAIHIGEGDRVRVFVDGRELGEAEVWRRVDDRVVLVMEERTAEELLKEITQGKQVSLMAVRGEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ67</accession>
    <name>Q8TZ67_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-21 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01289">
        <property type="protein sequence ID" value="AAM01289"/>
        <property type="gene ID" value="MK0072"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0072"/>
    <dbReference type="HOGENOM" id="CLU_709058_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="389" mass="43433" checksum="0EB1F65B0CA8F81C" modified="2002-06-01" version="1">MIGRIFRRESKVVRDRLMKIRQTIDATEVDPTAVAETIGGPMESSSRSTEEGSEKARQDEVEEEPSRESHEESSAEEHSGVSAEAFLEMYSTRKGTAAVVAYPLYEICKTLCLAGILRRKTRKILEDLTAVRLPLLLASHNAVGPTSDLLEGFPPKASGYQVASPYSADALRSLAPESIAVMGYKSGHVGAEPLRGWKRMPNVCSWGVEAAVADELWDQAPVAVAVTDTISALYGVYLSSMLDAPALDFSYLWKFTKGKPLWRDPRGGKHAIQWTQWKFLRELKLDYLIVVSCLKDRRFKRVVRTLESEIKVKIVPIVSEDPRELGELLRTTLENQVPMVGGEVHRTFQVEPVLVEFVKRAILAPLPVSDFNEAKSSRFYPLIIDISIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ68</accession>
    <name>Q8TZ68_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-utilizing enzymes of the PP-loop superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0071"/>
    <dbReference type="EnsemblBacteria" id="AAM01288">
        <property type="protein sequence ID" value="AAM01288"/>
        <property type="gene ID" value="MK0071"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0071"/>
    <dbReference type="HOGENOM" id="CLU_1096716_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="PTHR43169:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="253" mass="28360" checksum="71A806BAB9BD93BA" modified="2002-06-01" version="1">MRPFLSRLRALISYIKKTYDRVAVAYSGGTDSSVLLYAAVKALGRKNVVACTACLPYVVNVCPPTPMDVKQIRIRPDVEDVLREGRDEHPCYLCKRAIYEAIHETIGGVDGVLDGTNVSELTRGRPGLRALYELDVDVPMIKCGLGDQTTSVLAAYLNLRARSGACSLVLSPEWPDVEVNDPGKLNRTARLRKPQSERWSLSECLEFTIHAPLPERRRERYECVRRGDALVFRVLSGKVTEALASYFRECWNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ84</accession>
    <name>Q8TZ84_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ordered locus">MK0055</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0055"/>
    <dbReference type="EnsemblBacteria" id="AAM01272">
        <property type="protein sequence ID" value="AAM01272"/>
        <property type="gene ID" value="MK0055"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0055"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.56"/>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
    <dbReference type="OMA" id="HRCLQMT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="PTHR13930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="46"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="34878" checksum="DE55CB7AD2FFC02E" modified="2002-06-01" version="1">MLHADVLKVLRKQGYGLVGKHSAVKPCHWCREAIKNGRHCYKAKFYGVESHRCLQMTPTVAWCQQRCVYCWRPVELTVGTHDVPDPDDPDLIVEESVEQQRRFLQGYGHLEGEARKRWKESLEPRHAAISLAGEPTLYPRIGELIDAFHSHGFDTTFLVTNGLRPDRVEELEREPTQLYVSLDSPNEELHRQINRPTIPDSWDRIMRTLELLNSLSCRTVIRITAIKGWNMEGTAEEFGELLADVEPDYVEVKAFMCVGWAAFRMSPDNMPSHEEVRKFAAEIAEHAGFELVDESPPSRVALLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ95</accession>
    <name>Q8TZ95_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0041"/>
    <dbReference type="EnsemblBacteria" id="AAM01258">
        <property type="protein sequence ID" value="AAM01258"/>
        <property type="gene ID" value="MK0041"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0041"/>
    <dbReference type="HOGENOM" id="CLU_450283_0_0_2"/>
    <dbReference type="OMA" id="VPIAEWV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007548">
        <property type="entry name" value="DUF505"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04458">
        <property type="entry name" value="DUF505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029056">
        <property type="entry name" value="DUF505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="606" mass="67483" checksum="D83D96AEC3FE6176" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ97</accession>
    <name>Y039_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0273 protein MK0039</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0039</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ97"/>
    <dbReference type="SMR" id="Q8TZ97"/>
    <dbReference type="STRING" id="190192.MK0039"/>
    <dbReference type="EnsemblBacteria" id="AAM01256">
        <property type="protein sequence ID" value="AAM01256"/>
        <property type="gene ID" value="MK0039"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0039"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.39"/>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="OMA" id="FSQQFLW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184586" description="UPF0273 protein MK0039">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="1">
        <location>
            <begin position="4"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <sequence length="252" mass="27940" checksum="09A90AD595F6BBC0" modified="2002-06-01" version="1">MAVERVSTGIPGMDEVLNGGIPERNAVLLTGGPGTGKTIFSQQFIWAGLEEGEPGVFVTLEEHPVQVRKNVEGFGWNFREYEEEGLLAVVDAFTGGIGRASEYEKYVVKDPTDASELIGVIRQAVNDVEAKRVAIDSVTPLYIDKPSVARRIMFRLKRMLAGLGCTSILVNQIAAHERGFGGPGVEHAVDGIIRLDLDEVEGRLWRSLIVWKMRGTAHSMRRHPFEITDEGIRVDPEKVFVKERGEVREVED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-06" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA6</accession>
    <name>RS5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps5</name>
        <name type="synonym">rpsE</name>
        <name type="ordered locus">MK0029</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZA6"/>
    <dbReference type="SMR" id="Q8TZA6"/>
    <dbReference type="STRING" id="190192.MK0029"/>
    <dbReference type="PRIDE" id="Q8TZA6"/>
    <dbReference type="EnsemblBacteria" id="AAM01246">
        <property type="protein sequence ID" value="AAM01246"/>
        <property type="gene ID" value="MK0029"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0029"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.29"/>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_S5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_S5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131649" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="51"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <sequence length="226" mass="24835" checksum="F6824414024079B8" modified="2002-06-01" version="1">MSVPPHLEEWEPKTKLGRMVKEGEIKTIDEVFAQGWKILEPEIVDWLLPDLEEEVLDVNMVQKMHKSGRRVRFRVTVVVGNKDGFVGVGQGKAREVGPAIRAAIDRAKLNIIKVRRGCGSWECGCGRPHSIPFEITGKCGSVRITLKPAPRGTGLVAGETAQKVLEMAGIEDVWTKTTGGKGKGETRTTINFAKATFDALRNLIYVRMREEEAERLGIVSGSAEGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA8</accession>
    <name>RL15_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15</name>
        <name type="ordered locus">MK0027</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZA8"/>
    <dbReference type="SMR" id="Q8TZA8"/>
    <dbReference type="STRING" id="190192.MK0027"/>
    <dbReference type="EnsemblBacteria" id="AAM01244">
        <property type="protein sequence ID" value="AAM01244"/>
        <property type="gene ID" value="MK0027"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0027"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.27"/>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="OMA" id="WGRVGQH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_prot_L15/27a_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251594" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="154" mass="17040" checksum="24364D7588EE902A" modified="2002-06-01" version="1">MVVRRKKKSPKKYRGSRTHGGGSHKNRRGAGNRGGRGMAGSHKHKWFHVIKYMPDHFGKRGFNRPPKVVREPNTINVGELDALADKLLEDGIAEKDGDKIVIDVTDERLKPYGGPFDKVLGGGHVKRPLVVVAPEFTERAVEKLEEAGGEAREA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB7</accession>
    <name>Q8TZB7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0018"/>
    <dbReference type="EnsemblBacteria" id="AAM01235">
        <property type="protein sequence ID" value="AAM01235"/>
        <property type="gene ID" value="MK0018"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0018"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.17"/>
    <dbReference type="HOGENOM" id="CLU_1691544_0_0_2"/>
    <dbReference type="OMA" id="SMLVIMD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010254">
        <property type="entry name" value="B12-dep_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012019">
        <property type="entry name" value="HcgB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11576">
        <property type="entry name" value="HcgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005018">
        <property type="entry name" value="UCP005018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="160" mass="17897" checksum="9E10FF23C8F2EFE5" modified="2002-06-01" version="1">MLKETLLKAWWESWEGTRRGDEEREVEGLREYLTSADRLAVVTGNEDKLRAVNKVLRRFGLPEAEMIRVPTEMADATPCPAIFKAIMGVQVSDADVVIARGRLGVPGSGAMTVFMDARCRLLTAALSPPHVLHEMSVKEAMEREAEEALRRLGMRETTSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC9</accession>
    <name>RTCA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="1">RNA cyclase</shortName>
            <shortName evidence="1">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="1">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rtcA</name>
        <name type="ordered locus">MK0001</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZC9"/>
    <dbReference type="SMR" id="Q8TZC9"/>
    <dbReference type="STRING" id="190192.MK0001"/>
    <dbReference type="EnsemblBacteria" id="AAM01218">
        <property type="protein sequence ID" value="AAM01218"/>
        <property type="gene ID" value="MK0001"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0001"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1"/>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="OMA" id="PKPGLSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="PTHR11096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="PTHR11096:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="SSF52913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156427" description="RNA 3'-terminal phosphate cyclase">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <sequence length="352" mass="38092" checksum="9E0952828B031E5A" modified="2002-06-01" version="1">MSLIEIDGSYGEGGGQILRTAVGMSALTGEPVRIYNIRANRPRPGLSHQHLHAVKAVAEICDAECEGLEIGSTEIVFEPGKVKGGEYEVDIGTAGSVTLLLQAVKLAAIAADGPVEMEVRGGTDVKWSPPVDYEINVNAHYLDRLGYRYELEVLRRGHYPRGGGIVRARMEPPKRLKPLEAVKFGELESVRGISHCVRLPPHVAERQAKAASEIIERELGIRPEIEIETYPKGRDPHLGPGSGIVLWAEDDQGNRIGADALGEKGKPAEVVGREAAEQLVQRLRTGMALDEHMGDQILPFLAIADGESVFGVTGVDPHLPTNAWVVEKFLPVSVEIRGKEGEPATVEVRPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2021-06-02" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVK3</accession>
    <name>F1SVK3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02077">
        <property type="protein sequence ID" value="AAM02077"/>
        <property type="gene ID" value="MK0864"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0864"/>
    <dbReference type="HOGENOM" id="CLU_2613656_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="78" mass="8830" checksum="56DC7214C797BFB2" modified="2011-05-03" version="1">MARKARVTLTLSEDALARVLATKDALEGDSISATVGKLVKFGFAYLREKFPELFEGINLEEYREKARERWFDSGSSRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>P94951</accession>
    <accession>Q9UWL4</accession>
    <name>MTD_METKA</name>
    <protein>
        <recommendedName>
            <fullName>F420-dependent methylenetetrahydromethanopterin dehydrogenase</fullName>
            <shortName>MTD</shortName>
            <ecNumber>1.5.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtd</name>
        <name type="ordered locus">MK0011</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="245" first="386" last="391">
            <title>Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri.</title>
            <authorList>
                <person name="Klein A.R."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9151968"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.t01-1-00386.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1993" name="Arch. Microbiol." volume="160" first="186" last="192">
            <title>Two N5,N10-methylenetetrahydromethanopterin dehydrogenases in the extreme thermophile Methanopyrus kandleri: characterization of the coenzyme F420-dependent enzyme.</title>
            <authorList>
                <person name="Klein A.R."/>
                <person name="Koch J."/>
                <person name="Stetter K.O."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="8215796"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-35</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Acta Crystallogr. D" volume="59" first="1653" last="1655">
            <title>Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase from Methanopyrus kandleri: the selenomethionine-labelled and non-labelled enzyme crystallized in two different forms.</title>
            <authorList>
                <person name="Hagemeier C.H."/>
                <person name="Shima S."/>
                <person name="Warkentin E."/>
                <person name="Thauer R.K."/>
                <person name="Ermler U."/>
            </authorList>
            <dbReference type="PubMed" id="12925803"/>
            <dbReference type="DOI" id="10.1107/S0907444903014896"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2003" name="J. Mol. Biol." volume="332" first="1047" last="1057">
            <title>Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) from Methanopyrus kandleri: a methanogenic enzyme with an unusual quarternary structure.</title>
            <authorList>
                <person name="Hagemeier C.H."/>
                <person name="Shima S."/>
                <person name="Thauer R.K."/>
                <person name="Bourenkov G."/>
                <person name="Bartunik H.D."/>
                <person name="Ermler U."/>
            </authorList>
            <dbReference type="PubMed" id="14499608"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(03)00949-5"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:16721"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Strictly dependent on the presence of salts for activity.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Optimum temperature is 75 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text>Homohexamer composed of a trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MTD family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.1"/>
    <dbReference type="EMBL" id="Y10251">
        <property type="protein sequence ID" value="CAA71298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="1QV9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.54 A"/>
        <property type="chains" value="A/B/C=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="1U6I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="1U6J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="1U6K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.55 A"/>
        <property type="chains" value="A/B/C=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="3IQE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="3IQF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-283"/>
    </dbReference>
    <dbReference type="PDB" id="3IQZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-283"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QV9"/>
    <dbReference type="PDBsum" id="1U6I"/>
    <dbReference type="PDBsum" id="1U6J"/>
    <dbReference type="PDBsum" id="1U6K"/>
    <dbReference type="PDBsum" id="3IQE"/>
    <dbReference type="PDBsum" id="3IQF"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <keyword id="KW-0002">3D-structure</keyword>
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    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000075039" description="F420-dependent methylenetetrahydromethanopterin dehydrogenase">
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        </location>
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        </location>
    </feature>
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            <end position="283"/>
        </location>
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    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="4" ref="3">
        <original>C</original>
        <variation>E</variation>
        <location>
            <position position="11"/>
        </location>
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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            <end position="63"/>
        </location>
    </feature>
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        <source>
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        </source>
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        </source>
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        <source>
            <dbReference type="PDB" id="1QV9"/>
        </source>
    </evidence>
    <sequence length="283" mass="31382" checksum="D2B778CB34D3034C" modified="2007-01-23" version="3">MTVAKAIFIKCGNLGTSMMMDMLLDERADREDVEFRVVGTSVKMDPECVEAAVEMALDIAEDFEPDFIVYGGPNPAAPGPSKAREMLADSEYPAVIIGDAPGLKVKDEMEEQGLGYILVKPDAMLGARREFLDPVEMAIYNADLMKVLAATGVFRVVQEAFDELIEKAKEDEISENDLPKLVIDRNTLLEREEFENPYAMVKAMAALEIAENVADVSVEGCFVEQDKERYVPIVASAHEMMRKAAELADEARELEKSNDAVLRTPHAPDGKVLSKRKFMEDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
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    <name>Q8TGY2_METKA</name>
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        <submittedName>
            <fullName evidence="7">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
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        <name evidence="7" type="ordered locus">MK1575</name>
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        <lineage>
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            <taxon>Methanopyrales</taxon>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="431" mass="49013" checksum="C1476650F3988F31" modified="2002-06-01" version="1">MEERGHSLKAALRNALRGIGGRRHRFVQALAHELVRVLGNVDLVLNRVLRGSTVEDLHPYLRNALRVGTWEIHWRREHPGPVTKAVVDVVKERVGPKHARFANAILRQVERVNPEEVISSIRDPTARFAARYNFPKWYVELVRSAFDSMDELRKFLEACNRRPARYVRVNTLVSDPEEVVRRLKRRGIEAERDPDVPDVLRIRSAETPVIKTPEFKKGEVYPQTKASAAVAHAAEPEPGMTVVDLCAAPGGKTTHLAQLMEGRGEIIAIDMHPKRFGTLKKRVRQFHADDIVETLCMDARDAPDQLGEGIADLVLVDPPCTGTGSVYSKPEKRWDRETTGEPTKWAQLQWELLKVAVRLLKPGGRIVYSTCSITLTENERLIERLVRRYRGLELVDVPLEWASPGVRMPEARRIWPHRHDTDGFFVARVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ9</accession>
    <name>Q8TGZ9_METKA</name>
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        <submittedName>
            <fullName evidence="3">Distinct Superfamily II helicase family with a unique C-terminal domain including a metal-binding cysteine cluster</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">MK0244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAM01461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAM01461">
        <property type="protein sequence ID" value="AAM01461"/>
        <property type="gene ID" value="MK0244"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0244"/>
    <dbReference type="HOGENOM" id="CLU_406353_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="45"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="230"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="676" mass="77257" checksum="C9B8E3AD008F59A2" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR5</accession>
    <name>Q8TUR5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type phosphate transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pstS</name>
        <name evidence="3" type="ordered locus">MK1690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02902">
        <property type="protein sequence ID" value="AAM02902"/>
        <property type="gene ID" value="MK1690"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1690"/>
    <dbReference type="HOGENOM" id="CLU_026228_5_1_2"/>
    <dbReference type="OMA" id="FKKKALC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="31"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="329" mass="35785" checksum="69EB785FF3177654" modified="2002-06-01" version="1">MGGEILKKYLIAAVVAIAVIAGGAAYYYSTAGAVKTQLRIGGSTSLMPFMMKVAAYVGEEHRMTWKQIGEQVGVQGLPDEPVQLMVSDGGSSAGIKGLIEGTFDIAMASRPLKPEEAQQLSDPVMCPVVLSAIVPIVNGKTLGQLDDIDPKTLRDIYTGKIKYWDQVKPGLPHKKIVVIGRAKGSGTRSTFDKYLNIQDYTKDAILCGSNSEVLEKVEKTPYAIGYVDYAYVVKAKKGGKVPSAVKPLKVNGVEANEQTIIAKKYPLVRAEYLIIDRSNAPKTAIALIKWIRKPDVNKEFAEKVGYVPVPEDAPLFSKKPYIEIKPLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS8</accession>
    <name>Q8TUS8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1675"/>
    <dbReference type="EnsemblBacteria" id="AAM02888">
        <property type="protein sequence ID" value="AAM02888"/>
        <property type="gene ID" value="MK1675"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1675"/>
    <dbReference type="HOGENOM" id="CLU_152996_1_0_2"/>
    <dbReference type="OMA" id="LLICPYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002572">
        <property type="entry name" value="DUF22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01629">
        <property type="entry name" value="DUF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF22" evidence="1">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="126" mass="14523" checksum="7BB3CA34EEDF06F1" modified="2002-06-01" version="1">MVFVSVAKRKVTERVRRRREPYDFKTDMFEGRFEPLIAAEDVTVEEGEDVIIKVEPIEIPPHTMVLLSPYARNPYGHVLAVAEEFPKMMELGRKVEQVYFAAVRHGRIRKGDVLGVLILIELKGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS9</accession>
    <name>VATD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">V-type ATP synthase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">V-ATPase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpD</name>
        <name type="ordered locus">MK1674</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUS9"/>
    <dbReference type="SMR" id="Q8TUS9"/>
    <dbReference type="STRING" id="190192.MK1674"/>
    <dbReference type="EnsemblBacteria" id="AAM02887">
        <property type="protein sequence ID" value="AAM02887"/>
        <property type="gene ID" value="MK1674"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1674"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1838"/>
    <dbReference type="HOGENOM" id="CLU_069688_2_1_2"/>
    <dbReference type="OMA" id="TQKRANA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="PTHR11671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144251" description="V-type ATP synthase subunit D">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <sequence length="232" mass="26560" checksum="D44965B9DEA1505F" modified="2002-06-01" version="1">MTQEILEDVNPTRMELLKLQDRIELAKKGHKLLKEKRDALIMEFFEMVKRASEIREQAVKKLMEAYSKLAAAKVTVGEIGVERASMATGEEIKVDVGSRNVMGVVVPIIERVSEDGGSKVVYGFADTSGALDEAMRAFTEAIDAVLELAEIEETLRLMAEEIERTKRRVNALEHIVIPRLENTEKYIEMKLDEQERENFVRLKRVKDLIERKKLKEELERVVEEGAELPSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT1</accession>
    <name>Q8TUT1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted phosphatidlglycerophosphatase A fused to a uncharacterized conserved domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pgpA</name>
        <name evidence="2" type="ordered locus">MK1672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TUT1"/>
    <dbReference type="STRING" id="190192.MK1672"/>
    <dbReference type="EnsemblBacteria" id="AAM02885">
        <property type="protein sequence ID" value="AAM02885"/>
        <property type="gene ID" value="MK1672"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1672"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1836"/>
    <dbReference type="HOGENOM" id="CLU_833194_0_0_2"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14883"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017577">
        <property type="entry name" value="Ribazole_CobZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021167">
        <property type="entry name" value="UCP027887_Ptd-glyceroPase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027887">
        <property type="entry name" value="UCP027887_Ptd-glyceroPase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03161">
        <property type="entry name" value="ribazole_CobZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PgpA" evidence="1">
        <location>
            <begin position="259"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="357" mass="39236" checksum="9A92FCB193794B48" modified="2002-06-01" version="1">MEFFHVIKDERAVVLEFENPVKYVSSAFYSDGVGEVRWVANIRVEKGWSHDDPWGYVEERLHELGLEPEDTLAFLTAADVEQAAIVQKGDVFAVATAGFGNAYCSKTKEHDLGGPGTVNVIAVVGRPLTTRSLVEALTWAVEAKCHGVLRIVLGNEGPCLGTTTDSVAVLCPQGDELDSFCGPATELGRRLMSAVEEAVVTAGERAGYSPTRSIKTILEEEGIELNDLVEAGRELFVGEWKEEHASRVLEELERGLENPNVALAVFTALLIDRFVRLGTYPEECGELREDPGWVYFDEVLGQFVAMELGGYGALFNFKRYDEEKPGILKEVDERWVMLDDVLAGLVAGAMTAAFRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV1</accession>
    <name>Q8TUV1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">L-lactate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lldP</name>
        <name evidence="8" type="ordered locus">MK1652</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="TCDB" id="2.A.14.1.4">
        <property type="family name" value="the lactate permease (lctp) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02865">
        <property type="protein sequence ID" value="AAM02865"/>
        <property type="gene ID" value="MK1652"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1652"/>
    <dbReference type="HOGENOM" id="CLU_586103_0_0_2"/>
    <dbReference type="OMA" id="MIANQLP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015129">
        <property type="term" value="F:lactate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003804">
        <property type="entry name" value="Lactate_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30003">
        <property type="entry name" value="PTHR30003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02652">
        <property type="entry name" value="Lactate_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="466" mass="49375" checksum="CE7B821ABB3860E9" modified="2002-06-01" version="1">MRLYRRRDPEPQSPSSSEGGLPLPSLALVVPLISISALIVAALRGWGLFRASSLSLALYLIASTFTPFTVQVPYVRVVDSALKGVEILAVVLASLTLVEMMREDGVLEDIVHILKETCQLRGPCLVGLSVVATGAFESVASFGTPATLVGPLLVEAGLSKTTAAAAALVGHAPFGVFAAFGVPILVTSGVSGIDPKKLSFDVALCIAPSLLVLPYAVAETADLRVSKVLLTTMGIMTTASLLAVWWVASPSIVGPLLVLGVASGFLWYLRVAGEEARIKVSNDTIKGMIVYGLVVVGIGVVKALDVKWVQPWMLLWVAVLLYATPRWTRMVGALIRVLRKTWEELLSIVLLMVLGTLIAHSELPSAIRPYAGSPLVTFLVGFMGEMVVGSTTAVMATFASGTPYPEITLAGAACAAAACPYNVAVAAAAVRCHEKRVMRRSLLGSMYLTVPTLAWCTMLWIWGAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV2</accession>
    <name>Q8TUV2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA photolyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">splB</name>
        <name evidence="7" type="ordered locus">MK1651</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1651"/>
    <dbReference type="EnsemblBacteria" id="AAM02864">
        <property type="protein sequence ID" value="AAM02864"/>
        <property type="gene ID" value="MK1651"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1651"/>
    <dbReference type="HOGENOM" id="CLU_062373_0_0_2"/>
    <dbReference type="OMA" id="CGMTIHT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="PTHR43432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="26"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="318" mass="35553" checksum="1F2ECE9CC5F5B6D5" modified="2002-06-01" version="1">MRRELIERLESRLDRREIEKARRDSHARRRPRPCGITVHPGHGCPRACSYCYIPEMGFRFERARPYRLSGEGMVLALLYNRGFEPGREGTFIAVGSVTDPFLPELADKTLEYLRTFSRWLGNPTQFSTKSAIDGEVAESLARLELPLNGLVTILTPDREKASRLEPRAPRPEERLETITELSKAGLTVDLFFRPILPGIVGLEEAEELFRMARDAGARGVVVGGFRVNEGILSRLKRSGFDVSEIVNRANRPIPKGRKQVYVRTGDIKERLLRIAREVGLTPFGAACCACASAAQVPCPNRCWEGPFCTECGNPACPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW2</accession>
    <name>Q8TUW2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ftsJ</name>
        <name evidence="2" type="ordered locus">MK1641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02854">
        <property type="protein sequence ID" value="AAM02854"/>
        <property type="gene ID" value="MK1641"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1641"/>
    <dbReference type="HOGENOM" id="CLU_948676_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FtsJ" evidence="1">
        <location>
            <begin position="130"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="293" mass="32922" checksum="A9098EF34D19B7C9" modified="2002-06-01" version="1">MSELNVVELFHGDLYSIAYRAELDKFLPMIGRARITPAELTLAGLLELGYEVEVTTLEGKSLPGRRRIELADEVKGVVTGRYVEELLDSDRVHHLRLSARCLGAEVKIRYSSDWKVEIGGYEVDPSKLHKNRALVKLLEFVSTREPSFTDLVVDLGAAPGGWSSFAAQMAENVVAVDPARLEDRVRELENVHHLRITAHEFVLPEMIGVGFPGEKVTLLSDVYSGNPEDDLYAVLRLLERLENDVVWGFVKVAPAEDDVLEWFMEEIEEAGFAVENVNLESASSNETFVYFRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV23</accession>
    <name>RS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps2</name>
        <name type="ordered locus">MK1578</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV23"/>
    <dbReference type="SMR" id="Q8TV23"/>
    <dbReference type="STRING" id="190192.MK1578"/>
    <dbReference type="EnsemblBacteria" id="AAM02791">
        <property type="protein sequence ID" value="AAM02791"/>
        <property type="gene ID" value="MK1578"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1578"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1739"/>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="OMA" id="QQKTKDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_S2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_S2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_S2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_S2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_S2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="PTHR11489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000134324" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00291"/>
        </source>
    </evidence>
    <sequence length="203" mass="22822" checksum="94D3F262E150D72E" modified="2002-06-01" version="1">MSENDDLLVPLNDYLAAGVHIGTQQKTKDMEPFIYRTRADGLHVIDVRKTDERIRIAANFLSMYNTDEILVVSRRYYGQKPVSKFAEATGTTAIPGRFVPGTLTNPEYDGYLEPEVIVLTDPRADFQALVEAQSVGIPIVALCDTDNFTGNVDLAIPTNNKGRKALALVYWLLARELLKKLGRLEEDEEFEYDPEDFEGPPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV26</accession>
    <name>Q8TV26_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase of the beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1574"/>
    <dbReference type="EnsemblBacteria" id="AAM02787">
        <property type="protein sequence ID" value="AAM02787"/>
        <property type="gene ID" value="MK1574"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1574"/>
    <dbReference type="HOGENOM" id="CLU_036012_1_0_2"/>
    <dbReference type="OMA" id="VISHEHW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="23"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="22373" checksum="021EE179A456BE8C" modified="2002-06-01" version="1">MVEITIVVDNRVGMVRDPYVARHGLAIIVETDSSLILFDTGPSANTLANNLRLAGFDASFDHVVISHEHWDHTGGLEAVEGTVHRPGGGEETLDDDVVVTRTFEGEYEGRPMPEQALIVGDVALIGCCHFDLEELLGEYEPKTVIGGLHLMGAPEEELERTAELFREYGVREVYACHCTGLSESAYLAKVVGGEPAYVPMRMRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-28" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB9</accession>
    <name>RS11_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps11</name>
        <name type="ordered locus">MK1473</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB9"/>
    <dbReference type="SMR" id="Q8TVB9"/>
    <dbReference type="STRING" id="190192.MK1473"/>
    <dbReference type="EnsemblBacteria" id="AAM02686">
        <property type="protein sequence ID" value="AAM02686"/>
        <property type="gene ID" value="MK1473"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1473"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1629"/>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_S11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000123272" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="137" mass="14696" checksum="EFC8C4E910B8B038" modified="2002-06-01" version="1">MAEKEGKKEKKERWGIAHIYASFNNTIITITDLTGAETFARWSGGMVVDADREESSPYAAMKAARRAAEEAMEKGITAVHVKVRAPGGHGPKTPGPGAQAAIRALARAGLKIGRIEDVTPIPHDGTRRPGGKRGRRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD2</accession>
    <name>SYS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type-2 serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">MK1460</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 Zn(2+) ion per subunit. This ion is coordinated with 2 cysteines, 1 glutamate and a water molecule that dissociates from the zinc ion to allow the coordination of the amino group of the serine substrate, which is essential for catalysis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is presumably involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-2 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3W3S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="A=1-527"/>
    </dbReference>
    <dbReference type="PDBsum" id="3W3S"/>
    <dbReference type="AlphaFoldDB" id="Q8TVD2"/>
    <dbReference type="SMR" id="Q8TVD2"/>
    <dbReference type="STRING" id="190192.MK1460"/>
    <dbReference type="PRIDE" id="Q8TVD2"/>
    <dbReference type="EnsemblBacteria" id="AAM02673">
        <property type="protein sequence ID" value="AAM02673"/>
        <property type="gene ID" value="MK1460"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1460"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1616"/>
    <dbReference type="HOGENOM" id="CLU_542524_0_0_2"/>
    <dbReference type="OMA" id="YYVCPPK"/>
    <dbReference type="BRENDA" id="6.1.1.11">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01278">
        <property type="entry name" value="Ser_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004503">
        <property type="entry name" value="Ser-tRNA-ligase_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041293">
        <property type="entry name" value="SerS_tRNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18490">
        <property type="entry name" value="tRNA_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00415">
        <property type="entry name" value="serS_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000286169" description="Type-2 serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="349"/>
            <end position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="360"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="366"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>L-serine</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="1">
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            <position position="478"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="485"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        </location>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="444"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="3W3S"/>
        </source>
    </evidence>
    <sequence length="527" mass="61368" checksum="7526147D81EFC5CD" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD5</accession>
    <name>Q8TVD5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1457</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MK1457"/>
    </dbReference>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="286" mass="32099" checksum="AA7ACE41BD643403" modified="2002-06-01" version="1">MTDLNLSLFRRDCRFVLEELGCVVEEVDSDYQLDFVVRSDDFCFALVCVGGLSDLASISALGEWEEGLAEIARDAEALGLGALVVFPREPREEVVSVSAFCEHYGLGLAVTEWETLPLEVIEDPFRLDYGSATEVFLEAKLGRIPEWPDTEPLEDLLVGDRPVTPGDVVQVVKREVSLDWRDLVMRLRWAGYSGEGLSEALYSALMSDEVEMTEDGELMHVPERLSEVLGALLVWLQNRGRVPEDEVYEFLFAQFGVPYDVTYVALKKLEERGEIEVKRGLVVAKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE6</accession>
    <name>RS6E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps6e</name>
        <name type="ordered locus">MK1446</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE6"/>
    <dbReference type="SMR" id="Q8TVE6"/>
    <dbReference type="STRING" id="190192.MK1446"/>
    <dbReference type="EnsemblBacteria" id="AAM02659">
        <property type="protein sequence ID" value="AAM02659"/>
        <property type="gene ID" value="MK1446"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1446"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1602"/>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="OMA" id="DGFPMRP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_S6e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_S6e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_S6e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="PTHR11502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137349" description="30S ribosomal protein S6e">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="130" mass="14322" checksum="166ECAC9D304F624" modified="2002-06-01" version="1">MPEFKVVVADPEKARSYQVEVKGEDAEKLIGKRIGDVIDGEIVGLPGYKLKITGGTDKDGFPMRPDIHGPVRVRLLLSGPPGFRPERKGERRRKTVRGNTISEDIVQVNTVIVEYGDKPVEELLGEGGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH5</accession>
    <name>COBQ_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobQ</name>
        <name type="ordered locus">MK1414</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVH5"/>
    <dbReference type="SMR" id="Q8TVH5"/>
    <dbReference type="STRING" id="190192.MK1414"/>
    <dbReference type="EnsemblBacteria" id="AAM02627">
        <property type="protein sequence ID" value="AAM02627"/>
        <property type="gene ID" value="MK1414"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1414"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1570"/>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="OMA" id="EIHHGVA"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141349" description="Probable cobyric acid synthase">
        <location>
            <begin position="1"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="GATase cobBQ-type" evidence="1">
        <location>
            <begin position="249"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="331"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <sequence length="494" mass="54561" checksum="90CE8D5F1C8D84E0" modified="2002-06-01" version="1">MSAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAFQAAMAGQEPSIHHNPVLLKPKGELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAEILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADVILVADISRGGAFAAVYGTIELLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEALDTHSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRTTISDLEELRKRGMDEEVVQAADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGLLDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEGRTYTDEPHLVRLRSGYGNRGCFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVALEIVESTDLPELLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ9</accession>
    <name>Q8TVJ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted Rossmann fold nucleotide-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02603">
        <property type="protein sequence ID" value="AAM02603"/>
        <property type="gene ID" value="MK1390"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1390"/>
    <dbReference type="HOGENOM" id="CLU_107614_1_1_2"/>
    <dbReference type="OMA" id="ANPYVDI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005268">
        <property type="entry name" value="CHP00725"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041164">
        <property type="entry name" value="LDcluster4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18306">
        <property type="entry name" value="LDcluster4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00725">
        <property type="entry name" value="TIGR00725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="16233" checksum="72D160F168E8F8C5" modified="2002-06-01" version="1">MQISVIGSGRASEDVLNLAEELGREIARRGHVLVCGGRGGVMEAACRGAREEGGITVGILPGERRDEANPYVDVVIPTGLGEARNALVVRAGDAVIAVAGGWGTLSEISLAKKMGKPVVGLTSSGGWAEELARRGEIEGAESPEEAVEKAELLAGYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL0</accession>
    <name>Y1379_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0219 protein MK1379</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1379</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. UPF0219 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVL0"/>
    <dbReference type="SMR" id="Q8TVL0"/>
    <dbReference type="STRING" id="190192.MK1379"/>
    <dbReference type="EnsemblBacteria" id="AAM02592">
        <property type="protein sequence ID" value="AAM02592"/>
        <property type="gene ID" value="MK1379"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1379"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1534"/>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="OMA" id="YAKHKGK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="UPF0219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="FabH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057615" description="UPF0219 protein MK1379">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <sequence length="350" mass="37598" checksum="FF7E0EA466074645" modified="2002-06-01" version="1">MIPSERVGIVGYGAYVPRYRIKAEEIAAVWGDDVDSIKSGLMIEEKSVPSETEDSATIAVEAAKNAVARAEIDPKDIGAIYVGSESPPYAVKPTATIVAAAIGATPDLTAADYEFACKAGTAAIQTCAGLVASGMIKYGLAIGADTAQGAPGDPLEYTAAAGGAAFVIGRKKLVAEMEGTYSYTTDTPDFWRREGQPYPRHGGRFTGAPAYFKHIIRAARGLMEELDLSPEDFDYAVFHQPNGKFPRKVARSLGFEPEQVEPTIVVDRVGNTYSGSSLLGFTAALDRAEPGDRILVVSYGSGAGSDAFSFVVTERIEEVREKAPLLEEYLEDRVYVTYGEYAKMKKKLKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM0</accession>
    <name>SELD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Selenide, water dikinase</fullName>
            <ecNumber evidence="2">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Selenium donor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Selenophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">selD</name>
        <name type="ordered locus">MK1369</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + hydrogenselenide = AMP + 2 H(+) + phosphate + selenophosphate</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1369"/>
    <dbReference type="KEGG" id="mka:MK1369"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1522"/>
    <dbReference type="HOGENOM" id="CLU_032859_1_0_2"/>
    <dbReference type="OMA" id="DNEPKYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023061">
        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="PTHR10256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0711">Selenium</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000127646" description="Selenide, water dikinase">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="1">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00625"/>
        </source>
    </evidence>
    <sequence length="334" mass="35918" checksum="1472409D983757D8" modified="2008-02-26" version="3">MSRKKSLVEMADLHGUACKLPQGDLEDLLKGVELPEEGGRVEVGVGDDAAVIRVDGGYVIQSVDFFTPIHPDPYTQGRIAANNSINDVFAMGATEVLSVLVVSGFPRELPEEDAREMLQGFADQCREVDALIVGGHTIMNPWPILGGCVTGFAERYVTVGGAEPGDVLYLTKPLGTQPAMAALRLPEDVRKQFLTDSELEEAVDLAVEVMTEPLKDAAEAALEVGVHAMTDVTGFGLKGHAGEMAEASGVRVVIERLPVIPGTTELSRALGYGLERGESAETAGGLLVAVPEEHAEDLEDAFERRDVWYRRIGRVEEGSGVEVRGDVEEVEDYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM8</accession>
    <name>Q8TVM8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-10 family, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02573">
        <property type="protein sequence ID" value="AAM02573"/>
        <property type="gene ID" value="MK1360"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1360"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1508"/>
    <dbReference type="HOGENOM" id="CLU_1801704_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="242" mass="27077" checksum="179B6EA82AF53052" modified="2002-06-01" version="1">MRLWESLVLAMLSVPEDPGEWLGPVLGHPEVLLAKVGRGGGRVGIKFPRIGRTSKSERLPRVKIKNWGYYPEREREERERRERELLSLVYWKVWLYHRMVPPYLMKAFVVLLLGGLGLVALYCLGVLKRTWRYALLTSVLAAVLGVLPEAVMTNYAVEDFSNAMLAGGGLQDCLHAFKVWGVYGLWTLLAAYVMLVLAAVVLARNALRDVPVPVLALSLAILAVPLVTGIWALDWGLWVTMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN2</accession>
    <name>Q8TVN2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-10 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1356</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02569">
        <property type="protein sequence ID" value="AAM02569"/>
        <property type="gene ID" value="MK1356"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1356"/>
    <dbReference type="HOGENOM" id="CLU_910936_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="309" mass="35210" checksum="C901AC3FD80514A3" modified="2002-06-01" version="1">MEPLTLVLLVFPGDPGEHLGHALLEHPEILSAVGGGRGGVAGGRGGGGRGGAGVKLSRAGKVLKSPKKFYRVLKRLKRRKHRPERERRRLLLLYWKVRIYDRIVPTALILSGFISFFVVPPVLDFLSDRLLRKAWERKYGRRFRRTREWRHRCDYELSRLCDDVTYRLFLTSLLGFLVVGVEGIFSTWAVEAFADAVLRGEGFRAALDAFRWYGVLGMEYLLAAYAALCVAIAASAVARTWERRRTERLREEVRRRLGRRPEKSDPPSCIPESFRTSVSRVTRALEALLLILPLVTGTWALRWGLWVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN6</accession>
    <name>Q8TVN6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-35 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02565">
        <property type="protein sequence ID" value="AAM02565"/>
        <property type="gene ID" value="MK1352"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1352"/>
    <dbReference type="HOGENOM" id="CLU_1340741_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="21995" checksum="14EA0C9438B11098" modified="2002-06-01" version="1">MTASTLITESTFDALSGLEPPPEVGILVITAVIATVPTVVAIRIGGILGRVVAYFLTVALTFSALPLILHIQRGLELGPRELLTLALMMSTCSLSILRLATEGLKLTLGSAVAVVNLAGGFALLVVAVTGGWESLELALSSHHWFRLMVVPAILTVLTYWVYVAVRDVLDWLRGRRADPRDLEAAEALLEGVRACERRLRRVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU4</accession>
    <name>Q8TVU4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1294</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02507">
        <property type="protein sequence ID" value="AAM02507"/>
        <property type="gene ID" value="MK1294"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1294"/>
    <dbReference type="HOGENOM" id="CLU_638753_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="46958" checksum="1AF067ED3B7D1AD9" modified="2002-06-01" version="1">MRTLPIALTLLLVPAAAHALQPEVLWKGEFPDCPGSGFVHLVPVENEENTALLVVRTFERWSLYEVKGSEVTHLGDVDLATPRSTEVTGAMYYPDRNELLVTAVIEEEGEGPGGGVLAIRVGRDGVDARKFDGAYVHARPCPDGTILALLHEGHEYLEDEGVHRHDLALIRLYPDGRAERLVTFDVFSTLPPSALLIPGTLRIEPLGDDYLITGSDLVSMVELRRTASGWDTRYYGPMNLGARITYYLEESTVLDPRKRLVLVRQSNLDEPLEGYWAICELVPESGLGGPVLKELRRVDLGGDDLYFPQSGVKLEVGGSTYYVVPAVGASSTKLLVLGPDTAHVWEVPGFRELAVTPRLYGARVSERETGGCTVEVCAFKPSRGEEWLTPLFGGTGEGEKKEEHKENEGGKVAIVPVLPAIPFRRPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU9</accession>
    <name>Q8TVU9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted adenine-specific DNA methylase containing a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02502">
        <property type="protein sequence ID" value="AAM02502"/>
        <property type="gene ID" value="MK1289"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1289"/>
    <dbReference type="HOGENOM" id="CLU_369911_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="752" mass="83961" checksum="7B5A984D7F571A97" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW0</accession>
    <name>Q8TVW0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-19 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02491">
        <property type="protein sequence ID" value="AAM02491"/>
        <property type="gene ID" value="MK1278"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1278"/>
    <dbReference type="HOGENOM" id="CLU_482855_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="564" mass="63876" checksum="C40E64943A1F8B29" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW5</accession>
    <name>Q8TVW5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02486">
        <property type="protein sequence ID" value="AAM02486"/>
        <property type="gene ID" value="MK1273"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1273"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1389"/>
    <dbReference type="HOGENOM" id="CLU_701341_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="393" mass="42537" checksum="7860F5EB458DEE0B" modified="2002-06-01" version="1">MVPAARRAIPTLVSLASLVVVLTVPVSGEEYALRDHGSGLTTVWFYDPNTMGSQLPSNWKDYLWVGKPTSVALGFELTANNCTSYFDSPFAHDIVGRLTAQGFDVTSVDYAEANGPVSSPDDLLNATNRRGTPLTPAGLSLRVTDGTSTYYLIPCYGGPDRVPYYLDEDSWPAKGERTVIWVPTTSGPVPFLLRGADEPNALLVVERPGSERLDLDGDGNPDVDLSGLASLSMVWMLALTIRTVTGDYLLVQGPDLYYEVSPDEHLLNVASFRASDVEFSDDGTMKLKLHIGVEKGTSVSLEGVDLAFLDRDGTALNVTPSEGDLENELLNSLKSALSWDSSEITLSHPSGDIREHLPAIITLRVMGSTELTDNLGNTSQFTVMASGRWTSYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX8</accession>
    <name>Q8TVX8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase of the AAA+ class</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02473">
        <property type="protein sequence ID" value="AAM02473"/>
        <property type="gene ID" value="MK1260"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1260"/>
    <dbReference type="HOGENOM" id="CLU_382951_0_0_2"/>
    <dbReference type="OMA" id="ELAHYDD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007555">
        <property type="entry name" value="DUF499"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04465">
        <property type="entry name" value="DUF499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="702"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="722" mass="81933" checksum="7FA609E9868A997E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ6</accession>
    <name>Q8TVZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02455">
        <property type="protein sequence ID" value="AAM02455"/>
        <property type="gene ID" value="MK1242"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1242"/>
    <dbReference type="HOGENOM" id="CLU_2820824_0_0_2"/>
    <dbReference type="OMA" id="VIEVPYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="8134" checksum="DD3BD95FA1CED490" modified="2002-06-01" version="1">MIRRDVKVFRCSRCGEIIEVPYGAPKPSSCPNCGAPAVFIHRLNPGPGTWGSYAGLGRGMGRGGPGRGGRGGPGRGGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW03</accession>
    <name>Q8TW03_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted endonuclease of the RecB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1235</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02448">
        <property type="protein sequence ID" value="AAM02448"/>
        <property type="gene ID" value="MK1235"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1235"/>
    <dbReference type="HOGENOM" id="CLU_1076104_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="258" mass="28431" checksum="1D8CF00760FA3BAF" modified="2002-06-01" version="1">MLRRGKSAEEIAASILRKEGFEVVARNYRVELEDELVAEIDIVAEKDGERYAVEVKAGTVGVDAVRQAYVAAKLTGYRPLVMGRRVHPSAEALADHLGVEVREFSEFVEVEPVDELAAVVSDLLQDKMLVLLSAASNVSWEDLYAALQGDLRRVTELVRVRDRSQAEELIEALAFLKLMASETVGAVKGVEGDEFLLELDPRVEVPDGTTLVVLNRWGSPAFVLAEPVSVGTHRARMRSWEGKVETGERVVYLGEVEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW15</accession>
    <name>RS8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8</name>
        <name type="ordered locus">MK1223</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW15"/>
    <dbReference type="SMR" id="Q8TW15"/>
    <dbReference type="STRING" id="190192.MK1223"/>
    <dbReference type="EnsemblBacteria" id="AAM02436">
        <property type="protein sequence ID" value="AAM02436"/>
        <property type="gene ID" value="MK1223"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1223"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1326"/>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_S8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000126541" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="130" mass="14787" checksum="0C23D37053EB1BEC" modified="2002-06-01" version="1">MTLMDPLADAMATIKNNEMVGNKECVIEPASKLIGRVLKVMQEYGYIGSFEFIDDGRSGKFLVKLVGRINDCGVIKPRHPVKKDEWEYWEQRYLPARDFGLLIVTTPEGVMSHYEAKERGIGGRLLAYVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW25</accession>
    <name>UPPP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="synonym">bacA</name>
        <name type="synonym">upk</name>
        <name type="ordered locus">MK1212</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW25"/>
    <dbReference type="SMR" id="Q8TW25"/>
    <dbReference type="STRING" id="190192.MK1212"/>
    <dbReference type="EnsemblBacteria" id="AAM02425">
        <property type="protein sequence ID" value="AAM02425"/>
        <property type="gene ID" value="MK1212"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1212"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1314"/>
    <dbReference type="HOGENOM" id="CLU_060296_1_2_2"/>
    <dbReference type="OMA" id="IEGPFRD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000151250" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="269" mass="28622" checksum="F87F59EACB982AF3" modified="2002-06-01" version="1">MREIELWTAVLAGVVQGITEWLPISSEGQATMTMMKVLGIPPSTAMDLALWLHAGTLLAVLLRFGVPYWLTVRDLLMGGPWRRLGLFAIVATVCTAVVGLPVYKVLKGIFSAATGDAVQMAIGGALIVTGLLLRISPEGLRDRREVNVVDAVIVGLGQGFSVIPGISRSGTTMALLLWRRFDGGEAVWLSFYLAGPAMLGATALELKEGLSAATKMGTSWMVTAIGVSFVVSLICMEVLLRVARRLDFSKVCLLLGGIALLVPLAAKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW33</accession>
    <name>Q8TW33_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02417">
        <property type="protein sequence ID" value="AAM02417"/>
        <property type="gene ID" value="MK1204"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1204"/>
    <dbReference type="HOGENOM" id="CLU_2490476_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="86" mass="9028" checksum="BFFBBCCBA529DD1E" modified="2002-06-01" version="1">MCSVSLDGLLETPIDRRTFLKLAFTGVLSVLVSEEPVRALIRSVPRSVVLVFGGSCGGCTTTLAEIGIDLKASPTWIPRSGLSGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW53</accession>
    <name>Q8TW53_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02396">
        <property type="protein sequence ID" value="AAM02396"/>
        <property type="gene ID" value="MK1183"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1183"/>
    <dbReference type="HOGENOM" id="CLU_1060115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="168"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="262" mass="27653" checksum="8F2A7F1EEB734D5D" modified="2002-06-01" version="1">MEIRKLRVAVQLISAGVAAVGVAFGLKVVVPGIAAGCAGIIDTGTMLLALPVVAAGLVTLTQLAAVALVGWVVPAILVGGAVCGWICPLVLVQDALAGLGRKLGLTVHRPPGHGVLLYLRYVILAATLVLGPFAAYKLWYKICPMHYLSYILLGRQEIGLYSVLKMSAAFGILGASVFVPRFVCRYICPVGVLLRLANGWSLLDPLLKGKTEIVRKGCKGCKVCTVCPKALDPADAFPNDFDCMRCGRCLDCPIHLKMVGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW56</accession>
    <name>Q8TW56_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02393">
        <property type="protein sequence ID" value="AAM02393"/>
        <property type="gene ID" value="MK1180"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1180"/>
    <dbReference type="HOGENOM" id="CLU_876065_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="317" mass="36240" checksum="AC699F0A12ECAEFB" modified="2002-06-01" version="1">MNQGPTVLRCWQRPPTCVPEPHRLTLRNIATIHDPRGLISGTISRVLSGAESGAQGWIYGKKAIGWRERSAELELATLNNPTFGVGRLKAEEPDVEWEHWIVFHSPVGKVRIGDVELPTEEPLTVKSIEVDCDSKLMKINFKDDARSLMRLEGDDDHSWELKKCSANVKFNMSPKVEDEEVKCIYEIRAEKENFSLKTIDNVIEYLQEKEVFSCEKIWEKPEKAKSKSRVSKYIEILVEVELSNFIDKVSKVDVDYRLNGKKKFKVVDAELLVVLNKDKVKKELLERLYGVSGDIDFADEVLFLTFAPHARFKSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW59</accession>
    <name>Q8TW59_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein containing a metal-binding domain shared with formylmethanofuran dehydrogenase subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1177"/>
    <dbReference type="EnsemblBacteria" id="AAM02390">
        <property type="protein sequence ID" value="AAM02390"/>
        <property type="gene ID" value="MK1177"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1177"/>
    <dbReference type="HOGENOM" id="CLU_398832_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003814">
        <property type="entry name" value="FmdEsu_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02663">
        <property type="entry name" value="FmdE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FmdE" evidence="1">
        <location>
            <begin position="249"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="FmdE" evidence="1">
        <location>
            <begin position="604"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="810" mass="89562" checksum="5F01FBE30E03CE88" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW63</accession>
    <name>Q8TW63_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-27 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02386">
        <property type="protein sequence ID" value="AAM02386"/>
        <property type="gene ID" value="MK1173"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1173"/>
    <dbReference type="HOGENOM" id="CLU_1154356_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="240" mass="25400" checksum="59813D9792655BEC" modified="2002-06-01" version="1">MRSFPLLPVALVLLPAAAHAGKISVTVYPESVNVQGNALGIHERLEKELDLIVTVTDLGTGAVYDMKFNPLEPSSPAGLDVTGPVTVIATVRYARNIVTRFNVWPEGASSFSSVDVPEGRLETTVNHPRISVPSTGTFDVECPITMSLRTDKGTVTVKLLLHVSGIDVKGVKAERKGVLPELPSVVRLIPVAVALAPMVVIPGFILIDLTLNVLNGLKSTLGPVLDHLFGVLGGLLPRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW72</accession>
    <name>Q8TW72_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted nuclease of the micrococcal nuclease (Thermonuclease) family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02377">
        <property type="protein sequence ID" value="AAM02377"/>
        <property type="gene ID" value="MK1164"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1164"/>
    <dbReference type="HOGENOM" id="CLU_1507391_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="178" mass="20064" checksum="C63D722A94B1F5A7" modified="2002-06-01" version="1">MPVAYFDGKPAVKIRFARVNAPEIYHPSSEEEYRLGLKAKEFVEKKIRDAGGYVKCRAYGLGKYHRIIADVYPDDEETTLSELLVERKLAEYRQYPEPDPLPRWFPREFKAHVAKVVDGDTIYVYARNGEEGPVPFEEKESMRGTVVGEYKTGTPTVPVALAVISAVIGVGLLVSARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW73</accession>
    <name>Q8TW73_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1163</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02376">
        <property type="protein sequence ID" value="AAM02376"/>
        <property type="gene ID" value="MK1163"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1163"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="24096" checksum="61F4F78DAEC3E738" modified="2002-06-01" version="1">MVESHAHVPIPSDPVERIRALRVLREVYSRGKRPSLEVTYRTVNGSTCGPYYVARWRRDSRHKCGRTLYLGKPENESVRFVEWLVSLNRAEVLELARHLMRNLRSVLKTLLTEVSSLPYKQARRVLARGLALTFNARPSNSPQIRDLLEELPDRLESFAVRTLGGWPAHYSAHLNKIIRFRKRSLDGKHEVPDVMLELERWKLRHGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW78</accession>
    <name>Q8TW78_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-9 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02371">
        <property type="protein sequence ID" value="AAM02371"/>
        <property type="gene ID" value="MK1158"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1158"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1239"/>
    <dbReference type="HOGENOM" id="CLU_679008_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="405" mass="44306" checksum="17DDD23FF3A63269" modified="2002-06-01" version="1">MGDPGASGCELPLVSRETVSRLRLAYGALCTLSAPLGLLGSDAGVMVAGLGSIVGVLLGLMRPFSFRERAPKPEHARIGTRLLYPTASFNRLRDGPKLGTALPLAMIPALILFFTMWLSTGDRTGVALTVFLLISIAATTVFSYLVKLSAAMTLGYALPVRVKPEGTRVSLASLGYCEAELVTDGNVKVSGFRELGSYPVEYGGLPGDPPGDLRIRVGYFPPGKHEVEVRDCDFLFLRTRGVCMLYDSRGLPEDREGAFHLARWWEKFSMAYAALCLREGVPGAVELERWLERVRNNRAVGFLVRYMNCDFNLPVVEMCVLVLSLLPMTEAVDFVLTRGVRGWGALDAMVVLDAFTLCLLALPVLCGLLLNRRAVRSGRVYECLLEGEPPHRRLLSTLCVYNPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW82</accession>
    <name>Q8TW82_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-9 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02367">
        <property type="protein sequence ID" value="AAM02367"/>
        <property type="gene ID" value="MK1154"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1154"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1232"/>
    <dbReference type="HOGENOM" id="CLU_679008_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="419" mass="47003" checksum="34B3A179D6BC1BFA" modified="2002-06-01" version="1">MRGLTRFAERRLRWYRRAYWTLCPAVVLFTGLGWEEAGLLLMGFAAFAGAFYGIYREETEGTFPRSEHSRRVGRTLMWYPTASFDRVLGGLTPTTFLPLFPALLYFITSVARHPDFLHSRIAVALFFLGPLPVLLYLVRYEVASALGCVRVLRVRGSRALLLSLGYCEAELVTDGNVKVSGFRELGSYPVEYGGLPGDPPGDLRIRVGYFPPGKHEVEVRDCDFLFLRTRGVCMLYDSRGLPEDREGAFHLARWWEKFSMAYAALCLKEGGPPDPGEAEALAAGAGDNARRALTVTYFGVKFPVGKLVASEEAMLGVVLGMIPIATLVRDLLPPDLSLLSAPIAAPLAGVLLALPIAWRNRRVVRTGRWYRLLLRGDHVEKLLPRLLGEDLWSRRGGMITTLWKVFDVLERERRQHPPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB1</accession>
    <name>Q8TWB1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02337">
        <property type="protein sequence ID" value="AAM02337"/>
        <property type="gene ID" value="MK1124"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1124"/>
    <dbReference type="HOGENOM" id="CLU_843620_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="329" mass="34867" checksum="CDA2560FB32DDE7B" modified="2002-06-01" version="1">MRMIAIYDLGWFSRALLADATVAVIGLTIVATLIAYLGLGGPLRPSLAVGAAAGALGLVIYWYGTLLASSCRIRGVGRTDWGVVVGVKCRTSSVAVVGILGEGEIGVERGRIVKRETQYGLCLYKLVLPRGEGLLWVYGQPEGLVVATVGNGVAVFSDDLPRVLYLGVIATGSLLEWFLDVVMPFSTLADLAMEPAWRIVRESSSISCAPSWDRLAREVAVDPGMTARALEGRVALVHRDVALFFLSVLIVVLGVPVALSVLSGYGPVLYELIEPLVAVLQGVCGGTFPVLICTLLAALLVGYAVLLLTSPLLLTYVGFVTRLTADRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB7</accession>
    <name>Q8TWB7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02331">
        <property type="protein sequence ID" value="AAM02331"/>
        <property type="gene ID" value="MK1118"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1118"/>
    <dbReference type="HOGENOM" id="CLU_798355_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="347" mass="37337" checksum="572BDA4695455C26" modified="2002-06-01" version="1">MCSCTHRAVLIEYREHVVVSVTGGRIAVSLPDGSTVEVPADSCLLELGSPDMAAYRVGNRHYVGFPIAVTYRGRIYLLGSFRLTMGDAPIVERYAMRVLRNGVEFDVGYSEITVYEPRTLPVDRVLAPFIRAAYSVRCTSAELRDGVLGLTLEVTEARTVEVSQGKSRAPVERYLLLCDLRAGTCRLAGPVGGSPTDDDHGIVCAYDLNSGELTFTIVGDSLPRLPPLLEELGADPAVITSLKLVGKVLSGDYWATSWDYWNLVKRASDPAVRHILERSGIVAAVPMRVVLHPELLLGGYLLAAGTIAGTSLGLVWLVGPDICRDAGPPRHFPGACFESSTGCSCTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC6</accession>
    <name>Q8TWC6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02322">
        <property type="protein sequence ID" value="AAM02322"/>
        <property type="gene ID" value="MK1109"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1109"/>
    <dbReference type="HOGENOM" id="CLU_985577_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="282" mass="31648" checksum="F71514F07F8F8009" modified="2002-06-01" version="1">MSGTETVRLRAVSPVTSRTGESVVVDLREDLRRHLRALLGRCLERGEVRRIETALFGGTVNGETIFPAVEFRLVGDELPRYPAGSVFELEVRVESRELPWSILSRYSDDPVEFASECLGWALELLSLLGGVGAQSDLCAGSLLWEGREPRRPVDVLSRTDAALRWVEAKFGDVPDRSPRFAAFRDGYFLVAVGPTGDLYEVLGRVESVYNRLARARFRLVLQVKRTRDGLHFPVAVAFARHARERVELQRAAATFLDLGWETLPKLLDLAAESGRRSRPIRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF2</accession>
    <name>Q8TWF2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1083"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02296">
        <property type="protein sequence ID" value="AAM02296"/>
        <property type="gene ID" value="MK1083"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1083"/>
    <dbReference type="HOGENOM" id="CLU_009583_32_0_2"/>
    <dbReference type="OMA" id="VSSWVNQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022622">
        <property type="entry name" value="DUF3492"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11997">
        <property type="entry name" value="DUF3492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF3492" evidence="2">
        <location>
            <begin position="14"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="312"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="491" mass="56016" checksum="C60D66BDEAD14BF9" modified="2002-06-01" version="1">MVEFRGSGMYKRPTVTVVTEATYPVALGGVTTWVQRLIKYSPDVVFNVLCMTGPERTEPVVEIPANVRDVVIQEIVPKGNKLRRWLDRITPTHRLRWSTAAKTLRATFERMVEGEPLSEPMLKELWKVSKSPTSVLASSTMYELARYVHYLASQYEDKCENNPFSDVFWVAVNIASFVLGAAAGARRLPNCDVAHAQNSGVCGFLCSVAKAVRNVPFIITEHGILLRELDTRLEGYGRTTRELFKECFRSMMLTSYEQCEEIIEISDYHAELALEQGAPEDKIKVVYSGIETWKYSPGDLERKYNEPEVLEVGTITRIERVKGIDVLIEIAARTVEHIGNVVFHVVGPVEDEAYYEECRKLVKEYGLEDIVRFHGPCTPDEVVKWLRRFHIFLLPSRSEGLPMALLEAMSCGCPVVASEVGAVPYIVDRNFGRTFRSEDADEAARHLVRLLYDPELMFEMAHHATKRAKQYDVMRMCHDYFREYCKYARGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG2</accession>
    <name>CDPAS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDP-archaeol synthase</fullName>
            <ecNumber evidence="1">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carS</name>
        <name type="ordered locus">MK1073</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG2"/>
    <dbReference type="SMR" id="Q8TWG2"/>
    <dbReference type="STRING" id="190192.MK1073"/>
    <dbReference type="EnsemblBacteria" id="AAM02286">
        <property type="protein sequence ID" value="AAM02286"/>
        <property type="gene ID" value="MK1073"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1073"/>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="OMA" id="GKTWRGT"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="PTHR39650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000094171" description="CDP-archaeol synthase">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <sequence length="173" mass="18347" checksum="F02F81BED885CD48" modified="2002-06-01" version="1">MVEMGSGAWLELGKGLWFILPAYIANLSACLFGGGRPLDFGKKLSDGRRLLGDGVTIRGFIVGVLAGAVVGLGEGLVVGDPWKAGDGFILGLGAMAGDAVGSFVKRRIGLERGAPAPVLDQLDFFVGAVLLYYLVYGWHPPGWVLVGLAILTLALHWLTNVIGYLLKLKEVPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH1</accession>
    <name>Q8TWH1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gltB_2</name>
        <name evidence="2" type="ordered locus">MK1063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1063"/>
    <dbReference type="EnsemblBacteria" id="AAM02276">
        <property type="protein sequence ID" value="AAM02276"/>
        <property type="gene ID" value="MK1063"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1063"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1117"/>
    <dbReference type="HOGENOM" id="CLU_077077_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="313" mass="34315" checksum="6301574DD26E137A" modified="2002-06-01" version="1">MACGIAGIVLPEPGPVGAILTEMLDALQHRGPDSAGYGLYRDVDTAVFVLELPAEDGEMELLSEVERALEGRQLKRVERIAEDAGTRVYRLFVEGFSTGRQGQRELAEVVEKIENSDITVLSAGHGFEILKDVGTAAEVSEQYGVEAIEGTHGIGHVRFSTESEVDRYHAHPFQSYMIPNMAVVHNGQITNYYTIRERLEIKGYQFKTNNDSECIVVYVADKLRDGYSLEEAMEEAIRDLDGPFCFIISTPDAIAVARDPLGLRPGVIGFGKDGAVAVASEEVALRRIFGDELEGIEQIEPGEYEVFEVGGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH3</accession>
    <name>Q8TWH3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyltransferase (The isoleucine patch superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1061"/>
    <dbReference type="EnsemblBacteria" id="AAM02274">
        <property type="protein sequence ID" value="AAM02274"/>
        <property type="gene ID" value="MK1061"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1061"/>
    <dbReference type="HOGENOM" id="CLU_884580_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="314" mass="34555" checksum="54CF77A391E84AAE" modified="2002-06-01" version="1">MRRREVLRPLREDDKYRHPAVLDEGVKIIGDNLADVTAEIGAYAEIGPSVVIRRKAAIYGFCRVFDSDVGERASISPFSIVRADVGNDAFIGDGSMIGAIGEDRAKLGYDCFIGMRCVVYGGVKVGDGAIVGAGSVVEEDVEPYTVVMGRPAEYVGDTVRISANTFVGGEEASAQARMVTRGYDTGWETLGCTESRGVNLTVVTFDREVWERLMGLLGRFRGEVDHGTFRFEGVEFFGIAASSERPPRRVVDKIRELLLERAERLVACILDCPKGYGRDVVVRSGRISDERVTGPLRARGSRYYRRTEEGLHTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ9</accession>
    <name>Q8TWJ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02247">
        <property type="protein sequence ID" value="AAM02247"/>
        <property type="gene ID" value="MK1034"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1034"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1086"/>
    <dbReference type="HOGENOM" id="CLU_563395_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="484" mass="55517" checksum="A66F692458DE2005" modified="2002-06-01" version="1">MEAVVLFPAGPRDVIVGGGNLFEASRRLYERALSDPSALGEASFPTLDAIIRFVLYEEGYDPREITLISLGTRRLDHQYGHHPEDTEYCARLVSLYAEKEWGVRVEDPVVLRKDPDRFEVFREIEDVLKTLHRREDLKRSELYAYVGGAHPNVGWAIRLIGATLWEDRFHPLRVLDENVEVEPSPLILPMKTMKCVLIEHGLFEVAAGIAERFSAYEDRMEPVYLRARDSELNFNYEEARRLYAEVTRSAHLDELRHEARRRLRKLREAEKDPIRRIIHHMRVVLENARYQWIRGEFGDFIVRLSMLYENYTQACFMLLYERLSGERVTTSDPVELASLLADLLGSSEELSGAVEDRLDSDIEAVLRELRGEEGEGRAVLAFLHVAQKVLSGALEVGAVEKDVFGPLSRYIQVLSCSELRKARHLFAHEGRGVSREDVERIVKAMARPCRVNFETIDELLSFLEEGIDRLELAVSEVRGTDRGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN4</accession>
    <name>Q8TWN4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted cysteine protease of the transglutaminase-like superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02211">
        <property type="protein sequence ID" value="AAM02211"/>
        <property type="gene ID" value="MK0998"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0998"/>
    <dbReference type="HOGENOM" id="CLU_1369538_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="76"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="199" mass="22067" checksum="2A576D26922860D2" modified="2002-06-01" version="1">MVAYQQGGLVAFKLFVYPPLTAVPPVLPQIKPGEDELSVVRTLALFVSDTMQYDVGAEPPEYVPPVLRRAFAELELRGRWRGACGTASYMFSYLCRLYGIPTRLVLIEPTDTGTVVSGTGRERERFVLHAQVQVWVHGRWMTVDPSVGLVGTSDRPVQGHRVRPDGTELYTPGVKSYTVCTQGPLVFWDTLTGLVELIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN7</accession>
    <name>Q8TWN7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylmuramoylalanine-D-glutamate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">murD_1</name>
        <name evidence="2" type="ordered locus">MK0995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0995"/>
    <dbReference type="EnsemblBacteria" id="AAM02208">
        <property type="protein sequence ID" value="AAM02208"/>
        <property type="gene ID" value="MK0995"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0995"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1043"/>
    <dbReference type="HOGENOM" id="CLU_629483_0_0_2"/>
    <dbReference type="OMA" id="VEVCDMT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="111"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="435" mass="47952" checksum="F8FD19B042A5DBCE" modified="2002-06-01" version="1">MSVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGHHDIDRIRECDTVYFSPSIPNDAEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTTVHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARPEVVVCEVCDMTLDYFSDRAPRYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRCYDELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGEGRLVIGKTDNLSALKVVLRDYGPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPGLSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGRDVGVLGNGQDVIISIQHDVERAVRFLTAGSERSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP7</accession>
    <name>Q8TWP7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02198">
        <property type="protein sequence ID" value="AAM02198"/>
        <property type="gene ID" value="MK0985"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0985"/>
    <dbReference type="HOGENOM" id="CLU_2968476_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="58" mass="6877" checksum="0FD3564391C0DF24" modified="2002-06-01" version="1">MLTRILQVIGVGPEEITVYDKKWEQYRPLVYYANSVLAREEGNLSWESDRCSRTRRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT0</accession>
    <name>Q8TWT0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyruvate-formate lyase-activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0952</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0952"/>
    <dbReference type="EnsemblBacteria" id="AAM02165">
        <property type="protein sequence ID" value="AAM02165"/>
        <property type="gene ID" value="MK0952"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0952"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.997"/>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="OMA" id="YHFYPGS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="78"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="39565" checksum="A5A52F6401AEFD44" modified="2002-06-01" version="1">MGSRTMSDGALREWEGRTWEEVDGKVRCLVCPRKCVIPEGERGFCRVRENRDGELVLLIHGKVSTAVPDPIEKKPLFHYKPGTDVFSLGTVGCNFRCRHCQNWQISQAGPEEVPLEEWPPERIVGAAKRTGCESVAFTYNEPIIGLEYTLETFEACREEGLGCVYVTNGFATRRTAKILGEVLDAANVDLKAFTEDFYRDVAKAWLKPVLRTCKIWKDMGVHVELTTLVIPGYNDSEEEARRIARWIRKELGPDTPWHVSRFHPDYRMLDVPPTPVETIEKFVEIGYEEGLYYVYAGNVPGHKYENTYCPECKEPVVVRRGFSIVKMHVTDDFHCEHCDAELHFVT</sequence>
</entry>
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    <accession>Q8TWT4</accession>
    <name>Q8TWT4_METKA</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-5 family</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">MK0948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02161">
        <property type="protein sequence ID" value="AAM02161"/>
        <property type="gene ID" value="MK0948"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_394148_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="699" mass="77316" checksum="49CAA0B8AA198328" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU3</accession>
    <name>Q8TWU3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Iron-sulfur flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">isf</name>
        <name evidence="2" type="ordered locus">MK0938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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    <dbReference type="EMBL" id="AE009439">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0938"/>
    <dbReference type="EnsemblBacteria" id="AAM02151">
        <property type="protein sequence ID" value="AAM02151"/>
        <property type="gene ID" value="MK0938"/>
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    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="OMA" id="MHIHGMI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="1">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="20660" checksum="1774F4AD01970985" modified="2002-06-01" version="1">MILGISGSPREGNTEYLVRIALEAAEEVSGEETEFITVRDLDISPCEACGECLETGECAIDDDMQDVYELMRECDGMIVGSPVYYGGVSAQLKALIDRTRPLRINWELKDKVGGAIAIGGARNGGQEHTLRDIQNFFMIHAMIVVGDSDPTAHFGGAGVGLEPGDVEEDETGIETARNTGRRVGEVVKLIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU9</accession>
    <name>Y932_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MK0932</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0932</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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            <taxon>Methanopyrus</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWU9"/>
    <dbReference type="SMR" id="Q8TWU9"/>
    <dbReference type="STRING" id="190192.MK0932"/>
    <dbReference type="EnsemblBacteria" id="AAM02145">
        <property type="protein sequence ID" value="AAM02145"/>
        <property type="gene ID" value="MK0932"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0932"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.975"/>
    <dbReference type="HOGENOM" id="CLU_508670_0_0_2"/>
    <dbReference type="OMA" id="GLRPYVY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107442" description="Uncharacterized protein MK0932">
        <location>
            <begin position="1"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="193"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="523" mass="57616" checksum="5C3A2766CAF22612" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW7</accession>
    <name>Q8TWW7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein, MK-44 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02127">
        <property type="protein sequence ID" value="AAM02127"/>
        <property type="gene ID" value="MK0914"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0914"/>
    <dbReference type="HOGENOM" id="CLU_430012_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="707"/>
            <end position="728"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="739" mass="82621" checksum="8336839028B9530F" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX8</accession>
    <name>Q8TWX8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0903"/>
    <dbReference type="EnsemblBacteria" id="AAM02116">
        <property type="protein sequence ID" value="AAM02116"/>
        <property type="gene ID" value="MK0903"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0903"/>
    <dbReference type="HOGENOM" id="CLU_152999_0_0_2"/>
    <dbReference type="OMA" id="VRDEDRC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="76"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="105"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="139" mass="15190" checksum="CE929F07722287FE" modified="2002-06-01" version="1">MTEKVRRIVRIKATGDVVRALSRVVEEGLELSILNASVSPDAVAAEIAVQAVDDEHVRNFLRTLREFGIEVEEIEKTLVRDEDRCLHCTACHSVCPTGAIELKGVEVELDDEECIVCGSCTEICPVGALRIASKEGERG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY2</accession>
    <name>SYI_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">MK0899</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWY2"/>
    <dbReference type="SMR" id="Q8TWY2"/>
    <dbReference type="STRING" id="190192.MK0899"/>
    <dbReference type="PRIDE" id="Q8TWY2"/>
    <dbReference type="EnsemblBacteria" id="AAM02112">
        <property type="protein sequence ID" value="AAM02112"/>
        <property type="gene ID" value="MK0899"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0899"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.941"/>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098582" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="628"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <sequence length="1080" mass="126028" checksum="0024CBCD26D75BC0" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ5</accession>
    <name>Q8TWZ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02099">
        <property type="protein sequence ID" value="AAM02099"/>
        <property type="gene ID" value="MK0886"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0886"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.928"/>
    <dbReference type="HOGENOM" id="CLU_1264576_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="218" mass="24568" checksum="BE0C7FE461B3961D" modified="2002-06-01" version="1">MKLPENVPEPPVVCEKFDIGEEARVMRRSHDGYVRLGEVAGLEKFRSVCLVSVRADSGYYYVVPYRVTEERVITEPLRVRKIGEGVVVPPIIPHRRLGELFVDVRKVGSDYMVTLYGKNLHVRTYESLTIMTQRRGSELSAGGMSIDLSSGFLKVEGVRCRPLGFHRPDAIVGITERLEVVEIRENGSRYLGDVVAVGEFLRITLDDLSLDVPRRVIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ9</accession>
    <name>Q8TWZ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease related to cation transporters</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0882</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02095">
        <property type="protein sequence ID" value="AAM02095"/>
        <property type="gene ID" value="MK0882"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0882"/>
    <dbReference type="HOGENOM" id="CLU_1559497_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="PTHR16228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="SSF161093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="MgtE" evidence="7">
        <location>
            <begin position="47"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="18150" checksum="957B8ECB35EBCC1A" modified="2002-06-01" version="1">MKRFLRDLSAATASLTALVLMESFTGITLESLKHRWVHHPEFLAMLPALMDFRGNVALAHSARTATATHLGDEERALQSSLAVLLIGLMVPPVIGSVVYYAYGGDLSTLVGTAFLTVLSVTAVVTPVTVGIVRMAAMLGFDPDHVAPPLTTALSDVLTVAFLFTIAEVMVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX03</accession>
    <name>PAN_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="1">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pan</name>
        <name type="ordered locus">MK0878</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX03"/>
    <dbReference type="SMR" id="Q8TX03"/>
    <dbReference type="STRING" id="190192.MK0878"/>
    <dbReference type="EnsemblBacteria" id="AAM02091">
        <property type="protein sequence ID" value="AAM02091"/>
        <property type="gene ID" value="MK0878"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0878"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.919"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_2_2"/>
    <dbReference type="OMA" id="YNMTTFE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005937">
        <property type="entry name" value="26S_Psome_P45-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01242">
        <property type="entry name" value="26Sp45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000084744" description="Proteasome-activating nucleotidase">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Docks into pockets in the proteasome alpha-ring to cause gate opening" evidence="1">
        <location>
            <begin position="434"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="223"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <sequence length="436" mass="49767" checksum="AE150086616A02ED" modified="2002-06-01" version="1">MEETGVKDVRDLCEKFLDFKREKERLEELLKEYFKRLEELERKLRAHEEKLRIEARRRKTLEKELEMERDEKAELREELRRKEVMIEKLRSDLQRMKKPPLIVGTVEEILDDGRVIVKSSTGPKFVSNVSPTVDRNELEPGANVALNQQSMAVVDVLPSEKDSRVLAMEVDESPDVSYDDIGGLDEQIREIREVVEKPLKEPELFEKVGVEPPKGVLLYGPPGTGKTLLAKAVANHADATFIRLAAPELVQKFIGEGARLVRELFELAREKAPSIIFIDEIDAIGARRMRDATSGDREVQRTLTQLLAEMDGFDPLDDIKVIAATNRKDILDPALLRPGRFDRHIKIPLPDEEGRYEIFKIHTRDMNLAEDVDLQKLAKITEGASGADIKAICTEAGMMAIREDRDIVTMDDFLKAVDRVMGKKEEESGEFKRAYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX68</accession>
    <name>Q8TX68_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02021">
        <property type="protein sequence ID" value="AAM02021"/>
        <property type="gene ID" value="MK0807"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0807"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.848"/>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="OMA" id="EIPYQKA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_Chlor/Fluor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="PTHR35092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="SSF101852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="SSF102522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="261" mass="28022" checksum="8315440645E04C45" modified="2002-06-01" version="1">MITLTTDFGLGSPYPAQVKAVILRIAAPGVDIVDVTHEVPAQDVVAGSYVMSVACPWFPPGSVHVGVVDPGVGTERRAVLLEAERGDFLVGPDNGLLIPLAEELGGIVRAFRILEDEVSDWEVSATFHGRDVFAPAAARVASGESPERFCEPLDPEELVEPPIAEPEVDDGHVRAQVWFVDDFGNIITNVLYDEVDLPETVTIRVSGESFEARHVHTYGEADSGDLVVLRSSSDHLEIAVVEGSAAELLGVSTADRLEILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX82</accession>
    <name>Q8TX82_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02007">
        <property type="protein sequence ID" value="AAM02007"/>
        <property type="gene ID" value="MK0793"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0793"/>
    <dbReference type="HOGENOM" id="CLU_052774_3_0_2"/>
    <dbReference type="OMA" id="THHILPR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="38"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="264"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="416" mass="46059" checksum="C6809D9854EE9DA2" modified="2002-06-01" version="1">MPFLLEGARQLKTIEVGVAYGKDPHAVVEDAIDQLTEDPNLGLVFFSPKHIEEIESAVGSLPEGCKVIGCSTAGELTPDGYTYGTVIVALISSPYLAVETHRITFEREDPKHAREIGRNLVLGAMEKLRNPGPCLDINFNAMLTSLSEGRPIRALPYFIIELVEGLVPAIDYYMDGINEVVQKYKFLEVYGGAAGDDLRLERTYVLEGRTLCPEGSAVVAFGSTALKIGGALECGFEPVYEDRFPVTKAVPEERRIVEFDGEPAADYYADVVGKPVEELDDSVFMWNPLGWEIFGREIIVREPAFVEDDGSMIFHSRSPAAGALIRLEPTEENMRETARRVTERAMRRADIRDPEEIALVLVFDCAHRDCDAQYDAIREVVGDDVPIVGFKTYGEHGQLESGPVGHFNQTIEVVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX92</accession>
    <name>Q8TX92_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01997">
        <property type="protein sequence ID" value="AAM01997"/>
        <property type="gene ID" value="MK0783"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0783"/>
    <dbReference type="HOGENOM" id="CLU_1192688_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="236" mass="24477" checksum="0BB07D6426936D6E" modified="2002-06-01" version="1">MMSIKMTIGATGAAEAASHGDVIVVVDVVNTSSAAEVALREGAVAVVGAAPDSAYRVLSGEHAAKYPFAETPEGVDPVERGREAGKIAVEEGCDVVLVVDGGEDNASLARKGVEDVGAEIREVVPNAGPRIKDVVDPAGKVFLFATATGGTLYDVAKTHGAPAVTFGTVVRRSFTRACVERALRLAGRYGAGVTIVVSSIFAPEDLDAGARIFEEACKVITESVCEERFEDVVSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX93</accession>
    <name>Q8TX93_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Isocitrate/isopropylmalate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">leuB_1</name>
        <name evidence="5" type="ordered locus">MK0782</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0782"/>
    <dbReference type="EnsemblBacteria" id="AAM01996">
        <property type="protein sequence ID" value="AAM01996"/>
        <property type="gene ID" value="MK0782"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0782"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.823"/>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="4">
        <location>
            <begin position="4"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="36282" checksum="12F3CD614BA715ED" modified="2002-06-01" version="1">MAYKIAVIPGDGIGPEVIEAALHVIEPLIDAEFVEGEAGDECAEKHGDPLPEDTLELCHEADAILFGAAGETAADVIVRLRQELDLYANIRPVRGFPGLRELTGEPYVRDDVDFVIVRENTEGLYSGIEGRFRDTAYTLRIITEEGTRRIAEVACDLAEERGSNTVTCVHKANVMRETCGLFREVCKEVVESRGLEFEEYYVDAAAMFMITEPERFDVVVTPNMFGDILSDEAAALVGGLGLAPSGNVGDRHGLFEPVHGSAPDIAGKGIANPFATILSAVMMLEWLGEDEAAEAVREAVGEAIREGVVTPDLGGDKKTMEVAEFVREAALNRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF0</accession>
    <name>Q8TXF0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0724"/>
    <dbReference type="EnsemblBacteria" id="AAM01938">
        <property type="protein sequence ID" value="AAM01938"/>
        <property type="gene ID" value="MK0724"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0724"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.765"/>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="3"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="309" mass="34650" checksum="A932EDC9B85A80CE" modified="2002-06-01" version="1">MILVTGGAGFIGSHVVEELVDRGHDVVVLDNFSVGCEENLREVRDDIEIVRADVTDPRAVERTFREYRPEAVIHLAAQVNVRYSMESPFVDARINALGTLNLVSLAAEHDVERFVYASSGGAVYGEPEYLPVDEEHPTRPISNYGVSKLAGEYYVRVYAERDGFEYVILRYANVYGPRQDPRGEAGVIPIFLLRAARGEPLTIFGDGEQTRDFVFVEDVARVTAEAVERGDGVYNIGTGRETSVNDIVNAVKAVTGVDVEVVYEDPRPGEVRRIYLDPSRAREELGFEPRVDLEEGIERTWEWIRRKIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF2</accession>
    <name>ACDD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit delta</fullName>
            <shortName evidence="1">ACDS complex subunit delta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhD</name>
        <name type="ordered locus">MK0722</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). Probably maintains the overall quaternary structure of the ACDS complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF2"/>
    <dbReference type="SMR" id="Q8TXF2"/>
    <dbReference type="STRING" id="190192.MK0722"/>
    <dbReference type="EnsemblBacteria" id="AAM01936">
        <property type="protein sequence ID" value="AAM01936"/>
        <property type="gene ID" value="MK0722"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0722"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.763"/>
    <dbReference type="HOGENOM" id="CLU_040403_0_0_2"/>
    <dbReference type="OMA" id="VMDPTTC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01135">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004486">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00381">
        <property type="entry name" value="cdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155113" description="Acetyl-CoA decarbonylase/synthase complex subunit delta">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01135"/>
        </source>
    </evidence>
    <sequence length="400" mass="44543" checksum="83ADA136E30C07CB" modified="2002-06-01" version="1">MAEDKGVKNMLEHLVKNLLVEDVEEIELRNVTIELDELELDLKTVAQTLPVEIWERILKPEVEEKEREVEVPEYEPPVEEYEGCVAEVQIGATRSDGGSRDRVVVLGGERAYFPFEEPRPNPPVVTFDVFDTPDVGIPGPIREELGDVIEDPVDWARTVVKRYGVDIVTVHLVSTSPKLHDAPVEEAMETLEDILDAVKVPIIVGGSGDPEKDVEVFVKAAEVCEGERVMLSSINEDMDFERVVEAAKEHGHVVLTFAPVDVNLMKSLNKKVLNRGLSKEDVVMDPTTCALGYGIEYTIDVMTRIRLAALKGDEHLQMPISSGSTNAWAAREAWMKEESWGPREYRGPLWEAVTATTVALCGADLLMMFHPWAVQVVMEAMEYMAEGRVTGDAYVTDVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG6</accession>
    <name>Q8TXG6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted AAA+ class ATPase with chaperone activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01922">
        <property type="protein sequence ID" value="AAM01922"/>
        <property type="gene ID" value="MK0708"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0708"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.749"/>
    <dbReference type="HOGENOM" id="CLU_029703_1_0_2"/>
    <dbReference type="OMA" id="WIVPLER"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="176"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="538"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="585" mass="65588" checksum="2444D4CE238A7AFA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ9</accession>
    <name>Q8TXJ9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">MK0674</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0674"/>
    <dbReference type="EnsemblBacteria" id="AAM01889">
        <property type="protein sequence ID" value="AAM01889"/>
        <property type="gene ID" value="MK0674"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0674"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.713"/>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="OMA" id="DYEEANI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="13">
        <location>
            <begin position="64"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="316"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="316"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM01889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="368" mass="42423" checksum="E7C4809608EC7E78" modified="2002-06-01" version="1">MAGDNEATEVDKRDIALLRALEGLSRAFEWVPEDKLLERLPMDYSELATRLEKLDSLGLIDYRYIPTYQTYAARMKERAYDTLALWDMKKHDVYERLGTIIGEGKEATIVNAKDPEDEWVAIKLHRYHAPEFRRIKKTLAYAAVKVRGEELRVDDHRIDVPRAKAQVEMKVLQRLHSKGFPVPGPRAINRHAVAMDMIEGHAPGIPAPLLAKIKVKNPEEALEVILEDYREIVLEGHYVHGDFSEHNILVTPDGELYYVDWPQAVPIEHPSAPKLCYRDLKNVIEHFRRKYRIRVPDPKEVYDEIADDLQSLMEEKKEEYERHKKAAERTLERFEESVERVEGKREGRKAGPTEDEEDRVPGGGDGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK3</accession>
    <name>Q8TXK3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0669"/>
    <dbReference type="EnsemblBacteria" id="AAM01884">
        <property type="protein sequence ID" value="AAM01884"/>
        <property type="gene ID" value="MK0669"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0669"/>
    <dbReference type="HOGENOM" id="CLU_093776_1_1_2"/>
    <dbReference type="OMA" id="QWKISIH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="179" mass="19048" checksum="36ACC2FAC68F40F4" modified="2002-06-01" version="1">MVVNPSTAEAATVILVGKKYGDVVGTALGLFFGVLLVILTAAVYIRVKGTDVFVSKRRDRLPLLTVGAIYHAIGALVMCKVGVTRPMVCLMLTYAIAAMAVAGVTRFWKISIHAASMGTVMGAIAWLGEWWAGIPWSIVTAVVCWARLKLNAHTGYQVAVGVAGGTLLTYVLMITLIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ4</accession>
    <name>Q8TXQ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Kef-type K+ transport systems, predicted NAD-binding component fused to an uncharacterized conserved domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kch_1</name>
        <name evidence="8" type="ordered locus">MK0606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0606"/>
    <dbReference type="EnsemblBacteria" id="AAM01821">
        <property type="protein sequence ID" value="AAM01821"/>
        <property type="gene ID" value="MK0606"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0606"/>
    <dbReference type="HOGENOM" id="CLU_714989_0_0_2"/>
    <dbReference type="OMA" id="WIISRAS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02829">
        <property type="entry name" value="3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75500">
        <property type="entry name" value="SSF75500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="116"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="375" mass="42437" checksum="157EEA0347373F5E" modified="2002-06-01" version="1">MVSVLRTLLRIPAVRRSLVVLSSILLTSTVIFHFLEGWPLLTCLYFTAATITTVGYGDVVPTTEAGRLLSVIVMFSGIGVASYALGDIIQLVFRGELSLAIKEDVLRKRIKEVENHIIVCGFGRTGSRVARLLSERYKFDVVVVDKDEEAYERAVYQGFPAVLGDATREDTLQEANVESARGMVVATGDDRTNVFVTLLAKNFRRDLHVVAVANSREGAKMMERAGADEVLFLYDCASRHIVRAALSPTMFRVTVRHSVDEISDVMWIIIGNGGVVQCVEYYTPPLKSPIRRDVVISSMDEVMRFVKSLEKYPERKRTLEDLYRVSENVHTYYVIVHNEEERERILRELDRRGYLVGVDLTIDEILQEIERLRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR6</accession>
    <name>Q8TXR6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01809">
        <property type="protein sequence ID" value="AAM01809"/>
        <property type="gene ID" value="MK0594"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0594"/>
    <dbReference type="HOGENOM" id="CLU_2447693_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="89" mass="9813" checksum="51398BC897681E32" modified="2002-06-01" version="1">MDSVVLSKKVKRIVEVRGDQLVIETQTGERCILKVDERGELRPVKFLVIKSGSYEVSVAGSAKAKAKARATPLILILPILLRLVRRSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT1</accession>
    <name>Q8TXT1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0579</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0579"/>
    <dbReference type="EnsemblBacteria" id="AAM01794">
        <property type="protein sequence ID" value="AAM01794"/>
        <property type="gene ID" value="MK0579"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0579"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.614"/>
    <dbReference type="HOGENOM" id="CLU_1567127_0_0_2"/>
    <dbReference type="OMA" id="GCARTNE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012356">
        <property type="entry name" value="UCP018781_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09885">
        <property type="entry name" value="DUF2112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018781">
        <property type="entry name" value="UCP018781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03271">
        <property type="entry name" value="methan_mark_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="148" mass="16579" checksum="5818CB2A93F438B6" modified="2002-06-01" version="1">MLVLARVFIHPPNSLILHDLVERFGHEPLSLPKEISKRVRDPEIDSPPMNVTPQDAKRGLKYAAVEVPSGVRGRLALIGPLIEKADAAIVVTGIEDFSFGCLGCDRTNEFVTYLVRRQGIPVLELEYPKSEEEAKAFVREIRDFLESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT4</accession>
    <name>Q8TXT4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0576"/>
    <dbReference type="EnsemblBacteria" id="AAM01791">
        <property type="protein sequence ID" value="AAM01791"/>
        <property type="gene ID" value="MK0576"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0576"/>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="129" mass="15114" checksum="72F10B6D6B8D01CD" modified="2002-06-01" version="1">MRPFLRPIVVELVQGTAELRPERVLSCVFGIKEDEAELYLKLLENSEEAPFTVEDVAEIINRSRSTAQKMLQSLVRVGMVERERETLPGGGRRYLYRPAPWEKVKELGLENLRIVCEEIERWFREFTPH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU9</accession>
    <name>FLPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">flpA</name>
        <name type="ordered locus">MK0560</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXU9"/>
    <dbReference type="SMR" id="Q8TXU9"/>
    <dbReference type="STRING" id="190192.MK0560"/>
    <dbReference type="PRIDE" id="Q8TXU9"/>
    <dbReference type="EnsemblBacteria" id="AAM01775">
        <property type="protein sequence ID" value="AAM01775"/>
        <property type="gene ID" value="MK0560"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0560"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.595"/>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="OMA" id="INMATHR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000148534" description="Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <sequence length="232" mass="26394" checksum="045BBD4F03020EB9" modified="2002-06-01" version="1">MVEIEIEPHEEFEGVYWAIFEDGRKKPATENLVPGHQVYGERLVEYDGKEYRVWEPRRSKLAAMIMNGMEYFPFEEGSKVLYLGAAAGTTPSHVSDIIKESGVEYCVEFASRMMQELIPVCEKRPNMIPILGDATKPHGYAPLVEQVDVIYQDIAQPKQAEVVADNAEAFLRPGGYVIVAIKARSIDVTKEPEEVFEDEERKLEERGFEVLEVIDLEPYERDHVGIVAEYHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV2</accession>
    <name>Q8TXV2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01772">
        <property type="protein sequence ID" value="AAM01772"/>
        <property type="gene ID" value="MK0557"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0557"/>
    <dbReference type="HOGENOM" id="CLU_129114_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007556">
        <property type="entry name" value="DUF483"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04467">
        <property type="entry name" value="DUF483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="189" mass="21982" checksum="3781660E6A9E319F" modified="2002-06-01" version="1">MNTLERVLTFLQDLRSHLDGTGDMPEPRTLAEFALQRLTPMDLDICINIVETELVLWEESGLHVRPALHPYVSERIGVYTLDDEEVGRFLGYPECCVEYFLEGHVRFDHDPDNVVVVTEGFVPCSPTCRRAHRVHLLEFDADPEPYRRLEGRLRTRLEKLGVLSYHSAYRGFYEVHVPKFEGVHLDRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY7</accession>
    <name>Q8TXY7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotide-binding protein related to universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0521"/>
    <dbReference type="EnsemblBacteria" id="AAM01736">
        <property type="protein sequence ID" value="AAM01736"/>
        <property type="gene ID" value="MK0521"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0521"/>
    <dbReference type="HOGENOM" id="CLU_049301_16_2_2"/>
    <dbReference type="OMA" id="IECDLIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="149" mass="15829" checksum="14B20ADD733FBAF8" modified="2002-06-01" version="1">MFERIMVPTDGSENAKQAAKAAIEIAKKEDATLIVIHVIPLWSPLGTKPSFTLPEEIVKEAEKIVNEIAEMAKEEGIDVETLVVESPSVVQGIVEEAKERDVDLIVMGTRGLSGVKGLILGSTTKGVLSRSPCPVLAIPPEGEGEGEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ8</accession>
    <name>Q8TXZ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01724">
        <property type="protein sequence ID" value="AAM01724"/>
        <property type="gene ID" value="MK0509"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0509"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.540"/>
    <dbReference type="HOGENOM" id="CLU_261589_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1268"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1188"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1193"/>
            <end position="1216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1299" mass="144992" checksum="BDC9CA3DFD1CBBF7" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ9</accession>
    <name>TRPF_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="1">PRAI</shortName>
            <ecNumber evidence="1">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpF</name>
        <name type="ordered locus">MK0508</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXZ9"/>
    <dbReference type="SMR" id="Q8TXZ9"/>
    <dbReference type="STRING" id="190192.MK0508"/>
    <dbReference type="EnsemblBacteria" id="AAM01723">
        <property type="protein sequence ID" value="AAM01723"/>
        <property type="gene ID" value="MK0508"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0508"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.539"/>
    <dbReference type="HOGENOM" id="CLU_076364_2_1_2"/>
    <dbReference type="OMA" id="FYAKSPR"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154404" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <sequence length="216" mass="23294" checksum="DBAC90CDEAAE1DD8" modified="2002-06-01" version="1">MVRVKICGITRPEDAATADEAGTDAVGCVVEVPVSTPRKVSAEHANEVFSVVSPFVSRVAVLMDNLEPIDRLEEATAVQLHGTEDPETCEELSELGLDVIKTFWVDQRGSVWLGEELIGDEVLAEYCEIVDAVLLDTKSAEGGGSGERHDWDASARLVRRLDVPVILAGGLNPENVREAVEKVRPYAVDTSSGVEKEPGIKDPEAIAEFVRATKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY13</accession>
    <name>Q8TY13_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0493"/>
    <dbReference type="EnsemblBacteria" id="AAM01708">
        <property type="protein sequence ID" value="AAM01708"/>
        <property type="gene ID" value="MK0493"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0493"/>
    <dbReference type="HOGENOM" id="CLU_1140604_0_0_2"/>
    <dbReference type="OMA" id="PVRQMIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016757">
        <property type="entry name" value="UCP019322"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09883">
        <property type="entry name" value="DUF2110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019322">
        <property type="entry name" value="UCP019322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="77"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="263" mass="29936" checksum="3C6975CCA1558251" modified="2002-06-01" version="1">MDVTILEKLYRNSGAEESLRTQVQSVISDFDAELEEIRKNFRAWAVLRIRGPEVEEAAEEIVEEFDTVRSLFDVSPGDVIYGRIVDLYRVGYGIYVDIGVVGPEVVDALVPLYRLREQLLDGEKASIRDIGRSFGFVENMPLEVEIVEVNEERGEIEARLTDEQVEYLLEMAADPYDRMIVAGITRKRLQRILNRAGFGRRVIRIERWGLLENEVVFDEGVDAPGVLAKIGPYLRGGEVELIYDDKFLERIGFIPSPEGPILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY63</accession>
    <name>Q8TY63_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01657">
        <property type="protein sequence ID" value="AAM01657"/>
        <property type="gene ID" value="MK0442"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0442"/>
    <dbReference type="HOGENOM" id="CLU_588778_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="464" mass="51772" checksum="F019F650B44CD0C8" modified="2002-06-01" version="1">MDAAILTVSVLSLCVDLNKQADRLETHLPPFRLSSPYPTPFRYVLARPESIEVHPPMDPEGELRIEVLPSGSKVLFDSREYGARTVVEFRGPYLMVTRLPVVGLPRDLPRAAVFFSDGRRNYALISYRDVATGRAYVALCEIALNLHELVRVPVPISLDLGERLSEFTLYDNGTFSAKFEAGGGELVVRGSLRPPGLSVEWDGEGELIGEKRPILVPGTSWALLNKCPVIVFGQGTNDLLWPDREYLVWRTLTSYRSELEDVVEEIVKRSKRSIDLGPALLCAYSTLAGLTVDTLSQVRPPRLWSGPWAWLGAVVETVVGIPVLEWGVLLPIVTRTGELIGTVAPTVDRSGVVTGIYWLTVEWYEAWLRRVIMRTCGLSWWETGMLWGALDALFNSLKERSLNPHDLMRRLTFHVAASLSPVLEVALYAENAEDTGGDVITYYSSILAAMVRWPEQSLPLQRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY69</accession>
    <name>Q8TY69_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anthranilate/para-aminobenzoate synthase component II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pabA</name>
        <name evidence="2" type="ordered locus">MK0436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0436"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="EnsemblBacteria" id="AAM01651">
        <property type="protein sequence ID" value="AAM01651"/>
        <property type="gene ID" value="MK0436"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0436"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.465"/>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="OMA" id="TEHGHAM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="21236" checksum="E5BF2A986EE64065" modified="2002-06-01" version="1">MRRLVLINNKDSFVYNLYHLFASYDLDLKVVSNKVPLSRLERLRPDGLVVSPGPGHPERDAGVSVPAIRRFAGEIPILGVCLGHQCIGVAYGAQIRRAKRIVHGKTSPIEHDGSGILSGLESPFQGMRYHSLVIEESSLPEELLPCAWSGDDGELMAVRHADYDVYGVQFHPESFMTEGGDRIARNFLELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY74</accession>
    <name>Q8TY74_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01646">
        <property type="protein sequence ID" value="AAM01646"/>
        <property type="gene ID" value="MK0431"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0431"/>
    <dbReference type="HOGENOM" id="CLU_2010113_0_0_2"/>
    <dbReference type="OMA" id="DELIECR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="78"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="123" mass="14169" checksum="F80361D7E95FB817" modified="2002-06-01" version="1">MTIAEFVAVGVLTLAIIVAIALIVRSREVPEGEGLSMLRDKISLTTRSDVITKKVDELIECRVEELIDSWGLATTEDVEKVEKRVDALDRKLNELEQRFNEFRNDIRRKIERLESRLRELSSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC1</accession>
    <name>RRP41_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp41</fullName>
            <ecNumber evidence="1">3.1.13.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp41</name>
        <name type="ordered locus">MK0381</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic component of the exosome, which is a complex involved in RNA degradation. Has 3'-&gt;5' exoribonuclease activity. Can also synthesize heteromeric RNA-tails.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family. Rrp41 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC1"/>
    <dbReference type="SMR" id="Q8TYC1"/>
    <dbReference type="STRING" id="190192.MK0381"/>
    <dbReference type="EnsemblBacteria" id="AAM01596">
        <property type="protein sequence ID" value="AAM01596"/>
        <property type="gene ID" value="MK0381"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0381"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.407"/>
    <dbReference type="HOGENOM" id="CLU_063514_0_0_2"/>
    <dbReference type="OMA" id="KGKGQGW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11366">
        <property type="entry name" value="RNase_PH_archRRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00591">
        <property type="entry name" value="Exosome_Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011807">
        <property type="entry name" value="Rrp41"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02065">
        <property type="entry name" value="ECX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139982" description="Exosome complex component Rrp41">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="239" mass="26515" checksum="204BA6A9F85D343D" modified="2002-06-01" version="1">MEERPERLISEDGLRLDGRKPDEMRPLKIQAGVLKRADGSAYLELGANKIVAAVYGPRELHPRHKQKPDRAVVRFRYNMAPFSVDERKRPGPDRRSIEISKLSKEALEPAIFTEYYPRTAIDIFVEVLQADAGTRCAGISAASVALADAGIEMRDLVAACAAGKVEGKVVLDPMYYEDGYGEADVPLAMMPKEGKITLLQMDGDMTPGEFKQAVKLAKKGCKIVYKEQRRALKEKYGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF3</accession>
    <name>Q8TYF3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ABC-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01562">
        <property type="protein sequence ID" value="AAM01562"/>
        <property type="gene ID" value="MK0347"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0347"/>
    <dbReference type="HOGENOM" id="CLU_598017_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019195">
        <property type="entry name" value="ABC_ATPase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149">
        <property type="entry name" value="PTHR38149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09818">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="467" mass="52925" checksum="90A30B9299E804A1" modified="2002-06-01" version="1">MRGLEEKIRTLQLPTDVRWCPKRMTDRSRGRRWYAVRGSDAVRVVLPVRVSRRDFKKVPKGAGDAAAHPIIEEIKRRSREEPDLYPLSGKLLDHKKVRKLTEPCTVVRLHGFRTPPLWMPGKSNRVLRRSREVLLTVRMLFPPDWSEERVLDGVYAALDALREVVREIPRKEILRTCDVVLDQRELRERLERGEIEHRGKRVVALIGDGARPARRLTDVRRHHRIAGPKPEPHIPFEVPEDPDADLVPLEIELPRTGERKRFLPIYEGELFAIAGANAQGKTTLVNAVEAGQDDHAPGDGREYVITVRRTAKAEAGIKRMNGEDVSLFFRELPPGYEGTPENVRGLASGSMKMAAEIQRALEEGAKILFIDEDKAAVNLLVPGVLTKELEGIRTLVEVRDEITSRGVTIVAATGVLDDFTAAADRAIVMEDHRPKPLNLREFRARLARYYLERARRYRSDAEGPEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP0</accession>
    <name>Q8TYP0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0253"/>
    <dbReference type="EnsemblBacteria" id="AAM01470">
        <property type="protein sequence ID" value="AAM01470"/>
        <property type="gene ID" value="MK0253"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0253"/>
    <dbReference type="HOGENOM" id="CLU_1149816_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TrmB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="241" mass="27444" checksum="E68AC54347307C48" modified="2002-06-01" version="1">MSLLKHLSELGLSEREARVYLYLVRAGEANAREVSEGVEIPYSKVYSVLRSLEDKGWVEADRSARPTTYRPVSPDVAVKRALEQELNRIRRELEEHAKVAVRKLSEIYRAERETVARTYHGKSARETLAEVLRSSEDIACIVHLGRNLPRWLLESLRRMEGLMILRAKEGSEVADELEPDVKAPIDIEPRNTLIVLSADRREFFLGKFGEGDYLLSLEEPILAQGIHDAVTRACKSRSRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT2</accession>
    <name>Q8TYT2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Diverged homolog of ATP-dependent DNA ligase (Eukaryotic ligase III)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01427">
        <property type="protein sequence ID" value="AAM01427"/>
        <property type="gene ID" value="MK0210"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0210"/>
    <dbReference type="HOGENOM" id="CLU_665007_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="413" mass="47668" checksum="270CA6FDB979B6C8" modified="2002-06-01" version="1">MSDTETIETPSGERPLLPPKARLTFSRTMPAALPETVEHTVRLYITELEHVSSNESLRIEEKKDGTNVRVVLGRVLESELGEPVIAHTRSWVRVEEVEEYVQSVLDPSEHSLTIVEGELLPLSLFGTSVFELWNETLDYIDYHVRLMSRYTLNPRRKRVPRAWLGKLFDRLSKVRRHVRTLYTIQRRLPDVKLADDDEFQRLHEEIERLTAIAHDKDYVPEDAVEILRDILSVLDDLAAYEPEDSEPVYYVYELDMYDGEITIQKPKIRQYELLADMIDGDRIKPVPSTLCDLGEAEQVARELMNRVEEEELEGLVIKPEKEVPGVPHARKVRAEWYLRGMKLGSRLSLKGFAGAAEKYARNEVRRALAAIETSYLYRALEALAEGDLRRASRIVKEAEEVTDRMSEWDDPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU8</accession>
    <name>Q8TYU8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Predicted EndoIII-related endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nth_1</name>
        <name evidence="12" type="ordered locus">MK0193</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0193"/>
    <dbReference type="EnsemblBacteria" id="AAM01410">
        <property type="protein sequence ID" value="AAM01410"/>
        <property type="gene ID" value="MK0193"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0193"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.194"/>
    <dbReference type="HOGENOM" id="CLU_012862_3_3_2"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="11">
        <location>
            <begin position="54"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="10">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="233" mass="26610" checksum="A94CBA5C3DC0A665" modified="2002-06-01" version="1">MFPSVPSLRISPGVGPGRRVNPVMEIVKHYEDHPIMKKLKVRRDPFRALIQAIISQRTRDDVTDRVAERFLRKFKTPKDVAEVNLKDLVETLRDAGLYRQKAKMIKECCERILADGLDLEEIVQKPTEEARRELMRLPGVGPKTADVVLLFAGGHDVCPVDTHVARVSRRLGLTDSKEYFEVQEAVHEMVPEGERGKAHLALIQFGREICRPRKPQCELCFVRRFCPYGGEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW5</accession>
    <name>Q8TYW5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0176</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01393">
        <property type="protein sequence ID" value="AAM01393"/>
        <property type="gene ID" value="MK0176"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0176"/>
    <dbReference type="HOGENOM" id="CLU_1656875_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="159" mass="17919" checksum="57AA06E94144AE78" modified="2002-06-01" version="1">MKSWYGEVLETVDDAENFFERELRRMHKVELEEVPKVVFCAFDVIEGVKHEFEPEKYLYKPGAIPAGAQALTLDIMTDDHREDAVYIGEPGPEVSRDRWAGRVVSVLARLLPECVEVGLLEDVRELEGVRVRIGKFTLLNYVTGAALALSGAEVELEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY1</accession>
    <name>Q8TYY1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0160"/>
    <dbReference type="EnsemblBacteria" id="AAM01377">
        <property type="protein sequence ID" value="AAM01377"/>
        <property type="gene ID" value="MK0160"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0160"/>
    <dbReference type="HOGENOM" id="CLU_795996_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="348" mass="38256" checksum="FF3A9BF3F3036124" modified="2002-06-01" version="1">MIGAIVIGLIFLAAGGGAYYFFVYKPYVEQLEKLRAQKLKELNTYFTGPLAASPTRTKLQQQILSAETPEQLQAIDVVGAATVEWRRYLAKQIKMNQKKGRVELVTPEGPQLLTVRDALNKIRMMGVDELMKVQVKRPETVLIAIWADPNKTGPIKVGDRVTLSITNWALKKIAKVKNKEAYKGPNQISGAIVRYIMLIKGGLPDNFKIDLVSASALETMYRTGSSDLALRKVVFPAGLVTGRSYAVGGGTGVTYTLKNYPGLNTRVTSPHLSPVALVDLRDLVKAMAVERARRGGGFIRQLMSIEAREGIRSWENLLVIVEIPKDAVQPKVLIASSVKGGIWILPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY3</accession>
    <name>Q8TYY3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sortase (Surface protein transpeptidase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">srtA</name>
        <name evidence="2" type="ordered locus">MK0158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0158"/>
    <dbReference type="EnsemblBacteria" id="AAM01375">
        <property type="protein sequence ID" value="AAM01375"/>
        <property type="gene ID" value="MK0158"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0158"/>
    <dbReference type="HOGENOM" id="CLU_1631663_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05830">
        <property type="entry name" value="Sortase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005754">
        <property type="entry name" value="Sortase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023365">
        <property type="entry name" value="Sortase_dom-sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042003">
        <property type="entry name" value="Sortase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04203">
        <property type="entry name" value="Sortase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63817">
        <property type="entry name" value="SSF63817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01076">
        <property type="entry name" value="sortase_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="162" mass="18477" checksum="54FB65C42E707068" modified="2002-06-01" version="1">MRLRYLIPGILLLAPSTTYLGYLAAKEVAYFMYHESVSIPDCELVIPKLGLRERINTTSPDYGVYYEIMTPPPGKKGITVFYGHRTLFGSPFLHLDELKRGDKVIVYWFGSKYVYVVYDKVVVSPDYVIDPDASNKDELWLVTCTPLSTARERLIVKCVRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY4</accession>
    <name>Q8TYY4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Archaea-specific RecJ-like exonuclease, contains DnaJ-type Zn finger domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0157</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0157"/>
    <dbReference type="EnsemblBacteria" id="AAM01374">
        <property type="protein sequence ID" value="AAM01374"/>
        <property type="gene ID" value="MK0157"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0157"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.157"/>
    <dbReference type="HOGENOM" id="CLU_018957_0_0_2"/>
    <dbReference type="OMA" id="IGYGPDF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="137"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="752" mass="83864" checksum="301281949AC8D2BA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY6</accession>
    <name>RL35A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L35Ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl35ae</name>
        <name type="ordered locus">MK0155</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYY6"/>
    <dbReference type="SMR" id="Q8TYY6"/>
    <dbReference type="STRING" id="190192.MK0155"/>
    <dbReference type="EnsemblBacteria" id="AAM01372">
        <property type="protein sequence ID" value="AAM01372"/>
        <property type="gene ID" value="MK0155"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0155"/>
    <dbReference type="HOGENOM" id="CLU_100745_3_1_2"/>
    <dbReference type="OMA" id="SKENQHN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="L35A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_L35A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_L35Ae_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="PTHR10902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000192810" description="50S ribosomal protein L35Ae">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00573"/>
        </source>
    </evidence>
    <sequence length="90" mass="10053" checksum="79A42CE0A873114F" modified="2002-06-01" version="1">MSEVKRGVIVNYRMGRHTQDPRQCIIEFEGVESRSEAAQLIGKEVIWKHPETGKVIRGKVVDTHGNNGAVRVRFERGLPGQALGTEVTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ03</accession>
    <name>Q8TZ03_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">comB</name>
        <name evidence="7" type="ordered locus">MK0138</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0138"/>
    <dbReference type="EnsemblBacteria" id="AAM01355">
        <property type="protein sequence ID" value="AAM01355"/>
        <property type="gene ID" value="MK0138"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0138"/>
    <dbReference type="HOGENOM" id="CLU_070028_0_1_2"/>
    <dbReference type="OMA" id="VEIADSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="220" mass="23590" checksum="438534385458997E" modified="2002-06-01" version="1">MRVASGLEPQEGYEIGVVVDALRASSTIVTALALEAEAIVPLSSPEELKRVDGPTIGEQHGKKIDFADYGNSPTDLLRHAEEIEGETLYMVTTNGTDTILRAAEVHEEVLIGSLLNASAVASKLSGDTCFVEAGHRGMLAVEDTYTAGYIARLAGGEPADGRTRAAMEMARGLPAEEVFKGSRTGHVLEQRGRLEDVEFCARVDEFEVVPVYEDGMVVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ42</accession>
    <name>Q8TZ42_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted carbamoyl transferase, NodU family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01314">
        <property type="protein sequence ID" value="AAM01314"/>
        <property type="gene ID" value="MK0097"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0097"/>
    <dbReference type="HOGENOM" id="CLU_014411_2_0_2"/>
    <dbReference type="OMA" id="RTEMDYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbam_trans_N" evidence="2">
        <location>
            <begin position="75"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Carbam_trans_C" evidence="3">
        <location>
            <begin position="348"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="515" mass="58007" checksum="0D4C63E06CF8A697" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ88</accession>
    <name>Q8TZ88_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01267">
        <property type="protein sequence ID" value="AAM01267"/>
        <property type="gene ID" value="MK0050"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0050"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.50"/>
    <dbReference type="HOGENOM" id="CLU_907968_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="306" mass="35290" checksum="2FD50BC86B42FDD3" modified="2002-06-01" version="1">MIRAIATVDENASYIAFVETPSANEKLLDVMSREKWRELGMFVLDHHGQLSEEPSAVAQAYEIVKEHTEFPLVRKVVIEPPVDADMVAAVLLLKQGPVLPEEDVEVLDKIDRFGKHYPSDEELESAEYALAIMQAVAEVVKKPYPDNPEELKETFKKALRTVERVVDDEKYREKLASKWLEEFRKSVREAEESTEVIEEGEVEVGNDRIRWCAAISEAQNAFTYLYREGYDLVVLYSPVMKRVTVGLADPELPIDLRELFEYLNEHHREGWGGRKNIGGSPKNYEIDEEEFKDIVSFIHEWLLRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC6</accession>
    <name>Q8TZC6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpp1A</name>
        <name evidence="4" type="synonym">rpl12</name>
        <name evidence="6" type="ordered locus">MK0004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0004"/>
    <dbReference type="EnsemblBacteria" id="AAM01221">
        <property type="protein sequence ID" value="AAM01221"/>
        <property type="gene ID" value="MK0004"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0004"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.4"/>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="OMA" id="EYIYAAM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_L12/P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_L12_arc"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="58"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="105" mass="11112" checksum="FB8B3CCEF135850F" modified="2002-06-01" version="1">MEYIYAALLLHAAGQEINEDNLRKVLEAAGVDVDDARLKATVAALEEVDIDEAIEEAAVPAAAPAAAAPAEEEEEEEAEAEEEEEEEEEEEAEEEAAAGLGALFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P58860</accession>
    <name>PYRE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">MK1072</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58860"/>
    <dbReference type="SMR" id="P58860"/>
    <dbReference type="STRING" id="190192.MK1072"/>
    <dbReference type="EnsemblBacteria" id="AAM02285">
        <property type="protein sequence ID" value="AAM02285"/>
        <property type="gene ID" value="MK1072"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1072"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1126"/>
    <dbReference type="HOGENOM" id="CLU_074878_2_0_2"/>
    <dbReference type="OMA" id="MKAYQRQ"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000110780" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="115"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="183" mass="19600" checksum="610C9A96A449B1F5" modified="2002-06-06" version="1">MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPDGDVLAGPELGAVPLVSVLSVKAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATTGGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGVRLSSVLTADDLRGLRDAESTARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ8</accession>
    <name>Q8TGZ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0295"/>
    <dbReference type="EnsemblBacteria" id="AAM01512">
        <property type="protein sequence ID" value="AAM01512"/>
        <property type="gene ID" value="MK0295"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0295"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.299"/>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_2"/>
    <dbReference type="OMA" id="NPELRWR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004573">
        <property type="entry name" value="rRNA_ssu_MeTfrase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00563">
        <property type="entry name" value="rsmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="5">
        <location>
            <begin position="164"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="256"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="449" mass="51639" checksum="653F62F252568554" modified="2002-06-01" version="1">MTEFWARCYALAAKSLIEVMRTKSYPIQYVLRKTLSEYDDKTQSVTRSMVYDVLRRYGTLERVVEDVSRSELPPLERALVMVAANEVLYEGKHPAPVTDSATRAAKELGLNHRRVHGVVADLEEYERPEPEDEIDRLCLEYHHPRWFVEKFGALLDEDELKELMEVHNEPPEYYTFRVNTTAADVEDVLREFEEHGFEVERGEYVDYCIRVKKGQPLRLEELECWREGHIVPQDEAAALVTEILNPQPGERIADLCAAPGGKTTHIAQLTEDEAEILAVDVSRVRLRRLERFAERMVFENIEVLRADVRRLGRNPRYVGKFDRVLLDPPCSSLGTLRSDPDVKWKIGPRDIRELALKQRQLIRAAARLLKPGGVLVYSTCTITPEENELVVSEAIKRDRLRPVDVRSEFEFLHPPLRVDGVSSSLEAGRMWPHVHGTTGFFVAKLKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS1</accession>
    <name>Q8TUS1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1683"/>
    <dbReference type="EnsemblBacteria" id="AAM02896">
        <property type="protein sequence ID" value="AAM02896"/>
        <property type="gene ID" value="MK1683"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1683"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1848"/>
    <dbReference type="HOGENOM" id="CLU_076869_2_0_2"/>
    <dbReference type="OMA" id="QMWFILE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B3_4" evidence="1">
        <location>
            <begin position="58"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="218" mass="24338" checksum="ADAA017F51EAEE5D" modified="2002-06-01" version="1">MRVEVDPEVNDRGLRYAYVRVEGVDPGADASELVDRVTARLRERFDLDDLKDDPIVRAYRDYLWSIDVDPTKVRPAGEALLRRALRGNFPRINAVVDSYNLASAEYRVPISCFDADKLTGDLIIRPAESGEVMVDIAGERMELDDRFVVVADEDGPVSVSPYRDARRTAVTDETERVLLLAHGVPGVEVEHLVEALKTAVRYLREGAEAESASRIVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT3</accession>
    <name>Q8TUT3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GTP:adenosylcobinamide-phosphate guanylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1670"/>
    <dbReference type="EnsemblBacteria" id="AAM02883">
        <property type="protein sequence ID" value="AAM02883"/>
        <property type="gene ID" value="MK1670"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1670"/>
    <dbReference type="HOGENOM" id="CLU_098907_0_0_2"/>
    <dbReference type="OMA" id="GMGEKPC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="22359" checksum="594E1850E56280D6" modified="2002-06-01" version="1">MMAGGRAKRMGGVEKPAVEVAGKPLLVWVLEALQDCCCVDRITVAVSRDAGVTRSIAERLGAEVVVTPGDGYVHDLRFALESVGTPALTVTADLPCLTPDIVGLVIAAWAAVPEPSLSVWVPRSLIVKAGLSLWRRFESTVGNVRAVVVGLNVVGGLRKTDEFKLLLDEPRLAYNVNTWHDLRKVEGVLRSCPREERAQGLQALKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV5</accession>
    <name>Q8TUV5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02861">
        <property type="protein sequence ID" value="AAM02861"/>
        <property type="gene ID" value="MK1648"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1648"/>
    <dbReference type="HOGENOM" id="CLU_583451_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="468" mass="51790" checksum="AFC9C3586D4A4E78" modified="2002-06-01" version="1">MTGGKRGWNPASIDPRLYQRTGFLIVATLNLVIVGLVAAVGIKVIVEKHYPMVVGAIAIPIVVAPLLLPLLYLLRVLKREKLVVVGEDRVLIIRGKDVTEVPVDEITGISVDFEFKRRRRKMIILTRSGHVELPFHSKRLMRDLQRLLEERRASEVDKETYARAGADRVATVLCKMVELGRSPVLHEAQGSVKAVWPGDSVELGREDGWAPRWMYWFLCVASLPVKAAKRSLREDPVDTVIAAEEAAKGQSLASSFNKILRTGPIVSALLVFLAVVRGVLKYWGPKALGVFLFTAAPWILATSVLSFLLNNRVKTVELHGVRAFFSLVAMVSIFSGLGTCLHGSLAETAMLRKSLPPSAHLTAVIELVIAMVSLVPLVKTLRWVDPRGAVRWCMALRATIREDRVILFSVVFALLSVFFAGSAASPGLWWVTFPIATATPVLWTVYFLSWSKMAREVVSKVWIPESES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV05</accession>
    <name>Q8TV05_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Metal-dependent hydrolase of the aminoacylase-2/carboxypeptidase Z family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1596</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02809">
        <property type="protein sequence ID" value="AAM02809"/>
        <property type="gene ID" value="MK1596"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1596"/>
    <dbReference type="HOGENOM" id="CLU_1025316_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="271" mass="28907" checksum="7B561EAA39351E36" modified="2002-06-01" version="1">MIAVLLVLLALPTSGCCEAVWTVYDWGSKGNILKNPVLVQHAPHGPRSEVVYRHSVEGTVVFEFRGGPGPAVLLTAGVHGDEVWTVIALLKLLDALSPTSVIGTVYVIPAVNPAGLAANSRLVDGVDPNRTADIPGSLTWHLVRFALSHRVKYWLDMHCGSGVPRQGALLTDEESEFVDELARSSGFVPLVKSAPRGSIRSVARRFGIDVITLEVPRDAGPSGVERAYHAALAFLRLTGALRRGQSRTPASGSANVTVPVLPAIVPRRRLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV06</accession>
    <name>IF2P_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">MK1595</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV06"/>
    <dbReference type="SMR" id="Q8TV06"/>
    <dbReference type="STRING" id="190192.MK1595"/>
    <dbReference type="PRIDE" id="Q8TV06"/>
    <dbReference type="EnsemblBacteria" id="AAM02808">
        <property type="protein sequence ID" value="AAM02808"/>
        <property type="gene ID" value="MK1595"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1595"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1756"/>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_2"/>
    <dbReference type="OMA" id="FRQSKPA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137302" description="Probable translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="8"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <sequence length="598" mass="66605" checksum="080BB46FB486FB27" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV12</accession>
    <name>Q8TV12_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATP-dependent carboligase related to biotin carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1589"/>
    <dbReference type="EnsemblBacteria" id="AAM02802">
        <property type="protein sequence ID" value="AAM02802"/>
        <property type="gene ID" value="MK1589"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1589"/>
    <dbReference type="HOGENOM" id="CLU_057102_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016677">
        <property type="entry name" value="UCP016817_carboligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016817">
        <property type="entry name" value="UCP016817_carboligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="122"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="385" mass="42758" checksum="B08258E6A8632942" modified="2002-06-01" version="1">MPRKHSRSSKSCKLVFVGVVNRQFSELAIELEFEVGAVQFYDPDDQPGDVISYVREGPGRLLPRFRPPDDQWLIRALEELQPDVIVPTSEFAVEAAFRTSKKLGAEYAGNEPEVVNVAADKLELFNRLSDVLPMPETSEDPSELGVETLVEKPVRGAGGLGVRKVKLDEADPRPGVIFQEFVPGRHVSVTFVSDGSDVRVLSVNDQLIDLRSEYSYKGNLVPSPYHLVPSVREEARRVCEVLVDELGLVGLNGVDAVLNKYGLHVIEVNPRPTAVTECLARVSGDNPIRLHLQAFDGSLPERWKIRGWSCKRVVYAPNTVRVPYLSWTRDRPRPGTVIPRGEPVCSVIASSSTPSGARSMAKRLERVVVNRLERVSSTPPTEGWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV14</accession>
    <name>Q8TV14_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted transcriptional regulator containing DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK1587</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1587"/>
    <dbReference type="EnsemblBacteria" id="AAM02800">
        <property type="protein sequence ID" value="AAM02800"/>
        <property type="gene ID" value="MK1587"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1587"/>
    <dbReference type="HOGENOM" id="CLU_1521881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019264">
        <property type="entry name" value="DUF2179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10035">
        <property type="entry name" value="DUF2179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="8">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="176" mass="19623" checksum="45A1ED67F0EF87E0" modified="2002-06-01" version="1">MKKIFRNGGFGGPFLMRSGINRDVIEVLMDVYGLTERQATVSVMRSEGMSYREIAEELGITVSGVRNHLEQARMKMKVDNDFQIARIIGKLEMSLGPALVIAIVPVDKSEDVINRLIQEGEGVTEMTGRGGYTGEEQSVLFIITEDEKKVREIIEEEVGREVPMFVMRAPAAFPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV28</accession>
    <name>Q8TV28_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02785">
        <property type="protein sequence ID" value="AAM02785"/>
        <property type="gene ID" value="MK1572"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1572"/>
    <dbReference type="HOGENOM" id="CLU_1458203_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="185" mass="20396" checksum="40716F27B0E71F30" modified="2002-06-01" version="1">MNVSTTLIFTTISPGTSPVTFIRTLPFTSNLLFLTSISIVSLFENLNAKVFVVTLSPSGSLILSTRTLYFKCFELILANGTLVPKPLLTISSIIKCSDFILFIFKSNPISILTSYSPSEAVKAILSFLLNSYVSVVVSDLAFINTLFSVGTILIVSPMFNLLKIKRSFSFRLRLATLIRTMFAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV29</accession>
    <name>Q8TV29_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02784">
        <property type="protein sequence ID" value="AAM02784"/>
        <property type="gene ID" value="MK1571"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1571"/>
    <dbReference type="HOGENOM" id="CLU_1025315_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="271" mass="30264" checksum="C2581A642D5862E9" modified="2002-06-01" version="1">MKVTGDVPGDIVVNIRVVDTFIHGKIKLWCYYDGCLKTRNIETFGSVVVISDDGDWTIINLEDRILLYDSDNGIFEIKLENEYINVRRWDVIKRKWGSVRKVRVNDFDFEDIVPNKYRLAIVGMMEIQLPGNNKKILYSILKDLASIKCEVTENGCSVVAESDGTPPLKLGDIENPIKGIEANIDEEGRIIVSASDKPVYKPLYTGMETVTLRVSPIPVEVKVNGENVPIIGLPVISDLSGTLQVAITKDTILAGFIDGEGAFRWLGVYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV30</accession>
    <name>Q8TV30_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted Co/Zn/Cd cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mmt1</name>
        <name evidence="7" type="ordered locus">MK1570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02783">
        <property type="protein sequence ID" value="AAM02783"/>
        <property type="gene ID" value="MK1570"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1570"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1732"/>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_2"/>
    <dbReference type="OMA" id="IEMHLIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="293" mass="31836" checksum="CAB317BA3707DC87" modified="2002-06-01" version="1">MSGVEERLQEVRRGALIGIYGNILLSVLKIAIGHIASSWALVSDGVHSLGDTVSSVMVLLGAKVARKPPDARHMYGHYKAEALAGLGVSMMVTFTGLLLLWESLMRLRNPEPPTSTLPLVVALGTVAAKEGMYRYTLRLAERVGSSALKADAKHHRSDALSSVAASVGIAGALLGYPWADPGAALVVSLMIAHMGLELCWESVHELMDVAPEDELLERVHEIVENTLQDVDFDVELIAVRGRKMGPAYHFDVFVAADPEARLRELDTVRKRIVEGIRREISAAELVTVEFVPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV32</accession>
    <name>Q8TV32_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02781">
        <property type="protein sequence ID" value="AAM02781"/>
        <property type="gene ID" value="MK1568"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1568"/>
    <dbReference type="HOGENOM" id="CLU_2191040_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020380">
        <property type="entry name" value="Uncharacterised_MJ1658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17393">
        <property type="entry name" value="DUF5402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="108" mass="11959" checksum="CDFEFDE3353C8EDF" modified="2002-06-01" version="1">MSVVNLREEARKLEEELSGLLGRPVRVPVVEVFVRRCGCRGIVLYVKGLTLDDIEVLGELLSDALSRLGDGCPVAELRRIGPEVRSLMLLEACDEHPPDEDPSLLPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV38</accession>
    <name>Q8TV38_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted flavoprotein related to choline dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1562"/>
    <dbReference type="EnsemblBacteria" id="AAM02775">
        <property type="protein sequence ID" value="AAM02775"/>
        <property type="gene ID" value="MK1562"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1562"/>
    <dbReference type="HOGENOM" id="CLU_759945_0_0_2"/>
    <dbReference type="OMA" id="CDAKWIP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="364" mass="40221" checksum="B839591E72AEC00A" modified="2002-06-01" version="1">MKVYIVGAGPSGCWIARRVHEMGYDVTVITHPENRLQSERFGHYRVYYGEGLGGSARYSMGNFWFWGPEGLRRPFERVLRDMTCEVRRTPDSVVSNLDIEFEEAAEDVGLEPERMPKSVHFDLCDGCGECLECPRNAKWVPQDELPEGVSVLRDKCIQLEFSGGKATAIVLRSQTVELETDDIVVLCAGCPGTPRLLERSGIDADGPLFVDAYIHVVSDLEAEPGIQMPIVVDMDEYILSPHRTSAYEGKYSIMVKIADELSGDVIGREKPISVRETELFAEGCAVAGEIISRMGGRVRAVTEPSGAHPGGTLSDRVDDRYALEGYDNVYVVDANVLPEPLGKPPVGVILAMAEDFCDKLRRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV41</accession>
    <name>Q8TV41_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02772">
        <property type="protein sequence ID" value="AAM02772"/>
        <property type="gene ID" value="MK1559"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1559"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1719"/>
    <dbReference type="HOGENOM" id="CLU_1253593_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="220" mass="25474" checksum="5E58B830B98A92A2" modified="2002-06-01" version="1">MDVLDELYLRFREAVEVAGHSEIPSQFLQHWVARTLEDALEVVGDLCPGDVMLEVGVGSEVGTFQDRPRRIDILVRDRVIIETKRPGELNSGGRLEEARDQLLRYFAYLVKERDVRPEDVLAVLTDGKIVVYYEVEKEELVQEGPKKLDRSEFRRMIKTILRRIGEDPRPLELSRTSVELNPTAVVQWIETWKEVRELLERVKKELTGDGRSERPRGPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV45</accession>
    <name>Q8TV45_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02768">
        <property type="protein sequence ID" value="AAM02768"/>
        <property type="gene ID" value="MK1555"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1555"/>
    <dbReference type="HOGENOM" id="CLU_1529240_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="175" mass="19325" checksum="695D9BAFFD1AC73B" modified="2002-06-01" version="1">MMTTATAGAASIYLGGKLASGKEAWKSEGKPLVELGTVLYATGLRLSDSPIPENVALVAFRGLEHVHVYKREIDLNVPGFAQAVKKSVERPDDAFVEALKSMSPTLRYCVDSLLNHDRKLVERFSRDILRVMKGALGSSPTVAEILDVIDEHVEEDEGWIPWNPVRALLEILGWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-05-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA3</accession>
    <name>COFD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="1">2.7.8.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofD</name>
        <name type="ordered locus">MK1489</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + H(+) + oxidized coenzyme F420-0</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVA3"/>
    <dbReference type="SMR" id="Q8TVA3"/>
    <dbReference type="STRING" id="190192.MK1489"/>
    <dbReference type="EnsemblBacteria" id="AAM02702">
        <property type="protein sequence ID" value="AAM02702"/>
        <property type="gene ID" value="MK1489"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1489"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1647"/>
    <dbReference type="HOGENOM" id="CLU_055795_1_0_2"/>
    <dbReference type="OMA" id="DLDTVMY"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="PTHR43007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000145773" description="2-phospho-L-lactate transferase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <sequence length="310" mass="34882" checksum="1EA80A7C3DCD8975" modified="2002-06-01" version="1">MNLRLTVLSGGTGTPKLLRGLRELEADFSVIVNTGEDDEILGLYVSPDVDTVLYTLAGIVNDETWYGIKDDGFRGHEFLERLGVDEPLRIGDADRALKQYRTYLMREKGLKLSEAVDEIRRRLGIKWKVYPMTDDRVTTIVETDEGDLHFREFWVERGGKPPVRGVRYEGAEEASPPPDAVDELLRADVVLIGPSNPVTSIGPILSISEIRHIVREKPVVMVSPFIGREPVSGPAGKLMRAVGFEPSVRGLVEYYREWGVEPDVLIMDERDDVELPEGLEVVRTDTLMRDEKDSVRLAREVLRIVEELVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB0</accession>
    <name>Q8TVB0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator of the LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1482"/>
    <dbReference type="EnsemblBacteria" id="AAM02695">
        <property type="protein sequence ID" value="AAM02695"/>
        <property type="gene ID" value="MK1482"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1482"/>
    <dbReference type="HOGENOM" id="CLU_039613_6_3_2"/>
    <dbReference type="OMA" id="IEGPCHH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33435" checksum="B08CD2753D95F5D1" modified="2002-06-01" version="1">MSDPRFSYFRTFREVVRQKSFSKAAEALGITQGTVSNQIASLERFFDARLFVRTPEGVELTEEGEIALEAIETVLDAVERAKDKIAAVSKEPSGKVRVSTSTVPGGYLLPGSVKEFRSEYPKVDVIIRVCDSREATEHVLSGDADVAIVGTDAFVTRKSVEVVPIASEELVVILPPDHELADAMEVSIDDIVGEPYVNRESGSGTRREVEKYLKSHGLGFEDFKIVEEVGSTEAVITSVSQGAGISIISERAAKRAESAGLIRIARLEDRPRRFFYALKSDKPLHASATEAFWEFLLSEFRGKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB4</accession>
    <name>RPO10_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo10</name>
        <name evidence="1" type="synonym">rpoN</name>
        <name type="ordered locus">MK1478</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB4"/>
    <dbReference type="SMR" id="Q8TVB4"/>
    <dbReference type="STRING" id="190192.MK1478"/>
    <dbReference type="EnsemblBacteria" id="AAM02691">
        <property type="protein sequence ID" value="AAM02691"/>
        <property type="gene ID" value="MK1478"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1478"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1634"/>
    <dbReference type="HOGENOM" id="CLU_143122_2_1_2"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="PTHR23431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="SSF46924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000121350" description="DNA-directed RNA polymerase subunit Rpo10">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <sequence length="72" mass="8423" checksum="6345896E76B89DC3" modified="2002-06-01" version="1">MIIPIRCFTCGRPIAHLWEKYVELIEEEGMEPGEALDELGVDRYCCRRMFLSHVDLLEESLPYTPPRLGMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC4</accession>
    <name>Q8TVC4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1468</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02681">
        <property type="protein sequence ID" value="AAM02681"/>
        <property type="gene ID" value="MK1468"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1468"/>
    <dbReference type="HOGENOM" id="CLU_1465100_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017098">
        <property type="entry name" value="UCP037052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09869">
        <property type="entry name" value="DUF2096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037052">
        <property type="entry name" value="UCP037052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="184" mass="21302" checksum="051DF05BC1F237B0" modified="2002-06-01" version="1">MKAQWVALNEVVSELRARGEEVPGEVLENLRTARVILHHYEFDPHTSARTLGKAHKYLDRAQRALARICESHPDLLDKLVEWPKRVKELAARFREDTARSKFEPMPNRPVPRAGDGFARVRLPEPIEVERLQDVCEFAGVIVEMKEVDVVEVFGDRDRVGTALKELRELPESWKKMLEKRKSNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC6</accession>
    <name>Q8TVC6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02679">
        <property type="protein sequence ID" value="AAM02679"/>
        <property type="gene ID" value="MK1466"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1466"/>
    <dbReference type="HOGENOM" id="CLU_1346415_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026898">
        <property type="entry name" value="PrsW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13367">
        <property type="entry name" value="PrsW-protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="203" mass="21203" checksum="A646412665BAF16A" modified="2002-06-01" version="1">MILTPVIAVTGVLLGLLYAPISVVIAFLVETVLTSGLSGPYRLVIAAVVVAPIVEELSKGLGFLVVPRVLAGALRLASRFPVIGPTVGTAAKVVRECGRRPVGTVTVAATTALGFGSMENVFYALVGLKFGLLGPIIVGFVRTFTSLPIHVIATSSFGLLYGSLRRRWLGFAVGYSTAISIHAAFNFAAVYARYKATHGLIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE4</accession>
    <name>Q8TVE4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing PIN domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1448"/>
    <dbReference type="EnsemblBacteria" id="AAM02661">
        <property type="protein sequence ID" value="AAM02661"/>
        <property type="gene ID" value="MK1448"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1448"/>
    <dbReference type="HOGENOM" id="CLU_107892_1_0_2"/>
    <dbReference type="OMA" id="DEMIINY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="130" mass="14863" checksum="C8CBA8A0B57DC570" modified="2002-06-01" version="1">MTIRVVLDANFLMIPHQEGVDVFSELDRLLGSYRPIVPRQVLEELERVKRAATGRDKIAARVALSLVDAKGIEVVDVKGRDGDEAILNLARRWDRVYVGTRDKELKKRLWKLGVPVITLRQRTHLVIERG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF2</accession>
    <name>LEUC1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit 1</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase 1</fullName>
            <shortName evidence="1">IPMI 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC1</name>
        <name type="ordered locus">MK1440</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVF2"/>
    <dbReference type="SMR" id="Q8TVF2"/>
    <dbReference type="STRING" id="190192.MK1440"/>
    <dbReference type="PRIDE" id="Q8TVF2"/>
    <dbReference type="EnsemblBacteria" id="AAM02653">
        <property type="protein sequence ID" value="AAM02653"/>
        <property type="gene ID" value="MK1440"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1440"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1596"/>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076870" description="3-isopropylmalate dehydratase large subunit 1">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <sequence length="418" mass="45202" checksum="FABA735A9BD51C72" modified="2002-06-01" version="1">MGKTMAEKILSRASGEDAEAGDIVVANIDVAMVHDITGPITVQRLEEMGVERVWDPSKIVVLFDHQVPADSVEAAENHKIMREFVEEQGIEHFYDVREGVCHQVLPEKGHVRPGDVIVGADSHTCTHGALGAFATGIGSTDMAAVFATGKLWFRVPETYRVEITGELPEGVYAKDVVLKVTGEIGADGATYMAIEYHGEVVREMSVSDRMCLCNMAIEMGAKTGMVPPDEKTLEYVKKRAGTEGRPVEPDPDARYEAELTLDVSDLEPQVAKPFSPDNVVPVGEVEGIAIDQVFIGSCTNGRYEDLKVAAEVLEGEEVHDDVRLIVIPASREVYHRTLKDGVLEVLHEAGALICPPNCGPCLGGHMGVLAEGERCVATSNRNFPGRMGHRESEVYLASPATAAASAIEGEITDPRPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF5</accession>
    <name>Q8TVF5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Selenocysteine lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">csdB</name>
        <name evidence="2" type="ordered locus">MK1437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1437"/>
    <dbReference type="EnsemblBacteria" id="AAM02650">
        <property type="protein sequence ID" value="AAM02650"/>
        <property type="gene ID" value="MK1437"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1437"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1593"/>
    <dbReference type="HOGENOM" id="CLU_003433_2_2_2"/>
    <dbReference type="OMA" id="PVCLRYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="1">
        <location>
            <begin position="17"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="394" mass="43626" checksum="0CE42DB7639460A5" modified="2002-06-01" version="1">MGLDEIVNGFPLKEEWVYLDNAATSLKHERVISAMERVLREFGVNVGRGAHPPGECATEEFERARDIVASFLGVEPECLAFTLNTTHSIHYVLASIRWKKGDAVVTTALEHHSNLAPWLRFSEVLGFEVEVVGFDRETGEVDMAELESVVDDNTRLIAITHESNALGSLQPVDEILELAEEVGAYVLLDAAQSLGHMDHDWSRYHFLAAPGHKGLLGPHGTGILYVREDVMEELELRLLGGGSTDYVTRDLEVVPREPPLSFESGTPNLPGVIGLAEGVKILEEVGLNRVERRIRKVTRRILNGLEELEGVEILAPEAERKTIVPFLVDGVDYAEVGKKLGERNICVRTGRHCASLVFERLGLDGCVRASVAFYNDIEEAERFLEVVEEIARGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF6</accession>
    <name>Q8TVF6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase subunit of an ABC-type transport system, contain duplicated ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1433</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1433"/>
    <dbReference type="EnsemblBacteria" id="AAM02646">
        <property type="protein sequence ID" value="AAM02646"/>
        <property type="gene ID" value="MK1433"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1433"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1589"/>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="283"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="320"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="532" mass="60592" checksum="45C30368F05012C3" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH4</accession>
    <name>Q8TVH4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted SAM-dependent methyltransferase involved in tRNA-Met maturation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1415</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1415"/>
    <dbReference type="EnsemblBacteria" id="AAM02628">
        <property type="protein sequence ID" value="AAM02628"/>
        <property type="gene ID" value="MK1415"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1415"/>
    <dbReference type="HOGENOM" id="CLU_025402_4_1_2"/>
    <dbReference type="OMA" id="VVMHNKS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="193" mass="21796" checksum="20D5E66115BA574A" modified="2002-06-01" version="1">MDLDRLKVSSFERVTRPIEPKDSGLITERLGLLPGHRVFESGVGSGFLTASMARIVYPEGEVVGIEIDTRKLEKARENLEQLGKVYEKSVTLKHGDAREYLEGLEDEFDAMVLDLPEPDRVLEVGLDALKSNGKVAVFCPFFEQVRAVWQVLEDRCTWLEAVELIERKLQVEKRGIRPGRTLGHTGFIVFGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH7</accession>
    <name>Q8TVH7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NAD-binding subunit of the Kef-type K+ transport systems</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">kch_2</name>
        <name evidence="6" type="ordered locus">MK1412</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02625">
        <property type="protein sequence ID" value="AAM02625"/>
        <property type="gene ID" value="MK1412"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1412"/>
    <dbReference type="HOGENOM" id="CLU_2857106_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="4">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="283" mass="32032" checksum="5058E27A6440BD79" modified="2002-06-01" version="1">MRGGLREFLYDIDQRRWLRAVRDHVVVCGFGRVGAQAAGRLRAHGFDVVVVDTSKERVERARREGSVFPTVEGNLTDRRTLERAGVDRARFVVVCTDSDETNVYVTLLVRKLNPDARVIAVARDPENADLLPHAGADEVVDAYRVAGEEAVERVLAEHSFTVTVRHDLDEVEKEWRTIAVENGGTILDVRFHVPESPEEPIVKELPVESPEDLQRRKELLGTSEEFRSMAEALHELCRGAHSHRVFVADPSDKERIVKELEKLGFLIGVDLSHEEVLDRTFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI3</accession>
    <name>Q8TVI3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Terpene cyclase/mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02619">
        <property type="protein sequence ID" value="AAM02619"/>
        <property type="gene ID" value="MK1406"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1406"/>
    <dbReference type="HOGENOM" id="CLU_596678_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="458" mass="51684" checksum="8C3B515050B5C940" modified="2002-06-01" version="1">MGILRPLVLALVTVALSTAPGSAITAEQIQECARAAHDWLKSQQYTEEDVGKVIEYKDRSGQKATYEVKPEDVGAFPMSYKSDVTYYCLPNLHTYVVMDTLLTAVEVGYPVDKDMVLKALQYMWNVKGKYQRKEDPNGEWAYWARIKDGKEIPSAGLTAKFALPFFRARQAGIVPDDKWNEYKPFLEKVINWLREGPNLQKEDKGYCWIDYPDRPGGAHGITDVTSYCLRMLLAAGTSPDDELILKVVEWFLNNQDEKGNFYTDPRDTGCPYPLYTTGHCRAMIALCDWLEAVQRAGKEDELKDLVERVKNALKKAIDYLLSLRDPETGMWGRGSLSEYPPDYGSTASALVALLRCAKLGLIDPNHEAIVKALEVLHGQYLEAKRLRLPFYWYFIRSVWSPELRYRSQTLATSYALAAFALAEKLGVGKGVVKKRKIFPVTPAVVALALAASLLTRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ0</accession>
    <name>Q8TVJ0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rIO1_2</name>
        <name evidence="12" type="ordered locus">MK1399</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1399"/>
    <dbReference type="EnsemblBacteria" id="AAM02612">
        <property type="protein sequence ID" value="AAM02612"/>
        <property type="gene ID" value="MK1399"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1399"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1554"/>
    <dbReference type="HOGENOM" id="CLU_955149_0_0_2"/>
    <dbReference type="OMA" id="HEERVYR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="11">
        <location>
            <begin position="67"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM02612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="291" mass="33054" checksum="83BEAD15EF041C17" modified="2002-06-01" version="1">MEQLGRALLETVQKDDPEPFLRALEETEVDPEDAERFLEALRIQREKGRISDETLEAVEDALFKVSESEEREIPEPDPLAEYAGVDRILGTIMTGKEADVLLAERDGEKVALKVYRAHTGYEERHEERVYRLEDGEVRRIERGDAALREFSRLRRAYEAGVRVPKPYDARPGLIVMEYIPGEPLYRAPDLDDPGSVLEDLLDQVVRLAVDAELVHGDLSAFNVLVGDDGVPYIIDLSEAVKVKEPGAFETLRRDVKNLVSFFERKYGVSADVDEVVERVRRGVYGTDRGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK0</accession>
    <name>Q8TVK0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="11">ASA dehydrogenase</shortName>
            <shortName evidence="11">ASADH</shortName>
            <ecNumber evidence="7 11">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">asd</name>
        <name evidence="14" type="ordered locus">MK1389</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="7 11"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1389"/>
    <dbReference type="EnsemblBacteria" id="AAM02602">
        <property type="protein sequence ID" value="AAM02602"/>
        <property type="gene ID" value="MK1389"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1389"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1544"/>
    <dbReference type="HOGENOM" id="CLU_049966_1_0_2"/>
    <dbReference type="OMA" id="CEEEMKM"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000319">
        <property type="entry name" value="Asp-semialdehyde_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01103">
        <property type="entry name" value="ASD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="13">
        <location>
            <begin position="5"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="11">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM02602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="354" mass="38304" checksum="FC680FD617562075" modified="2002-06-01" version="1">MAKVRVGVLGATGIVGQRFISLLADHPWFELEAVTASPRSAGKTYAEAAKWYLEEPMPEDVAELTVLETDPKEVEREADVDLVFSALPSDVARDVEPAFAEAGFAVASNASAYRMEEDVPLVVPEVNPDHLGLIDVQRDERDWDGFIVTNPNCSTIQMVLTLKPLMDEYGIESVVVSTMQAVSGAGYAGVPSMAIIDNVIPYIEGEEEKMETETLKILGELDGDRVEFADVKVSASCHRVPVLDGHTEAIFVATEKEADPEEAAEVLAGFRGVPQEKGLPSAPERPVVVREEEDRPQPRFDRDVDGGMAVVVGRIRKDPVFGGLKYVCVGHNAVRGAAGASVLNAELLVEEGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK9</accession>
    <name>Q8TVK9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator containing a DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1380"/>
    <dbReference type="EnsemblBacteria" id="AAM02593">
        <property type="protein sequence ID" value="AAM02593"/>
        <property type="gene ID" value="MK1380"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1380"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1535"/>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="OMA" id="KMAGEIT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="33"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="27481" checksum="37306AB654DCEF79" modified="2002-06-01" version="1">MNHPRGVISLPRSLVLKRIVGDIAASEHPGKVMRKWRKVFHASQVEVARRMGVSPSVISEYETGKTKAPRVDTVRKFVEALIEIDEERGGNIVSALENVLFSEELLVTLIGIGEFPYPRKLEEVYEAIEAEPVVHHGNVDVFGYTVIDSVKTILEVPARSLIRVYGECPNRVLVFTRIDRGRSPMVAIKAAGVKPSAVVLHGIDKSEVDDIGIKIAEVEGINLATTTESISRISKRLKELTEVQPGDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL5</accession>
    <name>Q8TVL5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02587">
        <property type="protein sequence ID" value="AAM02587"/>
        <property type="gene ID" value="MK1374"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1374"/>
    <dbReference type="HOGENOM" id="CLU_1840581_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="139" mass="14801" checksum="8E0808731A75957E" modified="2002-06-01" version="1">MPVYKSAGVEYKPSLYVIPMCFQGDLIAGELAVATLLFVIIVGSLSLISLRNAIHARDKGRVVLHIGMLTFLASFTYAAFRMVQSSLFVPVGPPSPRTVAVPPAIVSGNEINMVFVASLIGLGLVVVGAIASMTRRIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM2</accession>
    <name>Q8TVM2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ligT</name>
        <name evidence="4" type="ordered locus">MK1367</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1367"/>
    <dbReference type="EnsemblBacteria" id="AAM02580">
        <property type="protein sequence ID" value="AAM02580"/>
        <property type="gene ID" value="MK1367"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1367"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1519"/>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigT_PEase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="LigT_PEase" evidence="3">
        <location>
            <begin position="97"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="21392" checksum="68B6BD09AC245F24" modified="2002-06-01" version="1">MSRFRAFISIDIEDEEVVNRIVEVQERLKASGADLKLVEPENVHLTLKFLGDIPESRVTDVVNAMEKAAETVEPFTMRLKGIGVFPNPNYVRVVWIGVQEGSDETKAMAAVLEQELGRMGFRRERKDFVPHVTVARVRSGRNKGRLIEAIRELSNVEVGEVEVDRIRLKKSILRPQGPEYHTVEEVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP5</accession>
    <name>Q8TVP5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02556">
        <property type="protein sequence ID" value="AAM02556"/>
        <property type="gene ID" value="MK1343"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1343"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="203" mass="23788" checksum="D875930A27E52F16" modified="2002-06-01" version="1">MVGSRIPIPSDPVERIRALRVLREVYRRGKKPSLEVTYRTVNGSTCGPYYVARWRCDSRFKHGRTLYLGKPENESVSFVEWLVSLDRNEVLKLARHLMRNLRSVLKTLLTEISNLPYKRARRVLARGLALTFDARPSNSPRIRDVLEELPDRLESFLIRTLGGWPAHYSSHLNKIIRSRRKSLDGKHEIPDVLLELERWKLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ4</accession>
    <name>Q8TVQ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted membrane-bound metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1334</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1334"/>
    <dbReference type="EnsemblBacteria" id="AAM02547">
        <property type="protein sequence ID" value="AAM02547"/>
        <property type="gene ID" value="MK1334"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1334"/>
    <dbReference type="HOGENOM" id="CLU_795998_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="348" mass="38958" checksum="F08AA050B8DE14F3" modified="2002-06-01" version="1">MRARTHVLLTYLLTAPFLGVERAITASLSAILPDLDHPNSLVSSALYPLELLTGRDLRLALSRRLRHRGILHCPWPWGALAYLLWATGHPNAALVPLGGFLHCLEDAFTTMGVPVMWRREGGSWRSVRLSLTGLPSDTWDAILPPLALAVTWALFLLNPAEFHATHLDENPYLERAYWTSRAVLLRPFLHTVEGLNREALRVSGYGRLPHVRAVLRYDYGPREVSGLWTPAGWVRGDDGRWYPPPTHGYSLRSLSYERAAYRVGTVRCWDAPEGALVLRAVLAYEGLSPVALERLAREAVLLGFRVRHTDRVGGHLVLEGGGLAIPVLHRLPRPAWCRVTFLPSRSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR9</accession>
    <name>Q8TVR9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK1319</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1319"/>
    <dbReference type="EnsemblBacteria" id="AAM02532">
        <property type="protein sequence ID" value="AAM02532"/>
        <property type="gene ID" value="MK1319"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1319"/>
    <dbReference type="HOGENOM" id="CLU_762101_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="363" mass="40540" checksum="CC05F1C494E1BC8A" modified="2002-06-01" version="1">MSGLETVEVRLEVLTPLHVGVPREEELVRGLDYRVDGNRILVVDFTTLPPERAERVLGALEGGDHAEAEGYLEGAEVLREVELRTGGVPDRFLPTYRPTRVDDPWIPGTSVKGALRTGLLLAALKRLDGLGTDLSTLTDRLPRNPKKAAVALDNRLRATVFQGGTARRSYRRKPNRGWPEVFPHADVLRALKVEDARPVGKIRTAVYRAELHPKGRPLDAREFVVSGTFELELGTDHGELKRVLDTWDRVELPRLPKPVFRFLRDVRSGLRDFWEETLENPETVLERAREGWREGLRALSERFRVDVPDDADVQLGWGGGRLVKTALPLAGRVLRGPLDQFAPQTVKLVDGEVPGLVRVVEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT4</accession>
    <name>Q8TVT4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein specific for M.kandleri, MK-14 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1304</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02517">
        <property type="protein sequence ID" value="AAM02517"/>
        <property type="gene ID" value="MK1304"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1304"/>
    <dbReference type="HOGENOM" id="CLU_673715_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="408" mass="43925" checksum="CF802BA70E35023B" modified="2002-06-01" version="1">MKVTTRTTRGMEPPVPPRFVSTRTGTARPPYTYRKAYTAGRIRGSAGVEGAPDIGERSTLLALRWLELFSDVWIVILGLTTAIVFFVFGLLPSLLLLFVLLLISAPMTAPVRFVLSTIPLIVVLWSARLADREVIVLSVILLIAALWSARSAGRKVVREASRIVEEAEDIVGVVFRAEGTPDKLDRRLSGLLKVPDRDLSGGKGTQFVVGSRGYIKGCRGALREALKIVGSGDALREFAEEGSLVTFHVTLVIPPTSRYEFKTEHDLRGIAIPLGRRSEDRAPIVALLGVGRRGSAEDWTLAFGGVVPLGETFSELHPHEGRTDDGHGSRRHRDHDGDHNGWCAGGDGLGDYHWWDSDESGGIFESIVDSIAEIAESMEGGWGNGGWFGDGGDWGDGGNGGDGGNGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV0</accession>
    <name>Q8TVV0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02501">
        <property type="protein sequence ID" value="AAM02501"/>
        <property type="gene ID" value="MK1288"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1288"/>
    <dbReference type="HOGENOM" id="CLU_870479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="36037" checksum="188CA7E281023B13" modified="2002-06-01" version="1">MLPYLVLGLVLALAPASGEPLIQGSLSKDLDYLHEYFQKQVLPKWRDEPHVIADHPARRFCYLPGKDVFVTGAPEVRVEPGSIRMEVQLGRRIIQVARDVQGPAVVVVPGVSYYKVMALAGQYVCEVGEVILAGRPPRFAAVLKRDDQVIAREDLGELFLADVEYWVYSDRVVMRVLGFRPEGGWSRYREGEPLAITVTFDLGGGRILVDRVKVTDAALVERMRELSRSLEGPVLGSVLRTGSGVEPLFVDFEPREGDPLVRFIERVFGMYEQGASEEELREVMSEHPNVEFLFPEKGGGTTSGIPVWVLPLLPVPLAGRRDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY4</accession>
    <name>Q8TVY4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02467">
        <property type="protein sequence ID" value="AAM02467"/>
        <property type="gene ID" value="MK1254"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1254"/>
    <dbReference type="HOGENOM" id="CLU_3113062_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="50" mass="5728" checksum="D49291FCF244045E" modified="2002-06-01" version="1">MSGDYELLHKRRERVQRLRVERHEPAHTDPRRLGGAGLAVRPSILPVTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW51</accession>
    <name>Q8TW51_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02398">
        <property type="protein sequence ID" value="AAM02398"/>
        <property type="gene ID" value="MK1185"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1185"/>
    <dbReference type="HOGENOM" id="CLU_2067825_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="118" mass="12162" checksum="875F5F9A5D508A1F" modified="2002-06-01" version="1">MRILTLLVAALTVLGAVSATTYYCPVTGRYFDDSTARGQHCGAFVDKNGDGYCDNLQVLSEETSDSSAETTSTSSTNTSEQQSSTASTTSSTTSSTAPVGLVAVIAGLVVAAVLLRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW80</accession>
    <name>Q8TW80_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-24 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02369">
        <property type="protein sequence ID" value="AAM02369"/>
        <property type="gene ID" value="MK1156"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1156"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1235"/>
    <dbReference type="HOGENOM" id="CLU_1763882_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="147" mass="15719" checksum="63CC16D762EEE5FF" modified="2002-06-01" version="1">MGCLPTVLAALAAIFVVGGVATATDDTYPHHYVKFRIEPKIQSVEVPSEVVAEGGTAEVPVKVTVTWTGNYPCTIKRTPTRMTFAVDGNVVNTQMITPPYKVGDTRTVSTTLRLTPGEHEITVTVRFPNLCSTGSPVEPTRRASGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA2</accession>
    <name>Q8TWA2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02347">
        <property type="protein sequence ID" value="AAM02347"/>
        <property type="gene ID" value="MK1134"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1134"/>
    <dbReference type="HOGENOM" id="CLU_975250_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="285" mass="31181" checksum="EAC3F042C60E7F3A" modified="2002-06-01" version="1">MPEYPKPGTVREAVYVLSVPGDRATLDLVFDVPPWARVLDLYLSAGSRVLGAGMVPRSAYSGLDHYVPAPELPGFVRLELAGERTERWSSDRFPATDPWLRGLLKRHGLRWTVVRYFLPRNGLFWARCEVPLLDGFVVWPAPFVGGYYVYGGVTEARLVPEGGPDRRLTFGPLPPLSDLDATLVLVLDGPPAVVVSSRGFHRTVLVFRPVGASPEAYRYAADPLRVALGLLGGAVSLLVRPTVPKALAALLVLVPVAGLDYRWPWLTGWATLGPAVLFLCLLHVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA4</accession>
    <name>Q8TWA4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1132"/>
    <dbReference type="EnsemblBacteria" id="AAM02345">
        <property type="protein sequence ID" value="AAM02345"/>
        <property type="gene ID" value="MK1132"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1132"/>
    <dbReference type="HOGENOM" id="CLU_123170_1_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="135" mass="15256" checksum="79AE884C41FFB59B" modified="2002-06-01" version="1">MDVDTEELVRMAEWKLSEAERALEEGPESPAVAEAHQAVELLVKAALRELDVEPPRTHDIRRLLGVLTTELLDLGEGDLAVEVRRIAREHRDTLASLLEGYFSLFEPPFEGDAEEVVRAAGKVFEDLRRILEKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB4</accession>
    <name>Q8TWB4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-23 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02334">
        <property type="protein sequence ID" value="AAM02334"/>
        <property type="gene ID" value="MK1121"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1121"/>
    <dbReference type="HOGENOM" id="CLU_856887_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="35929" checksum="F89AA8EC187E6BFB" modified="2002-06-01" version="1">MRLNAFVVYARPKGPSLTVDSGVLERLGAGCREVEVGSRLDLPLKLVEDGLVEPGRVTIYVPTTWGSRAPARALSVVKRMETVGYEVHVLVGTEGGSGRLPEGFPGEVRVENVKRDLSGILKLSDDIFGLFWSEGSLLLDLGWARDPGPRSDLAILTVSTLVALKVVENTSGERPLERTVMMMEVDRRLVDASELLHALMVALDLAYFPIAPDLGRTVREGIEELLEGLEGSEREELRKAADAFERLSGSVLDAFELGRVEIPDRPGILRELLEYRLRGLSPAMIDGEFPRRAKRMLKKVRRRLKRGDGRPDDTSSERPKRPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB9</accession>
    <name>Q8TWB9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-8 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02329">
        <property type="protein sequence ID" value="AAM02329"/>
        <property type="gene ID" value="MK1116"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1116"/>
    <dbReference type="HOGENOM" id="CLU_497509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="547" mass="61892" checksum="703545867A162A5D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD2</accession>
    <name>Q8TWD2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1103</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1103"/>
    <dbReference type="EnsemblBacteria" id="AAM02316">
        <property type="protein sequence ID" value="AAM02316"/>
        <property type="gene ID" value="MK1103"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1103"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1158"/>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="OMA" id="MNLDQKQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="51"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="303" mass="34218" checksum="3F88AF2CA48FC12F" modified="2002-06-01" version="1">MRCTRCGERNAEYERPYAGDAVCGRCLVELVRDRVFREIRRWRWFRPGQRIVVALSGGKDSSLALRLVTEYVEPLPEVEVIALTVDEGISGFRDACLRAAERVADELDVEHEVVSFEEEYGFALEEVVEDVDVSACTLCGVLRRRLLNRRARELGADVVVTGHNLDDEAQAALMNFVKADLAQLARLHPEVRPEDDLIVPRVKPLRGVPEREVRWVAEELGLPFHADPCPYARFSVRSFFREILDEMEERLPDVKFGLVRALDRAGPVLAEAFLEEGLGRCERCGEPAAGKLCKACELLERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE9</accession>
    <name>Q8TWE9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ferredoxin domain fused to pyruvate-formate lyase-activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK1086</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1086"/>
    <dbReference type="EnsemblBacteria" id="AAM02299">
        <property type="protein sequence ID" value="AAM02299"/>
        <property type="gene ID" value="MK1086"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1086"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1141"/>
    <dbReference type="HOGENOM" id="CLU_690041_0_0_2"/>
    <dbReference type="OMA" id="CNLRCPQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="151"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="390" mass="43064" checksum="64297CE8BDEDAC7F" modified="2002-06-01" version="1">MVRKVRLVGCRHCGYCSQVVDCPVGDPLWNREDCVGCGACVPACPYGARRLVEVEEGPITVRVDGEEVEVESRWVEGALREIGHDVDAPCGVGGCYACAVRLNGEVVPACNSKLTEGDEVETEDVDGKIRVVSGFQPHPVGGVGTPVELKDKPGYVEAACFAHGCNLRCPQCQNHSIAFGAAMGARMRPEEAARLLVGTAREYGVNRVAISGGEPTLNREFLVEFVRKCREYGGPDLRVHVDTNGTVLSPDYVDELVEAGMTDIGIDVKGFRPETFAEVAGIDVKGAAEYVDGVLRILEYLADEYLEEVFVGVGIPYNPELVDKEEVFALTDWLYEHLGEDVQVCYLDYRPEFRRRDLPLPKYEDMVELEEYARSLGFRRVHAQKVSVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF1</accession>
    <name>Q8TWF1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Queuine tRNA-ribosyltransferase, contains RNA-binding PUA domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1084"/>
    <dbReference type="EnsemblBacteria" id="AAM02297">
        <property type="protein sequence ID" value="AAM02297"/>
        <property type="gene ID" value="MK1084"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1084"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1139"/>
    <dbReference type="HOGENOM" id="CLU_526420_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF5591" evidence="1">
        <location>
            <begin position="241"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="517" mass="58844" checksum="098FC71B1F99FE1A" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG5</accession>
    <name>MDH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Malate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.299</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mdh</name>
        <name type="ordered locus">MK1069</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-malate + NADP(+) = H(+) + NADPH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:10824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.299"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.299"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.299" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG5"/>
    <dbReference type="SMR" id="Q8TWG5"/>
    <dbReference type="STRING" id="190192.MK1069"/>
    <dbReference type="PRIDE" id="Q8TWG5"/>
    <dbReference type="EnsemblBacteria" id="AAM02282">
        <property type="protein sequence ID" value="AAM02282"/>
        <property type="gene ID" value="MK1069"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1069"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1123"/>
    <dbReference type="HOGENOM" id="CLU_045401_1_1_2"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046554">
        <property type="term" value="F:malate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000113486" description="Malate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q60176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9YEA1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="317" mass="34609" checksum="D2AC04B7C0B6C26E" modified="2002-06-01" version="1">MSKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDAEITIGCERDDYVDADVIVMTAGIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLVVTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIAKHFNVHMSEVHTRIIGEHGDTMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLVRTILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSVKVVKEATREAMEAISER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI2</accession>
    <name>Q8TWI2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ELP3 component of the RNA polymerase II complex, consists of an N-terminal BioB/LipA-like domain and a C-terminal histone acetylase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">elp3</name>
        <name evidence="14" type="ordered locus">MK1052</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + carboxymethyluridine(34) in tRNA + CoA + 2 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ELP3 family.</text>
    </comment>
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    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
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        <property type="reaction ID" value="UER00055"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
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        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="PTHR11135:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="89"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        </ligand>
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        <location>
            <position position="114"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001658"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM02265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="549" mass="62745" checksum="B85EB671F47D3FE3" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI4</accession>
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        <submittedName>
            <fullName evidence="2">Predicted metal-dependent hydrolase related to cytosine deaminase</fullName>
        </submittedName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="49"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="370" mass="40840" checksum="3E1341579A1CCEF8" modified="2002-06-01" version="1">MKDGVLTAEAGLVGRDLELLSPITIEIEDGRVRRIEEGREPGADDDFDVVLPAPFNAHVHAADWAFRHAGLGMPLEKVVAPPDGLKHRLLEKVGERELEVSIRDFLETSLSFGCPGVADFREGGVEGLKLGLRAAEGFPTYVPMGRPKALDDPERVEEELRVLSELTEFVGIPDVHLPDDVLEAVRDSGLRVYVHANENWRSVRECVREHGATEIERAVDLLEPEGIVHCVVLTDRDRELLEELDPVVILCPRSNDYYRLGNPDPRRLRGLRVLLGTDNGMSVEPDPWAEAYHAWIRTGLSPLGALRALTVEAASVFDLPILEEGRRLSAVGLKGLPLPETVLGSRQRLAAHVLQLIKTSRVHVLLGAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP1</accession>
    <name>Q8TWP1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0991</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
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        <lineage>
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            <taxon>Methanopyrales</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02204">
        <property type="protein sequence ID" value="AAM02204"/>
        <property type="gene ID" value="MK0991"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0991"/>
    <dbReference type="HOGENOM" id="CLU_1232777_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="25807" checksum="721B24FE1C37007E" modified="2002-06-01" version="1">MELARECAPELKSGRVKEIYKTLKEMAERVPLKEYSEARCSLMVWVRAEEELPTNSIAQYVTSDTRYTFEVTVRQADSTSRPRIVEVILERHEARSRGPLDELKVHGSASVDEPVERRGRSLIGALKIALRYLREYDVEREWKVWKVTNKFREMVQKVTSLGSDEPGILVMEDSKVGTEYDGLFEYWVALKSGSIALSAVRRVLEVDRKLSLYGVHVEVPDARW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV7</accession>
    <name>Q8TWV7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02137">
        <property type="protein sequence ID" value="AAM02137"/>
        <property type="gene ID" value="MK0924"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0924"/>
    <dbReference type="HOGENOM" id="CLU_873231_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="318" mass="33750" checksum="3E31751510976CC0" modified="2002-06-01" version="1">MGTAGRFGGWWRSRIRPPKRPRRTGEVRRLSWVLEMIRTLLCMYVGVLIASYLGSSEACTRSLLRVLKPVVGSGLYGHAASLSALSSSVALYAAQDKVNSTREAVGLLLLLAFPTSVATVLQFYLPVVVPAVGPAGLLMIGISVVGALLTSAFGRVLLPGREDGDGPSKITLVRNPWRTAHSVFLRVGPAVALAMVAVKLGEGYGVTEWVERFLHPLTRSVGLPSDASLVVFGCLINVAVGAALGADLWASGKLRMEDLALALAFGRALSLPRINLQFLFPPAVTFLGKKGLLGATVRTLVETLANISVVLALTAFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY0</accession>
    <name>Q8TWY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Oxygen-sensitive ribonucleoside-triphosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdD</name>
        <name evidence="1" type="ordered locus">MK0901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0901"/>
    <dbReference type="EnsemblBacteria" id="AAM02114">
        <property type="protein sequence ID" value="AAM02114"/>
        <property type="gene ID" value="MK0901"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0901"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.943"/>
    <dbReference type="HOGENOM" id="CLU_002707_0_2_2"/>
    <dbReference type="OMA" id="YSYDRVP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="591" mass="67966" checksum="218D11E57FF81CC4" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX48</accession>
    <name>Q8TX48_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Succinate dehydrogenase/fumarate reductase, flavoprotein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sdhA</name>
        <name evidence="11" type="ordered locus">MK0828</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0828"/>
    <dbReference type="EnsemblBacteria" id="AAM02041">
        <property type="protein sequence ID" value="AAM02041"/>
        <property type="gene ID" value="MK0828"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0828"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.870"/>
    <dbReference type="HOGENOM" id="CLU_014312_8_1_2"/>
    <dbReference type="OMA" id="PTAHHFM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="9">
        <location>
            <begin position="7"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="10">
        <location>
            <begin position="443"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM02041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="556" mass="61373" checksum="F39C33B0CFAEBA34" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX54</accession>
    <name>SECE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secE</name>
        <name type="ordered locus">MK0822</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX54"/>
    <dbReference type="SMR" id="Q8TX54"/>
    <dbReference type="STRING" id="190192.MK0822"/>
    <dbReference type="PRIDE" id="Q8TX54"/>
    <dbReference type="EnsemblBacteria" id="AAM02035">
        <property type="protein sequence ID" value="AAM02035"/>
        <property type="gene ID" value="MK0822"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0822"/>
    <dbReference type="HOGENOM" id="CLU_191921_0_1_2"/>
    <dbReference type="OMA" id="DFLHQCR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="SSF103456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000104220" description="Protein translocase subunit SecE">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <sequence length="61" mass="6895" checksum="1AB71BE16541216C" modified="2002-06-01" version="1">MSTYEELISLLRDCKRVLRAARKPTWDEYIESAKIAGLGILIVGGVGFLIRVIVQLIELYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX64</accession>
    <name>Q8TX64_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0812"/>
    <dbReference type="EnsemblBacteria" id="AAM02025">
        <property type="protein sequence ID" value="AAM02025"/>
        <property type="gene ID" value="MK0812"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0812"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.854"/>
    <dbReference type="HOGENOM" id="CLU_071520_1_0_2"/>
    <dbReference type="OMA" id="FLLKAKC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="SSF111321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARMT1-like_dom" evidence="1">
        <location>
            <begin position="4"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33847" checksum="57032F68E69FF9B2" modified="2002-06-01" version="1">MRAVPRCVPCVLEIAVNTVERAVPEDRQKEAILAATERVSELYRMEPTPQPPRLGTEAQRTVMKYSEDPDPYREEKRRANERAAAVARELESDLENIEDPEELLKRAAAAAIVGNTIDFAVAGHEFDLDELREEISSAEFAVFDLKPEDLRGARVLYLCDNAGEIALDKLLIEVLVEELECDVVVAVRGGPIVNDATREDAEQVGITEICDVIDTGAEMLGLLATEVSEEFAEELSSADVVISKGQGNFESIPEEPFPDVPVYFLLRAKCEPVAEELGVEVGSNVALRWEPEDENVRRWREIVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX83</accession>
    <name>PUR7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="1">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purC</name>
        <name type="ordered locus">MK0792</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX83"/>
    <dbReference type="SMR" id="Q8TX83"/>
    <dbReference type="STRING" id="190192.MK0792"/>
    <dbReference type="EnsemblBacteria" id="AAM02006">
        <property type="protein sequence ID" value="AAM02006"/>
        <property type="gene ID" value="MK0792"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0792"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.833"/>
    <dbReference type="HOGENOM" id="CLU_061495_2_0_2"/>
    <dbReference type="OMA" id="EFCYKND"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100910" description="Phosphoribosylaminoimidazole-succinocarboxamide synthase">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <sequence length="247" mass="28405" checksum="0B303A4B25692EDF" modified="2002-06-01" version="1">MERGRLVYEGKAKSLYEHPEDENLLVMEFRDDITAFNMEKMDTVEGKGVYNCLISARLFEVLEDAGIPTHYVELADERRMVVERLDMFNLEVICRNMATGSLVERLPFEEGEKLDPPIVEFDYKSDEYGDPMVNMDHIRALGLATEEEVERMRELTLQVNEVLSEFLKDCDIILVDFKLEFGVNPDGEVVVGDEISPDTCRFWDAETEESLDKDIFRKDEGDVLAGYREAAERILRGDEEKLAMLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX95</accession>
    <name>Q8TX95_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01994">
        <property type="protein sequence ID" value="AAM01994"/>
        <property type="gene ID" value="MK0780"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0780"/>
    <dbReference type="HOGENOM" id="CLU_691894_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="28"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="246"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="398" mass="43401" checksum="15834B76A4394775" modified="2002-06-01" version="1">MVDLDCCVVCGDSPESIVDEVVERLGDRADFGIVFFHDIDPEDVVSELSYGVERFVGCVAGEGHYPGSVKGHKISLLALKTEWMAKFGTGGSARQDPGEASRGALEEAFEDLDFDPYDVSYSALNLKDPKRIVMYRPVIGITFIEGATFHNLGGTGLEVLSGFRFGSGPAAESIRTFGALSSDPELESAPVVCDKGVFESGAVYATISTFLKVSWSFASSLKPVTKLGTVTKSRENVIVEIDGRPAGEVYIEKLEEVTDYVKACPNEYVYKELPPLGVVRVFPSVGHRIIPRTPLEVTDDYIRTAGYVVEGEQLLLLGFDDRPNAPVRAYQGSLSSGEEPLASILVTFAGRKPLKEEIPDKHCVGMYSFGEIIPITGYNEFHNHVSACLTIFREPVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA8</accession>
    <name>Q8TXA8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphate starvation-inducible protein PhoH, predicted ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">phoH</name>
        <name evidence="4" type="ordered locus">MK0766</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0766"/>
    <dbReference type="EnsemblBacteria" id="AAM01980">
        <property type="protein sequence ID" value="AAM01980"/>
        <property type="gene ID" value="MK0766"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0766"/>
    <dbReference type="HOGENOM" id="CLU_730800_0_0_2"/>
    <dbReference type="OMA" id="YLRGRTF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="20"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="371" mass="41526" checksum="BA7FE85E379CB0BC" modified="2002-06-01" version="1">MKELMGVEPQTEGQERLVEALLNEENEIVAVFGPTGTGKTLFCAAYGVQAVMEGEYDRFIVTRPLVDVATKQEMSSADLPEKFEEMVVTPVMDVLSRFTGRDELQQLVDEGKIMIVDTHFVRGRTFDDAVILLDEVQNMLPENAGEVLARMGHNSRLLITGDPVLQKDVDIDRCGATVMREVLAEEPKAEVVDLGTRDIVRPGAERGVHLQLEIRVRNREMNDVEREIMDVVQVEAPDADVLTVLYVEPIAEDLEIKTENVPQAVIVTKEGHVGRVVGTGGERIERIEAETNMRIVVTHLTLDFVDFIADIHPVPWIGERIVDVDIAGPNLKVWIDQKDFGPFMGQRGRYARYVEEVLQELLGMGLEVEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB3</accession>
    <name>Q8TXB3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-ribbon containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01975">
        <property type="protein sequence ID" value="AAM01975"/>
        <property type="gene ID" value="MK0761"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0761"/>
    <dbReference type="HOGENOM" id="CLU_202284_0_0_2"/>
    <dbReference type="OMA" id="CRMEYER"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019216">
        <property type="entry name" value="DUF2116_Zn-ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09889">
        <property type="entry name" value="DUF2116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004990">
        <property type="entry name" value="UCP004990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="63" mass="7071" checksum="FE7F4CE5D0A7F8E4" modified="2002-06-01" version="1">MTERVPPHSHCIVCGAAIPEGERFCSEKCRMEYERRRKKAATLQWMLAGALIALGVVLMLRGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB5</accession>
    <name>RF1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prf1</name>
        <name type="synonym">erf1</name>
        <name type="ordered locus">MK0759</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXB5"/>
    <dbReference type="SMR" id="Q8TXB5"/>
    <dbReference type="STRING" id="190192.MK0759"/>
    <dbReference type="EnsemblBacteria" id="AAM01973">
        <property type="protein sequence ID" value="AAM01973"/>
        <property type="gene ID" value="MK0759"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0759"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.799"/>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="OMA" id="GQEMEVV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PTHR10113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="SSF55481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03676">
        <property type="entry name" value="aRF1/eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143175" description="Peptide chain release factor subunit 1">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00424"/>
        </source>
    </evidence>
    <sequence length="409" mass="46485" checksum="738B2908F1F6140D" modified="2002-06-01" version="1">MAESSTVERYRFRKMIERLENLRGQGTELITIYIPPENRLSDVIAQMREEYSQASNIKSKRTRKNVQSAIEVVMQRLKMVGETPENGLVVLVGTVQDGTKEKMVAELIEPPEPVDRFIYRCDSKFYLEPLKEYLEEKDVYGILVMDRREATIGLVKGKRIEPVKRLTSDVPGKHKAGGQSQRRFDRLIEHAAHEFYQKVGEAAREAFEDVKDLKGIIVGGPGPTKEEFLDGDYLPKDLKEKVLTVVDVGNTDESGLREALNKAEEALKEAELVREKRLVRKFMEEAVNGELAAYGEEVDELLKMGAVEVLLVSEDLEGYKVILRCPECGYENIVTVKEKDEAKKYVEECPECGEAELNVEEIKDIVDYYVELAEQMGSNVEIISTETEEGAQFYNAFRGLGALLRFRPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE3</accession>
    <name>Q8TXE3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="6">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">sucC</name>
        <name evidence="9" type="ordered locus">MK0731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0731"/>
    <dbReference type="EnsemblBacteria" id="AAM01945">
        <property type="protein sequence ID" value="AAM01945"/>
        <property type="gene ID" value="MK0731"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0731"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.771"/>
    <dbReference type="HOGENOM" id="CLU_037430_4_0_2"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="ATP-grasp" evidence="8">
        <location>
            <begin position="10"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding; shared with subunit alpha" evidence="6">
        <location>
            <begin position="297"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="359" mass="38976" checksum="9BAD510903F213C7" modified="2002-06-01" version="1">MIRLLEYQAKHLLKEAGVPIPEGDVARTSADAARIAAELGGPVAVKAQVPVGARGKAGGILFADDPEGARKAARKLLGSRIRGETVRKVLVEEKLDIAEEWYVSITLDRAKRRPVLLVSREGGVDIEEVPDEKIARRYLDPILGLRPFEAREAILEAGIPKEHLRDVEEVITSMYEVFESYDAHLVEINPLVLTENGEVVAADAVVNLDEDAANIRHPEFAEETRDFPFVELDGDIGIIANGAGLTMATIDLVKDLGGKPANFLDVGGGAYPTLIRRAILTVAELDVKVILLNIFGGITRCDEVAEGIVQALDDVDVPLVVRLVGTNEEEGHRILREHGVDVYTELKEAVERAVELAGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF7</accession>
    <name>ACDA1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit alpha 1</fullName>
            <shortName evidence="1">ACDS complex subunit alpha 1</shortName>
            <ecNumber evidence="1">1.2.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex carbon monoxide dehydrogenase subunit alpha 1</fullName>
            <shortName evidence="1">ACDS CODH subunit alpha 1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhA1</name>
        <name type="ordered locus">MK0717</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 7 [4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </cofactor>
        <text evidence="1">Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF7"/>
    <dbReference type="SMR" id="Q8TXF7"/>
    <dbReference type="STRING" id="190192.MK0717"/>
    <dbReference type="EnsemblBacteria" id="AAM01931">
        <property type="protein sequence ID" value="AAM01931"/>
        <property type="gene ID" value="MK0717"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0717"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.758"/>
    <dbReference type="HOGENOM" id="CLU_361186_0_0_2"/>
    <dbReference type="OMA" id="LCIGHNV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01916">
        <property type="entry name" value="ACS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01137">
        <property type="entry name" value="CdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004460">
        <property type="entry name" value="CDHA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016101">
        <property type="entry name" value="CO_DH_a-bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00314">
        <property type="entry name" value="cdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000155078" description="Acetyl-CoA decarbonylase/synthase complex subunit alpha 1">
        <location>
            <begin position="1"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="381"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="1">
        <location>
            <begin position="418"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>CO</name>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="525"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="560"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01137"/>
        </source>
    </evidence>
    <sequence length="760" mass="84637" checksum="E82B0EF7D4163CAA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL4</accession>
    <name>Q8TXL4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyltransferase</fullName>
            <ecNumber evidence="8">6.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hypF</name>
        <name evidence="12" type="ordered locus">MK0648</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0648"/>
    <dbReference type="EnsemblBacteria" id="AAM01863">
        <property type="protein sequence ID" value="AAM01863"/>
        <property type="gene ID" value="MK0648"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0648"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.687"/>
    <dbReference type="HOGENOM" id="CLU_009164_0_0_2"/>
    <dbReference type="OMA" id="VQHHYAH"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046944">
        <property type="term" value="P:protein carbamoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="10">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="11">
        <location>
            <begin position="201"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="762" mass="85051" checksum="667367382DFED439" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL8</accession>
    <name>Q8TXL8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase of the PP-loop superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0644"/>
    <dbReference type="EnsemblBacteria" id="AAM01859">
        <property type="protein sequence ID" value="AAM01859"/>
        <property type="gene ID" value="MK0644"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0644"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.683"/>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="OMA" id="NYALYWA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="PTHR12196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthami_syn_2" evidence="1">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="225" mass="25366" checksum="52A7950BE2268AE6" modified="2002-06-01" version="1">MRAVALLSGGKDSTLAAHLAVEEGYELTHGLTVVPSDPESMMFHVPNADLGALVAQVMGLEPVRIRSGRDDEADIEEIARVLEGLDVDALVSGAIASRYQKERLDRLCEELGIEHVHPLWGMDPFEELELLVERGFEVMIIGVSAAGMDESWLGRRIDEDFIEDIRRLYEKYRIHPAGEGGEYETLVLDAPLFERRIVLERVEKRWDGFSGELIVEEARLMPKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU1</accession>
    <name>Q8TXU1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0569"/>
    <dbReference type="EnsemblBacteria" id="AAM01784">
        <property type="protein sequence ID" value="AAM01784"/>
        <property type="gene ID" value="MK0569"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0569"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.604"/>
    <dbReference type="HOGENOM" id="CLU_059741_0_0_2"/>
    <dbReference type="OMA" id="LTVGTDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035402">
        <property type="entry name" value="DUF1611-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035086">
        <property type="entry name" value="DUF1611_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011669">
        <property type="entry name" value="UCP026760"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40690">
        <property type="entry name" value="PTHR40690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07755">
        <property type="entry name" value="DUF1611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17396">
        <property type="entry name" value="DUF1611_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026760">
        <property type="entry name" value="UCP026760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1611_N" evidence="2">
        <location>
            <begin position="45"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1611" evidence="1">
        <location>
            <begin position="147"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="345" mass="37419" checksum="8AA050097C280336" modified="2002-06-01" version="1">MELPHKPGTPAIILCHGAFDEPHGKTAHGLLRFGRIYDIVAVIDRELAGKTAQDVDPDFPPVPILRSVSEALEELDPEVLLIGAAPPGGKLTPEWKEEIIEAVQAGLDVVSGLHEFLSKDPDISEAAEESGSRLIDVRKPRKELFRVADGSARDVDATVVLTAGTDCAVGKMSAAIELVERLREEGVEAAFLATGQTSIMIGAEDGVVVDRMPGDFMAGAIEELVVRLAEDHEIVVVEGQGALSHPAYSGVTLAILHGAWPDAVVLVHDPVREVRDGFPRFRVPDPRTEVTLIESLSAAEIVSVVLRTWDERLAEKLSSEGYLVERLGDLRRTVDRVLEIHRMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU2</accession>
    <name>Q8TXU2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted enzyme related to O-succinylbenzoate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0568"/>
    <dbReference type="EnsemblBacteria" id="AAM01783">
        <property type="protein sequence ID" value="AAM01783"/>
        <property type="gene ID" value="MK0568"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0568"/>
    <dbReference type="HOGENOM" id="CLU_923200_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="1">
        <location>
            <begin position="90"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="301" mass="33146" checksum="99EEC8889F02146C" modified="2002-06-01" version="1">MGSLETEPVSLSLKRPFRISGREVRRVRGYWCTCVIEGNSGRGFGLRRGIAEEMATLDAVARRYDEPLCRVWGREPASIESFATVDLVGAEQAEKLARRYYLRGYRKLKVKVGGDDLKRDLERVEACVKSAEFDALILDGNEGLTVEEVFRLLDFLDFDGDVYLEHPTPPDSLEEVCESCEAPVIVDVMDLGARSIDDVLDVAEPCDIVNIKTQEVGFVGGLRLARDAREEGFKVMVGCAVESFSSISAAAHLAAAVEADLCDLDGHLFLDEDVVSNPGYAPVMVTEGPGWEVRVERPKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU8</accession>
    <name>Q8TXU8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pitA</name>
        <name evidence="8" type="ordered locus">MK0561</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0561"/>
    <dbReference type="EnsemblBacteria" id="AAM01776">
        <property type="protein sequence ID" value="AAM01776"/>
        <property type="gene ID" value="MK0561"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0561"/>
    <dbReference type="HOGENOM" id="CLU_015355_0_1_2"/>
    <dbReference type="OMA" id="GYDITRT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="323" mass="33319" checksum="5B85477E20EE7C45" modified="2002-06-01" version="1">MNVLEVLVVVVPSLYMGWTIGADDAGAAMGSAVGAGVRTMRQAVTLIAVFTTMGAVLEGHKVVKTLGKGVVQAHLDPIAAGATVLVAAAWYHLAVMLGVPVSTTQATVGSIAGVGCALGLPVNWSKFGQIAVGWFLSPILAMIPAYFVSRRLRAVLEKGKWPLAEIERKIGWLLTISGCYVAYTIGANNAANAVAPIVMTGLLDVRSAAIVGGIMIAVGALTAGSKVIETVGRKITRLDPVTAFSAELSAAIVTHGASELGIPISINETTAGAVIGVGLSRGELNTHTLKKIFTTWIASPVGSFVMSYALMRLYLSVRGAAVP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV5</accession>
    <name>MOAC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="1">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaC</name>
        <name type="ordered locus">MK0554</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaC family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXV5"/>
    <dbReference type="SMR" id="Q8TXV5"/>
    <dbReference type="STRING" id="190192.MK0554"/>
    <dbReference type="EnsemblBacteria" id="AAM01769">
        <property type="protein sequence ID" value="AAM01769"/>
        <property type="gene ID" value="MK0554"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0554"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.589"/>
    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
    <dbReference type="OMA" id="IWDMVKS"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01419">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000097855" description="Probable cyclic pyranopterin monophosphate synthase">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <sequence length="156" mass="17004" checksum="936C82A4FEE5D965" modified="2002-06-01" version="1">MTAGGRRMSMVDVTGKKEEIRIAEASGFLRLTEDGVNAVKSGESHPQGKGDPIEVAKVAAILAVKKTPELVPHCHPIKITGVDVDVEVLEDGVKMSVRVKSEGKTGVEMDALTGLVVGLVTLWDMVKYAEKDEEGQYPHTRIENVRVVEKIIKEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV6</accession>
    <name>Q8TXV6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdopterin biosynthesis enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">moaB</name>
        <name evidence="4" type="ordered locus">MK0553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0553"/>
    <dbReference type="EnsemblBacteria" id="AAM01768">
        <property type="protein sequence ID" value="AAM01768"/>
        <property type="gene ID" value="MK0553"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0553"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.588"/>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="17"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="18635" checksum="D0593D6D8C921DE0" modified="2002-06-01" version="1">MPVEKHVERAPKRLAIAVITVSTSKFEEAARGEEPEDTSGDILESRFEQAGHETRYRVLIPDQREMVAGAVKWTANRVDAVVTTGGTGLTPTDVTIEAVGEIAEKQVPGFGELFRRKSEEDVGAHSILSRAEMFVVDGTPVACLPGSPNAVKLGAELLIEVLPHVVVHSRGDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV8</accession>
    <name>GUAAA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAA</name>
        <name type="synonym">guaA_2</name>
        <name type="ordered locus">MK0551</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXV8"/>
    <dbReference type="SMR" id="Q8TXV8"/>
    <dbReference type="STRING" id="190192.MK0551"/>
    <dbReference type="MEROPS" id="C26.A31"/>
    <dbReference type="EnsemblBacteria" id="AAM01766">
        <property type="protein sequence ID" value="AAM01766"/>
        <property type="gene ID" value="MK0551"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0551"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.586"/>
    <dbReference type="HOGENOM" id="CLU_014340_1_4_2"/>
    <dbReference type="OMA" id="GPDMDRI"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140222" description="GMP synthase [glutamine-hydrolyzing] subunit A">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <sequence length="187" mass="21042" checksum="A76FEDA8E3D7B304" modified="2002-06-01" version="1">MILIIDNHGQYVHLIRKNFDYMGVPAEIIPNTTDPEDVRERASGVVISGGPSRERAGNSREIIEELTGEVPILGICLGHQLMAEVFGGKVDWAAGREEYARTEVEILDHEGIFEGLPDKIVAWASHRDEVKEVPDEFVVTARSDRCEVEAMRHEELPLYGVQFHPELKFTEYGPDILKNFAKLCGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW4</accession>
    <name>Q8TXW4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetolactate synthase small subunit</fullName>
            <shortName evidence="6">AHAS</shortName>
            <shortName evidence="6">ALS</shortName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvH</name>
        <name evidence="8" type="ordered locus">MK0545</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0545"/>
    <dbReference type="EnsemblBacteria" id="AAM01760">
        <property type="protein sequence ID" value="AAM01760"/>
        <property type="gene ID" value="MK0545"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0545"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.580"/>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_2"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="18502" checksum="C3276E4135599A12" modified="2002-06-01" version="1">MLVKGGCELSKRRVLSVLVKDRPGVMQRVSGLFRRRGFNIDSIAEGPSEREGLARMTLTVKGNEQTIEQVVKQLNKLVDVIKVSELDPERTVERQLALVKIRTKDRARAAELARAAGAEVVDVGRETITVEIVGEPQDVESLLELMKDIGNIVEIARTGIVAMERET</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY19</accession>
    <name>Q8TY19_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0487"/>
    <dbReference type="EnsemblBacteria" id="AAM01702">
        <property type="protein sequence ID" value="AAM01702"/>
        <property type="gene ID" value="MK0487"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0487"/>
    <dbReference type="HOGENOM" id="CLU_112469_1_0_2"/>
    <dbReference type="OMA" id="HFWRGSH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002782">
        <property type="entry name" value="Mut7-C_RNAse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01927">
        <property type="entry name" value="Mut7-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mut7-C" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="152" mass="17764" checksum="0656C52CC8F6A38B" modified="2002-06-01" version="1">MLLRLCRWLRLLGYPTVAAHEVVDPTKVEDEDEVLVEFCREHDALLITRDRQLARRARAVLITADSVPEQIAEVLEFLGDEAFLDPDESRCPECNARVRRVTAENPGPFEVTWECPECRRKYWVGGHWRDMEETIDRVKKALRRVQRGHLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY40</accession>
    <name>Q8TY40_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaL</name>
        <name evidence="2" type="ordered locus">MK0466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0466"/>
    <dbReference type="EnsemblBacteria" id="AAM01681">
        <property type="protein sequence ID" value="AAM01681"/>
        <property type="gene ID" value="MK0466"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0466"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.496"/>
    <dbReference type="HOGENOM" id="CLU_2366257_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019211">
        <property type="entry name" value="EhaL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011305">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09877">
        <property type="entry name" value="DUF2104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004953">
        <property type="entry name" value="EhaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="114" mass="12641" checksum="8013EEB64CB8637E" modified="2002-06-01" version="1">MIGFEGRMFVEAVIAGAVAMFLVGSLVGLEYSYRMYPEIFRERRVDPISLLLAVLGWTLLAVSWMRSVPVGIWIAAFLIGYVVNMRPGYGRIETVLGIATFLAALTWLEGLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY41</accession>
    <name>Q8TY41_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ehaM</name>
        <name evidence="1" type="ordered locus">MK0465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01680">
        <property type="protein sequence ID" value="AAM01680"/>
        <property type="gene ID" value="MK0465"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0465"/>
    <dbReference type="HOGENOM" id="CLU_1773196_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036606">
        <property type="entry name" value="EhaM-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012056">
        <property type="entry name" value="NiFe-hyd_3_EhaM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09218">
        <property type="entry name" value="DUF1959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101332">
        <property type="entry name" value="SSF101332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="17106" checksum="8AA066642BA01025" modified="2002-06-01" version="1">MMRFLIRRKNITEEDLNERYAEDPNLVLRIKKWILTSYAYRRDIVHRLAELLNVDEDHVIDVLSRARSCSGLYGLHSEVEQAERLLDNVDDEVITLAVLMDVVSDGKLSEALEDELLRMFEGRKSVPIDRKELTKFFTSLRERGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY48</accession>
    <name>Q8TY48_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0458</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0458"/>
    <dbReference type="EnsemblBacteria" id="AAM01673">
        <property type="protein sequence ID" value="AAM01673"/>
        <property type="gene ID" value="MK0458"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0458"/>
    <dbReference type="HOGENOM" id="CLU_1286363_0_0_2"/>
    <dbReference type="OMA" id="AIWACGG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016486">
        <property type="entry name" value="UCP006591_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006591">
        <property type="entry name" value="UCP006591_CBS_MJ1004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="67"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="212" mass="24114" checksum="E35591C5155119DE" modified="2002-06-01" version="1">MRAEMWATKEYPRVKPEDSLEQAVRELTRYSEYTAVVVNGSDRLVGLVTSNDIARGLTAGAETVEEVCRSPESISPSDPITKAVEILTDSDLTLVPLEEDMRVVGVVTLRTVVELMSNLYDTPARDLLEKIHENVPGISWDEFIEAATMVFNRELNRDLTPEEFERKISDRTFGYVLWLMGGLENLFIYLFRLGEAVVARKVAKRRRELRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY78</accession>
    <name>Q8TY78_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01642">
        <property type="protein sequence ID" value="AAM01642"/>
        <property type="gene ID" value="MK0427"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0427"/>
    <dbReference type="HOGENOM" id="CLU_1521880_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="176" mass="19539" checksum="119D917F4376CB3F" modified="2002-06-01" version="1">MLITAPHAQGPGADVFTGEIAWKVAQATGAYALVATVSRRAKLEDGNPADYNREWARNTPFRRRIDELIKRYGVRFIIDVHGMESDPVRPDVDLGTLGGRSAKGKLVSKIVKRLEEAGFDVGFEQEFQGGDILEYHCDGERVQGVQLELSEELRELGEYRAIQAVLVVVNTVLEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY80</accession>
    <name>Q8TY80_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-dependent hydrolase of the TIM-barrel fold</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01640">
        <property type="protein sequence ID" value="AAM01640"/>
        <property type="gene ID" value="MK0425"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0425"/>
    <dbReference type="HOGENOM" id="CLU_564550_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="483" mass="55196" checksum="7E08DFC610766378" modified="2002-06-01" version="1">MTRLFFRRRSVESFAERIEVKVGVEEELFLIDRDGSLTRAADDVIVKAAELLESDSNLLEDCWRTVLGLDPEPNPAQIEYLTQPLPPDEVIEACEIGRELIKKAAEELGLQVMLESMHPFESDPLPINGTHINVMVKLKDQPYMTPKQILVVYNWLWHNLPIIIAATANTPYCCGGKNLAASCRLLKSRVLKPNYYAAIKRLEKRPYLTKTQYYGRLRYRLRLRKDTEFEERVVAHPDGRRLVDITPRGPASNVTGDENDSPTRNRVEVRVIDNQKSMKYLHDVVMLIVGLSLEALYIYEVEGKLPPNDPNHFDNRREAIEKGINATFVINGREIDAEDALLKIISRVDKFLEHLGLRFVSPLKNGKVELQERPKLNVEYVHKDVIKYIGNYAEVILGSNKTVEIKGKRYTIPKGTKVIGKLVPMASYKYRVDNKGFVKDIIKGVVTLGIKRNGVEIPLDESDRIVNVMSELEYLMRSMRGLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY87</accession>
    <name>RS27_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S27e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27e</name>
        <name type="ordered locus">MK0418</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY87"/>
    <dbReference type="SMR" id="Q8TY87"/>
    <dbReference type="STRING" id="190192.MK0418"/>
    <dbReference type="EnsemblBacteria" id="AAM01633">
        <property type="protein sequence ID" value="AAM01633"/>
        <property type="gene ID" value="MK0418"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0418"/>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="OMA" id="DCENEQV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_S27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_S27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000149073" description="30S ribosomal protein S27e">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <sequence length="73" mass="8281" checksum="FE715056D29C0E8B" modified="2002-06-01" version="1">MDEKRFLKRFTESDLVPQPRSRFLRVECVDCGNEQIIFGNASTEVKCHICGRTLAKPTGGKAKILTKIKEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA0</accession>
    <name>Q8TYA0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01620">
        <property type="protein sequence ID" value="AAM01620"/>
        <property type="gene ID" value="MK0405"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0405"/>
    <dbReference type="HOGENOM" id="CLU_2271064_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="102" mass="11836" checksum="BC6689DAC5D9F83D" modified="2002-06-01" version="1">MSGTIKLSRCGLRAPTCEPVRRSYFERLRRYRDYQVRELYESGVRIKVLARVFDLSEGEIKEILLSPPRCARCGKPLRTNVPLCSECERVLEGDDRSRTDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF1</accession>
    <name>Q8TYF1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01564">
        <property type="protein sequence ID" value="AAM01564"/>
        <property type="gene ID" value="MK0349"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0349"/>
    <dbReference type="HOGENOM" id="CLU_1145229_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="242" mass="26371" checksum="908C98ABBBD6A3C4" modified="2002-06-01" version="1">MYRTLTLLVLSTTLMAVAAAERLDPVDEDRPHILNVYIEGHEATCFGQGIDTKRLETLGLKHVPEVPTKGEFTVTVEFNTGPHPPERLAVELYFPSWLMVPPKYDLKLPEKGVEIDHPEPNSILRADLEVKVGEGPNPLSIGDEYYLVAVLRDPTKESDVLDYVVFKLRFREPRGGESTPEPTLLKVTRSALGDVSVPVAGFILGSLSALVAEIVCGWIHSGPAALWATVIGGLVGALLVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ4</accession>
    <name>Q8TYJ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted cysteine protease of the transglutaminase-like superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01520">
        <property type="protein sequence ID" value="AAM01520"/>
        <property type="gene ID" value="MK0303"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0303"/>
    <dbReference type="HOGENOM" id="CLU_887428_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="219"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="313" mass="35927" checksum="57210E56AF8970C6" modified="2002-06-01" version="1">MSMLNAEPKWFQWVEGCDWAKYLATRDTPIVDWTGELIGENWRANWVRAAKYILYCAPYKGDSVEKVRQAWYGSITLEGHVPDKGAPAKFGEVDWSDKRPDMRYIGLAFDYFDPVFDFLHVPEDLRRTMRRLAELMVGLGKVSEREYAESILKDEYMPPRSGEPSRSREYEIRNGQYWLPGEWCWITLNGTSLYGKVAKSPAQPFPNAGEAERKLMNNNPMFQAAVRLAYWVATHIPFIGWFNWFPLAGYCGHLSPMYYEGPAAPLLFPDEGWVCTDHAVLTVALYRAAGIPGYVGGELEPFGDHSWTAVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ5</accession>
    <name>Q8TYJ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA-binding protein containing PIN domain, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01519">
        <property type="protein sequence ID" value="AAM01519"/>
        <property type="gene ID" value="MK0302"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0302"/>
    <dbReference type="HOGENOM" id="CLU_1264575_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="353" mass="40412" checksum="28D0DBD97A35B4AE" modified="2002-06-01" version="1">MVPAPLALVLSTLLTVQPANSLQWKDCPWIQLGRLSCATQGLAVDYPYDLAGDHLLLVSPLWPEGDHYRVILAAYGVEELPLPLGTRVHRVFVPSEVRRVPWKLWEQIPESPRPATFGPWSEVRYSLREWGCPQGYVFDPDEWDWSELQSSLELAVLLEPERTVDGVFAVEASLPVREDRIGKERWIRLALTPGRWLVLLGWTEDFDVRTPCRTLFRGGAPYDEAEYGLDGVKLRFWVAEYLLDLGGRARIPPSEYYSSVAGLFDFLNELPGGWEFRPGLQHEHRLYYGYGVLLEVRADRSLIVEFKAPEAGKRFRSADTVDQWFLSSRYIARHSHLLEPLRKILRGSRPDGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ8</accession>
    <name>Q8TYJ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01516">
        <property type="protein sequence ID" value="AAM01516"/>
        <property type="gene ID" value="MK0299"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0299"/>
    <dbReference type="HOGENOM" id="CLU_870479_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="345" mass="37945" checksum="F5D31015BFA3D9E4" modified="2002-06-01" version="1">MLPYLVLGLVLALTPASGEPLIQGSLSKDLEFLHEYFQKRVLPKWKDDPHVIVDVVQYSESAGYVIYLPKENLFAFSRVQESVEPGRVRVVLPLTCGRPVAVEERLEGPAVIASVNQCPLTEGSSANLGLDVVVGDHVCSFGYVTNVPALGAPARCEYVLTLSAGGRVVDEEGLGSFDPVDVEYEVYSDRVVMRILGFPEGVEDLALVIDATFDLRKGRILVDRRESTDSELVSKLRELRARWGTPPGEGIEGFSGDLLRTRSGAEPLWVGFQLSHDDPLVRFVEKVIGMYSQGASEKELQKVMGEHPNVEFLFPERNGESGTSKIPFWVLPLLPVPLAGRRNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK7</accession>
    <name>Q8TYK7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse gyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rgyA</name>
        <name evidence="5" type="ordered locus">MK0289</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0289"/>
    <dbReference type="EnsemblBacteria" id="AAM01506">
        <property type="protein sequence ID" value="AAM01506"/>
        <property type="gene ID" value="MK0289"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0289"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.293"/>
    <dbReference type="HOGENOM" id="CLU_772961_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="PTHR43505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TOP1Ac" evidence="4">
        <location>
            <begin position="31"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="358" mass="41700" checksum="E0E90D3815B8B2E4" modified="2002-06-01" version="1">MNATLRIRNRPVAESTYTSLRGAKAEVVRVEREEREIHPKPPFETGTMLQAATRRLRLSSERVMQLAQDLFEGGLITYHRTDSTRVSEEGKRVARDYIRANFDPEDYNPRTWEPEAEHVEGAHECIRPTRPADAEELRTMVREGAIQTTVTLTSHHLRLYDLVFRRFVASQMKPAKVLYQEAVLEVEVKGVPVAELELSGVLEIVEPGFTKVLTEYDLPAYGIRETPELEEGDRLEIGDVEVLERHEEYPYDQSELVEDMRERGLGRPSTYAQIVEKLFRRGYVYEVPQRRWIFPTTRGEAVYEYLSTHYERFVSEETTRDLEERMDAVALGKAEYQEEMEKLYLELERVVEMPDPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL1</accession>
    <name>Q8TYL1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01502">
        <property type="protein sequence ID" value="AAM01502"/>
        <property type="gene ID" value="MK0285"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0285"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.289"/>
    <dbReference type="HOGENOM" id="CLU_078138_0_0_2"/>
    <dbReference type="OMA" id="SKRRRMY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016754">
        <property type="entry name" value="UCP019262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09894">
        <property type="entry name" value="DUF2121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019262">
        <property type="entry name" value="UCP019262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="322" mass="35900" checksum="2A1DF166797A01E3" modified="2002-06-01" version="1">MTLVLAYAGTDGALVAGDRRTLVARMDEEKMRKVEEKLYSGEIRTEEELESFLKDLDVEDGYFEFHDDRKKVWKVNDEVVAGEVGVRSAKGVRRRRVYATPGAHAIVELEGEKVLSKNFGGPALIVEGPKVVKELVIEFVNSELGGKPDLESLRNALDDLFEYVSSGTILVSSEYDAYEVKGKADPLARARLQKAIDEDIERLREHRRRLAEEMLKHIREGYDILKEGVVGEVVEVGTEEEGKGVEDVPPERRIVVRLAEDVDARHMGDVVAGPGEEIVMAVEGNPEEVEPGDRVVVKDGVMKIDGKDLPVITGYSICRTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ6</accession>
    <name>Q8TYQ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01453">
        <property type="protein sequence ID" value="AAM01453"/>
        <property type="gene ID" value="MK0236"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0236"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.237"/>
    <dbReference type="HOGENOM" id="CLU_434531_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="629" mass="68522" checksum="DDC6081B99034908" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU4</accession>
    <name>Q8TYU4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-associated phospholipid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pgpB</name>
        <name evidence="3" type="ordered locus">MK0197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01414">
        <property type="protein sequence ID" value="AAM01414"/>
        <property type="gene ID" value="MK0197"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0197"/>
    <dbReference type="HOGENOM" id="CLU_1709133_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="50"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="153" mass="17239" checksum="8C24717F82ADBB5F" modified="2002-06-01" version="1">MAEIVVRFDHRVIEKIPRRPHPVFELIGFIFSGWVLFPSSLILMLFDRPLGFQVLEALVLSMVSSEILKVVVGRPRPQREGRTPWGYSFPSTHTARVASLIPVFWKLSSNLGIVVVVVAIIVAISRVLSRAHYPSDVVAGFLLGYVVGWFVIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX2</accession>
    <name>Q8TYX2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted phosphatase of the PHP family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01386">
        <property type="protein sequence ID" value="AAM01386"/>
        <property type="gene ID" value="MK0169"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0169"/>
    <dbReference type="HOGENOM" id="CLU_061999_1_0_2"/>
    <dbReference type="OMA" id="MMDLHTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="2">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="236" mass="25846" checksum="A95F2A118D554C06" modified="2002-06-01" version="1">MRLRADLHTHTVYSDGHGTPLENVLAAEERGLETVALTDHGPASPDGLTDRSFRRLVAEAREAEKLCSVRVYVGVEANIVSISGEIDATPAMLSESDIVLAAIHNPRLILANPGSAEELRRAIVHATIRCIESGEVHIIAHPVWILEQLRCYITAQEAEEIARVAADHNVGLELNARHLPRDFTLYQVAIRVGAPITFGSDAHAPEEVGRFKPLQKLARRLGIEPQDVHPEELGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ7</accession>
    <name>Q8TYZ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0144</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01361">
        <property type="protein sequence ID" value="AAM01361"/>
        <property type="gene ID" value="MK0144"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0144"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.143"/>
    <dbReference type="HOGENOM" id="CLU_2802251_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="3">
        <location>
            <begin position="47"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="263" mass="29427" checksum="B0A083A0D17B29C0" modified="2002-06-01" version="1">MRSRRGFRALPPGPRPPRTPDDPARVIEDKIKECVRRSGHPRPGSRILVAMSGGKDSFAVAFGLKRLRGGYGWEVEGAHVAPTVDGKKISAWDVVEEQAKILEIHAYLLEPESDVLELKEEMNARKVCYACRTLRRIELGQLAEEKGFDYIALGHTLDDAAATVILSLLTGAERLKLLWYTGTWRGGPRLIRPLVRCPEAVTKALAEELKVDTVMTEDVCPYAGGLRDEVEEFLDRLEREWIPHVKGNVVGTALRTLRGRQCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ8</accession>
    <name>Q8TYZ8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0143</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0143"/>
    <dbReference type="EnsemblBacteria" id="AAM01360">
        <property type="protein sequence ID" value="AAM01360"/>
        <property type="gene ID" value="MK0143"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0143"/>
    <dbReference type="HOGENOM" id="CLU_045498_5_4_2"/>
    <dbReference type="OMA" id="LCITRAI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="25674" checksum="F82AF8B979129DFF" modified="2002-06-01" version="1">MIPDPSMMLYLGIGGLVGLVSGMFGVGGGFLLVPLLNSTGMPMHLALGTTLLAISLGGFTGAYRHLQEGNVHVDAAPIFGLSAIVGAQVGSYLACLTPEHVLKVALGVACSAMALRMAFDGETEEGNEIRDNIAVASLTGFGVGAFSGFTGSGGGVLFVPVMASVLNFPTMLAIGTSSVIVPVSALAGAAQYWMEGYVNFWAALAVVTGMLISSYVGAELSNKIGGERVKRAFSVVLALVGAKMVLSGLRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ21</accession>
    <name>Q8TZ21_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized archaeal coiled-coil domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01337">
        <property type="protein sequence ID" value="AAM01337"/>
        <property type="gene ID" value="MK0120"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0120"/>
    <dbReference type="HOGENOM" id="CLU_063607_0_0_2"/>
    <dbReference type="OMA" id="SEFRQEQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="151"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="234"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="316" mass="38709" checksum="931BB1D3A4DFD523" modified="2002-06-01" version="1">MMLSEDKLQKIKELENQLVKTREELDDLEEKRQEIQRKIDQLRSQIHEIRERAEKYRAKRDELNERVRELRERADEHRRRRDELNEEVQQYKAKRDELNERARELAQKAREHVETAKKLRSKVGRPIREIRAEIRRLEREIETNPLSPRREEQIAQRLEELREQLRAWEKANEHSKKADELFSQADELREKASEYHEKVVKTAEEADKYHQKMIECYERADKLREKADGYHRKYVEELAKVKPLEDELRELQSELRDLEQKIMEKRAEERALEVRIKDLEKQAVDEERLKEIKERLERGEKVSWEELQLLQEYGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ28</accession>
    <name>Q8TZ28_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbonic anhydrase/acetyltransferase, isoleucine patch superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaY</name>
        <name evidence="1" type="ordered locus">MK0112</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TZ28"/>
    <dbReference type="STRING" id="190192.MK0112"/>
    <dbReference type="EnsemblBacteria" id="AAM01329">
        <property type="protein sequence ID" value="AAM01329"/>
        <property type="gene ID" value="MK0112"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0112"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.111"/>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="165" mass="17931" checksum="98692ED9E4405E98" modified="2002-06-01" version="1">MRRLKIEGRAYIHPTATVLGEVELGQDASLWPGAVVRGDLEPVRIGRESNIQDNAVVHVSKGYPVEIGDRVSVGHGAVVHGATIEEDCLIGMNATVMNGAVIRRGSIIGAGAVVTEGTEVGPYEIWVGVPAKRIGTTDEERVDEIRENARRYLKLAREELPEWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ48</accession>
    <name>Q8TZ48_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted ABC-class ATPase, RNase L inhibitor homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0091</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0091"/>
    <dbReference type="EnsemblBacteria" id="AAM01308">
        <property type="protein sequence ID" value="AAM01308"/>
        <property type="gene ID" value="MK0091"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0091"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.91"/>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="OMA" id="ISYKPQY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="PTHR19248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="80"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="349"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="565"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="569"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="381"/>
            <end position="388"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="599" mass="66848" checksum="14CA95E076F04F4F" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ86</accession>
    <name>Q8TZ86_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">amtB</name>
        <name evidence="10" type="ordered locus">MK0053</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0053"/>
    <dbReference type="EnsemblBacteria" id="AAM01270">
        <property type="protein sequence ID" value="AAM01270"/>
        <property type="gene ID" value="MK0053"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0053"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.54"/>
    <dbReference type="HOGENOM" id="CLU_1032941_0_0_2"/>
    <dbReference type="OMA" id="IWLSIMS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="27"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="431" mass="45245" checksum="E7CAA6891AEF12BB" modified="2002-06-01" version="1">MAAESIAKAVHDVLATWGAQGPGAIFFLVWAAVLVVGMQLGFMFLEAGQVRTKNVVSVLMKNLLDLSLGGIVFIFFGFALAFPDYAQSIGKWIATMFTANPWSADPMKAADPYNLAYCFFQFAFCATAATIVSGAVAERINFKACLVMTVLITGLLYPIFVLWTWGGGWMGGDTGLFAKVFGQPYHDFAGSTVVHAIGGFLAMAAAYLLGPRIGRFKNGKPVPIPGHNIPQAFLGALFLAITWYGFNVGSSAVLYDPTNESWISSLVATLAMCGGAAAAAFTTRFDPLWAANGLIAGLVAICAGCDIMSPFGALLTGIVAGLIIKPAFRLLEKLEIDDVVAACPVHGFAGVWGAIAAGIFGAEALGGAGGVSLAAQIVGALVCIAWALGSGFATFYVIDKVIGLRATEEEEKGLDETEFGVSAYPYMETRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ89</accession>
    <name>Q8TZ89_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">taw3</name>
        <name evidence="7" type="ordered locus">MK0048</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0048"/>
    <dbReference type="EnsemblBacteria" id="AAM01265">
        <property type="protein sequence ID" value="AAM01265"/>
        <property type="gene ID" value="MK0048"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0048"/>
    <dbReference type="HOGENOM" id="CLU_047426_2_0_2"/>
    <dbReference type="OMA" id="GKWHHYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSF111278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TYW3" evidence="6">
        <location>
            <begin position="7"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="193" mass="21635" checksum="24ADE40FBB695DF3" modified="2002-06-01" version="1">MHWEGEKRRRLKELERAIERGEVDEAAIPVLETLNSFEEYCTTSSCSGRVVVLHEPEVGDKIGSEFVAKWHEPPEPEEVREAVLKAPEEGITWVKAQPPLFHVMCRDLEAAVRLRNIASEAGFKASSIRSVKSSKVIVEILGGERMDVPAKVNGKLTLREKAWDSVVALCNDILRSGHERLSRLVEALKGLSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA7</accession>
    <name>RL30_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30</name>
        <name type="ordered locus">MK0028</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZA7"/>
    <dbReference type="SMR" id="Q8TZA7"/>
    <dbReference type="STRING" id="190192.MK0028"/>
    <dbReference type="EnsemblBacteria" id="AAM01245">
        <property type="protein sequence ID" value="AAM01245"/>
        <property type="gene ID" value="MK0028"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0028"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.28"/>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="OMA" id="KDYITWG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_L30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_L30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_L7/L30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_L7_euk_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="PTHR11524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000273904" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <sequence length="149" mass="17195" checksum="73C28834C10B0ADE" modified="2002-06-01" version="1">MRGPVGVRRDIEDTMRMLKLLRRNWCVLIDDRPSYLGMLQKIKDYVTWGEVEPDTVAALLKKRGELEGGRPVTDEYVSEHTEYDSVEEFARAYCEFEAELDDIPKLKPFFRLHPPRGGYERGGIKKPYTLGGALGYRGKAINDLLERMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB5</accession>
    <name>Q8TZB5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01237">
        <property type="protein sequence ID" value="AAM01237"/>
        <property type="gene ID" value="MK0020"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0020"/>
    <dbReference type="HOGENOM" id="CLU_144581_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020380">
        <property type="entry name" value="Uncharacterised_MJ1658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17393">
        <property type="entry name" value="DUF5402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="119" mass="13551" checksum="DEE15A2284ABF71B" modified="2002-06-01" version="1">MPKLNQRGLVELRRELERRLVELLGKPVHVMQLTIFALPCGCVGASLEVRNIVREDAEVFRDHLRDLLREMVKWTLGKEPDLYYARLTPSGYDVVSLTGRVACDECEKNFKGAADVLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-05-25" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVG9</accession>
    <name>F1SVG9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Activator of 2-hydroxyglutaryl-CoA dehydratase, contains a HSP70-class ATPase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01477">
        <property type="protein sequence ID" value="AAM01477"/>
        <property type="gene ID" value="MK0260"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0260"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.263"/>
    <dbReference type="HOGENOM" id="CLU_066597_0_0_2"/>
    <dbReference type="OMA" id="GKGANYF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BcrAD_BadFG" evidence="3">
        <location>
            <begin position="4"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="251" mass="26691" checksum="D518C386A2060F65" modified="2011-05-03" version="1">MRVLGVDAGSSHLKCAIVEDGSLEDHTVVESTGPVKKVLRRALDELGAGIDEFDVTAVTGYGREALSDEFDETVPELPAVALGASQLVEGARTVIDVGGQDTKVMKVEDGKVVDFQVNDKCAAGTGRFVENVCRRLGIEMSEVDEHASGADDPVKINSMCAVFAETEVISLVNRGIDVERILLGVLDSVAERVATMIDKVSPEPEVVLVGGMARCRVFAELLSDRLEMGINVPNEAHVAGAFGAALWVLEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q49610</accession>
    <name>FTR_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formylmethanofuran--tetrahydromethanopterin formyltransferase</fullName>
            <shortName evidence="1 6">Ftr</shortName>
            <ecNumber evidence="1 3">2.3.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">H4MPT formyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 6" type="primary">ftr</name>
        <name type="ordered locus">MK0116</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="Eur. J. Biochem." volume="230" first="906" last="913">
            <title>Formylmethanofuran:tetrahydromethanopterin formyltransferase (Ftr) from the hyperthermophilic Methanopyrus kandleri. Cloning, sequencing and functional expression of the ftr gene and one-step purification of the enzyme overproduced in Escherichia coli.</title>
            <authorList>
                <person name="Shima S."/>
                <person name="Weiss D.S."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="7601152"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1995.tb20635.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="210" first="971" last="981">
            <title>Salt dependence, kinetic properties and catalytic mechanism of N-formylmethanofuran:tetrahydromethanopterin formyltransferase from the extreme thermophile Methanopyrus kandleri.</title>
            <authorList>
                <person name="Breitung J."/>
                <person name="Borner G."/>
                <person name="Scholz S."/>
                <person name="Linder D."/>
                <person name="Stetter K.O."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="1483480"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb17502.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-49</scope>
        <scope>FUNCTION</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Eur. J. Biochem." volume="267" first="6619" last="6623">
            <title>A mutation affecting the association equilibrium of formyltransferase from the hyperthermophilic Methanopyrus kandleri and its influence on the enzyme's activity and thermostability.</title>
            <authorList>
                <person name="Shima S."/>
                <person name="Thauer R.K."/>
                <person name="Ermler U."/>
                <person name="Durchschlag H."/>
                <person name="Tziatzios C."/>
                <person name="Schubert D."/>
            </authorList>
            <dbReference type="PubMed" id="11054114"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.2000.01756.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>MUTAGENESIS OF ARG-261</scope>
    </reference>
    <reference evidence="9" key="5">
        <citation type="journal article" date="1997" name="Structure" volume="5" first="635" last="646">
            <title>Formylmethanofuran: tetrahydromethanopterin formyltransferase from Methanopyrus kandleri -- new insights into salt-dependence and thermostability.</title>
            <authorList>
                <person name="Ermler U."/>
                <person name="Merckel M."/>
                <person name="Thauer R."/>
                <person name="Shima S."/>
            </authorList>
            <dbReference type="PubMed" id="9195883"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(97)00219-0"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4">Catalyzes the reversible transfer of a formyl group from formylmethanofuran (formyl-MFR) to tetrahydromethanopterin (H(4)MPT) to produce 5-formyl tetrahydromethanopterin (5-formyl-H(4)MPT) and methanofuran (MFR) (By similarity) (PubMed:11054114, PubMed:1483480, PubMed:7601152). Acts via a ternary-complex mechanism. Uses N-furfurylformamide much less efficiently, does not use N-methylformamide or formamide (PubMed:1483480, PubMed:11054114, PubMed:7601152). Protein overexpressed in E.coli has very similar properties to enzyme purified from M.kandleri (PubMed:7601152).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3 4">
            <text>5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:18061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="2.3.1.101"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Requires high salt concentrations for activity and thermostability; 1.5-1.8 M KH(2)PO(4) stimulates activity while stabilizing the enzyme.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">50 uM for N-formylmethanofuran</KM>
            <KM evidence="3">100 uM for 5,6,7,8-tetrahydromethanopterin</KM>
            <KM evidence="3">20 mM for N-furfurylformamide</KM>
            <Vmax evidence="3">2700.0 umol/min/mg enzyme reaction of N-formylmethanofuran and 5,6,7,8-tetrahydromethanopterin</Vmax>
            <Vmax evidence="3">68.0 umol/min/mg enzyme reaction of N-furfurylformamide</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 6.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homotetramer composed of two dimers (PubMed:11054114, PubMed:9195883). Dimerization is sufficient for enzyme activity, but tetramerization is required for high thermostability (PubMed:11054114).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.101" evidence="1 3"/>
    <dbReference type="EMBL" id="X85115">
        <property type="protein sequence ID" value="CAA59435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S65950">
        <property type="entry name" value="S57647"/>
    </dbReference>
    <dbReference type="PDB" id="1FTR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C/D=1-296"/>
    </dbReference>
    <dbReference type="PDB" id="2FHJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-296"/>
    </dbReference>
    <dbReference type="PDB" id="2FHK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-296"/>
    </dbReference>
    <dbReference type="PDBsum" id="1FTR"/>
    <dbReference type="PDBsum" id="2FHJ"/>
    <dbReference type="PDBsum" id="2FHK"/>
    <dbReference type="AlphaFoldDB" id="Q49610"/>
    <dbReference type="SMR" id="Q49610"/>
    <dbReference type="STRING" id="190192.MK0116"/>
    <dbReference type="EnsemblBacteria" id="AAM01333">
        <property type="protein sequence ID" value="AAM01333"/>
        <property type="gene ID" value="MK0116"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0116"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.115"/>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="OMA" id="VIMCPAE"/>
    <dbReference type="BRENDA" id="2.3.1.101">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00693"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q49610"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00579">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03119">
        <property type="entry name" value="one_C_fhcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138120" description="Formylmethanofuran--tetrahydromethanopterin formyltransferase">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Weakens dimer-dimer association. Thermolabile." evidence="2">
        <original>R</original>
        <variation>E</variation>
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="7" ref="3">
        <original>H</original>
        <variation>D</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="7" ref="3">
        <original>W</original>
        <variation>K</variation>
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="7" ref="3">
        <original>E</original>
        <variation>K</variation>
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="6"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="12"/>
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        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="47"/>
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        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="53"/>
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        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
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    <feature type="strand" evidence="10">
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            <begin position="72"/>
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        </location>
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    <feature type="helix" evidence="10">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="97"/>
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        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="104"/>
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        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="10">
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    </feature>
    <feature type="helix" evidence="10">
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    <feature type="strand" evidence="10">
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    <feature type="strand" evidence="10">
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    <feature type="strand" evidence="10">
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    <feature type="strand" evidence="10">
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        <location>
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    <feature type="helix" evidence="10">
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11054114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="1483480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="7601152"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="9195883"/>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="7601152"/>
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    </evidence>
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        <source>
            <dbReference type="PubMed" id="1483480"/>
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            <dbReference type="PDB" id="1FTR"/>
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    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="1FTR"/>
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    <sequence length="296" mass="31662" checksum="DDE02D3E7D98FC86" modified="1996-11-01" version="1">MEINGVEIEDTFAEAFEAKMARVLITAASHKWAMIAVKEATGFGTSVIMCPAEAGIDCGYVPPEETPDGRPGVTIMIGHNDEDELKEQLLDRIGQCVMTAPTASAFDAMPEAEKEDEDRVGYKLSFFGDGYQEEDELDGRKVWKIPVVEGEFIVEDSFGITTGVAGGNFYIMAESQPAGLQAAEAAVDAIKGVEGAYAPFPGGIVASASKVGSKQYDFLPASTNDAYCPTVEDNELPEGVKCVYEIVINGLNEEAVKEAMRVGIEAACQQPGVVKISAGNFGGKLGQYEIHLHDLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS5</accession>
    <name>PSTK_METKA</name>
    <protein>
        <recommendedName>
            <fullName>L-seryl-tRNA(Sec) kinase</fullName>
            <ecNumber>2.7.1.164</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>O-phosphoseryl-tRNA(Sec) kinase</fullName>
            <shortName>PSTK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pstK</name>
        <name type="ordered locus">MK1679</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:25037"/>
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            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.164"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the L-seryl-tRNA(Sec) kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.164"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUS5"/>
    <dbReference type="SMR" id="Q8TUS5"/>
    <dbReference type="STRING" id="190192.MK1679"/>
    <dbReference type="EnsemblBacteria" id="AAM02892">
        <property type="protein sequence ID" value="AAM02892"/>
        <property type="gene ID" value="MK1679"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1679"/>
    <dbReference type="HOGENOM" id="CLU_1100964_0_0_2"/>
    <dbReference type="OMA" id="RESFPVW"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00897"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043915">
        <property type="term" value="F:L-seryl-tRNA(Sec) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020024">
        <property type="entry name" value="L-seryl-tRNA_Sec_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03574">
        <property type="entry name" value="selen_PSTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000285593" description="L-seryl-tRNA(Sec) kinase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="255" mass="29790" checksum="DC44E95EDF2A58B2" modified="2002-06-01" version="1">MRLLILTGPPGSGKTCFARELARELRQEGWRVAHVEADALRGFLWDEFDPKLEQVARELFLKSVETCLDAELDLVIADDTNYYSSMRRELALLALERKVPWGIVYLRTGLDTCLRRNRERGEPIPEEVVRRIYDRFEPPEPDRWWERATLVLDDSRVSEEVLEFVESGLRVEKPKKRRRRTDPSSVNEVDVRTRQVMGELMRRLSETGAATQELGRKLSELRREIVSSVEDPEKAVREFRRRAEEVIRECLHGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS6</accession>
    <name>Q8TUS6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ERCC4-like helicase-nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mph1</name>
        <name evidence="3" type="synonym">mus81</name>
        <name evidence="3" type="ordered locus">MK1678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1678"/>
    <dbReference type="EnsemblBacteria" id="AAM02891">
        <property type="protein sequence ID" value="AAM02891"/>
        <property type="gene ID" value="MK1678"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1678"/>
    <dbReference type="HOGENOM" id="CLU_002513_3_1_2"/>
    <dbReference type="OMA" id="HRAVGNY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="PTHR14025:SF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="347"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="741" mass="84425" checksum="685FBB9BBE476530" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV0</accession>
    <name>Q8TUV0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02866">
        <property type="protein sequence ID" value="AAM02866"/>
        <property type="gene ID" value="MK1653"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1653"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1817"/>
    <dbReference type="HOGENOM" id="CLU_992515_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="280" mass="31413" checksum="4705F5F091E79F10" modified="2002-06-01" version="1">MKEFAVALHDKAPIYGLRIDNVYVSYSSANGVEYLYELSDNELKLLQQYRRIGEVPRGFRVCSELGLDTLIWIAGRDGSSYYELKRILLTENSTVRHVVYVNVSPNYLVTDDPSLPGYFSVNCYWNGPDKIWYPYYYLKLLGVEIVNVKDVYPTYSSTIQLVSLVDILADAALKALTKAVKHELVSQQPPEVKAEISHLLSALEGPEPPSPSALEEVMRKGGASEEFIESVLEGLRKYWSGSTGIGTSQRSPVNIIYTVLVLTLWVRRRPGGVARSRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV36</accession>
    <name>Q8TV36_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation initiation factor 2, GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB_1</name>
        <name evidence="2" type="ordered locus">MK1564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1564"/>
    <dbReference type="EnsemblBacteria" id="AAM02777">
        <property type="protein sequence ID" value="AAM02777"/>
        <property type="gene ID" value="MK1564"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1564"/>
    <dbReference type="HOGENOM" id="CLU_373259_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="2"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="744" mass="81437" checksum="692C8ABAB9A093B0" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV42</accession>
    <name>Q8TV42_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Selenophosphate synthetase-related enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1558"/>
    <dbReference type="EnsemblBacteria" id="AAM02771">
        <property type="protein sequence ID" value="AAM02771"/>
        <property type="gene ID" value="MK1558"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1558"/>
    <dbReference type="HOGENOM" id="CLU_073250_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011414">
        <property type="entry name" value="UCP036541_AIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036541">
        <property type="entry name" value="UCP036541_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="44"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="178"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="302" mass="32655" checksum="6E2AC40834FF6A20" modified="2002-06-01" version="1">MSARGAPSYEDLRRHVLRYTVDDVKVVRLLPEFGRAEVGLADYDDAAVVRVDGKLVVSSDGPYAFRLVRKSALVHASTDVLVAGGEPRFAVDTIIAPTEKGALEAARRIGRQARALGIEILGGNTMIEDDVEEPKVSLTVMGPLVAPEPITDCGAEPGDSVLLVGEPIHGSFQERMERARRLFDTFPELARRGLVKAAKDVTKGGLVAMAALVCAKSGVGMDLNSVPYSSITRNWDNVLAVVSPDDVEEVLNVCAERGCPVTMLGEVIEEPVLRIAGRTLVDSELMAEIEDHFKTFKNFKKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV60</accession>
    <name>MOAA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="1">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA</name>
        <name type="ordered locus">MK1540</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV60"/>
    <dbReference type="SMR" id="Q8TV60"/>
    <dbReference type="STRING" id="190192.MK1540"/>
    <dbReference type="EnsemblBacteria" id="AAM02753">
        <property type="protein sequence ID" value="AAM02753"/>
        <property type="gene ID" value="MK1540"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1540"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1701"/>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000153018" description="Probable GTP 3',8-cyclase">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="245"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="307" mass="34238" checksum="4B27148C66FF02A4" modified="2002-06-01" version="1">MRDALGREVRSVRISVTMRCNMACVYCHREGERPGRSELSAAEWGRLLRACAEIGVRKVKITGGEPLLRRDLIEIIENAEGFEEVSLVTNGVLLADYAGDLAEAGLDRVNVSLDTVDRKLYRKLTRSRFSPDDVIRGIEAAVSEGLTPVKVNVVLTSETVKTLPTLVEELADLEGLKLQLIEPMGSIPGFRPAHAEDGLRALGEYEPELERVRTFHSREVYRLNNGMAVEVVKPMDGVMCEACTRIRLTHDGKYKGCLMAPPKPLPRDDFGELVRTLKEYVRTRDDTFEVHQGTSVMGRMRGDVSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV74</accession>
    <name>Q8TV74_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02732">
        <property type="protein sequence ID" value="AAM02732"/>
        <property type="gene ID" value="MK1519"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1519"/>
    <dbReference type="HOGENOM" id="CLU_1665514_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="158" mass="17363" checksum="51951EA0F3C2B0AB" modified="2002-06-01" version="1">MARVLSEAIKRSGRTVGVLMLPQSTGGSGRKWVGGYRWDCDVVVATVDRVSECDYALILSRFGACTNARKAVYDLSHQSFVGFEPPEGWVGIDCFRVARDKRVPAVHALILLGAMCRIARLCELEDVKKALLSEEGRLGAVSYRAVRAGWEEAGRWGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV81</accession>
    <name>Q8TV81_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted serine protein kinase homologous to HPr protein kinase, contains a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1512</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1512"/>
    <dbReference type="EnsemblBacteria" id="AAM02725">
        <property type="protein sequence ID" value="AAM02725"/>
        <property type="gene ID" value="MK1512"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1512"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1670"/>
    <dbReference type="HOGENOM" id="CLU_1318545_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01918">
        <property type="entry name" value="HprK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003755">
        <property type="entry name" value="HPr(Ser)_kin/Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011104">
        <property type="entry name" value="Hpr_kin/Pase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30305:SF1">
        <property type="entry name" value="PTHR30305:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07475">
        <property type="entry name" value="Hpr_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hpr_kinase_C" evidence="2">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="208" mass="23128" checksum="2C5007356A714665" modified="2002-06-01" version="1">MERVVVHGTLMDVFGVGVLLVGPRGVGKSETALELIRRGHRLVSDDAVIVECDDSYEYPVGRPPENLAGKMEIRLALAELYCPECGRVYTFSALKMKEGCDCGGDLKLRPIQDKIAVDVAELYGIRALRSRKGIGVVIKMIPGPHHPHHDPSMRPDEPVSSTPSEEEKVVKWVKERDGRVFHLDVVPGRDTATLIETVALQESLRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD6</accession>
    <name>Q8TVD6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK1456</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1456"/>
    <dbReference type="EnsemblBacteria" id="AAM02669">
        <property type="protein sequence ID" value="AAM02669"/>
        <property type="gene ID" value="MK1456"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1456"/>
    <dbReference type="HOGENOM" id="CLU_063953_2_0_2"/>
    <dbReference type="OMA" id="HKLYMEY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="PTHR12209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM02669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="206" mass="23879" checksum="635624571CF540CA" modified="2002-06-01" version="1">MEAGDVIALGAESLLVRHDWLGLLAVYKIRLPKPYRHPSLDERLRRLRTRREARALIRLPEMGVPTPTLYEVDLDLSLLITEYIPGRTLKQATESSFDPDHYRKLGKLVGRMHEHGFVHYDLTTSNILVSGDDLYIIDLGLSEDSDDPEDHAVDLRVFERCLESSHPEVKEEAWRAFLRGYREEREEATDTVLRALEDLKSRVRYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE3</accession>
    <name>Q8TVE3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Demethylmenaquinone methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">menG</name>
        <name evidence="1" type="ordered locus">MK1449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1449"/>
    <dbReference type="EnsemblBacteria" id="AAM02662">
        <property type="protein sequence ID" value="AAM02662"/>
        <property type="gene ID" value="MK1449"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1449"/>
    <dbReference type="HOGENOM" id="CLU_072626_3_0_2"/>
    <dbReference type="OMA" id="MKLCGPA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="21374" checksum="9C32EF5553580635" modified="2002-06-01" version="1">MEVPPSPVLSDALGKRYVLRGIRHIVGPVPICGRAVTVLVEGTDWGTVVEAIDEAEEGDVLVIQARDRARAYWGGLSSLSAKRKGLAGTVVDGLVRDVDDVREIGYTVFARGVTPRAGTHRRSGKINVDLYVSGVLVRPGDIIVGDESGVVVVPEEEWEDALKRAREILELEDDIRTDIAANRAWWEIVPDKLGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVF9</accession>
    <name>Q8TVF9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-terminal domain of molybdenum-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">modE</name>
        <name evidence="2" type="ordered locus">MK1430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1430"/>
    <dbReference type="EnsemblBacteria" id="AAM02643">
        <property type="protein sequence ID" value="AAM02643"/>
        <property type="gene ID" value="MK1430"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1430"/>
    <dbReference type="HOGENOM" id="CLU_125440_2_1_2"/>
    <dbReference type="OMA" id="LRVWIEQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="105" mass="11692" checksum="08B93A3496767872" modified="2002-06-01" version="1">MECRVKVFLCADGRPVMGPGRYALLKAISEEGTVKGAAERLGWSYGYARRSIEALERAFGRKVVQTERGGPEGGRASLTDFGRKLVEEYERAMKEVREKGLKPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH8</accession>
    <name>Q8TVH8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ornithine cyclodeaminase, mu-crystallin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1411"/>
    <dbReference type="EnsemblBacteria" id="AAM02624">
        <property type="protein sequence ID" value="AAM02624"/>
        <property type="gene ID" value="MK1411"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1411"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1566"/>
    <dbReference type="HOGENOM" id="CLU_042088_3_1_2"/>
    <dbReference type="OMA" id="AVKAFTY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35305" checksum="32FAF80B0FAED5B5" modified="2002-06-01" version="1">MLFLSEDHVRALLDVREVVERVEETFRIKPECEMPPKTIVPLEGGDFRVMPAYLSELGVAGVKIVNSHPDNPDRGLPTVMAVMCLIEPETGRPLCLVSATEITSLRTGAAGAVASKYLAEDVRTVGIVGAGVQGRYQLLTHAEVFDLEAVFVYDQAREAARRLAEWVERDLGVDAKVLDNLDFSGLDVDVVCTCTPATEPYLGSEDVPEDVHVNAIGADAPEKQEVKTELLKRAVVVVDDREQCVESGDVSQPVERGELNPEELIELSDVVRGETEVDSSELTVFDSTGIAILDVAVGALAYERAKKTDAGVEVKPFELPTGNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK4</accession>
    <name>Q8TVK4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogenase molybdenum-iron subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nifD</name>
        <name evidence="2" type="ordered locus">MK1385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1385"/>
    <dbReference type="EnsemblBacteria" id="AAM02598">
        <property type="protein sequence ID" value="AAM02598"/>
        <property type="gene ID" value="MK1385"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1385"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1540"/>
    <dbReference type="HOGENOM" id="CLU_782144_0_0_2"/>
    <dbReference type="OMA" id="CASMIIG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017675">
        <property type="entry name" value="CfbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03282">
        <property type="entry name" value="methan_mark_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_nitro" evidence="1">
        <location>
            <begin position="25"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="349" mass="38156" checksum="6E8644FA14EC1CEE" modified="2002-06-01" version="1">MSTWHPRPGPIPAAMYTLRDLLADAVVLHGPKGCCFRTARLLEKDGVRVFVTGMEEDDFVFGALEKLVELLEYVEERLEPELIGVVGTCVSSIIGEDLEAAVEEADVDATVVTVEVHNGMGPNTEGVIRTLERAAEAGVIPEGEVERQKRLMRAAAELERRRGMASREYLEPWSGHDPSEVARVLLSSEDVLAILNAKKETAYLFADPVLEVGKRGAWVLANLSPESGLPKVRRDAEVIGSIFREEGIEFEVTGSLDEYAVTGELLAEKIEEFDPDSVLITGIPHAVAPEELDVDATFVAVTDGLREASALRELGYDYVVVEEEAHARVLGRREIVPSDLGEAIRQLSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL9</accession>
    <name>TYSY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative thymidylate synthase</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyA</name>
        <name type="ordered locus">MK1370</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May catalyze the biosynthesis of dTMP using an unknown cosubstrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVL9"/>
    <dbReference type="SMR" id="Q8TVL9"/>
    <dbReference type="STRING" id="190192.MK1370"/>
    <dbReference type="EnsemblBacteria" id="AAM02583">
        <property type="protein sequence ID" value="AAM02583"/>
        <property type="gene ID" value="MK1370"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1370"/>
    <dbReference type="HOGENOM" id="CLU_084975_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01686">
        <property type="entry name" value="Thymidy_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03283">
        <property type="entry name" value="thy_syn_methano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141056" description="Putative thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01686"/>
        </source>
    </evidence>
    <sequence length="209" mass="23781" checksum="08A0674BBBA8AB39" modified="2002-06-01" version="1">MLGKGRPLVVRGRTVEQVWRQAVTGIKVHGEKVERERGPVKEVRGLIAHLEPSGPESFDIPDDYPLDEHSVRAYEDQLLDPELRGFEYTYGHRLRRYFGLDQVTKIVERLSESNNTRRAIAVTWDPRRDLDEEEVPCLTALQLQSDGGSGLELHAFYRSWDVGKALVANMIALRRLQEHVAERAGLEPTTLTVYAANAHVYEEDLPDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN0</accession>
    <name>Q8TVN0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative component of a threonine efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02571">
        <property type="protein sequence ID" value="AAM02571"/>
        <property type="gene ID" value="MK1358"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1358"/>
    <dbReference type="HOGENOM" id="CLU_104651_1_0_2"/>
    <dbReference type="OMA" id="PHPYVFW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="20761" checksum="E40E2D1EA5DFC48D" modified="2002-06-01" version="1">MHHTASEMLAVGAALGVSGGLAPGPLQALIVAETLGNGLRAGLAVAVVPVITDGPLVTAAGLAAARLPGWVLRYLGLAGSAVLAYMGLSLIREADSAEPARSEGAGSSLRRAVVVNLLNPHPYVFWLTVGSSMMGSARSLPEMVAFPVGFFLGIVSTQAGIAVAVHRALSLTPAERVGGARRLSGVLLLGAAAYLAYVSLRDMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN9</accession>
    <name>Q8TVN9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK-2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02562">
        <property type="protein sequence ID" value="AAM02562"/>
        <property type="gene ID" value="MK1349"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1349"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1494"/>
    <dbReference type="HOGENOM" id="CLU_1140572_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="27269" checksum="319C3C53DC9BBBE6" modified="2002-06-01" version="1">MADYLEALSCLKSHDLDRCRLVADDLPIHYLTLIPSADLLTDNRPQTFLERNELRVQTSFGEFHVGLRTPAVVSGCLENVLLRLGAPAPVTLLRDGTAAFVSPIGVIFARLDGRVVPEAEIRYEKYWRSASNFFRAPSADYYEYRFDARRGVAEVRFYDLERRIAVTVSLNFNGKSATFSESAIDETTLRELRSRLKRVSEVQDAVLVRTPDGTEVVWVEFGVPGPPPGLTSRDGTEDPQHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR8</accession>
    <name>Q8TVR8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted xylanase/chitin deacetylase family enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02533">
        <property type="protein sequence ID" value="AAM02533"/>
        <property type="gene ID" value="MK1320"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1320"/>
    <dbReference type="HOGENOM" id="CLU_1080146_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="56"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="28709" checksum="D1C908497DA88010" modified="2002-06-01" version="1">MSRPRPGSRGWMLMSEGVFALIVILLTFTATVGSIHFKHTHPRKQNRLTRLSHARCIVVVTEDLCGGSRYSVRLAEMLRRRGVPMTFAVPAWEAYAYPDRMRKLRELGGDLINHANDGGASYAFLGRPGKVAGLPVKTQRQIIHTTQKWIHRATGVRPDAFRAPGLAIDENTYRALVEEGIWVDSSKIYTRPGTRGGAQVDRDRRPPDPRGTHSVLRGVAVRARAAGGAGVPVLVRRRPVRASQDSDRSPPRRRWRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT1</accession>
    <name>Q8TVT1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted nucleotidyltransferase of the DNA polymerase beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02520">
        <property type="protein sequence ID" value="AAM02520"/>
        <property type="gene ID" value="MK1307"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1307"/>
    <dbReference type="HOGENOM" id="CLU_2340195_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="97" mass="11269" checksum="4684A0F1573E5138" modified="2002-06-01" version="1">MTRWRYWAERIAEVARDLLGGRTRVYASVEGDRLRVVIVSGNAPEKPLERAEIVAEIERELGLEESWAHPIEMHVVDPEEYEALWRGVLREAVEVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT6</accession>
    <name>Q8TVT6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02515">
        <property type="protein sequence ID" value="AAM02515"/>
        <property type="gene ID" value="MK1302"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1302"/>
    <dbReference type="HOGENOM" id="CLU_2581434_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="80" mass="9515" checksum="3A76CA67D1ED19DE" modified="2002-06-01" version="1">MECAWKVYDRLRNESENKRRMGHYLGIGRRYSRSRVHFQVKRLKNGEFVPVVTAFAGRRKERGNLKGVRKAMGKSWTLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT9</accession>
    <name>Q8TVT9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted component of a thermophile-specific DNA repair system, contains a RAMP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02512">
        <property type="protein sequence ID" value="AAM02512"/>
        <property type="gene ID" value="MK1299"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1299"/>
    <dbReference type="HOGENOM" id="CLU_047795_0_0_2"/>
    <dbReference type="OMA" id="WVGGKET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013410">
        <property type="entry name" value="CRISPR-assoc_RAMP_Cmr4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36700">
        <property type="entry name" value="PTHR36700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02580">
        <property type="entry name" value="cas_RAMP_Cmr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="342" mass="37086" checksum="FAD1F020A5C38A6D" modified="2002-06-01" version="1">MTDALPYFLVCRTPTRAGAGQRATDVIDLPLQREAHTKLPVIYGSTLKGALRHATLRKLSEELDGETSEGLVDAVFGDRPGEGSPSPGVVAFSDAVLLAMPVRCEPGFLAWVTSPYQLGRLYEVLELTGELEDLREAVEEVLNDCKDPRGNGALAPEEGTLLLDRIRVRAEASDAVGDLAGVLSETVFEGAPEPHFRRYVEERLVVLGDGAFADLVNSCTERVVRVRLNEEKTVEQGPWYEERVPEGTVFFGTLNVRHDTVPPKNGVDARKVLFGRWEPDGVPDGDDRKLEALKEAAGELEAGVLGAVADGSGDGYLDLRFQVGGSETVGFGLVRLRQFVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV7</accession>
    <name>Q8TVV7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein specific for M.kandleri, MK-14 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02494">
        <property type="protein sequence ID" value="AAM02494"/>
        <property type="gene ID" value="MK1281"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1281"/>
    <dbReference type="HOGENOM" id="CLU_1329477_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="206" mass="23838" checksum="9F894AA5B696BD44" modified="2002-06-01" version="1">MGVPPTSWYHVIHVLAILVVLIAISQFLGTLIITRRSRRLRSKAERILEIVEKAEESSDAVFRGEGTAEELDRELSELLKIPDTSVALRFGIAMMNVWETKGREDALDEALRLIDSESLEAFEPYLIPFDVRLRTTHVRMSTWNRIRGLIVPLGWSVQRSKRRGRPIAPERTTRDRPLRGMSLGFGRHRSGRPETPRVRVRWDYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX0</accession>
    <name>Q8TVX0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-28 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02481">
        <property type="protein sequence ID" value="AAM02481"/>
        <property type="gene ID" value="MK1268"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1268"/>
    <dbReference type="HOGENOM" id="CLU_556245_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="490" mass="53061" checksum="EF667C3238A15691" modified="2002-06-01" version="1">MLPALLALAVLLALPGARALDLYPVEVVEVGTDAVGPPVISWDYPFERGPTVTYPSDGGWVRVRLDGSLRAREVVFDAAPPDLRGIAYLALERDGRVIAVDYLNRRVLEDRGAHPTVPTVSRSDSGAVVAYVRDGRLRAVLLGEEASVPLELHLRGEDPFVLVREGTVLVAYRSGGDVHLAVMGTEWSVRVRGSECVLLHREGDRWAEVLRWYTPERLTDADLLDLLKGRAPEERVVCGVPVELGSRTVWVFPLPLAPVVDAGTGAVVGWSHSAGLEPRELIGTVEEDGDVLVFTGWWEGLHASVAPAARLSGSRAIPGSLGLVGCERVMAVAFRDPDGHVRVAVPGGVRVAPTVVVPVETVDLGPGEPLAFLEGLVFYRTPGGDVRCAAVRPEGGPFVRPVVEEVPVRRWEVGGKELLVAGSVVLDPGTGRAYVLPSERSGEPVYRRDLSDGWFVAVFRFDDGFVVVLSDGRSVRESREYRSVTRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY9</accession>
    <name>Q8TVY9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02462">
        <property type="protein sequence ID" value="AAM02462"/>
        <property type="gene ID" value="MK1249"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1249"/>
    <dbReference type="HOGENOM" id="CLU_113705_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="125" mass="14409" checksum="3772F69BD82132AC" modified="2002-06-01" version="1">MPGLEEHLERWFKNESEAVTVYRTLAGLAEEAGMSEVAETFRKIADEELRHAAIVARIAGKLSEDIRAEVERLAEREAQAAELRRNVIEEYGDEMSEDVRAYLISTAHDEERHTKMLRNLLKRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ0</accession>
    <name>Q8TVZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ferrous ion uptake system subunit, predicted GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">feoB</name>
        <name evidence="11" type="ordered locus">MK1248</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1248"/>
    <dbReference type="EnsemblBacteria" id="AAM02461">
        <property type="protein sequence ID" value="AAM02461"/>
        <property type="gene ID" value="MK1248"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1248"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1352"/>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_2"/>
    <dbReference type="OMA" id="PLGYDWK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="622"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="10">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM02461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="650" mass="70453" checksum="427B5FF2001ECA4C" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW01</accession>
    <name>RFRNP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-free ribonuclease P</fullName>
            <shortName evidence="1">RNA-free RNase P</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein-only RNase P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1237</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-free RNase P that catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HARP family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW01"/>
    <dbReference type="STRING" id="190192.MK1237"/>
    <dbReference type="EnsemblBacteria" id="AAM02450">
        <property type="protein sequence ID" value="AAM02450"/>
        <property type="gene ID" value="MK1237"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1237"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1341"/>
    <dbReference type="HOGENOM" id="CLU_109672_0_0_2"/>
    <dbReference type="OMA" id="YIIKKPP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01078">
        <property type="entry name" value="RNA_free_RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014856">
        <property type="entry name" value="RNA_free_RNase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173">
        <property type="entry name" value="PTHR41173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08745">
        <property type="entry name" value="PIN_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03875">
        <property type="entry name" value="RNA_lig_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136080" description="RNA-free ribonuclease P">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01078"/>
        </source>
    </evidence>
    <sequence length="228" mass="26570" checksum="2AA73E88D1D352FA" modified="2002-06-01" version="1">MTVYKQRFVLDTTAFTDRGVVDELGDGDLCEAVTRLLDLIGRARMELAISCYIPYPTVYRELKGFLERNGCPELLSRIDTWIVKKTPNRYEVKVPARLFMAYVKDMRERLDRGRKRAEKAIWEAALEAYEIMLREEADVPKERIIREVIGETVRRFRRKYRQTVRHGTLDSAPDLDVLLLAKELDAAVVASDEGIERWARELGLRFMPACSFPDMIEEYLRMSREVEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW08</accession>
    <name>G1PDH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="1">G1P dehydrogenase</shortName>
            <shortName evidence="1">G1PDH</shortName>
            <ecNumber evidence="1">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">egsA</name>
        <name type="synonym">gldA</name>
        <name type="ordered locus">MK1230</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW08"/>
    <dbReference type="SMR" id="Q8TW08"/>
    <dbReference type="STRING" id="190192.MK1230"/>
    <dbReference type="EnsemblBacteria" id="AAM02443">
        <property type="protein sequence ID" value="AAM02443"/>
        <property type="gene ID" value="MK1230"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1230"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1333"/>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="OMA" id="ALHGEQC"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NADP+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000157343" description="Glycerol-1-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <sequence length="353" mass="37642" checksum="64A575C0A35AA30A" modified="2002-06-01" version="1">MSVPKKRMQLPREVVVGSNVLPEVPKLLRSVGVPDGVVAVFSGRTTMKIAGNEVADHLEEAGYQTSPVIVKGSTGDDVKKALEALDEIDADVVAAVGGGKVIDVAKVASYRRGIPFISVPTSASHDGIASPFASIRREGRPYSEPAQAPLAILADIEVIREAPERLIRAGVGDVVSNVTAVKDWRLAHRLRNEPYSEYASSLSLMAARIVMKNAKPIGKLLPEGIKKLVQALISGGVAMSIAGSSRPCSGSEHLFSHALDVIAERPALHGEQCGVGTIIMEYLHGGNWREIRETLETAGAPTTAEDLGVSDEEIIEALCRAHKIRPDRYTILGDKGLTREAAERAAEETGVIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-08-10" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW10</accession>
    <name>PSB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB</name>
        <name type="ordered locus">MK1228</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW10"/>
    <dbReference type="SMR" id="Q8TW10"/>
    <dbReference type="STRING" id="190192.MK1228"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="EnsemblBacteria" id="AAM02441">
        <property type="protein sequence ID" value="AAM02441"/>
        <property type="gene ID" value="MK1228"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1228"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1331"/>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="OMA" id="VDKTGPH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000397362" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000397363" description="Proteasome subunit beta">
        <location>
            <begin position="11"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <sequence length="210" mass="22828" checksum="EDF3596BF163ECA8" modified="2002-06-01" version="1" precursor="true">MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVADAQKIVDLMRAEARLYELRHNRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDDPALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMSLDDAIEVAVRAVKSALERDTGTGEGVTVVTITREEGYRELPEEEVEKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW11</accession>
    <name>Q8TW11_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted metal-dependent RNase, consists of a metallo-beta-lactamase domain and an RNA-binding KH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1227</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1227"/>
    <dbReference type="EnsemblBacteria" id="AAM02440">
        <property type="protein sequence ID" value="AAM02440"/>
        <property type="gene ID" value="MK1227"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1227"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1330"/>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="OMA" id="YLDGMIW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="KH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="206"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="435"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="652" mass="74081" checksum="FECBD7DC8CCE3671" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-19" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW17</accession>
    <name>RL5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl5</name>
        <name type="synonym">rplE</name>
        <name type="ordered locus">MK1221</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW17"/>
    <dbReference type="SMR" id="Q8TW17"/>
    <dbReference type="STRING" id="190192.MK1221"/>
    <dbReference type="EnsemblBacteria" id="AAM02434">
        <property type="protein sequence ID" value="AAM02434"/>
        <property type="gene ID" value="MK1221"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1221"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1324"/>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_L5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_L5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125057" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="186" mass="21656" checksum="9A5CD8ADC011BAC2" modified="2002-06-01" version="1">MSVVDEETRRKILEDWESNPMRKPRVGKVTVNIGVGESGDRLQKAYELLQELTGQKPVYTRAKQTNPSFGIRRGQPIGVKVDLRREQAIEFLDWTLDAVDRELHESQFDEFGNVCFGLEEHIALEGVEYDPEIGIFGMDIAVTLERPGFRVMRRRRCRRPVPRRHRLTKEEGIVFMEEEFDVEVLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW29</accession>
    <name>LEUC2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-isopropylmalate dehydratase large subunit 2</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM isomerase 2</fullName>
            <shortName evidence="1">IPMI 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC2</name>
        <name type="ordered locus">MK1208</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW29"/>
    <dbReference type="SMR" id="Q8TW29"/>
    <dbReference type="STRING" id="190192.MK1208"/>
    <dbReference type="EnsemblBacteria" id="AAM02421">
        <property type="protein sequence ID" value="AAM02421"/>
        <property type="gene ID" value="MK1208"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1208"/>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076871" description="3-isopropylmalate dehydratase large subunit 2">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <sequence length="402" mass="43096" checksum="D7311C9C7BB2047A" modified="2002-06-01" version="1">MPSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVLIFDHSVPPSSVEAANRQNELLEFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDSHTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVHVEGELEEPATAKDLALTVVGELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEVIELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDGPHPDVRIVVVPASRRVLERMTEAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVSTANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW34</accession>
    <name>Q8TW34_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02416">
        <property type="protein sequence ID" value="AAM02416"/>
        <property type="gene ID" value="MK1203"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1203"/>
    <dbReference type="HOGENOM" id="CLU_1438120_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="20527" checksum="A2963B076898C8BC" modified="2002-06-01" version="1">MVDNLYDQFVKYHGDAQALVAVFTGKSVHLATVCVGGYAGDVEKLSGLYSTVRTRPESMRSFLESVIPTLWKYLSMHLPDDVGDRPAHMISSGTPFYSGLASGYVHSYLAASGGVLIADTKDPGSVERARLVPFYPERVGKITLARGSNLDERCYSYSEVGYYEWNGTKYACEAGPLTRFSGSLQGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW66</accession>
    <name>Q8TW66_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fusion of at least two uncharacterized domain specific for M.kandleri, MK-12 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02383">
        <property type="protein sequence ID" value="AAM02383"/>
        <property type="gene ID" value="MK1170"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1170"/>
    <dbReference type="HOGENOM" id="CLU_620572_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019692">
        <property type="entry name" value="DUF2581"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10756">
        <property type="entry name" value="bPH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="383"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="441" mass="47511" checksum="A7F01BCA5EE3E8AC" modified="2002-06-01" version="1">MTEPLRPPELEDPKRVYVPVAGAGIATAVCLYLILPRGLFPALLALAVTAILLGVGTLVFRRYLGNQVELTPDAVILKGPGGTRRIPVEDILDVEERESGVVVLTKDLERVPMPFDSDRLRHGLRILLAHKKGSPGDAVVEAVRELGELPILEVVGGTVFARWRWLRVPLYSPGDPRLEEFHDRVLEAWMECKGLGKKPPSPEMVAVTAILPTVTPILAMRKGAKTSPSGRDYLGKLIHWILVKVAKALKVSLWAAATIAAAIYFGPGATVSAVYWAIARRVDSVPVPKSRIKHATLTFLEYNALLTGLLAPVIGILYLPLPRVHCPRLAIALALGAVATMAPSVRAVSHYRRDPVGYTAALLNDGILVPEVVLIGAGTSVLLLVLVGAVPAALTIVATFLFHAVIVLRRIERAAELSRRAWKLRVSRDGTGGNIPHTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA0</accession>
    <name>Q8TWA0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02349">
        <property type="protein sequence ID" value="AAM02349"/>
        <property type="gene ID" value="MK1136"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1136"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="209" mass="24477" checksum="86A8E55E7BED3562" modified="2002-06-01" version="1">MPELVESHVHVPIPSDPVERIRALRVLREVYRRGKKPSLEVTYRTVGGSTCGPYYVARWRKDSRFKHGRTLYLDKSENESVSFVEWLVSLDRSEVLELARHLMRNLRSVLKTLLTEVLSLPYKKARRVLTRGLALAFDARPSNSPRIRDLLEELPDRLESFAVRTLGGWPAHYSSYLRKVIRSRRRSLDGRHEVPDVELELERWKLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC2</accession>
    <name>Q8TWC2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-22 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02326">
        <property type="protein sequence ID" value="AAM02326"/>
        <property type="gene ID" value="MK1113"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1113"/>
    <dbReference type="HOGENOM" id="CLU_1346414_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="203" mass="22778" checksum="F321D983E3B911BD" modified="2002-06-01" version="1">MHELLTFLLTVLSPVAGLVDGGSMYLTPDVHTVVGTQWDVTRVVLTTPELDEGAVFVREGDRWRETMEETSTRFGSLRRLDDVRVRWSGGLLHVQCPKSAEVLLLVPDPEGIPVTEGSTFLEGGPLPEGAHVLLGGCVRIRRYRGPVVLEIPFETARESIVSVRWIEGTTYYEYTDWAAHDWDRRFRPAFLLPALVSCVRRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF0</accession>
    <name>Q8TWF0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Adenylosuccinate lyase</fullName>
            <shortName evidence="9">ASL</shortName>
            <ecNumber evidence="5 9">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">purB</name>
        <name evidence="11" type="ordered locus">MK1085</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="5 9"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1085"/>
    <dbReference type="EnsemblBacteria" id="AAM02298">
        <property type="protein sequence ID" value="AAM02298"/>
        <property type="gene ID" value="MK1085"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1085"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1140"/>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="10">
        <location>
            <begin position="369"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM02298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="472" mass="53803" checksum="1E84667F098B0794" modified="2002-06-01" version="1">MPVHPIESRYGSDELRRVFSEENKVAKMLEVEAALVRALSEVFDFVPEEAADEVERVVEEITGDEEELRRFVERVKEIEAEIKHDVMALVKALSERCEVGGDYVHLGATSNDVIDTAHALVLREALSIIYRRLHRLAEVLAEKAEEYADLPMVGRTHGQHAVPTTLGMKFAIWAREVVRHLKRLRECANRVLVGQLSGAVGTMAALGEKGPEVQRRVMELLNLRPVTVSNQVIQRDRYAELIALLALIGSTLDKIGREIRNLQRTEIREVEEPFDPEKQVGSSTMPHKRNPIRSERVCSLARVLRSNVQIALENVPLEHERDLTNSASERVILPEQFLLLDEMLRLTIHNLEGLRVYEENIRENLRLTKGLNMAEALMVELVKRGIGRQEAHELVRRLAMRAWEEGRDFAEVVKEEERVRELFGEEELDEVLDPEKYLGVAPDLAREAAEKTRRDLEEIDREMKEVLGVGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ3</accession>
    <name>Q8TWJ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02254">
        <property type="protein sequence ID" value="AAM02254"/>
        <property type="gene ID" value="MK1041"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1041"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1094"/>
    <dbReference type="HOGENOM" id="CLU_244882_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1559"/>
            <end position="1577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1586" mass="177484" checksum="8CBE6D3DCFD6E7CD" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ6</accession>
    <name>Q8TWJ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-2 methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cobF</name>
        <name evidence="6" type="ordered locus">MK1038</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1038"/>
    <dbReference type="EnsemblBacteria" id="AAM02251">
        <property type="protein sequence ID" value="AAM02251"/>
        <property type="gene ID" value="MK1038"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1038"/>
    <dbReference type="HOGENOM" id="CLU_076014_2_1_2"/>
    <dbReference type="OMA" id="MEYPVTT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="220" mass="24389" checksum="270F2BAA760CCE1B" modified="2002-06-01" version="1">MNTGAPTLYGVGLGPGDPDLLTRKAIRIIKKVPVAMVPFVGAESRAARVVRAVDPEATVVGYHAPMTRDPEERDRAYSKAAETLAELLERFHEVAVCNLGDPTLYATFWHIVERIPLEDFEIELVPGIPAGLLCAARIGRPLAMESDKVLICTREPPEDLEGMDTVILYKPTRRGVERLLREGFEVYACRELGFEGERVERVNGAEFEPSYLCTIVALRP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL5</accession>
    <name>TRMY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmY</name>
        <name type="ordered locus">MK1018</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWL5"/>
    <dbReference type="SMR" id="Q8TWL5"/>
    <dbReference type="STRING" id="190192.MK1018"/>
    <dbReference type="EnsemblBacteria" id="AAM02231">
        <property type="protein sequence ID" value="AAM02231"/>
        <property type="gene ID" value="MK1018"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1018"/>
    <dbReference type="HOGENOM" id="CLU_107018_0_0_2"/>
    <dbReference type="OMA" id="HADHCII"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="PTHR40703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000157949" description="tRNA (pseudouridine(54)-N(1))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <sequence length="203" mass="23033" checksum="C292AB7DCE66BBBF" modified="2002-06-01" version="1">MREFLVLFNHAPTSPDRVRLKDLPGSGRFDLVCRVTTQALLYSHGVRTDTVVHLLLRGPDDPPKTITVTGRRVRRLYPDERTTAIHLRRALEADPDTEPHPGIFVRRADLEDLLGEMKGAKLYYLSEDGRDLEEVEPEPDAVFVLGDHEGPTPEQDRLLRRHADAVISLGPIPYHADQCIVILHRYLDVKRPPEYAHGPSNVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM2</accession>
    <name>Q8TWM2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD-binding component of a K+ transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trkA</name>
        <name evidence="7" type="ordered locus">MK1011</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1011"/>
    <dbReference type="EnsemblBacteria" id="AAM02224">
        <property type="protein sequence ID" value="AAM02224"/>
        <property type="gene ID" value="MK1011"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1011"/>
    <dbReference type="HOGENOM" id="CLU_046525_2_0_2"/>
    <dbReference type="OMA" id="RWTADQM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0630">Potassium</keyword>
    <keyword evidence="2" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="6">
        <location>
            <begin position="138"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="222" mass="24540" checksum="01C284BB6A9696D8" modified="2002-06-01" version="1">MYVVVVGGGRVGRILAELLIAHGHDVVVIEKDKRRARELAQSELDVLVVQGDATDLDVLQDAEIETADVVAAVTDKDEVNLAVSLIAKEEFGVERVVSRVTDDRFVDVFRRLGVDAVVNPDRSAAQLIEKAITRHNLVDVIMFGRGEAELLEFEVTEDSLVSGKKISEIPADCVIVAIYRDDELELPRGDMEINPGDRVLVLALRDKLKKVEKLFKGRDEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM9</accession>
    <name>TIAS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="1">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="1">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tiaS</name>
        <name type="ordered locus">MK1003</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWM9"/>
    <dbReference type="SMR" id="Q8TWM9"/>
    <dbReference type="STRING" id="190192.MK1003"/>
    <dbReference type="EnsemblBacteria" id="AAM02216">
        <property type="protein sequence ID" value="AAM02216"/>
        <property type="gene ID" value="MK1003"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1003"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1052"/>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="OMA" id="GMCTTYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000407297" description="tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <sequence length="433" mass="47948" checksum="2DECF42DAB5664DC" modified="2002-06-01" version="1">MDWVALDDTDSPAGGCTTHAAVLLRAELAEAGAEPVGRPLLVRLNPNVPFKTRGNAAVALPVEAPWSVDIEAVVLRALKKVVRKGYPETRPGLVVCEGEPPRVCESVYEEAVRRILNPGRVKESVDDDVNVVLEGRGIVGAVAALGFARVRKDHEVTFEGIAYRAEKYWGTERRVEESSIREFDRRTFPVTFDNLDSRDGDVLITPNTPCPVLYGVRSVEPDVLEVAPDMIKTREPVVEYEIFESNQATDAHLVRVDRLADAEDYSNPVLDLTVVEEPRRIPGGHVVVRCEDEEGVRVDIAAFRPARPLTEVVAALHPGDEIRVAGALRPETPKHPRTVNVEKLRVLRLERVEEVRNPVCGRCRRSMKSAGRKKGFKCSCGERAPEDSKIGVEVPRELVEGVTYEAPPVARRHLSKPEYLVELGLLEPSPLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS5</accession>
    <name>Q8TWS5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted Zn-dependent protease of TldD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tldD</name>
        <name evidence="5" type="ordered locus">MK0957</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0957"/>
    <dbReference type="EnsemblBacteria" id="AAM02170">
        <property type="protein sequence ID" value="AAM02170"/>
        <property type="gene ID" value="MK0957"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0957"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1002"/>
    <dbReference type="HOGENOM" id="CLU_026425_1_0_2"/>
    <dbReference type="OMA" id="ENGETEH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="214"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="445" mass="48229" checksum="83900B4E6A2A5A95" modified="2002-06-01" version="1">MDPDVLLRHAEEISDACVVYALEGELVEIEASNGELRKADSDRVRTYAVRVLKEGSWGVASGPDPERDLVERALRSTGEGSAEIPEEVPAAEGSYRWEGKLSPLDSLDEAAELAVELSREVSYDCEITYSAGSVRYTITSTWGSECEVRLDCVNFGVKVSGKGTAGREEYTERDGANCAGLELFLERAEEVRDEAVRRLEDLLEAEPGPERAESVITDPELLGVIVHEAFGHAVEGDLVARGESVLQDWVGERVASEIVTVVDDPTERGAFGSYPFDDEGVEPRRTVLVEEGVLRGYLTDLTSAAELDLEVTGNGRLESIGDHVQVRMSVTYVEPGDASREELFEEAGDGAVYLLGSKGGQTDTATGNFQFSAKLGYVVEEGEPSRPVRDVGLTGETLEFMKRVRMLSDELRLHPGYCGKGGQLVPVSDGGPHALVDGPFHLRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT6</accession>
    <name>Q8TWT6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-4 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02159">
        <property type="protein sequence ID" value="AAM02159"/>
        <property type="gene ID" value="MK0946"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0946"/>
    <dbReference type="HOGENOM" id="CLU_2257385_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025328">
        <property type="entry name" value="DUF4234"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14018">
        <property type="entry name" value="DUF4234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4234" evidence="2">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="12033" checksum="78F13F0C9568CEDB" modified="2002-06-01" version="1">MISHVGIVTIIILISKLISPSRKRKFYRTLAFVYAVLGFIPPFAWLLGGFACHRINLDLRERGCASERHTPRYAVLTILTLGLYQSYWWYVTTEDVNRCLRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV6</accession>
    <name>Q8TWV6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-36 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02138">
        <property type="protein sequence ID" value="AAM02138"/>
        <property type="gene ID" value="MK0925"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0925"/>
    <dbReference type="HOGENOM" id="CLU_854215_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35863" checksum="EBD6B34ADE7E53E4" modified="2002-06-01" version="1">MRWTPALTAIVTLLAPVSSTMVNVAMWAGDGAASNCVESSLRAIEYWNANYAGTYHINVITYASPSDWRTVLETGRDPYTGTHIDVIYVPGGYHPEWHWDPDWIDLLYHAQIDLGIGYVGICAGAYLHAGVTHRGAIGEDLVVDGVTAIDGNRGNGYVSVLIQQNPITPQSTWGKVYEYYYANGPGMAAEDGSVFISDNRWYKVCWLDGKEVRINVQGFGKYVELVSGWAMVLGWYNVKKGDKWEPRGRFVLLGVHPELTERTGAWKLLARALIWAAGYEPKETPTKKQGPSEQTPLVPVPPICSPRSRRACGRSPRATPSCQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW3</accession>
    <name>Q8TWW3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted phosphosugar isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">agaS</name>
        <name evidence="2" type="ordered locus">MK0918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0918"/>
    <dbReference type="EnsemblBacteria" id="AAM02131">
        <property type="protein sequence ID" value="AAM02131"/>
        <property type="gene ID" value="MK0918"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0918"/>
    <dbReference type="HOGENOM" id="CLU_923201_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="22"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="301" mass="33180" checksum="EA5E5EFBB7E05F7B" modified="2002-06-01" version="1">MRDDVERTPDDLRAVLTLGFEDLPRLSSYQFILTTGSGSSHHVAHYASLLFTHELNIATVSHPGRLAAWADGADLAIVVSMSGGKDSHSIARALGCEILAVTCEENSPLADLADYLLLLPVEREEGFLNTRTIVSAMFALCAYASELSGEDLVDPDVPDRVERRLERGVPEDVILEMRDREKVFFIGDKYLYVAAEQAALKSIEVGDVNAFATTSDELFHGRFFGDHSERLYLCLNEKGADLIEERSGGEATVVRPEDLGLDVDPEDPNAPVETLPVLYLLVDEAYGPVDTTEARSWWNHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX2</accession>
    <name>Q8TWX2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02122">
        <property type="protein sequence ID" value="AAM02122"/>
        <property type="gene ID" value="MK0909"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0909"/>
    <dbReference type="HOGENOM" id="CLU_929418_0_0_2"/>
    <dbReference type="OMA" id="MVAYEGF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036983">
        <property type="entry name" value="AIM24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016031">
        <property type="entry name" value="Trp_RNA-bd_attenuator-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51219">
        <property type="entry name" value="SSF51219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="299" mass="32443" checksum="05A56E442F7201CE" modified="2002-06-01" version="1">MCDARRGCSTLGLLDSLLGGGDEGSGEAEVREVRDEVIEVELIRGPVYFKNDSIIAARGDLEFLATKPVRSSGGITGLLEKVLKAKLLGLRRYFVKVDGSGVLYAGDGGKVKVLEVPEGETVTVNQDRLLFTTTPADVAAQLDASVLSTGLIDMGFEGPCKIVVTAECDPVTVEVDGGPVYVDPECLIAWTGDLTFSATWKGSAFDFLLGREHGEEFLLEAHGYGKVLVAPYDERIRRLERMIRRAGARGGGVVGGGENYGEEYGEEYEEYEESSDEGGLFDDLFDFDFDTDDFDLDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX4</accession>
    <name>Q8TWX4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prdicted regulatory protein consisting of a uncharacterized conserved domain fused to a CBS domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0907</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0907"/>
    <dbReference type="EnsemblBacteria" id="AAM02120">
        <property type="protein sequence ID" value="AAM02120"/>
        <property type="gene ID" value="MK0907"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0907"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.949"/>
    <dbReference type="HOGENOM" id="CLU_043239_0_0_2"/>
    <dbReference type="OMA" id="WVRGCYF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004698">
        <property type="entry name" value="UCP004698_CBS_MJ0100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="386"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="447"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="501" mass="55437" checksum="3B634AF30DD6E532" modified="2002-06-01" version="1">MPGRKSIDEINRRIERGEVCVVTAEEMVEIVEDIGPERAAKEVDVVTCATFGPMCSSGVFLNFGHSDPPIKMRRVWLNEVEAYTGIAAVDAYLGATQPSEDRGIEYGGAHVIHELASGEEVVLRAEGSVTDCYPRESVETLITIDDINQAVIVNPRNSYQRYVAATNSSEETLYTYMGKLLPEYGNVTYCGAGQLNPLANDPEFRTIGIGTRIWLAGSHGYIIGEGTQHDPSSGMATLMVRGDLKEADPEYLRPVVLEKYGVSLAVGIGVPIPVLDERVAASTGVSDADIEVPIVDYGIPSRDRPVVKRVTYEELRSGKVEIEGRTVRTGALSSYKMALEIAERLKEEIEEGEFTLTQPVEPLPREREFRPMPYRPPSLPRVRDIMTESVVTASPDESIEDVARRLIEKEINHIPVVDEEGRIVGIVTSWDIAAAVAEGKRRLKDIMTEDVITIRPHESVDEALRRMDRHNISCLPVVDGENRVVGIVTRTDITEVLRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX04</accession>
    <name>Q8TX04_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02090">
        <property type="protein sequence ID" value="AAM02090"/>
        <property type="gene ID" value="MK0877"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0877"/>
    <dbReference type="HOGENOM" id="CLU_1590901_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="18339" checksum="6D14C02C554D518C" modified="2002-06-01" version="1">MIRSDGTRAVASAGDEKFLKADEAEVRRELTPFEAYRKICKKLDASVVEPPEDVGHPVDPEVGYRVLELLESLWDSGFRRLGVLGTVGCGAPLHDIEVGNPVGWTSVRVSNPGSDLDLVAPHRDYLLEMSLSGFETVKRFADRVIMSPAAFTGFTVPRVEVRLPRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX11</accession>
    <name>Q8TX11_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02083">
        <property type="protein sequence ID" value="AAM02083"/>
        <property type="gene ID" value="MK0870"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0870"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.912"/>
    <dbReference type="HOGENOM" id="CLU_945306_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="294" mass="32140" checksum="5D01A8F36AF8C39E" modified="2002-06-01" version="1">MFGWIRRHRKSIVTAGIIFLAAMMVLSAIPSFMMGGQSAVAAAEGTVDASIVKDYQVSVVRYLAVPKPGVKTSDVKSAVSSAGGVENVDVRKVKIKGYTVFVVDALVPLTADPEDVKKRIEERLKDKVLDARGTVIKGSKFVFQASYDGKPDVNAIKRAIRRELGNRLMDGPKLKVRYNAVVGEVLCNVSDGKHTLIEERIERALHSTGARGCLVIEQLIARLGTAKLTLLGPGKEKVGDRTVNFGDRMAMVLVLPEHAKSKHLTLALWISNSGGKQRIYVVDRDILRGKYIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-02-26" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX16</accession>
    <name>Q8TX16_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FtsZ GTPase involved in cell division</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ftsZ_2</name>
        <name evidence="3" type="ordered locus">MK0863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02076">
        <property type="protein sequence ID" value="AAM02076"/>
        <property type="gene ID" value="MK0863"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0863"/>
    <dbReference type="HOGENOM" id="CLU_1163807_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26025" checksum="6BCE485163E0D048" modified="2002-06-01" version="1">MYYIGLGGFGCRFSTHASNAGLEVILCAGSRGDLLRVSEGEKIEVASGIGFSRDWRKASEELQEQAETIRETLERVGAEEPTVLAFGLGGAVGYALAQQILKEPPVPFIVLTTVPGESEDPQVRRNAAEQVKSVLGVSIKLPVLWVDNALGGYERVNRVLERTLLDLRDFLGVPVEELPNLAGNYALSPSISGEKVTGDVVKTSADEVRKLVKSREPEEHVVRRERRGPDPESLRELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX17</accession>
    <name>Q8TX17_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate uptake regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phoU</name>
        <name evidence="3" type="ordered locus">MK0862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0862"/>
    <dbReference type="EnsemblBacteria" id="AAM02075">
        <property type="protein sequence ID" value="AAM02075"/>
        <property type="gene ID" value="MK0862"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0862"/>
    <dbReference type="HOGENOM" id="CLU_989064_0_0_2"/>
    <dbReference type="OMA" id="DRCVNIV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="94"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="2">
        <location>
            <begin position="195"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="311" mass="35909" checksum="F3DD03066D43C0D9" modified="2002-06-01" version="1">MYPRGREATLAAVLHTILRDRPSTQKEIAEKVGVSRRYVTELLRPLIEEGAVRRTYTVDMSKLRRHFPELFEELGNFLYEDVEMHLEGVRPYFKRMTDLTLEQIETALIAVEENPEQAPKVIEMDEEVNRLDEEIRLYVRTLPVLHPCEEAGKVATLLLEISHCIERIGDYACNIAEVAETLGTLSDLQCWREVREAALEARSMVKTAYSIFLGRVKEEDLRHEAEKVYCAEDRVHKNIIRACEKAVEETKEFPEKADRLFGLSRITKDIERIADKAVDVVDFTRELVEGRPRDLTPEQEMRSTLPADGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX27</accession>
    <name>Q8TX27_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-15 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02064">
        <property type="protein sequence ID" value="AAM02064"/>
        <property type="gene ID" value="MK0851"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0851"/>
    <dbReference type="HOGENOM" id="CLU_616255_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="444" mass="48310" checksum="DCFE5233AAEC3711" modified="2002-06-01" version="1">MCFTLAAVVLISDSEIKWRIHDALYPMILGSTEAKDVMLLLHLGLLFLVLPYRSRIPLPKIDTWVTYALMAAILAVPPALQLLMIYEAGFNPGLGTCIVTLTERPETSSFYHCHAVKACLGALISAIFGEPKIEFHCGVPLSFVLPPWVPLPVLALLAIAYVVLAWIVPLRADRTEKAIVLSIAGSLLCLGCLDGGPLANPSVVGLSILLASRIRTVAPPIASLGVLGGLDAFRQVLFYLPAYHTLTYLWTAFLSSLLPSTVDRITRRSTLAVSLCLLGMLVPHLPVAEPLRSTLGLASEHDYYRVDVSNGDPAEVRAELSRIPGVEAVSIRVTDGRVTACVKTKRGVKLQVPKARVRKIRGYILELRVLYGSPRDLTEALKSTDGVVGVLGVKKMGSVYRVELGAEVDREVNLVPSMVKQLRRHGVVVSRIVTFTDVARCIWG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX28</accession>
    <name>SYM_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="1">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metG</name>
        <name type="ordered locus">MK0850</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX28"/>
    <dbReference type="SMR" id="Q8TX28"/>
    <dbReference type="STRING" id="190192.MK0850"/>
    <dbReference type="EnsemblBacteria" id="AAM02063">
        <property type="protein sequence ID" value="AAM02063"/>
        <property type="gene ID" value="MK0850"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0850"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.893"/>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="PTHR45765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="SSF57770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000139188" description="Methionine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="567"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="332"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <sequence length="668" mass="77387" checksum="FBC5734FF5FEF015" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX31</accession>
    <name>Q8TX31_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protease subunit, stomatin/prohibitin homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0847</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0847"/>
    <dbReference type="EnsemblBacteria" id="AAM02060">
        <property type="protein sequence ID" value="AAM02060"/>
        <property type="gene ID" value="MK0847"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0847"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.890"/>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="OMA" id="NAVLYFR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="PTHR10264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="245" mass="27440" checksum="09BBDD1D9F0B050A" modified="2002-06-01" version="1">MIIPLVVGGVLALLVLAASVRIVNQYERGVLLRLGRYIGTREPGLNFIVPFIDKMIKVDLRVVTQNIPAQEVITKDNVPIKVDAVIYYRVVDPVSAVLNVEDYEEAVFNLAQTTLRSVLGEVDLDDILAKREELSERIREIIDEKTEGWGIHVTGVEIRDVILPEEMRRAIARQAEAERDRRARVIQAEAEKQAAQDLRKASEVLGVNPGLLRTLQTLSEVSAEENVTIVIPVPIELLKLLKEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX41</accession>
    <name>Q8TX41_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02049">
        <property type="protein sequence ID" value="AAM02049"/>
        <property type="gene ID" value="MK0836"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0836"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.879"/>
    <dbReference type="HOGENOM" id="CLU_622044_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="440" mass="47572" checksum="BE5EA2C8F454B5A4" modified="2002-06-01" version="1">MRRLVLPTLLTMVFLAVPATADTTATTPTAEVSTSTQGTLQQVTQQTTQQEQTPGASGELLPQVAPKALGYIAVGAILCAIPNRGTQELGADLTQLGAEALTLAAAVDHGLPLKYPVAYQFNRTYEKLSSPMYWGEVGFNIVAPMLVNSMLYSMLQPLSEQLEVPPDVIKNYIVQTCKLMAKLQDRPVSPEDVIPVIDSAIQIINQSSNQSSQSKLTEDTKYEVAKTIANFLNGIRGSSGVLIASNVCKFALRYINVEPSNGIKLWIGSVLSPVVPDVSGTISDVRNITRDINNAMIGVRIATNPVETIRQITEDTGNSIIETFRTIVKNADKLGISEETANEILDDLKSMISDLTSTITDEIVRPYEPIVRCLGIALAINSLMDAYNSFNRLTQRLSKLALIARGLGVQVPTLTNPEGELKRALGVLQQGRVGPPPLPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX58</accession>
    <name>QUEE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="1">CDG synthase</shortName>
            <ecNumber evidence="1">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queE</name>
        <name type="ordered locus">MK0818</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="1">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX58"/>
    <dbReference type="SMR" id="Q8TX58"/>
    <dbReference type="EnsemblBacteria" id="AAM02031">
        <property type="protein sequence ID" value="AAM02031"/>
        <property type="gene ID" value="MK0818"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0818"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.860"/>
    <dbReference type="HOGENOM" id="CLU_066739_1_0_2"/>
    <dbReference type="OMA" id="RIIPQCH"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000416218" description="7-carboxy-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="28"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="252" mass="27940" checksum="BEA2C36BE9C4B32C" modified="2002-06-01" version="1">MGTGGRGRPTPGLNVYEVFLSLQGEGKFVGEPQAFVRFSGCNLRCAYCDEPASRSSRRRALIRRVSGEVELELPVPCGPEDVVEVLVELEDLEDTFGTVSLTGGEPLVQPWGALKELIERLRERGFRVLLETNASLPDRAPLIDELADVVSADVKLPSHGPNMDDFPDRCLRFLERISAEVYAKVVLVDEECYQHAESALKGLHRLGVEPIYLQPATGSEHDLEDLWELAGLVNADVRVLPQVHKLVDFIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX63</accession>
    <name>Q8TX63_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted membrane-associated Zn-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0813"/>
    <dbReference type="EnsemblBacteria" id="AAM02026">
        <property type="protein sequence ID" value="AAM02026"/>
        <property type="gene ID" value="MK0813"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0813"/>
    <dbReference type="HOGENOM" id="CLU_663266_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044838">
        <property type="entry name" value="EGY1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31412">
        <property type="entry name" value="PTHR31412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="414" mass="46680" checksum="FFA032616791F652" modified="2002-06-01" version="1">MRRQLPLEPVAAEKVEEEYELVTLLVCDNCDYFEFKPHDREVVYERKGKCPKCGGTLLVEGVFRTDADPSMFPSYKDVKSVERALESAGYEVKEFRVREDGSVVFHYERKGDAHRLARKLAEMKFTAEPEGKAGRLVVQRFEVPEQEPGWLALLGLSVPVFAYLGGWLAFGSTWAAVEFALAFSAIYFGKEIAKLWVAKVEGLRPRLPFFLAVPPFPGAFSSVIRSEVRPMLVESLCRVGVAGLVAGFLLSTAMFLLGSAFDHTPVRMLVWHNPWTLLLSRELGIVANPITLAGWAGLVITWLSALPVYPLEGGYILRYYYDTRTVKWFSVASAFIQGWLHWYHIAVATVIVLVKITAKLPSDRSFLDDDESKSWLPALLLVALFVLLISPAPFGLWPLEHSKVPHNLQWLFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB2</accession>
    <name>Q8TXB2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0762"/>
    <dbReference type="EnsemblBacteria" id="AAM01976">
        <property type="protein sequence ID" value="AAM01976"/>
        <property type="gene ID" value="MK0762"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0762"/>
    <dbReference type="HOGENOM" id="CLU_1118217_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018216">
        <property type="term" value="P:peptidyl-arginine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021172">
        <property type="entry name" value="UCP006607_RNA_methylase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PTHR11006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006607">
        <property type="entry name" value="RNAmts_UCP006607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="3">
        <location>
            <begin position="43"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="248" mass="27673" checksum="98B5CD935AF89FD9" modified="2002-06-01" version="1">MIKTIRTPVGEFKLLVDSYQLDLLRDVRRVFVFTSMVAEVAEDTFADLGAGTGPLSVVAAHAGAERVIAVEKNPKRARLLEKNLRKHVPHDVEWEVVVGDARDVDVNADVVACEMIDTLLLEEKFVPVINAVLERYEPTIVPQEVRIGANPIRRPPRTPRYRPGLPEDIEPLEVIRTDKPIPKKFEYETPEPGYAFFTWVEYEGTIAGGSDVFCPVLELPTPGDVLIGVRGAGLPSLRSYTPEHQDHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC2</accession>
    <name>Q8TXC2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylmuramyl tripeptide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">murE_1</name>
        <name evidence="3" type="ordered locus">MK0752</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0752"/>
    <dbReference type="EnsemblBacteria" id="AAM01966">
        <property type="protein sequence ID" value="AAM01966"/>
        <property type="gene ID" value="MK0752"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0752"/>
    <dbReference type="HOGENOM" id="CLU_022291_4_2_2"/>
    <dbReference type="OMA" id="IVIRHWI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="111"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="1">
        <location>
            <begin position="326"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="480" mass="53657" checksum="2AC4A9DD2EB43B68" modified="2002-06-01" version="1">MKLTVRELAENVEGEIRSGDPDSMIAGWFSTLGRAKEGDVVIRWWLDDKGAEIASERGVACLVTEHPRGKLIERCDELGIPLILTEVDRANEYALRVAREKCAPDATAICVTGTNGKSTTNHLLHHILDTAGFDAYCNTDFRSEKNTLIDPVVAMELREERPEDYLCVEVSEVQGLPTGRIMEDHAYRMAKALKCELAVVTNVGVDHTNLVSSLEEMVDAVYGVVRALRKGGIAVLNRDDRYVRRMTREGVETVWYGWDEGYSHIEERDGEEWIVLDEEPLIPVREFPLPVDHFLYNALAAVAAAGALGIDREDIAEGLRTYQPLKRRFERLCDDPLIYDDFCHNPDGVLATVRALRRLGRSKVIVVFAIRGSRGVDINRQIARALAEGAKDLQDEGIHVHVIATSSEGLVDDANVVRPEERRAFLEELERSGIDYEHYDRLEDALERALDLADDDTVILLGGAQGMEPAREVLRDMGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD1</accession>
    <name>Q8TXD1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histones H3/H4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hht1_2</name>
        <name evidence="1" type="ordered locus">MK0743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0743"/>
    <dbReference type="EnsemblBacteria" id="AAM01957">
        <property type="protein sequence ID" value="AAM01957"/>
        <property type="gene ID" value="MK0743"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0743"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.783"/>
    <dbReference type="HOGENOM" id="CLU_2420056_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="91" mass="9748" checksum="45F4D4367504A7EE" modified="2002-06-01" version="1">MDPDLPTNVLEEILKKSARSADGVLGVRMSAKKEYYRVIESLSLALAELSVLAARHAGRKTVKAEDVELAAATLDVISSIRDPRGRGGSRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF5</accession>
    <name>ACDA2_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Putative acetyl-CoA decarbonylase/synthase complex subunit alpha-like</fullName>
            <shortName>ACDS complex subunit alpha-like</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>ACDS complex carbon monoxide dehydrogenase subunit alpha-like</fullName>
            <shortName>ACDS CODH subunit alpha-like</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cdhA2</name>
        <name type="ordered locus">MK0719</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">This protein lacks several conserved Cys residues that bind [4Fe-4S] clusters in other CODHs. Therefore, it is not clear whether this protein is active. However, the protein would be able to bind a [Ni-4Fe-4S] cluster, which is the active site of CO oxidation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF5"/>
    <dbReference type="SMR" id="Q8TXF5"/>
    <dbReference type="STRING" id="190192.MK0719"/>
    <dbReference type="EnsemblBacteria" id="AAM01933">
        <property type="protein sequence ID" value="AAM01933"/>
        <property type="gene ID" value="MK0719"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0719"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.760"/>
    <dbReference type="HOGENOM" id="CLU_361186_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155079" description="Putative acetyl-CoA decarbonylase/synthase complex subunit alpha-like">
        <location>
            <begin position="1"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="632" mass="68559" checksum="722B7DCE1C510A08" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH9</accession>
    <name>Q8TXH9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01909">
        <property type="protein sequence ID" value="AAM01909"/>
        <property type="gene ID" value="MK0695"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0695"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.733"/>
    <dbReference type="HOGENOM" id="CLU_1418701_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="191" mass="20742" checksum="2B4E745B4D5EAFEB" modified="2002-06-01" version="1">MRVRSWFSATPSGQVTIEFAALLLIMLVLSAYFTYSVVGTFLGVSDVMALIEARYIANYVANAIAGTISWLPDWQSTANQIHLPEKIGNSEYYLKVRVKGEPGGPGEIVVILRLGGRFEKKVGVTASVPILVGWPSGGWPPRTPGVVLIDPRTVDDPVPTPVTEWPPGDEWWVSTSGKPFEFGLYVIPPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI2</accession>
    <name>Q8TXI2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted P-loop ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0692</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0692"/>
    <dbReference type="EnsemblBacteria" id="AAM01906">
        <property type="protein sequence ID" value="AAM01906"/>
        <property type="gene ID" value="MK0692"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0692"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.730"/>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="OMA" id="CLVSQRP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HAS-barrel" evidence="2">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DUF87" evidence="1">
        <location>
            <begin position="133"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="527" mass="59416" checksum="2EFBD18B8D3AA1EB" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-21" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI3</accession>
    <name>MRE11_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mre11</name>
        <name type="synonym">sbcD</name>
        <name type="ordered locus">MK0691</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXI3"/>
    <dbReference type="SMR" id="Q8TXI3"/>
    <dbReference type="STRING" id="190192.MK0691"/>
    <dbReference type="PRIDE" id="Q8TXI3"/>
    <dbReference type="EnsemblBacteria" id="AAM01905">
        <property type="protein sequence ID" value="AAM01905"/>
        <property type="gene ID" value="MK0691"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0691"/>
    <dbReference type="HOGENOM" id="CLU_606374_0_0_2"/>
    <dbReference type="OMA" id="PFAHADW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138690" description="DNA double-strand break repair protein Mre11">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="374"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="451" mass="50627" checksum="52710611BB87D75E" modified="2002-06-01" version="1">MRMAHVADVHLGHALMNLRSREEAVMETFERLMEEVRECSVDVLVIAGDLFEHARPKTEALYLAVEKLSELKEDGVEIVATAGNHEIRRRKGAVSPISVLERMGLVRHLYYSERRPERHRYTATFDGVRVTFHGLQYLPKNSFVERAKVIRAKYRPDPEADVNVAIFHQALPGTIPDESEIVEPAYFPEGHDYYAMGHVHVPSREEKIHGSPAPYPGSPEPLTFLEVKDERGAHKRRGFFLVEFDRGGLVEYEFVEVEWSRELSVVEVSGERWEEELRRRVRRGQIVKVVAKDTGASPEEVEKVAIEAGADRCVVELRERRREVEEGDETEGPLDLEGIIREGVKRARAATLTRVDVPDDVVVEVALEILRGVREDNPPDLGDVEGIVAGEPPSEGSEESSEEPEESDGEEVGLEVEEVKVESRGTSSEGMSRAGSKLGSSGRPSLDRWIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN2</accession>
    <name>Q8TXN2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing V4R domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01845">
        <property type="protein sequence ID" value="AAM01845"/>
        <property type="gene ID" value="MK0630"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0630"/>
    <dbReference type="HOGENOM" id="CLU_1881091_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="82"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="135" mass="14621" checksum="24C296152461603E" modified="2002-06-01" version="1">MDLTEETLLRGAEATAEAFAPYELLTYDRLTRIALIEVARETGTAALFEAGRRLVRILGGDDLESVLCAFAEVFGAEVEVEGDTVTVRKCPECAGLRGIDGPVCHLTRGFITEAYRLEIGRPVTVAETRCRAAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN8</accession>
    <name>Q8TXN8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted cysteine protease of the transglutaminase-like supefamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01838">
        <property type="protein sequence ID" value="AAM01838"/>
        <property type="gene ID" value="MK0623"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0623"/>
    <dbReference type="HOGENOM" id="CLU_1280806_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="127"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="215" mass="24614" checksum="BF3C75D320889875" modified="2002-06-01" version="1">MALLLTLILALGFTSLPLNAPPHLSADVPDTYPWVGVYPPDVEVTYVNNWDPNLNPEHIEDWKCIAREILGDPKSWWEQYTAGTPLDELVYEAAKKIEYWVSPYDDVDPHGIKYRYYYNTRYGAWKTLQLGRGNCCDHAHVMVALCRASGIAIRYRHEVTRFPCWGTCDHVCVEVGLPENVVDGKSQDVKWVRMDPTNPNSQCVRLKATYPNLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR0</accession>
    <name>Q8TXR0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0600"/>
    <dbReference type="EnsemblBacteria" id="AAM01815">
        <property type="protein sequence ID" value="AAM01815"/>
        <property type="gene ID" value="MK0600"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0600"/>
    <dbReference type="HOGENOM" id="CLU_140136_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="17221" checksum="87D1C3DFC2860675" modified="2002-06-01" version="1">MIPVTVVYHNVHSPRKVEEMARTVAGFGAKRFVISRALGSAAQEGVPKAQRICLEAGVELLFFQDLEEALKALSPDVTYMAEDAEHGGAKPLDFDAVVEEIEQEREVCFVFGSARPGLTKQELELGDEAVYVGTERNVGEVGAVAILLHELRKRLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR7</accession>
    <name>Q8TXR7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0593</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01808">
        <property type="protein sequence ID" value="AAM01808"/>
        <property type="gene ID" value="MK0593"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0593"/>
    <dbReference type="HOGENOM" id="CLU_1718225_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="152" mass="16981" checksum="C467C04E4C0F34C7" modified="2002-06-01" version="1">MCYEIHTQNCTVESVTVELLKRTDQGWVVVDRRTLSATSDVVTFRVSEEGQYKVRITAQCTHGVTMTAETEPVTVQFLPKVEISGFKVVETDYGPDWVELRITADVHVERAEKVSGLVVVEDAKGNVLGKFPVEISDHMDVKVRLEGLENVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS8</accession>
    <name>Q8TXS8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK0582</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01797">
        <property type="protein sequence ID" value="AAM01797"/>
        <property type="gene ID" value="MK0582"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0582"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.617"/>
    <dbReference type="HOGENOM" id="CLU_027639_1_0_2"/>
    <dbReference type="OMA" id="PCYNEKL"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005980">
        <property type="entry name" value="Nase_CF_NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="PTHR43787:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="12"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="282" mass="31588" checksum="5FA8A54BEC6D93A6" modified="2002-06-01" version="1">MRGPCPNDHPCYVGEHGKFVKVHLPVGGRCNIHCRFCESGLEHEGVRVDYPGRAVRTITGDEARTALKRVKEHCGRVDVVGIAGPGDPLANWEDVKETFDVVAETVPEAKRCLSTNGVWLPDLIDEVTELVHSVTITVNALDPEIAADIYDRALTPEGEVLTGKEAARWIVERQEGAMDALKKERYILKKVNFVLVPGVNEDEVERVAERAADAGFHAMNVIPLIPGGDMKDHRPPTCRELSRARDRAEKYITVMRRCMQCRADVIHCRGRPRLIWELLEED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW1</accession>
    <name>AMZA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaemetzincin</fullName>
            <ecNumber evidence="1 2">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">amzA</name>
        <name type="ordered locus">MK0548</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="PLoS ONE" volume="7" first="E43863" last="E43863">
            <title>Crystal structures of archaemetzincin reveal a moldable substrate-binding site.</title>
            <authorList>
                <person name="Graef C."/>
                <person name="Schacherl M."/>
                <person name="Waltersperger S."/>
                <person name="Baumann U."/>
            </authorList>
            <dbReference type="PubMed" id="22937112"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0043863"/>
        </citation>
        <scope>MUTAGENESIS OF CYS-163</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="Proteins" volume="78" first="2720" last="2723">
            <title>Crystal structure of archaemetzincin AmzA from Methanopyrus kandleri at 1.5 A resolution.</title>
            <authorList>
                <person name="Waltersperger S."/>
                <person name="Widmer C."/>
                <person name="Wang M."/>
                <person name="Baumann U."/>
            </authorList>
            <dbReference type="PubMed" id="20597090"/>
            <dbReference type="DOI" id="10.1002/prot.22777"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Probable zinc metalloprotease whose natural substrate is unknown. Does not show endo- or exopeptidase activity against resorufin labeled casein, p-nitroanilide (pNA), amidomethylcoumarin (AMC) (one to three amino acids in length), and hippuryl-aminoacid substrates.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 2 Zn(2+) ions per subunit. One is catalytic, whereas the other seems to have a structural role.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1 2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="2X7M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A=1-175"/>
    </dbReference>
    <dbReference type="PDBsum" id="2X7M"/>
    <dbReference type="AlphaFoldDB" id="Q8TXW1"/>
    <dbReference type="SMR" id="Q8TXW1"/>
    <dbReference type="STRING" id="190192.MK0548"/>
    <dbReference type="EnsemblBacteria" id="AAM01763">
        <property type="protein sequence ID" value="AAM01763"/>
        <property type="gene ID" value="MK0548"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0548"/>
    <dbReference type="HOGENOM" id="CLU_108521_2_0_2"/>
    <dbReference type="OMA" id="RSVYDCD"/>
    <dbReference type="EvolutionaryTrace" id="Q8TXW1"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01842">
        <property type="entry name" value="Archaemetzincin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012091">
        <property type="entry name" value="Pept_M54_archaemetzncn_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="PTHR15910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005785">
        <property type="entry name" value="Zn-prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159627" description="Archaemetzincin">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Unstable." evidence="3">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="34"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="56"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="71"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="95"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="149"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20597090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22937112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2X7M"/>
        </source>
    </evidence>
    <sequence length="175" mass="19827" checksum="95E277E4DE0604BF" modified="2002-06-01" version="1">MKLCLVAFDGRIPMLSSIVDRFEEHVSEYLGEVKVKKKRAKLPEHAYSKVRGQYLARALLDTLRGMKGEYDRVLGLTSEDLYAPGLNFVFGQARCPGREAVVSVARLLDPDPELYLERVVKELTHELGHTFGLGHCPDRNCVMSFSSSLLEVDRKSPNFCRRCTELLQRNLKRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW6</accession>
    <name>Q8TXW6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein, homolog of gamma-carboxymuconolactone decarboxylase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01758">
        <property type="protein sequence ID" value="AAM01758"/>
        <property type="gene ID" value="MK0543"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0543"/>
    <dbReference type="HOGENOM" id="CLU_2127837_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="113" mass="12439" checksum="11B1BF4C9E2410CB" modified="2002-06-01" version="1">MSRKPGEPAKEEDEKGLIAVKYAEEMLGDEYAEVVEELHRIIWKESPLDDKTKHLIALALAAAAGDERRVGLIAETAKNVAEVTDEEIAATLALVAWVEGVSKVTRTWGVLRQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-01-10" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW8</accession>
    <name>AGOG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-glycosylase/DNA lyase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">8-oxoguanine DNA glycosylase</fullName>
            <ecNumber evidence="1">3.2.2.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AGOG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="1">AP lyase</shortName>
            <ecNumber evidence="1">4.2.99.18</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0541</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text>Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EC" id="4.2.99.18" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXW8"/>
    <dbReference type="SMR" id="Q8TXW8"/>
    <dbReference type="STRING" id="190192.MK0541"/>
    <dbReference type="EnsemblBacteria" id="AAM01756">
        <property type="protein sequence ID" value="AAM01756"/>
        <property type="gene ID" value="MK0541"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0541"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.576"/>
    <dbReference type="HOGENOM" id="CLU_085935_0_0_2"/>
    <dbReference type="OMA" id="VKMFGYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000702">
        <property type="term" value="F:oxidized base lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01168">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016544">
        <property type="entry name" value="AGOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015254">
        <property type="entry name" value="AGOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09171">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008955">
        <property type="entry name" value="AGOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185110" description="N-glycosylase/DNA lyase">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Helix-hairpin-helix">
        <location>
            <begin position="119"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DNA" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>8-oxoguanine</name>
            <dbReference type="ChEBI" id="CHEBI:52617"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01168"/>
        </source>
    </evidence>
    <sequence length="242" mass="28614" checksum="25FF075E3383719E" modified="2002-06-01" version="1">MTVPTFLRKVTLREICVIEEYVDVQYRAIEALMDTLPSTDLVRLTVANALVSYQLSSSGEDWWREFSSYFRERRPRDIVREYARFLPRSRGNRRLIRQKLRRLHRAKAFLEELSWQDAKSYYRDMNRLRLDLARVLNADPESKTIVFTVKMFGYALRAITGRFRPYPFEIPIPVDARIERITRRITNDDPQLYWDSIARRTGIPPLHLDSILWVGTSRDPEVKRLLAKVLPKLIGELEMLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY03</accession>
    <name>Q8TY03_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amidase related to nicotinamidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0504"/>
    <dbReference type="EnsemblBacteria" id="AAM01719">
        <property type="protein sequence ID" value="AAM01719"/>
        <property type="gene ID" value="MK0504"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0504"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.535"/>
    <dbReference type="HOGENOM" id="CLU_068979_8_4_2"/>
    <dbReference type="OMA" id="WHKSNAL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008908">
        <property type="term" value="F:isochorismatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016291">
        <property type="entry name" value="Isochorismatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01398">
        <property type="entry name" value="ISCHRISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="20231" checksum="807563CD2EC335FD" modified="2002-06-01" version="1">MRALLIVDMIRDFVEEGAPLEVPKARRLVPRIARLADEFRERGDLVVHVWDEHYPDDPEFKVWGEHAVAGTEGAEPVEELKPEDGDLVVRKRKYSGFYGTSLDYDLRSRNVKEIYLTGVCTDICVLFTCADALMRGYRVYVVRDCVASLEEESHRFALKHMEKLGAEVVDSEEILGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY34</accession>
    <name>Q8TY34_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ehaF</name>
        <name evidence="1" type="ordered locus">MK0472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0472"/>
    <dbReference type="EnsemblBacteria" id="AAM01687">
        <property type="protein sequence ID" value="AAM01687"/>
        <property type="gene ID" value="MK0472"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0472"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.502"/>
    <dbReference type="HOGENOM" id="CLU_1639963_0_0_2"/>
    <dbReference type="OMA" id="YTRGMDT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011313">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09879">
        <property type="entry name" value="DUF2106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019373">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="169" mass="18928" checksum="DC19DD7A88B779B2" modified="2002-06-01" version="1">MSVTAKFSRALNAIRRPESMVSVYCLLLAVLALLGLHCGHSYHREQLYPRPAPQVQMKAGDPLAPYDRGGVPLESPGVTISQYPQFEPVRGWVTSYLTPFTRWLAHNSRHCGTTIVSHPGGILDEILYYTRGLDTVLESSIMFVAFVTFSWIVRTKTIGEEEMEREGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY56</accession>
    <name>Q8TY56_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01665">
        <property type="protein sequence ID" value="AAM01665"/>
        <property type="gene ID" value="MK0450"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0450"/>
    <dbReference type="HOGENOM" id="CLU_1418700_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="191" mass="21356" checksum="C96FE9F0BF7259DD" modified="2002-06-01" version="1">MSLVKSAKNDVVIVVGEDIDEFNKGVLSSVCKETGKVILFGKDYLNKFSIDEKLETSEESLPIKRCDVAIVDEDTEVDGKLLLDVPADEYTINEVLSLVKEQFKEVCDYERGVEADVLLVIDSDRGRLRIYANGDEDEVEDKARELCMKVAERVVEERRSAHIDIRTYGFALVSVALAIVVVTLILRTSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY75</accession>
    <name>Q8TY75_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="4">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name evidence="5" type="ordered locus">MK0430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0430"/>
    <dbReference type="EnsemblBacteria" id="AAM01645">
        <property type="protein sequence ID" value="AAM01645"/>
        <property type="gene ID" value="MK0430"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0430"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.459"/>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="OMA" id="MWGGGMM"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="52"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="245" mass="25869" checksum="8943D49DF1315885" modified="2002-06-01" version="1">MEREITPIVLREGYEFINDCSESDVIVVGAGPAGLTCAYELAKSDVDVTIVERKLYVGGGMTGGGMLFPAGVIMEETAEVLEEVGVELRPAEAGLLAFNPVEAAIKLANAALEAGARILVGIEVEDVIERRGRVCGVVVNWTAVKAANMHVDPLALEAEYTVDATGHEAAVCKLAGIEVKGEGPMWAERGEELVVKHTQEVKPGLFVAGMAASAVKGAYRMGPIFGGMLESGKKAAEEILERLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY96</accession>
    <name>Q8TY96_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted intracellular protease/amidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0409</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0409"/>
    <dbReference type="EnsemblBacteria" id="AAM01624">
        <property type="protein sequence ID" value="AAM01624"/>
        <property type="gene ID" value="MK0409"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0409"/>
    <dbReference type="HOGENOM" id="CLU_000445_44_2_2"/>
    <dbReference type="OMA" id="TSYPAMK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1_PfpI" evidence="1">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="179" mass="18658" checksum="9CAFC29DAC7F6021" modified="2002-06-01" version="1">MIAVLVDDGFEELELGAVVSVLSRGGLDWDLVGVEERAEGMGGMEVGVDSTVWDVEGDDYEGVVVLGGSAPTTLIGYRHCLDLVRSVESDGGMVVGLSSGALVLAEAGVLRGRKATTYPGFEAELKVNGAEPVPKGVVRDGNVVTSRGPAFAIDACLEVVRELCGDHMANSVARQLILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA8</accession>
    <name>Q8TYA8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(2R)-phospho-3-sulfolactate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">comA</name>
        <name evidence="2" type="ordered locus">MK0394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0394"/>
    <dbReference type="EnsemblBacteria" id="AAM01609">
        <property type="protein sequence ID" value="AAM01609"/>
        <property type="gene ID" value="MK0394"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0394"/>
    <dbReference type="HOGENOM" id="CLU_062679_2_0_2"/>
    <dbReference type="OMA" id="MFEASNA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043817">
        <property type="term" value="F:phosphosulfolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022370">
        <property type="entry name" value="Arch_ComA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="SSF102110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03849">
        <property type="entry name" value="arch_ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="256" mass="28102" checksum="5DE76A2A05EF084E" modified="2002-06-01" version="1">MKAFEKSVKIPKVDGATVMLDKGLTPEFVESFLKVAGEYVTAVKLGWGTARLIDKEIVARKVEMYVDAGLDVFPGGTLAEIAIAQGNFEGYLNELDELGFNAIEISDGMIPMSIEKKCELIERACEQGFTVYAEEGKKRDEEYSVLSPSDIVGRMNKCVEAGAEYVIVEARESGKHGPMGAEKRERVRVLSEIVKGVGIQRVMFEAPEKEQQFELIVKFGPEVNIANVPPEEVIPLATLRAGLRAETMGRVALDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB2</accession>
    <name>Q8TYB2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted HD-superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0390"/>
    <dbReference type="EnsemblBacteria" id="AAM01605">
        <property type="protein sequence ID" value="AAM01605"/>
        <property type="gene ID" value="MK0390"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0390"/>
    <dbReference type="HOGENOM" id="CLU_1109506_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="62"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="27566" checksum="5DF6D255F0F40BCE" modified="2002-06-01" version="1">MRTEELKLKLKELVESIEDEGLRELVMKVLEEGFAHEEVPDPEPVEEAPASRRQHHSYPGGLLEHTVATTKLALAMAEVFEEIYGLEVDRDLVIAAAILHDLGKATSYERREERYKISDFGRRLDHLTLIAAELYARGAPVELIHAVAAHHGRGSPVPPNTPEALIVHLADRSDAEFATEVIKAARNVVRARLRELDVEPTDELVEEVLRRVGPSEIFLTRVREGRDAVRQLVAETLEEIEEGSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG4</accession>
    <name>Q8TYG4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gcn3</name>
        <name evidence="3" type="ordered locus">MK0335</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0335"/>
    <dbReference type="EnsemblBacteria" id="AAM01550">
        <property type="protein sequence ID" value="AAM01550"/>
        <property type="gene ID" value="MK0335"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0335"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.356"/>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="234"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="328" mass="36146" checksum="DBD10E44569290B1" modified="2002-06-01" version="1">MKPVSAFKTLTLRVYWDEARFEVLDQTELPDREVWRECTSYEDAAEAIENMRVRGAPLIGAVAALGAWVAYERDEDYGEAIERLRNTRPTARNLFWALERMEEAKNPRKEAERILREDVEVNRELGDHGAELLPDECTVLTHCNAGALACVDWGTALGVVRSAVFNMSKEVEVIATETRPVQQGARLTCWELSRDGIPVTLIADTAVGYVLASGKVDAIIVGADRIALDGSVANKIGTYQIAVLADRHDVPFYVAAPTSTIDPETETGEDIPIEHRSEDEVKNVRGVRVAPEDVPALNPAFDVTPPELIDAIITEKGVVEPQEVADLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG5</accession>
    <name>CBID_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiD</name>
        <name type="ordered locus">MK0334</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYG5"/>
    <dbReference type="SMR" id="Q8TYG5"/>
    <dbReference type="STRING" id="190192.MK0334"/>
    <dbReference type="EnsemblBacteria" id="AAM01549">
        <property type="protein sequence ID" value="AAM01549"/>
        <property type="gene ID" value="MK0334"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0334"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.355"/>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="OMA" id="YHGKLIK"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141693" description="Cobalt-precorrin-5B C(1)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <sequence length="409" mass="44576" checksum="03B34684DA0F8255" modified="2002-06-01" version="1">MNDLLDPIRGIPVDLELVRRELDVDLPTARYLVRTGLITTDGARVLRRGPTTGTCATAAAKAAAIRLLEGRTVRTVRVRLPVGTVIGVRISRVGGDPSEARVRKPGSDDHVDVTTGVTIAARVEETGSEGVEIRAGRGVGETPSGKPAISEAVREQIVDNLRYLVDSYGVGLRVTIEVPDGEEIARKTLAHRHGIEGGISILGTKGLVDPNSEEAIEGSIRSDLRYVERVPCLVTGYRTMDRARRLGIPSRDIVNCHGRYDLALEAVKTGVPADGEVKRFDAVLIFGMPGKLLKLAAGAYNTHAKVADARRESLVTRLVEIGRPDLAVEAARHEGLISEFLRSLDPDVRRELFERVCELVEERVSSDHDLECGCALYFRADDSEEVVEGEGWKRLVRGYDDDLIGRPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG7</accession>
    <name>Q8TYG7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0332"/>
    <dbReference type="EnsemblBacteria" id="AAM01547">
        <property type="protein sequence ID" value="AAM01547"/>
        <property type="gene ID" value="MK0332"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0332"/>
    <dbReference type="HOGENOM" id="CLU_132457_1_0_2"/>
    <dbReference type="OMA" id="ETCFGAY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012025">
        <property type="entry name" value="UCP005642_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09875">
        <property type="entry name" value="DUF2102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005642">
        <property type="entry name" value="UCP005642"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03272">
        <property type="entry name" value="methan_mark_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="141" mass="15678" checksum="CEDC612D8CD66AFF" modified="2002-06-01" version="1">MPLNVSRYFALAPGTGVSPKELVQWLLENSPPEVTIKETCFGAIADGPEDELRKLAERAREEFGYAVFSKVRGYPAGDPRVCRRTRGGGPRPGFPQLQREVELLDLIAEGLRALDKGEEVELEEREPVSADTIRKLAEELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ6</accession>
    <name>Q8TYJ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing PIN domain, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01518">
        <property type="protein sequence ID" value="AAM01518"/>
        <property type="gene ID" value="MK0301"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0301"/>
    <dbReference type="HOGENOM" id="CLU_1933263_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="130" mass="14461" checksum="4F0F046949FB2FAF" modified="2002-06-01" version="1">MESVYTFTLSLFGCLDVTSVVGPRLMKDYGKNLAGESFLNRCWNPGCSSTARRGVQVGGSGRRARGVVSSDPPQGRSRGRRARSRLPTNQNGIDHPRHFSEVARELRGESLALKRRTPFDFEFDVLECRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK6</accession>
    <name>Q8TYK6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01507">
        <property type="protein sequence ID" value="AAM01507"/>
        <property type="gene ID" value="MK0290"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0290"/>
    <dbReference type="HOGENOM" id="CLU_2695792_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="73" mass="7907" checksum="1F34344C6BE9A286" modified="2002-06-01" version="1">MMNVGAREHHIDGNTVKFFGIERWDGHAPEVPVPVVAAFTAPGGSVDNVEEVGKEVLSKGQRYPRSTARALGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK8</accession>
    <name>Q8TYK8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flavodoxins</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01505">
        <property type="protein sequence ID" value="AAM01505"/>
        <property type="gene ID" value="MK0288"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0288"/>
    <dbReference type="HOGENOM" id="CLU_1954680_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="128" mass="14384" checksum="79EA3C885EE26D94" modified="2002-06-01" version="1">MIAQVIASTTDASLVEILSGNERLDAELAFLGTPVHAFRPAEPVRHFVRNNDWSDVKVALFCTYSLHPGKTIRWMREKIERAGGEVIGELTVKGEHPFLPLIARGHPDERDVQKARKFAKEVLRKARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP1</accession>
    <name>Q8TYP1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphoenolpyruvate synthase</fullName>
            <shortName evidence="7">PEP synthase</shortName>
            <ecNumber evidence="7">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ppsA</name>
        <name evidence="11" type="ordered locus">MK0252</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 7">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + pyruvate = AMP + 2 H(+) + phosphate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 7">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0252"/>
    <dbReference type="EnsemblBacteria" id="AAM01469">
        <property type="protein sequence ID" value="AAM01469"/>
        <property type="gene ID" value="MK0252"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0252"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.255"/>
    <dbReference type="HOGENOM" id="CLU_007308_6_2_2"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PTHR43030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000854">
        <property type="entry name" value="PEP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPDK_N" evidence="9">
        <location>
            <begin position="17"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="8">
        <location>
            <begin position="367"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="10">
        <location>
            <begin position="469"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="770" mass="85980" checksum="25A0B3D98ACA80D9" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP2</accession>
    <name>RL10E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10e</name>
        <name type="ordered locus">MK0251</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYP2"/>
    <dbReference type="SMR" id="Q8TYP2"/>
    <dbReference type="STRING" id="190192.MK0251"/>
    <dbReference type="PRIDE" id="Q8TYP2"/>
    <dbReference type="EnsemblBacteria" id="AAM01468">
        <property type="protein sequence ID" value="AAM01468"/>
        <property type="gene ID" value="MK0251"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0251"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.254"/>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="OMA" id="HHVIREN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_L10e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_L10e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_L10e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="PTHR11726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000147136" description="50S ribosomal protein L10e">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <sequence length="173" mass="19647" checksum="ED1CE85179015884" modified="2002-06-01" version="1">MVNRPARIYREWKGPAYTRREYIKGVPDPKIQQFDMGNPAGDFDVEVSLVARERAQVTHNALEAARVAANRYLTKTVGRQNYHLKIRVYPHHVLRENPLATGAGADRVQEGMRLAFGKPIGTAARVREGQRVVTVRIDPENFEHAKEALRRAGMKFPFPFTIVVDKGEELVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ4</accession>
    <name>Q8TYQ4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">gatA</name>
        <name evidence="9" type="ordered locus">MK0238</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0238"/>
    <dbReference type="EnsemblBacteria" id="AAM01455">
        <property type="protein sequence ID" value="AAM01455"/>
        <property type="gene ID" value="MK0238"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0238"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.239"/>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="49"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="455" mass="48829" checksum="A0BE405605EAA7B7" modified="2002-06-01" version="1">MLREAVKDIESGHVDPSERIHEYLEKIDRENPDINALIYINGGAPEEVGSEGPLAGVAIAVKANINVEGMPCDCASKTLEGYRAPFDATVVRRIKEAGAAVIGIANMDEFAAGSSGETSCHGPTDNPRCPGRIPGGSSSGSAAAVAAGLCDAALGSDTGGSIRNPASHCGVVGFKPTYGLVPRQGLIDLAMSLDQIGPITRTVEDAALLLEVIAGPGEDVEGTVRNAEVPRFSELLDPEEVEGMRIGLVREFLEVSEPEIAEVAKEAARALERAGATVEEVSLGRKLVDVALPTYYVINYVEFFSATRRFDGRRYGRRIEHVCGKEVLRRIVAGAAISRQEVRGQYYERALRARTWIRRRLLEALEGYDALLGPTVPKPPHRIGEELSVREMYGYDVLTVIPNLAGCPAGSVPFDTINVDGDRVPCGVQVIARPWEDLTALNVMAALERAASFEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ8</accession>
    <name>Q8TYQ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted decarboxylase related 3-polyprenyl-4-hydroxybenzoate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiD</name>
        <name evidence="1" type="ordered locus">MK0234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0234"/>
    <dbReference type="EnsemblBacteria" id="AAM01451">
        <property type="protein sequence ID" value="AAM01451"/>
        <property type="gene ID" value="MK0234"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0234"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.235"/>
    <dbReference type="HOGENOM" id="CLU_023348_5_1_2"/>
    <dbReference type="OMA" id="TEGGCCW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="48147" checksum="98561B849C6FDAFF" modified="2002-06-01" version="1">MRDFLRCVEDDLVVVKEELSPEYEVPAVLQELDRPVVFEGVEGYDIPLVGNLCCSREYLVRALGAESWDDVLRKLAEAMDSPKEPRKERSPSFLEAERGPEFLKEFPMCRFYRTDGGPYLTASLVVAVEPEEGIPNASVHRMMYLGDGKFAVRVVPRHLYRYYEKADHDLPVAVCLGVDPRTMFACCARVPYEVSELDVAAAFWEDLRVYEVDYGIPVPAESEIVMVGRLTPERAPEGPFVDVTRTLDERRREPVFEVEKVYTREDPYHPIILPGGEEHRIMMGGPAEATVLAHVSRVSEVVKVRLTPGGGRWLHAVVSIRKRTEGEAVNAGLAALAAHPSLKHVVVVDEDVDPDDPEQVEYALATRFQADRDLHVVRGLGSTLDPSAEEGIMAKAVFDATAPVEKRERFEVVEVPVSDRVRRILDELP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR8</accession>
    <name>Y224_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein MK0224</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0224</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYR8"/>
    <dbReference type="SMR" id="Q8TYR8"/>
    <dbReference type="STRING" id="190192.MK0224"/>
    <dbReference type="EnsemblBacteria" id="AAM01441">
        <property type="protein sequence ID" value="AAM01441"/>
        <property type="gene ID" value="MK0224"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0224"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.225"/>
    <dbReference type="HOGENOM" id="CLU_053134_0_0_2"/>
    <dbReference type="OMA" id="TRWVAED"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="entry name" value="PTHR38811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="SSF52733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00303">
        <property type="entry name" value="TIGR00303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151052" description="UPF0284 protein MK0224">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <sequence length="378" mass="40330" checksum="26C0EB10DBCF6ADB" modified="2002-06-01" version="1">MPNANQASAGVGALKEVLPGIRALGDLEAAEKVVKRCSGGSTVMVCVIGSTEISRVPGISAAGKTPESTFHTPAGDVELIYYDRIINAEEVPQNPVGAPSPAVITKAVVNLASIPFLTVDAGAAVKPACPYIDLGGEVARDFREGPALSEETYDRLLEFGKTLGKELTRDVDFLTVGESVPGGTTTAMAVMTALGYETSEKFASSSHDSPHDIKERVVKEGLEAQGVEPGDLDAHEAIRRFGDPMMPAVVGIIYGSRTPVLLAGGTQMAPILAYLAEEGQLDPERVFVGTTKYVVEDEDSDIESLFRQVGDYVLFSADPGFSESKFRGFRLYEEGYVKEGVGAGGAQVAAALKTKGEITPKDVLRECERVYERWMDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS4</accession>
    <name>Q8TYS4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase involved in chromosome partitioning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0218"/>
    <dbReference type="EnsemblBacteria" id="AAM01435">
        <property type="protein sequence ID" value="AAM01435"/>
        <property type="gene ID" value="MK0218"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0218"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.220"/>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="269" mass="28479" checksum="1702FFB07340BCF8" modified="2002-06-01" version="1">MAEAELPESEACRVVCMASGKGGTGKTTVTANLGTALAELGAETYILDADIAMANLGLILRMEDAPVTLHDVLAGEADIEEAIYEGPHGVKVIPAGISLEGIRKANPDRLRDVVEHIIDRADFLLIDAPAGLGRDAITALSASTESLLVVNPEIASITDALKVKAVAERVDTQITGAVVNRVTKDKTELTKEEVEKILETPVMVEVPEDPEVRRAAAFGEPVVVRSPKSAAAQAFKKLAAELVGIEYEVPVPDKEGVLSKVIKGLFGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ2</accession>
    <name>Q8TYZ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01366">
        <property type="protein sequence ID" value="AAM01366"/>
        <property type="gene ID" value="MK0149"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0149"/>
    <dbReference type="HOGENOM" id="CLU_1275323_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="216" mass="23777" checksum="C12A951839EF9822" modified="2002-06-01" version="1">MNPATAELWTYLLVLAFSYGTAYLIVACEGPRYLLASGVVLLFINVLLWIHIFDTKARNGSPPTGAEIVRVRRALKLSFAILGLQFTGIASIPGLLMAFTALRRKVFYFMGITEFKALVYGTLIALVTLPCVIALRKRHYHWVLMGMPIDTVVSVRNTFRRVCAIDAVLPIPIVVAYVLLAKGGLGGEDIFTAAAVAGASSLFNRAKVRYFFDEGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ29</accession>
    <name>Q8TZ29_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01327">
        <property type="protein sequence ID" value="AAM01327"/>
        <property type="gene ID" value="MK0110"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0110"/>
    <dbReference type="HOGENOM" id="CLU_1140569_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="28446" checksum="48B1A9BCEE627E4E" modified="2002-06-01" version="1">MIRRLVERYLRHVTKKRLTRRPTTALIYTCLLGMKAIARAFSEETISSISPEPLWTVESVSLVRIGTYPGDVLCSELVSFLTREFEEEERVEDVLPEVEVEAEFLARVRRFNDLLYEDIELPFEIEGTGEEEPDLDLRLIEVLLALDEIPQSEQGRYAKPFILTCFETLSDYLDVLGLRWSDVDTVRRKVRIKGRVHRISRALAYELRRIPREGEEVFPISSVDVMRWERAVSKEVGRRLRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ32</accession>
    <name>Q8TZ32_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted diverged member of adenylate cyclase 3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01324">
        <property type="protein sequence ID" value="AAM01324"/>
        <property type="gene ID" value="MK0107"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0107"/>
    <dbReference type="HOGENOM" id="CLU_682593_0_0_2"/>
    <dbReference type="OMA" id="EGYREGG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="452" mass="50274" checksum="02A35C4EDFF5DBF8" modified="2002-06-01" version="1">MIHPSRLFVKLSASPRVDGEIVACGTKYDPITEEEREIVETLNSMYMLLTRFEGYPLPAPQVISNELGEVAEELRREGFGLTKIEQFKYDDPEVALVSKIVLDETRYVYCTYRNENDLQSLAHECQCTVLECGGGEATVEAPSKFHALRFAVQVPLRLHGSAAIGLTQAAATERFAKAERHADSEFAAFSKIGAEWIIHRGGDPLPPPRRTNTAPDRILYLDIVGSSELVRERGRRYLEGIMSRVIDVINEEEGVVLDHRRGGDDVIARFPTKSRALRAAIKIVGRLTEDDVKIKIGIGDSRGRAAENAVTVRERVDYDCSYIAFRFGPYLVAYVEPPDYAVRIFGRIPSESVRAVGASAIVGSLTALHPYLALPFFVYFPIAAAHRNRDSASVAVVWFLIWVLVTLSAAWFGLYVREHYLPRPLVLEMNALFSQLMNLAKTMAARAGAGTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ39</accession>
    <name>Q8TZ39_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-36 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01317">
        <property type="protein sequence ID" value="AAM01317"/>
        <property type="gene ID" value="MK0100"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0100"/>
    <dbReference type="HOGENOM" id="CLU_701340_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="393" mass="43172" checksum="4C27D51659C2C374" modified="2002-06-01" version="1">MRAAVTMIAFVVLMSPVCAEVHILTWAGEGADSTCVARTKEIVDWWDTYRAIGPDTTIVWHYTTRVDRWRTVLETGTDPLTGARISVVYVPGGWHPERYWDFDWVRALYHAQIDLGIGYVGICAGAYLHAGNTTYSTSGPGHDDVLQDGVTVIDGMNGPNRICRVLILQDNPLTPEWTWGNVFTYKYWNGPGFGPEPGLEFNSSYKCWVKITNIEGREVMIKIWPVGEYVDTCKGWAIVAGQYFVKEGDKWVPRGRFVLFGPHPELTSRTGAHALLAKAILWAAGAKVPMEPQPSPVVQVVPKGTIPKALVAAVVATALTFVLPEDVKEELFTASDPITQGIFETISNVIQDKLGLEVPPEDLLTVTVATVVVYALEALIEWLFGTAPAPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ53</accession>
    <name>Q8TZ53_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fumarate hydratase class I, C-terminal domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fumA</name>
        <name evidence="4" type="ordered locus">MK0086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0086"/>
    <dbReference type="EnsemblBacteria" id="AAM01303">
        <property type="protein sequence ID" value="AAM01303"/>
        <property type="gene ID" value="MK0086"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0086"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.86"/>
    <dbReference type="HOGENOM" id="CLU_098588_0_0_2"/>
    <dbReference type="OMA" id="IYHCGPL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase_C" evidence="3">
        <location>
            <begin position="7"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="19245" checksum="D82F44978878FDD2" modified="2002-06-01" version="1">MTEHHLEVPLEKEERLTELEVGDVVYLSGTIITARDKAHQRIFEEGEEPPVDLEGAAVFHAGPVVRRVDDGYELVVIGPTTSTRMAKYLEDIVDAGVKLIVGKGGMGPDAPEVMKDRAVYLTAPGGCAALLAERVKSIREVHWLDLGVPEAIWVLEVEEFGPLIVTVDAHGNELAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ82</accession>
    <name>Y057_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein MK0057</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0057</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ82"/>
    <dbReference type="SMR" id="Q8TZ82"/>
    <dbReference type="STRING" id="190192.MK0057"/>
    <dbReference type="EnsemblBacteria" id="AAM01274">
        <property type="protein sequence ID" value="AAM01274"/>
        <property type="gene ID" value="MK0057"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0057"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.58"/>
    <dbReference type="HOGENOM" id="CLU_095956_1_0_2"/>
    <dbReference type="OMA" id="LRLAHMI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="DUF99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="PTHR39518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="DUF99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149234" description="UPF0215 protein MK0057">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <sequence length="197" mass="21868" checksum="7667478BE10B30BD" modified="2002-06-01" version="1">MKLREVKPEIRVLGIDDGYYGPEDDRALVVGVVMRGGQWIDGVMSTEVTVDGLDVTDRIAEMVNRSKHRPQLRVILTDGITFAGFNVLDIKKLHEETGLPVISVIKRRPDVASVVSALSNLDRTEERRKIVLRAGPVHSVKTRRDEPPVYFQCAGVEPDVARVVLKRTATRHRLPEPIRVAHFIATGVTKGESSSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ96</accession>
    <name>Q8TZ96_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA damage repair.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a predicted C4 metal binding domain at the N-terminus, which could be a zinc finger DNA binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nre family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0040"/>
    <dbReference type="EnsemblBacteria" id="AAM01257">
        <property type="protein sequence ID" value="AAM01257"/>
        <property type="gene ID" value="MK0040"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0040"/>
    <dbReference type="HOGENOM" id="CLU_039703_0_0_2"/>
    <dbReference type="OMA" id="PVGVWQI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02096">
        <property type="entry name" value="Nre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033167">
        <property type="entry name" value="Nre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006979">
        <property type="entry name" value="Nre_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006978">
        <property type="entry name" value="Nre_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38136">
        <property type="entry name" value="PTHR38136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04895">
        <property type="entry name" value="Nre_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04894">
        <property type="entry name" value="Nre_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nre_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Nre_C" evidence="3">
        <location>
            <begin position="282"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="382" mass="43247" checksum="E763E6740EA20EF3" modified="2002-06-01" version="1">MGGLCPRCRGRGWCGRDRCPFLDALNEVRARLSSRNVDGYTVTAHVSWRGYPRVIAAPGVGEHRTPDEGGVLADLPYEEALKTRALTFRRYRGKRDVRNPPDLSDPEIESSISVRPVESTLQVRRRLSGASRTTPFIGPLVEGELELDGTPKVPRDLERYHEDDVKAEEAVVGLYRRGYDESYIARALSLGLLGRDRRMVPTRWSVAAVDSMVSEHLAREVRDLPVSSEYRVLSGEVFGNEMWVILRPEPLSYELVEAYEPGAPWSDRTRIAVLRDSEVKDCKEPRETGGAYFAARLAALEWCSELGKQHGITVIRIVRKEYSAPLGAWVIREAVRRAEEVFSTDDPREVWEFLREKLDDPVGEAALKVIKRGRQRTLDTFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB3</accession>
    <name>KCY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">MK0022</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZB3"/>
    <dbReference type="SMR" id="Q8TZB3"/>
    <dbReference type="STRING" id="190192.MK0022"/>
    <dbReference type="EnsemblBacteria" id="AAM01239">
        <property type="protein sequence ID" value="AAM01239"/>
        <property type="gene ID" value="MK0022"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0022"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.22"/>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="OMA" id="ADFRFWL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132014" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <sequence length="193" mass="21886" checksum="A2357288F0609C4E" modified="2002-06-01" version="1">MFSISVVITIGGLPGSGTTTMARRLAEHYGLKHVYAGKIFREMAEERGMDLEEFSKVAEDNPDIDLEIDRRQREAAEEGDVILEGRLAAFVAAGELDHVKGPDLATLKIWLKAPLEVRAERVAKREGIDVEEARRRIQEREKSELKRYKEIYGVDPTDLSLYDLVLDTSRWSEDETFSILKAAIDPLLEREDP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>O32864</accession>
    <name>MTRD_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit D</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrD</name>
        <name type="ordered locus">MK0657</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32864"/>
    <dbReference type="STRING" id="190192.MK0657"/>
    <dbReference type="EnsemblBacteria" id="AAM01872">
        <property type="protein sequence ID" value="AAM01872"/>
        <property type="gene ID" value="MK0657"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0657"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.696"/>
    <dbReference type="HOGENOM" id="CLU_1109510_0_0_2"/>
    <dbReference type="OMA" id="HDPKFKR"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01097">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005779">
        <property type="entry name" value="MeTrfase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04207">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016552">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01112">
        <property type="entry name" value="mtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147531" description="Tetrahydromethanopterin S-methyltransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="225" mass="22872" checksum="6FA9546897670D36" modified="1998-01-01" version="1">MDKLIAVLVLITLGSIMVNVGVHYVPVGGAPAAMATATGVGTGTTQLAAGSGLTGLITAAAMSQKPFLVILWNGALGAATMMAITMLVGNFIYVYGVGCPPCSAKVDKDPITGWDQEAYVTPGTEGHGIPTVSFVSGILGGLLGGSGGAMVYYALYKVLGMSAALAGILAMGFFYANAVLASYNIGGTIEGYHDPKFTRLPKAVVCSLVFGIVASVIAYYLSTLM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>O32868</accession>
    <name>MTRG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydromethanopterin S-methyltransferase subunit G</fullName>
            <ecNumber evidence="1">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtrG</name>
        <name type="ordered locus">MK0661</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32868"/>
    <dbReference type="SMR" id="O32868"/>
    <dbReference type="STRING" id="190192.MK0661"/>
    <dbReference type="EnsemblBacteria" id="AAM01876">
        <property type="protein sequence ID" value="AAM01876"/>
        <property type="gene ID" value="MK0661"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0661"/>
    <dbReference type="HOGENOM" id="CLU_191926_0_0_2"/>
    <dbReference type="OMA" id="PQVIMDP"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005866">
        <property type="entry name" value="THM_MeTrfase_su_G"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04210">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01149">
        <property type="entry name" value="mtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147556" description="Tetrahydromethanopterin S-methyltransferase subunit G">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01500"/>
        </source>
    </evidence>
    <sequence length="74" mass="8674" checksum="3ADEDC322E3F664F" modified="1998-01-01" version="1">MAEEESVPKMVAPEDDIREIHSRLDEIERRLDFVWGEVYQRFGKRIGRDIGILYGLVIGLYLCMLYILLGVAFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q49605</accession>
    <name>MCRA_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I subunit alpha</fullName>
            <shortName>MCR I alpha</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mcrA</name>
        <name type="ordered locus">MK0655</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Int. J. Syst. Bacteriol." volume="46" first="1170" last="1173">
            <title>Phylogeny of Methanopyrus kandleri based on methyl coenzyme M reductase operons.</title>
            <authorList>
                <person name="Nolling J."/>
                <person name="Elfner A."/>
                <person name="Palmer J.R."/>
                <person name="Steigerwald V.J."/>
                <person name="Pihl T.D."/>
                <person name="Lake J.A."/>
                <person name="Reeve J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8863453"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="303" first="329" last="344">
            <title>Comparison of three methyl-coenzyme M reductases from phylogenetically distant organisms: unusual amino acid modification, conservation and adaptation.</title>
            <authorList>
                <person name="Grabarse W."/>
                <person name="Mahlert F."/>
                <person name="Shima S."/>
                <person name="Thauer R.K."/>
                <person name="Ermler U."/>
            </authorList>
            <dbReference type="PubMed" id="11023796"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.4136"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH COENZYME F430; COENZYME B; COENZYME M AND MCR SUBUNITS BETA AND GAMMA</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1 2">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid (PubMed:11023796). Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state (By similarity).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="U57340">
        <property type="protein sequence ID" value="AAB02003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="1E6V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/D=1-553"/>
    </dbReference>
    <dbReference type="PDBsum" id="1E6V"/>
    <dbReference type="AlphaFoldDB" id="Q49605"/>
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    <dbReference type="STRING" id="190192.MK0655"/>
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    <dbReference type="EnsemblBacteria" id="AAM01870">
        <property type="protein sequence ID" value="AAM01870"/>
        <property type="gene ID" value="MK0655"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0655"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.694"/>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
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        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q49605"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
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    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147454" description="Methyl-coenzyme M reductase I subunit alpha">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2 5">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </ligand>
        <ligandPart>
            <name>Ni</name>
            <dbReference type="ChEBI" id="CHEBI:28112"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 5">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAB02003." evidence="3" ref="1">
        <original>H</original>
        <variation>T</variation>
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAB02003." evidence="3" ref="1">
        <original>T</original>
        <variation>P</variation>
        <location>
            <position position="342"/>
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    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAB02003." evidence="3" ref="1">
        <original>H</original>
        <variation>Q</variation>
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    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11558"/>
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    <sequence length="553" mass="61384" checksum="F26582E0DCF2A434" modified="2002-04-16" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-07-05" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY1</accession>
    <name>RNP4_METKA</name>
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        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 4</fullName>
            <shortName evidence="1">RNase P component 4</shortName>
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        <alternativeName>
            <fullName evidence="1">Rpp21</fullName>
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        <name evidence="1" type="primary">rnp4</name>
        <name type="ordered locus">MK1622</name>
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    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
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    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TGY1"/>
    <dbReference type="SMR" id="Q8TGY1"/>
    <dbReference type="STRING" id="190192.MK1622"/>
    <dbReference type="PRIDE" id="Q8TGY1"/>
    <dbReference type="EnsemblBacteria" id="AAM02835">
        <property type="protein sequence ID" value="AAM02835"/>
        <property type="gene ID" value="MK1622"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1622"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1785"/>
    <dbReference type="HOGENOM" id="CLU_079140_3_1_2"/>
    <dbReference type="OMA" id="HVVITCL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000153853" description="Ribonuclease P protein component 4">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <sequence length="128" mass="15309" checksum="00DF43962AA9EF41" modified="2002-06-01" version="1">MCKVESPRSGVLLLRRIALERAERLLRLARTVYYEDPDRARRYVELARRIAMKARVKLPKHLKRSFCKRCNTPLIPGVTARVRLRQNRMPHVSVTCLECGYIYRYPYLREVKERRRRHMEGVKDRDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY7</accession>
    <name>Q8TGY7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted extracellular polysaccharide hydrolase of the Endo alpha-1,4 polygalactosaminidase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02338">
        <property type="protein sequence ID" value="AAM02338"/>
        <property type="gene ID" value="MK1125"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1125"/>
    <dbReference type="HOGENOM" id="CLU_1324028_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_114" evidence="1">
        <location>
            <begin position="74"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="37527" checksum="BD66851D99CB6A72" modified="2002-06-01" version="1">MSVGPAHSFEPVHTEPPGECTSLKPLLTIFLAFTVIDHGVPLATYWQDVNLRVILEKRPRTLVVDPYCGPGDRPWTEDELRTLREHGVKPVAYLSLATVGEHQRDLYRLTRSKRLLGRPDPEWPGDHAVRFWERAWLDALRAKLKELRDLGYEGVFIDVVDPWSRDWYVEWFHRETGGDVDELKGRSYRALEELLRTARDLGLRVYVNAGDAVFDRRLAELKDRYGFGVVVENVVADEEGRPTPGNVFEAKLRALERLGDWVYVFEYGIDPERERNRLTELFSRTDVEEVYITSPDHDRLGKAAVPPTALLKRPGSTMGRLLKLLVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ5</accession>
    <name>ENDAL_METKA</name>
    <protein>
        <recommendedName>
            <fullName>EndA-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0397</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Although strongly related to the EndA enzyme, it does not contain the conserved Tyr-His-Lys active sites, preventing tRNA-splicing activity.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="5X89">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.53 A"/>
        <property type="chains" value="A=2-166"/>
    </dbReference>
    <dbReference type="PDBsum" id="5X89"/>
    <dbReference type="AlphaFoldDB" id="Q8TGZ5"/>
    <dbReference type="SMR" id="Q8TGZ5"/>
    <dbReference type="STRING" id="190192.MK0397"/>
    <dbReference type="EnsemblBacteria" id="AAM01612">
        <property type="protein sequence ID" value="AAM01612"/>
        <property type="gene ID" value="MK0397"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0397"/>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="OMA" id="ENINAIH"/>
    <dbReference type="BRENDA" id="4.6.1.16">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="PTHR21227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="SSF53032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="SSF55267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000109483" description="EndA-like protein">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="40"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="93"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="102"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="112"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="144"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="156"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="5X89"/>
        </source>
    </evidence>
    <sequence length="166" mass="19413" checksum="80A7D613FFEBE3AF" modified="2002-06-01" version="1">MAAKGELVGSKVLVRNDRDANRLYSSMYGKPSRRGLQLWPEEALFLCEIGRLEVRSGNVRISPEELMDRFVEEDPRFPVRYAVYADLRRRGWKPKPGRKFGTEFRAFRGEDERIAVKVLQEELDEFTAQDILEWLKLVEGTEFELVVAIVDNDYDLNYYVFSELVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR8</accession>
    <name>Q8TUR8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1686"/>
    <dbReference type="EnsemblBacteria" id="AAM02899">
        <property type="protein sequence ID" value="AAM02899"/>
        <property type="gene ID" value="MK1686"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1686"/>
    <dbReference type="HOGENOM" id="CLU_074390_5_0_2"/>
    <dbReference type="OMA" id="DIVIYPS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05126">
        <property type="entry name" value="Mth938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034096">
        <property type="entry name" value="AAMDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="SSF64076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="123" mass="13801" checksum="74F7B08BCEE0D302" modified="2002-06-01" version="1">MFEGTGFGWVEYGGERYNHDIYVTTEGKVHRREKGLSRSKFGTSHNLAADELRRLLELCDEEPEVIVVGTGQSGVLSVTEEAREFCEERGIELVEAPTPEAIERYNELKDKGKKVAAVIHTTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUT5</accession>
    <name>Q8TUT5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02881">
        <property type="protein sequence ID" value="AAM02881"/>
        <property type="gene ID" value="MK1668"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1668"/>
    <dbReference type="HOGENOM" id="CLU_159088_0_0_2"/>
    <dbReference type="OMA" id="MGSEVCD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019209">
        <property type="entry name" value="DUF2098"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017099">
        <property type="entry name" value="UCP037053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09871">
        <property type="entry name" value="DUF2098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037053">
        <property type="entry name" value="UCP037053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="89" mass="10441" checksum="C57C08CB909AFA7E" modified="2002-06-01" version="1">MTELQVGDYARYVRTGTVGKVVDVKEREDGRWIQLDSTGLYYHEDYVEKVEKKESKEKEETDIEEVVERIRELKEAFEHVDERVCEGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU6</accession>
    <name>Q8TUU6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1657</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1657"/>
    <dbReference type="EnsemblBacteria" id="AAM02870">
        <property type="protein sequence ID" value="AAM02870"/>
        <property type="gene ID" value="MK1657"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1657"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1821"/>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="OMA" id="AEHASTF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="PTHR40696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="140" mass="15733" checksum="F5555FAD6508BF14" modified="2002-06-01" version="1">MEITLKARGHENVTGRHRSTFEVTKDPEIGPTADCIIGVRADTACADLPEDFKLMLRRGARVEVILRAGGTEDRVVGVGHPRMTLQDGRSMVFRTSDYIDDRTVLIRCNKAARDLSRDLVRALKDPETVLMVEMRVLDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU7</accession>
    <name>Q8TUU7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Archaea-specific Zn-finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1656"/>
    <dbReference type="EnsemblBacteria" id="AAM02869">
        <property type="protein sequence ID" value="AAM02869"/>
        <property type="gene ID" value="MK1656"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1656"/>
    <dbReference type="HOGENOM" id="CLU_110112_1_0_2"/>
    <dbReference type="OMA" id="KHERSFK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="PTHR42195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26585" checksum="EDF4D9788FED9F46" modified="2002-06-01" version="1">MITLDTETTVEDLSESDVEGRIIYAPCKGCSPDEEVPQEVVRATVHRKGPDEKGVPRFVANVLARCESCGTVNPVRLVFYAPKKVKVTISRYEESECKEVEMDPLEEIEVGDVIEVEGERVEITNIATHEEDSVKKAKVKDVVSVWGVSLDIPARIGVSINLPSGYTISEKVEVDRDEEFTVGEIYELDGMLFRVHAIKPEGRPTVKREGESVKAEEIKRIYGRPVRRGTPKKSLESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW3</accession>
    <name>Q8TUW3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02853">
        <property type="protein sequence ID" value="AAM02853"/>
        <property type="gene ID" value="MK1640"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1640"/>
    <dbReference type="HOGENOM" id="CLU_996090_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="279" mass="33061" checksum="BD7DF27700C042BE" modified="2002-06-01" version="1">MALEKLADLLDVRNENTRHSYRFALRRLDEFLRRILEDEEPEEWLEKAPDEEILEVARELRNLLVESVRNMELDHEMGRLCWYALTKALRAVGRDPLLVLTVKAYGSPASPEMTEQDAREILELVDLIEEWAKENKDASWWAPFRIIAESGISVMDLVRMRWADVCPGEPWLEETPDEPRLRVEKPEETIEFPITERGLEALRELAEWAGVDPTEEPERPILMSEDDEYHTDAQRMDSWRARLTYRWREAQRSVLGESEWRIKDLTRATRILRLPELRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW5</accession>
    <name>Q8TUW5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Orphan DOD family homing endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1638"/>
    <dbReference type="EnsemblBacteria" id="AAM02851">
        <property type="protein sequence ID" value="AAM02851"/>
        <property type="gene ID" value="MK1638"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1638"/>
    <dbReference type="HOGENOM" id="CLU_753579_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="1">
        <location>
            <begin position="69"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="367" mass="40362" checksum="7BE225622BA8B5AD" modified="2002-06-01" version="1">MNPELILARLFGVLLGRGTFRDGDRPRVRLSVPIGGASELLADLSRLGVNPSVRCGKRWTSVESRDGRLIGALIMAGASADPVDGIPNWVFEDPEAARELLSGLYSVRGRVLGFDGSRPRPVRLELRARADRELDLLSLALDVCRLLGSLNVDAEVVGLSHVSSGHYRYARVEVLIRGRRDLRRFLSEVEFRYNPEQSERARRMLRELARRETQLSTKWPARWGDVGVSDSGDEGLVLRTELGFEIAGPADMWVSSRNKRRLGDLKPGDRVVLYPVGVPPAADRRGTLLADVDAPEGARRFLRDLDLLPLRWEDPALPTLTRVLGYAIGDGHLEPDVVVLRGAGGTAVARGRSARARPLAGAVRVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX3</accession>
    <name>Q8TUX3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02842">
        <property type="protein sequence ID" value="AAM02842"/>
        <property type="gene ID" value="MK1629"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1629"/>
    <dbReference type="HOGENOM" id="CLU_2340196_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="97" mass="10710" checksum="A5D940FA85AD7B7D" modified="2002-06-01" version="1">MRWKDNAKKYKHQLTGRPYDGALVVWGLDPDPRSDFYVAGGKLLVIRCEESGGSITVEFVDAADPGNLPDWVSGSVDLGWLFDRSSGEGTPTLFVYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX4</accession>
    <name>Q8TUX4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02841">
        <property type="protein sequence ID" value="AAM02841"/>
        <property type="gene ID" value="MK1628"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1628"/>
    <dbReference type="HOGENOM" id="CLU_784397_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="353" mass="40217" checksum="0A2E9B65FE8405C8" modified="2002-06-01" version="1">MAERIVREVLREARRRGVSVSDLFSSEGWVVNEITDRISRVLGSVGAPSEDVRESILEETSSKLKSPTTYLSSIAETVSQVLPWGEEDADALPDHDFSEKVLDHLLEGAEEFVCLASPWVSSPKDLVEEGVRSLRGLSDRDLELYLLVAAGENEREVLLEWASLGFEVREAEGRREGDLGIHCKVYANEKLALGASWNLTVSSLRRLRAMREVHTINPKADDCEVCNANYEQLVSEFNSQWSEAKQRFFRDEGLKGPAIFEVRWDGDRPTEVVIYRENGERWFTVELEDNHEGFVFNEREGLPVRRGVPRVHRFDLRGSRRRGQDRLSLRRTQAREDPSGASGCRRAGREMER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY1</accession>
    <name>Q8TUY1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">30S ribosomal protein S19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps19A</name>
        <name evidence="4" type="synonym">rps19e</name>
        <name evidence="5" type="ordered locus">MK1620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1620"/>
    <dbReference type="EnsemblBacteria" id="AAM02833">
        <property type="protein sequence ID" value="AAM02833"/>
        <property type="gene ID" value="MK1620"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1620"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1783"/>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="OMA" id="YIDGPVG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_S19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_S19e_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_S19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="PTHR11710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="149" mass="17171" checksum="38DF70FAA6E2887A" modified="2002-06-01" version="1">MYDAYVVPGSELVERLAEKLKDFEEIKPPEWAKYVKTGRHKERPPEDPDWWYMRAASILRRVYMDGPVGVSRLRTYYGGRQDRGARPERFRKGSGAIIRKILQQLEEAGLVEKTEEGRVVTPEGRSLVDSTAHEIAKEKGYTDKFTSPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY6</accession>
    <name>RL18A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18a</name>
        <name evidence="1" type="synonym">rpl20e</name>
        <name evidence="1" type="synonym">rplX</name>
        <name type="ordered locus">MK1615</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY6"/>
    <dbReference type="SMR" id="Q8TUY6"/>
    <dbReference type="STRING" id="190192.MK1615"/>
    <dbReference type="EnsemblBacteria" id="AAM02828">
        <property type="protein sequence ID" value="AAM02828"/>
        <property type="gene ID" value="MK1615"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1615"/>
    <dbReference type="HOGENOM" id="CLU_177460_0_0_2"/>
    <dbReference type="OMA" id="MVFTKEY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_L18Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="50S_L18Ae/Ribosomal_L18a/L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_L18a//L18Ae/LX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000153699" description="50S ribosomal protein L18Ae">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <sequence length="80" mass="9232" checksum="069344A84152609F" modified="2002-06-01" version="1">MSEVKVFEVRGTFRMGDEPRQPFTRQVPATSEEEALEKVYSDLGSEHGVSRMEIQIEEIREIDPSKVEDPILRRLLGVEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-19" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ9</accession>
    <name>MFNB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">(5-formylfuran-3-yl)methyl phosphate synthase</fullName>
            <ecNumber evidence="1">4.2.3.153</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase</fullName>
            <shortName evidence="1">4-HFC-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mfnB</name>
        <name type="ordered locus">MK1602</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="EC" id="4.2.3.153"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MfnB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.153" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUZ9"/>
    <dbReference type="SMR" id="Q8TUZ9"/>
    <dbReference type="STRING" id="190192.MK1602"/>
    <dbReference type="EnsemblBacteria" id="AAM02815">
        <property type="protein sequence ID" value="AAM02815"/>
        <property type="gene ID" value="MK1602"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1602"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1764"/>
    <dbReference type="HOGENOM" id="CLU_068659_0_0_2"/>
    <dbReference type="OMA" id="NFPWVIR"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00681">
        <property type="entry name" value="MfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007565">
        <property type="entry name" value="4HFCP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035081">
        <property type="entry name" value="4HFCP_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04476">
        <property type="entry name" value="4HFCP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015957">
        <property type="entry name" value="UCP015957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000134866" description="(5-formylfuran-3-yl)methyl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00681"/>
        </source>
    </evidence>
    <sequence length="237" mass="25404" checksum="BFC1DBD64FEEA762" modified="2002-06-01" version="1">MRPRLLVSPVNRDEALEAVEGGAHIIDVKNPEEGSLGANFPWVIREIMEVVPEDREVSATVGDVPYKPGTVAQAVLGVAAVGVDYAKVGLYGTKTEEEALEVMRACSRAVREFGYDTRVVAAGYADAHRVGSLDPMSVPEVAAEAECDVAMVDTAVKDGKRLFDFLSEEEVGEFVDSAHEHGLEVALAGSLRHEDMPIVRDLGADIVGVRGAACERGDRNRGAIRSHLVRKLAEALA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV03</accession>
    <name>RL7A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl7ae</name>
        <name type="ordered locus">MK1598</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV03"/>
    <dbReference type="SMR" id="Q8TV03"/>
    <dbReference type="STRING" id="190192.MK1598"/>
    <dbReference type="EnsemblBacteria" id="AAM02811">
        <property type="protein sequence ID" value="AAM02811"/>
        <property type="gene ID" value="MK1598"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1598"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1759"/>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="OMA" id="KPFYVRF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_L7Ae_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_L7Ae/L8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_L7Ae_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_L7Ae_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000948">
        <property type="entry name" value="Ribosomal_L7Ae_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136794" description="50S ribosomal protein L7Ae">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <sequence length="123" mass="13485" checksum="D3A428E4BEE9F237" modified="2002-06-01" version="1">MSKPMYVKFEVPEELAEKAYEALEIARDTGRIRKGTNETTKAVEREEAVLVLIAEDVDPEEVVAHLPELCDEKGIPYVYVPSKDELGAAAGIDVAAASACIIDPGDAKDLVDEIIEKVEELRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV17</accession>
    <name>Q8TV17_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02797">
        <property type="protein sequence ID" value="AAM02797"/>
        <property type="gene ID" value="MK1584"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1584"/>
    <dbReference type="HOGENOM" id="CLU_1357922_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="201" mass="22655" checksum="B27952A65570B678" modified="2002-06-01" version="1">MRPVVWTVRIAAAAIILVAMTASGFLFAVSLTGKSPVDPQLVMRMSSDLARGDYDAFNRDFAMAYLQLTSRLHEGLQTLLEYLGIKAKFIKEEVKKKLFRYRVRVVNSEDVCTKLTELAHVDDIAKGKLMVYADTKSEPNKGTKVLIKAIGYVEKVKIKGETIIIYSTPYYIEAEGMGIYNGDKEVIIDVNTIKLRQVRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV20</accession>
    <name>Q8TV20_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted deacylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1581"/>
    <dbReference type="EnsemblBacteria" id="AAM02794">
        <property type="protein sequence ID" value="AAM02794"/>
        <property type="gene ID" value="MK1581"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1581"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1742"/>
    <dbReference type="HOGENOM" id="CLU_021802_2_0_2"/>
    <dbReference type="OMA" id="LNSHHDT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="174"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="381" mass="40728" checksum="4637013709C50A83" modified="2002-06-01" version="1">MSGNPSGALQRVNDLELDPIGLLKDAVATPSVTGEEHEMTRLLTEVLDEHGVPYEVDEMGNVLAGDLSGLVLNAHLDTVPPGDGWEVTDPFDPTIRNGKLYGRGAADCKGGLAAATAAVVQGYYEEMPMGLLATVGEESSSEEDNGTLHVCRTRELEARAGIVCEPTDGRVHVGDRGRITLRVTVRGRSAHASTPEMGKNPIEAASRVVEALSKLRPTEYRLPEIGTVRSDLTVTRIEADGPSNVIPERCEMTVDYRTVPGESTKEVKRRVERVAKRAVPSGFEVSVGIESASRATVVNVEAPVVKAAVIAARKVGLPGKLDFKRGHCDIEYLVHEAGLDAVILGPSGGNIHGPDEWVKVEDVVRCARAYLACANLLPTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV54</accession>
    <name>Q8TV54_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ni2+-binding GTPase involved in regulation of expression and maturation of hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hypB_2</name>
        <name evidence="2" type="ordered locus">MK1546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1546"/>
    <dbReference type="EnsemblBacteria" id="AAM02759">
        <property type="protein sequence ID" value="AAM02759"/>
        <property type="gene ID" value="MK1546"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1546"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1707"/>
    <dbReference type="HOGENOM" id="CLU_056148_0_1_2"/>
    <dbReference type="OMA" id="ECHLDAH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00073">
        <property type="entry name" value="hypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="31"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="218" mass="24068" checksum="FD5428BAD9CB9AD9" modified="2002-06-01" version="1">MHKVEVDVSEDLLEVNRNLAREVRETLDEHNVRAVEVLGSIGSGKTSLIEWIVKEYGDEYSFAVIAGDVVSEYDERRFKDLGVPTVGLNTGRECHLDAHMVQHGLEHLEELTDLNEVDVLFIENVGNLVCPADFPIGAHLRVIVVSATEGEDVIGKHPMMIRKGDVLVVNKIDLADACGVSPETMVRTAKEINPDLEVYLTSIKTGEGMAELAERLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV66</accession>
    <name>Q8TV66_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02745">
        <property type="protein sequence ID" value="AAM02745"/>
        <property type="gene ID" value="MK1532"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1532"/>
    <dbReference type="HOGENOM" id="CLU_443881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="592"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="514"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="615" mass="66941" checksum="77729702806BC922" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV67</accession>
    <name>Q8TV67_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02744">
        <property type="protein sequence ID" value="AAM02744"/>
        <property type="gene ID" value="MK1531"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1531"/>
    <dbReference type="HOGENOM" id="CLU_2313801_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012755">
        <property type="entry name" value="UCP012755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="99" mass="11458" checksum="38824FEF6DD6FA16" modified="2002-06-01" version="1">MKTLRMSPEELLEYFRKEVEEGDRLELNYAFVHIPGEVVKITRYRLHLYVESEVAPGIRRYDLEEICSMMLEVIHEPKEGKPVKIVVEGADESPGIGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC5</accession>
    <name>Q8TVC5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1467</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02680">
        <property type="protein sequence ID" value="AAM02680"/>
        <property type="gene ID" value="MK1467"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1467"/>
    <dbReference type="HOGENOM" id="CLU_2420058_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008032">
        <property type="entry name" value="DUF749"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035933">
        <property type="entry name" value="MTH1880"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016458">
        <property type="entry name" value="UCP005648_Ca-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05370">
        <property type="entry name" value="DUF749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005648">
        <property type="entry name" value="UCP005648_Ca-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75412">
        <property type="entry name" value="SSF75412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="91" mass="10533" checksum="075E0DF8EAA9341B" modified="2002-06-01" version="1">MFVAKYLGSARYSELDEELKDFARLKAHLAGVDLNRDPELMIFNVEGTSSYYIVFLEEVESEEDVERLLREDMGAELSRDSKASVRRVLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG9</accession>
    <name>Q8TVG9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02633">
        <property type="protein sequence ID" value="AAM02633"/>
        <property type="gene ID" value="MK1420"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1420"/>
    <dbReference type="HOGENOM" id="CLU_1127096_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="27574" checksum="C59CE68CC59667B7" modified="2002-06-01" version="1">MIGALLASHSYPTAVTWDILAEIAEILPSSITISCTDPDIWEFCIELRDHVEFLDVEEVDSLDPTTVVALAEEEAHMGPKVLRTRVRGVVIVTDDPERAAEDWRLPAVSAREIYVPVVRVGGNATVHVYRHGEVLWRLGLREGKTLLPPDYKSLRELLWNEFPYVRTIGSLAQRTNWHEVLVRLALIEEWIKGNKLVSEFLDWIPKSTKVIPRSTDSPTDSSSGENLLEGRLILIPAFSSTLSALA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ1</accession>
    <name>RIFK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Riboflavin kinase</fullName>
            <shortName evidence="1">RFK</shortName>
            <ecNumber evidence="1">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribK</name>
        <name type="ordered locus">MK1398</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CTP + riboflavin = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVJ1"/>
    <dbReference type="SMR" id="Q8TVJ1"/>
    <dbReference type="STRING" id="190192.MK1398"/>
    <dbReference type="EnsemblBacteria" id="AAM02611">
        <property type="protein sequence ID" value="AAM02611"/>
        <property type="gene ID" value="MK1398"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1398"/>
    <dbReference type="HOGENOM" id="CLU_140165_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="PTHR40706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000322075" description="Riboflavin kinase">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <sequence length="147" mass="16880" checksum="1FBFA65B4D2AB012" modified="2002-06-01" version="1">MFTGPIVAVGEYVEGLGEGRRYVSIPYYRREIERVIGARPFPGTFNVRVEREERESLRELASSYGYRIEPHGEYGGAWLYPCLVNGRPAWLVFPDLTEHRDQVELISETELRRELNVIHGDMVKIEVWGPSTWKLARRLTCGPSGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK3</accession>
    <name>Q8TVK3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Di-and tricarboxylate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">citT</name>
        <name evidence="8" type="ordered locus">MK1386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02599">
        <property type="protein sequence ID" value="AAM02599"/>
        <property type="gene ID" value="MK1386"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1386"/>
    <dbReference type="HOGENOM" id="CLU_063025_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="CitMHS" evidence="7">
        <location>
            <begin position="15"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="247" mass="26385" checksum="02FAF075E0E3A2A0" modified="2002-06-01" version="1">MLPSSPSCRCWLRAPRRPHRVVLPALVSANSVGALTPLGNPQNAIIYSHYRVDPVDFFVTQLPVCAALLAPGLLYAWVRGERVESGSGSAPNVLDVAVVIAAAGCLLAHVPVYFWFPAVFGYYAVLRPHAVREVDWVVIGLLTVGVLVPSVIGSLGWNPRVDDPFVWSVLLSQVVSNVPTTVLVVPMTDDWRDLLHGVTVGGYGTPVASVANLIALRAAGSRGVLRDYAVLQGSCLMLGVLSHYALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ7</accession>
    <name>Q8TVQ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK-11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02544">
        <property type="protein sequence ID" value="AAM02544"/>
        <property type="gene ID" value="MK1331"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1331"/>
    <dbReference type="HOGENOM" id="CLU_619135_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="442" mass="50140" checksum="BD791879361C8AF3" modified="2002-06-01" version="1">MRRVLSPAIVPIVVLVLPLPVHGDSWTDPEALLDVGVQSHLVIEDVHEKEVTTEHGDTVKVLDYVDATYMAVIKAKEPGKPVTVHVLLPQGTEVLEARFYPKVSDRSPSEVLSDPSRFESVKVKPKGQTEVTWEFLDETWKWTQLDFEVTPEHDPGKPFSYSLLVLKLRIPALHRVPNLRMYYPKHYDDALRSEYSYGVIEWPIPAQPGYDNSFYVRSGSETLDVGVELFAWPEGVVTKSSAAPGWDTCQARLTDHLMLVFPEARVAVLADVSPAEDYEKWKHQREEVIEKEWPQVTQRFGFPYVPVVAVIGVERVEVSFDLLVPERVDAGSTVTVFVRPYPEPWSYAPEEGVLRFELRDEHGSVMELGEVKLPEGSGFEGWVEFRFRAPEKPGYYTLFVEYEGPYGNGKTSATFRVEGRGKRCKLPIPVVPVIPPRRRARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ9</accession>
    <name>Q8TVQ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-32 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02542">
        <property type="protein sequence ID" value="AAM02542"/>
        <property type="gene ID" value="MK1329"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1329"/>
    <dbReference type="HOGENOM" id="CLU_808010_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="343" mass="37645" checksum="98A7CAB73A1580FE" modified="2002-06-01" version="1">MAVDRDLCPDADLRKVVEALGFFFDPVRAVGLALNLRGHMCFVDGPGAGKTTFLDAFSVAVVSGEGVTREAMVWDERTDRPGVVLHYPAGFVGWDECDSIGSPEVRKLVLEVLDFRLEVVAHGELRTVRHNRRFLFAGRAVPAFPEVDLIRFLGRMQYIGGVGGDIRDRVLSGAVDPRAVEALYERISWDVQAVHLAGCAAEGPFVSGEFAEEIFRGYLRELREAFAAALLEAAKRFPIGAGAGLARKIAMAYVQDKINRFADTVIAFGGPEAVADVVREYYALCDLEAPEDDVECLVHAWVNALMNNYSQWCAEGKIPGPKIAYETIASEDDLDLRFEDVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS2</accession>
    <name>Q8TVS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms endoribonuclease Csm3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1316</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2013" name="RNA Biol." volume="10" first="1670" last="1678">
            <title>Structure and RNA-binding properties of the type III-A CRISPR-associated protein Csm3.</title>
            <authorList>
                <person name="Hrle A."/>
                <person name="Su A.A."/>
                <person name="Ebert J."/>
                <person name="Benda C."/>
                <person name="Randau L."/>
                <person name="Conti E."/>
            </authorList>
            <dbReference type="PubMed" id="24157656"/>
            <dbReference type="DOI" id="10.4161/rna.26500"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.37 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="4N0L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.37 A"/>
        <property type="chains" value="A/B=1-351"/>
    </dbReference>
    <dbReference type="PDBsum" id="4N0L"/>
    <dbReference type="SMR" id="Q8TVS2"/>
    <dbReference type="EnsemblBacteria" id="AAM02529">
        <property type="protein sequence ID" value="AAM02529"/>
        <property type="gene ID" value="MK1316"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1316"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1451"/>
    <dbReference type="HOGENOM" id="CLU_067743_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013412">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02582">
        <property type="entry name" value="cas7_TM1809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4N0L"/>
        </source>
    </evidence>
    <sequence length="351" mass="39570" checksum="A2064BAC439C2DB5" modified="2002-06-01" version="1">MVGIGGTITLVGEIRLRTGTRIGTSEEEIEIGGLDNPVIRDPVSGYPYVPGSSLKGRARALFELAWMKSREIEPDVFFGAHHNERHECGFVRREVYEEAKEYLREDPPWLENGTCPVCRIFGSAGDGIGFSDPGRLEDERRGLGYDPYGRYRDPNDAQELSGVVDVKKEARVAFRDAHPTTYTVNDVFERAGEPTEVKHENAINRVSGEANPRSMERVPKGSRFGLEVVYRVEDGEELESDLKYLMSSLKLVEDQGIGHSTSRGYGRVEFRIAALCARSTGWYLDPGAGEGFPEEEDKDEAADEVTYLSDLEAERYEIVIRARDLEDRAYLRPEEWVERLDEVVGELPWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT0</accession>
    <name>Q8TVT0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted P-loop ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1308</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02521">
        <property type="protein sequence ID" value="AAM02521"/>
        <property type="gene ID" value="MK1308"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1308"/>
    <dbReference type="HOGENOM" id="CLU_428040_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="639" mass="71563" checksum="8F070CFDFDC9F805" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX4</accession>
    <name>Q8TVX4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-37 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02477">
        <property type="protein sequence ID" value="AAM02477"/>
        <property type="gene ID" value="MK1264"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1264"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1375"/>
    <dbReference type="HOGENOM" id="CLU_502159_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="492"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="542" mass="60672" checksum="9E3CC32735F10A8D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ3</accession>
    <name>Q8TVZ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02458">
        <property type="protein sequence ID" value="AAM02458"/>
        <property type="gene ID" value="MK1245"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1245"/>
    <dbReference type="HOGENOM" id="CLU_040681_9_3_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="70"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="122" mass="12918" checksum="7073EE284D1FAAC7" modified="2002-06-01" version="1">MPTVRDAMTEDVVVVGPDEPLERVLRTFASESIHGVPVVEGGRLIGIVTSVDVVRALASGEWRELTAGDVTRKAVTVDPDEDLETALDLMAAVGEDRAVVVEDGEIVGVVTVLDAIRVLLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ5</accession>
    <name>Q8TVZ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02456">
        <property type="protein sequence ID" value="AAM02456"/>
        <property type="gene ID" value="MK1243"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1243"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_6_2"/>
    <dbReference type="OMA" id="FGCKRCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="60" mass="6270" checksum="5C058199BB9F823F" modified="2002-06-01" version="1">MFVHDASECEGCGECMRACPTGALLVSVGYVEVDASKCRACGSCARVCPTGALRMEPKRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW18</accession>
    <name>RS4E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4e</name>
        <name type="ordered locus">MK1220</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW18"/>
    <dbReference type="SMR" id="Q8TW18"/>
    <dbReference type="STRING" id="190192.MK1220"/>
    <dbReference type="PRIDE" id="Q8TW18"/>
    <dbReference type="EnsemblBacteria" id="AAM02433">
        <property type="protein sequence ID" value="AAM02433"/>
        <property type="gene ID" value="MK1220"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1220"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1323"/>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="OMA" id="FPVGLMD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="KOW_RPS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_S4e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_S4e_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_S4e_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_S4e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_S4e_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="PTHR11581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130850" description="30S ribosomal protein S4e">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="46"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <sequence length="260" mass="29474" checksum="DB126C89C7ADD0BC" modified="2002-06-01" version="1">MGRARSGPKRHVKRLAAPYAWPIPRKEGGKFAPRPYPGPHTMDTSVPLLILVRDMLGYADYAREARKIITRGEIYVDGVVRKEPKFPVGIMDVVEIPRTGDRYRVVMNEHHRLDVVPISEEEARVKVCRIKNKTYVRGGNLQITMHDGKNWLVEIEDPTDPKEDVYSVGDSLVLELPEPDSGESWKVVDHIPFEEGVWVYAMTGRHSGEVGRVVEIQTFEGPQEDLITVENPEGDQFQTTKGRLIAIGKDEPLVTVRKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW37</accession>
    <name>Q8TW37_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized, MobA-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1200"/>
    <dbReference type="EnsemblBacteria" id="AAM02413">
        <property type="protein sequence ID" value="AAM02413"/>
        <property type="gene ID" value="MK1200"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1200"/>
    <dbReference type="HOGENOM" id="CLU_1264578_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="2">
        <location>
            <begin position="8"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="231" mass="25026" checksum="B05553E48CE95390" modified="2002-06-01" version="1">MRTLRVAACVLAAGRSTRFREALEERGEEPVSKLVYPVAGKPMVAWVVERASRVVDELLVGLGYDAGLVWDVVRQHATVPARPVLNDPVDVPMARTAANLLRRSDADVSLILAGDQPTVTAETMRRLAETAAEHGASVLDRGAPNEEVSGDDLLGHGPPLALRKDVVPEFLETIEELAADVRGPNALNLNPVLRECGLAFRVVPPRDDAELLNVNTPDELPEVERALLRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW60</accession>
    <name>Q8TW60_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02389">
        <property type="protein sequence ID" value="AAM02389"/>
        <property type="gene ID" value="MK1176"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1176"/>
    <dbReference type="HOGENOM" id="CLU_2032881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="121" mass="13168" checksum="19F314CA20108688" modified="2002-06-01" version="1">MSVPKYLPLLIAVVATASPASGYLGVEAFVTGISADHVDVVYMVYADSDDVTKSHRVTLYLPPEATIKGVKSYQLPRREGDVDVVYGQGDWKPETGTWSETARSDPRSSFPKRPPMSSTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW81</accession>
    <name>Q8TW81_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02368">
        <property type="protein sequence ID" value="AAM02368"/>
        <property type="gene ID" value="MK1155"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1155"/>
    <dbReference type="HOGENOM" id="CLU_1006895_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="276" mass="29245" checksum="2123477E03A99DCF" modified="2002-06-01" version="1">MRTSTPDDRKLRDCTAEVGVLSVLGRIVVSHRLLESLGSDDRDYLRYLRCALISDLVACVSLLGVLEIYAFSLTVSPAAIPVAIPAILLLAGMTYLLKTLLESSPLAGVVDRITYHMCILSAGTCDPVISMQLQPPGDLMDIPPYAFGLVYGLTVLAPIILLFGTLMPAGAVLEPFEYPLEDSPIPGLILVADIVRPFTLCTVILDVLSEVLSAVLLPGEVVRKAVAALGRSVMSLLGIGPSGEGARAEPAGGSGAPRASLNAHRYSLWNPLQITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW96</accession>
    <name>Q8TW96_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02353">
        <property type="protein sequence ID" value="AAM02353"/>
        <property type="gene ID" value="MK1140"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1140"/>
    <dbReference type="HOGENOM" id="CLU_2597747_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="8571" checksum="1037B2DC39E23698" modified="2002-06-01" version="1">MIPAVVGTGVGAATVLGSLPPYCGWLAPAAMLTGFLLTLKYERWVRDHRIVTVWGRVVETVRYYPWPSRSTRSAPGGGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD0</accession>
    <name>Y1105_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel insertion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1105</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LarC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWD0"/>
    <dbReference type="SMR" id="Q8TWD0"/>
    <dbReference type="STRING" id="190192.MK1105"/>
    <dbReference type="EnsemblBacteria" id="AAM02318">
        <property type="protein sequence ID" value="AAM02318"/>
        <property type="gene ID" value="MK1105"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1105"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1160"/>
    <dbReference type="HOGENOM" id="CLU_028523_2_1_2"/>
    <dbReference type="OMA" id="EILCKHE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01074">
        <property type="entry name" value="LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="PTHR36566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="DUF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00299">
        <property type="entry name" value="TIGR00299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146861" description="Putative nickel insertion protein">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01074"/>
        </source>
    </evidence>
    <sequence length="395" mass="43478" checksum="21A82922377B8D99" modified="2002-06-01" version="1">MILTLDPQVAGASGDMVLGALIAVGADPNRLEEVVHEVSSLGHEVDVHVHEIQKRGIRAVRVEVDAEGDLRDPDELREAVKTVAENVLEDRWRELPELALKYLLRAEERVHGDLCHLHELGSSDTVVDLVGTAALLEDLNPKASEVLPPNVGSGTVETEHGRLPVPAPAVVEVLSEWDVGIVREGEGELLTPTGAALLRTIDELLPDPPPPYRVKRQGFGAGTKDLPDRPNVLRALICEPGGSGEHVRIVETSVDDVDGEAVGELIEAVLQLEGVHDVEVLHGFGKKGRPRFVIRVVTEDRPGIEREVFRELFRWTGTLGARVYRCTRVTADRRIVDVDGIRVKVSRFEDVHHAKPEWEDVRRKVDRESAPLTRARLVGDLRKRYEGDGEDGAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF4</accession>
    <name>Q8TWF4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="9">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">miaB</name>
        <name evidence="13" type="ordered locus">MK1081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="9"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1081"/>
    <dbReference type="EnsemblBacteria" id="AAM02294">
        <property type="protein sequence ID" value="AAM02294"/>
        <property type="gene ID" value="MK1081"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1081"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1136"/>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:N6-threonylcarbomyladenosine methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061712">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="132"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="10">
        <location>
            <begin position="369"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAM02294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="423" mass="48360" checksum="4072CC49AD70A2CA" modified="2002-06-01" version="1">MTKVAVEVYGCAANHDDGRLVRELLRREGFEVVEDAENADVAVLLTCIVRDSVDARMVNRMRELERVPTVVAGCFPEAYPERARKLRPDAALVGPRHLDRIPEAVRAVLRGDRVEFLGEREDIDWKADAPRELPNLAAIVPIAEGCPNRCAYCAVKLARGNLRSFPPERILRRVKRELERGAVEIHLTAQDTATYGLDRGTNVVELLEDVVDLCSRYGARVRLGMFNPGHAYPISDDLADLFASRDDVLYRSIHMPVQSGDDEVLRRMNRNYTVEEALEVYRAFERRLGYFSFITDVIVGFPGETEEAFRNTLRFLERTRPHILHASRFCRRPGTPAARMEDQVPEDVKLRRSRILHRKRLEWAEEANRELIGETVEVTMVMEKWGRDEHAKKTVFRGEVPEPGERLECRIVDASHARLVAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG1</accession>
    <name>Q8TWG1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1074</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1074"/>
    <dbReference type="EnsemblBacteria" id="AAM02287">
        <property type="protein sequence ID" value="AAM02287"/>
        <property type="gene ID" value="MK1074"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1074"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1128"/>
    <dbReference type="HOGENOM" id="CLU_023791_0_0_2"/>
    <dbReference type="OMA" id="RCDIHYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="PTHR43306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="87"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="499" mass="56619" checksum="4655980C1277BF7A" modified="2002-06-01" version="1">MSDTLYETESLCPECLRVVPARLVRTAEGSVEIVKKCPEHGEFREVVWSDVEFFERAFEYEFKGPGVENPQTDSENGCPLDCGLCPCHESTTALGIIDVTNRCNMNCPVCFANAEAKGYVYEPSLEQIEEMLDLLRSERPVPAPAVQFAGGEPLVREDIVEIVAAADERGFHVQIATNGVEFARNPELAEDLHAAGLNVVYLQFDGLNPEIYEEIRGSRKVLELKKEAIKVLEREGISTVLVPTLARGVNDDQIRPMLDFAREFEVIRGINVQPISFTGRTPREERERMRITIPDFVKLVEEQTDGRIPAESFYPVPIAAKIARLIGQLHGKRKPEFSAHPICGVATYLLDEGDWYRPITDYVDPDAFIDALEEVAEKAGSLDRKRDRAKAAWIVTKYLRRVFKGLTGKRKLARLVLDVVTKGTYDALADFHWNALLLGCMHFMDPYNFQTDRVRRCVIHYATPDGRIVPFCPYNSIHREEIERKFGVPLEEWKERRNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ1</accession>
    <name>Q8TWJ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Terpene cyclase/mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02256">
        <property type="protein sequence ID" value="AAM02256"/>
        <property type="gene ID" value="MK1043"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1043"/>
    <dbReference type="HOGENOM" id="CLU_982157_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="PFTB_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="283" mass="31703" checksum="E1F3A52627EBEBB1" modified="2002-06-01" version="1">MRFYFAVDKARERGVPIKEFKLIQEDGSVYWEGKFGRSKWETSGEVIYGNMVALSGIYLGLLSGEVKPKTWPEPERSRFEKGITVGLARILSLQQEDGGWGWKVVLVEGKTRFPAGKGHVLYTAKILAKVLIPALRLNIKNVSYGGTTYDVAEHARAAVRFLIDQQLESGGYSANKEWSMTEDPLYTAWALRALCEAYRYRDLLGLDDATVQQVKEAIRRAVDWLLSHQEDEGDYAGLWEMKSAAIMGSAYAPPSEAQIVRALIQAYSISEELGLNKAELKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK1</accession>
    <name>Q8TWK1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">radA_1</name>
        <name evidence="5" type="ordered locus">MK1032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1032"/>
    <dbReference type="EnsemblBacteria" id="AAM02245">
        <property type="protein sequence ID" value="AAM02245"/>
        <property type="gene ID" value="MK1032"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1032"/>
    <dbReference type="HOGENOM" id="CLU_041732_0_0_2"/>
    <dbReference type="OMA" id="REGFIYE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01123">
        <property type="entry name" value="Rad51_DMC1_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033925">
        <property type="entry name" value="Rad51_DMC1_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RECA_2" evidence="4">
        <location>
            <begin position="85"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="316" mass="35210" checksum="1811E2BFDF421D32" modified="2002-06-01" version="1">MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIADAREEASKGIMEAKTLADLLEEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGKSQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKKDELKEVSDRFVLRRIIDAAALRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYCTAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDKPPFEVQVELTDRGLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK6</accession>
    <name>Q8TWK6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyruvate-formate lyase-activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1027</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1027"/>
    <dbReference type="EnsemblBacteria" id="AAM02240">
        <property type="protein sequence ID" value="AAM02240"/>
        <property type="gene ID" value="MK1027"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1027"/>
    <dbReference type="HOGENOM" id="CLU_755678_0_0_2"/>
    <dbReference type="OMA" id="ILAYFPE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="35"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="316" mass="35868" checksum="475FF6786242CDFA" modified="2002-06-01" version="1">MAELGYEKAERCEVGEKPACKVGLRDGKRLIHDAHLSRPEHYYSVYQSCCNWECLFCHSWRFTQRPVGTWWSPEDFVRHALEYRETVTVWEPRSRATSFHATDLCLGCGRCVTLGERPEWCPGELDPDQVVPSPQGWGPARNIVAFTGGDIACRPEFYVESIRGLKSETDDLWVLLETNGFGLVRENVEELVAAGLDAVWLDVKAWKEDVHRKLTGATNRYTLKAIELLVEHDVVLEVCTLYIPGYVEADQILRIAKYVADIDRNIPFTILAYFPEYKLSVRPPKRSELETAERLAREVAGLKNVKIGNEGVALPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM1</accession>
    <name>Q8TWM1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">V-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ntpI</name>
        <name evidence="11" type="ordered locus">MK1012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1012"/>
    <dbReference type="EnsemblBacteria" id="AAM02225">
        <property type="protein sequence ID" value="AAM02225"/>
        <property type="gene ID" value="MK1012"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1012"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1062"/>
    <dbReference type="HOGENOM" id="CLU_025558_1_0_2"/>
    <dbReference type="OMA" id="MAVNIMA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="PTHR11629"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="567"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="591"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="237"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM02225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="656" mass="73475" checksum="D841A4DBFC9C36E4" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ6</accession>
    <name>Q8TWQ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0976</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0976"/>
    <dbReference type="EnsemblBacteria" id="AAM02189">
        <property type="protein sequence ID" value="AAM02189"/>
        <property type="gene ID" value="MK0976"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0976"/>
    <dbReference type="HOGENOM" id="CLU_454659_0_0_2"/>
    <dbReference type="OMA" id="NIADREV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019204">
        <property type="entry name" value="DUF2070_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09843">
        <property type="entry name" value="DUF2070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="577"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2070" evidence="3">
        <location>
            <begin position="49"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="600" mass="65017" checksum="CE015980C3D7C773" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR2</accession>
    <name>G3PP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyceraldehyde 3-phosphate phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0970</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02183" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02183.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWR2"/>
    <dbReference type="SMR" id="Q8TWR2"/>
    <dbReference type="STRING" id="190192.MK0970"/>
    <dbReference type="PRIDE" id="Q8TWR2"/>
    <dbReference type="EnsemblBacteria" id="AAM02183">
        <property type="protein sequence ID" value="AAM02183"/>
        <property type="gene ID" value="MK0970"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0970"/>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="OMA" id="TYHNVKF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107330" description="Glyceraldehyde 3-phosphate phosphatase">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q58832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="233" mass="26692" checksum="D73940D03DA38818" modified="2003-10-31" version="2">MIKAVLFDVDDTLYPSSKLAEEARRNAIRAMIEAGLETDLSEEELYRELQEVVKEYGSNHPRHFDLLLRRIGADPEPKLVAAAVVAYHDTKFAYLKPYPDVIPTLMQLREMGFKLGAVTSGLAVKQWEKLIRLGIHHFFHEVVISEEIGVEKPNPKIFIEAARRLGVKPEEAVYVGDRLDKDIRGANRAGMVTVRIRRGKYQDMEPRNDDDVPDFEIDRPRELLDVVRELAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS3</accession>
    <name>Q8TWS3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0959</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02172">
        <property type="protein sequence ID" value="AAM02172"/>
        <property type="gene ID" value="MK0959"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0959"/>
    <dbReference type="HOGENOM" id="CLU_1976531_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="126" mass="14278" checksum="B63D0671DAFACF6F" modified="2002-06-01" version="1">MDPLTEDDVELREITLMVPIAEANLISNHKFRNRKRDAAYASYLVGSSSTDVDDVIPVTVRIPWPKDVKAPREMPLVPGTLTHHRRFCFTVPNPPETRKLSEKTLVTAALLRTLSHELDLTSAIRY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-10-25" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS7</accession>
    <name>NAC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nac</name>
        <name type="synonym">egd2</name>
        <name type="ordered locus">MK0955</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWS7"/>
    <dbReference type="SMR" id="Q8TWS7"/>
    <dbReference type="STRING" id="190192.MK0955"/>
    <dbReference type="EnsemblBacteria" id="AAM02168">
        <property type="protein sequence ID" value="AAM02168"/>
        <property type="gene ID" value="MK0955"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0955"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1000"/>
    <dbReference type="HOGENOM" id="CLU_146475_0_0_2"/>
    <dbReference type="OMA" id="PRKMKQM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="HYPK_UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00264">
        <property type="entry name" value="TIGR00264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000135603" description="Nascent polypeptide-associated complex protein">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="NAC-A/B" evidence="1">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <sequence length="116" mass="13216" checksum="A836CB4E0134E046" modified="2002-06-01" version="1">MFPGGKIDPRKLQRLMREMGMEQEPISGVERVEIHLKDGSKLVFEKPQVIRMKIMNQEFYQVAGKAKREKPEEEPFTDEDVKLVAEQAGVSEEEARKALEETGGDLAEAIMRLQGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU0</accession>
    <name>ASSY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">MK0942</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWU0"/>
    <dbReference type="SMR" id="Q8TWU0"/>
    <dbReference type="STRING" id="190192.MK0942"/>
    <dbReference type="EnsemblBacteria" id="AAM02155">
        <property type="protein sequence ID" value="AAM02155"/>
        <property type="gene ID" value="MK0942"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0942"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.987"/>
    <dbReference type="HOGENOM" id="CLU_032784_4_0_2"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148677" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="394" mass="44475" checksum="064687A6DD47301D" modified="2002-06-01" version="1">MSRVVLAYSGGLDTSVCIELLRERYGYDEVITVTADVGQPEEELREAEEKARKLADEHFTVDCVDEFVCEYCWRAVKANATYEGYPLSTALARPLIAQKVLEVARDVDADAVAHGCTGKGNDQFRFESYLRAHGGEEFEIVAPVRDLNLTRDEEIAYAEERGIPVPVDVDSPYSVDENLWGRSIEGGVLEDPSEEPPEEVFEWTVSPAEAPDEPEVVEIEFEDGVPVEVDGRDDPVEIVRYLNETAGEHGVGRIDIIEDRVIGLKSREVYEAPAAVTLLEAHRALEAFTLTRRELSLKASLEEEWARLVYDGLWFNPLREHLEAFFDSTQRHVEGTVRVKLFKGSATVISRESPRALYEEELISYEEKQFDQRTAEGAVKLHGLQERLALRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV5</accession>
    <name>Q8TWV5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylmuramyl tripeptide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">murE_1</name>
        <name evidence="2" type="ordered locus">MK0926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0926"/>
    <dbReference type="EnsemblBacteria" id="AAM02139">
        <property type="protein sequence ID" value="AAM02139"/>
        <property type="gene ID" value="MK0926"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0926"/>
    <dbReference type="HOGENOM" id="CLU_574423_0_0_2"/>
    <dbReference type="OMA" id="FEYGIRD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="1">
        <location>
            <begin position="57"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="476" mass="52842" checksum="935599A30731CB1E" modified="2002-06-01" version="1">MSLRSKLADLAGLLALKAVRFGPGMGRSFPGWTVIKVGGFDAVRELARRPEYGVVLITGTNGKTTTTRLACALLAEDAEVSCNYDSNTINAVTTGLLKGRKAELVVTEYGIRSREYGIPDTVCELVDPLAIAYTTISKEHFRENADKKDPFGAYFEAKRLLARPLKDGVLVLNADDPRVTYIRDEKRGDPVEYVFYGLEVEIEDLTPPTEELECPACGGELDYETRYFNHKGLYSCRDCEFSRPDPDVAVVRLKGGPDEWEVTLSYDVTNAVIGERLEGKVTYELPLPGLHNVYNSVCAISLYLAVTPRPEDPEGTIRRVIEGLNPLEFIPSGRFEVLNVGGKPVGVGQGDNGDAFKANANLMLSVAGDVGACVYTTPDEGEHPIFEMHRIILRALEPDELYVFPGRESVKAAEEYYEELTEEFDAEFYPIPHERMQEKVEEMRRVCEEADGPVFASGCGPEREMWEALKRELRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX30</accession>
    <name>Q8TX30_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane protein implicated in regulation of membrane protease activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0848"/>
    <dbReference type="EnsemblBacteria" id="AAM02061">
        <property type="protein sequence ID" value="AAM02061"/>
        <property type="gene ID" value="MK0848"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0848"/>
    <dbReference type="HOGENOM" id="CLU_1811447_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="3">
        <location>
            <begin position="58"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="142" mass="15553" checksum="34AE784117796477" modified="2002-06-01" version="1">MDTLSQELCLLLAGVGLIVLDCVLNTGIVAMIGVVCAWVGAYLMGTKYLIATLPPGAFVLLMQVAAVLRRTEESSDVLLKSVGELKGEEGIVIREDPLLVKVRGEPWRAVSKTRLRRGERVVVLDVKGNKLVVKRRKTGERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX55</accession>
    <name>Q8TX55_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsZ</name>
        <name evidence="10" type="synonym">ftsZ_1</name>
        <name evidence="10" type="ordered locus">MK0821</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0821"/>
    <dbReference type="EnsemblBacteria" id="AAM02034">
        <property type="protein sequence ID" value="AAM02034"/>
        <property type="gene ID" value="MK0821"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0821"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.863"/>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 6 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="8">
        <location>
            <begin position="43"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="9">
        <location>
            <begin position="236"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="360"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="363"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="51"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="138"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM02034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="407" mass="43041" checksum="086C1AFE0AD3F927" modified="2002-06-01" version="1">MEQIVKNALERVEREQGGDDPPSSRGTDDDQELMEVLERARARILVVGVGGAGNNTATRLKEEGIGGAEVIAINTDAQDLVSCKADRKVLIGYELTRGLGAGGDPRVGEEAAKEDMEKIKEVVEGADMVFVTCGLGGGTGTGAAPIIAEVARKEGALTIGVVTLPFSVEGRRRIENALEGLERLRQVADTCIVIPNDRLLEIVPDLPIAAAFKVADEVLINAVKGITEMITQPGLMNLDFADVRAVMENGGFALIGIGEAENDSESGSRAVQAVENALNNPLVDVEVSGATGALVNIVGGKDLTLKEAEEVVELVASELSEDATVIWGAQIDEDLNDVLRVTVIVTGIEDADLEAMFTGPRQPKRTEVKEVAAESSRPEKVPEVSKGGAESSEDERPSSLEDLEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX88</accession>
    <name>Q8TX88_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein containing cytidylyltransferase domain and predicted nucleotidyltransferase (HIG superfamily) domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0787</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0787"/>
    <dbReference type="EnsemblBacteria" id="AAM02001">
        <property type="protein sequence ID" value="AAM02001"/>
        <property type="gene ID" value="MK0787"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0787"/>
    <dbReference type="HOGENOM" id="CLU_051457_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CTP_transf_like" evidence="2">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="427" mass="48648" checksum="1D4BDE0715B86749" modified="2002-06-01" version="1">MTVIGIAADFDPPHRGHAYLLDRARDLGDEVVVFLNADYTAHHTPPLLPYRLRREIVLELGADEVIPVRGYHQRFPLAYTVPVRVRLMAEEGVDVILDAGPSRNLDRLREHVERVLEVSDLFSIPPNVPARNVVRWLAAVEYVNRELGTDVELLLIPELEGYSGRKIRAALRRSGYSPDSLRKVRRHLPRETFKKLERYLKARTPPIARRKELLDVLNRASSYELCSIAHLNTIAVREILRGRPFRSERQVWGALRRADYGSVLTRLALANTECRVTSDEIARIALSWCVERLVPENQSPDSMYRRDWFVAALSSHGVKARDADRLYKRADSYEEARRLARRRYGVDPGVPRFETLIGEARVSDGEYRPAISANGRLGVVEDGFQELRATAFGATLLRYVLDDPFVDAVIRVEDEEARLIVFPGDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA9</accession>
    <name>Q8TXA9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01979">
        <property type="protein sequence ID" value="AAM01979"/>
        <property type="gene ID" value="MK0765"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0765"/>
    <dbReference type="HOGENOM" id="CLU_2115498_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="114" mass="12313" checksum="03B0A43662F2CE4F" modified="2002-06-01" version="1">MSELMVDVHLLSHKPVPERRLEKEVSEALRRAGFDVLSVRATRGSDIVGAFRAVEYVILVDVRGEGDPEGPVKRALRDYGRVEVQVGSRSGSGECDEEALEALFEGSKGADSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC3</accession>
    <name>Q8TXC3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0751"/>
    <dbReference type="EnsemblBacteria" id="AAM01965">
        <property type="protein sequence ID" value="AAM01965"/>
        <property type="gene ID" value="MK0751"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0751"/>
    <dbReference type="HOGENOM" id="CLU_1773225_0_0_2"/>
    <dbReference type="OMA" id="RPGSYIK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="149" mass="16673" checksum="CF14262A184ADBA2" modified="2002-06-01" version="1">MEEERKRKWIKPWPVSRRNLAIMLMGALLLSIGRIAEYTAWAARPVPKQEGLRIIGIDVVGCDFLPEEVIRSNVMASGLRPGSVIKGGTLITPEGKKIPLHEAIRRARIYAMRSVIPGTKIKPIRSVQITIKRSGVVVVRVIEDYGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC9</accession>
    <name>Q8TXC9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metalloprotease fused to aspartyl protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01959">
        <property type="protein sequence ID" value="AAM01959"/>
        <property type="gene ID" value="MK0745"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0745"/>
    <dbReference type="HOGENOM" id="CLU_978639_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008503">
        <property type="entry name" value="Put_Zn_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05618">
        <property type="entry name" value="Zn_protease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="SSF50630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn_protease" evidence="1">
        <location>
            <begin position="46"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Zn_protease" evidence="1">
        <location>
            <begin position="128"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="284" mass="31297" checksum="E06012008C2393FD" modified="2002-06-01" version="1">METVGVTTVVTVSGLTGSKRVAARVDTGAENDSIDLKLASEIGAGPVIGVKKVRSASASRLRSERRPVVHVTLELAGRCLPSEATLADRRDMRYPMIVGRKTLRRAGVTVDPSREEEPEGDEVDPRRVVGTLKLHKRLLRTVGEKRAVTPAVLALQHGGAWSYRDGDVSSVAVPIRKLSNGVVREDLYLLLNPEIERAEGTLTRLEKCGRERVRRVAKRPRRLEVRHDGGAIIRVDPGRRRIRVRELDPGTLRLEGIPAANLHHELSHLMGDDLGPSVLEFEVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD2</accession>
    <name>NADK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD kinase</fullName>
            <ecNumber evidence="1">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadK</name>
        <name type="ordered locus">MK0742</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAM01956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXD2"/>
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    <dbReference type="STRING" id="190192.MK0742"/>
    <dbReference type="EnsemblBacteria" id="AAM01956">
        <property type="protein sequence ID" value="AAM01956"/>
        <property type="gene ID" value="MK0742"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0742"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.782"/>
    <dbReference type="HOGENOM" id="CLU_008831_0_3_2"/>
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    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:NAD metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:NADP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
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        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
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        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120701" description="NAD kinase">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="179"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <sequence length="276" mass="30665" checksum="CD0A6C3606133CE2" modified="2002-06-01" version="1">MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVDMIITIGGDGTILRVSRITSEYEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEEHRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGFELETTWADGVLVATPTGSTAYSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYMNLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG0</accession>
    <name>Q8TXG0_METKA</name>
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        <submittedName>
            <fullName evidence="7">Transcription elongation factor NusA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA_2</name>
        <name evidence="7" type="ordered locus">MK0714</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
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        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH_5" evidence="6">
        <location>
            <begin position="67"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="180" mass="20105" checksum="C3ADBC195E1C70C6" modified="2002-06-01" version="1">MILGGLKLELPICEVCAKTGLLCPGCEERLEQGEITETDVEVSKKLVELKEDHPSLEDVALLRTIDTGNLVVLVTKQGMAGKLIGKRGRISRALSDHLGKKVRVVEEVEDPKDVRQLRKLIQDLILPARLLSVNVVYEPDGERYKAVIHHRDRHRVPADTEELEKAVKELTGMEVEVTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH3</accession>
    <name>Q8TXH3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted secreted protein specific for M.kandleri, MK-18 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_2893259_0_0_2"/>
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="62" mass="6563" checksum="6A368794C05DA915" modified="2002-06-01" version="1">MSGQANIELLVLGVLVAAVAIMAAVIGYYMPLKKSSSGLGDVARAQNEKISNVCHELATKWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI0</accession>
    <name>Q8TXI0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01908">
        <property type="protein sequence ID" value="AAM01908"/>
        <property type="gene ID" value="MK0694"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0694"/>
    <dbReference type="HOGENOM" id="CLU_1682705_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="156" mass="17242" checksum="F2ECF554F4B4D022" modified="2002-06-01" version="1">MGARTFGQVSVEFVLLSIIVLTAAFIAGRYMGPEYEWYCVKKAVSDAVAKTREQVAVGVTYRVGDQEVEISGNLSVVRCQVNDDRIEYELKYSGDNPAGVVDLLRRNVLLQVYVALYGRPKSVSEISNPVKGSWANYQVEVSGGESIKVVVERTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI9</accession>
    <name>Q8TXI9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase largest subunit, the N-terminal part</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpoC_1</name>
        <name evidence="3" type="ordered locus">MK0684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0684"/>
    <dbReference type="EnsemblBacteria" id="AAM01899">
        <property type="protein sequence ID" value="AAM01899"/>
        <property type="gene ID" value="MK0684"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0684"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.723"/>
    <dbReference type="HOGENOM" id="CLU_037097_1_0_2"/>
    <dbReference type="OMA" id="TPMMTVY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA_pol_Rpb1_5" evidence="2">
        <location>
            <begin position="35"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="48034" checksum="E5554D5060F7EA50" modified="2002-06-01" version="1">MNVEELLEEVCKEEDVELPDSVHREIVEKVSEDEELKDADEETVRKVLRALVRNYKCRQIERGDAVGVLAAQSMCEPATQMTMRTFHLAGVAEIDITTGMPALERVLSLPYHGPPTWVIELRLKEPYRSDREKAEGVARKIEETRFEELTEYIEVNPDECAIEIGLDEERIEEHGIDEDQLVAFVREHTGGACHLEGDVLKVTLADVESASELAEIANDLREAVVKGIEGVLHASVQYDEDEEEYYILVRGSPEGAPGRPPRWISQRARNSKVAKRAYLMLQLMQLDEVDATRVNTNDPKIVDVLLGVEAGRNAIIEQLEEVYESQGISVDPRHFMLISDMMSFWGELRSVRSVGGYSVMELKGSPLTKAAFEYVSRVLTETATSGDVEELRGVLENIIVGKPVPVGTGTVELTVDYSKFKEGEEEGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ6</accession>
    <name>Q8TXJ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01892">
        <property type="protein sequence ID" value="AAM01892"/>
        <property type="gene ID" value="MK0677"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0677"/>
    <dbReference type="HOGENOM" id="CLU_3039055_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="54" mass="6066" checksum="36ECA262C13C52B8" modified="2002-06-01" version="1">MVKSSHVPIVSPNDPAVVCPNCTKEEYKRAKRERGKLRFAGLCEECGSAAFYEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL7</accession>
    <name>Q8TXL7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01860">
        <property type="protein sequence ID" value="AAM01860"/>
        <property type="gene ID" value="MK0645"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0645"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.684"/>
    <dbReference type="HOGENOM" id="CLU_791345_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="350" mass="38111" checksum="0EF1A1BBB581CF6D" modified="2002-06-01" version="1">MGRDEGEDKFDGGDEGTSDEPDVPEIRLDGESDGPDHDHPEGPDSDSEPQYPQNILSHEGDHTDIDVSNTNISTAESDLEADLDSNSYSESSSESDATQYTAQYSDLDQDTDQIQDSRSESISDVESVQESSSESNPDVDVNVDNVVDVDDPADPGVIYDVDREVNVDDRDTYVEENVYYGDTDRTIYDPRTDSDTGSVDPSSDPTTAGDQVVVGSEDPYMVLDTQKGQVDVLVGEDPTTINMGQSELSQLLDLVNWLDGNAPSDVLDLLYQLLAYLLETFYGLIPLDNILNMIQQIAEYALSGELGLDVLNQLLGVLETATSPLYAATEILKFLGDLDLGLDALLDIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN0</accession>
    <name>Q8TXN0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted metabolic regulator containing two V4R domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0632</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01847">
        <property type="protein sequence ID" value="AAM01847"/>
        <property type="gene ID" value="MK0632"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0632"/>
    <dbReference type="HOGENOM" id="CLU_955148_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="2">
        <location>
            <begin position="223"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="291" mass="31796" checksum="1A8B5CB893FB606F" modified="2002-06-01" version="1">MPGCGIGHGDDPTQRSGRDEPARHVGADGSNREAGCLLARELGDDLESTLTTFADLTGCEVDADEDVVTVRNYPECAGYPGARGPVCHFMRGLIAGAHELETGERTPVEEVRCRAAGDDVCEFRIGRKPREPTVPRLDGLDERALRRMAHAASEREPEEVAAFKLASEYTLKFHPADAAAPIFFRAGRLYARALLRLYNPLEMTSFLSIVEDVTGAECHLGEGNRMSIEPCPECAGFPREHEPVCHGVRGALHETLGTFGKEVERVEETECAAVTGELVGRCEFRFELRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN3</accession>
    <name>Q8TXN3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing V4R domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01844">
        <property type="protein sequence ID" value="AAM01844"/>
        <property type="gene ID" value="MK0629"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0629"/>
    <dbReference type="HOGENOM" id="CLU_1763881_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="78"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="147" mass="16563" checksum="EB1028251870F5B2" modified="2002-06-01" version="1">MTEIARAATRYEPEETAALRIATWYLVRRSLDFEAPATLFWAGRTYARALLRVYHVLSIREFLTCIERVLGTSYDLNEDVVVAAPCPECAGFPRGYESVCDATRGFIHETLAAFGREPHKVREAKCAVRAGELVNACAFEIRHSNLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP9</accession>
    <name>Q8TXP9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0611"/>
    <dbReference type="EnsemblBacteria" id="AAM01826">
        <property type="protein sequence ID" value="AAM01826"/>
        <property type="gene ID" value="MK0611"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0611"/>
    <dbReference type="HOGENOM" id="CLU_072733_0_0_2"/>
    <dbReference type="OMA" id="FTHTYAG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="338" mass="39589" checksum="C5FAA2F78C7C4C9F" modified="2002-06-01" version="1">MYPMLRDFLVTEDAVFSVISYVHPEDGFLALMRYVKDPEGDRIRRPTGERYRKVSFEESFEILRERHPEYVRKVRGDFYDQVVPPEDVVEHLHPRDRMERILEEPRDDLEEMAARAVLELAEDSGVPESRFGVTGSLLPELHDPAESDLDLVVYGVQEFLAVRDAILRIQETGEGRDLLRALDYDQWERVYRRRSPELDFEEFLRHELRKGNRVVIGDRVCDVLLVRDESELSDVRWEEFETVEENVRLVCEVLDDSLAFDYPARWRVEAVESDTEEGYEVTEVRSYTHTYVGQAFEGEVIEVSGKLERERNSGELIAIVGSSREAEGEWIRVLKDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR1</accession>
    <name>Q8TXR1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0599</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0599"/>
    <dbReference type="EnsemblBacteria" id="AAM01814">
        <property type="protein sequence ID" value="AAM01814"/>
        <property type="gene ID" value="MK0599"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0599"/>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="OMA" id="HYYDIQH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_TRM5_TYW2" evidence="3">
        <location>
            <begin position="93"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="38755" checksum="A21A7B5B179A443D" modified="2002-06-01" version="1">MPELPYVAVPKHMAERARRVLSREDLLAKDVKARREEDMVLFPVRDAGRAGSVLEELGIPYEAGEAEFERTVEHRSVEEVLEDSFGWSVPVPYDVIGDVAVVQIPDELRGHEREVGRAIMKVHRRVRAVFERGPVRGVFRVRDLRRIAGKGPAVTEHREHRCRFRVDLARCYFNPRLATERRLLAEDVVEEGSTVLDLFAGVGPITVILKRFVPSVEVTACELNPVAYRYLLENLRLNRVEARAFLGDAREVSRLVGRFDYVIMNVPKMAHRFVETAVRCVRPEGRLIYYRIAPNAEEAFEELRRKADRPVELESHREVKPYSPEESLYRLVVRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX6</accession>
    <name>Q8TXX6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metal-dependent hydrolase of the TIM-barrel fold</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01748">
        <property type="protein sequence ID" value="AAM01748"/>
        <property type="gene ID" value="MK0533"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0533"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.568"/>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="OMA" id="HPMGYPK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="256" mass="28241" checksum="0561CDE4877AF522" modified="2002-06-01" version="1">MRYVDAHVHLDVRSYEDLERMALSGVRTVVTCAHDPYPDMTAEVYSALFRRLLGVETWRGEKAGLTVKVAVGVHPGGVPDNMSDVLREVEELLSHEDVVAIGETGLNENPDDREIQVLEAQLKLAREHEVPIIVHTPSRNKVEITEKVLEILNSSGIDPSLVLVDHASAETVSLIGEEGYAVGLTLRPGELDVWEACDIVEEYADEMTLIASSDLGSLAADPLALPKLALELERRNVEKSIIRDVVARNAERFYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ0</accession>
    <name>Q8TXZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0518"/>
    <dbReference type="EnsemblBacteria" id="AAM01733">
        <property type="protein sequence ID" value="AAM01733"/>
        <property type="gene ID" value="MK0518"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0518"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.551"/>
    <dbReference type="HOGENOM" id="CLU_062565_0_0_2"/>
    <dbReference type="OMA" id="YGVPLYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009995">
        <property type="entry name" value="DUF1512"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07431">
        <property type="entry name" value="DUF1512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016495">
        <property type="entry name" value="UCP016495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="59"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="372" mass="41007" checksum="D56FC99B43661A4C" modified="2002-06-01" version="1">MLALNVDTSLVENLVYFIVSIILFWLFIRVYYQVVLFRRIEGEVERIRKIDEKVQQMVVRRARCDREEAETLVQRAANLFVVQPVQLDPHGIIERLENLIERSEDKFRSYARVLTGKRDGVEVDNMSMALTCVFSIHVIAQYLRHLLLSAKKTNNIQLLLLITMLLPTFKQFVKSNYEGAKAFLKCVPIGDSVGPMVAARLIGDSPVREVEKGTVAAEKELEGRRLLIVKAHGPGGNLGRLGRAVKKLVREYGDVSKIITIDAALKLEGEKTGKVSEGVGVAMGDPGHESYKIEQIAADEGIDLDAVAIKMSATEAVMPMPKSVVDAVEEAAERALELAKEAPEDSTVIIVGVGNTVGIPDNRPYVSNGGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY55</accession>
    <name>Q8TY55_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATPase of the HSP70 class involved in cell division</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ftsA</name>
        <name evidence="3" type="ordered locus">MK0451</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TY55"/>
    <dbReference type="EnsemblBacteria" id="AAM01666">
        <property type="protein sequence ID" value="AAM01666"/>
        <property type="gene ID" value="MK0451"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0451"/>
    <dbReference type="HOGENOM" id="CLU_503104_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="541" mass="58946" checksum="C5A9C9CA625BE9A3" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY70</accession>
    <name>DAPH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tetrahydrodipicolinate N-acetyltransferase</fullName>
            <shortName evidence="1">THP acetyltransferase</shortName>
            <shortName evidence="1">Tetrahydropicolinate acetylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapH</name>
        <name type="ordered locus">MK0435</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of an acetyl group from acetyl-CoA to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + acetyl-CoA + H2O = CoA + L-2-acetamido-6-oxoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:13085"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58117"/>
            <dbReference type="EC" id="2.3.1.89"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (acetylase route): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family. DapH subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.89" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY70"/>
    <dbReference type="SMR" id="Q8TY70"/>
    <dbReference type="EnsemblBacteria" id="AAM01650">
        <property type="protein sequence ID" value="AAM01650"/>
        <property type="gene ID" value="MK0435"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0435"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.464"/>
    <dbReference type="HOGENOM" id="CLU_103751_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00022"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047200">
        <property type="term" value="F:tetrahydrodipicolinate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01691">
        <property type="entry name" value="DapH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019873">
        <property type="entry name" value="DapH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013710">
        <property type="entry name" value="DapH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08503">
        <property type="entry name" value="DapH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03532">
        <property type="entry name" value="DapD_Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000376733" description="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01691"/>
        </source>
    </evidence>
    <sequence length="245" mass="26275" checksum="3DEADC8BC1E6A79F" modified="2002-06-01" version="1">MRPLSELDPEELIRYISESPKRTIAKFYVKTDDPEGLAERLEERLEDAKVFTGVDHVIVIGEHDDVVEVLESEDSVEYYHKELDHRNRAVPLADYSEFEDVRIEPGAIIREKVKLGKGVVVMMGAVINIGAKIGDGTMVDMNAVVGSRAEVGKNVHIGAGAVIAGVLEPPSAKPVVIEDDVVIGANAVILEGVRVGKGAVVAAGAVVTEDVPPSKVVAGVPARVVKDVDKKTEAKTQIVDALRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC6</accession>
    <name>Q8TYC6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01591">
        <property type="protein sequence ID" value="AAM01591"/>
        <property type="gene ID" value="MK0376"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0376"/>
    <dbReference type="HOGENOM" id="CLU_170076_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="83" mass="9289" checksum="E497F106F8063C2E" modified="2002-06-01" version="1">MHALRCEAELKSERVARVVYESVLPDVKLMPARRSRVDIDRRGNNIQIEIRAEDVSALRASASGVFRLLALSERVITTVLGDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC7</accession>
    <name>PFDB_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdB</name>
        <name type="ordered locus">MK0375</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC7"/>
    <dbReference type="SMR" id="Q8TYC7"/>
    <dbReference type="STRING" id="190192.MK0375"/>
    <dbReference type="EnsemblBacteria" id="AAM01590">
        <property type="protein sequence ID" value="AAM01590"/>
        <property type="gene ID" value="MK0375"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0375"/>
    <dbReference type="HOGENOM" id="CLU_131909_0_1_2"/>
    <dbReference type="OMA" id="PPQVQAM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124861" description="Prefoldin subunit beta">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="120" mass="13749" checksum="C62AF31EF238AAD3" modified="2002-06-01" version="1">MAQNVEQQVAQLQQLQQQLSSIVAQKQQLELQLREIERALKELDEIEEDTKVYKTVGGLLIEADRDEVKEELEDRKETLELRVKTLEKQEKRLQQQIENLQKRLQKALQQAEGGGGAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF4</accession>
    <name>Q8TYF4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0346</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01561">
        <property type="protein sequence ID" value="AAM01561"/>
        <property type="gene ID" value="MK0346"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0346"/>
    <dbReference type="HOGENOM" id="CLU_071768_0_0_2"/>
    <dbReference type="OMA" id="DCEGPLT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024196">
        <property type="entry name" value="NiFe_hyd_3_EhaR"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019370">
        <property type="entry name" value="EhaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="326" mass="36545" checksum="96FB491A064B1CFD" modified="2002-06-01" version="1">MPVVFDLEGPLSPMDHAYEVMARCVPNGREIFEVISFYDDVVYFEEQREGYEPGTTLALIVPFLVVHGVTEDDLRRVSEDATLTPGARETMRELDATPFVASTSYEQHAMRIAGLVGIPRGHVYCTEMPLDDAPEPSEEERRLVLEMEREILRKFYPPSDADKEELVESVDDFFGRLSETELGEFCSKIEVVGGSRKARVVEEVVDLEDADVSEIACVGDSITDVDMLKFVRKRGGLAVAFNGNEYCIPEADVAVAAPSLSAVLPILETFFEEGKDEAMELAEEFEPEGEARVLNVNRARQRELEEFIRYSEAMRERVRGEAGKLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF7</accession>
    <name>SYW_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="1">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpS</name>
        <name type="ordered locus">MK0343</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYF7"/>
    <dbReference type="SMR" id="Q8TYF7"/>
    <dbReference type="STRING" id="190192.MK0343"/>
    <dbReference type="EnsemblBacteria" id="AAM01558">
        <property type="protein sequence ID" value="AAM01558"/>
        <property type="gene ID" value="MK0343"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0343"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.364"/>
    <dbReference type="HOGENOM" id="CLU_032621_3_0_2"/>
    <dbReference type="OMA" id="SIYHRFM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136724" description="Tryptophan--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="71"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="247"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <sequence length="374" mass="42733" checksum="0BD4E5279321B828" modified="2002-06-01" version="1">MIDPWDVEEVDYERLTEEFGIRPIDEKVRELLPRRFPLLDRGIVFGHRDYDSFLKDYNDGKLVSVLSGMMPSGRMHLGHKTVVDQLVFYQQEMDVKVYVPIADLEAHHARNMDLDRAHRIAVEEYVLNYAALGLDLDPDRCEIYLQSERKTVQRMALLLAGRLTWNTVKNTYGFTGETNMGHAFAPIVQAADILHPQEIEGPHRVLVPVGVDQDPHLRLTRDIAEKEDLIKPASTYHRFMTGLTGGKMSSSKPNTAIFLTDDPETAKEKVWNAKTGGGATLEEHREHGGNPDECVVYELMVYHLADRIGGDEKLREIRKKCREGDIICGECKRMVGEALAEILEELERRREDVRDELPDLLSQHPDAPEVPEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH1</accession>
    <name>Q8TYH1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01543">
        <property type="protein sequence ID" value="AAM01543"/>
        <property type="gene ID" value="MK0328"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0328"/>
    <dbReference type="HOGENOM" id="CLU_1425096_0_0_2"/>
    <dbReference type="OMA" id="WFYRNWK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="190" mass="21425" checksum="412BF7881231E24B" modified="2002-06-01" version="1">MSDDKRSYARPLGRTAMFVVASGGYYQLYWFYRNWKDFKEYLGRDVNVVLRTIGLFVPFVNLYLVWKQFVEINELLEEAGLDPCPTKILYPVWFGFFFAGNRGIFHAETRVDALGAIVALMVSCVPLLVVQKKLNEYWREVQGGLTPTWSVQGRADGGGGVVPADMGRVADLGVNARSRRTDSGEPRAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI5</accession>
    <name>Q8TYI5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-42 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0312</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01529">
        <property type="protein sequence ID" value="AAM01529"/>
        <property type="gene ID" value="MK0312"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0312"/>
    <dbReference type="HOGENOM" id="CLU_1163806_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="26479" checksum="886D1C9F618D1634" modified="2002-06-01" version="1">MTTQQNGKPITLQPFTIPRLPFRARRPHRHGLRTVPHALHPARARSHARLHPRRDRGPLSGQLLIARRVLFPYPPVPTTLQAEIERPTNYRLNGKQVSFWVPAPDVKYEVRSMVKANGQLSFQHVQGVSERQGIVTVELQGDPEFVGVLLRQPPRVAYGVRDRVRLSLRGRGPAARGVKVSWRADGERTRIELSGSGRVLLGLILDGGAMDVRVDGGSVLETGPCRAGGVTRSWTWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI7</accession>
    <name>Q8TYI7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Orphan DOD family homing endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0310"/>
    <dbReference type="EnsemblBacteria" id="AAM01527">
        <property type="protein sequence ID" value="AAM01527"/>
        <property type="gene ID" value="MK0310"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0310"/>
    <dbReference type="HOGENOM" id="CLU_663265_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="2">
        <location>
            <begin position="194"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="414" mass="45528" checksum="4D56714A52A2BA1E" modified="2002-06-01" version="1">MSPTSSSGSASPSAPDARPDRGFPTPFTASRPTSRPPTSPVSSTPGPPSPTAPPTTPRVTLTMTSPARHRDGLAAFMRDVLDLLVTLGVHAVATGPSGREYLRTHLHIDGDTHVHGFLTDVGFPYHTRKRREAHRLGTRLDHRIERLRAEPVSDPPEPVLRDPGLPDRQGTYLRRRGLLPIALDDHKIPLLARVLGYALGDGSLHEIDGQLRLTFAGEPEGLRDLADDLATLGVGTGLRRWDTKDMVELHVHSRAFARYLELLGMPRSPKVERVYGVPGWILRAPPGVKAEFLAGLFGADGWLTGSDGRYAIGLTQAKSAVYLGSLHLFMTEIGEMLEEVAGCRWGLNRERCYTTEDGKVRVTERLFICGREDVMRFLSDVGFAYSRAKAERTERALKALGGSVRGIRLKYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL0</accession>
    <name>Q8TYL0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pyruvate-formate lyase-activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0286</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0286"/>
    <dbReference type="EnsemblBacteria" id="AAM01503">
        <property type="protein sequence ID" value="AAM01503"/>
        <property type="gene ID" value="MK0286"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0286"/>
    <dbReference type="HOGENOM" id="CLU_044176_0_0_2"/>
    <dbReference type="OMA" id="TCVCFFG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="122"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="39629" checksum="BD357632A090F312" modified="2002-06-01" version="1">MTCELCGDPASSFLRVCRECILNRWDKAREILREAHAEARRELGLPPEPPSEGLECGACVNRCRIPEGKVGYCGVVRNEGGKIIREHAGRAYLDPHPTNCVGAWVCPGATSRGYPKYTETRGPERGRYNLAVFYGGCTHHCFFCQNYEHWVERERLSAEDLKRRASDPDVTCVCFFGGDPTPHYDQVIEIGREWEELRVRVCLETNGSLRPKLMRRIAEICYESGGGFNMDVKAYDPRIYYALSFSDPEYTFKNLEIIGREFAREDPPILRPSTLVVPEYITPEEVEKIAEFLASIDERIPYRLLQHVPQYHSADLGYTPVCVMRECKRAAERHLENVDACIYRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL9</accession>
    <name>Q8TYL9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01494">
        <property type="protein sequence ID" value="AAM01494"/>
        <property type="gene ID" value="MK0277"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0277"/>
    <dbReference type="HOGENOM" id="CLU_1080107_0_0_2"/>
    <dbReference type="OMA" id="YVILYPH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016771">
        <property type="entry name" value="Fe-S_OxRdtase_rSAM_TM0948_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020275">
        <property type="entry name" value="RadSAM_TM0948_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="35"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="28384" checksum="45007A45274202A4" modified="2002-06-01" version="1">MLEWRPEPGYDPLETAERVRGWVCEGRRRKYYRFRETRFYGGCATADAVGCNLDCAYCYVNYPRRHPWDRRWKFHRPIDVVERLKNMGGDVVRVSGCEPTLCKEHILELIELCGRELPDRKFVLETNGTILGADRSFVRELGNHEHVHVRVCLKGYDPQSFARITNANPDGFDLQLRCLRYLFKEGISFHPAIPRLFRPEDIDKLAGVLSDIGVPPSSLEVEPIRVYDHVRRELRRRGLTVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM8</accession>
    <name>Q8TYM8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase, delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">flpD_1</name>
        <name evidence="3" type="ordered locus">MK0268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0268"/>
    <dbReference type="EnsemblBacteria" id="AAM01485">
        <property type="protein sequence ID" value="AAM01485"/>
        <property type="gene ID" value="MK0268"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0268"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.271"/>
    <dbReference type="HOGENOM" id="CLU_095272_2_0_2"/>
    <dbReference type="OMA" id="FPCTGRI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FlpD" evidence="2">
        <location>
            <begin position="8"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="141" mass="16208" checksum="E284E11679313DED" modified="2002-06-01" version="1">MGEWKPKIIAFCCNWCSYGGADTAGVGRMQYPPSTRIIRVMCSGRIEPSLVLKAFREGADGVFVGGCHLGDCHYQSGNYKWWRRAELLKKFLAEIGIEPERFRYEWISASEGEKFAEVMTEFHQTLLELGPLELRERLKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN4</accession>
    <name>Q8TYN4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0261"/>
    <dbReference type="EnsemblBacteria" id="AAM01478">
        <property type="protein sequence ID" value="AAM01478"/>
        <property type="gene ID" value="MK0261"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0261"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.264"/>
    <dbReference type="HOGENOM" id="CLU_1718221_0_0_2"/>
    <dbReference type="OMA" id="VNWIVNN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="7">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="153" mass="16167" checksum="872692F7D492DA92" modified="2002-06-01" version="1">MARVSPLVTGLVAGVSAAILQAVFKVSPPPAYGICIACHTRDLVNWIVNHAAGTTLGMAPVSKPFPVLTVVGIFIGALIAAFAHGEFRIRRTHHPILGFVLGFLVINFALFMGGCPIRTSLRTMYGDVVALLGLISIAVGVILGAEFYLKRKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR4</accession>
    <name>ARCH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0228</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYR4"/>
    <dbReference type="SMR" id="Q8TYR4"/>
    <dbReference type="STRING" id="190192.MK0228"/>
    <dbReference type="EnsemblBacteria" id="AAM01445">
        <property type="protein sequence ID" value="AAM01445"/>
        <property type="gene ID" value="MK0228"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0228"/>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="OMA" id="WLSELLY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="PTHR12682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="SSF69819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000068844" description="Protein archease">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <sequence length="140" mass="16358" checksum="35F7A44CBAE3C994" modified="2002-06-01" version="1">MPWEEIEHTADAAFRVWADTPEDLLVEAAKALFDLITDLDAVEPEEEVEIEAEGGDLVELLHDWLEEIHFRHEIDGMLFSDFEVKELKKEEGWKVRGVARGEPYDPDRHPFHTEVKAVTYHNMKVEREDGRWVAEYVVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW4</accession>
    <name>Q8TYW4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01394">
        <property type="protein sequence ID" value="AAM01394"/>
        <property type="gene ID" value="MK0177"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0177"/>
    <dbReference type="HOGENOM" id="CLU_2447692_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="89" mass="9476" checksum="8D0052715A24B4B5" modified="2002-06-01" version="1">MDALLKICILNALAVVAFAVLVWAAFRYALPSWFILAIIVPGVLGSACVDYVMISVGVYDGPWETAFASVVYAVAWWTGRPGPSGPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX1</accession>
    <name>Q8TYX1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0170"/>
    <dbReference type="EnsemblBacteria" id="AAM01387">
        <property type="protein sequence ID" value="AAM01387"/>
        <property type="gene ID" value="MK0170"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0170"/>
    <dbReference type="HOGENOM" id="CLU_793690_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="349" mass="38264" checksum="AB864881848FF745" modified="2002-06-01" version="1">MNPPELAAALPYSGCDNPREFLKRLEVRSDPISLALVEIHGRVARKVQEWKPFVCPDIRAAREFTREIVAKAKADFLAERAGGSEALDLCAGPGGDTLALAEHYDVKAVDREVPRIEALKINARLHAGHAVEVIELDVMEAELDADVVHADPGRSGAKDPKRTEPPATELRDMFSEVPHMIEVPPAVKPRPGTVVFSATGEVRSVCWTNLTEKVAAVIAETSAVLEGLPRKPTEALEPEEVRYVIEQDPAVRKANLSWKLAEELNVHPTVVAGEETVLASEDPPDLTVDHVIRVVEVGEEGDGPPTIRSLGVKLNPRRLAELRKTYKEYDIVYVTKAGVLAGKVIYERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX8</accession>
    <name>Q8TYX8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01380">
        <property type="protein sequence ID" value="AAM01380"/>
        <property type="gene ID" value="MK0163"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0163"/>
    <dbReference type="HOGENOM" id="CLU_1478918_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="44"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="182" mass="20179" checksum="2598985399401730" modified="2002-06-01" version="1">MSVWAFVRKALREPLRVGAPAPTRRETAEFMVRAAGVEEGDFVVDAGTGNGVVAIAAAEMGCEVLAVDVDPEMIDMARRNAEEYGVEDSIEFVVADARELPELVDNVDAVLSTVPVKTVPEPLEFLRSCATVLKTSGRFVQLTHWPGYFTKLLHHEVPLRVLEKYLKWVHIVPGFVFVCERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ10</accession>
    <name>Q8TZ10_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0131"/>
    <dbReference type="EnsemblBacteria" id="AAM01348">
        <property type="protein sequence ID" value="AAM01348"/>
        <property type="gene ID" value="MK0131"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0131"/>
    <dbReference type="HOGENOM" id="CLU_113977_0_0_2"/>
    <dbReference type="OMA" id="FIIEHFD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007162">
        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
        <property type="entry name" value="DUF366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
        <property type="entry name" value="UCP006503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="181" mass="20067" checksum="43647A976A74397B" modified="2002-06-01" version="1">MIVDREELEYDGSQLRRAFAHERYGIKGRAVVVFRGPMDVRTEYTADAEDVGSPIRGNDVLHLLVDDPTRADPLVAGLLQRLLVVVTKEVIEKELSTDLDRDGDDLLHDGRKLTVSVFKPAGPGSLAHLGINVTTEGVPVPASSLRDLGYRGDPLDLGRRVAVEFVREITDVELDLTKIRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ13</accession>
    <name>Q8TZ13_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0128"/>
    <dbReference type="EnsemblBacteria" id="AAM01345">
        <property type="protein sequence ID" value="AAM01345"/>
        <property type="gene ID" value="MK0128"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0128"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.127"/>
    <dbReference type="HOGENOM" id="CLU_021802_2_4_2"/>
    <dbReference type="OMA" id="VSGHKGM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="1">
        <location>
            <begin position="156"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="351" mass="38609" checksum="C792AA747D388449" modified="2002-06-01" version="1">MIRLDVRERVVKLLCDYISIPSVSGEEEELSERYASDLERAGLEVEIDRLGNVIGRRGEPEVCLTSHLDTVPPDEMEKPFEPRIVDGKLYGRGACDAKANLAVYATLAEIWDGPLEIIAVVREETDSAGIRHVLRRGEIQANHVINGEPTELRPVIGHKSRVEVRLCIEGEPKHAGSHNPENPILKFCKILHDLHEMLEDLEDALGVPTANPTSVHSRGVATNVTPQCLEAVLDVRLNTQLSPEDLERFFHEVEGVSAEIRAGAPPFVLSGDEPVVRALREALSARGLPDEPITWPASTDAGYIRNLGGKDVVVFGPGSIDYAHSPSEHVPIEELVDAVRVLYDVVEYLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ18</accession>
    <name>Q8TZ18_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01340">
        <property type="protein sequence ID" value="AAM01340"/>
        <property type="gene ID" value="MK0123"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0123"/>
    <dbReference type="HOGENOM" id="CLU_1405988_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="193" mass="20935" checksum="8BAA79B9D31C0612" modified="2002-06-01" version="1">MVEARALAEAVTKGVLTHVEVSVKPGSGLVLDVDVPRADRVKDIVRSLVTPITGMYQVRVRITNARAEPEAANKKCLATGTFTALGKALMDAFFPGRGTEAVNKALLRVPITSEEAYRGKRFDRVYDPRKKRWIPARPGMMCRTKDGMICAVTDDPDGIAPPAAAEKDAKLATVLCDTLFDMLLAEKPPTVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ22</accession>
    <name>Q8TZ22_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chorismate--pyruvate lyase family enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiC</name>
        <name evidence="1" type="ordered locus">MK0119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0119"/>
    <dbReference type="EnsemblBacteria" id="AAM01336">
        <property type="protein sequence ID" value="AAM01336"/>
        <property type="gene ID" value="MK0119"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0119"/>
    <dbReference type="HOGENOM" id="CLU_107938_2_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002800">
        <property type="entry name" value="Rv2949c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01947">
        <property type="entry name" value="DUF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="164" mass="19130" checksum="02861FCDE99CB69D" modified="2002-06-01" version="1">MEVPLLEVLLYTDGSVTRILERFFEEVTIDPLETRKLKVEGREARLLGVPDGDTVYVRRVVIKVDGRPAILATSLARPDNLPGRLRRLVLQSRKPLGKMIEELRLETRREILRVEEARPSPEDEEILDVSVPKIPWREYLVYHRRTPMLLIRERFNPEVLGREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ47</accession>
    <name>Q8TZ47_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0092"/>
    <dbReference type="EnsemblBacteria" id="AAM01309">
        <property type="protein sequence ID" value="AAM01309"/>
        <property type="gene ID" value="MK0092"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0092"/>
    <dbReference type="HOGENOM" id="CLU_063749_0_1_2"/>
    <dbReference type="OMA" id="IVVYVYE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="183" mass="19846" checksum="3FFDC573D9A47095" modified="2002-06-01" version="1">MITVLVLGDAHIPERAQEVPHTLKRKIEELAPVDVVISPGDYTTEDTIEWIASLGEKALMVVGNCDFGLPLPPRVTEDIGEVKVTVDHGSGVHPRGDPDQLAAIAEEEGADVIFTGHTHRPEFKEHRGVLIVNPGSLTGVPSGGGPSPGPSFMYGTIDGKEVWMKLYMLKGDRLETEEFETEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ62</accession>
    <name>Q8TZ62_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0077</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-monophospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13935"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0077"/>
    <dbReference type="EnsemblBacteria" id="AAM01294">
        <property type="protein sequence ID" value="AAM01294"/>
        <property type="gene ID" value="MK0077"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0077"/>
    <dbReference type="HOGENOM" id="CLU_051301_0_1_2"/>
    <dbReference type="OMA" id="RKIHREM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="PTHR12755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="36"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="385" mass="41992" checksum="F8D314B15034E321" modified="2002-06-01" version="1">MELVLGTGAGYSVEEDGVEIPSDWKELAEELADHGGTPVCMVIGPQDSGKTTLVTFIANELVERGLKVGIVDADVGQSDVGPPAVVSLGIVEDTVHDLSEVEMRHGYFVGSITPSGHLLQTTVGTRRMVDLALAEGTDVVLIDTSGMVHGGPARALKLHKVDAIRPSHVAFLDRDGQVSHIKRMVKSLKYIKVHDLAVPDAVKDVERKDRIRRRERVLREFFEEREILELDLEEVSVQRAFIGTGEPVDLEEESELSALIKAVSGVEPEILHAERAPDAVVLVVKDQAGRIVGRGGRHARELRRLLNVREFVVVNEEELQGVLVGLCDGAGDLLGIGVIREIDFASGELKVEGRLLRDRTIRVVQLGSLKVDPETGSHEPMNVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ72</accession>
    <name>Q8TZ72_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01284">
        <property type="protein sequence ID" value="AAM01284"/>
        <property type="gene ID" value="MK0067"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0067"/>
    <dbReference type="HOGENOM" id="CLU_680800_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017199">
        <property type="entry name" value="UCP037409_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09930">
        <property type="entry name" value="DUF2162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="404" mass="42613" checksum="CA956AFA98C8E3AB" modified="2002-06-01" version="1">MFHGVIAAFTTYLGASAALMLRYHSVGVSEALTYGAVYGMAAAAIGVGTGVMLTRVLGARKKRREVIRTVLEGTALSGVALLVGLLDVTAGAIAASSVALVYPSYRISRDLGVSPFKIVTLLAVTSSVIVAALTSWMIVPAFADNPIYALAIAFFVFIVGLKTGLGIGFAGFQLRRGAMVIAALSGILIPAIIGLSHLVSKFMKTALAMQRYALLGHALLGLFLIGLGIFIARKWAVEGSAKDLSKLGAFVVAVPCPICLTGVALSIAMYAEIGALNVTQAALVLWTCFVSTAYATLIVADAVIEVLKVPPAVALSGLENLVGLLFVITGIIAWAYPLWKRAPKLKLALPSPSKIGPWALLFLALMAFGYLWAVRKASRGNYPPDNPIDDIIRAVIEWLASRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ76</accession>
    <name>Q8TZ76_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0063</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01280">
        <property type="protein sequence ID" value="AAM01280"/>
        <property type="gene ID" value="MK0063"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0063"/>
    <dbReference type="HOGENOM" id="CLU_2893258_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="62" mass="7443" checksum="D459BB9186F3C8AA" modified="2002-06-01" version="1">MMLPEHIICASCGKRVGRKRTPPECPYCGEKKFVRMDPEERLGKGPEWWKEAERERRVRERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ94</accession>
    <name>Q8TZ94_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01259">
        <property type="protein sequence ID" value="AAM01259"/>
        <property type="gene ID" value="MK0042"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0042"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.42"/>
    <dbReference type="HOGENOM" id="CLU_876064_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="317" mass="33959" checksum="37DED60CB9C1B838" modified="2002-06-01" version="1">MRALALLALVLAAVGGAAAEEAPLYGVLTHPWTFQLSANQAKQIPKEYVISVEAPAGAQLPLGKAYYGVFVADGNHAALIVDLTPAVTGETDDAVVVDPNAMSGKLPPAVKFRQWKPAHLAKGALLALSLETKPLPVGLSLKMVAPEELTIEVGKFQVKLNRELLEGLARVLATHEYGHPHGKKKTPVGYLQALVFKYFVKASVTGAEPEELQKLEVSEEEKEGEEEKEVDEETGYALRAFLEVNEQQGLTDLKAEFSVDNLENLIGEAVTVLQDVLSSPEAQQAAATAESALKKFVQVPVAAVCLLGIALVAVRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ98</accession>
    <name>PELO_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein pelota homolog</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pelA</name>
        <name type="ordered locus">MK0038</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ98"/>
    <dbReference type="SMR" id="Q8TZ98"/>
    <dbReference type="STRING" id="190192.MK0038"/>
    <dbReference type="PRIDE" id="Q8TZ98"/>
    <dbReference type="EnsemblBacteria" id="AAM01255">
        <property type="protein sequence ID" value="AAM01255"/>
        <property type="gene ID" value="MK0038"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0038"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.38"/>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="Transl-rel_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PTHR10853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="SSF159065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000361802" description="Protein pelota homolog">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <sequence length="353" mass="40007" checksum="122F2A715C4BC6EC" modified="2002-06-01" version="1">MKVVEKDLDKGYIEVLPETLDDLWHLYHVVRKGDLVFALERRRVKDERAETIRRDKGERKPVYLGVRVEDVEFDKYANRLRIKGVIEHGPESGSHHTVNVTTGKRIKIVKDEWERKDLERIEEAEMSRPPVMLVAVDTGEGTIGIVRDYGLDVVARVRHNVPGKRGGDRRAEMRKFFHRLADEIERIAEEEGVEHIVVGGPGFVKSDFAEFLREERDIPAHVEDTGSAGEAGLIEMIRRGAVERAVEESRVAEEVKHLEEVFKRIGKGDDKVAYGVRECLKAAEFGAIDVLLVADEKFREAMVEGEEDVLNAVKYAERTGAEVLIVSTEHEWGERLRELGGIAALLRFSIPTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8X261</accession>
    <name>KPTA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable RNA 2'-phosphotransferase</fullName>
            <ecNumber evidence="1">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kptA</name>
        <name type="ordered locus">MK1434</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2000-10" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Belova G.I."/>
                <person name="Malykh A.G."/>
                <person name="Kozyavkin S.A."/>
                <person name="Slesarev A.I."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the 2'-phosphate from RNA via an intermediate in which the phosphate is ADP-ribosylated by NAD followed by a presumed transesterification to release the RNA and generate ADP-ribose 1''-2''-cyclic phosphate (APPR&gt;P). May function as an ADP-ribosylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AF311944">
        <property type="protein sequence ID" value="AAL61957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8X261"/>
    <dbReference type="SMR" id="Q8X261"/>
    <dbReference type="STRING" id="190192.MK1434"/>
    <dbReference type="EnsemblBacteria" id="AAM02647">
        <property type="protein sequence ID" value="AAM02647"/>
        <property type="gene ID" value="MK1434"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1434"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1590"/>
    <dbReference type="HOGENOM" id="CLU_052998_4_1_2"/>
    <dbReference type="OMA" id="TEEPVHC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016772">
        <property type="term" value="F:transferase activity, transferring phosphorus-containing groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00299">
        <property type="entry name" value="KptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022928">
        <property type="entry name" value="RNA_2'-PTrans_KptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PTHR12684"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000157488" description="Probable RNA 2'-phosphotransferase">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00299"/>
        </source>
    </evidence>
    <sequence length="234" mass="27356" checksum="FEB9DDC384EEF521" modified="2002-03-01" version="1">MKPIRVCPECGKYTEKHTCERCGRRTEEFLDGRRRLALSKLLSGILRHFPEEVKVKLDDEGFTDCDVHELAERIKKYWKNREYYRWLTGEHIIAVVETCPKGRFEIDEHGRIRARYGHSRRLSVRPTLPEAENVKELYHGTARENLESILQHGIKPMGRRAVHLTDDEREALITALRHTRNPVILVVDAERLRRHGLVPRKAGKNVYVVEGTVPPDCITRVIRNPRRSVESEKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>O32866</accession>
    <name>MTRB_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit B</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrB</name>
        <name type="ordered locus">MK0659</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="250" first="783" last="788">
            <title>Identification of the active site histidine in the corrinoid protein MtrA of the energy-conserving methyltransferase complex from Methanobacterium thermoautotrophicum.</title>
            <authorList>
                <person name="Harms U."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9461302"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00783.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="Y14428">
        <property type="protein sequence ID" value="CAA74770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O32866"/>
    <dbReference type="SMR" id="O32866"/>
    <dbReference type="STRING" id="190192.MK0659"/>
    <dbReference type="EnsemblBacteria" id="AAM01874">
        <property type="protein sequence ID" value="AAM01874"/>
        <property type="gene ID" value="MK0659"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0659"/>
    <dbReference type="HOGENOM" id="CLU_171544_0_0_2"/>
    <dbReference type="OMA" id="TNMVYGF"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01094">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008690">
        <property type="entry name" value="MtrB_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05440">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005518">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04166">
        <property type="entry name" value="methano_MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147515" description="Tetrahydromethanopterin S-methyltransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="112" mass="12227" checksum="FE83EF77EB8583BA" modified="1998-01-01" version="1">MAIVLIDPESQIAMDAVTGAVAEWSEDVVTLDVMPLYEKVEELEQYVNDMMRAMDPSTTTWGTLPGREGVHETAGFLTNFAHGFVIGTMIVALVAFTLAAVYKLHALRLLGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>P96801</accession>
    <name>HDRA2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2</fullName>
            <ecNumber evidence="5">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrA2</name>
        <name type="synonym">hdrA_2</name>
        <name type="ordered locus">MK0265</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="244" first="226" last="234">
            <title>Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri is not a flavoenzyme.</title>
            <authorList>
                <person name="Kuenkel A."/>
                <person name="Vaupel M."/>
                <person name="Heim S."/>
                <person name="Thauer R.K."/>
                <person name="Hedderich R."/>
            </authorList>
            <dbReference type="PubMed" id="9063468"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.00226.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 4 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="5"/>
    <dbReference type="EMBL" id="Y09871">
        <property type="protein sequence ID" value="CAA70999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P96801"/>
    <dbReference type="SMR" id="P96801"/>
    <dbReference type="STRING" id="190192.MK0265"/>
    <dbReference type="EnsemblBacteria" id="AAM01482">
        <property type="protein sequence ID" value="AAM01482"/>
        <property type="gene ID" value="MK0265"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0265"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.268"/>
    <dbReference type="HOGENOM" id="CLU_020302_0_0_2"/>
    <dbReference type="OMA" id="TAKHAML"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000150059" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit A 2">
        <location>
            <begin position="1"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="4">
        <location>
            <begin position="238"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="4">
        <location>
            <begin position="286"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="4">
        <location>
            <begin position="577"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 4" evidence="4">
        <location>
            <begin position="610"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="589"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="592"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="596"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="629"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LWL2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8TM02"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="656" mass="71925" checksum="C7CF218545ACEA7C" modified="1997-05-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-04-18" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS2</accession>
    <name>RTCB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="1">6.5.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mka hyp2 intein</fullName>
                <ecNumber>3.1.-.-</ecNumber>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">rtcB</name>
        <name type="ordered locus">MK1682</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="1290" last="1295">
            <title>Archaeal 3'-phosphate RNA splicing ligase characterization identifies the missing component in tRNA maturation.</title>
            <authorList>
                <person name="Englert M."/>
                <person name="Sheppard K."/>
                <person name="Aslanian A."/>
                <person name="Yates J.R. III"/>
                <person name="Soll D."/>
            </authorList>
            <dbReference type="PubMed" id="21209330"/>
            <dbReference type="DOI" id="10.1073/pnas.1018307108"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Ligation proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RtcB reacts with GTP to form a covalent RtcB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="1"/>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUS2"/>
    <dbReference type="SMR" id="Q8TUS2"/>
    <dbReference type="STRING" id="190192.MK1682"/>
    <dbReference type="EnsemblBacteria" id="AAM02895">
        <property type="protein sequence ID" value="AAM02895"/>
        <property type="gene ID" value="MK1682"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1682"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1847"/>
    <dbReference type="HOGENOM" id="CLU_012374_0_0_2"/>
    <dbReference type="OMA" id="VHNCGVR"/>
    <dbReference type="BRENDA" id="6.5.1.8">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008452">
        <property type="term" value="F:RNA ligase activity"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000304"/>
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</entry>
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                <person name="Mezhevaya K.V."/>
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                <person name="Shakhova V.V."/>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">This protein undergoes a protein self splicing that involves a post-translational excision of the VDE intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUT0"/>
    <dbReference type="STRING" id="190192.MK1673"/>
    <dbReference type="PRIDE" id="Q8TUT0"/>
    <dbReference type="EnsemblBacteria" id="AAM02886">
        <property type="protein sequence ID" value="AAM02886"/>
        <property type="gene ID" value="MK1673"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1673"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1837"/>
    <dbReference type="HOGENOM" id="CLU_301612_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="PTHR43389"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0404">Intron homing</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000002476" description="V-type ATP synthase beta chain, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002477" description="Mka AtpB intein" evidence="1">
        <location>
            <begin position="261"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002478" description="V-type ATP synthase beta chain, 2nd part" evidence="1">
        <location>
            <begin position="778"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="491"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="990" mass="110354" checksum="7AC6BE8A32B31585" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-28" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY3</accession>
    <name>RL39_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L39e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl39e</name>
        <name type="synonym">rpl39</name>
        <name type="ordered locus">MK1618</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY3"/>
    <dbReference type="STRING" id="190192.MK1618"/>
    <dbReference type="EnsemblBacteria" id="AAM02831">
        <property type="protein sequence ID" value="AAM02831"/>
        <property type="gene ID" value="MK1618"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1618"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1781"/>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="OMA" id="KMRYWRR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_L39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_L39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_L39e_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="SSF48662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127051" description="50S ribosomal protein L39e">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <sequence length="51" mass="6011" checksum="D4350C7BF56CBEB2" modified="2002-06-01" version="1">MARVKPLGKKLRMAKAIKQNRRVPPWVVAKTGGRVIDNPKRRHWRRSKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV00</accession>
    <name>Q8TV00_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1601</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02814">
        <property type="protein sequence ID" value="AAM02814"/>
        <property type="gene ID" value="MK1601"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1601"/>
    <dbReference type="HOGENOM" id="CLU_1431654_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="154"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="189" mass="20010" checksum="3C0AAEB5D28020FA" modified="2002-06-01" version="1">MGELADAVREHLVSATLNAIGLIVVILVIQLVVPPLASLGTMIPGIGVPVNMVLTAVGVVLALYFAYGVLKHVKPMITPAADLVSMVLLGRREEDLRTATYNLVLAAIVLVVAVLLSPLMVSVPGAGAVLSLLVLLVGIGFGGLLLIKAATGFYEVFRDKLEELAENLAERVEELERKASETEETEESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV11</accession>
    <name>Q8TV11_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UDP-N-acetylmuramyl pentapeptide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murF</name>
        <name evidence="1" type="ordered locus">MK1590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1590"/>
    <dbReference type="EnsemblBacteria" id="AAM02803">
        <property type="protein sequence ID" value="AAM02803"/>
        <property type="gene ID" value="MK1590"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1590"/>
    <dbReference type="HOGENOM" id="CLU_047362_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="366" mass="39565" checksum="C1F9B61A5E80A262" modified="2002-06-01" version="1">MSDELHGIGHEVVLVDIYGTCDEARPYPILRRAFGRFDFIAVPVHCGVDFGTVEGPRVTHHALAGALAARYKQDFLFEVTGVRGKTTTATYLAWILEEAGHRPALSTTDESPVGRPSVTPARVVEVVRETSGPYVCEVSLGVTSAADYAVFTGAPYDYPIAGGSSSALRAKKKTLLESGAEVMIEYREAVKLSLVGPKVHRVHTSDGTVKCDDICVEFEAFDIPFHDRCFGLAAATALTSGLADREDVEAARARGPVPSRLELRRNELVDAHSAVNEQTVRYALSVASDLWGRYGAVIGGTLGGYCEGVDPEAVAEVLQERIERGELVALKLKGELGREVERHLENVDLPEPGPDTPIVRIVRKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV27</accession>
    <name>Q8TV27_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiA</name>
        <name evidence="3" type="synonym">cfbB</name>
        <name evidence="6" type="synonym">cobB_1</name>
        <name evidence="6" type="ordered locus">MK1573</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP + 2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP + cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="3"/>
    <dbReference type="EC" id="6.3.5.12" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1573"/>
    <dbReference type="EnsemblBacteria" id="AAM02786">
        <property type="protein sequence ID" value="AAM02786"/>
        <property type="gene ID" value="MK1573"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1573"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1734"/>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="OMA" id="MYLTNSI"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="249"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="3">
        <location>
            <position position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="445" mass="48987" checksum="92231057620F0020" modified="2002-06-01" version="1">MLAVAIAGVRSGCGKTSLSIGLVRALREEGTRVRPFKVGPDYIDPTYLEVAAGLEAFNLDVWMMGESGVVDAFGRYTRRCDAAVIEGVMGMFDGHGEGTVEGSTAHVAALLGVPVVLVVDCRSYATTVAAEVRGFRDMAHEIGTRLEGVILNRVGSERHERLLITALRRYCPDVRVFGVIPRLEDAVIPDRHLGLIPADERNEFAERVAEYWGEVVAEHVDLDALRELEVEGPSVEPSPPPEPERVETKVAVISGRVFTFYYRENLRFLAESAEVVPVDPEKDRLPDVDAVYIPGGYPEVYADNLDDRLMRDLLEFHEEGGRILGECGGLMYLCSEVVDADGDRHDMVGVFDAVARMRERLAALGYVEGRVTDAHPFAPSGSEIRGHEFHYSVVDVREDLEYAYRLSRGTGVREGLDGLVKGNTVASYTHLHIRSDGGVFRGLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV51</accession>
    <name>Q8TV51_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-binding domain of the Kef-type K+ transport system fused to a uncharacterized conserved domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">kch_3</name>
        <name evidence="3" type="ordered locus">MK1549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1549"/>
    <dbReference type="EnsemblBacteria" id="AAM02762">
        <property type="protein sequence ID" value="AAM02762"/>
        <property type="gene ID" value="MK1549"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1549"/>
    <dbReference type="HOGENOM" id="CLU_764225_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035922">
        <property type="entry name" value="3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004173">
        <property type="entry name" value="3H_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02829">
        <property type="entry name" value="3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75500">
        <property type="entry name" value="SSF75500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="125"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="380" mass="42522" checksum="BFE376C7155EDDF9" modified="2002-06-01" version="1">MKAKVRTFRYLWHLLRDVISVPTVKYAVAYVAALLCLGTLGYWTLEGRSPVDAFYTTVLILTGVGCANPPTTPAGEIFTVGLLAVGLGALIHIISRVFAALLRGDVLLRIKESDAMARIERMRDHVVICGYGKKGREIARNLGEHGFEVVVVDKDPEKCDRAFRDGHLAVQGDVTSEETLLKAGVERAQAVALVTDSDETNVFACVLVRDLNPDAWIVAAARSKTGARTLLRAGADEVVRVYEAAGIVIANRLMDPLSFLVTVRHPLEDTFREFREIIRHGGIVVDVRYHIPSLPEPLVKDLWVEDESDVKRRLEMHEDSETREALERLHRMSDDVHSHRIIVRREEDKEKIVEALRRLVPDGVDMTHKEVLKEVFGVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV77</accession>
    <name>Q8TV77_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cbiB</name>
        <name evidence="12" type="synonym">cobD</name>
        <name evidence="13" type="ordered locus">MK1516</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1516"/>
    <dbReference type="EnsemblBacteria" id="AAM02729">
        <property type="protein sequence ID" value="AAM02729"/>
        <property type="gene ID" value="MK1516"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1516"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1674"/>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="OMA" id="NSGYTMA"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAM02729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="298" mass="32685" checksum="93E4A5023205F979" modified="2002-06-01" version="1">MPLDPLLAAYLAVALDLVLGDPPNRFHPVAWCGRVMELVEKRVRRGRVLDVVWGGLTLAVGCGFLLGVCYPLHYIPMVGDALVLWVCISVRGLVEHLLPVERELREGNLDGARQAVRWLVSRDVSRLNGAEVASAAVESCFENLLDSVVGPLWWYYLLGWPGAVMYRATNVADAMFGYRGEYEMFGKIPARLDDLLNVLSLPVCAAALTVTLPWWILRARLRSLLRAARDVPSPSSWLPMYVGACALGVRLEKPNVYVLKGGDRLPTPDDVRRAAQIAVIFGLVAPGITYVAAGLLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV86</accession>
    <name>Q8TV86_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diadenylate cyclase</fullName>
            <shortName evidence="6">DAC</shortName>
            <ecNumber evidence="6">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="6">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dacZ</name>
        <name evidence="8" type="ordered locus">MK1507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02720">
        <property type="protein sequence ID" value="AAM02720"/>
        <property type="gene ID" value="MK1507"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1507"/>
    <dbReference type="HOGENOM" id="CLU_1159065_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="79"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="239" mass="25665" checksum="46E8C6F52D7CF743" modified="2002-06-01" version="1">MNFVETFRRALRCLQPSAVVVDFDPPAESLRSTPVIKLPSGILHSVGPVEACASLGLKEGEPVLIASYSRCSLEFRPIRPEEARLAALDHYRVVSRILETMVELAVEGVEGDRVGTLCVISEGADRMYRTTVPVRVEDVNVMTGSGKRVLKALAKLDGAIVVDPEGNIEAAGAIFDVDSGSVEPGFGARHTTALHVSKELNCPVIVLSESGRIRLYHGGKEVLKISVSDLVAIRGFCDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA6</accession>
    <name>Y1486_METKA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0107 protein MK1486</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1486</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0107 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVA6"/>
    <dbReference type="SMR" id="Q8TVA6"/>
    <dbReference type="STRING" id="190192.MK1486"/>
    <dbReference type="EnsemblBacteria" id="AAM02699">
        <property type="protein sequence ID" value="AAM02699"/>
        <property type="gene ID" value="MK1486"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1486"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1644"/>
    <dbReference type="HOGENOM" id="CLU_141583_2_0_2"/>
    <dbReference type="OMA" id="CEPIVAT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="AcnX_sub2_archaea_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="AcnX_sub2_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="AcnX_swivel_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="DUF126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152566" description="UPF0107 protein MK1486">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="135" mass="14226" checksum="67015988A6857C95" modified="2002-06-01" version="1">MSVSPVRCEPVVDVEEPVEAEVLVSSQRLSFLGGVDPKTGEVVDPSHELCGEKLTGRVLVLPGGRGSTVGSYVLMEMADRGTAPAGIVVREAEPILVVGCVLGDIPLFHRPERDLVEELSTGDVVKLLPGGKVEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB5</accession>
    <name>RS9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps9</name>
        <name type="ordered locus">MK1477</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB5"/>
    <dbReference type="SMR" id="Q8TVB5"/>
    <dbReference type="STRING" id="190192.MK1477"/>
    <dbReference type="EnsemblBacteria" id="AAM02690">
        <property type="protein sequence ID" value="AAM02690"/>
        <property type="gene ID" value="MK1477"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1477"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1633"/>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="OMA" id="FMGQAEA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_S9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_S9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000111466" description="30S ribosomal protein S9">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="134" mass="15114" checksum="2D976679566FDADC" modified="2002-06-01" version="1">MGRVVQTTGKRKTAIARAVIREGEGRVRVNKRPVNIIEPEMARMKIMEPLIIAGEDIVSQVDIDVKVQGGGWMSQAEAARIAIARGLVEWTGDPDLRDAYMAYDRHMLKGDPRRKEPKKFGGRGARARRQKSYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH1</accession>
    <name>NADE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="1">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadE</name>
        <name type="ordered locus">MK1418</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVH1"/>
    <dbReference type="SMR" id="Q8TVH1"/>
    <dbReference type="STRING" id="190192.MK1418"/>
    <dbReference type="EnsemblBacteria" id="AAM02631">
        <property type="protein sequence ID" value="AAM02631"/>
        <property type="gene ID" value="MK1418"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1418"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1574"/>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="OMA" id="MVVQYAL"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152225" description="NH(3)-dependent NAD(+) synthetase">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="284" mass="31191" checksum="9E1DFC14237F2E85" modified="2002-06-01" version="1">MAKYEPVIPELEVNPEGEVSKIAEFLRGKFEEAGREIAVVGLSGGVDSSTTLGLAVEALGRENVVALILPERDTPEEDVEDAVEAAERFGVEYHVHDITEVLRAFGTGSYVPCHPFSRKSDANLKPRVRMCVLYYFANELDGLVLGTGNRTEWLTGYFTLHGDGACDVAPIRHLYKTQVYVIAEHLGVPERIVEEKEPSARLWPGQTDEGELGIDYPTLDALLYALVDEGLGPRKAVDWLGERGVEATEEDAEKVLDLVRSSSFKRRPAPGLDLPEPEDPAMSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH6</accession>
    <name>Q8TVH6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02626">
        <property type="protein sequence ID" value="AAM02626"/>
        <property type="gene ID" value="MK1413"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1413"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1569"/>
    <dbReference type="HOGENOM" id="CLU_1574966_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="169" mass="17748" checksum="47A9B36C133E5C22" modified="2002-06-01" version="1">MDPVFHVAASYALARAAGYPVRTSLMYAFGINVAIDLDHLLRYRWILHSPVALLAGVATAYAAGLRTGLVLALYTFHLLCDALAGMKGAGTGVPAMFPFSLKSYGLEAYVLLGPKGISWSLAPIARSSEAGPAGFLVSGVGLATTLGVVIFELLGVFKKRRELALAPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI0</accession>
    <name>Q8TVI0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="2">ADH synthase</shortName>
            <shortName evidence="2">ADHS</shortName>
            <shortName evidence="2">ADTH synthase</shortName>
            <ecNumber evidence="2">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fbaB</name>
        <name evidence="2" type="synonym">aroA'</name>
        <name evidence="3" type="ordered locus">MK1409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1409"/>
    <dbReference type="EnsemblBacteria" id="AAM02622">
        <property type="protein sequence ID" value="AAM02622"/>
        <property type="gene ID" value="MK1409"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1409"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1564"/>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="OMA" id="MHVNIGA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01949">
        <property type="entry name" value="AroFGH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="27"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="DKFP binding" evidence="2">
        <location>
            <begin position="231"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="29118" checksum="B74F66C5CE2FCCD6" modified="2002-06-01" version="1">MVHIGKQIRMERIMNRETGRTLIVPMDHGVTLGPITGLEDLEETVDAVARGGANAVLLHKGMVRAGHRGYGRDVGLIIHLSASTELGPDPNNKVLVSRVEEAIRLGADAVSVHVNVGAEDEPQMLKKLGEIAARCSDWGMPLVAMMYPRGPKVEDEFDVEYVKHAARVGAELGADIVKTNYTGDPDSFREVVKGCPVPVVIAGGPKAETPKEVLEMVKGAIEAGAAGAAIGRNIFAHKSPRMREAMTRAIARIIHEDAEVEEAMKELERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ2</accession>
    <name>Q8TVJ2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="3">DHBP synthase</shortName>
            <ecNumber evidence="3">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ribB</name>
        <name evidence="4" type="ordered locus">MK1397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1397"/>
    <dbReference type="EnsemblBacteria" id="AAM02610">
        <property type="protein sequence ID" value="AAM02610"/>
        <property type="gene ID" value="MK1397"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1397"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1552"/>
    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="OMA" id="DAGGLIC"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="25641" checksum="098556AF0773528D" modified="2002-06-01" version="1">MRTVRRALRRGEPVFIFDSEDREGETDMVFWAPEVTPDHVAELRRTAGGLICVVIHPEHAREIRLPFLVDVYERADHPLLRATWPDDIPYDERSTFSITVNHRDTFTGVTDEDRALTVRKLAEFFTRNHEDPVREFGEEFRSPGHVHLLRPFDGLLEERRGHTELTATLLELVGLEPKVAVICEMLEPGGGALPREEAERVARERGAPFLTGEDILKAWKGGER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ8</accession>
    <name>TRPC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="1">IGPS</shortName>
            <ecNumber evidence="1">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpC</name>
        <name type="ordered locus">MK1391</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVJ8"/>
    <dbReference type="SMR" id="Q8TVJ8"/>
    <dbReference type="STRING" id="190192.MK1391"/>
    <dbReference type="EnsemblBacteria" id="AAM02604">
        <property type="protein sequence ID" value="AAM02604"/>
        <property type="gene ID" value="MK1391"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1391"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1546"/>
    <dbReference type="HOGENOM" id="CLU_034247_0_1_2"/>
    <dbReference type="OMA" id="RGPHDLI"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154293" description="Indole-3-glycerol phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <sequence length="254" mass="27773" checksum="E27B3A794314A394" modified="2002-06-01" version="1">MDEILERVRERVEEIRDRTFTPRAGGRSLRKALSGPGVSVIAEVKPTSPSQGRLRDVDAEDVAERARAYERGGAAGISVLTEPEFFDGRPEYVEVVREAVDVPVLRKDFIIDPVQVEESAHYGADAVLIIAAAVGREAPELIDLAHEHGMEVLLEIDRWEHLELLSECDPDVVGVNNRDLRTLEVDLNRTLELGPEVKDLTNAPLVAESGVSGPEDVVLLGEVADAVLVGTYLMRAPDPSEAVRKLVEAGRSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVK2</accession>
    <name>Q8TVK2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK1387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1387"/>
    <dbReference type="EnsemblBacteria" id="AAM02600">
        <property type="protein sequence ID" value="AAM02600"/>
        <property type="gene ID" value="MK1387"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1387"/>
    <dbReference type="HOGENOM" id="CLU_848910_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="35123" checksum="6A5EAB22FC25EEFB" modified="2002-06-01" version="1">MERSLLIKTILGAVAGAIALYLTLFHLADVDEVLRALQRADVAWILAAGACEVLWFAACVEGWKKTFEPLGGRPSRRQLFLMYCVKLMVNNLISLARVLGDAIRVYYGIRLGWSAATVIPTIVADIVLGNAGYLAVILLGCAIVWCCTEVSPYLIAANGVGAVAVAGLWALFASERTCHEAYESVRDLVEALVRRVGYSVGTVDELVDSTVQLFRSKEARLALLQYTVGWAARVLRLYCVTWAVWPTASPLIPLVMSVAIRGSAVVSVSPGGLGIVEGLTTGVLTVLEADPSRVMAALLLDRLYSFVIPVALGAVSVPVLERYVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM6</accession>
    <name>Q8TVM6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">A/G-specific DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nth_2</name>
        <name evidence="6" type="ordered locus">MK1362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1362"/>
    <dbReference type="EnsemblBacteria" id="AAM02575">
        <property type="protein sequence ID" value="AAM02575"/>
        <property type="gene ID" value="MK1362"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1362"/>
    <dbReference type="HOGENOM" id="CLU_012862_2_1_2"/>
    <dbReference type="OMA" id="RNMIRVI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="5">
        <location>
            <begin position="43"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="206" mass="23078" checksum="D19C3BA13B069DA5" modified="2002-06-01" version="1">MATLLEAPRIVERWLRGMEERGEYEPAWGDREDVYSVALAGVLHQRTRRELAEPVLRELLRRYPEPSDLLKAPEDELKESLARIGLVERRLKAVLGLARLLSEDPEPSGEDLLSVPGVGPYTADLVRAVVYRERVLPVDANVRRVVRRSTGRPVGDVGAEWVRAARDPRDLALGTVELGRRCCRPEPECEECPIAGVCAEQGRVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM7</accession>
    <name>Q8TVM7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Calcineurin superfamily phosphatase or nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02574">
        <property type="protein sequence ID" value="AAM02574"/>
        <property type="gene ID" value="MK1361"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1361"/>
    <dbReference type="HOGENOM" id="CLU_437991_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039331">
        <property type="entry name" value="PPA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22953">
        <property type="entry name" value="PTHR22953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="232"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="585" mass="66181" checksum="0BFDB2386C4783E4" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR6</accession>
    <name>Q8TVR6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02535">
        <property type="protein sequence ID" value="AAM02535"/>
        <property type="gene ID" value="MK1322"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1322"/>
    <dbReference type="HOGENOM" id="CLU_1544212_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="18479" checksum="13C7CD2401FC159E" modified="2002-06-01" version="1">MKGVTVRFEGLTALRDVDLSVEEECGSSSRASTWPRVAYSRVAFGMRYFPSAEGWGFVLLGTVASASVGALVALLCREEKSASAVLNAVASPAMFLGAVVVPENLVPRVAFEIFHRYPPVTFIHALRATDLYGKPVAVADVVVGTVLTATVFAASALVFRRAARELRCRNSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR7</accession>
    <name>Q8TVR7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02534">
        <property type="protein sequence ID" value="AAM02534"/>
        <property type="gene ID" value="MK1321"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1321"/>
    <dbReference type="HOGENOM" id="CLU_3039056_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="54" mass="6397" checksum="AE410E1D64143E5B" modified="2002-06-01" version="1">MFVVLFHEHITANPKYWWIILEFVDYARSHPGVAFATIDEFAELARHGQRFTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV4</accession>
    <name>Q8TVV4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1284"/>
    <dbReference type="EnsemblBacteria" id="AAM02497">
        <property type="protein sequence ID" value="AAM02497"/>
        <property type="gene ID" value="MK1284"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1284"/>
    <dbReference type="HOGENOM" id="CLU_123170_1_1_2"/>
    <dbReference type="OMA" id="DAYINAR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="141" mass="16089" checksum="1B45758B7CCE08B2" modified="2002-06-01" version="1">MAPSGSYEEARSLWERAGEALELARFSLEEGMYRWTAFHAEQAVRLGIKAALAFLTGEYPRVRGLSELLGVLVDVTGDRRVRELAGELREELKMLSDAYTAARYRVEFPYDTREDAERFIETAERVLSTLGELLREHGFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW4</accession>
    <name>Q8TVW4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted hydrolase of the metallo-beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02487">
        <property type="protein sequence ID" value="AAM02487"/>
        <property type="gene ID" value="MK1274"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1274"/>
    <dbReference type="HOGENOM" id="CLU_830582_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="7"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="334" mass="37094" checksum="68CC150A75250079" modified="2002-06-01" version="1">MLLRPLDPWCGVVVDYGDTRVFVDVTDRKGPYVKRADAVIVTHPHRDHYGGVPRTEFGELRADEVTAALLGMEDEAEPLRSSFEAGLLDVRTYPVNHMCPKAHAVLLSGDARVLITGDWCALGEHPPLWELVDDEVDAIVTECTRAASVHPDDPEAAEYRIQRLLELHREDRTVLLLTHVNPLIDVASEVEILHVLEGDPVDEAVKAGAHEGADLEVVSRPEYPLVTSSVGTVEGLSKRPHVLITERWFLYRDREGRLRRLLDAVPYPYIVSGTGHASAAELVRLVEELEPEYLVLRHGSPDGVRALRKRLAGAGLDVRVEAWIGRRGGKLELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-10-07" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX7</accession>
    <name>Q8TVX7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe2+/Zn2+ uptake regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fur_2</name>
        <name evidence="3" type="ordered locus">MK1261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BlaI transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02474">
        <property type="protein sequence ID" value="AAM02474"/>
        <property type="gene ID" value="MK1261"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1261"/>
    <dbReference type="HOGENOM" id="CLU_1933264_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005650">
        <property type="entry name" value="BlaI_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03965">
        <property type="entry name" value="Penicillinase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="130" mass="14867" checksum="5DC704B1DF1512F7" modified="2002-06-01" version="1">MLEERELEELAKALKRGELDKTGAVLGLLYLAYVETGGHGGMTADDVVRVLQESGYDITKERFYSLASQLMKKGYIESHKINGRRVIYTINSDSEYEVEQKLRERLEKSAPTAERVSAEEMRRILDRHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY7</accession>
    <name>Q8TVY7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02464">
        <property type="protein sequence ID" value="AAM02464"/>
        <property type="gene ID" value="MK1251"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1251"/>
    <dbReference type="HOGENOM" id="CLU_800798_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="38765" checksum="C69C72E66F7C792F" modified="2002-06-01" version="1">MKELMSSPRGDPEFPLRAEPLAPVYVPRTQFLKDVDGDGRVDHLHPSEAAALEYLIALGHDLDPFDVERDSRPMELGSLLSPAVEDLRDVGLPVVPSTVRPPDASWAAAILTYLLEEVPIAYGPKRTVLSFSAVYRTPVEHRPMRTAPDVEVDHECVVVQTDSRYVYVHCTRYGVAAVRRDVFDSACGVLATTVDHSPPPEDAGPSEVADHVLTETERPVLAAVDSEHLNPEHATGTVPFVVAVEEGWMEDWKEGVEHDLPADGIGLASYLATLTLRDRLPVYHRDKDGSFKEWLREHEREIIGRARPEEIDPVVEIAQKLEGCLEDLKTPEDDLHRLFDFLRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW00</accession>
    <name>Q8TW00_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02451">
        <property type="protein sequence ID" value="AAM02451"/>
        <property type="gene ID" value="MK1238"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1238"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1342"/>
    <dbReference type="HOGENOM" id="CLU_1192633_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="232" mass="26244" checksum="456AB2DE1ECB9E4B" modified="2002-06-01" version="1">MREPSRGELEEYREVLEELTGSDLGIADREVTVERIRGPVRADRVFVDGRPVLTVTFDPWTASCVYTLTPHGAREAEEITGERPERRRLVRRDPYGLLDFEPTGTKRDLEKAVTRALEECDVGVLELETATERVNGVELTGLAVYTAREFLYVDVVPPDPAGRPNPVNAVARILHAFGGEETRPWIHPEYRFLGVRRIRDGKVARCRLGRELVEFEARITRAGLVLRPARGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW07</accession>
    <name>Q8TW07_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">slpA</name>
        <name evidence="4" type="ordered locus">MK1231</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1231"/>
    <dbReference type="EnsemblBacteria" id="AAM02444">
        <property type="protein sequence ID" value="AAM02444"/>
        <property type="gene ID" value="MK1231"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1231"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1334"/>
    <dbReference type="HOGENOM" id="CLU_073526_0_0_2"/>
    <dbReference type="OMA" id="IREFHRH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="219" mass="24237" checksum="5FD1773136DFAFCE" modified="2002-06-01" version="1">MAVEKGDFVKIHYTGRVKDTDEVIDTTRKEVAEEHDLNVESGPVVVVVGAGMVWEPVEEALVGAEPGDELEVEVPPEKAFGERDPSLVRTYRESEFRGSVKPGDTVVSPDGRRGRVLSVDGGRVRVDFNHPLAGKALVYEVEVVDVLEDTVERAEGLLETMVPSVDAELELDGNTLRVRVEGDDAANPAWARAKQRFAKLMIEYDDAVEEVVFEERFTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW14</accession>
    <name>RL6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl6</name>
        <name type="ordered locus">MK1224</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW14"/>
    <dbReference type="SMR" id="Q8TW14"/>
    <dbReference type="STRING" id="190192.MK1224"/>
    <dbReference type="EnsemblBacteria" id="AAM02437">
        <property type="protein sequence ID" value="AAM02437"/>
        <property type="gene ID" value="MK1224"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1224"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1327"/>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="OMA" id="VFQDGIY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_L6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_L6_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_L6P_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000260986" description="50S ribosomal protein L6">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <sequence length="204" mass="23378" checksum="638F9ACE19FE69BC" modified="2002-06-01" version="1">MAEAKFEPAKDAIPGVIVMEERVKIKDDVEIEISQREDRRYEVTVKGPKGEVTKEFYYPDVYLWVEDDEVVIAATRSNRRQKAILGMIKAYIENMQKGVTEGHEYKLKLVYSHFPPEVKVDQKEGKVYIENFMGENVPRVAEIVDPENTEVIVQGQDIIVRGIDKEAVGQTAANLEQATYIKDRDPRVFQDGIYIVEKDGKKIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW23</accession>
    <name>Y1214_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0210 protein MK1214</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1214</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0210 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW23"/>
    <dbReference type="SMR" id="Q8TW23"/>
    <dbReference type="EnsemblBacteria" id="AAM02427">
        <property type="protein sequence ID" value="AAM02427"/>
        <property type="gene ID" value="MK1214"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1214"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1316"/>
    <dbReference type="HOGENOM" id="CLU_048704_0_0_2"/>
    <dbReference type="OMA" id="EMAIGMV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08025">
        <property type="entry name" value="RNR_PFL_like_DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01221">
        <property type="entry name" value="UPF0210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="PTHR37560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000070565" description="UPF0210 protein MK1214">
        <location>
            <begin position="1"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01221"/>
        </source>
    </evidence>
    <sequence length="450" mass="47697" checksum="D637DA45101155F9" modified="2002-06-01" version="1">MSSLDVEEVIETIEMIRMRNLDVRAVTLGINLLDRAHPDPEELARDVREKIVEVAGDLVEVVEEVEDELGVPIVNKRIAVTPCSIVAASAVRKEGREAVLTLAEALDEAAEEVGVDYLGGYTALVYDGFTEADEAVLDTIPEAIEGTERLCASVVVADERYGINMDAVYRTAEAVKETAERTDGHGCARLVALTNAPENTPFMAGAFHGVGQPEACVNVGISGPGVVRAVVEELKDVDFRTLHDEIKRTAFKITRVGELVGRRVAERLGVEFGAVDLSLAPTPEEGDSVAEILEGIGLESCGCPGSTAALHLLMDAVKKGGAAATSRHGGYSEAFIPVSEDAGMARAAEEALTLEKLEAMTAVCSVGIDMVVVPGDTPVETIAGIIADEAAIGVVTGKPTAVRIIPAPGKEPGDEFEMGGLLGRAPVMDVSDYRPTMFRRDGRIPPKFPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW31</accession>
    <name>LEUD2_METKA</name>
    <protein>
        <recommendedName>
            <fullName>3-isopropylmalate dehydratase small subunit 2</fullName>
            <ecNumber>4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-IPM isomerase 2</fullName>
            <shortName>IPMI 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Isopropylmalate isomerase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuD2</name>
        <name type="synonym">leuD_2</name>
        <name type="ordered locus">MK1206</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW31"/>
    <dbReference type="SMR" id="Q8TW31"/>
    <dbReference type="STRING" id="190192.MK1206"/>
    <dbReference type="EnsemblBacteria" id="AAM02419">
        <property type="protein sequence ID" value="AAM02419"/>
        <property type="gene ID" value="MK1206"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1206"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1308"/>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="ITPGRYN"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141939" description="3-isopropylmalate dehydratase small subunit 2">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="168" mass="18714" checksum="5C7083095952D9C6" modified="2002-06-01" version="1">MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKNFGCGSSREQAVMALQQAGVACVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVTVDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKNPDKPPWRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW32</accession>
    <name>Q8TW32_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02418">
        <property type="protein sequence ID" value="AAM02418"/>
        <property type="gene ID" value="MK1205"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1205"/>
    <dbReference type="HOGENOM" id="CLU_1792138_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="144" mass="16652" checksum="77AE76A5BF2B54C2" modified="2002-06-01" version="1">MEGLLTIVRRLRARRVVEVGHGRNLRYLKGLLKAGIDAWGVEIDVQHVRRALEEEVPSVNVDAVEKSRWVRRVLRPDLVYAVRPPVELAVGLIERYPTVALRMREEERHELPEPSIQIGDWDLHTVLDLHTFEEDRTVKPQISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW55</accession>
    <name>Q8TW55_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-7 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02394">
        <property type="protein sequence ID" value="AAM02394"/>
        <property type="gene ID" value="MK1181"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1181"/>
    <dbReference type="HOGENOM" id="CLU_741048_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="299" mass="34063" checksum="3E3B02D4C424EF9A" modified="2002-06-01" version="1">MVEWREVPVLPKNTFFKKLNAAIAWIDPFDEFANPDVKELLAGVSDELDPNPLLHWSNVLSAEHPPEEWRIPYELAEYMVCFNKAGAAVLRYFADALRNVFERPSLDRLVRPLAIEMALSDGDELPGLVRSELGIEKGYRGLGKGLPDALRPSRWLLVSVVSHACSNRKARPEEIAEEFEEHLGFELEGPEVIDRLRENVEEAVEDLVTWASREGEDPKPYVEVFREAVTATLRRSIRLVRRPEEIAKSCLKVDPTVSIGGVKIGPEDYDPLWERVRQVDVEEVTAEIVDELLDELTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC7</accession>
    <name>Q8TWC7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD-dependent aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1108"/>
    <dbReference type="EnsemblBacteria" id="AAM02321">
        <property type="protein sequence ID" value="AAM02321"/>
        <property type="gene ID" value="MK1108"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1108"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1163"/>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_2"/>
    <dbReference type="OMA" id="WHKLIEQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="3">
        <location>
            <begin position="11"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="471" mass="51148" checksum="E369538CEC2D7B3D" modified="2002-06-01" version="1">MEYGCLIGGEWLEGDREIVVENPYDGSEVGRVLAPEVDVEALLKDAREGQRRWRERPTYEVREALAEAAHLLKKHEDELAELIALEGGKPIRDARYEVYRTREVLRLSAAEAERLYGETLPGDAQRGRTAELILTVREPVGVVLSITPYNFPLLLPTHKLGPALAARCSVVHKPATVTPLSSLRLAEILLDAGVEPLALQVVVGPGAELGEELARADFDALSFTGSRSVGEHLREISPIPRITLELGGNDPVIVDETADVEAAAEAAVRGACYHAGQVCIAVERAIVVEDVYEEFLEAAVEVAESLKVGDPLDEDTDVGPLIDDGAVEKVRRHVEDAVERGAEILTGGEPEGRLFPPTVLADVPEDALVAREETFGPVLPVIRAKDFEEAIRIANSTDYGLHAAVFTERLDRAVKATRKLEAGGVIVNESTIYRADYMPFGGVKASGVGREGVPQAVREFTEEKTVVIGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD3</accession>
    <name>Q8TWD3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted transcriptional regulator containing a HTH DNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fur_1</name>
        <name evidence="1" type="ordered locus">MK1102</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02315">
        <property type="protein sequence ID" value="AAM02315"/>
        <property type="gene ID" value="MK1102"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1102"/>
    <dbReference type="HOGENOM" id="CLU_1922807_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="131" mass="14867" checksum="475B43421811B578" modified="2002-06-01" version="1">MVPRQHMGLWGLPHLSEDERLRKVKEALKSGRLSLTDAVLATICVAYREFNMGITCDEIVKVLRKLGYNNVNKRKIYSLASRLKKEGLIESNRVSGRAIYAIKDEDKAIERVLGKTRSVKAEDLLKALEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE3</accession>
    <name>Q8TWE3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02305">
        <property type="protein sequence ID" value="AAM02305"/>
        <property type="gene ID" value="MK1092"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1092"/>
    <dbReference type="HOGENOM" id="CLU_2519773_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="84" mass="9260" checksum="25D74954DFF07969" modified="2002-06-01" version="1">MAGKAEILGEVVLCVIYGYRVAGAVSEISGSLSSRWYGHARSTEDLAVRTVEELGRSLGWDPEEVRRWQAIVRTIGDLARRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF3</accession>
    <name>Q8TWF3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-16 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02295">
        <property type="protein sequence ID" value="AAM02295"/>
        <property type="gene ID" value="MK1082"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1082"/>
    <dbReference type="HOGENOM" id="CLU_557379_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="489" mass="53938" checksum="D059759936089B37" modified="2002-06-01" version="1">MAIIRSVGRAASNPVVLIKIVLGASPVLGVIFVLSTLIVLGSTKLAPVSLSLIAAVLTSAPFQVLVSRVILDTLFLETFIPIPGIIILSTIFCTVCSIILYNELTQLFNIKISLATTIATPMATLAYMFTAAHSAVSWARPGLIARRVTMPWALFVISFIVSLMIIEADQAYLVASTILVAMMLLSFIDVIQGVNRISKISIFTRGNLLIKSLKVAIAAFADYSVMMMSAIAYYLVMFFDKFLVWSKYGFPYFPLDSPSFVGMMPLFAGTLAAIKFWDSVEEKLDLLYRVDWDGMYALREFVMKAYWRSLAWTLVLAGLITALIMLAIGISVHRTLGYISLAVLGALSFFAGLGAVKLPRKYAIPLLLTATGALFLKVHVSNISVLMMYLIGSLPGAILVYIYPQLLTFRRDLEALLVTSAVPATELLVWKYFGESFLPMGYLTGAILAAGLSLIFSIRMYWRISREAYRVVAYNAFVSYVTEIPRRWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF7</accession>
    <name>Q8TWF7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metabolic regulator containing V4R domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02291">
        <property type="protein sequence ID" value="AAM02291"/>
        <property type="gene ID" value="MK1078"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1078"/>
    <dbReference type="HOGENOM" id="CLU_1801703_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="143" mass="16300" checksum="CC64BD8E83C4EEC3" modified="2002-06-01" version="1">MRLEDILQMVETCNRKIDRTAFLIARSVIWDLEANGEVRKRAEQLGHEIVKRMEFSDVEDACEYLQEIRCGEVQVGVQEDEADELVADMGYDNVVVYDCISCCGAPEMGETLCAPGERPDPGDDRRHRRPGVLRDRVRMLGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH0</accession>
    <name>Q8TWH0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gltB_3</name>
        <name evidence="2" type="ordered locus">MK1064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1064"/>
    <dbReference type="EnsemblBacteria" id="AAM02277">
        <property type="protein sequence ID" value="AAM02277"/>
        <property type="gene ID" value="MK1064"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1064"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1118"/>
    <dbReference type="HOGENOM" id="CLU_078510_1_0_2"/>
    <dbReference type="OMA" id="PYIEEFC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012061">
        <property type="entry name" value="Glu_synth_lsu_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006519">
        <property type="entry name" value="GOGAT_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="1">
        <location>
            <begin position="29"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="230" mass="25041" checksum="E7F4BC894ACEB570" modified="2002-06-01" version="1">MKERVIDCRDKEPRDINSALKTYAREYDRIVLENPGAKHYIAAGLTEEVEVIIKGSVGYYVGTMIHGPRILVKGNAGWYPGDNVTKGEIVIEGHAGDGVGQGMYGGTIVVRGDAGSRVGQIMKNGTVIVGGDVEIMTGMYMMGGTIIVLGDAGTYTGESMLRGEIYVLGEVEDLGKNAEAVDPNGEDIERVWELVRAYDFDVTRDDLKALTKIVPRSKRPFYGSEEMEEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH2</accession>
    <name>PDXT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">MK1062</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWH2"/>
    <dbReference type="SMR" id="Q8TWH2"/>
    <dbReference type="STRING" id="190192.MK1062"/>
    <dbReference type="EnsemblBacteria" id="AAM02275">
        <property type="protein sequence ID" value="AAM02275"/>
        <property type="gene ID" value="MK1062"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1062"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1116"/>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="OMA" id="VFIRAPI"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135681" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <sequence length="202" mass="22377" checksum="F969BC155093CD0C" modified="2002-06-01" version="1">MKVAVVAVQGAVEEHESILEAAGERIGEDVEVVWARYPEDLEDVDAVVIPGGESTTIGRLMERHDLVKPLLELAESDTPILGTCAGMVILAREVVPQAHPGTEVEIEQPLLGLMDVRVVRNAFGRQRESFEVDIEIEGLEDRFRAVFIRAPAVDEVLSDDVKVLAEYGDYIVAVEQDHLLATAFHPELTDDPRLHAYFLEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH7</accession>
    <name>Q8TWH7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02270">
        <property type="protein sequence ID" value="AAM02270"/>
        <property type="gene ID" value="MK1057"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1057"/>
    <dbReference type="HOGENOM" id="CLU_1187786_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="233" mass="26556" checksum="8527F118A9BB4439" modified="2002-06-01" version="1">MSLVAARNPPDPRKLGSFKVRRCAACGRLMRIELSECNCVRCNDLRGEPLEEGPYIENSGPYCDRCRGGVEPTSRLPEVAVAVCFLTDRRNHDVLYRAAGALAEVVPRLREERRFVAVGTPVDDLYFNLRCGMEAGIDFGIRAQMSSIFTHMGPEYLVIAHNPVRKLRGRPRERFRYTLESNIEELRRVIVLEDVVRNTDFKSVYRDLGREYPDVFVFASSGEVEKLVDALRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI3</accession>
    <name>Q8TWI3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted nucleotide-binding protein related to universal stress protein, UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02264">
        <property type="protein sequence ID" value="AAM02264"/>
        <property type="gene ID" value="MK1051"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1051"/>
    <dbReference type="HOGENOM" id="CLU_1943866_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="129" mass="14476" checksum="3A8B081EDCD154A5" modified="2002-06-01" version="1">MTRYVVLLDGSEASFEALEHALEEADEVIGVYLIDCRAFKGLNEDMARALEEFMRQEAELIRETVEKRGAEFEIVRGEGPEAVMEYAREVGADAVVVGVGHIPRFEDITFRRGIEGHVRDVPVVLVPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK8</accession>
    <name>Q8TWK8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator of amino acid metabolism consisting of an ACT domain and a DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02238">
        <property type="protein sequence ID" value="AAM02238"/>
        <property type="gene ID" value="MK1025"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1025"/>
    <dbReference type="HOGENOM" id="CLU_1792142_0_0_2"/>
    <dbReference type="OMA" id="IWLVIEN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="98"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="18764" checksum="AF0AF626EEB1AA30" modified="2002-06-01" version="1">MCKYRKVLGVSRSRLSGRARDILELVVNNSGITVSEISRRLGLHRSTLDRYVKILKDLGLVETKPGRGGGVYPTKDAVLLVRSGGRVTIVRRGGGRARILIEAEDRPGLLADVTNRLASAGVNILETELKVEEGIAIMEFEAENVVHEEVLQELDGLSGLIRVEVEPRGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL2</accession>
    <name>Q8TWL2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sugar phosphate isomerase/epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1021"/>
    <dbReference type="EnsemblBacteria" id="AAM02234">
        <property type="protein sequence ID" value="AAM02234"/>
        <property type="gene ID" value="MK1021"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1021"/>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="OMA" id="PTIDLNP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="1">
        <location>
            <begin position="49"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="27816" checksum="C214039F54F129F3" modified="2002-06-01" version="1">MTVLVSTLALGDWWADPERVNELPELTGSDGVELILEGSFQPGRLDPNELNVEVSSVHAPFADLNPASPSDHHHEYILEVIQRAAELAAELDAHYLTVHPGHLTPVTIHDRELAIELAIETLGELADEVRSFGVEPLVENMPDHSLLLGTSADEMEEILRASGCGFTLDVGHALTAEGSLRPYLRLRPDLLHVHDNSGDGDEHLPPGSGILDFEELRRALHHRVLPVVEVRGIEKAREAVRTVREIMERPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN2</accession>
    <name>Q8TWN2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">MK1000</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1000"/>
    <dbReference type="EnsemblBacteria" id="AAM02213">
        <property type="protein sequence ID" value="AAM02213"/>
        <property type="gene ID" value="MK1000"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1000"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1048"/>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="OMA" id="GYTNFRE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO" evidence="12">
        <location>
            <begin position="70"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAM02213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="293" mass="34320" checksum="07613146B8E1919C" modified="2002-06-01" version="1">MVMVERVVQALPEMEPEDFKVLRALELEMRRHDWVPFDRLLERTGLDEKELGYRLSRLDRWDMVVRTRRQTVGYLGYQLRPEGYDALALRALVDQGVLEGLGPEIGVGKEADVYLGISPKGAQLAVKFNRIGRTSYTKIKRYREYVKDKRHISWLYVNRLTAEREFEALLHLYPEGVSVPRPVAQNRHVLVMERFEGRELAETRVENPEAVLNRVLEEYEHALEVGVVHGDLSQFNIVVEGDDVLLIDWAHWVEVSHPSARELVERDVRNVCDYFRRKYGVHRHPREFFENSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP5</accession>
    <name>Q8TWP5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S protein related to pyruvate formate-lyase activating enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0987"/>
    <dbReference type="EnsemblBacteria" id="AAM02200">
        <property type="protein sequence ID" value="AAM02200"/>
        <property type="gene ID" value="MK0987"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0987"/>
    <dbReference type="HOGENOM" id="CLU_053467_0_0_2"/>
    <dbReference type="OMA" id="DCFYCPL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="PTHR43288:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="12"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="40013" checksum="FC5C0B223595E8A2" modified="2002-06-01" version="1">MVRGAFPKGCRLCVCGAKLVVFVTGVCNRGCWYCPVSPRKKGSDVSFANERPIRSERDLVKEAELMDAEGASLTGGDPLTRLERAVNIIRTLKDEFGDDFHIHLYAPAESVREGAIEELDGVGLDELRVHPSPDSTVNRRAAEVLEDSGMDLGFEMPAIPGEENWILEVAKLADEYGFDFLNVNELEFTESNAEELRRRRFERVDDDLSDAVAGSERTALTALSEVANDVGITLHYCPSEVKDAVQFRKRIKRMAKNVARDHEEIDEEGLIVKGIFEVISGDPEDLADVLINILEVPEEWVYVDGSRVETKPFVVDELADVLSDLEEASRCEIRAWIIREYPTWDRTVVERWPVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS8</accession>
    <name>Q8TWS8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ordered locus">MK0954</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0954"/>
    <dbReference type="EnsemblBacteria" id="AAM02167">
        <property type="protein sequence ID" value="AAM02167"/>
        <property type="gene ID" value="MK0954"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0954"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.999"/>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="OMA" id="YMRRHGP"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="PTHR38341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="SSF111249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="region of interest" description="FBP binding" evidence="15">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="FBP binding; shared with dimeric partner" evidence="15">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AAM02167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="375" mass="41620" checksum="3A9F8E7BB626464C" modified="2002-06-01" version="1">MVSVAEEKVTVSVIKADVGGFPGHSEVHPDLLEACEGVLEDAVDEVIIDYYVTRCGDDIDLIMTHTRGEDDEKVHELAWNAFQEATKVAEDLKLYGAGQDLLSDAFSGNVRGLGPGAAEMELVERPSEPIIVFCCDKTEPSAFNLPLFRIFADPNNTAGLVLDPSMHDGFEFEVHDVIDQKKVILKCPEEMYDLLALIGQTQRYAIKRVYKKGDGDEAERIAAVTSTERLNLIAGEYVGKDDPVAIVRCQSGFPAVGEVLEPFTFPHLVAGWMRGSHNGPLMPVSEEEAHPTRFDGPPRIIALGFQLRNGELVGPQDLFADPAFDRAREIANEVADYIRRHGPFQPHLLSEEELEYTTLPDVLKKLEDRFEDLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV0</accession>
    <name>Q8TWV0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0931"/>
    <dbReference type="KEGG" id="mka:MK0931"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.973"/>
    <dbReference type="HOGENOM" id="CLU_044556_1_0_2"/>
    <dbReference type="OMA" id="HGIASCE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1 2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0712">Selenocysteine</keyword>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="416" mass="46868" checksum="F780CFE8269C821D" modified="2008-02-26" version="3">MAEGAVEIQPTTRHEGHAKLVLYVDDEGYVERAFYLNTSAVRGFEALAKGRPAEFVQVAVMRICGICQATHGTASAEAFERAMGIEPPKDGKLLRELCALGNRIQSHVLHQLLVLDDFVEDESEKVEAVKRIQQIRRIGQYVVDVVGGEGIHPPNIRIGGMAENISEAARRKLYRRLREARELMMEQHEFMVNIVERFGDENDLDIDEFGRHDQPFLATHPTYGDPDRLDMDRVVELLPIEYYGEEHKEVAYQHRGQIPLYDGVPVEVGPRARYILFDGVDPRGVLYIHVLRSQETLAAIDRAMTILDELNTSGKTLAEWEPKAGVGIGVHEAPRGTNVHIAKVNEKGIVEDYRIIAASTWNFPVVEKAIEGENEEYAEVIMRCYDIUASCAAHVVKEVRDADSREKIRESVVKLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-07-05" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY3</accession>
    <name>COFG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="1">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofG</name>
        <name type="ordered locus">MK0898</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02111" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02111.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWY3"/>
    <dbReference type="SMR" id="Q8TWY3"/>
    <dbReference type="STRING" id="190192.MK0898"/>
    <dbReference type="EnsemblBacteria" id="AAM02111">
        <property type="protein sequence ID" value="AAM02111"/>
        <property type="gene ID" value="MK0898"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0898"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.940"/>
    <dbReference type="HOGENOM" id="CLU_054174_0_0_2"/>
    <dbReference type="OMA" id="TTACRYT"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016841">
        <property type="term" value="F:ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044271">
        <property type="term" value="P:cellular nitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018130">
        <property type="term" value="P:heterocycle biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901362">
        <property type="term" value="P:organic cyclic compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000147765" description="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="334" mass="38797" checksum="607E8317C9553058" modified="2009-03-03" version="2">MVSYSKNVFVPLTRLCRNRCAYCTFRREPEEVRSPYLSPEEVFEIVEKGKEAGCKEVLFTFGERPEERYDEALEWLEEHGYSSTVEYLVDLCRRCVEEYDMLPHSNPGVITKREMRKLRRWNASMGLMMEILSERLCEESGPHEHSPGKRPEERLKVLKYAGELKVPFTTGILIGIGETWEERVKTLEEIQRMHERYGHVQEVIVQNFRTKPGIPMEDHPEPTPADLLRTVATARLILPDVPVQVPPNLNRETGQLALLAGANDWGGVSPVTKDYVNPEAPWPEIEELKRLTEDVGFRLRERLPIYPEYVRRGWYHANISEVVERLSDDEGFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX22</accession>
    <name>Q8TX22_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02070">
        <property type="protein sequence ID" value="AAM02070"/>
        <property type="gene ID" value="MK0857"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0857"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.900"/>
    <dbReference type="HOGENOM" id="CLU_1025314_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="271" mass="31160" checksum="FD82CDBEF83B6583" modified="2002-06-01" version="1">MWIRRYVPAKALIVVIITTLTVPYFVFYGKPRGPGYPTELYGYRIVLEDPALAVVDVEPRRTLLFRHTLTVSDEGWSVHPVFDIPPLIFVRAERMDTLETVKRNLWAVVLQEGAGRVFMPDEFVEGGVPGLITGIAGQAWAWKHLKWAREFGVIHGLRRYAKEVGPERISTAILEEYRRAPWRAILDFCDMMKNSDVGRFLRSRGLSFRDFLTSKDPKLVAEVLVVLDLGRLDERVVDERIRYVLDARAYPIPESEEKIVDKAARELLRHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX26</accession>
    <name>Q8TX26_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0852</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02065">
        <property type="protein sequence ID" value="AAM02065"/>
        <property type="gene ID" value="MK0852"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0852"/>
    <dbReference type="HOGENOM" id="CLU_1340740_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="21635" checksum="3A57C73A5BB9D153" modified="2002-06-01" version="1">MRTKIVAVLLISAIFIGALMGVITYQWSISSKGARVGCYHVAITNVKGSVEPGDVKRALLKCPYVIRVGGIRTAPSSCDAYIIVKAPTPVSKYDIAQELSQALRKAGLGKAPAVFLNSVNAYVVEVAVEDPKIPASVAARKVASAPTILAIFKVERRGSVYVFEVASLEEEYPVAYSVTGALKLAGLKPVGVANVTPVKWGYVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX44</accession>
    <name>ILVC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="ordered locus">MK0832</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX44"/>
    <dbReference type="SMR" id="Q8TX44"/>
    <dbReference type="STRING" id="190192.MK0832"/>
    <dbReference type="EnsemblBacteria" id="AAM02045">
        <property type="protein sequence ID" value="AAM02045"/>
        <property type="gene ID" value="MK0832"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0832"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.875"/>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151392" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="183"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="25"/>
            <end position="28"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <sequence length="326" mass="36460" checksum="BB8F208D79EA7128" modified="2002-06-01" version="1">MAKIYGDEDASLEPLEDKTVAVIGYGSQGEAQAKNLRDSGIDVIIGVREGGPSWERAKKDGFEVLPIPEAAEAGDVVHILIPDEVQPQVYREHIHDNLEKGNALGFSHAYNIHYGLIEPPEYVDVILVAPKGPGHMVRKLYTEGFGTPALVAVHQDYTGEAMDLALAMAKAMGFTRAGVIKTTFREEVETDLFGEQVDLVGGVLYMILYAFETLVEAGYQPEVAYFETLHELKLIVDLIYEKGLSGMLDNVSNTAEYGGLTRGKRIINKEEMRKVLEEIRSGEFAREWTLENESGRIVMKRLREEIENHEIEKVGERLRKMMGFED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX50</accession>
    <name>RL10_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10</name>
        <name evidence="1" type="synonym">rplP0</name>
        <name type="ordered locus">MK0826</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02039" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02039.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX50"/>
    <dbReference type="SMR" id="Q8TX50"/>
    <dbReference type="STRING" id="190192.MK0826"/>
    <dbReference type="PRIDE" id="Q8TX50"/>
    <dbReference type="EnsemblBacteria" id="AAM02039">
        <property type="protein sequence ID" value="AAM02039"/>
        <property type="gene ID" value="MK0826"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0826"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.868"/>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="OMA" id="MAHVAEW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_L10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="50S_L10_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="RL10_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="RL10P_insert"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154794" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="323"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="357" mass="39250" checksum="470294320ADBBE5C" modified="2002-08-13" version="2">MAVKAKGQPPSGYEPKVAEWKRREVKELKELMDEYENVGLVDLEGIPAPQLQEIRAKLRERDTIIRMSRNTLMRIALEEKLDERPELEPLLDYIEGPVAFIFTNLDPFKLYKLLEESKASAPAKPGDIAPEDIVVPEGPTPFEPGPIVSELQQAGLPAQIQDGKVVITKDTVLVKEGEEIDEKTAEILKKLEIEPMEVGVDIVAIVAEGTLFERDDLAIDFDEYEDMAKEAAQHAFNLSINAAIPTAETADVIVAKAHTEALNLAVNAGVPVPDETVMGCILAKAHGEMLALAGAIAEVDEEALDEELLEMVSRSAEAAERKEKEEEEEEEAEEEEAEEEEEEEEEEAAAGLGALFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX67</accession>
    <name>NNR_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional NAD(P)H-hydrate repair enzyme Nnr</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName>ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber>4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>NAD(P)H-hydrate epimerase</fullName>
                <ecNumber>5.1.99.6</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>NAD(P)HX epimerase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="primary">nnr</name>
        <name type="ordered locus">MK0808</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136"/>
    <dbReference type="EC" id="5.1.99.6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX67"/>
    <dbReference type="SMR" id="Q8TX67"/>
    <dbReference type="STRING" id="190192.MK0808"/>
    <dbReference type="EnsemblBacteria" id="AAM02022">
        <property type="protein sequence ID" value="AAM02022"/>
        <property type="gene ID" value="MK0808"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0808"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.849"/>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416429" description="Bifunctional NAD(P)H-hydrate repair enzyme Nnr">
        <location>
            <begin position="1"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal">
        <location>
            <begin position="8"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal">
        <location>
            <begin position="217"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="NAD(P)H-hydrate epimerase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 1; for epimerase activity" evidence="1">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 1; for epimerase activity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP-dependent (S)-NAD(P)H-hydrate dehydratase" evidence="1">
        <location>
            <begin position="217"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 2; for dehydratase activity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>1</label>
            <note>for epimerase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>2</label>
            <note>for dehydratase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="397"/>
            <end position="401"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>2</label>
            <note>for dehydratase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="499" mass="53849" checksum="BBCAEB974F16C75D" modified="2002-06-01" version="1">MFITSKEMRRIELNSRWLGFEEDFMMENAGAGVARVVIGEYSPNDVLVVCGTGGNGGDGFVTARHLDSEGVDVDVLLVGRREAIKNEAAELNLRRLDRAGIPVQEVRDSEDLESVDFERDVVVDALLGFGIRGRLREPVRSAVLRINEASRAGTRVVSIDIPTGLDPDSGETPDVAVEADLVVSIHRHKRGVRKLRDVFLRRVNAGIPEIAERICGPGDLITSDIWRRDPWSHKGQHGRVLIIGGSRKYVGAPQLAARGALRAGVDLVFLLTVDAVPKNDPNVIYRAVPAERLEPEHLDEVDLEGVDTVVVGPGLGADADSVGILRELAESFDGMIIVDADGLRGISGVNVDDRFVLTPHAGEFRREFGEELGRSLEDRSEAVRRVSEELGCTILLKGRVDVIGSPDGEIRWNVTGTPAMTVGGTGDVLAGVVAGVAARCREGFEAACIGAFVVGSAGCLAERRLSQGLTAEDVAEYVPKVLRNPWAAEPEAVTEVRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA3</accession>
    <name>Q8TXA3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbonic anhydrases/acetyltransferase homolog, isoleucine patch superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0772</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0772"/>
    <dbReference type="EnsemblBacteria" id="AAM01986">
        <property type="protein sequence ID" value="AAM01986"/>
        <property type="gene ID" value="MK0772"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0772"/>
    <dbReference type="HOGENOM" id="CLU_887429_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="KH type-2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="313" mass="35283" checksum="9E2759DF78F6F907" modified="2002-06-01" version="1">MGIEVKEIRPGRGRPELVYARAELVVHKESLKATLIGREGSTIREIGKRARELLEERVGKPFYLDLTVVVDPKRSPTNRWFKVRDSSEAWLCPAFKHPHLVSVRESRIRGNARVAAFVELEHVTVHGGAMISNFSSIRRSVLHKRVSVSSHCEIERSELGPVTFVGDGAEIHGCKIEERCFVGMNARLSRCEIGRWSVVGAGAHISDSRVPERSLVVGNSIVDRLDVYTLVVGKRLKLELNGGEIWSGQGEKYYVCYLPEDNRITVRPEPTERVLCEVRKANDKWEVRGEGFVRTVRGSSLHWYVGHERGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB8</accession>
    <name>Q8TXB8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0756</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01970">
        <property type="protein sequence ID" value="AAM01970"/>
        <property type="gene ID" value="MK0756"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0756"/>
    <dbReference type="HOGENOM" id="CLU_830581_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="334" mass="37962" checksum="D959CFF68C204CB5" modified="2002-06-01" version="1">MTSPPSSIALSGGKDSVATLIDAADRWNVKVAVTVVHEFTDKTCLENARKAAEHVGVDHEVVKLRLREWFARALKEGRPICTKCGRSVLTAACLRARELGCATVLTGHELRGKFGRRTTHPYPPGVTVVRYPALRRWTWEDIRDIVSSLGWFNPEYTCPIRPYGVHRFIEEVGYNPLTGRACTIMLEGVATPQEALAYLRETETPEEDPERVLDILGLEEKAAFPNGIPDGPRDEDEIIRDLTYRLLMYARQALRSLVRGTLNRVERRERFTQLYFVMSEYGKIDDRVEHLDIEAFVRLATGDREEAERLLRDVKKLMRSILAEYGRTIPGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD6</accession>
    <name>Q8TXD6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speB</name>
        <name evidence="6" type="ordered locus">MK0738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0738"/>
    <dbReference type="EnsemblBacteria" id="AAM01952">
        <property type="protein sequence ID" value="AAM01952"/>
        <property type="gene ID" value="MK0738"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0738"/>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="28751" checksum="A31A9E721C889EBE" modified="2002-06-01" version="1">MWESRRKSEPRFPLSFSSDDVKSPDVAVVGLPRERVLPEYPTGQAEAPNAVRSAASTLDFYDPEVGDPLEILTLVDEGNLRTFEPESEPEIFVVLGGDHSITPEIVQELTPRRLLWLDAHPDLRRSERHDGALRGCLEIVDEVFLVGVRCWSREEYDVFREHEHVVSVDHGDITEVIDDVDYVSLDLDVLDPSVVPGVTTPEPGGLPYRLCADILRWAGRKRAHLDVVELCPTVESHVSPVTAARLVGEYLKGVAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG5</accession>
    <name>Q8TXG5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">16S rRNA G1207 methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rsmC</name>
        <name evidence="2" type="ordered locus">MK0709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0709"/>
    <dbReference type="EnsemblBacteria" id="AAM01923">
        <property type="protein sequence ID" value="AAM01923"/>
        <property type="gene ID" value="MK0709"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0709"/>
    <dbReference type="HOGENOM" id="CLU_018398_7_2_2"/>
    <dbReference type="OMA" id="HMIDVNE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MTS" evidence="1">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="199" mass="22341" checksum="A7268A04FE5FF203" modified="2002-06-01" version="1">MRAPYSGRGKPSREELGMIIARIRGREYRFLTAPGVFSWRRIDPGTQLLAENMDLEGVHSVLDLGCGYGVLGIVAAKELGEGHVVMTDVNRRAIWLANENRRLNDVEDITEVREGSLYDPVEDEEFDRIVSNPPIREGLDLVLRIVREAPNHLTEDGELWLVVRRKMGSKRILSEMRTVFGSAEVAARGGGYWVLRAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH6</accession>
    <name>Q8TXH6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01912">
        <property type="protein sequence ID" value="AAM01912"/>
        <property type="gene ID" value="MK0698"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0698"/>
    <dbReference type="HOGENOM" id="CLU_482037_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="565" mass="62979" checksum="3944F15638CFF7BF" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS6</accession>
    <name>Q8TXS6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0584</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01799">
        <property type="protein sequence ID" value="AAM01799"/>
        <property type="gene ID" value="MK0584"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0584"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.619"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="242" mass="28085" checksum="D0864CDC2C948126" modified="2002-06-01" version="1">MNVRPGGPPVLDPVRLGVPMEVPEDPVELVRVFRVLVETLERGTLPFLGASYRSVNGKVYGPYYEARWKPRKGERGRTIYLGREDNESVQFLREWLETIRLAAPRLSEHRKAKWFVARAVRRALVPVLRQLAQMSAEHRAEVLQKLEEIVKEGVSRTCSILRSVEFNPLRRLGWPGSGKEIIDSVLRGLPGTVRDILVDVLAPWPAWYSLRIVRLWRGEREARRYWRERVGLYDRVKQREAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT7</accession>
    <name>HDRC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoB--CoM heterodisulfide reductase iron-sulfur subunit C</fullName>
            <ecNumber evidence="3">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrC</name>
        <name type="ordered locus">MK0573</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXT7"/>
    <dbReference type="SMR" id="Q8TXT7"/>
    <dbReference type="STRING" id="190192.MK0573"/>
    <dbReference type="EnsemblBacteria" id="AAM01788">
        <property type="protein sequence ID" value="AAM01788"/>
        <property type="gene ID" value="MK0573"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0573"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.608"/>
    <dbReference type="HOGENOM" id="CLU_121273_0_0_2"/>
    <dbReference type="OMA" id="DPDLWLC"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000150075" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit C">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY39"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="192" mass="22022" checksum="2645D1648F85F4DB" modified="2002-06-01" version="1">MVEPRDTVIREEDLNPDFLEELSELVEPVFEEEEVLSVQACYQCGTCTGSCPSGRRTSYRTRLIMRKLQLGLVDEVIKSDELWMCTTCYTCYERCPRGVKIVDAVKAARNLAAKKGYMAKAHRMVAMFVIKTGHAVPINDEIREVRKNIGLDEVPPTTHRYEEALEEVQKLVKINEFDKLIGYDWEEGDLVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY02</accession>
    <name>CPGS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cyclic 2,3-diphosphoglycerate synthetase</fullName>
            <shortName evidence="1">cDPGS</shortName>
            <ecNumber evidence="1">6.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cpgS</name>
        <name type="ordered locus">MK0505</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-bisphosphoglycerate + ATP + H(+) = ADP + cyclic (2R)-2,3-bisphosphoglycerate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:79081"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.5.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic 2,3-diphosphoglycerate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY02"/>
    <dbReference type="STRING" id="190192.MK0505"/>
    <dbReference type="EnsemblBacteria" id="AAM01720">
        <property type="protein sequence ID" value="AAM01720"/>
        <property type="gene ID" value="MK0505"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0505"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.536"/>
    <dbReference type="HOGENOM" id="CLU_638764_0_0_2"/>
    <dbReference type="OMA" id="RRCGGGM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036356">
        <property type="term" value="F:cyclic 2,3-diphosphoglycerate synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01908">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016557">
        <property type="entry name" value="Cyc_diphosphoglycerate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009445">
        <property type="entry name" value="Cyc_PG_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000313691" description="Cyclic 2,3-diphosphoglycerate synthetase">
        <location>
            <begin position="1"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01908"/>
        </source>
    </evidence>
    <sequence length="456" mass="49620" checksum="0957551F80576226" modified="2002-06-01" version="1">MTAVKRILALVDGEHYIPVTREALETVEELDLGELVGAVFIGGTEKISEPEAVKRELGVRVWLSESEDEIPVDMIVKVIEEEDVDVVLDLSDEPVVSPDNRFEIASAVLSAGAEYWCPDLRLKPVEFHDVLEKPSLRIIGTGKRVGKTAVSAYTCRVLNARGYNPCVVVMGRGGPREPEIVRGDEIELTPEYLLKEAEKGKHAASDHWEDALLSRIPTVGCRRCAGGLAGRTFTTNIVRGAKIANELPADFVVVEGSGAAVPPIKTDAGIVIVGANQPLEHIGGYLGPYRIRMCDLAIITMCEEPMADDAKIRKVERTVREAGDGIEVVLSVFRPKPTEDVEGKRAMFVTTAPEEVVSRLVEHLEEEYGCEIVGTSPHLSNRPKLRKDLEKYIDDADILLTELKAAAVDVATREALKAGLGVVYVDNVPIAVGGDYDHVGDAVENVAELAIDRFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY16</accession>
    <name>Q8TY16_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase, probably involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01705">
        <property type="protein sequence ID" value="AAM01705"/>
        <property type="gene ID" value="MK0490"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0490"/>
    <dbReference type="HOGENOM" id="CLU_825413_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="336" mass="37674" checksum="DF31D949BB66D958" modified="2002-06-01" version="1">MERIVVLIPAKHESVEAVEKPLRSVLSQKGVEIEKVVIAAGTEDDHRRFSRRFADDDIVEVVKAGGNVKGETVNNALKRVSARADYVFLIDAGDELGSDRYIRELLEEDATLAFGRIRYCGRNLTGLMVGLQFDVVSSGISFWGNVVGSAPVFTTGTLFKATFLLEEGLPENLAEDVTLGLIHTWRKVGFVYRPDLEVWMDDPASLKENFFQQSRWWAGMYQACAEALRSRNLPGIGFAVFVLGSLLASFLTTYILPLVYPWTILISLAGRMIYSVLAALECSNRRGPLWALAVMPCQFMWTFFVEWAAVYGLIWLAIRGNVWYRTTRASEGDDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY17</accession>
    <name>Q8TY17_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0489"/>
    <dbReference type="EnsemblBacteria" id="AAM01704">
        <property type="protein sequence ID" value="AAM01704"/>
        <property type="gene ID" value="MK0489"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0489"/>
    <dbReference type="HOGENOM" id="CLU_049311_3_3_2"/>
    <dbReference type="OMA" id="EIVLCNA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="PTHR12993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="SSF102588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="22684" checksum="C8C908371D57294C" modified="2002-06-01" version="1">MIVMTVFAHPDDMEVHCGGTVRKLSSAGHRVIEVIMSKGNNGGDEEVREREAIEGAKCLGVDRVEFLNHEDGRISVDPESIDAVRALISKFEPDVVFTHSPNDTHQDHRRTFRIVTSAVSKFPEVTVLMGEGPSTVGFQPVVYVDVTDVLEKKLEAVRVHRSQVERGAISEEMVMKTAEFRGMEIGVKYAEAFEAFRISGSLLLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY18</accession>
    <name>Y488_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein MK0488</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0488</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY18"/>
    <dbReference type="SMR" id="Q8TY18"/>
    <dbReference type="STRING" id="190192.MK0488"/>
    <dbReference type="EnsemblBacteria" id="AAM01703">
        <property type="protein sequence ID" value="AAM01703"/>
        <property type="gene ID" value="MK0488"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0488"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.518"/>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="OMA" id="RGCIGFP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="PTHR13016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="SSF143447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00296">
        <property type="entry name" value="TIGR00296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142378" description="Protein MK0488">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <sequence length="207" mass="23458" checksum="503EC418832A94A7" modified="2002-06-01" version="1">MGEKLTLEEGEFLVRLARKAIVHYLESGKKIEEKPPTQRLAEKRGVFVTLKKYPDDELRGCIGFPEPIKPLVEATVEAAISAATGDPRFPPMRDPSEMEEIKIEVSVLTPPKKLEVDNPKEYVEKIEIGRHGIIVRRGARSGLLLPQVPVEEGWDEIEFLSHACLKAGLPPDWWCSPDCEIYVFEAQVFEEEEPEGPVRERDLAEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY20</accession>
    <name>Q8TY20_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATPase of the AAA+ class</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0486</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2021" name="J. Am. Chem. Soc." volume="143" first="15998" last="16006">
            <title>Seven Amino Acid Types Suffice to Create the Core Fold of RNA Polymerase.</title>
            <authorList>
                <person name="Yagi S."/>
                <person name="Padhi A.K."/>
                <person name="Vucinic J."/>
                <person name="Barbe S."/>
                <person name="Schiex T."/>
                <person name="Nakagawa R."/>
                <person name="Simoncini D."/>
                <person name="Zhang K.Y.J."/>
                <person name="Tagami S."/>
            </authorList>
            <dbReference type="PubMed" id="34559526"/>
            <dbReference type="DOI" id="10.1021/jacs.1c05367"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 1-90</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="7DG7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-90"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TY20"/>
    <dbReference type="STRING" id="190192.MK0486"/>
    <dbReference type="EnsemblBacteria" id="AAM01701">
        <property type="protein sequence ID" value="AAM01701"/>
        <property type="gene ID" value="MK0486"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0486"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.516"/>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="OMA" id="DFVEWLH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0404">Intron homing</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="7">
        <location>
            <begin position="762"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="INTEIN_C_TER" evidence="6">
        <location>
            <begin position="1005"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1173"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="7DG7"/>
        </source>
    </evidence>
    <sequence length="1249" mass="140983" checksum="2C75ED70478F7C74" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY36</accession>
    <name>Q8TY36_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaH</name>
        <name evidence="2" type="ordered locus">MK0470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0470"/>
    <dbReference type="EnsemblBacteria" id="AAM01685">
        <property type="protein sequence ID" value="AAM01685"/>
        <property type="gene ID" value="MK0470"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0470"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.500"/>
    <dbReference type="HOGENOM" id="CLU_1253609_0_0_2"/>
    <dbReference type="OMA" id="EMFRTPG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017059">
        <property type="entry name" value="NiFe-hyd_3_EhaH_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10125">
        <property type="entry name" value="NADHdeh_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036536">
        <property type="entry name" value="EhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="223" mass="23938" checksum="D7DA536F3B153FF1" modified="2002-06-01" version="1">MYPSGKVLFIIPLGDIVPYLSTINMIILAVLLAVLALPILSNTDVYVEIDHRRPRIKAKIVSPREDKIKAALALVSTLAATGVVTTGDVFNFTLFLSLLGISNMGLIGTTVEDEFALECAFNYGLMCLIASLPLFGGAALILASTGTLSVHELMKMPKGAGGWTLTYGKALLTAGVIGEAGVGPFYAVKVDSFRRIWGRYAFVIHLTTLLTFSRYLELLAALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY38</accession>
    <name>Q8TY38_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Membrane protein related to formate hydrogenlyase subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ehaJ</name>
        <name evidence="6" type="ordered locus">MK0468</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0468"/>
    <dbReference type="EnsemblBacteria" id="AAM01683">
        <property type="protein sequence ID" value="AAM01683"/>
        <property type="gene ID" value="MK0468"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0468"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.498"/>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="OMA" id="KIMARIQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="283" mass="31180" checksum="8F0DDB4F112F0239" modified="2002-06-01" version="1">MIVLETLVGITEAFLVGSVFLGLHRKVMARIQRRPGPPIVQEFLHTLKFMLKEGYAPLTSAEFLYWMVPVFNVIIWSAAVTISAVYRGNLLPFFALYASYKVLSHGAGVSSGSTYTKIGGVRHAIMPAGELALACSLISAYFVTGHMDVTGILAWEHAHGPLIEHIPFSALAFFTLLWVDSPYSPLDPSKGYDIVEGYLTEYPGFLRGLMYVAEAIKYYCELWVFQAVFLGISDPVSHLLTMGVLTLLLASMCALTPILNPYQAVGFHVIIASMMFLDFLVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY43</accession>
    <name>Q8TY43_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ni,Fe-hydrogenase III large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ehaO</name>
        <name evidence="3" type="ordered locus">MK0463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0463"/>
    <dbReference type="EnsemblBacteria" id="AAM01678">
        <property type="protein sequence ID" value="AAM01678"/>
        <property type="gene ID" value="MK0463"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0463"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.493"/>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="OMA" id="TFAECIE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="143"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="304"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="409" mass="46194" checksum="43DEF878377A5341" modified="2002-06-01" version="1">MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEMGYCHRGIEKIMEGMPWQKAAFLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVLVQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDCIEEITGNRILTGWNVVGGVRMDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQARASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEGRHRRELEVNAGHVDNRVEAPRGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSPSIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKAREVIRACSRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY59</accession>
    <name>Q8TY59_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01662">
        <property type="protein sequence ID" value="AAM01662"/>
        <property type="gene ID" value="MK0447"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0447"/>
    <dbReference type="HOGENOM" id="CLU_854214_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35553" checksum="8429065EE4CF49CC" modified="2002-06-01" version="1">MSTDYLPIGSHEVIRALHDLEPDVVAYLPGFPLNEVVRALEESDHPFEVVPVASEFDAVGIVIGLAQADGYGVAVLKDKGAYVAAQLLAEEGDWPGLLIIGLDADGRGSYTCSVELPDVLRDLKLRVEIPETQDDIYETILDAAESSRDEHRLACVVLTEDLLLSSHPPGKPEVEPTGEADWDELLRAIDFYDTVAVVVGKGVLGDISRDLPAFTSSLNDDRDALDRLVEILESLGLDVRLYCTKHASKFLPGIPPTGVNTGNVVEEELLVLVGASYDTFAVDFRSDFVVSVNPDPDAYAHRVADARFVMTLSDFVRELEYRSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY85</accession>
    <name>NOP10_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">nop10</name>
        <name type="ordered locus">MK0420</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY85"/>
    <dbReference type="SMR" id="Q8TY85"/>
    <dbReference type="STRING" id="190192.MK0420"/>
    <dbReference type="PRIDE" id="Q8TY85"/>
    <dbReference type="EnsemblBacteria" id="AAM01635">
        <property type="protein sequence ID" value="AAM01635"/>
        <property type="gene ID" value="MK0420"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0420"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.449"/>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="OMA" id="CGEYTLQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="SSF144210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000149019" description="Ribosome biogenesis protein Nop10">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="66" mass="7755" checksum="E451982914343427" modified="2002-06-01" version="1">MPKRLRRCKECGEYTLQRDKCPHCGGDLEVPHPHRFSPEDPYGKYRRKLKKRVWAEKFGPPSGEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY94</accession>
    <name>Q8TY94_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0411</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0411"/>
    <dbReference type="EnsemblBacteria" id="AAM01626">
        <property type="protein sequence ID" value="AAM01626"/>
        <property type="gene ID" value="MK0411"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0411"/>
    <dbReference type="HOGENOM" id="CLU_159086_1_0_2"/>
    <dbReference type="OMA" id="TKPCYGT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012031">
        <property type="entry name" value="UCP006577"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08979">
        <property type="entry name" value="DUF1894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006577">
        <property type="entry name" value="UCP006577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="97" mass="11159" checksum="6553CDACA7C070E9" modified="2002-06-01" version="1">MTFCLEEREYEILMARRPFDDCARYIESKFGNIVKLQPGEEILPGLRAIGYGKIPVAYGDEWIVLPITKPCHGSFVVKIEVSAEELEWFLKKHVSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA1</accession>
    <name>Q8TYA1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ca2+/Na+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ecm27_1</name>
        <name evidence="7" type="ordered locus">MK0404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01619">
        <property type="protein sequence ID" value="AAM01619"/>
        <property type="gene ID" value="MK0404"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0404"/>
    <dbReference type="HOGENOM" id="CLU_072251_0_0_2"/>
    <dbReference type="OMA" id="CEYFVNG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="4"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Na_Ca_ex" evidence="6">
        <location>
            <begin position="182"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="34133" checksum="1B17C12E02A1030C" modified="2002-06-01" version="1">MTLIAQIAASFGALLLGAWLFTNTVEWISYRFKLPSGFTGSFIAAVATALPETLVPIVAIIAGYREGVAVGAILGAPLMLSTVAMGIGGLSVLAAYLMGRRRRPVIKTSHFSLDARHFLVAYSLVLAVSLTDFKPAHFAVAAVLFLLYLVYVRRLLRTGDVVEQPSIELEMAHPVLAGLLAAVFLVGSVALLVAGAHGFADAVERLAERLGADPFTVSCLLAPIATELPEKLNSVIWYLKGRDDLALGNVTGAMVFQATFPVAVGLLFTSWRLGSRELATVTVPLAAMVLLYLYSRRNGLDWKVMSAVAVLYPVPFVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA3</accession>
    <name>Q8TYA3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted RNA-binding protein containing THUMP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0402"/>
    <dbReference type="EnsemblBacteria" id="AAM01617">
        <property type="protein sequence ID" value="AAM01617"/>
        <property type="gene ID" value="MK0402"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0402"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.429"/>
    <dbReference type="HOGENOM" id="CLU_795999_0_0_2"/>
    <dbReference type="OMA" id="HANYNER"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="51"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="333" mass="36439" checksum="483AF96F1F168255" modified="2002-06-01" version="1">MELLLTLAPDVRPEETERMARKIQDKLKLPAEPDPLKPVRRLRIEGVEDPDGIVSKLREEFPEVSRVVIVKRRGRSHDLDRIAAQAAKLARGEILPHHTFAVDARRLDKDLPYTSRDLAIKVGEAVRRVTGASVDLDSPDRYVDVHVSRHGHLIGITPATLREPHRSWLPSGAFKHVHVCCERPETEYEIADLIRITAALGLGSLILVEPNRDAVRGAEEKVGASSLIDLRIEEDLKEALAEFDVVVGLHPTAPNAESELLRAVEGANQICLLTGSETKGLSREAKELADVLVHLGPTTAEPMRTANAVAYAVGVLAARTVSLGSATAPTLHR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC2</accession>
    <name>RRP42_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exosome complex component Rrp42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rrp42</name>
        <name type="ordered locus">MK0380</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Contributes to the structuring of the Rrp41 active site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the archaeal exosome complex. Forms a hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. The hexameric ring associates with a trimer of Rrp4 and/or Csl4 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family. Rrp42 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC2"/>
    <dbReference type="SMR" id="Q8TYC2"/>
    <dbReference type="STRING" id="190192.MK0380"/>
    <dbReference type="EnsemblBacteria" id="AAM01595">
        <property type="protein sequence ID" value="AAM01595"/>
        <property type="gene ID" value="MK0380"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0380"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.406"/>
    <dbReference type="HOGENOM" id="CLU_038194_0_0_2"/>
    <dbReference type="OMA" id="PESVFEM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11365">
        <property type="entry name" value="RNase_PH_archRRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00622">
        <property type="entry name" value="Exosome_Rrp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020869">
        <property type="entry name" value="Rrp42_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0271">Exosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139998" description="Exosome complex component Rrp42">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00622"/>
        </source>
    </evidence>
    <sequence length="267" mass="29277" checksum="416CF6D5444E7A1E" modified="2002-06-01" version="1">MDLDLLARIKRHEVLAAIRAGERIDGRDFEEFRPIEVRAGVISKANGSALVRLGNTQLVVGVKLEVGRPYPDSPNEGALAVNAELVPLADPSFEPGPPDENAIELSRVVDRGIRESEMIDLEELCIEEGEHCWVTFVDIHVLDHDGNLFDASMIGSVSALSITEVPKAEVVDDEVEVMEEDTEPLAINDFPISVTIAKVGEYLLVDPCLEEEVIMDTRLTVTVTESGEVCAVQKGELGDFPEHLLEDAIDLATKKAEEVRRTVKAQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF0</accession>
    <name>Y350_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein MK0350</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0350</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYF0"/>
    <dbReference type="EnsemblBacteria" id="AAM01565">
        <property type="protein sequence ID" value="AAM01565"/>
        <property type="gene ID" value="MK0350"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0350"/>
    <dbReference type="HOGENOM" id="CLU_172276_3_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000053414" description="UPF0248 protein MK0350">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <sequence length="74" mass="8739" checksum="D033CA40786A1B96" modified="2002-06-01" version="1">MLHDPNDDPSEYVIYVIDRGSSSGLRRIWGDEIQDVKRGFLVLWNAEIPVHRVIRVERGGRVIWERGRRARGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG6</accession>
    <name>KHSE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine kinase</fullName>
            <shortName evidence="1">HK</shortName>
            <shortName evidence="1">HSK</shortName>
            <ecNumber evidence="1">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thrB</name>
        <name type="ordered locus">MK0333</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYG6"/>
    <dbReference type="SMR" id="Q8TYG6"/>
    <dbReference type="STRING" id="190192.MK0333"/>
    <dbReference type="EnsemblBacteria" id="AAM01548">
        <property type="protein sequence ID" value="AAM01548"/>
        <property type="gene ID" value="MK0333"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0333"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.354"/>
    <dbReference type="HOGENOM" id="CLU_041243_1_1_2"/>
    <dbReference type="OMA" id="CANRIPH"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156642" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <sequence length="298" mass="32193" checksum="85F2D6E937D839FE" modified="2002-06-01" version="1">MTPLSTVVRAPATIANVGPGFDVFGLAVDGFHDVVEAHEADGVRIVTEDPIPTDPERNTAGRVALRMVEEFDLPGVSLEIRKGVPMGGLGSSAASAVAAAVAIDREFELGLEESELLRFAAEGERAAAGEPHYDNVAPCLLGGFVIWRFEREYVRLEVPGDLRFVTVTPTGVRVTTEEARKALRERPPSLDDVVNNLSAVALMVHALHEEDAETFARMVGWDRISEPVRKRFVPRYRELRETAYGTGALGFAISGAGPTVFAVCWREDAEDVRTALEDVLDGKCVSAVHRVSDGAEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG8</accession>
    <name>Q8TYG8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01546">
        <property type="protein sequence ID" value="AAM01546"/>
        <property type="gene ID" value="MK0331"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0331"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="23877" checksum="BD1292529A168F1E" modified="2002-06-01" version="1">MVGSHVPIPHDPVERIRALRVLREVYRRGKKPSLEVTYRTVNGSTCGPYYVARWRRDSRHKRGRTLYLGKPENKSVRFVEWLVSLDREEVLELARQLMRNLRSVLKTLLTEVSDLPYKKARRILARGLALAFDARPAGSPGIRDVLEKLPDRLESFLIRTLGGWPAHYSSYLGKVIHSRRKSLDGKHEIPDVKLELERWKLRHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI8</accession>
    <name>Q8TYI8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase involved in chromosome partitioning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01526">
        <property type="protein sequence ID" value="AAM01526"/>
        <property type="gene ID" value="MK0309"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0309"/>
    <dbReference type="HOGENOM" id="CLU_864987_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="321" mass="35685" checksum="47C4974C4467F858" modified="2002-06-01" version="1">MPRARGGCGLPSREPAAPFLVGFTAYKGGTGKSTVAFNVGLEFARYYQGFDGVPRRPVVFFDLDPQGRSDEGGRTLKKMFEDGPFRLRSLHSPIPELDVQVVEYDPDEPVPVDGVNGSFGSYGLVDSDASFAPALLAVPKNIRIRRVKPLRRLLAAANELLFQYLEDRGSLDSKLREYLSSPPLGVVDTPPMNAASDTFAGILTQVDLVIPVVDPENVRQLSLFCKLYVTRLAVVNRVPPDGLTREAKFFDGVLNRILNKIPLNKDEVVKIPERPVIRFASALGIPVRARPDKRHSGYFREVARHIRADWNRRRYGSGVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK2</accession>
    <name>Q8TYK2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0294</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01511">
        <property type="protein sequence ID" value="AAM01511"/>
        <property type="gene ID" value="MK0294"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0294"/>
    <dbReference type="HOGENOM" id="CLU_036781_1_1_2"/>
    <dbReference type="OMA" id="GTMLDHV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025105">
        <property type="entry name" value="DUF4010"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003416">
        <property type="entry name" value="MgtC/SapB/SrpB/YhiD_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13194">
        <property type="entry name" value="DUF4010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02308">
        <property type="entry name" value="MgtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4010" evidence="7">
        <location>
            <begin position="181"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="420" mass="44199" checksum="2383DAB76C4C6DB3" modified="2002-06-01" version="1">MDEYLRFLQMLVLSAAVGMVVGLEREHAHIARRIAGFRTFTLIGVLGGLTAYFYELGCVPIATVTSVGAAALAVTMYVMRVYQRRLLGIVTPLALLATFAAGALVGMERFKEGATLAIATAVVLLSRARIHPVLRRLSDRDVLDALTVFSLAALVYPLCPPGPVDPWGVLNLRRVLEIVLLVLAVTALGYLGVRMSVRGGVLTTSLVGGFVSSSATTATVVARFRELPWVPAAVVPAATSVAIIRNMILVAAVSRNLAVVKKVACVAIPAAIAGFAVAVYESGRAEVDEEDLRRVVRGRMRSPLSLKPAVKLGLLISGFSMTLKIGQLFAGKAGLVVGAVVGSLASSNAMSLSLAYMVNSGTLSSSTAGILIALSSLESILVKYLWTAVFGKREVLERTWRTLLPPTIAGIAGFGVVLAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM3</accession>
    <name>Y273_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0235 protein MK0273</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0273</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYM3"/>
    <dbReference type="SMR" id="Q8TYM3"/>
    <dbReference type="STRING" id="190192.MK0273"/>
    <dbReference type="EnsemblBacteria" id="AAM01490">
        <property type="protein sequence ID" value="AAM01490"/>
        <property type="gene ID" value="MK0273"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0273"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.276"/>
    <dbReference type="HOGENOM" id="CLU_130694_6_1_2"/>
    <dbReference type="OMA" id="IGKINEW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005228">
        <property type="entry name" value="CHP00251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="SSF69786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00251">
        <property type="entry name" value="TIGR00251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139469" description="UPF0235 protein MK0273">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <sequence length="96" mass="10764" checksum="6A95F6394180B76C" modified="2002-06-01" version="1">MESPVKEHREGTLIRVRVNPDADTTDLKGVDEWRGVLEVDVAAPPVKGKANRELLEFLGRKLNTTCELVSGEKSREKLVLARDVSVDEVKERLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN2</accession>
    <name>Q8TYN2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0263"/>
    <dbReference type="EnsemblBacteria" id="AAM01480">
        <property type="protein sequence ID" value="AAM01480"/>
        <property type="gene ID" value="MK0263"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0263"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.266"/>
    <dbReference type="HOGENOM" id="CLU_117547_0_0_2"/>
    <dbReference type="OMA" id="GCPDVPY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021778">
        <property type="entry name" value="Se/S_carrier-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11823">
        <property type="entry name" value="DUF3343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3343" evidence="1">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="20989" checksum="233666424FEEA23D" modified="2002-06-01" version="1">MEMGLLSRLRSAFSRDEGPSDEREGKGLIVFESARDAMKAERTLKEAGYDVRAVAPPPEIREGCDLAIEYDLVDEVGVRRTLEEIGVEPLKFVSLEDPSLKPIELVRVKEVDGYIMVRCGNMKVTVDRDGTIVNVSGGGCPDVPYLAHELVGKNVLELSENSMPASLGYTLCAYTLDKAVRKAKEVLLGEGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN6</accession>
    <name>Q8TYN6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted redox protein, regulator of disulfide bond formation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0257"/>
    <dbReference type="EnsemblBacteria" id="AAM01474">
        <property type="protein sequence ID" value="AAM01474"/>
        <property type="gene ID" value="MK0257"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0257"/>
    <dbReference type="HOGENOM" id="CLU_165255_0_0_2"/>
    <dbReference type="OMA" id="VVGDYKP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="77" mass="8921" checksum="5D581191F0D382BB" modified="2002-06-01" version="1">MVEYDEELDVRGKICPMPVLETRKKLEEMSEGEVLKVVGDYPPAKDNIRRFAEENGHEVLDVEEGEDHFVIYIKKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP3</accession>
    <name>Q8TYP3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01467">
        <property type="protein sequence ID" value="AAM01467"/>
        <property type="gene ID" value="MK0250"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0250"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.253"/>
    <dbReference type="HOGENOM" id="CLU_1113873_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="26187" checksum="04AAABC18B53641D" modified="2002-06-01" version="1">MRASAIATLLVAMVVIPIASAAELQTSATTETAPTATVKPSAGADAGSDVGVSVVGVPLTIGTDETKSTSTTGSANVTTTIPPEYYSLASGLTQSLCDALSNGVAAVIDCLRGQAYHEPRPEDIVFAVVLSAVKAVVNQKLHEESFQKPLNEFHLMLVQFIGPNATLMQLLSAVTAELQVNLKEAIKSFGPIAMLYDRVENALKSASGNEKSAYESAKSELDNMVNNLTKYASDIFCMLLSKCFPGLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU1</accession>
    <name>Q8TYU1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01418">
        <property type="protein sequence ID" value="AAM01418"/>
        <property type="gene ID" value="MK0201"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0201"/>
    <dbReference type="HOGENOM" id="CLU_2550278_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="82" mass="9122" checksum="13E6BA2A2B2C9320" modified="2002-06-01" version="1">MSKSEAMAHLSVMEATYNVKIKNKEEAAEAIAEKAMEFGVEPIHICTALNTWLARMMSEGEIEEGDEIEIPRELIEGIEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV0</accession>
    <name>Q8TYV0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01408">
        <property type="protein sequence ID" value="AAM01408"/>
        <property type="gene ID" value="MK0191"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0191"/>
    <dbReference type="HOGENOM" id="CLU_1076103_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="258" mass="28057" checksum="6B800561927C7501" modified="2002-06-01" version="1">MKLTILASPLADRDVLRRAERLADRFNLEPRIVTDPEDVPDSPTVALVATGGTEDTIEALLERSSAVLLLHWHSYNSLAAALEVGVSTLHVGRAEEAIRAFKRLVGTRILVVGGKCSWIRGPDPSDLPYLQTVEVDLEDVKSVDPDGSFEEKLRAHGIDPSELNRNFLDLAGKLHAVLSQYGANAITGDCFSLYEEFGAGSLSVLLRAGRALLLRGRSRRPGHDPANARAYPSLHRKRDRAGSLRDASRPLCLPVGVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY7</accession>
    <name>TRM1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trm1</name>
        <name type="ordered locus">MK0154</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYY7"/>
    <dbReference type="SMR" id="Q8TYY7"/>
    <dbReference type="STRING" id="190192.MK0154"/>
    <dbReference type="EnsemblBacteria" id="AAM01371">
        <property type="protein sequence ID" value="AAM01371"/>
        <property type="gene ID" value="MK0154"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0154"/>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="OMA" id="VFYNPVM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="PTHR10631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000197035" description="tRNA (guanine(26)-N(2))-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Trm1 methyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <sequence length="383" mass="43035" checksum="B12630AD3A0784D5" modified="2002-06-01" version="1">MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGVGARGIRIAVELSPEVVVLNDLNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAGRFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSALAGRYPRSARRKYWVEVERVEFYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYLLHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAELLETVAKETGTNPWAYDIHRWASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKTDASPEEFEAVLTEVAGDSGCLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ20</accession>
    <name>Q8TZ20_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">serB</name>
        <name evidence="12" type="ordered locus">MK0121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0121"/>
    <dbReference type="EnsemblBacteria" id="AAM01338">
        <property type="protein sequence ID" value="AAM01338"/>
        <property type="gene ID" value="MK0121"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0121"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.120"/>
    <dbReference type="HOGENOM" id="CLU_036368_4_3_2"/>
    <dbReference type="OMA" id="CINEPDL"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM01338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="217" mass="23725" checksum="540D294F5B2660BB" modified="2002-06-01" version="1">MAQARRLVVLDFDGTLVDGETIDLIAEAAGVDDEVEEITRRAMRGELEFGEALRERVRLLAGTPASVLDEVVTELRLNPGVREFVAAVRSVGAAVAVISGGFTEVVSHFCRELGLDAYVANELEVRNGFLTGRVYGPVMSSSAKGRVLMELCRRFGTRPEDTVAVGDGANDASMLKRVGLPLGFRPKKPLYEIIEMAFDDFRRAVPVVLRFWGVPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ35</accession>
    <name>Q8TZ35_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-40 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01321">
        <property type="protein sequence ID" value="AAM01321"/>
        <property type="gene ID" value="MK0104"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0104"/>
    <dbReference type="HOGENOM" id="CLU_310077_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="886"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="934"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="949" mass="105696" checksum="7FA48C41C19C11CD" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ36</accession>
    <name>Q8TZ36_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative asparagine synthetase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="3">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">asnB</name>
        <name evidence="6" type="ordered locus">MK0103</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0103"/>
    <dbReference type="EnsemblBacteria" id="AAM01320">
        <property type="protein sequence ID" value="AAM01320"/>
        <property type="gene ID" value="MK0103"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0103"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.103"/>
    <dbReference type="HOGENOM" id="CLU_014658_4_0_2"/>
    <dbReference type="OMA" id="DWSGIYS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="4">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="520" mass="57806" checksum="5ECAB731D34F4165" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ43</accession>
    <name>Q8TZ43_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01313">
        <property type="protein sequence ID" value="AAM01313"/>
        <property type="gene ID" value="MK0096"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0096"/>
    <dbReference type="HOGENOM" id="CLU_2010112_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC" evidence="2">
        <location>
            <begin position="16"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="123" mass="13495" checksum="70BB1762759595F4" modified="2002-06-01" version="1">MTNGESSNEEEYRKRYVRFQRLLGMEVFTEDGRRVGTVEDVTFDPRTGDLVRFLVIVTEQPSGGGLLPLPGGGGRRTETVDAELVKAVGDIIIIESPEKASSGEEGKRKKQESSPAKPSDLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ57</accession>
    <name>Q8TZ57_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate:ferredoxin oxidoreductase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">porA_1</name>
        <name evidence="3" type="ordered locus">MK0082</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0082"/>
    <dbReference type="EnsemblBacteria" id="AAM01299">
        <property type="protein sequence ID" value="AAM01299"/>
        <property type="gene ID" value="MK0082"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0082"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.83"/>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="OMA" id="WNDQQDS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="16"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="264"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="383" mass="42207" checksum="67B5FD0706D38FD4" modified="2002-06-01" version="1">MTEVHVINGNYAVAEAVRMVDVDVIAAYPITPQTPIVEYLSEFVSNGELDAEFIHVESEHSAISAVLGASATGARVFTATASQGLALMHEILFIASGLRLPIVMAVANRALSAPINIWCDHSDSVAQRDTSWIQLYCESNQEVFDTVVQAYRIAEHEDVLLPVMVCLDGFTLSHTLEPVELPEEEEVRSFVGKYEPTHCYLDPEDPMTLGPVGGPDSYMEFKKMQHDAMEKAREVIGEVNREFSDEFGRSYGDGLIEEYNTEDADYVVIAMGSVCGTVKHVIDEERPDVGLVRVKAYRPFPGDRIVEVIQDKEGVVTIDRAHSYGAMPPLWTDVKAHAPDVDVSSTIAGLGGRDIRPQDVLEIIKVAEEGKGMDEPVWINVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ70</accession>
    <name>Q8TZ70_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01286">
        <property type="protein sequence ID" value="AAM01286"/>
        <property type="gene ID" value="MK0069"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0069"/>
    <dbReference type="HOGENOM" id="CLU_2420053_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="91" mass="10507" checksum="140A9AC622B18AD2" modified="2002-06-01" version="1">MSRIGEIVTEMFQDGRLFLGMGEYAQTVAYDELLLWFDVLFDDEHILDEGDRVERKVGKLLTALYYGTVGYTVTEHTDEGNNSWIYVHAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ73</accession>
    <name>Q8TZ73_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Biopolymer transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tolQ</name>
        <name evidence="9" type="ordered locus">MK0066</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01283">
        <property type="protein sequence ID" value="AAM01283"/>
        <property type="gene ID" value="MK0066"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0066"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.67"/>
    <dbReference type="HOGENOM" id="CLU_088835_1_0_2"/>
    <dbReference type="OMA" id="FIHIVSE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="83"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="202" mass="22619" checksum="4D4DC96FA0C5373B" modified="2002-06-01" version="1">MVSIATAITQALYVVMELLFYPTIVLLLLFLAYSLMELAIFLREVAYRPRFTRKDLEQIADKFPDVKRLPPHFSEFLRRVEKIVEKSDSADEASLKVEKELENLENFLASKVSRTRIVVRLGPMLGLMGTLIPLGPGLEQLAKGNIAGLATSLITAFATTVVGLASAGACYVITLFRSRWYRKDVSDLEFLAETILQREFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ78</accession>
    <name>Q8TZ78_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Single-stranded DNA-specific exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">recJ</name>
        <name evidence="3" type="ordered locus">MK0061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0061"/>
    <dbReference type="EnsemblBacteria" id="AAM01278">
        <property type="protein sequence ID" value="AAM01278"/>
        <property type="gene ID" value="MK0061"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0061"/>
    <dbReference type="HOGENOM" id="CLU_071985_0_0_2"/>
    <dbReference type="OMA" id="ETVIFTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="1">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="215"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="340" mass="37580" checksum="D12C1079E63CEC62" modified="2002-06-01" version="1">MDPTKEVRNALVPHLKKLKDERVAVISDIDVDGLASAAILGHVLNNVGADFVLYFSPPANFEEVVSGIAPECDVLIAADLGSTGETAIHDAKSEGCRVIVVDHHQVLEDVRPDVFIHDTRLCAAELCYWATVDLHERDLRLIAAYAAFTDYAEEYSRVLRETLKMYDRRKVYTEASLLDYALIRMDGEDRRELALKLPETPVSELDEVYELARDAMAEEHEVLRRAREVAECLNEVVAYAIMDDVHPAMTGRVASHVAGVKRRPVGVCVRRGKISARVVEGEEIDVARAIRKAATEVGGKGGGHAPAAGGVVPESRVEEFLRLFGEEVKRQVESARTRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ85</accession>
    <name>Q8TZ85_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitrogen regulatory protein PII</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0054"/>
    <dbReference type="EnsemblBacteria" id="AAM01271">
        <property type="protein sequence ID" value="AAM01271"/>
        <property type="gene ID" value="MK0054"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0054"/>
    <dbReference type="HOGENOM" id="CLU_082268_0_1_2"/>
    <dbReference type="OMA" id="YRGTEHV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="117" mass="13207" checksum="A528694AF1CC1513" modified="2002-06-01" version="1">MYMIEAVIRPEKLDDVKEALDEAGYPGMTVIHVKGRGRQRGIVHRYRDEEYRTDLLDKILLKVAVPTEDDVEKVIDVICEHAKTGRPGDGMIFVIPLEDAVRARTGERGDDALSTEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC1</accession>
    <name>Y012_METKA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0254 protein MK0012</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0012</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0254 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZC1"/>
    <dbReference type="SMR" id="Q8TZC1"/>
    <dbReference type="STRING" id="190192.MK0012"/>
    <dbReference type="EnsemblBacteria" id="AAM01229">
        <property type="protein sequence ID" value="AAM01229"/>
        <property type="gene ID" value="MK0012"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0012"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.11"/>
    <dbReference type="HOGENOM" id="CLU_1451416_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00673">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009625">
        <property type="entry name" value="HcgF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06787">
        <property type="entry name" value="HcgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018786">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147368" description="UPF0254 protein MK0012">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="182" mass="19891" checksum="94E06A1C3E8DC9D5" modified="2002-06-01" version="1">MLRVATAECFTHGFVGREIHASASGYTGELGSEILGTELEGKVSVVAACFIPTVSGLRSLLGIDPPEPDEVSKSGAKAYREETDRKVAVMMARAVRERTGADVGIGTTAGIGRGAICLDDGEITLLGRTDVHANLLKPDERIRKRQLQGIKRSLVMFRAYFRCELDELLEEEWVEEVTRDLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1993-07-01" modified="2022-05-25" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q02394</accession>
    <name>HMD_METKA</name>
    <protein>
        <recommendedName>
            <fullName>5,10-methenyltetrahydromethanopterin hydrogenase</fullName>
            <ecNumber evidence="1">1.12.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>H(2)-dependent methylene-H(4)MPT dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">N(5),N(10)-methenyltetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hmd</name>
        <name type="ordered locus">MK0013</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1992" name="Eur. J. Biochem." volume="208" first="511" last="520">
            <title>H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea.</title>
            <authorList>
                <person name="Zirngibl C."/>
                <person name="van Dongen W."/>
                <person name="Schwoerer B."/>
                <person name="von Buenau R."/>
                <person name="Richter M."/>
                <person name="Klein A."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="1521540"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1992.tb17215.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Eur. J. Biochem." volume="245" first="386" last="391">
            <title>Overexpression of the coenzyme-F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase gene from the hyperthermophilic Methanopyrus kandleri.</title>
            <authorList>
                <person name="Klein A.R."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9151968"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.1997.t01-1-00386.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1991" name="Arch. Microbiol." volume="156" first="43" last="48">
            <title>N5, N10-methylenetetrahydromethanopterin dehydrogenase (H2-forming) from the extreme thermophile Methanopyrus kandleri.</title>
            <authorList>
                <person name="Ma K."/>
                <person name="Zirngibl C."/>
                <person name="Linder D."/>
                <person name="Stetter K.O."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="1772345"/>
            <dbReference type="DOI" id="10.1007/BF00418186"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-24</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="1.12.98.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:20018"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:20019"/>
        </physiologicalReaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Activity requires salt; 100 mM potassium phosphate, potassium chloride, and sodium chloride are equally effective.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">2 uM for H(+)</KM>
            <KM evidence="1">50 uM for 5,10-methylenetetrahydromethanopterin</KM>
        </kinetics>
        <temperatureDependence>
            <text evidence="1">Thermolabile at 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (hydrogen route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2" evidence="1"/>
    <dbReference type="EMBL" id="X60719">
        <property type="protein sequence ID" value="CAA43127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="Y10251">
        <property type="protein sequence ID" value="CAA71299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="S30603">
        <property type="entry name" value="S30603"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q02394"/>
    <dbReference type="SMR" id="Q02394"/>
    <dbReference type="STRING" id="190192.MK0013"/>
    <dbReference type="EnsemblBacteria" id="AAM01230">
        <property type="protein sequence ID" value="AAM01230"/>
        <property type="gene ID" value="MK0013"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0013"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.12"/>
    <dbReference type="HOGENOM" id="CLU_772960_0_0_2"/>
    <dbReference type="OMA" id="HACTIPT"/>
    <dbReference type="BRENDA" id="1.12.98.2">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00696"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01090">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010062">
        <property type="entry name" value="HMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500165">
        <property type="entry name" value="HMDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01723">
        <property type="entry name" value="hmd_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000218509" description="5,10-methenyltetrahydromethanopterin hydrogenase">
        <location>
            <begin position="2"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="1772345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="1521540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="1772345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="358" mass="39038" checksum="9B8491B2A5A20771" modified="2007-01-23" version="3">MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGVDEVVLSDPVFDADGFTVVDIEEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPKPPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWLPKGGVQPDIFKKIIDDIPEGCIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYELGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEALQMMAKFMEEEGLDKLEEALDPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1MVQ4</accession>
    <name>Q1MVQ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwdD</name>
        <name evidence="2" type="ordered locus">MK1526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1526"/>
    <dbReference type="EnsemblBacteria" id="AAM02739">
        <property type="protein sequence ID" value="AAM02739"/>
        <property type="gene ID" value="MK1526"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1526"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1686"/>
    <dbReference type="HOGENOM" id="CLU_123704_1_0_2"/>
    <dbReference type="OMA" id="GMIYIPM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012040">
        <property type="entry name" value="Formylmethanofuran_DH_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015873">
        <property type="entry name" value="FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molydop_binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="131" mass="14462" checksum="51E291A46B706FEC" modified="2011-05-03" version="1">MAEFTLLTGRTIWQGEAIETDKAGEIYPNATAICYFNPKDMSELGISEGDPVKVKSKYGEVIVRAKAADTDVPPRGIVFIPMGPWANKVVDPNTKSTGMPGFKGIKVEIEPTDEEPIEDMSEFMRSEYLSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q49611</accession>
    <name>FWDG_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G</fullName>
            <ecNumber evidence="2">1.2.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase II subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fwdG</name>
        <name type="synonym">fudG</name>
        <name type="ordered locus">MK1525</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Mol. Microbiol." volume="23" first="1033" last="1042">
            <title>A selenium-dependent and a selenium-independent formylmethanofuran dehydrogenase and their transcriptional regulation in the hyperthermophilic Methanopyrus kandleri.</title>
            <authorList>
                <person name="Vorholt J.A."/>
                <person name="Vaupel M."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9076739"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.1997.2931653.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile. May function as an electron transfer protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Not inactivated by cyanide.</text>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="induction">
        <text>By growth on tungsten or molybdenum under anaerobic conditions.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="2"/>
    <dbReference type="EMBL" id="X98917">
        <property type="protein sequence ID" value="CAA67417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q49611"/>
    <dbReference type="SMR" id="Q49611"/>
    <dbReference type="STRING" id="190192.MK1525"/>
    <dbReference type="EnsemblBacteria" id="AAM02738">
        <property type="protein sequence ID" value="AAM02738"/>
        <property type="gene ID" value="MK1525"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1525"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1685"/>
    <dbReference type="HOGENOM" id="CLU_139698_5_4_2"/>
    <dbReference type="OMA" id="WGGKGPY"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159254" description="Tungsten-containing formylmethanofuran dehydrogenase 2 subunit G">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="3">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="3">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q48943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="79" mass="8345" checksum="0EFD1B298A8BBA67" modified="1996-11-01" version="1">MVKIVIHEERCHGCGNCVIACPVNACNSPNVWGGKGPEDGEDVVIKVVNGTVSVINEDLCEACMTCELACPVDAIEIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ3</accession>
    <name>Q8TGZ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0673</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0673"/>
    <dbReference type="EnsemblBacteria" id="AAM01888">
        <property type="protein sequence ID" value="AAM01888"/>
        <property type="gene ID" value="MK0673"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0673"/>
    <dbReference type="HOGENOM" id="CLU_005316_7_3_2"/>
    <dbReference type="OMA" id="RIRNTRM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="5">
        <location>
            <begin position="23"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="307" mass="34314" checksum="BFD72F6BEB1A63BE" modified="2002-06-01" version="1">MRYVEEFRERLERIFGDRAEDVYRYLLEGRPPTYVRVNTLKADVEEVVENLETAGVELAETPLSYAFRVLSSPGPLGSSLEHVAGYLYLQDLASMVPPELLDPEPPGPVIDLCAAPGSKTTQLAQLLGGEGVVLAVDADPRRVRALVHNVNRLGCVNVIVAHADAARLRISAPFLLLDPPCSGEGTLHRDPHALRTWTPKKPGRFARTQLRLLRAALRMLPPGGRLVYSTCTFSVEENELVIHEALGNDDRYRIVPSVPRWLEPHTVPGLTEWEGRELREDLRHAFRIDPASLESDGFFVAVIERRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV9</accession>
    <name>Q8TUV9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02857">
        <property type="protein sequence ID" value="AAM02857"/>
        <property type="gene ID" value="MK1644"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1644"/>
    <dbReference type="HOGENOM" id="CLU_3039057_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="54" mass="5618" checksum="5DEF800C61C81098" modified="2002-06-01" version="1">MEERDEGDYVVVKCRECGHVIVRRKVGSAGLTTETLGIAVAAAVTATAIASTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX1</accession>
    <name>Q8TUX1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1632"/>
    <dbReference type="EnsemblBacteria" id="AAM02845">
        <property type="protein sequence ID" value="AAM02845"/>
        <property type="gene ID" value="MK1632"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1632"/>
    <dbReference type="HOGENOM" id="CLU_2243866_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="SSF158372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="104" mass="11806" checksum="AEFC6F14DB99B3D0" modified="2002-06-01" version="1">MPRGRDHEPGRVARGEGNHREVAAHLGEKIEKYISKLPSVLNECKPRDRAGERLLDLASRYFYDARYFLDRGEMVEAFTCLSYAWALLRAGAEVGVLDVPEDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX5</accession>
    <name>Q8TUX5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="14">BCAT</shortName>
            <ecNumber evidence="14">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14 15" type="primary">ilvE</name>
        <name evidence="15" type="ordered locus">MK1627</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 14">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 14">
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14">
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6 14">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 14">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 14">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9 13">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="14"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1627"/>
    <dbReference type="EnsemblBacteria" id="AAM02840">
        <property type="protein sequence ID" value="AAM02840"/>
        <property type="gene ID" value="MK1627"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1627"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1790"/>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_2"/>
    <dbReference type="OMA" id="GMQCYEG"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 15" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="12 14" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 15" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AAM02840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="295" mass="32956" checksum="5DB962E995D549BB" modified="2002-06-01" version="1">MSEREQLIYLNGELVPREEAKISVYDHGFLYGDGVFEGIRAYDGRIFKLDEHVDRLYDSAKAIMLEIPMTKEKMKEAIIETVRANELRDAYIRVVVSRGEGDLGLDPEKCPEPNVVIIAEPMEPLYGDLYEKGIEVITASVRRIPPDALDPKIKSCNYLNNILAKIQANLAGADEAIMLDHEGYVCEGTGDNVFVVEDGTVYTPPEDTILRGITRATVMEICEELGIPVEEKRITLGELYAADEVFLTGTAAEVAPVRKVDGRKIGEECPGPITRRIMEAFRELTKKEGTPVYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV25</accession>
    <name>Q8TV25_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK1576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1576"/>
    <dbReference type="EnsemblBacteria" id="AAM02789">
        <property type="protein sequence ID" value="AAM02789"/>
        <property type="gene ID" value="MK1576"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1576"/>
    <dbReference type="HOGENOM" id="CLU_544867_0_0_2"/>
    <dbReference type="OMA" id="WQLTMIS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="491" mass="52945" checksum="0A087E1EE2981B9B" modified="2002-06-01" version="1">MEKVGLRRELTMLGFISTICCTVIGGGVNVLTCMIQVKAPGVGPYVPLAFLIGAVPSLVAGIVAGALSAAVPRAGGHYTYVSRLFDPFLAFFSSWSRFVGEIGAFVAIAIGDVALLAAMCKFWGAPAAGKWLNAHTLEVATLIIIFTWLVNVLGIRIYEAVVDVMFFILLTGFFIVVGYGFAHSPAEYLKAIGGTTALKSLISQAGGLPKDVGTLSAIFSAAATLVFAYVGFETGTQAAGEVKKPEKTVFRGMLIALGIITAYYLLYSAAVYHAVPWEYIYAKAALADKAGSNFTVPEAMAPIMPSALAGYVAFVAAIALLSDLPPMFLSTSRMTFAWAYDGMFPKVFAKVHKSFGTPHWALTVLMVVSVILTWAVGKFLAAVDITTVALLFTYLFICMTGLVWKFVRPDIYEKAPLGDKTKKAVNWLGATGTVLSLFFLGEIAISDPTSFYWWIALMVPGPFIFYYAYNRTVKKLGPEKARERLMTIPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV31</accession>
    <name>Q8TV31_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02782">
        <property type="protein sequence ID" value="AAM02782"/>
        <property type="gene ID" value="MK1569"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1569"/>
    <dbReference type="HOGENOM" id="CLU_827977_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="335" mass="34322" checksum="E3524D2B50E82AB9" modified="2002-06-01" version="1">MSVVHWSLYVNSAVISAAAALVLPNVPVYVYRHGGDEFTASLVLSTFSITMTITSLASTSLLEGVRAGLPICLGCLGYGWALLPFFVHDSVASLAFARALQGVCSGFSFPANVAAALQLGRSAVSKNNFWSNVGFIVGYLASSHVRDPFGICSVLSALAAVFGLPLLTLRIQSQEDVSAYHGYPVAFGAALALTLASTLPNSAITLLAPKYGGDYGLVIAMMTAVGGVAQLLGPRLGELESTLLAVVLCVVSAALGYSLASVLAAGTATGLMYYAANVYSGGGRGVRAVSTAIGVGYAVNQTIVGAILSAGLDGWRFATLMAAAITLVSLLGVRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV34</accession>
    <name>DUT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="1">dUTPase</shortName>
            <ecNumber evidence="1">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dut</name>
        <name type="ordered locus">MK1566</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV34"/>
    <dbReference type="SMR" id="Q8TV34"/>
    <dbReference type="STRING" id="190192.MK1566"/>
    <dbReference type="EnsemblBacteria" id="AAM02779">
        <property type="protein sequence ID" value="AAM02779"/>
        <property type="gene ID" value="MK1566"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1566"/>
    <dbReference type="HOGENOM" id="CLU_103451_2_0_2"/>
    <dbReference type="OMA" id="WDAGYEG"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00635">
        <property type="entry name" value="dUTPase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023537">
        <property type="entry name" value="dUTPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153639" description="Probable deoxyuridine 5'-triphosphate nucleotidohydrolase">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00635"/>
        </source>
    </evidence>
    <sequence length="154" mass="17315" checksum="203B7FED196C8958" modified="2002-06-01" version="1">MILGEYVLRDLFPDLDEDQYQPAGIDLKLDRVFRLEGRGALLEGDDKRLPEYREVETEGGVFELEPNVPYVLELAPELEIPEDTAVLFLPRSTLLRSGVSVHTALGDPGFRGKIRVLAVNHHAAPYRIAHGERVVQAVFLRAEDAGRYEGDYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV35</accession>
    <name>Q8TV35_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1565</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02778">
        <property type="protein sequence ID" value="AAM02778"/>
        <property type="gene ID" value="MK1565"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1565"/>
    <dbReference type="HOGENOM" id="CLU_1227660_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="225" mass="25415" checksum="44C5D7DA43C508A9" modified="2002-06-01" version="1">MLALFPLAHPFTITSEDLRSVWRWIKTPDVIRVYTDSESLAELLKRPLLRAVRSSLRPPTVSWTGPCPEEDLRRAGAEVEMDEHRPFETSDVLALCTRTFALFLKGVRGGAVGLVGFGDLDDLHRYLVELGYPRTFKATSTLMLRRREVEMDELWKLFPAWAGDDVEPLLRETLKPDVVEDLLEEARSLTSSLGPRVRDLESTPTGRLLLASSQTVWVVKGALNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV62</accession>
    <name>Q8TV62_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">p-loop ATPase of the PilT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK1536</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1536"/>
    <dbReference type="EnsemblBacteria" id="AAM02749">
        <property type="protein sequence ID" value="AAM02749"/>
        <property type="gene ID" value="MK1536"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1536"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1697"/>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="OMA" id="FADMRMA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="4">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="AAA" evidence="3">
        <location>
            <begin position="276"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="2">
        <location>
            <begin position="501"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="644" mass="71908" checksum="4A8C46411587C705" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV97</accession>
    <name>Q8TV97_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cysG_1</name>
        <name evidence="5" type="ordered locus">MK1495</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1495"/>
    <dbReference type="EnsemblBacteria" id="AAM02708">
        <property type="protein sequence ID" value="AAM02708"/>
        <property type="gene ID" value="MK1495"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1495"/>
    <dbReference type="HOGENOM" id="CLU_1574967_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="169" mass="18954" checksum="784AA20709682BC0" modified="2002-06-01" version="1">MPYVPLFVRVERAVIVGGGRVAERKARTLVDLGVDVIVVAPEESEWIRDLPVRFVRRKVKGPEDLPEADLYVVATDDPDLNARLERALSLVNRVDTPRPKVRFPSVLRSGDAVLAISTGKPRVTKALRMIAAELLGPTLRKAAELSEDARKWSLERVINELEGLRWQDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV98</accession>
    <name>Q8TV98_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-20 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1494</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02707">
        <property type="protein sequence ID" value="AAM02707"/>
        <property type="gene ID" value="MK1494"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1494"/>
    <dbReference type="HOGENOM" id="CLU_2629743_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="77" mass="8860" checksum="BFC579CB02ABF306" modified="2002-06-01" version="1">MSGAELIRELIMELRALRHEIHMLRVELEKLRSEVNEVEPDDDFSELLPQSEHVPEVVIEDSGLGPDSVAGERWRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB2</accession>
    <name>PYRB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">MK1480</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB2"/>
    <dbReference type="SMR" id="Q8TVB2"/>
    <dbReference type="STRING" id="190192.MK1480"/>
    <dbReference type="EnsemblBacteria" id="AAM02693">
        <property type="protein sequence ID" value="AAM02693"/>
        <property type="gene ID" value="MK1480"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1480"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1637"/>
    <dbReference type="HOGENOM" id="CLU_043846_1_2_2"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113248" description="Aspartate carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="309" mass="34690" checksum="32FDDA9B1E0F9974" modified="2002-06-01" version="1">MSSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRLSFESAMHRLGGDVISLGGKEAASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAELTDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGLLGDLKYGRTVHSLAYALALFGAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEEFERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMALLDLILGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC8</accession>
    <name>Q8TVC8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted nucleotidyltransferase of the DNA polymerase beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1464"/>
    <dbReference type="EnsemblBacteria" id="AAM02677">
        <property type="protein sequence ID" value="AAM02677"/>
        <property type="gene ID" value="MK1464"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1464"/>
    <dbReference type="HOGENOM" id="CLU_086367_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="226" mass="25884" checksum="95FB13870B90F91D" modified="2002-06-01" version="1">MALIQKRLNRRELEWVRKRVAREAARLLYEGAVDEYIDAKRLAARRLGVDVMPSNREVAIELDRIGDELEGEEKLHRLKRLREEALKVMEAIEDLEPRLIGSVWRGNIKRESDIDIAVLGTTDPEEVIERLKEAGIEVLDVEEVVITERGGKPLDIPEHYVNIKVRTPGGEKAEIGVTVSSPELERKGRVDLGRCDFFGDKITGLSVEELRKLLREDPYRKFIPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI8</accession>
    <name>Q8TVI8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1401</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02614">
        <property type="protein sequence ID" value="AAM02614"/>
        <property type="gene ID" value="MK1401"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1401"/>
    <dbReference type="HOGENOM" id="CLU_1965592_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014958">
        <property type="entry name" value="DGC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08859">
        <property type="entry name" value="DGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="121" mass="13228" checksum="B2C9B7D81A4C1F9C" modified="2002-06-01" version="1">MSRKALMPCTGMSPNGFVSRVVVAELSEEEGIPSICPPATAAGKEKFLELLKRVEVLAVAGCDYNCPARILREKAGKEPSETVFVSDVSEETGVDADSLYELTEANRELVKEVKRRVKELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI9</accession>
    <name>Q8TVI9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="4">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pncB</name>
        <name evidence="13" type="ordered locus">MK1400</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1400"/>
    <dbReference type="EnsemblBacteria" id="AAM02613">
        <property type="protein sequence ID" value="AAM02613"/>
        <property type="gene ID" value="MK1400"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1400"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1555"/>
    <dbReference type="HOGENOM" id="CLU_043773_0_0_2"/>
    <dbReference type="OMA" id="EVRWTLD"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01571">
        <property type="entry name" value="NAPRTase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035809">
        <property type="entry name" value="NAPRTase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="12">
        <location>
            <begin position="52"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="11">
        <location>
            <begin position="115"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AAM02613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="394" mass="43465" checksum="3C30C08858F2EA8B" modified="2002-06-01" version="1">MRLHVATEDEIRRGETADVYFRRIRKVLEEEGLADTEVVAEISTRSLPEGWEWGVLCGVEEALALLEGRDVTVYAMDEGEVFRPGQPVMRIEGPYVEFCELETALIGCLAKATGIATKAARCKVAAGGKPVYSFGIRRQHPAIAPMVDRAAYVGGCDGVSGIKGAELIGERPVGTMPHAFVLVFGDQRKAWRAFDEHVPEDVPRIALVDTMYDEVEEALMAAEELRERLDGVRLDTPSSRRGDMAEIVREVRWELDLRGYEHVKIMVSGGLDEGEIRRLAEVGADAFGVGTAISDAPAVDFAMDLVEIEGEPRSKRGKLSGAKQVWRCPNCLDDVVLPRGKEPSPCPECGSEREPLLRKFVEEGEVVREPKSPKEARRHVLETVGELEGLDLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ6</accession>
    <name>Q8TVJ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02606">
        <property type="protein sequence ID" value="AAM02606"/>
        <property type="gene ID" value="MK1393"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1393"/>
    <dbReference type="HOGENOM" id="CLU_087505_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019218">
        <property type="entry name" value="DUF2119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09892">
        <property type="entry name" value="DUF2119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005919">
        <property type="entry name" value="UCP005919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="190" mass="21830" checksum="C26B380F91EA689F" modified="2002-06-01" version="1">MSANDHPIRRPVAVYGVHGDEGRALEPVVRRVLPEGFRVSRVFENGPYVSTVDPSFHRSEVGARWRRVVEDLRPFDTYVELHCYRPRAYRKLTAERRSGGVPGLVDFGAGVLLGSVPRQHKRILGDVLTLTLEVPRRPSDRALRVVSEILGLIPGRTRGEFVKELRRRFPEATEEALRRFRSYYPDRDPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ7</accession>
    <name>Q8TVJ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized domain specific for M.kandleri, MK-33 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02605">
        <property type="protein sequence ID" value="AAM02605"/>
        <property type="gene ID" value="MK1392"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1392"/>
    <dbReference type="HOGENOM" id="CLU_1017847_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="273" mass="29792" checksum="68AB91DE3A880A15" modified="2002-06-01" version="1">MIEPYKLAWSVVFGISRGLYVFAGSFIAAALYRYVAEERITMTTAMFVGLITAGFASGPQKLAALAISQPNVEVLSWTIAALFAIPARTYGDALGKRLLEARLSSMKPTTKVYRLPEDPDNIEDVPGEPPAPREVKKRIAGREYEFPRGTPREDVERVIKRDLEEEGGVGRAVVRVDGDEVKVRLAGAKPPVSHTLPPDKVAVSVKPKGGSAHIGEGDKVIVYADGQKLCEAEVWKRSKSGVVLVVDREHADELMRLVTKGKDVSLVVEPTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL4</accession>
    <name>Q8TVL4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Precorrin-4 methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cobM</name>
        <name evidence="8" type="ordered locus">MK1375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1375"/>
    <dbReference type="EnsemblBacteria" id="AAM02588">
        <property type="protein sequence ID" value="AAM02588"/>
        <property type="gene ID" value="MK1375"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1375"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1529"/>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="OMA" id="WSAMGEQ"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="7">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="27487" checksum="A105586D68452B5A" modified="2002-06-01" version="1">MSVTKVYFVGAGPGDPELMTLKGVRVLRRADLVIYPGSLIPRESVEEWAPNAELIDSHGKTLEELVETMVEAVEDSRTVVRLVSGDPFVYSSLYEQVRELRRRGVDYEVIPGVSSVNAAAAALGEELTKPGISQTVILTRPAGRTGKPEGESLSELAEHGCTMVIFLGAAYLERIVKSLLGGAYDEDTPAAVVYKASTPEEKVILGTLGDIAEKAREEGIDRTALIIVGDVLKEEGKRSHLYSEEYARRVRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ2</accession>
    <name>Q8TVQ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-42 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02549">
        <property type="protein sequence ID" value="AAM02549"/>
        <property type="gene ID" value="MK1336"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1336"/>
    <dbReference type="HOGENOM" id="CLU_646575_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="424" mass="45818" checksum="20043939BFF3AF0A" modified="2002-06-01" version="1">MIPTLLGLLLLTTPAQAADVPADPTLGAGKVVVRFTPSSSGEWLAQAYYSPPRGKLAIVSVSGSIAGSRVELRDRYAGGPLRVSVVDVSEYAPELSQVLTPVRVERRVTREGSTYHVTVIVSNEAEHPVMVRLTYSNWTHLDFGDRTLDQDVSVVLEGEKLELEPFSVRDSRFVRADLVWTDAGGFRYPYTRLGPGDSLTLHLDVTADRSPGKVTVTRRLLLSDPPVPVSLTVSVLRPANYGLDGKKVSFWVPAPGVDYLVRYLAREGDRLVPKSLEGEGEYQGVVSVELQGEPELVSVYLKQPARMVFEVRDRVKLSLRSGSNGSSRLWVRWASESIPGGLATDVRLKGRAGRVLLGLVLDSTGEANVDVSGGTVLERGTLPSGRAYAILDAGPVNGEKVVRVTSNARVLDVLSKVVDNPGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU5</accession>
    <name>Q8TVU5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02506">
        <property type="protein sequence ID" value="AAM02506"/>
        <property type="gene ID" value="MK1293"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1293"/>
    <dbReference type="HOGENOM" id="CLU_695618_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013442">
        <property type="entry name" value="SSO1393-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09651">
        <property type="entry name" value="Cas_APE2256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02619">
        <property type="entry name" value="TIGR02619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas_APE2256" evidence="1">
        <location>
            <begin position="58"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="396" mass="43638" checksum="6E8D3F555F731A19" modified="2002-06-01" version="1">MGTSTLNNLARALGKGDLDADLDPGDAYRAARNPEAVEEIGEEVLEEALMELVEADPKRASAELNTLLRMAERAEREGLEIDSIALIPTKTANCRLCARVVAEYLREEGYAVWETEPVRAGVDPGEFWWGLSDLLERLEDVGVTDPSRNVWVAATPGFKPEAAVLTLVASLFGKPVFYVHEVMNELVEIPPAMPVVTDPGFVLGLIELRRRLGDGAPEEVAEEVLNDLARDEEERERYKLFLVEDPRSKNIGPSPLARLAESFLALGLLAAKARNYSVEIRTKGHTVPVSRGGGVKKVDRATLRELPLEDDTLEVLATVAALEGVDDTWFVAGEWTTEGTRRHARTVEVLETRDDAVVVSVRDRRVHISRLLIPTEDPERVARALKQLVGALSDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU6</accession>
    <name>Q8TVU6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotidyltransferase of the DNA polymerase beta supefamily fused to a uncharacterized conserved domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02505">
        <property type="protein sequence ID" value="AAM02505"/>
        <property type="gene ID" value="MK1292"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1292"/>
    <dbReference type="HOGENOM" id="CLU_1122627_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="121"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="247" mass="27565" checksum="B3F166812DC8E8CE" modified="2002-06-01" version="1">MRRTGPGAGAVGSVPSWEWVRRLGEAARRVLGEDARVVPFGSVAKGRAVPGSDLDVMVVSERAPSSFRERARIAYELCEEAGVPEDRVDVLIVRPKDFEVWGRMLMTSESDDTRALVEELLERGERFLRSAVESEERGWNDLAALHAHQAVELTIKAALIALGEAPPGTHFLGKLLGRLHRVTGKDAFGELSRRYRWELRELSHAWSEVRYGHYPGEDVDVGELVEVAREVVEAVRDYVLRLVDVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW3</accession>
    <name>Q8TVW3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1275"/>
    <dbReference type="EnsemblBacteria" id="AAM02488">
        <property type="protein sequence ID" value="AAM02488"/>
        <property type="gene ID" value="MK1275"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1275"/>
    <dbReference type="HOGENOM" id="CLU_105382_0_0_2"/>
    <dbReference type="OMA" id="ETAFCNA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014826">
        <property type="entry name" value="HCHO-activating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037075">
        <property type="entry name" value="HCHO-activating_enzyme_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08714">
        <property type="entry name" value="Fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03126">
        <property type="entry name" value="one_C_fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fae" evidence="1">
        <location>
            <begin position="7"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="169" mass="18286" checksum="AA16A85AD40EB087" modified="2002-06-01" version="1">MPFALRVGEAFVGEPDEPEIAHIDLVMGSVEGPVGRAFAEALASPSKGHTPLLAVLTPGVAVRPPTLITPTVTIENMAQGEKIFGPVQRGIAEAVVESVEEGIIPRDIVDDVVIIANTFVHPEAEDDRRLYENNKEAMKKAIECAMKGEPTIDELIEKKDEVEHPLAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW7</accession>
    <name>Q8TVW7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02484">
        <property type="protein sequence ID" value="AAM02484"/>
        <property type="gene ID" value="MK1271"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1271"/>
    <dbReference type="HOGENOM" id="CLU_516414_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="527" mass="56937" checksum="64796F2C40DFF8F6" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW8</accession>
    <name>Q8TVW8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02483">
        <property type="protein sequence ID" value="AAM02483"/>
        <property type="gene ID" value="MK1270"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1270"/>
    <dbReference type="HOGENOM" id="CLU_876066_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="317" mass="35193" checksum="57CDEFD1DBD92584" modified="2002-06-01" version="1">MDTGAVDDGMIVVVRSDYRTLDSDEPTWSMSYRVVRYDLVTSPRVLPVSGGPSESRSDPRMYPIGAFYDPDRDRLLVITYHPRLVLPGGDETPGVLVSEISGASRDPRFVGCRTLTITTPNDADDVKPVLPEVIDGKPYLVYVEPGEPFEEGGTREYRLVRLDLETGAREVLAEFGERTRVAGEPRTLHRLLGGSVLPRLRARLGRGRAGVRRSSRPSQAARGLDGQVLRVPATVRRRPDGRRVRRPRLDRSEPDRTRSTETRRLDRDRVAVPTTLGRPALPVDRSQVHGDGVDRGLLLGRATVRAGPGVQGDPRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ4</accession>
    <name>Q8TVZ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02457">
        <property type="protein sequence ID" value="AAM02457"/>
        <property type="gene ID" value="MK1244"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1244"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1348"/>
    <dbReference type="HOGENOM" id="CLU_1870749_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="136" mass="15445" checksum="5A11590ACC820268" modified="2002-06-01" version="1">MASRRYRYIGPCRCGLGPHAFYEDTETGEIKHAWQVLGEEGAPAPAWPGGWWRGGWRGGPGWGGGWWWLATADEETRKEVLKAIRETFGEDLAEAVEKLAESVSEGPEGGKTEELEELRKKLEELEKRLREMEEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW04</accession>
    <name>Q8TW04_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02447">
        <property type="protein sequence ID" value="AAM02447"/>
        <property type="gene ID" value="MK1234"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1234"/>
    <dbReference type="HOGENOM" id="CLU_805642_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="344" mass="38861" checksum="EFCE2347E72D0377" modified="2002-06-01" version="1">MPEIEVYEIEPERKLLELLREAPSVTEAPAWDAVYPYHRHEEGLFREVVRAVREVLYETEAARVCVGLEGMDSTLCAAAAARACEEYGVELVCYTVGFYPGMPRELVHARAYLVASELDSRYETPVYLYHLDVPPPVESKRELCRLCGRLRFEVAAQAFPGSVILGGANFGEAPHRARLDAITHHGQAIEYRPLLELGFDKGHVVAALREVNFHFPRLEWWKNESGCGVRDYLRDPDPEGVLEAAEANDTFHRILLEEGAWCGHHYDTAVVLYDGERVYPVLIPLPWGWGRDAQRAAEAAFDELADRWEVGNPEEVQEVDDPPLESILKRARQLGTVQPSTSPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW12</accession>
    <name>PUR5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="1">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purM</name>
        <name type="ordered locus">MK1226</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW12"/>
    <dbReference type="SMR" id="Q8TW12"/>
    <dbReference type="STRING" id="190192.MK1226"/>
    <dbReference type="EnsemblBacteria" id="AAM02439">
        <property type="protein sequence ID" value="AAM02439"/>
        <property type="gene ID" value="MK1226"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1226"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1329"/>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148282" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <sequence length="345" mass="37492" checksum="620F37A3B18DC964" modified="2002-06-01" version="1">MSEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLNVDGVGSKVLVAQLVGRYDTVGIDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIAEGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVACLGRVEGDPITGEDVEPGDAIVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYEVRGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGMALIVPEEEAEDVVDTVSKHVEAKIVGHVEEGRGVVVHMDGHEVKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW28</accession>
    <name>AKSA_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Homocitrate synthase AksA</fullName>
            <ecNumber evidence="1">2.3.3.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(R)-homo(2)citrate synthase</fullName>
            <ecNumber evidence="1">2.3.3.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName>(R)-homo(3)citrate synthase</fullName>
            <ecNumber evidence="1">2.3.3.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aksA</name>
        <name type="ordered locus">MK1209</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of alpha-ketoglutarate and acetyl-CoA to form (R)-homocitrate. Can also catalyze the condensation of alpha-ketoadipate with acetyl-CoA to form (R)-homo(2)citrate, and the condensation of alpha-ketopimelate with acetyl-CoA to form (R)-homo(3)citrate. These reactions are part of the biosynthesis pathway of coenzyme B and biotin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoadipate + acetyl-CoA + H2O = (R)-dihomocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:72697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:44925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoheptanedioate + acetyl-CoA + H2O = (R)-trihomocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:72699"/>
            <dbReference type="ChEBI" id="CHEBI:72701"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:44929"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="1"/>
    <dbReference type="EC" id="2.3.3.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW28"/>
    <dbReference type="SMR" id="Q8TW28"/>
    <dbReference type="STRING" id="190192.MK1209"/>
    <dbReference type="EnsemblBacteria" id="AAM02422">
        <property type="protein sequence ID" value="AAM02422"/>
        <property type="gene ID" value="MK1209"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1209"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1311"/>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="OMA" id="QIEAGFP"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044249">
        <property type="term" value="P:cellular biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901566">
        <property type="term" value="P:organonitrogen compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140412" description="Homocitrate synthase AksA">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="19"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q57926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="397" mass="43750" checksum="3D81E012BC8E9DAC" modified="2002-06-01" version="1">MQSPYVREAVREMDLPDEVIVYDTTLRDGEQTPGVSFTPEQKLEIAHLLDELGVQQIEAGFPVVSEGERDAVRRIAHEGLNADILCLARTLRGDVDAALDCDVDGVITFIATSELHLKHKLRMSREEVLERIADTVEYAKDHGLWVAFSAEDGTRTEFEFLERVYRTAEECGADRVHATDTVGVMIPAAMRLFVAKIREVVDLPIGVHCHDDFGMAVANSLAAVEAGAQAISTTVNGIGERAGNAALEEVIMALKELYGIDPGFNTEVLAELSRKVSEYSGIDVPPNKAVVGENAFRHESGIHVAAVLEEPRTYEPIDPKEVGMNRKIVLGKHTGRKAVVAKLEELGVEPEEEIVEEVLKRIKALGDRRVRVTDSKLEEIVRNVLESRGDRDDPGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW41</accession>
    <name>Q8TW41_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Precorrin isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cobH</name>
        <name evidence="5" type="ordered locus">MK1196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1196"/>
    <dbReference type="EnsemblBacteria" id="AAM02409">
        <property type="protein sequence ID" value="AAM02409"/>
        <property type="gene ID" value="MK1196"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1196"/>
    <dbReference type="HOGENOM" id="CLU_1369539_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiC" evidence="4">
        <location>
            <begin position="13"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="199" mass="20974" checksum="68D7FE60DAA18B5A" modified="2002-06-01" version="1">MTLGERTGRGRSIAERSLERWTEKLGEPENLEDLLTLRLSHATWDPFVSETLMIEPSEEAVTEGLKSAEVVAADVGMVAAGIRRSVERLGLETVIASEVGERIPEDTVTGDGMRKILEDHRNVAVVVGNAPTAAEKVAEYPESIAVAVLFPVGIGAMEAKRAAMDAGIPVVTNVSVRGGTPLAVAAFNALADCVTGNAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW46</accession>
    <name>Q8TW46_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RHH_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02403">
        <property type="protein sequence ID" value="AAM02403"/>
        <property type="gene ID" value="MK1190"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1190"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1291"/>
    <dbReference type="HOGENOM" id="CLU_1682706_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_1" evidence="1">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="156" mass="17876" checksum="09213628D803BE01" modified="2002-06-01" version="1">MHRYCHSDLIIKPYGAGRSRITVRLPGPVHRWIQREHGSVAGYVRKCLEDELTRVMLRELADSGSGRRLSTSLELPEDLVRELDEVADRLGISRNELVTCLVLREFLNEMTARRTVRSDVVCVLRELLNVLEEGWKLDLGGRRGGIEEGLRDRDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW48</accession>
    <name>Q8TW48_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02401">
        <property type="protein sequence ID" value="AAM02401"/>
        <property type="gene ID" value="MK1188"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1188"/>
    <dbReference type="HOGENOM" id="CLU_1763883_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="147" mass="15558" checksum="256A58F445974127" modified="2002-06-01" version="1">MSPHTSSAFGDPARFIIPRTGRPRPLPDGAPGTRVHPAGHRGTGRPSYSPLALSSARAVTIFSDGAYPPPPRNTNLRSECTPDTSNSAVPVHPQTSSVTTRQRPSSRTYIRTVAPGRASPVLASTTATYTRSLPAYQARILVGVFPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW69</accession>
    <name>Q8TW69_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02380">
        <property type="protein sequence ID" value="AAM02380"/>
        <property type="gene ID" value="MK1167"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1167"/>
    <dbReference type="HOGENOM" id="CLU_1149817_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="241" mass="25177" checksum="3A8ACEF5874735E1" modified="2002-06-01" version="1">MGARSSSVGSYPTDRDPAVREGAAGDRGGAPGGVRGPESVGRGPDAVWNVSDLVVEALWSSGLVYVDVSGARGPEIAGIPLGLAALEVPGLSVGGGREVRFVAGRLEDDVLTDVTEHFGAAGALIWTIICSWKPSPEAARRAIEPAERAGVDVRELDRLGEYVDALDTAQVGEVLAGTRPDRETIRTIVRILRRVLHRVYATDGRFAPPTASPLSGIIRVHSDTPTPTTCGAVTSPREPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW83</accession>
    <name>Q8TW83_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02366">
        <property type="protein sequence ID" value="AAM02366"/>
        <property type="gene ID" value="MK1153"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1153"/>
    <dbReference type="HOGENOM" id="CLU_1792137_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="144" mass="15521" checksum="C9C0A421F9585566" modified="2002-06-01" version="1">MGRVVRGLLMAAYVGLSLAITWPFAWRFFRWCAPYAPAAYTVSELVMMVGTCGILRSAIPLAAVGWGDVLNVAVVAICASYIPFCVCWIVFRVYLHCCGTALPDGGSLMTVLGAVLLSTTSFQVLWRPEGRCGSCTVRWTSAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW86</accession>
    <name>Q8TW86_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02363">
        <property type="protein sequence ID" value="AAM02363"/>
        <property type="gene ID" value="MK1150"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1150"/>
    <dbReference type="HOGENOM" id="CLU_1131614_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="245" mass="27946" checksum="B8BDFCE5DDD40F8B" modified="2002-06-01" version="1">MIYYNDATGSALGGVLMSVRTVSLLTLLLLIAPVAADIPPHASEKELEDPNWWWEHHRGPVWATRYPEVWNKIVLKYEDPEDREKVSKLGEALRHPPRGFELWIWFILGMALVLPPASRVGFLKFFKYKTWEAPEWLIQYCGTIILSMAYLAIIAMGRAYVLNCSVYYVMIGKLSEAYRTFYLWTAYAEPFLLAFNAAIPLILVSKLELPYRRLKAGILIAWLASLVLNVHMHSESLTLLSTLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW89</accession>
    <name>Q8TW89_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02360">
        <property type="protein sequence ID" value="AAM02360"/>
        <property type="gene ID" value="MK1147"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1147"/>
    <dbReference type="HOGENOM" id="CLU_1631664_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="162" mass="18609" checksum="3B203474D5C1041C" modified="2002-06-01" version="1">MRAWLLRVQAPPGQRIEKLVLLDRPPRARVWLLGLTYRVGREYHALLEPGKSELLCELKVRVKLDDQAIDLTEPSLLLIHTTRGDLEYSVIDLRNPVLPPEWKRAPPVNEITVEYGPKALRTIKLESEFYLVVLCLPPGFEYSPEDFTKWAQALAGLKDEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW99</accession>
    <name>Q8TW99_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotidyltransferase of the DNA polymerase beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02350">
        <property type="protein sequence ID" value="AAM02350"/>
        <property type="gene ID" value="MK1137"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1137"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1209"/>
    <dbReference type="HOGENOM" id="CLU_159724_0_0_2"/>
    <dbReference type="OMA" id="REIKRIC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="32"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="122" mass="14493" checksum="7E3F5696A8BA073B" modified="2002-06-01" version="1">MSGRDLYVLEARINRRNYREFVSRWREWGERIARVARRLLGEGTRVFVFGSAVKGEALPGSDLDVLIVSDGVPEDLEDRVDLIREFERELDLPERHPIEFHLATPEEYEEVWRVLLDEYEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA5</accession>
    <name>Q8TWA5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-23 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02344">
        <property type="protein sequence ID" value="AAM02344"/>
        <property type="gene ID" value="MK1131"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1131"/>
    <dbReference type="HOGENOM" id="CLU_856887_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="295" mass="32279" checksum="71F7E9ED5D281FF3" modified="2002-06-01" version="1">MSDAHELALRRRLKVDLSLLKRLGLEGSVVRNVRHPLGLLVKLAEEELVEPGRVHAHLPLYTPPISTVLRLLEASEEVGYEVTASVEVIAEDDGRVEEVREVVDRVEPVILGLPGVLTLSDRVFELASERAPLFLDLGWALHPHRAVPSVDLTTVPTLAALRAVESVSGEDAFERVVAVARLGHGLTVDRGKVRGSGGGGLKLVDVTDVLRALSVVLDLARYPVAPALGPTVRRELERLLDGFDGPEREDLTEAARAFELLSNLTPGQTEEDVKIPEHPSIIREVLEDRLRDLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC8</accession>
    <name>Q8TWC8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing two V4R domains</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02320">
        <property type="protein sequence ID" value="AAM02320"/>
        <property type="gene ID" value="MK1107"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1107"/>
    <dbReference type="HOGENOM" id="CLU_859462_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="85"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="242"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="323" mass="35360" checksum="BD1B1F202B4F40B4" modified="2002-06-01" version="1">MIDDGFSPESLRERAEEAGSVFTAEELLATNKMVMMALYSLGGERSCASVFRAGRMLAGFFGVETLEDALHTFCELTGADYDLDRNYVAIESCPECLGYVNAEGAVCNFLRGFISRAAEHELGEVVSVAQVACEATGDRRCEFILGERGEVGGFDTEVDEMTAEDIEIVMSADGRDAVEVAAFKIASEGLLRCALSDVARPMFFRAGRLYARAFINAFDPEDPDELIGHVEEISGSSYSLEGDRFIVEKCLECAGMPFKEPICHAVRGALAETLEHWNVPFKDLVEVRCAAEEDEVVGTCVVKARGVIWKAKRVVDAVKRAFH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF8</accession>
    <name>ARGC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argC</name>
        <name type="ordered locus">MK1077</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWF8"/>
    <dbReference type="SMR" id="Q8TWF8"/>
    <dbReference type="STRING" id="190192.MK1077"/>
    <dbReference type="EnsemblBacteria" id="AAM02290">
        <property type="protein sequence ID" value="AAM02290"/>
        <property type="gene ID" value="MK1077"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1077"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1131"/>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112487" description="N-acetyl-gamma-glutamyl-phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <sequence length="343" mass="37556" checksum="AD17F09826D2BB3D" modified="2002-06-01" version="1">MVVVGIVGASGYTGGELLRLLARHPEVEEVRYATSRRLEGKPVWKVHPNLRRDYPDLEFSDPDPVEIGEDCDVVFTAVPHTAAMELVPDLLEGGAVVIDLSADFRFDDVDVYEEWYGVEHAAPELNDEAVYGLPELHRDEIRRTDLIANPGCYPTGAILAAAPLVEEGLVDVVIFDSKSGTSGAGAKPSEVTHHPECAEDLTPYNPTDHRHLPEIRQELGKLGDVEVHFTPHLAPLVRGIETTAHGLGDVEIEPKELRELYVEYYDGEPFIRVCEVGEAPRLWAVRGTNYCDVGVFAVGDGRVVVASAIDNLTKGASGQAIQNMNVRFGFEETAGLEEPGYHP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG0</accession>
    <name>NPD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="1">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobB</name>
        <name type="ordered locus">MK1075</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(6)-succinyl-L-lysyl-[protein] + NAD(+) = 2''-O-succinyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">2 residues (Tyr-65 and Arg-68) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG0"/>
    <dbReference type="SMR" id="Q8TWG0"/>
    <dbReference type="STRING" id="190192.MK1075"/>
    <dbReference type="PRIDE" id="Q8TWG0"/>
    <dbReference type="EnsemblBacteria" id="AAM02288">
        <property type="protein sequence ID" value="AAM02288"/>
        <property type="gene ID" value="MK1075"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1075"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1129"/>
    <dbReference type="HOGENOM" id="CLU_023643_3_1_2"/>
    <dbReference type="OMA" id="SMQVYPA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034979">
        <property type="term" value="F:NAD-dependent protein deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006476">
        <property type="term" value="P:protein deacetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000110380" description="NAD-dependent protein deacylase">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="217"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01121"/>
        </source>
    </evidence>
    <sequence length="250" mass="27799" checksum="B051994FE5B24E05" modified="2002-06-01" version="1">MPLREAAERVVEAETVVVLTGAGASADSGIPTFRGKDGLWNKYDPRELATPEAFARDPEKVWEWYLWRRRKIAEAEPNPAHTVLARMERDGLLEAVITQNVDGLHQRAGSRRVIELHGNIWRDECVSCEYQRVNDPERGEGLEYDELPPRCPECGDPLRPGVVWFGEPLPSDALVEAENLARSCDVMLVIGTSGEVRPAADLPLVAKSCGATLIEINPSETALSPHMDVIIRERAASAMEALWNEIERLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI1</accession>
    <name>Q8TWI1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02266">
        <property type="protein sequence ID" value="AAM02266"/>
        <property type="gene ID" value="MK1053"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1053"/>
    <dbReference type="HOGENOM" id="CLU_1514624_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="18580" checksum="80534B707CBC516C" modified="2002-06-01" version="1">MPKIPVGLGYKLAYKGAKLGLWKWGTTAKTGFFSLILHSLIFGPLGVAIVIATLFHELGHYLACKLLDVPASPPVFLPLGGAFVQHGWTDPDKEVFIAAAGPFGGALAGIPLLVIYPKAAVWNGLIQLFNLLPIPPLDGSKVLRGLWMPSPTAWIGVATAVLAAALSLVGILMGLHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL6</accession>
    <name>VATA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">V-type ATP synthase alpha chain</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">V-ATPase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">MK1017</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWL6"/>
    <dbReference type="SMR" id="Q8TWL6"/>
    <dbReference type="STRING" id="190192.MK1017"/>
    <dbReference type="EnsemblBacteria" id="AAM02230">
        <property type="protein sequence ID" value="AAM02230"/>
        <property type="gene ID" value="MK1017"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1017"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1067"/>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="OMA" id="RIVKTFW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="PTHR43607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144600" description="V-type ATP synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <sequence length="592" mass="66494" checksum="3E0E1B54B395B6E1" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM7</accession>
    <name>Y1005_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative HTH-type transcriptional regulatory protein MK1005</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1005</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWM7"/>
    <dbReference type="SMR" id="Q8TWM7"/>
    <dbReference type="STRING" id="190192.MK1005"/>
    <dbReference type="PRIDE" id="Q8TWM7"/>
    <dbReference type="EnsemblBacteria" id="AAM02218">
        <property type="protein sequence ID" value="AAM02218"/>
        <property type="gene ID" value="MK1005"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1005"/>
    <dbReference type="HOGENOM" id="CLU_075726_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="Arc_TR_HTH"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000144857" description="Putative HTH-type transcriptional regulatory protein MK1005">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="128"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <sequence length="325" mass="36979" checksum="31D97A4FAE9D8BD7" modified="2002-06-01" version="1">MRAELCADLEEALRAGGHEVVRIERACFDIFVRTRDGRAYIVKVLINADGLRREVAEELRRISHFLEAVPVVVALKRHTGPLEKGVVYHRYEVPVLDPLTFARLVEGEPPKAVADRGGQYVVIRADEVDELDVSRVRRRQLRREGGRITLARAEEADVEGVPVELKTPEHVDTGRDRMTRFERRVAELLERMGAERTGKVRRAPFKLLAKDGETVLARAEEGGDRESIALRDVASATGSMGVIVTRERCKDTYVPTIDIGTLEEIKDLEDLKEYLQERDPEERVRRLVEEAGITSPREIAQRTGIRESVIREFLRRMGITDERKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN8</accession>
    <name>Q8TWN8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02207">
        <property type="protein sequence ID" value="AAM02207"/>
        <property type="gene ID" value="MK0994"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0994"/>
    <dbReference type="HOGENOM" id="CLU_971853_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="286" mass="32579" checksum="967DBF276B2FE7F2" modified="2002-06-01" version="1">MMLPKTLKPVVEDKYVESLEELADLIASADLRTGVVRITARSGDALLDAFIVVLNGKVVYIEVEEVRTGERWRGEEALEHLREILSAIEGGQSAVVDVFEASEDDIEMIFEYHGIRPKEIELEDVLPVPALEADIEFETEELEGAEKEEATEETGIGVEEIEEAYDEEPEAVESYDFTEEVEREPESLSREEILKKYGIKEPDESFVENILKEFTGVETDLRERISRVLRSYGITVFKVEGIVEVLLGNDTDEEEVVETIRDFMKDAGVDELEVRTYRPEWLGRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP0</accession>
    <name>Q8TWP0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0992</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0992"/>
    <dbReference type="EnsemblBacteria" id="AAM02205">
        <property type="protein sequence ID" value="AAM02205"/>
        <property type="gene ID" value="MK0992"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0992"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1039"/>
    <dbReference type="HOGENOM" id="CLU_021572_4_3_2"/>
    <dbReference type="OMA" id="YRQFYFR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="191"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="483" mass="54451" checksum="5081D98576B3177B" modified="2002-06-01" version="1">MIDVLLINPPDVTTKYQRFLGITAPPLGLAYIAAVLEEAGYTVKILDCPPLDMSFEDLRRAVRKLRPRIVSIMATTPIIHQAYQAAKVVKEELEDVIVCLGGYHPTFMDVECLKECPYVDVVVRREGEFTLLDLAKVFIDGVKTLSEVLGITYREKDDIVRAPDRPLIRDLDALPFPARHLLPMDKYTFFGAKTTATTMVSSRGCPVGCDFCASSAMHGHKLRMHSAERVVSEMAHVHENYGSDIIAFVDDTFTYDRRRVEEICRLIVESGLDVTWGCAARVDTIDRELLELMREAGCSVLFFGVESGSQEVLDNVGKGFTVEQTKKAFQLCREFDIVTVASAVIGLPGETHRSARQTIKFLKEIDPDYAVVSVATPYPGTKFYQEAVEKGLIEEKSWDKYTLMDPVVRTTELSPEEVKKYQKRAMIEFYLRPRYLIRRLKEDGIDAVRVTGTMIAEVAFKKMKALLAKIPSPRFSHRKGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ7</accession>
    <name>Q8TWQ7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative gamma-glutamylcyclotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0975</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0975"/>
    <dbReference type="EnsemblBacteria" id="AAM02188">
        <property type="protein sequence ID" value="AAM02188"/>
        <property type="gene ID" value="MK0975"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0975"/>
    <dbReference type="HOGENOM" id="CLU_2032880_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045038">
        <property type="entry name" value="AIG2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31544">
        <property type="entry name" value="PTHR31544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGACT" evidence="2">
        <location>
            <begin position="19"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00030602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="121" mass="13661" checksum="F84433A2FECBC226" modified="2002-06-01" version="1">MVVTPTEEEPSKDLPPERVFAYGTLTDPEMVVGVLNRLPTIIYPAVLEGYELALEDVGGRYNTIREREGSEVKGALLVGLSDEDIRAIDRYEGYPVLYERERVEVKTSLGSYEAYVYIARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ8</accession>
    <name>Q8TWQ8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Threonine synthase</fullName>
            <ecNumber evidence="5 9">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">thrC</name>
        <name evidence="14" type="ordered locus">MK0974</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="5 9"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0974"/>
    <dbReference type="EnsemblBacteria" id="AAM02187">
        <property type="protein sequence ID" value="AAM02187"/>
        <property type="gene ID" value="MK0974"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0974"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1019"/>
    <dbReference type="HOGENOM" id="CLU_028142_0_0_2"/>
    <dbReference type="OMA" id="MWGFQAS"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="13">
        <location>
            <begin position="68"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="232"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="cross-link" description="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)" evidence="12">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AAM02187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="403" mass="43382" checksum="E8D3F902A975E1A7" modified="2002-06-01" version="1">MHYVLECVECGSEFDDDSLVRCPECGGLLEVALRYDEIEVSRSTFEGRRLGVWAFRELLPVNTDDPVTMSEGGTGLHPCPRLGEELGVRELFVKNEGENPTGSFKDRGMTVGVTKAVELGAEVVACASTGNTSASLAAYAARAGLTCAVLLPAGKVALGKLAQALFHGARVIPVNGNFDDALDVVKKLADEGIVYILNSVNPFRLSGQRTIAYEICLQLDWEVPDAVVVPVGNAGNISAIWQGFLDLYRLGIIDELPRMYGVQAEGARPIVEAIRRGKEDIEPDPEPETVATAIRIGNPVNAIKALRAIRCSGGWAVEVSDEEILSAQRELATREGIFVEPASAASIAGLRKFVEEGEIDADERVVCVTTGHGLKDPETVMEVCELPEPVEPDPETIQRLIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ9</accession>
    <name>Q8TWQ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein specific for M.kandleri with repeats, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02186">
        <property type="protein sequence ID" value="AAM02186"/>
        <property type="gene ID" value="MK0973"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0973"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1018"/>
    <dbReference type="HOGENOM" id="CLU_654906_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="145"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="419" mass="44693" checksum="DFB36B26C281E70E" modified="2002-06-01" version="1">MTTENCDVESVTIELLRDGQMVDRKTVSSPQGSVEFTVTEEGEYQVRIIVECTRGVTAETLTNSVTVRFPSASVSGGLSIESVSSSSAVLKVDYSVTTEDCSVESVTVELLCNGHVIDTKTLNSPQDSVEFTVTQEGDYTARIIVQCTSGVITEAMTNTVTVRFPSASVSGKLSIKNLGASSAVLEVDYSIATENCGVNSVTIELLRDDQVVTTKTVNSPQGSIEFTVTEEGKYKAKIIARCTNGVTIETLTNAVVVQFPSTQTPAGIRFDLSILSPLAAIAGIMLAWQLLGQPVQTTSETVEAPTIPNKQVTAKEVQKEGVEQERKTTNVPETEKIKEGKELGKPLSELRSREEVEKVRGAVEVGESVRSENGSSRRRGGTTGVLVIGKYVTGIPGVLALLAGVCAVVGAALIFRCRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS4</accession>
    <name>Q8TWS4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Inactivated homologs of predicted Zn-dependent protease of TldD family (PmbA subfamily protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pmbA</name>
        <name evidence="4" type="ordered locus">MK0958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0958"/>
    <dbReference type="EnsemblBacteria" id="AAM02171">
        <property type="protein sequence ID" value="AAM02171"/>
        <property type="gene ID" value="MK0958"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0958"/>
    <dbReference type="HOGENOM" id="CLU_671960_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="3">
        <location>
            <begin position="20"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="409" mass="44102" checksum="EBBB4DBE1596B777" modified="2002-06-01" version="1">MALDDPLSQFEPGRAVLLEVETERVEVERTHDTGDRGRVSADRTIVVRVSTDGGLGVATVADPGEVDTAVERALASAEMGTSEPVEYPDSVDPARVSSCDPSAVDEDELWDLLEAVVNEVSDDVTITSVSVTGVRRRVIFRTPSDQAEREESSVTVSLDVIGEFSGFAWDTAVGPRDIDPELLAREASEMASEAPKERVSGGELAVAFHPRAFSELLTYVLIPALSGLEVLKGTSGFDRGDIGRKVGPESLRVVNDPTLDRRPGSYAFDDEGSTPKRMELISDGILRSFYTDLYSSRRLGMESTGSGLGIRRPEPSPANVIVQGDASEEEVLEEADLIVYRTLGAHTASKVSGRFSVTALWAETVEGRAVPVSVRGNLYSSLRDALISEETERTGVVEPPYALLRCRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT3</accession>
    <name>Q8TWT3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02162">
        <property type="protein sequence ID" value="AAM02162"/>
        <property type="gene ID" value="MK0949"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0949"/>
    <dbReference type="HOGENOM" id="CLU_833166_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="333" mass="37248" checksum="0C2705E4778FBB56" modified="2002-06-01" version="1">MYRGETAVGGSRVGDPWEELERFSDPELLEFSKRLDVELSGEELKATKRRVEHVESLKIKCIIVIPILALVGLAINGKMLSILASIYYEYPAYALTHAAAVLAWDVAVIYMLRPGLYRKEKRALELALRVKKPVLAYFSLYHPLTAPLAGFIILIFSSYKPLHILYKNPKGAIIMVIAYHVQIFIPLISYLVLRLTRTPVILSYSLIVTKFAVGRPVAVEKLWQEAREYRPLTLPRVAFIGVSLASTIGMLLLIGLVRLLHIEYSTKGIETIARMAPLVTLGVTPIVTVKARNLLARVPPTTIAEGILTALNVLAAFTYTIPFFVILLYYCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX06</accession>
    <name>Q8TX06_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02088">
        <property type="protein sequence ID" value="AAM02088"/>
        <property type="gene ID" value="MK0875"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0875"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.917"/>
    <dbReference type="HOGENOM" id="CLU_1363654_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="200" mass="21880" checksum="971EBADEEFB9014F" modified="2002-06-01" version="1">MPVRESLLIDTDVRRSLVLGSVLEMCEVERAAVLGTVAQGKRFEHGVFVAGKRVPGEVITVEVEVSREVVRSGSMEIDLDPADPDLVAVVADVRATGLIANLTGLRPERRGFHALFAFSGELPARVRKRVVSNLDVFRECCPCALLEIGDVKLRFLGGRQVFDERSRLSYTDVLAVRDGKPIASAWRPASRSVISKLGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX07</accession>
    <name>Q8TX07_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyltransferase (The isoleucine patch superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02087">
        <property type="protein sequence ID" value="AAM02087"/>
        <property type="gene ID" value="MK0874"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0874"/>
    <dbReference type="HOGENOM" id="CLU_1163808_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="25132" checksum="D7C849927ED6AED0" modified="2002-06-01" version="1">MQPLTRRNKDEHPAVLDPDRTIVLGDVTVSRDAVIGPEVRLGPDAVVRGAVISGRCVVYNCELGRCAVGPKTTVTGDVGDGVTIHSGTILGQLRVSGEIEPPEVGEGAFLGPGVSVLPGTVIGPESVVMPDSVVTERVPRRGVVSGNPAMPTGMVVDAETLILEFDGKRLLLEGKRGTAFDVRLSDGELEITEESPDNFGAVLEKDVVKVVRMGHAIPLPAERPFSIPLHVLEVRTKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-28" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX33</accession>
    <name>SUI1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0844</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX33"/>
    <dbReference type="SMR" id="Q8TX33"/>
    <dbReference type="STRING" id="190192.MK0844"/>
    <dbReference type="EnsemblBacteria" id="AAM02057">
        <property type="protein sequence ID" value="AAM02057"/>
        <property type="gene ID" value="MK0844"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0844"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.887"/>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="OMA" id="TIRDSWI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="SSF55159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_0000130581" description="Protein translation factor SUI1 homolog">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <sequence length="106" mass="11751" checksum="52554822D8DC9166" modified="2002-06-01" version="1">MQEDDICPVCGLPKELCVCEEVSKETVEKIKIYTEQVRPGKVVTIIEGLDDAGIDLQELASKLKRECACGGTAKEGKIILQGDHRNKVREFLIKKEGFSEDAIEVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX34</accession>
    <name>RL29_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl29</name>
        <name type="ordered locus">MK0843</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX34"/>
    <dbReference type="SMR" id="Q8TX34"/>
    <dbReference type="STRING" id="190192.MK0843"/>
    <dbReference type="EnsemblBacteria" id="AAM02056">
        <property type="protein sequence ID" value="AAM02056"/>
        <property type="gene ID" value="MK0843"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0843"/>
    <dbReference type="HOGENOM" id="CLU_158491_2_1_2"/>
    <dbReference type="OMA" id="ELRDMTP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000130512" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <sequence length="77" mass="9121" checksum="93BC421ABA302F37" modified="2002-06-01" version="1">MGKFKLRPDEIREMTPEERREKLKELKAELLREMTSKSISGVPDNPGRVKEIKKNIARILTTEREEELRKIRETEKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX38</accession>
    <name>TBP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Box A-binding protein</fullName>
            <shortName evidence="1">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA sequence-binding protein</fullName>
            <shortName evidence="1">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tbp</name>
        <name type="ordered locus">MK0839</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX38"/>
    <dbReference type="SMR" id="Q8TX38"/>
    <dbReference type="STRING" id="190192.MK0839"/>
    <dbReference type="EnsemblBacteria" id="AAM02052">
        <property type="protein sequence ID" value="AAM02052"/>
        <property type="gene ID" value="MK0839"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0839"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.882"/>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="PTHR10126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000154009" description="TATA-box-binding protein">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="94"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <sequence length="185" mass="20730" checksum="986CDA328B72E1B3" modified="2002-06-01" version="1">MEIQNIVASVDLKGEVNLDECSVILQGEYEPEQFPGLVYRLEDIGTVVLIFRSGKMVCTGAKNREQIYKSVERVREDLEKKCGVKFHGEPEVEIQNIVASIDFHVPLDLDTIAEVLVGDEDVEGIEYEPEQFPGLVLRLREPKVAMLLFYSGKAVCTGAKTEEEPEKAVKKIAEKIEKYGLKLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX42</accession>
    <name>Q8TX42_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02047">
        <property type="protein sequence ID" value="AAM02047"/>
        <property type="gene ID" value="MK0834"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0834"/>
    <dbReference type="HOGENOM" id="CLU_544701_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="500" mass="55772" checksum="9320FD2B0D02E8DD" modified="2002-06-01" version="1">MATVVMKRLIAVTLVAGLTILSFCGYESVAKAPTWKDAERALYHYGLCLVLSACSIRRVPGYSSVNILLHGYSGTSPSPVPLRWWLAWQTAKVIGLSILLSGFPALEVLKWILSGDPSRWVLAAAVLFGLVFKVRAFMSVLSQLAVTILDISEYRADKRLLQSALVSHGLRTISLLLVYLAVVLITYPVISLCLLPMIITLGWIAWYLHKMSRLPWKDHIQVCRVLKNRLRPVLVSSLLLCSVTFTLGLTTDYFTNVSVRIWEYVSGEHVRTVHTLVPAVLLDQTSLRRIAETHRADMSFLTLDVLRDRVVVLIGGESGQATAIDPRTGRSLGSQKLPPELRESSVEALTEDLEYAYPYLRAGLPVLYSGKLTVSGWVVADVWIPFAGYTLMIPEITLEVRGRGFKPTEAPAFPGVDWGITWYPARLDGRIVPVVIPSTVCFTSSIRVPGSLVIPLYGPTAVRVWLHQPCFIEIERDRVYVLKPEYYPDGILMKVEKIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX59</accession>
    <name>Q8TX59_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sugar kinase of the ribokinase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0817</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0817"/>
    <dbReference type="EnsemblBacteria" id="AAM02030">
        <property type="protein sequence ID" value="AAM02030"/>
        <property type="gene ID" value="MK0817"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0817"/>
    <dbReference type="HOGENOM" id="CLU_027634_2_2_2"/>
    <dbReference type="OMA" id="ANVACWA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="5">
        <location>
            <begin position="6"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="297" mass="32341" checksum="D75671B885314E79" modified="2002-06-01" version="1">MLDVVGLGALNVDELLYIPRMPERDDSVPVERRVRRGGGSAANTICWLAHLGREVGFVGKVGSDDAGDLLLREFEEYGVDTSRVVRGDGHSGTAFCLVSGDDRRILVDPGVNDELRPDEVDLDYIRKARVLHTSSFIGLRSETSLETLKRTMKAVADELMVTFSPATMVLRGWSYLEPYFEAADVVFLNETEAVHLTGDIEETLNRLAELVEVTIVTRGSDPAIVQEGTEISEVAPEPVPEEDIVDPTGAGDAFAAGFIEGILRGEPADRCCERGHAVAAECLRIEGCRPPTEGRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX61</accession>
    <name>Q8TX61_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Small-conductance mechanosensitive channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mscS</name>
        <name evidence="7" type="ordered locus">MK0815</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02028">
        <property type="protein sequence ID" value="AAM02028"/>
        <property type="gene ID" value="MK0815"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0815"/>
    <dbReference type="HOGENOM" id="CLU_1136064_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="26714" checksum="260F5BA6DDC3D376" modified="2002-06-01" version="1">MELPSMKLPSAHVEPLKLATAIVIALLLVKIMGRAARAVRGKLEEEDLQDLGWWVEKTLLYGSYLLAFSIVLESLGVSLWALVTGLGLAGAGIAVAARDLIANLLAGLYLALERPFEVGDRIRVGEYSGEVVDLRIRCTVLKSRGRRIVIPNSVLVNETVEKLDDSDECEFEVVVEGSPGEIGRRLAALEKELDSLKLKYYEISVERVESGRTLVRVRTAGKEVDSVHGAVRRALVKRPGGTDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX86</accession>
    <name>Q8TX86_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Possible metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02003">
        <property type="protein sequence ID" value="AAM02003"/>
        <property type="gene ID" value="MK0789"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0789"/>
    <dbReference type="HOGENOM" id="CLU_971852_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="286" mass="32192" checksum="A586F76C862B1C54" modified="2002-06-01" version="1">MGTPDEALQDRDESPERVMELEVLVLSPAALYRRTVKALRDLTGFAREVVGLEEFGVGIVENPREYVRWLAKRVDEGTAEDALLTLVGGSGALVVRVGGDTYHVLIGPAYRSEESVKAALAHELAHLLQHRLKLFPKDVPRPAGAVRSVLLPLEVGAEELVLRELPEIGEVRIRMAQEEAREWRRTGDPILDLENALVAVPIRLAARRLGLDTSVQEPSIEDPELREVKEELVKLARGVDPWDQSDVERYTRKSVSILLDWLLDQDRHHSTSDSNNRKPSSEFRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX96</accession>
    <name>Q8TX96_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0779</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01993">
        <property type="protein sequence ID" value="AAM01993"/>
        <property type="gene ID" value="MK0779"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0779"/>
    <dbReference type="HOGENOM" id="CLU_920132_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="26"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="302" mass="32809" checksum="CFA24BADF960D1A3" modified="2002-06-01" version="1">MREEVASSPQGLGLEILAKHKVSDFVRHGAIAVKPDEPLWNALKAMVTHGIHGAAVMEDSKIVGAFEEDDLLRALRALEEDALAAEVKRFMKPAVVVKSSDTFQNAMVEMLKGNTTRAFVRSSGLLRSGVYGVISASDIVRVLTGDYRGFRAPGNTDRPVSPPKVGDIFWPGSVAIKQVIRNSPLTLDASSSVADVARCISEKGRHYAVLLEGESPIGRAGDKDVMGAALDAILNRRDLHDLTARNYLQEMVVVPPETPLHEALWEVIDKMSDRIYVMDGRKLTGVVPLIDAVYTLAKVASD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA7</accession>
    <name>UPPS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="1">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="1">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="1">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppS</name>
        <name type="ordered locus">MK0767</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXA7"/>
    <dbReference type="SMR" id="Q8TXA7"/>
    <dbReference type="STRING" id="190192.MK0767"/>
    <dbReference type="EnsemblBacteria" id="AAM01981">
        <property type="protein sequence ID" value="AAM01981"/>
        <property type="gene ID" value="MK0767"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0767"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.807"/>
    <dbReference type="HOGENOM" id="CLU_038505_2_0_2"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123732" description="Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <sequence length="271" mass="32077" checksum="FFFC36244F2C4EAE" modified="2002-06-01" version="1">MKRVTGVNSRRVLRQAVRVSLKPVYKMYERILEEKVKEGRVPEHVGIIMDGNRRFARELGLEPWEGHRYGADKLEDVLEWCLDLGVKAVTVYALSTENLNRPKEELKRLFDLMEERFKALAESERIHRRKVAVRAVGRLHLLPTRVRRAIKKAERATKEYKDRFLNVAVAYGGRQEIIDAVREIAHDVKVGRLDPDEIDEGTFRKYVYVGDLPDPELIIRTSGEERLSNFLLWYSAYSELYFVDVYWPEFRKIDLLRAIREFQKRERRFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD9</accession>
    <name>Q8TXD9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Orotidine-5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="2">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pyrF</name>
        <name evidence="5" type="ordered locus">MK0735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0735"/>
    <dbReference type="EnsemblBacteria" id="AAM01949">
        <property type="protein sequence ID" value="AAM01949"/>
        <property type="gene ID" value="MK0735"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0735"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.775"/>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="OMA" id="EMSHPGA"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="218" mass="23222" checksum="0A9B85235D2CAA66" modified="2002-06-01" version="1">MLEPDTRLIVALDVSPDRAVEIAETLDGYVDGFKVGYEVVLAEGAAGIERVAEVLEESFLLVDLKTADIPEISRSIVERVSEAGANAAIVHGFVGPDVVEECAEVLPVFVVATMSHPGAREFYDRACRDIVKACDDIDGVVGYVAPATRPERVREVRKITDKLIASPGVGAQGAAPGDAVRAGADFEIVGRAITEAHDPVEAAKDLTRAMRTSRPAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE0</accession>
    <name>Q8TXE0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01948">
        <property type="protein sequence ID" value="AAM01948"/>
        <property type="gene ID" value="MK0734"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0734"/>
    <dbReference type="HOGENOM" id="CLU_2968475_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019300">
        <property type="entry name" value="CooT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10133">
        <property type="entry name" value="CooT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="58" mass="6578" checksum="6C923E45EB685CB9" modified="2002-06-01" version="1">MCEFTVKLGGEVIAEDVLYLEVTEEGVVLRDVMGEEQVIEGAQVKRIDMDEHVVELER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE5</accession>
    <name>Q8TXE5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pyruvate:ferredoxin oxidoreductase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">porB_2</name>
        <name evidence="2" type="ordered locus">MK0729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0729"/>
    <dbReference type="EnsemblBacteria" id="AAM01943">
        <property type="protein sequence ID" value="AAM01943"/>
        <property type="gene ID" value="MK0729"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0729"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.769"/>
    <dbReference type="HOGENOM" id="CLU_048564_2_0_2"/>
    <dbReference type="OMA" id="SAWIASP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="56"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="271" mass="30022" checksum="B9D0260F1B188D69" modified="2002-06-01" version="1">MTVVDWKELVRWDRMPHILCPGCGNGTILNALVRVLAEKFEEGELDPDKTVLVSGIGCSSRLPGYVKLDSLHTTHGRPLAFATGIKLANPDLEVIVITGDGDAAAIGGNHLIHAARRNLDVTVICANNYIYGMTGGQVSPTTPRGAKSTTTPYGNPEPPFDLCELVMGAGAPHVERWTTAHPAQLKAAIARALEREGFSFIDVLCQCPTNYGRRNDMRDPREMVEWLRENTSTREEEGKIRIGILRDEEREPFHKRMYDMIEEVRTGEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE6</accession>
    <name>Q8TXE6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate:ferredoxin oxidoreductase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">porA_2</name>
        <name evidence="3" type="ordered locus">MK0728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0728"/>
    <dbReference type="EnsemblBacteria" id="AAM01942">
        <property type="protein sequence ID" value="AAM01942"/>
        <property type="gene ID" value="MK0728"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0728"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.768"/>
    <dbReference type="HOGENOM" id="CLU_017038_0_1_2"/>
    <dbReference type="OMA" id="DFIQGDE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="PFOR_II" evidence="2">
        <location>
            <begin position="277"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="372" mass="41157" checksum="EC141D00203839CB" modified="2002-06-01" version="1">MGKVDFLQGNEACAEGAIAAGCRFFAGYPITPSTEIAERMSARLPEVGGVYVQMEDEIASLAAVIGASWAGVKAMTATAGPGFSLMQEHMGYAVMAETPCVIVNVQRGGPSTGQPTKASQSDVMQARWGSHGDYEIVALSPSTVQEMYDLTIECFNWSERLRVPAVLLTDEVVGHMRERVVLRDDVETVERELPPEGEEIEKPFPMEPDDLVPPMPVFGRGHRVHVTGLTHDERGYPATDDPEVHRKLVMRLCNKVRKRAREIHEEVGYDFEERESDVALVAYGGCARTVIEAAEELGVTVFRPKVVHPFDPDMIRDLLDGYEVLVVEMNLGQYVEMVERALDRECVHLLGYPGGYPPTPERVIREVKKLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG1</accession>
    <name>Q8TXG1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="6" type="primary">dfp</name>
        <name evidence="3" type="synonym">coaBC</name>
        <name evidence="6" type="ordered locus">MK0713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0713"/>
    <dbReference type="EnsemblBacteria" id="AAM01927">
        <property type="protein sequence ID" value="AAM01927"/>
        <property type="gene ID" value="MK0713"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0713"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.754"/>
    <dbReference type="HOGENOM" id="CLU_033319_0_1_2"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="3"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="DFP" evidence="5">
        <location>
            <begin position="167"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="172"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="376" mass="41101" checksum="440A48FA473EAA67" modified="2002-06-01" version="1">MVNVVVCVTGSVAAYRAPDVCRELVRRGHRVRVVASEEALRFVGKDALGFAAEEVIFRLTSRAEHVELAEWADVVAVVPATLNTLAKIARGVADAPVPLTAVTSLGAGKRLVVAPAMSLHMYRSPPAREILRQLEDMGVTVVGPVIEEGKAKLASIEEIVEAVEGELSGFRVLVTGGPTMEPLDDVRVITNRSSGRTACEICRELRARGAEVVFVHGPLRVDPPPMDERVEVETTREMLEEVVKRIDDVDAIVMAAAPSDFRPAERADGKLDSRREHEIRLIPTEKIVREVFPDFDGVRVAFKLDPEPVEGARRLLDEVPCELVVANPPETAGAEGSEWWILDGDGEVIERVKGDKRELARKLVDALSKLLRGDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI1</accession>
    <name>Q8TXI1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01907">
        <property type="protein sequence ID" value="AAM01907"/>
        <property type="gene ID" value="MK0693"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0693"/>
    <dbReference type="HOGENOM" id="CLU_709061_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="2">
        <location>
            <begin position="75"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="389" mass="44121" checksum="7C692E404B9B1E15" modified="2002-06-01" version="1">MSGELDRFLERGRVRRGRDYGRLLEDISRRRRELAEDVINVYRRVSPGRNPVLRRVLEVVAERKREVEPEHPDPEVTFGVDGGEGMREYQGVVLYVTRAAAWSEADVLSSWDFGVLSRTRTPQMRVAARRVKLESDVATRAVERGGEVVMLDGPIVPHHDLKGANEDSPNRRDYWGRILDARRTMLEVCEEEGAVVMGIVEDCKAKHLLRDVRDELGEDLPLKALPNDPVALSRELDGGPVLEVGERTHAFELPDEDYPVVREFEEATGYSIFTFYVRTTRYSPPVRVEIPEFVDPDEAAGIVLGTSIEYGGYGIPLPLVMADEFAKVGRSLVDWIEEEVLTELASDGRFDVIFTVFRRLRRESRPKRRSSAEAEREIHLRHGGEEGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM8</accession>
    <name>Q8TXM8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01849">
        <property type="protein sequence ID" value="AAM01849"/>
        <property type="gene ID" value="MK0634"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0634"/>
    <dbReference type="HOGENOM" id="CLU_1149814_0_0_2"/>
    <dbReference type="OMA" id="AFDTYGG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012021">
        <property type="entry name" value="UCP005278"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005278">
        <property type="entry name" value="UCP005278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="27415" checksum="6DD57268B786E95A" modified="2002-06-01" version="1">MRRLPRGVLCVGLRAVAFSADLSEDDVRVSRGLLRRVERDLKELRRGFRDELMYVVLGPVCGDDLAVVYVVEEGALEDVNAAVFDLFREYGGEDVMGVSESPEGAGEGPSYAEAACPESEYQDVVVTLFDTYAAENRVAEVAEVCRRAAEGLCYDVGGGPVQEPMEIPGVGYVGPETDDPVLIATTERLDQVGPTAGAILGAGRGAGARPVRRGAPAEVLPGTVIFSVAIVLNGNVIDGVRALEEGTGTERWPLRYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ0</accession>
    <name>HPRT_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hypoxanthine/guanine phosphoribosyltransferase</fullName>
            <shortName evidence="1">HGPRTase</shortName>
            <ecNumber evidence="1">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hpt</name>
        <name type="ordered locus">MK0610</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXQ0"/>
    <dbReference type="SMR" id="Q8TXQ0"/>
    <dbReference type="STRING" id="190192.MK0610"/>
    <dbReference type="EnsemblBacteria" id="AAM01825">
        <property type="protein sequence ID" value="AAM01825"/>
        <property type="gene ID" value="MK0610"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0610"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.647"/>
    <dbReference type="HOGENOM" id="CLU_126376_0_0_2"/>
    <dbReference type="OMA" id="FIHPISD"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149497" description="Hypoxanthine/guanine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <sequence length="187" mass="20687" checksum="E1C093351104B8AD" modified="2002-06-01" version="1">MLLEKSLEEAPVVNRNGYWYFIHPITDGVPELPPELLREVAYRIVRALDSTDFDKIVCVEAMGIHLGALLSDMLDRPLVIVRKREYGLDGEVEITQEKGYGVEKLYLNGVSEGDRVVVVDDVISTGGTLVGLINALDDVGAEIEDVVVVVARGGLDRVREETGVDVKYLVRVEVSEDGVSVVESRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS5</accession>
    <name>Q8TXS5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0585</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01800">
        <property type="protein sequence ID" value="AAM01800"/>
        <property type="gene ID" value="MK0585"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0585"/>
    <dbReference type="HOGENOM" id="CLU_640323_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="428" mass="48745" checksum="964D477CA264D13C" modified="2002-06-01" version="1">MSRGTKAGIYRDVLVIEVFPGAVPDTGTLRALNERERRLLLAAAGYDVTEFWARSFIGLLFDHPTPLVRASERHEKLLPEATVAAVRELCDRIEPEKLRKIRSRVASGETPREAVRRVLGSKMAGAVSRLLGRVMGYVQSDPLSVAITLQVLGTMIAADKRLEDVSTVLVVVPGLEHVHIDVYRLARRVYSNLVRAMKSLRRYLNPRVRVRVYVATGGKLEHPAGDSPEEELIEAYEELSRRPTVRRFFPERGHFTEVPWDRALAEFFVRVIRRELHRRSLKVVRAAKREGASGRELSEVYGREYGKWLSRWFKRGRRWLNFHRPSPALSDISCCFVASEAGVEGYHEEFLIKVLQKVLREVVERRVTGRRLVRKIVRKIARVLKEDFRVEGKIREGILHMARLLGGRRKAALGGKTLLEAFGAPPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU7</accession>
    <name>Q8TXU7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01777">
        <property type="protein sequence ID" value="AAM01777"/>
        <property type="gene ID" value="MK0562"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0562"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.597"/>
    <dbReference type="HOGENOM" id="CLU_1340739_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="23239" checksum="342C24663D37C8CA" modified="2002-06-01" version="1">MILRPSTRPAERALDALLEELDTMIEACDLLSDVTTMEESPKEVEERQESLSEEVAELKDRVDEAMESSSMIPVVKEELGYIAENVRRAAETLLSLASLLVSFYEDLEEEDREKLDEVIEGIAKIASGVRESVELLNEDVRKAAEHVEKIRDQEEDVRSRLRTLMLKTERNGRLAPIVLEIERALEDMDRALDGVNRIKVRFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX7</accession>
    <name>Q8TXX7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01747">
        <property type="protein sequence ID" value="AAM01747"/>
        <property type="gene ID" value="MK0532"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0532"/>
    <dbReference type="HOGENOM" id="CLU_113705_0_0_2"/>
    <dbReference type="OMA" id="NFEGECK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="2">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="62"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="128" mass="14665" checksum="5701309A33D21216" modified="2002-06-01" version="1">MDFEEYLEKWFKGETTEVGIYMAMAIVAREQGYPEVCDLLEQIAMDEARHAAAAALIAGKVENSLEGLIEKMEEMIEGEKNAYETRMDEADHHSDELDDEIITLLKATAYDEKHHRKMLKAALEKLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-06-13" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ2</accession>
    <name>SYY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="1">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tyrS</name>
        <name type="ordered locus">MK0516</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXZ2"/>
    <dbReference type="SMR" id="Q8TXZ2"/>
    <dbReference type="STRING" id="190192.MK0516"/>
    <dbReference type="EnsemblBacteria" id="AAM01731">
        <property type="protein sequence ID" value="AAM01731"/>
        <property type="gene ID" value="MK0516"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0516"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.549"/>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_2"/>
    <dbReference type="OMA" id="YIGFEIS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02008">
        <property type="entry name" value="Tyr_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023684">
        <property type="entry name" value="Tyr-tRNA-ligase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000240255" description="Tyrosine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="215"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02008"/>
        </source>
    </evidence>
    <sequence length="324" mass="37152" checksum="5F5B99D69FF3CAF8" modified="2002-06-01" version="1">MDVEERLKLVTRNAVEVVTEEELRQLLEEKEEPVAYVGFEPSGKVHLGHKLVIDKMIDLQEAGFHVIILLADLHAYLNEKGTLEEVRELADYNRRCFLAMGLDPNKTEFVLGSEFQLDEDYALDVYRMARHTTMRRARRSMDMIARSEENPPVSQVVYPLMQALDIVHLNVDLAVGGLEQRKIHMLARDVLPKLGYDSPTCLHTPIIHGLDGDEKMSSSKNNFIAVDDEPEVIREKLRKAYCPAREAEGNPILEIYRYFIFREYDEVTIERPEKYGGDVTYTSYEELERDFVDGELHPLDLKENAAGYLSEILKPVRKAVSAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ7</accession>
    <name>Q8TXZ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted Zn-dependent metallopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01725">
        <property type="protein sequence ID" value="AAM01725"/>
        <property type="gene ID" value="MK0510"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0510"/>
    <dbReference type="HOGENOM" id="CLU_638752_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="47468" checksum="78CCB42FDAE1544C" modified="2002-06-01" version="1">MSRLYSIVAVATMVLLTGAAADLQEYKEAYSAAAKIEEYGEKVKESLNIIGGFQPPCGLKVIPTAYPYMAFKDDKDAISKVFQGTDVAESGSWTEVGPGDFRALIYGIHELGENWFERVKVVADLAQKPADYALKVHEETHAWEGAVEKVGETWEDWENHKGDENLKTAIASRVLAVLGLVVSENWRAIFNEVNKAAQIAENVGRVTIPAGVYGNDSPIELDFSKCESPEDVVNVIKAAFGDLTPDRVIELIDSEAKYFGLGDKFVENYMEGGQYQPHEGGFTVDEMKDVIEKFKSGKDIYGQELSEEALAFAILFNIEDVWATYEEYVHQYGHFFPPAESDLKLACDKIEENAKIVEDVLNKLNAWADVGITKAEFESNPLECAPAVKDWAVGELFGVVEKLEKRKIPLSPVVVLAGLLAALAVLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY23</accession>
    <name>Q8TY23_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="2">DAP decarboxylase</shortName>
            <shortName evidence="2">DAPDC</shortName>
            <ecNumber evidence="2 3">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 6" type="primary">lysA</name>
        <name evidence="6" type="ordered locus">MK0483</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="2 3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0483"/>
    <dbReference type="EnsemblBacteria" id="AAM01698">
        <property type="protein sequence ID" value="AAM01698"/>
        <property type="gene ID" value="MK0483"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0483"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.513"/>
    <dbReference type="HOGENOM" id="CLU_026444_0_0_2"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022643">
        <property type="entry name" value="De-COase2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00278">
        <property type="entry name" value="Orn_DAP_Arg_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn_Arg_deC_N" evidence="5">
        <location>
            <begin position="30"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Orn_DAP_Arg_deC" evidence="4">
        <location>
            <begin position="274"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="266"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="405" mass="45467" checksum="432B160CC15A8BD2" modified="2002-06-01" version="1">MVESSTTSWRRPVRGHRCTSTTRMLPVKAAREYQEAFSWFPDRVYVCYAMKANFNPYLVEHIVDETRAADVVSLWEMKVAVNAGAETVVVNGNAKSSDEIRAAVERSWVINVDSFEEFQRIEKIARHEGERALVALRVNPKVSPDTHSHIATAVEGSKFGVELEIAERVCRRMIESEWVEFLGLHYHIGSQITDLRPFSEALRSVRTFLEDTGLIEEISYLNIGGGLGIRYRRGEEVPSPHDLAEELQEDLKELHSESSGFDLYLEPGRSIVGEAGILVTSVRQVKRGRRRWVFVDTGMNALIRPALYDAYHEVVVHGGDYSATEKASVAGPLCESGDVLAEDRELPIDISEGDLVVFLSAGAYCESMASNYNCYPIPGSVVVRNGEITGVRRVQDYEAFSKTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY31</accession>
    <name>Q8TY31_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaC</name>
        <name evidence="2" type="ordered locus">MK0475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0475"/>
    <dbReference type="EnsemblBacteria" id="AAM01690">
        <property type="protein sequence ID" value="AAM01690"/>
        <property type="gene ID" value="MK0475"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0475"/>
    <dbReference type="HOGENOM" id="CLU_174513_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019214">
        <property type="entry name" value="DUF2109_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011316">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09882">
        <property type="entry name" value="DUF2109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036534">
        <property type="entry name" value="EhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9526" checksum="C257CEFAD8A480AB" modified="2002-06-01" version="1">MISAQAVMAAIVILMSLRLLVARDLYAQMLYLNVVGFGLAGLVATTWRTDMGLIAALCVFIFSTLESNAVSYTIQKIEEIRRAGELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY46</accession>
    <name>Q8TY46_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaP</name>
        <name evidence="2" type="ordered locus">MK0460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
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            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01675">
        <property type="protein sequence ID" value="AAM01675"/>
        <property type="gene ID" value="MK0460"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0460"/>
    <dbReference type="HOGENOM" id="CLU_097041_1_0_2"/>
    <dbReference type="OMA" id="VLCGHCE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="85"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="147" mass="15793" checksum="92DA4469DD85A5F5" modified="2002-06-01" version="1">MDVIVEVREVVVIHELCRACGLCEKECPTGAIEVEDSAKIDEKDCVRCGLCVEVCPFDAILLGRATCELPKGSYRIEVLTKRPEVSVRISESKCVGCQACSSSCPVEALFGAKGSPPKLDVDRCVGCLECVRICPSRAIEPVGGFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY65</accession>
    <name>Q8TY65_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01655">
        <property type="protein sequence ID" value="AAM01655"/>
        <property type="gene ID" value="MK0440"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0440"/>
    <dbReference type="HOGENOM" id="CLU_1590900_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="19211" checksum="C8A9ECF22AB65BDF" modified="2002-06-01" version="1">MRPLPLIVACAVALASPALAWERTEVSVDTYVLNYEGDAPNHLMLDVDIADENGNVFVGWLLDGLESKSEPRVWEGLMDRVTVILNAIYDDGKLRLEMRKTFTVHFDYDGNDGVHVYVKGDDAPIIRTEDGRTVLVWERCQRRRIRSSEDNGRRCERSEMEGRTWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY81</accession>
    <name>Q8TY81_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0424"/>
    <dbReference type="EnsemblBacteria" id="AAM01639">
        <property type="protein sequence ID" value="AAM01639"/>
        <property type="gene ID" value="MK0424"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0424"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.453"/>
    <dbReference type="HOGENOM" id="CLU_060249_0_0_2"/>
    <dbReference type="OMA" id="HAFTPWT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00375">
        <property type="entry name" value="TIGR00375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="417" mass="47395" checksum="6C00E0307784E475" modified="2002-06-01" version="1">MGEQYDSDLHLHSQYSGGTSPRMVIREIARGAAKKGLDLVGTGDILHPKWRRHVRRELVEDEYGLLKEPKTGVLFVPTVEVEDERRVHHLIILPSLDHAEELHGELSRYSDDIDAEGRPHLRMTGAELADLLKDHDCLFGPAHAFVPWTSVFKEYDSLRECYGSAMDRVDFVELGLSADSDYADRISELHEYTFLTCSDAHSPYPHRLGREFVRFELEEPSYDVLKAAIRRKPGGRVVLNVGLIPELGKYNRTACARCKRQFELEEAERLNWRCPECGGTIKKGVRDRVLELADLEKPKHPNHRPPYLRIIPLAEIIAKALGLSTITAKKVRAVWNSLVRRFGSEIDVLIETPIEEIAEVDERVAELLKSFREGTVNIRPGGGGEYGKIITEEESEREEPRSRKPVQRTLDELIGRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB1</accession>
    <name>LEU1_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Probable 2-isopropylmalate synthase</fullName>
            <ecNumber>2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuA</name>
        <name type="ordered locus">MK0391</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYB1"/>
    <dbReference type="SMR" id="Q8TYB1"/>
    <dbReference type="STRING" id="190192.MK0391"/>
    <dbReference type="PRIDE" id="Q8TYB1"/>
    <dbReference type="EnsemblBacteria" id="AAM01606">
        <property type="protein sequence ID" value="AAM01606"/>
        <property type="gene ID" value="MK0391"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0391"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.417"/>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140451" description="Probable 2-isopropylmalate synthase">
        <location>
            <begin position="1"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="499" mass="54339" checksum="B14899946601AF17" modified="2002-06-01" version="1">MPDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGEFEAVRAIAGEELDAEICGLARCVKGDIDAAIDADVDCVHVFIATSDIHLRYKLEMSREEALERAIEGVEYASDHGVTVEFSAEDATRTDRDYLLEVYKATVEAGADRVNVPDTVGVMTPPEMYRLTAEVVDAVDVPVSVHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIGERAGNASLEQVVMALKALYDIELDVRTEMLVELSRLVERLTGVVVPPNTPIVGENAFAHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVTEEQLDEIVRRVKELGDKGKRVTEDDLEAIARDVVGEVPESEAAVKLEEIAVMTGNKFTPTASVRVYLDGEEHEAASTGVGSVDAAIRALREAIEELGMDVELKEYRLEAITGGTDALAEVTVRLEDEDGNVTTARGAAEDIVMASVKAFVRGVNRLARRRRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC5</accession>
    <name>BRIX_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0377</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC5"/>
    <dbReference type="SMR" id="Q8TYC5"/>
    <dbReference type="EnsemblBacteria" id="AAM01592">
        <property type="protein sequence ID" value="AAM01592"/>
        <property type="gene ID" value="MK0377"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0377"/>
    <dbReference type="HOGENOM" id="CLU_107897_1_0_2"/>
    <dbReference type="OMA" id="VKIWIME"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000120271" description="Probable Brix domain-containing ribosomal biogenesis protein">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <sequence length="192" mass="21528" checksum="C6E605A3D553C84C" modified="2002-06-01" version="1">MRPAAITTSQRPARRTRSLCRDLECALPDATYVLRGTKNLRDTVLEALESGAEVLFYVTEAKGNPARLHVIDLGEIPPRLRLSFWLGGVKLQRELFGNRVDLSGDLVITTSKRPVSGHMKVAESLSEVLGVEFVPRAGSLEDVLEEALADVLLVVEGHPRHLGTLTFYRRTEKVGPSLFYRDFRTKDERMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI1</accession>
    <name>Q8TYI1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted DNA methylase containing a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0318"/>
    <dbReference type="REBASE" id="6879">
        <property type="entry name" value="M.MkaORF317P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01533">
        <property type="protein sequence ID" value="AAM01533"/>
        <property type="gene ID" value="MK0318"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0318"/>
    <dbReference type="HOGENOM" id="CLU_1363652_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="200" mass="22636" checksum="AC0365C730E83065" modified="2002-06-01" version="1">MGVFWTGAGGPVRVVGELVAEDLVPTEEIPKYDSERVLSKGVDRFYKLFNERQLLVHAELLGVIRELCEGLDEEYREPLTVYAMIAFDKMINYNTICSRWHYGRGAVVGIFDQHAYAWSWDHGEMGAVPGWWDFGVKGVLEAYEEFIRLLRGVKERPRVILGDARKLPTLLEEKPDTIVVDPPYYTTSSTPSSPTSSTCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK0</accession>
    <name>Q8TYK0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0297</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0297"/>
    <dbReference type="EnsemblBacteria" id="AAM01514">
        <property type="protein sequence ID" value="AAM01514"/>
        <property type="gene ID" value="MK0297"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0297"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.301"/>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_2"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="ACT_dom"/>
    </dbReference>
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        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="450"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="522" mass="57776" checksum="5580015BF0A36CDA" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM4</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01489">
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        <property type="gene ID" value="MK0272"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0272"/>
    <dbReference type="HOGENOM" id="CLU_1507390_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="178" mass="19868" checksum="78BD36F02CD8B24B" modified="2002-06-01" version="1">MFSGIREGAVQVPKWILDVLTGKKLRVHELRAPVWVGHRELMLLSMDVRDDENPLSLTAGVLGLFSAALADVFTWSRGSELSYPLTSANGDAWRVCVDAAVLAVIDAFSSELEVSVEAFVSEAGIPWLATLANNGFRPESVIRGTDEVFVATLLNLLKYEVKHRCGDRWRDVWRRLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM6</accession>
    <name>Q8TYM6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="16739" checksum="ACC0B773F66BDAA3" modified="2002-06-01" version="1">MILKFDHWSGPLYLHCSIRRGDPRRIIERSSTGVIIQAISTEYADYRVIASAACRAIRAFESDRNLARSLDLEFLVRLTGTRQIREAIDRAEPGDEFVLVVASRDRKKVRGVLKELEEEAEELEEFPERDGYKELLRTAASVDAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN5</accession>
    <name>Q8TYN5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Formylmethanofuran dehydrogenase subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwdB_1</name>
        <name evidence="2" type="ordered locus">MK0259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0259"/>
    <dbReference type="EnsemblBacteria" id="AAM01476">
        <property type="protein sequence ID" value="AAM01476"/>
        <property type="gene ID" value="MK0259"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0259"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.262"/>
    <dbReference type="HOGENOM" id="CLU_034348_0_0_2"/>
    <dbReference type="OMA" id="VCIDPHE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin" evidence="1">
        <location>
            <begin position="52"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="434" mass="48717" checksum="1D1876BEF78F336E" modified="2002-06-01" version="1">MGRKVIKDVVCPFCGTLCDDLEVVVEDGEIVEVRHACRIGAAKFLTAQEDHRHTEPMIKENGEWKKIDYEDAAEETARILVEAKWPLMYGWSSTLCEAHEVGIEIAEKVGAVIDNTASVCHGPSTLGLQDAGVPSCTLGEVKNRADVIIYWGCNPMHAHPRHMSRYTAFTRGFFRPKGREDRTIIVVDPRRTATAELADVHLQVRPNEDYELISALRAAVHGIEIEREEVAGIPVEAVHEVADLIKEASFGTLFWGMGITMSRGRHRNIDNAICLIRDLNEYAKWTLIMMRGHYNVTGFNEVLTWTTGYPFAVDFSRGYPRYNPGEFSAVDVLIRGEVDAAFVIASDPGAHFPRKAVEHMARIPLVCVDPHWTPTAELADLYVPVTIAGIEWEGTAYRMDSVPIRMRKVVEPPESMLNDVEFLEMVIEKVEEMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV6</accession>
    <name>Q8TYV6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0185</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01402">
        <property type="protein sequence ID" value="AAM01402"/>
        <property type="gene ID" value="MK0185"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0185"/>
    <dbReference type="HOGENOM" id="CLU_2678945_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="74" mass="8144" checksum="8CCB8A42632041B4" modified="2002-06-01" version="1">MIRSLRTLKRAVVVNPLNPQSLRVLAVEVASDVVTAMAFSVRFSLLWIQCAPHGRDDAVEGAAGYGESLKGRYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW6</accession>
    <name>Q8TYW6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mn-dependent transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">troR</name>
        <name evidence="6" type="ordered locus">MK0175</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0175"/>
    <dbReference type="EnsemblBacteria" id="AAM01392">
        <property type="protein sequence ID" value="AAM01392"/>
        <property type="gene ID" value="MK0175"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0175"/>
    <dbReference type="HOGENOM" id="CLU_069532_3_0_2"/>
    <dbReference type="OMA" id="SWDAIDR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="SSF47979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="158" mass="18426" checksum="6B4F29C8FE151A97" modified="2002-06-01" version="1">MVRRTSEEISERMEDYLEALYLLSRRGSGRHVRISELSEYLEVSKPTALEMIRKLADRGLVEYERGLVKLTEKGREIGKEVWDRHREIASFLRFLGVDPKIAERDACAIEHSLHPQSFRRLRKLFHLLKEATGEPTVREILDKVRGEEGDSDGVRHRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW8</accession>
    <name>HIS6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber evidence="1">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ImGP synthase subunit HisF</fullName>
            <shortName evidence="1">IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisF</name>
        <name type="ordered locus">MK0173</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYW8"/>
    <dbReference type="SMR" id="Q8TYW8"/>
    <dbReference type="STRING" id="190192.MK0173"/>
    <dbReference type="EnsemblBacteria" id="AAM01390">
        <property type="protein sequence ID" value="AAM01390"/>
        <property type="gene ID" value="MK0173"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0173"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.172"/>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142280" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01013"/>
        </source>
    </evidence>
    <sequence length="272" mass="29848" checksum="CA7F9018E3A1A0CC" modified="2002-06-01" version="1">MALAKRIIPCLDVKDGRVVKGVRFRGLRDAGDPAELAHHYYRHGADEIVFLDISASPEGRRLMVDVVRRTAEKVFIPMTVGGGISDVEDFRRALTAGADKVSVNTAAVENPELISEAADIFGSQCVVVAIDAKREPLKPEHEHVADHIFSNDDGEYWFRVYVRGGREPVDLDAITWAKRVEELGAGEILLTSIDADGTQEGYDIELTREVCNAVSIPVIASGGCGHPKHMVEVFKEADADAALAASIFHYGKFTIEEVKEHLAERGVRVRQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY5</accession>
    <name>Q8TYY5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted pyridoxal-phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0156"/>
    <dbReference type="EnsemblBacteria" id="AAM01373">
        <property type="protein sequence ID" value="AAM01373"/>
        <property type="gene ID" value="MK0156"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0156"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.156"/>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_2"/>
    <dbReference type="OMA" id="IVNHGMY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="394" mass="43846" checksum="C40EF775B1A6A423" modified="2002-06-01" version="1">MSKLAIEGGRPIREDPIPIAQPILGDEEARAVTEVLRSGQLAQGPRVEEFEREFAQFVGCEHCVATSSGTTALQLALESAGLGPGDLAIVPSFTFIATANAALHVGADVAFVDIDLETYCMDPRSLEEVVKLLKDRVLRPRTVAVIPVHLYGHPADMDPILEIAEEHDLIVIEDAAQAHGAEYKGRRIGSLGDAACFSFYPTKNMTTGEGGAITTDDGELAERARMLRSHGERERYDHVELGYNFRMTDIAAAIGIVQLRRLEEFNERRRENARYYLKELADLEPLIELPTEKPWAKHVYHQFTIRINVEELSCTRDEFAEALRAEGVDCAVHYPTPLHRQPVYLRRGYHATELPKSERAAETVLSIPVHPGLSEEDRQDVVEAVEKVVSAFSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ33</accession>
    <name>THIC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">MK0106</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ33"/>
    <dbReference type="SMR" id="Q8TZ33"/>
    <dbReference type="STRING" id="190192.MK0106"/>
    <dbReference type="EnsemblBacteria" id="AAM01323">
        <property type="protein sequence ID" value="AAM01323"/>
        <property type="gene ID" value="MK0106"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0106"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.105"/>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000152863" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="226"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <sequence length="429" mass="47442" checksum="6F7D9A5C31A29DD5" modified="2002-06-01" version="1">MTLMEECRRTVPDTVRKIAEEEGVRPEKLARRVAEGRVVVPAHADRRDEVRPVGIGEGLRVKINANVGTSPEVCDPDLEVEKARAAVDHGADTVMDLSTGGDLREIRRRIMKAVDVPVGTVPVYEAAVEMTRRGRAVVDMDEDDMLRAIERHMEDGVDFMTVHCAVTLDALEDVLRRGRALGIVSRGGAIVAAWMIHHDAENPLYENFDYILELAREHDVTLSLGDAMRPGSVLDANDAAQHRELVVQGELVDRCREAGVQAMVEGPGHVPLDQIPAVVRLQKRVCDGAPFYVLGPVPTDVAPGYDHIAAAIGGAIAAYHGADFLCYVTPAEHLALPDVKDVILGVIATRIAAHAADTARGMKYARRENEEMAEARWNLDWDRQFELAIDPKKPRHYREERPPQAKELCSMCGEYCAIKILKDALEERR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ61</accession>
    <name>Y078_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0285 protein MK0078</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0078</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0285 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ61"/>
    <dbReference type="SMR" id="Q8TZ61"/>
    <dbReference type="STRING" id="190192.MK0078"/>
    <dbReference type="EnsemblBacteria" id="AAM01295">
        <property type="protein sequence ID" value="AAM01295"/>
        <property type="gene ID" value="MK0078"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0078"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.79"/>
    <dbReference type="HOGENOM" id="CLU_846254_0_0_2"/>
    <dbReference type="OMA" id="CYSMGDG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01087">
        <property type="entry name" value="UPF0285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009927">
        <property type="entry name" value="DUF1464"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016735">
        <property type="entry name" value="UPF0285_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07318">
        <property type="entry name" value="DUF1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018783">
        <property type="entry name" value="UCP018783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03281">
        <property type="entry name" value="methan_mark_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151263" description="UPF0285 protein MK0078">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01087"/>
        </source>
    </evidence>
    <sequence length="332" mass="35059" checksum="40F5DB080EA73F15" modified="2002-06-01" version="1">MTRMYVGIDHGTSGIKVAAYDGEGDPEFLGKAPRRKVAERGLLRSLPDEARRAVEEAECICLNYGMGDALTEFTPLEEAEDLGVGYGLRDTSGAGREFGAGRRMVEELSELGVEAYLAPGIHRDLPRLDGAFRVFSHVASGEKLGTARLALELSSSKDIVVCDTSSNTVSVVVKDGEVIGGIDACLGAPGVLQGPLDLEAIRRIDAGELSANGAFSTGGIVKIVNCAGEDPESAVEEFIQRCGKEEKEWLARLVAWEVAGLGVVYDCDEAWIGGTLSGDDEFMGVLERVLSKAFNKVAGLPPESASMGLALIAADIASGARSVLGVRISRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA0</accession>
    <name>Q8TZA0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small, Ras-like GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Era GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0036"/>
    <dbReference type="EnsemblBacteria" id="AAM01253">
        <property type="protein sequence ID" value="AAM01253"/>
        <property type="gene ID" value="MK0036"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0036"/>
    <dbReference type="HOGENOM" id="CLU_1472124_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005662">
        <property type="entry name" value="GTP-bd_Era"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42698">
        <property type="entry name" value="PTHR42698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="2">
        <location>
            <begin position="18"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="22132" checksum="7E1E6D3B8ADB3AC1" modified="2002-06-01" version="1">MKETSVKEVPELDSRAFRIAVLGPENAGKSTLVNALMGREVTEVSEVPGTTKTVSGYRWTSREFPLYVFDTVGLADERGKRSKRGVRAEDVAEKLGRYDLALFVVDVTRHVGPETLRALHVIKYAADLETLLVANKIDLLDRGELEERLERIRERTGHRPIPVSALTGEGIGRLLREIERRVREKRRTLRAAPGYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA5</accession>
    <name>RL18_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18</name>
        <name type="synonym">rplR</name>
        <name type="ordered locus">MK0030</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZA5"/>
    <dbReference type="SMR" id="Q8TZA5"/>
    <dbReference type="STRING" id="190192.MK0030"/>
    <dbReference type="PRIDE" id="Q8TZA5"/>
    <dbReference type="EnsemblBacteria" id="AAM01247">
        <property type="protein sequence ID" value="AAM01247"/>
        <property type="gene ID" value="MK0030"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0030"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.30"/>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="OMA" id="MAHGPRY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_L18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_L5_euk/L18_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="PTHR23410"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131406" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="202" mass="23020" checksum="0092C793B3562A51" modified="2002-06-01" version="1">MIALATGPRYRVPFRRRREGKTNYYKRRELIKADAPRLVARKTLNHNIAQIVDFAPHGDVTLASAHSIELRNKFGWKGHCGNTPAAYLTGYLCGLRALERGIERAVIDIGLHRPVRGSRVFAMLKGALDAGMDIPHGEEVLPPEDRIRGEHIANLARQIKEEDPEEYERRFSKYLERGLKPEELPEHFEEVKSKIEEEFGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB1</accession>
    <name>Q8TZB1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK0024</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01241">
        <property type="protein sequence ID" value="AAM01241"/>
        <property type="gene ID" value="MK0024"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0024"/>
    <dbReference type="HOGENOM" id="CLU_1507389_0_0_2"/>
    <dbReference type="OMA" id="LGWYILC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="PTHR42198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="56"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="178" mass="20354" checksum="C6AC5F03A5A72F84" modified="2002-06-01" version="1">MHGLTEGLARHFYPLVDPFIHYFGPALGMLVAAALVTLFIDIVYELVIGREELERVRKMADETKKYQEELQRAKVLGDVKRMKEIEEKMRDHSREFLKVQSQLMSKQIKAMIITFPPIIIIIYTLEYRLAHWTVKLPFYVPGVGDTLGPVGWYILCAVTVSFVLKPLAETAVKRFGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC3</accession>
    <name>Q8TZC3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tip49</name>
        <name evidence="8" type="ordered locus">MK0007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="interaction">
        <interactant intactId="EBI-16095901">
            <id>Q8TZC3</id>
        </interactant>
        <interactant intactId="EBI-16095901">
            <id>Q8TZC3</id>
            <label>tip49</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="DIP" id="DIP-60805N"/>
    <dbReference type="STRING" id="190192.MK0007"/>
    <dbReference type="EnsemblBacteria" id="AAM01224">
        <property type="protein sequence ID" value="AAM01224"/>
        <property type="gene ID" value="MK0007"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0007"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.7"/>
    <dbReference type="HOGENOM" id="CLU_028311_1_1_2"/>
    <dbReference type="OMA" id="YDMGAKM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="PTHR11093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="7">
        <location>
            <begin position="64"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="455" mass="50707" checksum="326BE4DBDFE45142" modified="2002-06-01" version="1">MVEIKEIGEVSTEETSPGAHSHITGLGLDENLKAKPVGDGLVGQEEAREAAGIVVEMVKQGRRAGHGLLLVGPPGTGKTAIAYGIARELGEDVPFVSISGSEIYGTNLSKTEFLQQAIRRAIGVEFTETREVIEGKVESLEIERAKHPLSPYMEVPSGAIIELKTQDDHRRFKVPEEIAIQLVQAGVREGDVIQIDVESGHVTKLGRAKDALEEEEEELLGVHAVELPEGPVQKKKEIKRVVTLHDLDMANVRAGRLLGFREEEITDEIRQKVDERVQKMVDEGEASLVPGVLFIDEAHMLDIEAFAFLNRSLEEEIAPILVMATNRAMAKVRGTDEEAPHGIPGDLLDRMLIARTRPFERHEIHEIIGIRARVQDIQLTDEAHEYLTDLGEEKSIRYATRLLEPARIVAEKEGSEVVEKKHVERVEEVFTDVSDSVEYMERMRRELPVMKYLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC7</accession>
    <name>Q8TZC7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0003</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0003"/>
    <dbReference type="EnsemblBacteria" id="AAM01220">
        <property type="protein sequence ID" value="AAM01220"/>
        <property type="gene ID" value="MK0003"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0003"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.3"/>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="OMA" id="DPSEYCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="175"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="352" mass="40168" checksum="4F3EA81C6566C17E" modified="2002-06-01" version="1">MSGNPFRKMPEVPDPEELIDVAFRRAERAAEGTRKSFYGTRTPPEVRARSIEIARVNTACQLVQDRLWEIVRKTPNLDELHPFYRELADALAGIDRLKSSLADVHTVAKIARLIREEYTRKIKRARDPRTAAELRRQAFGRLASTIRRKVGDALRFLRKVQPKLVDLPAIDTEMFTVTLAGFPNVGKTTLMTVLTGSRPEIAPYPFTTKGIQVGYMERPYPVQMLDTPGLLERPEEERNPVERQAIAALKHVTDAVLFLIDPTGTCGYPVEEQLELLDRVRKEFDVPVYVVLTKADLRDLWEEPDLEGEPVYKVSATERTGLKELRELLNDLARGHYSGRDRGHDEGRDEER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P58836</accession>
    <name>HIS3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="1">PRA-CH</shortName>
            <ecNumber evidence="1">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisI</name>
        <name type="synonym">hisI_2</name>
        <name type="ordered locus">MK1537</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58836"/>
    <dbReference type="SMR" id="P58836"/>
    <dbReference type="STRING" id="190192.MK1537"/>
    <dbReference type="EnsemblBacteria" id="AAM02750">
        <property type="protein sequence ID" value="AAM02750"/>
        <property type="gene ID" value="MK1537"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1537"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1698"/>
    <dbReference type="HOGENOM" id="CLU_048577_5_0_2"/>
    <dbReference type="OMA" id="TGYRSCF"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000136508" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <sequence length="141" mass="16089" checksum="14E92D9A4DFDAFB7" modified="2002-05-27" version="1">MSHVPLDPETARKVTSSLNYRFEIGGEPLVIAIAQDAENGDVLMTAFANEEAVYRTLTTGYAHYWSTSRREVWKKGEESGHVQRVVEVRVDCDKDAVLYVVEQEGGACHTGYRSCFYRRVTRDGSFEVVMDRVFNPDEVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TH01</accession>
    <name>Q8TH01_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted membrane serine protease of the Rhomboid superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">glpG</name>
        <name evidence="7" type="ordered locus">MK0052</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0052"/>
    <dbReference type="EnsemblBacteria" id="AAM01269">
        <property type="protein sequence ID" value="AAM01269"/>
        <property type="gene ID" value="MK0052"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0052"/>
    <dbReference type="HOGENOM" id="CLU_055068_3_1_2"/>
    <dbReference type="OMA" id="PQIGPYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="SSF144091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Rhomboid" evidence="6">
        <location>
            <begin position="47"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="210" mass="22654" checksum="75242EE2AC25F363" modified="2002-06-01" version="1">MSLTMLMFLLNVLAYVLSVGPDGIIRPDVLYSYGLYLHNITVHPECLITYMFLHANLIHLLFNMLGLLTFGVQLERVLSTSEFLVLYLLSGLMGGLAQTALTPDVPVVGASAAIFGLLGCLTMLRPMSMMMFLFIPMPLALFAVLYAALALFVIQSGVVTQVAHAGHLVGMIVGGVLALLYRPSEALKGLLAVAAITALLLAGYWFLTHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR3</accession>
    <name>Q8TUR3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1692</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1692"/>
    <dbReference type="EnsemblBacteria" id="AAM02904">
        <property type="protein sequence ID" value="AAM02904"/>
        <property type="gene ID" value="MK1692"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1692"/>
    <dbReference type="HOGENOM" id="CLU_064237_0_0_2"/>
    <dbReference type="OMA" id="PYRMYEH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012122">
        <property type="entry name" value="ATPase_PP-loop_MJ1016"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036670">
        <property type="entry name" value="ATPase_UCP036670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiI" evidence="3">
        <location>
            <begin position="12"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="264" mass="30338" checksum="101B1840851D5103" modified="2002-06-01" version="1">MELLRKKVSGKKICVLISGGMDSAVATKILQLSNTDVRGLHITHRWMWFTPEIEIKRISKMLGIKIDVVDITDELRRRLRGAKGKSVCKICKKIMLEIAVSKASVVATGECGMDTIAGAVLDVSRRTGIEPEFVQLPKRYFNGDDRIIVRPLIRIHESDVKRLARLLGVKVRRVGETGDLRRGRREGCPLQHLDPWVDVTDELMDEVWDVNVEALLVARRLGRRVSVKWPSFRIILEGSPEERRHVAECVWYRWVRAGRPRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR9</accession>
    <name>Q8TUR9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1685</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02898">
        <property type="protein sequence ID" value="AAM02898"/>
        <property type="gene ID" value="MK1685"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1685"/>
    <dbReference type="HOGENOM" id="CLU_1006896_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="276" mass="29526" checksum="4E6FDE9D95E0C8DD" modified="2002-06-01" version="1">MIKRILPCTVLAMIVCTAVGLQPAHSEKAPVPKPPKLEEKKEEEKKEEKERKESTLKPGKYKVAEADFKNGVVYLKPLYKPKPVIPVKVPPRVIRKLRPGTVVEVHQRGSSTVIRTASGTAEGSTVITRTESSSGTMGHTTSSSEALQTGSTSSSTSTPSHTSVGTSAGAGTYGNTTVNAKEKTVSSEGGKTAGSSKSKESKGKREKKEKNRSSKEKIKVKRPKSSGSSDLELQREASSGSSSGWAPYLVAIALVGAATGGMWLVKQRRSTTWEWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU8</accession>
    <name>Q8TUU8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Permease subunit of a ABC-type transport system involved in lipoprotein release</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK1655</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02868">
        <property type="protein sequence ID" value="AAM02868"/>
        <property type="gene ID" value="MK1655"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1655"/>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="OMA" id="GSLYRIR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="8">
        <location>
            <begin position="18"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="FtsX" evidence="7">
        <location>
            <begin position="269"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="370" mass="39412" checksum="B07662EA1E5A644E" modified="2002-06-01" version="1">MITIKVAIRNIARRSVRSLLTILGVAIGVAAVVGISAIAKGLQHDVKVAISGSASLIVKSKAAPDPLSSVLDESIEDEVRKIPGVKRTVAVWFNNRMITVGRQRQPILIAAVENRGYELVVGSKIEPIEGHLPKHGEMAFGSLIWRKLRPKVGETVDLSGQKLRVSGAFKTSSQLANMCAITTLDSVNKIRGKSISCVFVQTSDPKRVRKEIESRINGVEAITTSKAVQGMLNNLRTVELAAIALTGIAGVVGALGVANTMLMSVIERKREIGVMKAIGATNRDVMKLFLLESIILSLAGGIIGCVLGMLGSQLLVHILSYIKHQTVSVLITPEVLGLGLALALAIGVVSGLYPAWKAAKVDPVEALRYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV3</accession>
    <name>DP2L_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase II large subunit</fullName>
            <shortName evidence="2">Pol II</shortName>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="2">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">polC</name>
        <name type="ordered locus">MK1650</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="2"/>
    <dbReference type="EC" id="3.1.11.1" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUV3"/>
    <dbReference type="SMR" id="Q8TUV3"/>
    <dbReference type="STRING" id="190192.MK1650"/>
    <dbReference type="EnsemblBacteria" id="AAM02863">
        <property type="protein sequence ID" value="AAM02863"/>
        <property type="gene ID" value="MK1650"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1650"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1813"/>
    <dbReference type="HOGENOM" id="CLU_001154_0_0_2"/>
    <dbReference type="OMA" id="KRRNCDG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="PTHR42210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000152576" description="DNA polymerase II large subunit">
        <location>
            <begin position="1"/>
            <end position="1240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <sequence length="1240" mass="140145" checksum="4BEEE6117BCDD9AD" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV7</accession>
    <name>PUS10_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="1">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pus10</name>
        <name type="ordered locus">MK1646</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUV7"/>
    <dbReference type="SMR" id="Q8TUV7"/>
    <dbReference type="STRING" id="190192.MK1646"/>
    <dbReference type="PRIDE" id="Q8TUV7"/>
    <dbReference type="EnsemblBacteria" id="AAM02859">
        <property type="protein sequence ID" value="AAM02859"/>
        <property type="gene ID" value="MK1646"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1646"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1809"/>
    <dbReference type="HOGENOM" id="CLU_028780_2_0_2"/>
    <dbReference type="OMA" id="DGGLYIK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="PTHR21568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000407389" description="tRNA pseudouridine synthase Pus10">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <sequence length="436" mass="50019" checksum="10983DC963276DB4" modified="2002-06-01" version="1">MIDRLREALKRFNPCDSCLGRAFGYGLTGLENRERGRAIKLYLGMRAHLEGEEETLELLARSGLEEAAAVLDDPPEPEPCGVCRGVLDKVDEFAEVVACELKDLEFRGFVVGSRWPEEIRKAEKELWETLGVEGEPIKREFNREVGKRVEHLLDVRADPRNPDIEVVFDFRPSLEDPKFEVHVRPIYVRGRYLKLRRGIPQTKWPCPRCRGAGCPNCDFTGKLYTESVEELIGMVLKDAFLAESHKFHAAGREDIDVRMLGNGRPFVMELLYPKRRNVDLKEIEGEINRKVGDDVQVVGLEYGDPEDVGKVKDLSERSRKRYRAWVKFGKPVPEDKLREVLKGLERSVIEQRTPRRVLHRRADKVRRKRVHEAKLIEYDGDRAVIEFLCDPGLYVKELISGDAGRTRPSLAELVEVEAECERLDVIEFLDEGGDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX6</accession>
    <name>Q8TUX6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein implicated in protein export</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1626</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1626"/>
    <dbReference type="EnsemblBacteria" id="AAM02839">
        <property type="protein sequence ID" value="AAM02839"/>
        <property type="gene ID" value="MK1626"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1626"/>
    <dbReference type="HOGENOM" id="CLU_022017_5_0_2"/>
    <dbReference type="OMA" id="FIIIDSG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Polysacc_synt_C" evidence="7">
        <location>
            <begin position="344"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="519" mass="55448" checksum="0662870E3CCCF5F6" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ4</accession>
    <name>DAPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">MK1607</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUZ4"/>
    <dbReference type="SMR" id="Q8TUZ4"/>
    <dbReference type="STRING" id="190192.MK1607"/>
    <dbReference type="EnsemblBacteria" id="AAM02820">
        <property type="protein sequence ID" value="AAM02820"/>
        <property type="gene ID" value="MK1607"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1607"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1770"/>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_2"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000103198" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="300" mass="32345" checksum="42EFA59F6E0D8883" modified="2002-06-01" version="1">MGFQIEGVIPALITPFTDDLKGINEEGLRENVSRLLEAGVHGVVPAGTTGESSTLSHAEHRRVIEIVVDEVNGKVPVIAGAGSNSTREALELSTYAEDVGADAILSVVPYYNKPPQEGLFIHFSKIAEAVECPIILYNVPSRTGCALEPETAAKLAEEYSHIVGVKEASGDLDVVQRFIEETPDDFILLSGVDELTLPILAVGGVGVISVTANVAPELMVEMYEAWKSGDVERARELHYELLPLHRALFTETNPIPVKAAVELVGMASSPPRPPLKEAREDTKELLRRELKKLGLLPEGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-12-20" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV08</accession>
    <name>RS15_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps15</name>
        <name type="synonym">rpsO</name>
        <name type="ordered locus">MK1593</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV08"/>
    <dbReference type="SMR" id="Q8TV08"/>
    <dbReference type="STRING" id="190192.MK1593"/>
    <dbReference type="EnsemblBacteria" id="AAM02806">
        <property type="protein sequence ID" value="AAM02806"/>
        <property type="gene ID" value="MK1593"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1593"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1754"/>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="OMA" id="EEHPKDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_S15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_S13/S15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_S15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="PTHR11885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000115608" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="149" mass="17299" checksum="04BC022C5731AA51" modified="2002-06-01" version="1">MARMHSRDRGKSGSTRPPRVAPPSWVEYSPEEVESLVVDLAKQGYEPAMIGIKLRDEYGIPDVKLITGKKITEILEEHGLAPELPEDLLNLIRRAKRVREHLKRHPKDLHSKRGLQLIESKIHRLVKYYKRKGVLPEDWKYDPEALHVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV55</accession>
    <name>RS8E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps8e</name>
        <name type="ordered locus">MK1545</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV55"/>
    <dbReference type="SMR" id="Q8TV55"/>
    <dbReference type="STRING" id="190192.MK1545"/>
    <dbReference type="EnsemblBacteria" id="AAM02758">
        <property type="protein sequence ID" value="AAM02758"/>
        <property type="gene ID" value="MK1545"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1545"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1706"/>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="OMA" id="NPNYVRR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_S8e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_S8e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_S8e/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_S8e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="PTHR10394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000122268" description="30S ribosomal protein S8e">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="133" mass="14876" checksum="2B5470E3FDB87DC0" modified="2002-06-01" version="1">MGVWHGRSLRKPTGGRIRPHRKKRKFEMGNPPTETLVGEERKLKERRGMGGNVKKGLKFATHANVADPETGEVKCVRIEEVVKNPASQYYERHGVITKGAIIRTEIGLAKVTNRPGQEPVVNAVLIKEEEEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV64</accession>
    <name>Q8TV64_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1534"/>
    <dbReference type="EnsemblBacteria" id="AAM02747">
        <property type="protein sequence ID" value="AAM02747"/>
        <property type="gene ID" value="MK1534"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1534"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1695"/>
    <dbReference type="HOGENOM" id="CLU_244882_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1729"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1693"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1698"/>
            <end position="1712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1771" mass="199475" checksum="25B59B931C6E6A76" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV70</accession>
    <name>Q8TV70_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02741">
        <property type="protein sequence ID" value="AAM02741"/>
        <property type="gene ID" value="MK1528"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1528"/>
    <dbReference type="HOGENOM" id="CLU_2340193_0_0_2"/>
    <dbReference type="OMA" id="VGEIGEC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="11586" checksum="A6AA308DFEAE499D" modified="2002-06-01" version="1">MELRCPYCGSRSCVNESALSLFLRASERAARYVVKGRDDVDEDPNFRPSFGEVGICKETSKRIWVCPYCHSLIPELEPDGMTARCPECGREANVAPSRRKIVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA4</accession>
    <name>Q8TVA4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase, related to NifB/MoaA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1488</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02701">
        <property type="protein sequence ID" value="AAM02701"/>
        <property type="gene ID" value="MK1488"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1488"/>
    <dbReference type="HOGENOM" id="CLU_736956_0_0_2"/>
    <dbReference type="OMA" id="MYELTED"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007549">
        <property type="entry name" value="DUF512"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014494">
        <property type="entry name" value="Fe-S_OxRdtase_NifB-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045375">
        <property type="entry name" value="Put_radical_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04459">
        <property type="entry name" value="DUF512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19238">
        <property type="entry name" value="Radical_SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018654">
        <property type="entry name" value="Fe-S_or_NB_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elp3" evidence="1">
        <location>
            <begin position="6"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="349" mass="38747" checksum="D35F7C37E020F122" modified="2002-06-01" version="1">MEEPENLAVVPPSCEAGPCGNSCVFCYIRQNPPEVLREHGGNVDTTRHDTLNDPHLEERVERARDRYPDLRLRIVDTAGNVGIDEGRVESLAAAGIDEVQISLHTTVPETRKKLMGNPNADELLELLPKFEDVGIDVIADLVLTPGYNLRELPRTCEDLETMGARQVRVFPVGGTDLARGFRFPTRRELEWMRETSRRLDCELNIEVIPSPTIDALLGEPAFDPPDLPEPDFEAVIVVGELAAPIFEPAVRELENVELLVVKNRVFGGVIGASSLLTAKDVLREIERYEPRTEFPVLILPDAMFSPDGRTLDGWSREELVGKLAALGYTVVTCRKPEEVAKVLASPSPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-12-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA7</accession>
    <name>MTRF_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydromethanopterin S-methyltransferase subunit F</fullName>
            <ecNumber evidence="1">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtrF</name>
        <name type="ordered locus">MK1485</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Unlike other orthologs, MtrF from M.kandleri is not located in the mtr operon.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVA7"/>
    <dbReference type="SMR" id="Q8TVA7"/>
    <dbReference type="EnsemblBacteria" id="AAM02698">
        <property type="protein sequence ID" value="AAM02698"/>
        <property type="gene ID" value="MK1485"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1485"/>
    <dbReference type="HOGENOM" id="CLU_2662350_0_0_2"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01099">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011307">
        <property type="entry name" value="MeTrfase_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013347">
        <property type="entry name" value="MeTrfase_F_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09472">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006523">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02507">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147549" description="Tetrahydromethanopterin S-methyltransferase subunit F">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01099"/>
        </source>
    </evidence>
    <sequence length="75" mass="8130" checksum="0186C402CCFCEF28" modified="2002-06-01" version="1">MAEEGSELKEVIIGAPAMADTDRADTYVNDVRDSSQFFGRDARLYFGLNVNRFAGLACGMVFAGVLLVPLLLLAF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-27" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC1</accession>
    <name>RS13_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps13</name>
        <name type="synonym">rpsM</name>
        <name type="ordered locus">MK1471</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVC1"/>
    <dbReference type="SMR" id="Q8TVC1"/>
    <dbReference type="STRING" id="190192.MK1471"/>
    <dbReference type="EnsemblBacteria" id="AAM02684">
        <property type="protein sequence ID" value="AAM02684"/>
        <property type="gene ID" value="MK1471"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1471"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1627"/>
    <dbReference type="HOGENOM" id="CLU_103849_0_0_2"/>
    <dbReference type="OMA" id="IRAYRGI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_S13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132182" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="136"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="151" mass="17340" checksum="4E3B17FD2B987D7C" modified="2002-06-01" version="1">MADDEVKPIVRIADVDLDGHKKVPYALTGIKGIGIRMAYAICRELGLDEEKKLGELSDEEIERIEEEIKKLSEGESNIPSWMYNRQKDYETGEDMHLVGAELEMTVKQDIDRLKKIRAYRGIRHELGLPVRGQRTKSSFRRGRTVGVKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH0</accession>
    <name>Q8TVH0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02632">
        <property type="protein sequence ID" value="AAM02632"/>
        <property type="gene ID" value="MK1419"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1419"/>
    <dbReference type="HOGENOM" id="CLU_2010115_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="123" mass="13530" checksum="A5095FD72D1A0AA8" modified="2002-06-01" version="1">MEKVVLEFGCEFGARLWVIQQVICILVSSLVIPILAHHTVRAVRRHDLDCALASFGMLLLILAAGVITPLNTLLHLYSPPRIKWGIILWTYIGSLGAGMVTFGMGMFKVLTRRPAAPFGGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVJ3</accession>
    <name>Q8TVJ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02609">
        <property type="protein sequence ID" value="AAM02609"/>
        <property type="gene ID" value="MK1396"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1396"/>
    <dbReference type="HOGENOM" id="CLU_1412373_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="20800" checksum="3542FB28505F4D5F" modified="2002-06-01" version="1">MIRYIVTVLVVASLLAAPVAAQEGGTTTGTGQATPAVQPSQPAPGGTTTATGGGGHAGVSQPQPVSTAGKAQKVESGKKAGEAKKKKEKRRKKPEKKRTRKAGLQGLTRSLRELRYYALATAVGLAIMAVMMGYGLVKFERRLSQRSMKAKARKAKKTRKPPKVERKLRKTEPKEEEVERVRKLWKQVKEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL3</accession>
    <name>Q8TVL3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-3B methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cobJ</name>
        <name evidence="6" type="ordered locus">MK1376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1376"/>
    <dbReference type="EnsemblBacteria" id="AAM02589">
        <property type="protein sequence ID" value="AAM02589"/>
        <property type="gene ID" value="MK1376"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1376"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1531"/>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="OMA" id="VWDDRMV"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="5">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="254" mass="28020" checksum="6A18AD6C75ECB206" modified="2002-06-01" version="1">MIVGKLYVVGTGPGDPDLMTVKAQRVLRHVDVVVGYRTYVDLIEEILPEDVEIKRYGMREELDRAREAVRLAADGFEVALVSGGDPGVYGMAGVVLPIAVEEGVEVEVVPGVTAACAASALLGAPLMLDFAAVSLSDHLVPLEEILERVRAALEADFVLVVYNPNSSERRHIFEAFVDVLEEIVEEDRPVGIVRNAYRERQSVEVVRVRELRDLADRIDMRSILIVGSSRTRMVGDWLVTERGYSSRTGRNSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL7</accession>
    <name>Q8TVL7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small, Ras-like GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1372"/>
    <dbReference type="EnsemblBacteria" id="AAM02585">
        <property type="protein sequence ID" value="AAM02585"/>
        <property type="gene ID" value="MK1372"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1372"/>
    <dbReference type="HOGENOM" id="CLU_040729_9_3_2"/>
    <dbReference type="OMA" id="DILEFGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="187" mass="20460" checksum="89BF115B3FC1ACD3" modified="2002-06-01" version="1">MISEDSGSNEDREVKIAVIGPEDAGKTTVVRQLSDKFTTVSPRGKTVGIDFGKCKYYEGVYMFGVPGHLRFKFVMRLGARNADGMILVIDSADPRIDKAVKIYNIVKSVVKNPHRVVVFANKQDLPDALSPEQVGELVKRALGISPPVIGTVAIKGEGLREGLDTLLFQPTYNGTNEIDDIEIKGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ3</accession>
    <name>Q8TVQ3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02548">
        <property type="protein sequence ID" value="AAM02548"/>
        <property type="gene ID" value="MK1335"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1335"/>
    <dbReference type="HOGENOM" id="CLU_1472099_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="183" mass="19903" checksum="D49915CFAFA689F6" modified="2002-06-01" version="1">MTPWPLPREIEEIVRELPPGAYVASARVEGVPEYDLLSDALRDALGRSSTSPYRVLVDRYLEEVDAPLVFQASNHFQLNHHARRALERLAGSVPVLVLNRGYLPAVECEDLTDELAGTDSAEVEAVFDDGRTVTLVEDPSRLLRALAVAGIGLLILRTTVDESVDFWALTALGLAGLAVSKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS4</accession>
    <name>Q8TVS4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted DNA-dependent DNA polymerase, component of a thermophile-specific DNA repair system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02527">
        <property type="protein sequence ID" value="AAM02527"/>
        <property type="gene ID" value="MK1314"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1314"/>
    <dbReference type="HOGENOM" id="CLU_017487_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="669"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="913" mass="100873" checksum="EEEE8FBE31C774FF" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU7</accession>
    <name>Q8TVU7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02504">
        <property type="protein sequence ID" value="AAM02504"/>
        <property type="gene ID" value="MK1291"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1291"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="214" mass="25302" checksum="3812F54C345A0412" modified="2002-06-01" version="1">MVRSHVHIPIPSDPVERIRVLRVLREVHRRGRKPSLEVTYRTVNGSTCGPYYVARWRRGSRHRHGRTLYLGKPENESVSFVEWLVSLDKKQVLELARHLMRNLRSVLKTLLTEVSSLPYKRARRVLTRGLALTFDARPSESPRIRDLLEELPDRLESFTVRTLGGWPAHYSSYLRKVIRSRRKSLNGRHEVPDVQLELERWKLQHGWRLRPRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX3</accession>
    <name>Q8TVX3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1265</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02478">
        <property type="protein sequence ID" value="AAM02478"/>
        <property type="gene ID" value="MK1265"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1265"/>
    <dbReference type="HOGENOM" id="CLU_1140571_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="243" mass="27505" checksum="77866D61B22C8484" modified="2002-06-01" version="1">MHPTRPFHGKFLRVLPEGEGPELSIAFSWNLSRTAADAGSLESVLERREERGETFEEFVGRLREDGWLVGRLRREGGKSRVVLEDVRKFRFPCSGPREVRVRGRVGVLSGTVSVTELVLDDGSREVTLVLDRGLRESFEDVESVVTEVVEGEFRRVVQDGELESDVVRYGIVRAVRDVVEELVERGEAEYEVAVRAEETVNGDEAGVLADEWRFPNAKAALRSLVREILRTRLREVLEELVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX5</accession>
    <name>Q8TVX5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-29 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02476">
        <property type="protein sequence ID" value="AAM02476"/>
        <property type="gene ID" value="MK1263"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1263"/>
    <dbReference type="HOGENOM" id="CLU_1237928_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="223" mass="24755" checksum="D6CD5F8B9D7C8835" modified="2002-06-01" version="1">MFELAVKAFGLPDSTVTHAKRGLQIMDDRASRGRKPVTRGDYGKVTRGVARLVRHAHTTLVVPGLDVVTKWAKRYGKRPWKIVRNVLEDGGGLLDETPLRFEDLPPGAAPIAAVVRTGNARKVVEEAFLTEYPLFAAMMCAVLEDAARTMLERFSEEEVETYGEFCRKHPGRHGSSFLIGVALWGWLEERTGETIDVDETLRSIEKLARDPVEEAVNGILDVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX6</accession>
    <name>Q8TVX6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase involved in chromosome partitioning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02475">
        <property type="protein sequence ID" value="AAM02475"/>
        <property type="gene ID" value="MK1262"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1262"/>
    <dbReference type="HOGENOM" id="CLU_864987_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="303" mass="34453" checksum="417947FD0AC064F8" modified="2002-06-01" version="1">MAKVSKVFVVGFTARKGGTGKTTCSFNVGFEFARRRKWKGVKSHPVFFVDVDPQAIGKESERAATTLTDLFRIGPFDLTPLKLEGDRMVEARRVTKETVERTGGFELFHVKYSPDPEEFDNADPMCSVHDKVFLAHLSTHISESPGMMDFGKFLNAITGAGFDGIVVLDTPPLNVRTTFVLNALEHCDLVVPVLNPESYDQIPDFIRRHPVRFIVLNQMLKGRTKEAEKIRREIEGYLKRTTLDEDDVIWIPSNDQLRSCTNTGVPARCRNPKPQAVTPKFHHIAWRIEVERERRSKISEIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX9</accession>
    <name>Q8TVX9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted DNA methylase containing a Zn-ribbon module</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="REBASE" id="5983">
        <property type="entry name" value="M.MkaORF1259P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02472">
        <property type="protein sequence ID" value="AAM02472"/>
        <property type="gene ID" value="MK1259"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1259"/>
    <dbReference type="HOGENOM" id="CLU_007795_2_0_2"/>
    <dbReference type="OMA" id="TVIAWIW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044260">
        <property type="term" value="P:cellular macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043412">
        <property type="term" value="P:macromolecule modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044238">
        <property type="term" value="P:primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009537">
        <property type="entry name" value="DUF1156"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014455">
        <property type="entry name" value="N6_adenine_Mtase_MK1259"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06634">
        <property type="entry name" value="DUF1156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009427">
        <property type="entry name" value="UCP009427_DNAmts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DUF1156" evidence="1">
        <location>
            <begin position="12"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="948" mass="109261" checksum="289789CC397BC4D7" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVZ1</accession>
    <name>Q8TVZ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferrous ion uptake system subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">feoA</name>
        <name evidence="3" type="ordered locus">MK1247</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02460">
        <property type="protein sequence ID" value="AAM02460"/>
        <property type="gene ID" value="MK1247"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1247"/>
    <dbReference type="HOGENOM" id="CLU_150646_6_3_2"/>
    <dbReference type="OMA" id="KISAMSF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="8012" checksum="EBA6ECE7803F4125" modified="2002-06-01" version="1">MGGAVKTLLEVPEGRTVRVVDVAAGKGAAAMLYELGIRPGARVEVVKSGPGPVIVRVGGAKYSLGRGLAAKVIVEEEQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW26</accession>
    <name>CRCB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB</name>
        <name type="ordered locus">MK1211</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW26"/>
    <dbReference type="SMR" id="Q8TW26"/>
    <dbReference type="STRING" id="190192.MK1211"/>
    <dbReference type="EnsemblBacteria" id="AAM02424">
        <property type="protein sequence ID" value="AAM02424"/>
        <property type="gene ID" value="MK1211"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1211"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1313"/>
    <dbReference type="HOGENOM" id="CLU_114342_3_2_2"/>
    <dbReference type="OMA" id="FLMFESF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110229" description="Putative fluoride ion transporter CrcB">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="131" mass="13434" checksum="B670A1CBBF05479C" modified="2002-06-01" version="1">MVKIGARELAAVAIGGALGAVCRYLLSGLVPQVRGFPMGTVLVNVLGSFVLGFLTWSTMLGLRLSPEVRALATVGFCGGLTTLSTMAYETVELLKASPVLSILYLTANVVLGIAAVLGGMAAAHVVWSGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW27</accession>
    <name>Q8TW27_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02423">
        <property type="protein sequence ID" value="AAM02423"/>
        <property type="gene ID" value="MK1210"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1210"/>
    <dbReference type="HOGENOM" id="CLU_146749_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="DUF190"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF190" evidence="2">
        <location>
            <begin position="17"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="117" mass="13163" checksum="A551007B9C417E76" modified="2002-06-01" version="1">MVGEGVRLVRKIEEPVKLKVYLLEGDKVDGVPAVDWILERVRELGLRGATVHRCFAGCGRRGRSEARILRTSMNLPVVVEVVDSREKVERLLELLKERLGVGVVTLERLEVAYDLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW68</accession>
    <name>Q8TW68_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02381">
        <property type="protein sequence ID" value="AAM02381"/>
        <property type="gene ID" value="MK1168"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1168"/>
    <dbReference type="HOGENOM" id="CLU_893152_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="311" mass="34316" checksum="339B20C0629811F8" modified="2002-06-01" version="1">MRVGIAVLAALLATLGAATAAEYPIKGEVTGTFEVHVEADPGTQVDTNEYAVVVGSKYVYLIMVEEDDLQDSGKFKTQPFLVPVPADKVEVNGSLVKFPDGLDDTFAAYCLNPMGRHPRGEMVVPKWNEIEDRFVEIKVVAGHGQVRTEERHLAPEWIKAVCKRVEGVVDPGEWKRDAQTIIWVTEAAVPENVQVNVENRVQIDVNATVQVNAQDFFQINESELRELANAGDEMARQLLEFFSNKEELENLVNTAQSALDFVKQHLSDIVTTAQTAKEAYETAKSVFGKIPVSPAVIIVALAALAIFRRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA8</accession>
    <name>Q8TWA8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1128"/>
    <dbReference type="EnsemblBacteria" id="AAM02341">
        <property type="protein sequence ID" value="AAM02341"/>
        <property type="gene ID" value="MK1128"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1128"/>
    <dbReference type="HOGENOM" id="CLU_001265_10_3_2"/>
    <dbReference type="OMA" id="CAFVGAR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="2">
        <location>
            <begin position="192"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="364" mass="37778" checksum="DF270126DBFF8164" modified="2002-06-01" version="1">MRPLIAHVVGIACAVSLTSVLPILPLYVVDAGYPRSFVGVMNLVHNVTQLLARPVGGALTDAYGPRRLAPLGALGYALSDLLVLVPHPGTLLASRVSLGLGMGALWPPLLSTVADSGRSLGVFNAVTQLPMVLAPPLGGYLYRSVGPWAFAVLALPALLAVPLAGSLPDVRTSREKVSPLEVLKRSLEVDRELFRASLPAFATAAVQPTIESFVPLLLKKLGADPAQIGLVLAIRNAVAVTLQAPCGWLADRYSPTVLAALGCVLAALGVLVLPFVESVPVAVVLLCVGGVGYSLAQPSSLKLVAEVSGERRGLGMGWWGSIMSLGRLTGSLPGLLADVSLSAVFYISGAIPLASVLPLLRRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB3</accession>
    <name>Q8TWB3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N5,N10-methylenetetrahydromethanopterin dehydrogenase (H2-forming)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hmdIII</name>
        <name evidence="3" type="ordered locus">MK1122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1122"/>
    <dbReference type="EnsemblBacteria" id="AAM02335">
        <property type="protein sequence ID" value="AAM02335"/>
        <property type="gene ID" value="MK1122"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1122"/>
    <dbReference type="HOGENOM" id="CLU_069755_0_0_2"/>
    <dbReference type="OMA" id="INAPKDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010063">
        <property type="entry name" value="HMDII/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500166">
        <property type="entry name" value="HMDII_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01724">
        <property type="entry name" value="hmd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMD" evidence="2">
        <location>
            <begin position="174"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="318" mass="35459" checksum="CF4DC4F72B888A7C" modified="2002-06-01" version="1">MEVHYELPLGGAHLAYRAAEAGHDVVLCDPSFDISEESMERLESAGVELVTDDTAGVEHGEIQILFTPFGHTVKIARELLDDVREGAVLCTTCTCPPIALYHGLEGELRTKREDVGVSSFHPAGIPGAETQDLILVADARSEESGIELASEEQVERCVNLAEDMGYDVYVLDPELISSIGDMSVVLTVRIVQAICNYFSVARICGAPIEMIEEQVEEVLSTMAYLVGRYGLDAFDRMDGKLLLRSLRNMALTEDVERVVDFFERCYVDLLSGDRESMRELSSMMVPPDKLVDRVREIVGEAPVQYARRRFFEERRWGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC5</accession>
    <name>Q8TWC5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02323">
        <property type="protein sequence ID" value="AAM02323"/>
        <property type="gene ID" value="MK1110"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1110"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1165"/>
    <dbReference type="HOGENOM" id="CLU_1159063_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="239" mass="27305" checksum="9E020F1CD5B3392B" modified="2002-06-01" version="1">MELAELAEKVAGERPELAGWAFRILVEARDRRQWGIAEVAFRNALGEVVRLRDLKNKPPTTWAKKWCKHGVFRGMEPVIARGEFREFVEQLPDDWLTTPGERARRQLLQGHFRKSAGRARYAIRIGGIMYVPTLVGPIATYGLAYYLVRSVPDHLLAGARTPMKLVEVAEENELAITSKDIERAGLKPEARTFRNLRYVTCKVRIFFPTEECKLAGRFDVGLLELVTRYRKGIMETFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD7</accession>
    <name>Q8TWD7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-35 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02311">
        <property type="protein sequence ID" value="AAM02311"/>
        <property type="gene ID" value="MK1098"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1098"/>
    <dbReference type="HOGENOM" id="CLU_2091315_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="116" mass="12833" checksum="41F6C0206B329B06" modified="2002-06-01" version="1">MAVFALWSLLRLLWGPRAGPGTVVAVVNLTAVGAATWALFSGHWERLSEALQARSTACLVFFAVTIPLTVYGAWRIIHDFRRRLCGRPDPRDVAAARVLLQGARKLWKSSEERGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH5</accession>
    <name>Q8TWH5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02272">
        <property type="protein sequence ID" value="AAM02272"/>
        <property type="gene ID" value="MK1059"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1059"/>
    <dbReference type="HOGENOM" id="CLU_1901944_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="133" mass="13917" checksum="B5051F8483393A1A" modified="2002-06-01" version="1">MQMLAERVLKWGPILVPALALIGLPGLRVILRSRVASEGEARAVVRRAGLYLGLASPCAGVAAAYTLSVGSVLLGRPELAAWTGPALILTPPVTLLLSYVPGRSESMAKAMLVSSLTTSVPTTTAVWYALSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN5</accession>
    <name>Q8TWN5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0997"/>
    <dbReference type="EnsemblBacteria" id="AAM02210">
        <property type="protein sequence ID" value="AAM02210"/>
        <property type="gene ID" value="MK0997"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0997"/>
    <dbReference type="HOGENOM" id="CLU_1943865_0_0_2"/>
    <dbReference type="OMA" id="IPWEESD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007417">
        <property type="entry name" value="DUF473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04322">
        <property type="entry name" value="DUF473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="129" mass="14083" checksum="3A4ED821C8378526" modified="2002-06-01" version="1">MKMLAITGVSFRVVEQLLTQGSTQIIVRSGNNLDAVVRLQPGDLVFVTTVGKRELKKGVSGVVATVRSVSVVHEHVVTGGDEHFEREFVAARLILNRVGSGRVREYKDCGVGRRVEVDVEMESAVSEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP9</accession>
    <name>Q8TWP9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted archaea-specific RNA-binding protein containing a C-terminal EMAP domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0983"/>
    <dbReference type="EnsemblBacteria" id="AAM02196">
        <property type="protein sequence ID" value="AAM02196"/>
        <property type="gene ID" value="MK0983"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0983"/>
    <dbReference type="HOGENOM" id="CLU_110988_0_0_2"/>
    <dbReference type="OMA" id="PRYLLAW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="106"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="23121" checksum="2C975E37A448BB25" modified="2002-06-01" version="1">MTKPVEPDDPRIRLLLAYVNRVKDRAASGGYSGVNRRQLTEVLQSIESTLQELRYSYLPPENVSERLKDLLPDLEDVVDPEEPELRWLVEYLEHADEVFEREIGDEADAVLCVVGEVGSVREHPNADNLYITVVNTGRFGKRTVVTNLTDVEEGDSMAVALLPPREFSGVVSEGMFCGEADGEPGEIIEPPERGEVRSIVLEWIEGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR5</accession>
    <name>IF2B_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif2b</name>
        <name type="ordered locus">MK0967</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWR5"/>
    <dbReference type="SMR" id="Q8TWR5"/>
    <dbReference type="STRING" id="190192.MK0967"/>
    <dbReference type="EnsemblBacteria" id="AAM02180">
        <property type="protein sequence ID" value="AAM02180"/>
        <property type="gene ID" value="MK0967"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0967"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1012"/>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="OMA" id="NVKHHKS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="PTHR23001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="SSF100966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="SSF75689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137422" description="Translation initiation factor 2 subunit beta">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <sequence length="138" mass="16267" checksum="29D53063051F0B14" modified="2002-06-01" version="1">MVEYDYEELLERAYEQLPEEVLEDRRFEMPKPKVSVEGKTTVIRNFKEISKKLDRDPEHITKYFLKELGTAGHVDGGRLILHGVYHPKLVEEELKNYVEEFVLCPECGKPDTKLVREDRQWILKCEACGAWSSVRRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS0</accession>
    <name>Q8TWS0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02175">
        <property type="protein sequence ID" value="AAM02175"/>
        <property type="gene ID" value="MK0962"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0962"/>
    <dbReference type="HOGENOM" id="CLU_2550279_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018662">
        <property type="entry name" value="DUF2095"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09868">
        <property type="entry name" value="DUF2095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="82" mass="10046" checksum="B0B6AB390E77FFBE" modified="2002-06-01" version="1">MPEDPLRGYEPSVRDYLARCDTDEEALEVLQYLVKRGELDEERASELEREIREKGVRSLVERRHFGYYLERYYDPEERRYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV2</accession>
    <name>Q8TWV2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420-reducing hydrogenase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0929"/>
    <dbReference type="EnsemblBacteria" id="AAM02142">
        <property type="protein sequence ID" value="AAM02142"/>
        <property type="gene ID" value="MK0929"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0929"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.971"/>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="OMA" id="REYGCEK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03289">
        <property type="entry name" value="frhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FrhB_FdhB_N" evidence="3">
        <location>
            <begin position="22"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="FrhB_FdhB_C" evidence="4">
        <location>
            <begin position="112"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="280"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="33625" checksum="320B6EFBB5B397F7" modified="2002-06-01" version="1">MSKLDEIMVKGDPELLGDHLGVFTARSTERKVTKVTQDGGIASAVMIYGLEEGLFDGVIAAVADPDDPEEPWKPRPVVITDPDEVLEAAGTKYTYCPNVSVLKEAVRSYGCEKVAMVGTPCQIRAVRKAQLCPIGMRHVPDKIELLIGIICMENFPYEGMKTIIEQLCGVWIREVTKMDIGNGKFWVYTKDGEVKSIPIDETHPFEGEPCHVCTDYCAELSDLTAGSVGSPDGWSTVIVRTEKAKEILDDMVEQGLLEVKSIEEVKPGLGLIQKLAQVKKNKNQKEIEERKELGLPEPGKVHESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV9</accession>
    <name>Q8TWV9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-19 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0922</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02135">
        <property type="protein sequence ID" value="AAM02135"/>
        <property type="gene ID" value="MK0922"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0922"/>
    <dbReference type="HOGENOM" id="CLU_632552_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Cas_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="433" mass="48877" checksum="FDE698550790333B" modified="2002-06-01" version="1">MPVHAVVGFGYPKYDPVSWFAERSLIETLEVKTAESRCLELREGSTLRLEARRLGRREMSATALLERVEEPGEVHVFASSSLKGQTVDGDTVEDRMGEYLVDPLNDVGFEVEVHWSSAFDPLSLLPELIDTLTRLDDRVVLDLTHGVRIQPAISAVAGMAAARLPGSDMELVPVYGAAGVEVREVLRWSDGADGSRGIILDIRRHLESAMAFNKVVDYTLDPSREHLEELCELTESALRELDVPDDRLERVRELVIDEGALRGLADALRSNYPRKVVKEARKWEPKSPEDVLDRLHIESSGRERAEKYLRALSNFLARALNPFLQGLDLPKSPWKAAVQLAEWLCDRGYYAASVLLLRELVYLRTVYEWAEEHGHGELKEVVEEYLRALIDPDARKPRGVYDELCSTIEEVTDGEAGDPDDLVRKIEREILNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW1</accession>
    <name>Q8TWW1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cpsG_2</name>
        <name evidence="10" type="ordered locus">MK0920</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0920"/>
    <dbReference type="EnsemblBacteria" id="AAM02133">
        <property type="protein sequence ID" value="AAM02133"/>
        <property type="gene ID" value="MK0920"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0920"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.961"/>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OMA" id="PHNPEPL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PGM_PMM_I" evidence="7">
        <location>
            <begin position="3"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_II" evidence="8">
        <location>
            <begin position="167"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_III" evidence="9">
        <location>
            <begin position="255"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="PGM_PMM_IV" evidence="6">
        <location>
            <begin position="393"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM02133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="456" mass="49070" checksum="0D7B814A4ED508A9" modified="2002-06-01" version="1">MGKLFGTFGVRGVVGEDLTEDVARRLGLAFGTYLGGDAEVLVGGDTRTSTDTLKDALISGLTAAGCDVVDVGIAPTPAVQYLVDAEGFDAGAVVTASHNPPEFNGIKLLGSDGCGLSREDEQEIERIYFEENPDRASWDRVGNRVSAPDLLLNFEEAVLDYVGDFDGEGLRVVVDAANGAASSVTPRLLSELGVEVISVNAHPDGRFPGREPEPSEENLETTMNMVRAAGADFGLAHDGDADRLILITGDGEFVPGDYSLAIVAAWALDEGKGSQVVTPVSSSMCVQKVVEDRGGEVIWTKVGEPVVVEELKRAEDPALGGEENGGIIYPDFHLSRNGIITALLICKLVAEVGSLDDLLAEVPKYHLHKTGVECPDDLKPKVMERVESLVEEEELEDVLTIDGVKLFYEDGWVLVRPSGTEPLIRVFGEARDRETAIRRVEHWKERVEEIVSELKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ1</accession>
    <name>Q8TWZ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0890"/>
    <dbReference type="EnsemblBacteria" id="AAM02103">
        <property type="protein sequence ID" value="AAM02103"/>
        <property type="gene ID" value="MK0890"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0890"/>
    <dbReference type="HOGENOM" id="CLU_043916_0_0_2"/>
    <dbReference type="OMA" id="FVGCAML"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="393" mass="40377" checksum="0775888A3550D5A9" modified="2002-06-01" version="1">MGRTCSGGAFLGIFSGMLSGYIPGLHPNTFFSEFDPTYLDREDVLTFASACSAVNVPLSKVESMFLGVPDDQASVAVLIPLQRYTIEGRAEEAARLVALGTLSTGFFAAVALPYIVKIVGPMYVASKPIIPWLVLTILALQTYDNGLRGLAVFSASSAVGYVVLSGPLDVASPLEAMLSGFYAIGPGLSCLMNRTSIPKQRPGKPAISGKELPKCGILAALAGCALGFLPGLGPANVVSVLTRLGVDTTERYLLVTSGIDAADAVSSIVALHALGNPRSGASVFIQRSVGDITYPEVLASVGVYLLVSVLGAWLLIFSTRVLGGVLSGARARVLTGTVVLGLLALMTFHGLGSLGTAIACAGIGIYALRSGVDPSLCTSALALPTALKLLGVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-10" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX35</accession>
    <name>RS3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3</name>
        <name type="ordered locus">MK0842</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX35"/>
    <dbReference type="SMR" id="Q8TX35"/>
    <dbReference type="STRING" id="190192.MK0842"/>
    <dbReference type="EnsemblBacteria" id="AAM02055">
        <property type="protein sequence ID" value="AAM02055"/>
        <property type="gene ID" value="MK0842"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0842"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.885"/>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="OMA" id="KTNPIGN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_S3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_S3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_S3_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130251" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="227" mass="25376" checksum="40AB8AE33E4824F0" modified="2002-06-01" version="1">MVMYGENVPVHKKFVQYGMLKTELDEYLEEELGRAGYGGMRLQRVPNATKIIAYVERPAIAIGRRGRNIRRVEEEVQERFPLLGRVSIEVKELPSPELNPRVVARRLASALERGIHFRRAAYGALRRIMNAGAKGAMIILSGKLIGARARTEKFMEGAVKYCGEPGDEYMIEGYVQAVTKPGAIGVTVRIMPPDVELPDELEIRPPEEVEDELKELIGKSEDEAEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX53</accession>
    <name>Q8TX53_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusG</name>
        <name evidence="3" type="synonym">spt5</name>
        <name evidence="6" type="ordered locus">MK0823</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0823"/>
    <dbReference type="EnsemblBacteria" id="AAM02036">
        <property type="protein sequence ID" value="AAM02036"/>
        <property type="gene ID" value="MK0823"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0823"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.865"/>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="OMA" id="FSEIEHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NGN" evidence="4">
        <location>
            <begin position="6"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="163" mass="18419" checksum="D22FF483F7EB9DB7" modified="2002-06-01" version="1">MAETDSTKLYAVRVQAGREEATADMLVMRARRKVKEEGIETGLKAVIAPEELRGYVIIEVEELTDELRDLIHDLPDTRGIVEKPMDFEEIEHYFAPKPEAVEISEGDVVEILSGPFKGEKARVVSVDESRREITVELLEAPVPIPVTVKMDALRLLREEEYEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX56</accession>
    <name>SYFB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">MK0820</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX56"/>
    <dbReference type="SMR" id="Q8TX56"/>
    <dbReference type="STRING" id="190192.MK0820"/>
    <dbReference type="EnsemblBacteria" id="AAM02033">
        <property type="protein sequence ID" value="AAM02033"/>
        <property type="gene ID" value="MK0820"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0820"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.862"/>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="OMA" id="FPGRCAN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000127003" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="276"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="590" mass="67512" checksum="FE1D644780F256BE" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX73</accession>
    <name>Q8TX73_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02016">
        <property type="protein sequence ID" value="AAM02016"/>
        <property type="gene ID" value="MK0802"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0802"/>
    <dbReference type="HOGENOM" id="CLU_651539_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="421" mass="44952" checksum="CC99E217F5C79114" modified="2002-06-01" version="1">MLPMLFTLLLLAGPSQAAELGPADVPGPGTASNPPALALQSDHALIAWKSPEGLGIAVAKADGRVLVKTGIRTPVASYPAAAPWRGERDLATFVLTVPLMTPEGPRIDVDLVEYSDNVCAVLWTGLSVRGDAAVPVRVDDSTFALVYRCEGKLRVRVFGNVDGVWRWITDEQGQPVELKLSAPGPVERFSVSVAGRPVTTYLLVAYETPAGTEVVAVNLTDPAHPTIDGAPIPVPGTRPVLLDNVLAYVGADGVHAALLVKGTAWKIASDVKVADATDVRPAIVSYGVDRYLIVYGGSELRAAEVRIEGTRLGVVREGTLIKEPGYSPTGIGVVRTTERSLEVAGVILAFYARDGEPTVHVEYSEGLGELPTQHRRRPVWIVPLLLAASIGAYIGLKLPVYGQFLWLIISRARREVPKHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD0</accession>
    <name>Q8TXD0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted nuclei-acid-binding protein, consists of a PIN domain and a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0744</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0744"/>
    <dbReference type="EnsemblBacteria" id="AAM01958">
        <property type="protein sequence ID" value="AAM01958"/>
        <property type="gene ID" value="MK0744"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0744"/>
    <dbReference type="HOGENOM" id="CLU_109674_0_0_2"/>
    <dbReference type="OMA" id="RERSSHN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="PTHR12814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="4">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="143" mass="15822" checksum="941D6ACC96B303BC" modified="2002-06-01" version="1">MLGGTQLTTYVLDTSALIKGVPPELLDGPAYTVPEVIEELKDDLSRVRYEVASVRVKEPEDWAVRRARRRAKVTGDLPRLSKTDLKVLALAIELMEEQDVVLVSSDYSVQNVALTLGIRVYGPVHGEVDEVIGPGGRRWRPSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD7</accession>
    <name>DHYS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="1">DHS</shortName>
            <ecNumber evidence="1">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dys</name>
        <name type="ordered locus">MK0737</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXD7"/>
    <dbReference type="SMR" id="Q8TXD7"/>
    <dbReference type="STRING" id="190192.MK0737"/>
    <dbReference type="EnsemblBacteria" id="AAM01951">
        <property type="protein sequence ID" value="AAM01951"/>
        <property type="gene ID" value="MK0737"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0737"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.777"/>
    <dbReference type="HOGENOM" id="CLU_039781_1_0_2"/>
    <dbReference type="OMA" id="EARSWGK"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008612">
        <property type="term" value="P:peptidyl-lysine modification to peptidyl-hypusine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="PTHR11703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134496" description="Probable deoxyhypusine synthase">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <sequence length="297" mass="32475" checksum="489065D5A3131867" modified="2002-06-01" version="1">MKRETFRYRGDMDLDGFLREMERVGALGAGRLGRAARILEKMWSSDDVTVLLTVAGPAVAGGLGELFERLIREGLVDAVITSGANVVHDALDALGGIHVCLGERNVDGYGRVHDTHIPTEAFEKFEHFMREVLSDLSGRVSCRELLWEMGKRLESGFLRAAADEGVPIYSPGILDSMVGLHVWIHSQDHDFHLDLVDDMHHLADLVFEAEELGAIILGGSVPKHFAMGAAMLRGGLDYAVQITMDRPETGSLSGAPLEEGKSWEKVREDAEVATIVGDYLIIFPLLASGVMQRLGIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF6</accession>
    <name>ACDE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit epsilon</fullName>
            <shortName evidence="1">ACDS complex subunit epsilon</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex carbon monoxide dehydrogenase subunit epsilon</fullName>
            <shortName evidence="1">ACDS CODH subunit epsilon</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cdhB</name>
        <name type="ordered locus">MK0718</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase. The precise role of the epsilon subunit is unclear; it may have a stabilizing role within the alpha(2)epsilon(2) component and/or be involved in electron transfer to FAD during a potential FAD-mediated CO oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CdhB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF6"/>
    <dbReference type="SMR" id="Q8TXF6"/>
    <dbReference type="EnsemblBacteria" id="AAM01932">
        <property type="protein sequence ID" value="AAM01932"/>
        <property type="gene ID" value="MK0718"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0718"/>
    <dbReference type="HOGENOM" id="CLU_1656877_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019385">
        <property type="term" value="P:methanogenesis, from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003704">
        <property type="entry name" value="CO_DH_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02552">
        <property type="entry name" value="CO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155091" description="Acetyl-CoA decarbonylase/synthase complex subunit epsilon">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q46G05"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="159" mass="17887" checksum="B26BF2865579C13A" modified="2002-06-01" version="1">MPLPRDPTLIYTDKAEVARAPVLKMVFRRANDSLMIVGPRAVEDEEWRELLMKLREEFRMSAVCTAPYKGNDLGRKMGIVEAATLLQRDAPVLGVHPDLVVFTGSRTDVTDRVLQGLRHARPDLVKVSLNPEYCPSADYSLPTVKRDQFLQELKALVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH5</accession>
    <name>Q8TXH5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01913">
        <property type="protein sequence ID" value="AAM01913"/>
        <property type="gene ID" value="MK0699"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0699"/>
    <dbReference type="HOGENOM" id="CLU_914018_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33383" checksum="8E931BFD0ACCA031" modified="2002-06-01" version="1">MRGQITVDALAAGLISAIILLLVTDVARVVTAHGITSLRTYELYASVDRFAASLTSPSWVGANLTSYDIVWLWSEPLQWTWGGAPLRTGVRMYLAVDAYKWTRYSEGGSLDSPIKVNDGRMMADLVFPNDDLNVSGEAVVKSYDVMLVKRGDTWVYDENLPQVWGNTVSYSGNVPGVKVVWVTVSGDGLYGWVRWLHKRYNQLIQSGLSEEEAYKELAREAISMNSGGNRIVFYSSSGWATDDPVELVRRIVAEGEGSGLSLTIIAIGGRPFTVTLSGSENEIFWNQWTAGLFPVKLRVGVTAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ4</accession>
    <name>EF2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongation factor 2</fullName>
            <shortName evidence="3">EF-2</shortName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mka FusA intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="3" type="primary">fusA</name>
        <name type="ordered locus">MK0679</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The intein interrupts the GTP-binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ4"/>
    <dbReference type="SMR" id="Q8TXJ4"/>
    <dbReference type="STRING" id="190192.MK0679"/>
    <dbReference type="EnsemblBacteria" id="AAM01894">
        <property type="protein sequence ID" value="AAM01894"/>
        <property type="gene ID" value="MK0679"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0679"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.718"/>
    <dbReference type="HOGENOM" id="CLU_265167_0_0_2"/>
    <dbReference type="OMA" id="IEWISHE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0404">Intron homing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000007436" description="Elongation factor 2, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007437" description="Mka FusA intein" evidence="2">
        <location>
            <begin position="35"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007438" description="Elongation factor 2, 2nd part" evidence="2">
        <location>
            <begin position="557"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="273"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="541"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1237"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="616"/>
            <end position="620"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="670"/>
            <end position="673"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="3">
        <location>
            <position position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1257" mass="142071" checksum="2C916583EF9D9B3C" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP5</accession>
    <name>Q8TXP5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0615"/>
    <dbReference type="EnsemblBacteria" id="AAM01830">
        <property type="protein sequence ID" value="AAM01830"/>
        <property type="gene ID" value="MK0615"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0615"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.652"/>
    <dbReference type="HOGENOM" id="CLU_167318_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="11997" checksum="1F9598F6926C6775" modified="2002-06-01" version="1">MKKDELFLLHSVLYYVLEYLKMEGKTREDDVKLYDEFNVKPGHLHKTKLQHKCAVFLLAYVIARAISSELPKAEGLGQRLGKLLEELFEELKRRGEEVPVEPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP8</accession>
    <name>Q8TXP8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted DNA-binding protein containing a PD1-like DNA-binding motif</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01827">
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        <property type="gene ID" value="MK0612"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0612"/>
    <dbReference type="HOGENOM" id="CLU_114051_3_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11378">
        <property type="entry name" value="DUF296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005175">
        <property type="entry name" value="PPC_dom"/>
    </dbReference>
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        <property type="entry name" value="PCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51742">
        <property type="entry name" value="PPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="141" mass="16028" checksum="03EECD1A1A540D90" modified="2002-06-01" version="1">MIEERVEGAELRVVRLEPGERFPESVDTSRWRNSVVVSGKGSVRALRWRLGDTPVFETRGKFELISLEGLLFDDGSYHLHASVSDENGHTLSGHVKGFEVYTTVELVLLRLNARLERGRDPHTGYRELTRVIPSARDTRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR3</accession>
    <name>Q8TXR3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-13 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01812">
        <property type="protein sequence ID" value="AAM01812"/>
        <property type="gene ID" value="MK0597"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0597"/>
    <dbReference type="HOGENOM" id="CLU_380663_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018776">
        <property type="entry name" value="Membrane_prot_PTPS-rel_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10131">
        <property type="entry name" value="PTPS_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="769"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="PTPS_related" evidence="2">
        <location>
            <begin position="68"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="789" mass="87566" checksum="86A4CE0D65A83670" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR5</accession>
    <name>Q8TXR5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted archaea-specific methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0595"/>
    <dbReference type="EnsemblBacteria" id="AAM01810">
        <property type="protein sequence ID" value="AAM01810"/>
        <property type="gene ID" value="MK0595"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0595"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.631"/>
    <dbReference type="HOGENOM" id="CLU_057700_0_0_2"/>
    <dbReference type="OMA" id="LDTCMGL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltranfer_dom" evidence="1">
        <location>
            <begin position="136"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="289" mass="32322" checksum="EC50405B643C43C8" modified="2002-06-01" version="1">MRRYRIRPTDFVAVVSHFEARELLRAVRKGGEIEIQLDLGLHSGKARINGDTVVIEGVELTRDELESMTGWKGAYGLTTDNVYRIEIRAEHYYKLLPVRPGEAPTIEIDGIRMHRTKWTDPWTDAGVKVGKVGVSEGDRVLDVCTGLGYTALRAVERGAEVISVEKDRNVLEIAGANPWSRGLEEVEILLGPAEEVVPSLDDEFDAIVHDPPRLARAGELYSENFYRELLRLLRPGGRLVHYVGAPGSRYRGKDVLAGVSRRLTRVGFEVVDVDRRWGLVTAEKPSARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV3</accession>
    <name>Q8TXV3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RecA-superfamily ATPase implicated in signal transduction</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0556"/>
    <dbReference type="EnsemblBacteria" id="AAM01771">
        <property type="protein sequence ID" value="AAM01771"/>
        <property type="gene ID" value="MK0556"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0556"/>
    <dbReference type="HOGENOM" id="CLU_961763_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="289" mass="32376" checksum="71B58BBFCF3AFEBC" modified="2002-06-01" version="1">MTDRIETRIPGFDEMIGGGLPRRGVTVLAGLPGSARQPLLDNAVWNVLEDGFRVLLLATSTSPYEFLRRSEEYDRRAREAYEKGDLVVLNAFSVRAGIYHRTVGEIIQDLEPHRIRELVPEYDPDVVVIDSIYPMHRGEHEKFLQVISTLKVAAVRNDLAVLAGCALPESALRSPNAEVADVLLETAVHEFRGALVYSLLPIRTLPPHLPKYRAPVSILDDGTVVVHCDKVLDVKAGEIRDVDEYLNDVLNMPESMAREVAEDSEVLGELEEERDDIEKLLEELEEMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY5</accession>
    <name>Q8TXY5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0523"/>
    <dbReference type="EnsemblBacteria" id="AAM01738">
        <property type="protein sequence ID" value="AAM01738"/>
        <property type="gene ID" value="MK0523"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0523"/>
    <dbReference type="HOGENOM" id="CLU_1754711_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007374">
        <property type="entry name" value="ASCH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04266">
        <property type="entry name" value="ASCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ASCH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="148" mass="16896" checksum="7B16AE2EC1338FDD" modified="2002-06-01" version="1">MSVKPKFANLILDGVKNVEVRRWLPGTILRERTCIVYASSPLCAVLGEVTIEEIKKVAIQSEEDLAEIAELAKASEDELRRYLEGRDHAYLITLDGPVRYPNPLPLEDLRRLIKERLNMDFHPNPLFRVDQEVLNVVRSAVGVSDRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY6</accession>
    <name>Q8TXY6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0522"/>
    <dbReference type="EnsemblBacteria" id="AAM01737">
        <property type="protein sequence ID" value="AAM01737"/>
        <property type="gene ID" value="MK0522"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0522"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.556"/>
    <dbReference type="HOGENOM" id="CLU_1998728_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007212">
        <property type="entry name" value="Zf-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04071">
        <property type="entry name" value="zf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="128" mass="15075" checksum="9E45DA840954D04D" modified="2002-06-01" version="1">MARRHFEKLDEKRVREPVGPNKDCEYYPCHFDGQDCTWCFCPLYPCLDEELGEWIKTKNGSEVWSCKDCHLIHRPEPAKVLLRELLSVGNGSVLRGAERLEEDPELKEKVLESVKRADRKHRQHSSRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-08" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ3</accession>
    <name>IF1A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 1A</fullName>
            <shortName evidence="1">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">eIF1A</name>
        <name type="ordered locus">MK0515</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXZ3"/>
    <dbReference type="SMR" id="Q8TXZ3"/>
    <dbReference type="STRING" id="190192.MK0515"/>
    <dbReference type="EnsemblBacteria" id="AAM01730">
        <property type="protein sequence ID" value="AAM01730"/>
        <property type="gene ID" value="MK0515"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0515"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.547"/>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="OMA" id="VPWDFQD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="PTHR21668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145122" description="Translation initiation factor 1A">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <sequence length="111" mass="13083" checksum="93F67811814199A8" modified="2002-06-01" version="1">MGKRIDDETLKKIRLPKEGEIFGVVEKMLGNDRVQVRCVDGKTRVARIPGKMRKRVWIREGDVVLVKPWEFQPERADVTWRYTRVQVDWLKRKGKLDERVTKLLEEIIPGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-11" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY15</accession>
    <name>CARA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="1">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carA</name>
        <name type="ordered locus">MK0491</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY15"/>
    <dbReference type="SMR" id="Q8TY15"/>
    <dbReference type="STRING" id="190192.MK0491"/>
    <dbReference type="MEROPS" id="C26.A33"/>
    <dbReference type="PRIDE" id="Q8TY15"/>
    <dbReference type="EnsemblBacteria" id="AAM01706">
        <property type="protein sequence ID" value="AAM01706"/>
        <property type="gene ID" value="MK0491"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0491"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.521"/>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_2"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112359" description="Carbamoyl-phosphate synthase small chain">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="177"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <sequence length="361" mass="39998" checksum="ACC6F27ADCCC6D6D" modified="2002-06-01" version="1">MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGNYGILPEVGESDRVQVEGFVVRYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKIRTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLVPQVSVREEYRFSDGKPEIVVIDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVRELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALDPDSLRDTPLRVRWVNVNDGTVEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCREH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY37</accession>
    <name>Q8TY37_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01684">
        <property type="protein sequence ID" value="AAM01684"/>
        <property type="gene ID" value="MK0469"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0469"/>
    <dbReference type="HOGENOM" id="CLU_2802252_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="67" mass="7556" checksum="9D6726FF8C080556" modified="2002-06-01" version="1">MRWVPVVAVLVADMTLVWADVARPWLVDRYHGVFSALTILAIALTIFVLGWALSPRGDRDRTPFLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY54</accession>
    <name>Q8TY54_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01667">
        <property type="protein sequence ID" value="AAM01667"/>
        <property type="gene ID" value="MK0452"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0452"/>
    <dbReference type="HOGENOM" id="CLU_2257382_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="38"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="11621" checksum="F2F2A00CBF8C79E6" modified="2002-06-01" version="1">MNLVAEAVRRFKSVDLIRSQILGLKGLLPRKDRAVEVSTGARLVEEAIEVRNAVRSGNREALIEELGDLLIEVEAFLTAHDIDLEEIVERQRTKQRELGQMEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-02-10" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY66</accession>
    <name>SEPS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">O-phosphoserine--tRNA(Cys) ligase</fullName>
            <shortName evidence="1">O-phosphoserine--tRNA ligase</shortName>
            <ecNumber evidence="1">6.1.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical O-phosphoseryl-tRNA(Cys) synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-phosphoseryl-tRNA(Cys) synthetase</fullName>
            <shortName evidence="1">SepRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sepS</name>
        <name type="ordered locus">MK0439</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L-seryl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:25678"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Interacts with SepCysS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.27" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY66"/>
    <dbReference type="SMR" id="Q8TY66"/>
    <dbReference type="STRING" id="190192.MK0439"/>
    <dbReference type="PRIDE" id="Q8TY66"/>
    <dbReference type="EnsemblBacteria" id="AAM01654">
        <property type="protein sequence ID" value="AAM01654"/>
        <property type="gene ID" value="MK0439"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0439"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.468"/>
    <dbReference type="HOGENOM" id="CLU_506822_0_0_2"/>
    <dbReference type="OMA" id="RSHMTSG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043816">
        <property type="term" value="F:phosphoserine-tRNA(Cys) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01674">
        <property type="entry name" value="Sep_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005246">
        <property type="entry name" value="O-Pseryl-tRNA(Cys)_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF38">
        <property type="entry name" value="PTHR11538:SF38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00470">
        <property type="entry name" value="sepS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000363757" description="O-phosphoserine--tRNA(Cys) ligase">
        <location>
            <begin position="1"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="239"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="281"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01674"/>
        </source>
    </evidence>
    <sequence length="544" mass="61614" checksum="4533BF2A4F52343E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY68</accession>
    <name>Q8TY68_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="8">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">trpE</name>
        <name evidence="11" type="ordered locus">MK0437</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0437"/>
    <dbReference type="EnsemblBacteria" id="AAM01652">
        <property type="protein sequence ID" value="AAM01652"/>
        <property type="gene ID" value="MK0437"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0437"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.466"/>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
    <dbReference type="OMA" id="GCVGYLD"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anth_synt_I_N" evidence="10">
        <location>
            <begin position="13"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate_bind" evidence="9">
        <location>
            <begin position="199"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="468" mass="53030" checksum="CC5BB22A64C4E08D" modified="2002-06-01" version="1">MRLTVRELDIDRSPDEVYAALRTLSSHTFLFESAEIGASGRYSFVGFSPALRIECVDGRVRVDVGDPEYADLVVEGRKETDEDHFQLMRRVFSRIPKVEGSGFVGGLVGYISYDVVEDWLDVKSTTVADPEWPSFELCLYDSVVRFDHYEDRVELISVHPDDYEVEWTAEAIEECVREVSGQTAPKVHRTGELKRDLEKEEFEGIVERAKEYIISGDIFQVVLSRRVEVRAVADPLEVYRRLRDINPSPYMYCLEFGERRIIGSSPETLVRLEGDRIITKPIAGTRPRGSTPEEDEELAREMLEDEKELAEHAMLVDLARNDVGKVSRPGTVEVTRLMEIEKYSHVQHIVSEVVGERKEGVTPWDVLRATFPAGTVSGAPKVRAMEIIDELEVYKRGPYAGGVGYVSWTGDMDFAISIRTIFSTGRRWFTQAGAGIVYDSVPENEWFETENKMKAMVGAILEETSSDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY72</accession>
    <name>SURE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">MK0433</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY72"/>
    <dbReference type="SMR" id="Q8TY72"/>
    <dbReference type="STRING" id="190192.MK0433"/>
    <dbReference type="EnsemblBacteria" id="AAM01648">
        <property type="protein sequence ID" value="AAM01648"/>
        <property type="gene ID" value="MK0433"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0433"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.462"/>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_2"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111865" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <sequence length="261" mass="28082" checksum="E3A9568E8AE8D75D" modified="2002-06-01" version="1">MRILITNDDGIASPGLRAAVRACRSVGEVTVVAPATQQSGVGRSISLLEPVRVEEIEVEGVDALAISGTPADAVLIGAFSIMDEPPDLVVSGINLGENVSADVTTSGTVGAALEAYGNGIPAIAISQEVRDARARVDNNAKNVDFTLAIRVLKALLEAIRGANWEGVLNVNVPDPDRWNGEIKVVPLAFTMYRPRIEKRYDPRGRRYYWIDGEIIQDPPEGTDLYELQRGSIVITPLTTDVTGDLDAAENVIKELRRALRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY73</accession>
    <name>Q8TY73_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0432</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01647">
        <property type="protein sequence ID" value="AAM01647"/>
        <property type="gene ID" value="MK0432"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0432"/>
    <dbReference type="HOGENOM" id="CLU_2010114_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="123" mass="14350" checksum="E6E5981C4C93FB40" modified="2002-06-01" version="1">MVVSVLIGSTAAVIIVLIALKTYSKKLRIRELELKVEREKVELVRADLERRKLLDLLFYLPEDTRLMKRVGEIRSLLTRLAEKYMDVETRLTVVELETELKRLEKILKDLERVEGDVRGGENE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY76</accession>
    <name>AROD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type I dehydroquinase</fullName>
            <shortName evidence="1">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroD</name>
        <name type="ordered locus">MK0429</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY76"/>
    <dbReference type="SMR" id="Q8TY76"/>
    <dbReference type="STRING" id="190192.MK0429"/>
    <dbReference type="EnsemblBacteria" id="AAM01644">
        <property type="protein sequence ID" value="AAM01644"/>
        <property type="gene ID" value="MK0429"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0429"/>
    <dbReference type="HOGENOM" id="CLU_064444_2_1_2"/>
    <dbReference type="OMA" id="ATMAMGE"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000138830" description="3-dehydroquinate dehydratase">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <sequence length="215" mass="23919" checksum="A92DAA620A48FB2B" modified="2002-06-01" version="1">MIIATLTGRTIEDMVELAIEAVEQGADALEVRLDYLENLDMSTALRAVRECTRYERVVATLRREEEGGLYKGDEDRRLEILERVSSEADYVDLELDVAEEEIISPSCETIVSYHNFENTPPKEELIGIRDRCAELGDVAKVVTMARGHEDALRILEVVRTAEAPTIGFAMGEEAKYTRVVSVLIGGFATYAAVRKKAAPGQLTVEETRKLLELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY84</accession>
    <name>Q8TY84_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized enzyme of the ATP-grasp superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0421"/>
    <dbReference type="EnsemblBacteria" id="AAM01636">
        <property type="protein sequence ID" value="AAM01636"/>
        <property type="gene ID" value="MK0421"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0421"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.450"/>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="OMA" id="ICLMGET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="CHP00062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00162">
        <property type="entry name" value="TIGR00162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="269" mass="29223" checksum="DCCBCDD7E7F47FB1" modified="2002-06-01" version="1">MDLLKLARGNTVIKFDFEPEVEEPVLVEGLPGIGHVGKLAAEAMIEDLGAEKFAELYSPYFPPHVSVNEDGIVEVMRNEFYVYESEGDEPDIIFLIGEAQAQGELGQHEVTIRILQTVKEFGTEMIFTLGGLGTGTVPTEPKVVGAATHKELIDLLKEHGIEVRSGDKGGNIVGASGLLLGFGKMMGMKGVCLMGVTPGHVIDPRAAMAVVEKLSTILGVEVEADSLKKRAERFEREFVAQLEEMPSALEEAQQEAEEGKPEEDLRYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY89</accession>
    <name>Q8TY89_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01631">
        <property type="protein sequence ID" value="AAM01631"/>
        <property type="gene ID" value="MK0416"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0416"/>
    <dbReference type="HOGENOM" id="CLU_1113874_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="28889" checksum="71333B5F666D64EA" modified="2002-06-01" version="1">MDVRSAERLLEDLKEFAERVNKAFSRINPQIMERRTPRGWLREMVREYFENLGAEVLREACEIAKEDIRSYRELDELLQEIERSTDREEDVLYVRRALLRYMFCRTYTLQRLLVRLYWSLSAGTADVAELVGMLRRMDLFAEEAQLIYPVTPDVEDLREAVKDAIQVIGEEVGAGTEEEDDRLEPEPKLEVCVVRGKLPAFVDPDTGAEVPPSKEGDLLMVGETAARILSERGRRWGGPFAEHVRRWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY99</accession>
    <name>Q8TY99_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin-guanine dinucleotide biosynthesis protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mobA</name>
        <name evidence="2" type="ordered locus">MK0406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0406"/>
    <dbReference type="EnsemblBacteria" id="AAM01621">
        <property type="protein sequence ID" value="AAM01621"/>
        <property type="gene ID" value="MK0406"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0406"/>
    <dbReference type="HOGENOM" id="CLU_1607167_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="1">
        <location>
            <begin position="3"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="165" mass="18579" checksum="40C051E2DAFB85C4" modified="2002-06-01" version="1">MIGAVLMGGKGRRLGGDKPWLTVNGRPIVEWATEMLRRIGCEEVYAVSPRRDGRWDGPWLRDSKGSGPMAGVRAVFREFPNEIVCVTSCDVVFDPRIFREVAEPPCHTRGTLFPFLVRAEDAPEHRTVREFLKNIGSTELEVPATDLDELEDLPRYRALLSRITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA7</accession>
    <name>Q8TYA7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">comD</name>
        <name evidence="2" type="ordered locus">MK0395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0395"/>
    <dbReference type="EnsemblBacteria" id="AAM01610">
        <property type="protein sequence ID" value="AAM01610"/>
        <property type="gene ID" value="MK0395"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0395"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.421"/>
    <dbReference type="HOGENOM" id="CLU_113594_0_0_2"/>
    <dbReference type="OMA" id="IPMGQWT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022502">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03845">
        <property type="entry name" value="sulfopyru_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="18233" checksum="05052ECB6370C3E3" modified="2002-06-01" version="1">MNVDDAIVEALEEAGITFACWLPCSLLDGIIRRLEEHPEIRTVRVSREEEGVGICAGAALAGEKPALIMQNSGLGNSVNALCSLTLTYRLPLLMLMSHRGYLFEDIPAQVGMGKAAPKILENLNLHAFTIERPEELDVIPGAWKLAETAGEPVGVFLSPRLWRQTGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB3</accession>
    <name>RL15E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15e</name>
        <name type="ordered locus">MK0389</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYB3"/>
    <dbReference type="SMR" id="Q8TYB3"/>
    <dbReference type="STRING" id="190192.MK0389"/>
    <dbReference type="PRIDE" id="Q8TYB3"/>
    <dbReference type="EnsemblBacteria" id="AAM01604">
        <property type="protein sequence ID" value="AAM01604"/>
        <property type="gene ID" value="MK0389"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0389"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.415"/>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="OMA" id="KYMQELY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_L15e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_L15e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_L15e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="PTHR11847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127574" description="50S ribosomal protein L15e">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="163"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="197" mass="23356" checksum="AA68324401372C61" modified="2002-06-01" version="1">MDAVSMYKYQREAWKRPKDSYVGELLKERLPKWRKGPSVQRIKRPTRIERARRLGYRAKPGYVVVRVRVPKGGRRKSRPKKGRRPKRMGKNKFSPGKSKQWIAEERAQRKYPNLEVLNSYWVGEDGQYKYYEVIMVDPYHPQIKSDHRINWICQKSQKGRVFRGKTGAGKKARGLRKRGKGAEKVRPSLRAHRRRGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB6</accession>
    <name>RNP2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 2</fullName>
            <shortName evidence="1">RNase P component 2</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp2</name>
        <name type="ordered locus">MK0386</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01601" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01601.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYB6"/>
    <dbReference type="SMR" id="Q8TYB6"/>
    <dbReference type="STRING" id="190192.MK0386"/>
    <dbReference type="EnsemblBacteria" id="AAM01601">
        <property type="protein sequence ID" value="AAM01601"/>
        <property type="gene ID" value="MK0386"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0386"/>
    <dbReference type="HOGENOM" id="CLU_1615315_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="SSF160350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140021" description="Ribonuclease P protein component 2">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="161" mass="18424" checksum="9C319351A71DB9D5" modified="2004-04-13" version="2">MRVRLSSALRPRWRYVTFKVWSERVEALDFGGMKDLVVRALLSVLGPTGTGRIGPWLVRSYRDLNAGILRVRRGQEEEARAALSLYRRDPKLGRVFIEVLGTSGTIKGAERYLSRIPKWDRERVGNREFVLYENGEVDVVEDGRIVAFASFECPLPEENRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC4</accession>
    <name>RPO12_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo12</name>
        <name evidence="1" type="synonym">rpoP</name>
        <name type="ordered locus">MK0378</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYC4"/>
    <dbReference type="SMR" id="Q8TYC4"/>
    <dbReference type="EnsemblBacteria" id="AAM01593">
        <property type="protein sequence ID" value="AAM01593"/>
        <property type="gene ID" value="MK0378"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0378"/>
    <dbReference type="HOGENOM" id="CLU_3094104_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03604">
        <property type="entry name" value="DNA_RNApol_7kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159759" description="DNA-directed RNA polymerase subunit Rpo12">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <sequence length="51" mass="6316" checksum="A2D3C3CF2D7A25A6" modified="2002-06-01" version="1">MYEEEKYEYICMRCGKKVRLDINEDPIRCTHCGFRLVMKPRHPVPRRYKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD2</accession>
    <name>Q8TYD2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01584">
        <property type="protein sequence ID" value="AAM01584"/>
        <property type="gene ID" value="MK0369"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0369"/>
    <dbReference type="HOGENOM" id="CLU_3302716_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="39" mass="4487" checksum="3498E147C2D5B0E5" modified="2002-06-01" version="1">MRLWRLTAVMRLRNVMSRLVLALKEVEGGIIVGLLYDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG3</accession>
    <name>Q8TYG3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0336</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01551">
        <property type="protein sequence ID" value="AAM01551"/>
        <property type="gene ID" value="MK0336"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0336"/>
    <dbReference type="HOGENOM" id="CLU_1431652_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="189" mass="21286" checksum="A32DAEA0E10A7315" modified="2002-06-01" version="1">MFILVTSNFLSFNRYTVTREELFETGYNLVTKLLKDPPRPYHTVKIRVNGKKGKVLTHAGEPVLFVFAGYPGVSEDAKVFVEMNGKEEAIKPGSTVNLDWEDPNEVLSNVTGFFAGSRYHYEQSVVLAGASLTLSTVLPEDLIERLKELGPPKKLPERAGGEELKVRRMTSSEGILSSIRRLLLRFRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYH5</accession>
    <name>Q8TYH5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fragment of predicted dehydrogenase related to phosphoglycerate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01539">
        <property type="protein sequence ID" value="AAM01539"/>
        <property type="gene ID" value="MK0324"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0324"/>
    <dbReference type="HOGENOM" id="CLU_1912354_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="132" mass="13351" checksum="DF619E6CF977E70A" modified="2002-06-01" version="1">MAKILVTDPIHEDALIKALKEGWIAGLACWGVDGEGPAAVLREAYRRVDVHPVRGVVALAVLEDAAGVLLEGLGEGGGAVVGVFPSGVERAIGSASRSVPLRVVVGAVGGVRGVVGGRERRLAGAVLSDPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI2</accession>
    <name>Q8TYI2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted DNA methylase containing a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01532">
        <property type="protein sequence ID" value="AAM01532"/>
        <property type="gene ID" value="MK0317"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0317"/>
    <dbReference type="HOGENOM" id="CLU_1987634_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="125" mass="14347" checksum="5E5F7D685DD97825" modified="2002-06-01" version="1">MKRSPVAELFPEAFLSELTPKEGEVVANPARQEEPVLEFEEGLRKSFEAMRECLPKDGRLTVMFTHRELRAWDTLVRALRDAGFRVNAAWPVHTEAQWSLYQRDKAAARYTLILACDPWEGDGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI3</accession>
    <name>Q8TYI3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01531">
        <property type="protein sequence ID" value="AAM01531"/>
        <property type="gene ID" value="MK0316"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0316"/>
    <dbReference type="HOGENOM" id="CLU_2629741_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="77" mass="8628" checksum="E953E78F7A897D01" modified="2002-06-01" version="1">MINEVYLVLDGCVKAYPSELYLTWSRGAVANLTREPIEQKPQEWSSMLRLTAAKIAPVLKLLCVVLPALLELIKQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK4</accession>
    <name>Q8TYK4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0292"/>
    <dbReference type="EnsemblBacteria" id="AAM01509">
        <property type="protein sequence ID" value="AAM01509"/>
        <property type="gene ID" value="MK0292"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0292"/>
    <dbReference type="HOGENOM" id="CLU_958503_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="3"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="290" mass="32762" checksum="1EC85EB828134130" modified="2002-06-01" version="1">MDLRIAHVSDLHLDESRDKSVLEAAIERVKELHPDVVVVSGDIVKGWRPRHYGETERIFRALDVGKLLAVVPDNHDEMRGGEVVFRKHPYFRRKYREFTVKSPSFGELLMYPIILEGDGLCVIALDSTEADRSDGEVGLDQLLRAEELIREIEPEFIVLTMHHHVTPFPGLIDVSTVLDAGNLREFCVANGVDLVLVGHKHIPRVDYFASKEGGCAVSHAGTLCPEVARYVPPAFNVVDVERGEVVVQVLEYREGEFSEPKLMGRFRIEGEHLRPVELGYDPSKAWMRGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK5</accession>
    <name>DCDB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="1">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">DCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dcd</name>
        <name type="ordered locus">MK0291</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCTP + 2 H2O = diphosphate + dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYK5"/>
    <dbReference type="SMR" id="Q8TYK5"/>
    <dbReference type="STRING" id="190192.MK0291"/>
    <dbReference type="EnsemblBacteria" id="AAM01508">
        <property type="protein sequence ID" value="AAM01508"/>
        <property type="gene ID" value="MK0291"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0291"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.295"/>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_2"/>
    <dbReference type="OMA" id="FENHRYP"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156030" description="dCTP deaminase, dUMP-forming">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="193" mass="21593" checksum="F3CAD69C62DD3168" modified="2002-06-01" version="1">MTVLSDRDIKRALEEGDIVVKPLEEEYLEEALGPASLDLRLGNEFVVFKTLHKPCIDPTVDAGENTERIVIDEDEEFVINPGELVLAVTHEWIEINAPDITGVLHGRSSLGRLGIQAHVEAGYVDPGWRGRLTLELVNFNPMPVKLRPGMRVVQIVFHRLSSPAERTYAESSGKYHGDERPSPSKMHLDFCRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM2</accession>
    <name>Q8TYM2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01491">
        <property type="protein sequence ID" value="AAM01491"/>
        <property type="gene ID" value="MK0274"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0274"/>
    <dbReference type="HOGENOM" id="CLU_1021620_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="272" mass="30861" checksum="E858657782FA8E8E" modified="2002-06-01" version="1">MKPPPEEAWIGRPVRPAQGFHTRVSMIAGVMLGGPADVEFPRMAHPLELPHYLEIDGDTWRRLRCRLESLEDRSPDLGDAVREYIREAFRCITKPDFATALKACTPVIALDTPLWRDARSVVRAIARLLTRVTGVEVQPPPDIAVELRATLYPIAERALVSTLLAQAVAHRIRSLGLRSEYLSAYLDAPSGESPPRELGDVSDLYATLVKDFDGYLARVLRLNRYVTVTAGRRFVLTPDDYDPTWRGYLQGLRRFWENRRGMEEIIRQWGLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM5</accession>
    <name>SYFA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">MK0271</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYM5"/>
    <dbReference type="SMR" id="Q8TYM5"/>
    <dbReference type="STRING" id="190192.MK0271"/>
    <dbReference type="EnsemblBacteria" id="AAM01488">
        <property type="protein sequence ID" value="AAM01488"/>
        <property type="gene ID" value="MK0271"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0271"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.274"/>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000126811" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <sequence length="524" mass="60078" checksum="86BADBAA91C2839D" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ2</accession>
    <name>Q8TYQ2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0240"/>
    <dbReference type="EnsemblBacteria" id="AAM01457">
        <property type="protein sequence ID" value="AAM01457"/>
        <property type="gene ID" value="MK0240"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0240"/>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="PTHR42961"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="290" mass="31825" checksum="CB9C315DED342DEB" modified="2002-06-01" version="1">MSPLAEGNEPREGHPHHPSREELEKRLEKEREAIEKNLESVEHVLVVMSGKGGVGKTTVSVNLALALAEDDEVGILDLDIHGPNVPEQLGVTEPPQGTPAGLFPLSGYRDVKVMSIGTMLEREDLPVLWRGPRKSGFIREILVKTRWGDLDYLIIDMPPGTGDEVMTALQMLPEDARNVLLVASPESLAFSDVVKAGEAVDKLEARLIGIVSNMHGIVCPECGSTIEYFSDDYSEKLAERFDTEVLARIPLDPEAKRKAEEEGKPFVIAAPDSRVSEAFSELAEAVRDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR1</accession>
    <name>Q8TYR1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0231"/>
    <dbReference type="EnsemblBacteria" id="AAM01448">
        <property type="protein sequence ID" value="AAM01448"/>
        <property type="gene ID" value="MK0231"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0231"/>
    <dbReference type="HOGENOM" id="CLU_109226_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022285">
        <property type="entry name" value="CHP03879_regulat_dom_put"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03879">
        <property type="entry name" value="near_KaiC_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="119"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="180" mass="20473" checksum="DC888840827BE7D9" modified="2002-06-01" version="1">MAESVKEVIDLTEIPFQPMDRQEIHQLETVLLVATLFRPKVLEMIHEQKFLTWVDSLAVAASALARQKAGYTISEIAEELGRTEATIRKHLQGETKAGELVLETYEMLKSGELQIVTGVEDIKEKLRVEEEKVERLKSEMEEVSTTISRVVEGLRECCTKMEDALEELEGLAEKLEELKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS0</accession>
    <name>Q8TYS0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TOPRIM-domain-containing protein, potential nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAM01439.1"/>
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    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01439">
        <property type="protein sequence ID" value="AAM01439"/>
        <property type="gene ID" value="MK0222"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_965121_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="124"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="291" mass="33153" checksum="0F3581C31FD78F46" modified="2002-06-01" version="1">MARDGRRKSPVDVRIIVEGAADAETISKVIQRMALGGEYNITVTSIIPTTHAHIARRTAEGADLVLIATDADKPGRKLAKKFQEELRGVVGRVERVKMPIGHDVEHVDLEIVEKELRSALVRAGLKSLRDIKELREEIKELQEEIEEKEELIEELEEKESELEELRERLKEIEKEKALLEEERDRLLDEVERLRDRLEELEEELESADHLRIMDLESVCEEAELSPEDVEPEVLEELGEELEIPIVVGSTRIAAPSREDAVRVLKIYKLARKVVDGEGEEENETTPGDPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS8</accession>
    <name>Q8TYS8_METKA</name>
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        <submittedName>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        <property type="project" value="UniProtKB-KW"/>
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            <begin position="7"/>
            <end position="131"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AAM01431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="139" mass="15365" checksum="C3B44D1725F78867" modified="2002-06-01" version="1">MSGVKRVEFDARLLDSLLEASDKNHPDEFFAMLGGSIDAETITIDSLIVVPFEASDSGAIFDLLSVHTCDVIGTFHSHPYGDPVPSEDDLMLFKRLGAVHAIAAYPYTPDRVEFYDKSGRNITPVVEVRYTADEEANNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU7</accession>
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            <fullName evidence="2">HD superfamily phosphohydrolase</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">MK0194</name>
    </gene>
    <organism evidence="2 3">
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="185"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="419" mass="47133" checksum="23A2A63906F30439" modified="2002-06-01" version="1">MGRIIRIPVHGFVELQGAEERVLDSPEVQRLRRVRQLGLAELVYPGATHTRLEHSLGVKYLCDRVMEIHWEELKRNVPDVGKFSRSYIEEVLGLAGLLHDVGHPPFSHVPEPLLEEELGVDHEDIGRAVAKVVLERADAMDVEVTLEVAFGPGDGWTGVLHSVIAGNLGVDRLDYLMRDSLHASVEYGRIELDRLLHTLELGDPVVVHEKGLEAAESVLISRYHMYRAVYFHKTCRAGDAMLLWAMHVAAEDGDLDLRIFDPDRLSRSEDALSAFQSMDDSALLRALEENPESREFVEVLKNRRLYKCVAEYQAAVEPRHGELFKVAVETSEEHLGESFGVLLDSSKVVPYDPSSDEVIVRTRNGDVPLSEVSDIVATLKERTLSEIPTVRVYVRPETRDDESRVRRIVEHVKSALPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV7</accession>
    <name>Q8TYV7_METKA</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="1" type="ordered locus">MK0184</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
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            <dbReference type="PubMed" id="11930014"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="55" mass="6093" checksum="2BD709849EB6DABC" modified="2002-06-01" version="1">MIWPDLTLGTARITGTTSGTHNVRAVRDRLWAGLMLCTNRRPVIALAYVEALKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW3</accession>
    <name>Q8TYW3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coenzyme F420-reducing hydrogenase, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
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        <lineage>
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            <taxon>Methanopyrales</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0178"/>
    <dbReference type="EnsemblBacteria" id="AAM01395">
        <property type="protein sequence ID" value="AAM01395"/>
        <property type="gene ID" value="MK0178"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0178"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.178"/>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="OMA" id="QNNLAMN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="434" mass="48453" checksum="68E1089DB995ED8A" modified="2002-06-01" version="1">MAEIKIHPLTRVEGHGEVIIEVEDGEVTDVKFAVLAVRGFEKFVQGRPAEDVPYIVSRICGICQTAHHLAACKAVDACFDAEPPEGGHKIRWLMHIGNMIHSHALHFYFLAAPDYVVGPDADPLQRNVVKIVKDDPEVGKIAIELRRYGQDIVEATGGKAIHPVTGIPGGVSSPVDPESRDDLLNRAEEMIEMAYEGAKVGIEAIKETLEEYREKHDIDLLETLGNIETYHMGLVSDGDKHEFYDGEVKVVDPNGEEVTRFEPQEYQDVIAERTIEYSYVKHPYLKDVGYPDGIYRVGPGARLNVCSEMKTERAQELYEEYVDEFGECVNYSLTINWARLVELVAACEEAKMLLEDDVITEEDECKEDYEPKAGEGVGIVEAPRGTLIHHYVTDDEGRVKEANLIVATTHNVPAIELALKETAKKLEDEIVELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX3</accession>
    <name>Q8TYX3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">MK0168</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsA/MreB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0168"/>
    <dbReference type="EnsemblBacteria" id="AAM01385">
        <property type="protein sequence ID" value="AAM01385"/>
        <property type="gene ID" value="MK0168"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0168"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.167"/>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_2"/>
    <dbReference type="OMA" id="MVICIPS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0133">Cell shape</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="393" mass="42760" checksum="EE729AAB18B6470E" modified="2002-06-01" version="1">MPLWKRKKKEETKQEKKIEEEKKENNEKKASKLPTSIQEALSEEPEIEIDEGLEALGIDLGTMNTVVARPAEEEFLVKQFPSVVAVKKGTNRVLAIGEEARRMLGRTPEDIVAVRPMRHGVIESLEYAKFIVQYAIELGSDNSPEEIERVAVGVPGDASEVEREAIEEATSDVGMDKDNVIVINEALAAAIGAGLPIAEPDGTMVIDIGAGSTDIAVISLGGITDQETMRVGGDNIDQNIVDLVEEEFGVRIGIHEAERAKVEVGKVFTETEDIEDKEIEVVGKDIETNKPKEITIDSELVAKAAEPVVQEIIRAIESILDRLPPELVPGVYENTVLVGGTSLMRGLRARIEEETDVPAELVDDPLTVVAKGAAIVAAEPKTLEPEIRLKALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY8</accession>
    <name>Q8TYY8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-hexulose-6-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sgbH</name>
        <name evidence="3" type="ordered locus">MK0153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0153"/>
    <dbReference type="EnsemblBacteria" id="AAM01370">
        <property type="protein sequence ID" value="AAM01370"/>
        <property type="gene ID" value="MK0153"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0153"/>
    <dbReference type="HOGENOM" id="CLU_851561_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OMPdecase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="326" mass="35141" checksum="CBEBF70E58E8D174" modified="2002-06-01" version="1">MLTQVALDLTSLPKAIEIAEASVEAGIHVLEVGTPLIKAEGSRAIERLAEEFPERPIVADTKTMDVGALEAELSIKHGANLGCVLGAAPEETIRSFVTRAHELCAGALVDTIGVNPVDVLSKLKGLEEFPDYLLLHAAIDEPITGEELLHKFGVDRCPARTAVAGGLTPRKIEELDGVDLVIVGGYITSSEDPVKAAEAVVEAAGVEPYDFRPDEDQREALLGIRKHSTVGIVVKDSERVSEVLNAAIRLVEWVRNEPSLRTVGDDDSDLVPKFEGDGRDLTLILGAEEFDREDLESIAKESVKTILVAEEHVLKLYGVAEAVYEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ12</accession>
    <name>GATE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="1">Glu-ADT subunit E</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatE</name>
        <name type="ordered locus">MK0129</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ12"/>
    <dbReference type="SMR" id="Q8TZ12"/>
    <dbReference type="STRING" id="190192.MK0129"/>
    <dbReference type="EnsemblBacteria" id="AAM01346">
        <property type="protein sequence ID" value="AAM01346"/>
        <property type="gene ID" value="MK0129"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0129"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.128"/>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="OMA" id="SGFQRTM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="PTHR11659:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140073" description="Glutamyl-tRNA(Gln) amidotransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <sequence length="640" mass="71622" checksum="F819F1194B8AFA72" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ41</accession>
    <name>Q8TZ41_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="6">1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ribD</name>
        <name evidence="11" type="ordered locus">MK0098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0098"/>
    <dbReference type="EnsemblBacteria" id="AAM01315">
        <property type="protein sequence ID" value="AAM01315"/>
        <property type="gene ID" value="MK0098"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0098"/>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="OMA" id="CECGQEV"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="10">
        <location>
            <begin position="4"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="25540" checksum="F36D8F176D064BBE" modified="2002-06-01" version="1">MPRPKVLYNVGMTADGKVVTAAGDSRISGEEDLKEVHRLRAEHDAVAVGINTVRKDDPMLNVRLVEGEDPIRVVFDTECSIPLDCRLVRTARDIPTVVLCAEADPGRVEKLEKRGVKVEEVGACEDGVDVERGLELLYDMGVRTLLLEGGPTLAWSFLKRGLIDEFRVAVAPVLVGGSDALTPVEGEGFPRVDLGVGLELKRVERVGRDVVLWYEVSGSAADLASEHEEARGRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ59</accession>
    <name>Q8TZ59_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate:ferredoxin oxidoreductase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">porG_1</name>
        <name evidence="3" type="ordered locus">MK0080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0080"/>
    <dbReference type="EnsemblBacteria" id="AAM01297">
        <property type="protein sequence ID" value="AAM01297"/>
        <property type="gene ID" value="MK0080"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0080"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.81"/>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="OMA" id="PDYVIVQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="10"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="183" mass="19988" checksum="A4577C250A2DA6DF" modified="2002-06-01" version="1">MIEIRIHGRGGQGAVTAAEILAIAAKEDGKYSQAFPFFGVERRGAPVTAFARIDDEFIKIRSQIYEPDHVIVLDSSLLAVVDVTEGLPEDGLIVINAREEELDKIMEKFEDSDVYTVDATQIALDELGVPIVNTAMVGAYLKASDILTLDAVKKAIHARFSGEIAEKNVRAVERAYREVKAVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ66</accession>
    <name>NADA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Quinolinate synthase</fullName>
            <ecNumber evidence="2">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">nadA</name>
        <name type="ordered locus">MK0073</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:25889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ66"/>
    <dbReference type="SMR" id="Q8TZ66"/>
    <dbReference type="STRING" id="190192.MK0073"/>
    <dbReference type="EnsemblBacteria" id="AAM01290">
        <property type="protein sequence ID" value="AAM01290"/>
        <property type="gene ID" value="MK0073"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0073"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.74"/>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155804" description="Quinolinate synthase">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O57767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="292" mass="32529" checksum="F457A07A92E84D8F" modified="2002-06-01" version="1">METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDREVVVPDHRAACGMAMRLRAKELREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVVSSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPVHHSLSPSDLRELCSQHPDAAVVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPGHMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ75</accession>
    <name>Q8TZ75_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01281">
        <property type="protein sequence ID" value="AAM01281"/>
        <property type="gene ID" value="MK0064"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0064"/>
    <dbReference type="HOGENOM" id="CLU_1029011_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="31013" checksum="CC3E6E6F2BBD7ED5" modified="2002-06-01" version="1">MPAGPVHLVYGVLLAVTSYPQNPEYGGMIVYASLAPDFATAAYNDRYSDLRYVDYRWAHDPTHVYDYASVIVERTTSRTLQGVDEKVALWCAETGIASHLLLDLYTHVKTSPIKEWREQAGIRTVLQEFYYPWQENAVEFARWALGRAIRVFGNPDEFWRYVDGACAVAEQLMGDYVISAYLRRLSERYFGDPDLHLADWLRWFYRNYGWEENATDAFIIDGVRIPADPVELLSWISQWDPRVVPMRPPQSGGGGSLLLPVFPVPPVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVE7</accession>
    <name>F1SVE7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histone H3/H4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hht1_3</name>
        <name evidence="6" type="ordered locus">MK1677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="F1SVE7"/>
    <dbReference type="STRING" id="190192.MK1677"/>
    <dbReference type="EnsemblBacteria" id="AAM02890">
        <property type="protein sequence ID" value="AAM02890"/>
        <property type="gene ID" value="MK1677"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1677"/>
    <dbReference type="HOGENOM" id="CLU_1700291_0_0_2"/>
    <dbReference type="EvolutionaryTrace" id="F1SVE7"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="5">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBFD_NFYB_HMF" evidence="5">
        <location>
            <begin position="85"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="154" mass="17003" checksum="9AF9E302CBB41C60" modified="2011-05-03" version="1">MAVELPKAAIERIFRQGIGERRLSQDAKDTIYDFVPTMAEYVANAAKSVLDASGKKTLMEEHLKALADVLMVEGVEDYDGELFGRATVRRILKRAGIERASSDAVDLYNKLICRATEELGEKAAEYADEDGRKTVQGEDVEKAITYSMPKGGEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>P58839</accession>
    <name>G3P_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="1">GAPDH</shortName>
            <ecNumber evidence="1">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gap</name>
        <name type="synonym">gapA</name>
        <name type="ordered locus">MK0618</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58839"/>
    <dbReference type="SMR" id="P58839"/>
    <dbReference type="STRING" id="190192.MK0618"/>
    <dbReference type="EnsemblBacteria" id="AAM01833">
        <property type="protein sequence ID" value="AAM01833"/>
        <property type="gene ID" value="MK0618"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0618"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.655"/>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="OMA" id="NAIVPNP"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145724" description="Glyceraldehyde-3-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="350" mass="38367" checksum="F9BCB72702E22E02" modified="2002-05-27" version="1">MADVSVLINGYGTIGKRVADAVDAQRDMEVLGVVKTSPDYLARLAVEEYGYPLFVPEDRVERFEDAGIETEGTVEDVVLNAEDYGLDVVVDCTPEGIGARNKETLYEKAGVKAIFQGGEEAEVAEVSFVAQCNYEEALGADYVRCVSCNTTALCRTLGTLKEEFELGRVYVTIVRRAADPHQVKKGPINAIAPNPVTVPSHHGPDVKTVMPDIDITTAAVKVPTTLMHMHVVRVELKEEVTSDDVIDAFEEARRIWVVPHGEGLGSTAELIELGRDLGRKRYDLYEILVWEESINVEDGVLYYMQAVHQEADVVPENVDAIRAMTELEEDPEASMDATDSALGVLNSPPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>P58855</accession>
    <name>SIHH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-inosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="1">SIHH</shortName>
            <ecNumber evidence="1">3.13.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0368</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + S-inosyl-L-homocysteine = inosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:59828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:59829"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.9" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58855"/>
    <dbReference type="SMR" id="P58855"/>
    <dbReference type="STRING" id="190192.MK0368"/>
    <dbReference type="PRIDE" id="P58855"/>
    <dbReference type="EnsemblBacteria" id="AAM01583">
        <property type="protein sequence ID" value="AAM01583"/>
        <property type="gene ID" value="MK0368"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0368"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.391"/>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000117005" description="S-inosyl-L-homocysteine hydrolase">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="156"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="219"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="298"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <sequence length="424" mass="47016" checksum="3DE7B10A3B44E1D5" modified="2002-06-06" version="1">MVVVKEGEYAIRDPSLAPKGRDMIEWARDHMPVLGAIRERFEEERPLEGITVGMTLHLEAKTAVLVETLMAGGAEVAITGCNPLSTKDEVAAALVEEGVHVYAWRGETEEEYYQNIDRVLSHEPDIIVDDGADCIARVHTEFPDLAERVIGATEETTTGVNRLHAMHREGVLKFPVIAVNDAKTKYLMDNRYGTGQSALDGLMRATNILLAGKTVVVVGYGWCGRGIARRARGLGANVIVVEVDPIKAMEAIFDGFRVMPMDRAAEEGDIFITATGNRDVIRGEHIEKMKDGVILANAGHFDVEIDKEYLEEHCEEKIDRRGGLVTEYRMPDGKRVYLIAEGRLVNLAAGEGHPIEIMDISFALQALSVEVLAKEGKEMEPGVYKVPKDVDKRVAELKLESMGIELEELTPEQREYMKSWEEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUR6</accession>
    <name>Q8TUR6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type phosphate transport system, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pstA</name>
        <name evidence="10" type="ordered locus">MK1688</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1688"/>
    <dbReference type="EnsemblBacteria" id="AAM02901">
        <property type="protein sequence ID" value="AAM02901"/>
        <property type="gene ID" value="MK1688"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1688"/>
    <dbReference type="HOGENOM" id="CLU_023674_0_0_2"/>
    <dbReference type="OMA" id="INIALEM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="74"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="369"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM02901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="594" mass="63625" checksum="36A6FFB70B6740CB" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUS7</accession>
    <name>Y1676_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein MK1676</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK1676</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUS7"/>
    <dbReference type="SMR" id="Q8TUS7"/>
    <dbReference type="STRING" id="190192.MK1676"/>
    <dbReference type="PRIDE" id="Q8TUS7"/>
    <dbReference type="EnsemblBacteria" id="AAM02889">
        <property type="protein sequence ID" value="AAM02889"/>
        <property type="gene ID" value="MK1676"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1676"/>
    <dbReference type="HOGENOM" id="CLU_146474_0_0_2"/>
    <dbReference type="OMA" id="LHNLKSN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000144225" description="UPF0216 protein MK1676">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <sequence length="147" mass="17718" checksum="1B8E8BC609590A37" modified="2002-06-01" version="1">MRDESRLDKYVIKEILRINRHLPRRRKTLEELLREERPHVVNRDGTKHYFDRDELERLADILPRYLHGRLKLPILIELGYSGAAVIRGKAEVRVVCEVLGEEWRFSQDRVELNMLDVRKLRREFPTATQYMFSTEYIMGRPKVERRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU0</accession>
    <name>Q8TUU0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted sugar phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1663</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1663"/>
    <dbReference type="EnsemblBacteria" id="AAM02876">
        <property type="protein sequence ID" value="AAM02876"/>
        <property type="gene ID" value="MK1663"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1663"/>
    <dbReference type="HOGENOM" id="CLU_094236_1_0_2"/>
    <dbReference type="OMA" id="HIFLQGA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="PTHR43443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="180" mass="19989" checksum="C0A16336D16D370B" modified="2002-06-01" version="1">MDRIRKIMRREAEVIEYACNNLPEEDVKKALDLVVEKIRNDRGIFIVGMGRTGLIGECFAVRLVQMGARCYVVGHSTERAIKPDDLLIALSVSGNTAFVNYAADVAKDEGADVLAVTMNADSKIAEKADVVVVLPEPEEIILRTFSEMLMLSFLDGFTAQLAKELGVDESDMWERHAKIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU3</accession>
    <name>RL21_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L21e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl21e</name>
        <name type="ordered locus">MK1660</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUU3"/>
    <dbReference type="SMR" id="Q8TUU3"/>
    <dbReference type="STRING" id="190192.MK1660"/>
    <dbReference type="EnsemblBacteria" id="AAM02873">
        <property type="protein sequence ID" value="AAM02873"/>
        <property type="gene ID" value="MK1660"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1660"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1824"/>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="OMA" id="KGMPHRR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_L21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_L21e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_L21e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_L21e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="PTHR20981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000149690" description="50S ribosomal protein L21e">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="102" mass="11873" checksum="E8D23E55DA841F68" modified="2002-06-01" version="1">MVRRSKGFRSRTRKKLRKKPRERGLSPLGPMTQEFEEGQKVHIVIDPSVHKGMPHPRYHGRTGEVVGRQGRAYIVKIRDGGKEKKLIVYPEHLKPQEQPELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW4</accession>
    <name>Q8TUW4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1639"/>
    <dbReference type="EnsemblBacteria" id="AAM02852">
        <property type="protein sequence ID" value="AAM02852"/>
        <property type="gene ID" value="MK1639"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1639"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1802"/>
    <dbReference type="HOGENOM" id="CLU_018825_1_0_2"/>
    <dbReference type="OMA" id="YINAMVG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="AcnX_catalytic_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcnX" evidence="1">
        <location>
            <begin position="6"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="359" mass="38985" checksum="F8C05E57EDC13E3D" modified="2002-06-01" version="1">MGCPGLYLTKEEERILDGEEGELKAKLMEVLVKLGDVFDAERLVRPASVHVSGVSYGTIGDAGLRFLRKVAEAGLRVSVPTSVNPCGICLDGNLPVDEEFERKQREIMDALESLGVASVYTCVPYQQGFQPSRGDLLAWGESSAVFVANTYYGARANREGAPATVAAAVVGRIPEYGMHLDENRVAEYEVVVEFEPRNDFEWSALGYYLGEALDGIPVLKLPTVPSLSNVKYMGAAAAASGALAMAYIPGITPEEPRMDCPERIEVEREDVFDLVEERFGTEREGFAFTGCPHRPDGEIPRFDGCAICCPANATLDGYRLRGTCPVVAPIEDVHDVVFTDSLKAAHYLASRVEVNVGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY0</accession>
    <name>Q8TUY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted RNA-binding protein containing KH domain, possibly ribosomal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK1621</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1621"/>
    <dbReference type="EnsemblBacteria" id="AAM02834">
        <property type="protein sequence ID" value="AAM02834"/>
        <property type="gene ID" value="MK1621"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1621"/>
    <dbReference type="HOGENOM" id="CLU_095994_2_1_2"/>
    <dbReference type="OMA" id="FKPREGW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="5"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="105" mass="12359" checksum="294EB78F8AE9D5AD" modified="2002-06-01" version="1">MTQVKRLSGKERRALRARAVLLDPVVRIGKKGLTSGVIQEVDRQLEERGLIKVRFERNILRRYDRKELAEELARKVNAELIDVRGRTAVLFRPREGWRRFHGLSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY7</accession>
    <name>PFDA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pfdA</name>
        <name type="ordered locus">MK1614</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY7"/>
    <dbReference type="SMR" id="Q8TUY7"/>
    <dbReference type="STRING" id="190192.MK1614"/>
    <dbReference type="PRIDE" id="Q8TUY7"/>
    <dbReference type="EnsemblBacteria" id="AAM02827">
        <property type="protein sequence ID" value="AAM02827"/>
        <property type="gene ID" value="MK1614"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1614"/>
    <dbReference type="HOGENOM" id="CLU_091867_1_3_2"/>
    <dbReference type="OMA" id="ELQNQFM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PTHR12674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00293">
        <property type="entry name" value="TIGR00293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153676" description="Prefoldin subunit alpha">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="157" mass="17446" checksum="B20BDA8CC978DFA1" modified="2002-06-01" version="1">MAEKKNEQEIQQELQRLIAEINRLQGQMEAINAQIDLIESSISELNRVEETLKGVKELEGDEEVLVPVGAQSFVRACVTDTERVIVGIGAGVAVERTIDEALESIDDQRQELEKARAEAQQKLQELAQELQEKQRKAQELAQQLEGAQRIAQQSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ0</accession>
    <name>Q8TUZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02824">
        <property type="protein sequence ID" value="AAM02824"/>
        <property type="gene ID" value="MK1611"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1611"/>
    <dbReference type="HOGENOM" id="CLU_2476025_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="87" mass="9975" checksum="5CC4C6462D217CF8" modified="2002-06-01" version="1">MKKTIESLGDIGDVLAEEKKNTVELRIVECPFSDVKRELLKRDKVPVVCPFASLVLKATEETFGVRMRTASIDIDEEECRFVMERLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ5</accession>
    <name>ARGD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylornithine aminotransferase</fullName>
            <shortName evidence="1">ACOAT</shortName>
            <ecNumber evidence="1">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argD</name>
        <name type="ordered locus">MK1606</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="1">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUZ5"/>
    <dbReference type="SMR" id="Q8TUZ5"/>
    <dbReference type="STRING" id="190192.MK1606"/>
    <dbReference type="EnsemblBacteria" id="AAM02819">
        <property type="protein sequence ID" value="AAM02819"/>
        <property type="gene ID" value="MK1606"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1606"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1769"/>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_2"/>
    <dbReference type="OMA" id="MVPNYNP"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112822" description="Acetylornithine aminotransferase">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="216"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <sequence length="389" mass="42025" checksum="AD72B13CCFE962F9" modified="2002-06-01" version="1">MDARELIDKYHMNTYSRFPVTLVPGEGARVWDDEGNEYIDLVAGIAVNVLGHCHPAVVEAVKEQVERLIHCSNLYYNEPQAEAARLLAEAAPKDLNKVFFCNSGTESVECAIKLARKFTGCTKFIAFEGGFHGRTMGALSATWKPEFREPFEPLVPEFEHVPYGDVNAVEKAIDDDTAAVIVEPVQGEAGVRIPPEGFLRELRELCDEHGLLLIVDEVQSGMGRTGQFFAFEHEDVLPDIVCLAKGLGGGVPVGATIAREEVAEAFEPGDHGSTFGGNPLACAAVCAAVSTVLEENLPEAAERKGKLAMRILSEAEDVVEEVRGRGLMMGVEVGDDERAKDVAREMLDRGALVNVTSGDVIRLVPPLVIGEDELEKALAELADALRASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV48</accession>
    <name>Q8TV48_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-38 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02765">
        <property type="protein sequence ID" value="AAM02765"/>
        <property type="gene ID" value="MK1552"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1552"/>
    <dbReference type="HOGENOM" id="CLU_648319_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="423" mass="46483" checksum="5D3041DB93C7CE7B" modified="2002-06-01" version="1">MLRPFSILAFIMVLGGAAHAISVQEYTWELTAINIKTAEGVIKAKFSKPQTVHFLVPEPDYDQVDFGRDVDGDGIPDHMWCGELASLTLYNVAVAKGWWKPIAGNELTPEDLEKREEWFAKTTNIPVEDPQYDAPNASTDPPWFPALISLAADPEHPNAIGVVLYAPLVTVSRWFVVVPPMMHAVVVMGATSNGRYFVVKDCSATQGPDYDWKHDYYLVPAYSLELNISRAIQDVFNPLEQYSSTMPVLFFPIPTDGKLPPGLDEFFQGERTVDVGGGLKVRFYVFLASTLSDVLEICRHLAEAGVPVMCVVKEPFAWLAYQGQVLEVPLVGTHVLRILSVPITAVEIAQPTNSNGAETQNQPTQPKVSNAEGEVEPKENESEANSQASPTSSKRRKIPLVPIVPALPRGTNRGRDSDGQVEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV53</accession>
    <name>HYPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hypA</name>
        <name type="synonym">hybF</name>
        <name type="ordered locus">MK1547</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV53"/>
    <dbReference type="SMR" id="Q8TV53"/>
    <dbReference type="STRING" id="190192.MK1547"/>
    <dbReference type="EnsemblBacteria" id="AAM02760">
        <property type="protein sequence ID" value="AAM02760"/>
        <property type="gene ID" value="MK1547"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1547"/>
    <dbReference type="HOGENOM" id="CLU_126929_2_0_2"/>
    <dbReference type="OMA" id="ILLCPCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="PTHR34535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00100">
        <property type="entry name" value="hypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000129078" description="Hydrogenase maturation factor HypA">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <sequence length="142" mass="15806" checksum="7C68A0CD4970B7A6" modified="2002-06-01" version="1">MHELSVAQSVLETVLDVARKRGAERVLSVRLRIGEFTLLNPEQLRFCLEVLAEGTPVEGAKFEIEIERGYFKCAECGHRWRPEDESLKDPSLHTAFDLSELTELLDLKCPKCGSRAVKLDGGDACSIESVRLEVPGEQHAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV59</accession>
    <name>Q8TV59_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02754">
        <property type="protein sequence ID" value="AAM02754"/>
        <property type="gene ID" value="MK1541"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1541"/>
    <dbReference type="HOGENOM" id="CLU_701346_0_0_2"/>
    <dbReference type="OMA" id="PYTYGVK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="36201" checksum="5F6BB99F8CEF8C54" modified="2002-06-01" version="1">MSAYSLPWYSVWAHPFIAGKAIEEYGAVAGLPAVFLGIVPSPAERWVRGDYKVEYGWIVANYQGPGVVLIRGSHLYVKKPGLVVWGRKVPKVVAVKVGSDEIEVEGKRMTFEEAAKKYGWEKLKYLKKADVGERATLVYELKFTDGRRPMDENEVKKVFGEAAYRRMVEMADYSAVVVWIGKYRKKLIGHAETTMEGIGHLDNLRVVNAARMCRGWNGVIVPPHTWTHGKRQYFDVFEVPGYEKAAHGCCPPARALRDACLDAGLPKPKGIDMGVHPMEYGFHPTEGVVVYNTKPYPILIEIGFKGKPKIGGIIFCNIYALLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV73</accession>
    <name>Q8TV73_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Site-specific DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dcm</name>
        <name evidence="4" type="ordered locus">MK1520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1520"/>
    <dbReference type="REBASE" id="5982">
        <property type="entry name" value="M.MkaORF1520P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02733">
        <property type="protein sequence ID" value="AAM02733"/>
        <property type="gene ID" value="MK1520"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1520"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1680"/>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="OMA" id="EPFTGAN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="315" mass="35692" checksum="EB16551E446AD0E1" modified="2002-06-01" version="1">MKPKVVDLFCGAGGFSRGFKEAGFKILGGVENNPAPAATYRENFPEAEVIERDIQRVDSEEIVDELGEPDVIIGGPPCEPFTAANAERKPNPLDRLYDDPVGRLVLHFVRIIGDLQPEVFVMENVPAIMEGPLEKALRKEFARAGYDEIHFNVLQAVHYGVPSYRRRVFISNVRIDPEPTVERPKTVWDAIGDLPSPDSEVPNHELRPLSKRKLRRIRRLRWGEALSVIRGARGSFKNWLRLHPFKPAPTVMGGSRFIHPFEDRLLTVREQARLMSYPDDHVFEGGYETQYDQVGESVPPELARVIAEEVRDHLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV85</accession>
    <name>METK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine synthase</fullName>
            <shortName evidence="1">AdoMet synthase</shortName>
            <ecNumber evidence="1">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mat</name>
        <name type="ordered locus">MK1508</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV85"/>
    <dbReference type="SMR" id="Q8TV85"/>
    <dbReference type="STRING" id="190192.MK1508"/>
    <dbReference type="EnsemblBacteria" id="AAM02721">
        <property type="protein sequence ID" value="AAM02721"/>
        <property type="gene ID" value="MK1508"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1508"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1666"/>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="OMA" id="IGHPDSI"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="PTHR36697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150029" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <sequence length="397" mass="44075" checksum="83AFFDEAC30FD07E" modified="2002-06-01" version="1">MVRNIVVETMKRSPFTETEVEIVERKGVGHPDSLADGIAESVSRALCREYIERFGRILHHNTDEVQIVAGESRPEFGGGEVIRPIYILVTGRATAYVGDEEIPVGTIALEAATEYLEETVRHLDTEKHVVIEPKIGHGSADLRDVFERGEEVPLANDTSFGVGYAPLTTTERLVYETERKINSPKLKKDLPEVGEDVKVMALREDNKIELTVAAAMVSQRIDDIDHYISVVEELRDRVADLAAEIASDHDVEVHVNTADDYERESVYLTVTGTSAEQGDDGSVGRGNRVNGLITPFRPMSMEAAAGKNPVNHVGKLYNVLAHQMAREIYEETSADEVYVRLLSQIGHPIDDPKAVDVHLVGDVSEEDERVARRVADELLENITELTERIVEGEIEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV93</accession>
    <name>Q8TV93_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ni,Fe-hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hyaD_1</name>
        <name evidence="2" type="ordered locus">MK1499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02712">
        <property type="protein sequence ID" value="AAM02712"/>
        <property type="gene ID" value="MK1499"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1499"/>
    <dbReference type="HOGENOM" id="CLU_1485892_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="181" mass="19640" checksum="75A5D05A8E18F056" modified="2002-06-01" version="1">MIGPLDPLRDYLRELLGEGFVLVGLGNPLAADDGFGHWVARRLSPLKTHKFKAVAAGVWLERLRLPNEPVVLVDTVRVDREPGTLVVVKEPRLGPDRGSHGPPPDELMDVRLLIGFVPVKSGFGPMSPEAKSVADAVVRVVRDVVLEGTEGPTDRGLRGSAALHRERQKSAQTTRGSVRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA8</accession>
    <name>Q8TVA8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaea-specific flavoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1484"/>
    <dbReference type="EnsemblBacteria" id="AAM02697">
        <property type="protein sequence ID" value="AAM02697"/>
        <property type="gene ID" value="MK1484"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1484"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1642"/>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="OMA" id="IVWCITG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901285">
        <property type="term" value="P:5,6,7,8-tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014073">
        <property type="entry name" value="DmrX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02700">
        <property type="entry name" value="flavo_MJ0208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="26980" checksum="FC9131292A050A41" modified="2002-06-01" version="1">MGLRIGWGITGAAHLLVETFKIMKEISREHRISAFLSEAGEEVVRMFGLWDDLREICPGGFYREVFTQSEEGASCPIIGRFALGKYDLLIVSPATANTVAKIAHGIADTLVTNAVAQAGKGDVPVWVVPCDYEEGKIRTITPYMVLRERCEGCGICVDACPRSAIDMVDGKAFIRLLRCVGCGKCAEACPEDAIHGGLEYEMRVRSVDAENVRKLERIEGIEVLRHPEEIPERLGELAGEGTTPDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC3</accession>
    <name>Q8TVC3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cacineurin superfamily phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02682">
        <property type="protein sequence ID" value="AAM02682"/>
        <property type="gene ID" value="MK1469"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1469"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1625"/>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="OMA" id="AVPGNCD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07392">
        <property type="entry name" value="MPP_PAE1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041733">
        <property type="entry name" value="PAE1087_MPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="5"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="206" mass="22144" checksum="AF7D9572B1A55BF2" modified="2002-06-01" version="1">MIGIATADFHGDVEKAEQVAEKAADEDADVIFVAGDVSDFNLEDPAQVAEEIVDVLKEHGQEIMAVPGNCDTPEVVRVLDTSGVSVHLKVKHIGRYDVVGMGGSNPTPFDTPLEFEEDVIESRLRELMNSTDEPVILLTHAPPKDTAVDKVEAGDHVGSEAIRKIVEEFQPKLHICAHIHEAAGEDELGNTRVINPGPGGTVVVEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE8</accession>
    <name>RL24E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24e</name>
        <name type="ordered locus">MK1444</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE8"/>
    <dbReference type="SMR" id="Q8TVE8"/>
    <dbReference type="STRING" id="190192.MK1444"/>
    <dbReference type="EnsemblBacteria" id="AAM02657">
        <property type="protein sequence ID" value="AAM02657"/>
        <property type="gene ID" value="MK1444"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1444"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1600"/>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="OMA" id="GTGKMYV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023438">
        <property type="entry name" value="Ribosomal_L24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_L24e-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_L24e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="PTHR10792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000136917" description="50S ribosomal protein L24e">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <sequence length="68" mass="8063" checksum="AC4D8BB3495C9AD3" modified="2002-06-01" version="1">MPDVRRCDFCGRIIEPGTGKMFVKNDGTILWFCSSKCERNMLKLGRDPKKVRWTEKHREFMAEQRGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH3</accession>
    <name>Q8TVH3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nitrogenase subunit NifH (ATPase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nifH</name>
        <name evidence="9" type="ordered locus">MK1416</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1416"/>
    <dbReference type="EnsemblBacteria" id="AAM02629">
        <property type="protein sequence ID" value="AAM02629"/>
        <property type="gene ID" value="MK1416"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1416"/>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="OMA" id="YVCDYYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM02629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="266" mass="29158" checksum="3FA297DD5D162F0C" modified="2002-06-01" version="1">MKRIAVYGKGGIGKSTIAANVAAALAEEGYRVMLVGCDPKADSTLTLAGRRIPTVMHEYRRKGEGLKLEDIIVEGDFGVLCVESGGPKPGVGCAGRGVLKALEMLTRMGAFEDVDVVIFDVLGDVVCGGFALPIRRGYADTVFVVTSSEPMSLYAANNICRGIAEYADRGGAKLGGVIHNRRSRDSDSRVVTEFCRRIRAELIYDLFYMEEVRKAESRYRTVIREFPDSDAAEAFRELAHRMLETEGVVPQPLEEEEVLRLAGVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN8</accession>
    <name>Q8TVN8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted cysteine protease of the transglutaminase-like superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02563">
        <property type="protein sequence ID" value="AAM02563"/>
        <property type="gene ID" value="MK1350"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1350"/>
    <dbReference type="HOGENOM" id="CLU_1113875_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="134"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="27831" checksum="07E2D22B64208657" modified="2002-06-01" version="1">MTRNSEVVDWLHSSNGPTWPLSNWVKAVKYIVYVAPERKEDPTPLKVVQVAESPNTLDCSTKDVPYWGEVTWGKVSERDFANAVQAMNGGTLESQLFKAAVRIAYWVASKIPARDYSTPGDMDWTEFTPLQVLKEYWRGWNCAGHAVLAVALMRAAMIPAKVVGGLLAGEGHAWVAFYNQDFRWVHVDPTGDAGNSYMQLYLAPRVITDDDVEAHSGYYCDDNATTIDDIEREWIPVAPIVVVAALMIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ0</accession>
    <name>Q8TVQ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1338</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02551">
        <property type="protein sequence ID" value="AAM02551"/>
        <property type="gene ID" value="MK1338"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1338"/>
    <dbReference type="HOGENOM" id="CLU_1615318_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="164" mass="17955" checksum="04621D4550EBB321" modified="2002-06-01" version="1">MFLVRVLLTDPVGPHLEAYGTVPPEAWSLLEVVLQSLEGRIDRLLGLELTPASVALESIAGDVGVVLLTSTDPEPVVRSVRRVFGSCDVEGVYREDVGEAREELEDMVPEDTIVSRELDVLGRDWCVLGAGTALLSGGKVPALEEIRPGEIRAWNSFSREWFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR0</accession>
    <name>Q8TVR0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02541">
        <property type="protein sequence ID" value="AAM02541"/>
        <property type="gene ID" value="MK1328"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1328"/>
    <dbReference type="HOGENOM" id="CLU_2550280_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="82" mass="9469" checksum="C4704F7D5256BA31" modified="2002-06-01" version="1">MGVVESTEVPIFRDQEGKARALILRYDPDGGVFVRYGEREDGSWEWTGFRLTAAEVALLKEFFNRIDPLHVQRLESGRRDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS1</accession>
    <name>Q8TVS1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02530">
        <property type="protein sequence ID" value="AAM02530"/>
        <property type="gene ID" value="MK1317"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1317"/>
    <dbReference type="HOGENOM" id="CLU_762100_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="363" mass="39787" checksum="D226DBA102DF7F7A" modified="2002-06-01" version="1">MELVLELSLGRFRAGDAIERTRTSVPAQTLFGAILGATLELLRGDGEDPDVVKEVLDALVGGLELTDAFPLDRGGEPLLPVPEHVRRELSDPERYGEVLRELAGDDVDTGTHGLVTEPERLPLSLFTDLCVEGSPTDPATLKRLREWLSGEHDPVVGSGRLTHASVPRTGDDTTPFTLDYASGRGIEGPTHVAFLRYDGKEPDYYDVEALLRAVLRYLRDAGLGGARSRGAGEVLEAGLREPEGEEKLLFSQRMRVEEGEPAITLSACAPEGDAEFYGRVERRGLHYARVGPFVTNYRVPRHYLAATGSYFPEWPGAENLRFEVPEGLLPGFLRGDPMYRGGYELVVYGRGFPVRVRALGDGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV3</accession>
    <name>Q8TVV3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotidyltransferase of the DNA polymerase beta family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02498">
        <property type="protein sequence ID" value="AAM02498"/>
        <property type="gene ID" value="MK1285"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1285"/>
    <dbReference type="HOGENOM" id="CLU_159724_0_0_2"/>
    <dbReference type="OMA" id="ERKYDIM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_2" evidence="1">
        <location>
            <begin position="32"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="124" mass="15008" checksum="DFF2C5EDF8070618" modified="2002-06-01" version="1">MSRGDVPDWYVKYLRRRYRLFLEYVRRWRYWVRRIAEVIRERVDPRAEVIVFGSVVKGRATGGSDVDVLVVTERARELRPHEVAGIVEEELDLPEDHPFEFHLTTPEGFETRWRRFLDEYERVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV8</accession>
    <name>Q8TVV8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02493">
        <property type="protein sequence ID" value="AAM02493"/>
        <property type="gene ID" value="MK1280"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1280"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1399"/>
    <dbReference type="HOGENOM" id="CLU_2893260_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="62" mass="7352" checksum="BFEED40C340AC619" modified="2002-06-01" version="1">MGIDYTTERVIIHRQRLPEILEDRIIEKYGLDKVQESDHHWRKVSEPALSVTRKLVPRTIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW06</accession>
    <name>Q8TW06_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Membrane subunit of an iron-regulated ABC-type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sufB</name>
        <name evidence="1" type="ordered locus">MK1232</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TW06"/>
    <dbReference type="EnsemblBacteria" id="AAM02445">
        <property type="protein sequence ID" value="AAM02445"/>
        <property type="gene ID" value="MK1232"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1232"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1335"/>
    <dbReference type="HOGENOM" id="CLU_894122_0_0_2"/>
    <dbReference type="OMA" id="PVHLCFG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="316" mass="34464" checksum="C04E65EACBEBA7CF" modified="2002-06-01" version="1">MPDVRGWPGVRGGVSPEEVFKPTGYHAGPDTPRIVIIENKVVNVQGAEGLELNAEEEDDTVVAELVVKEGYEFDEPIHMCVGVPWPEGVQRIVTRLIVEPEAKIQLMSHCSFPRARDVVHEMEAEFEIGEGADVKVTDVHYHGEGGVRLKAKYDVSVKPEASFVTEFRLTEGRVGELEWEMGARVEERATMEGVARLRAVGEDRVRAVESVRLTGEDARTLLDFKAAAIDGAFVELVGEISGEADGARGHVECSEIIKGGGKVVSVPKLRVVHPGARLTHEAALGTVEKKEVETLMSRGLSEEEAVELLVNAMLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW44</accession>
    <name>Q8TW44_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02405">
        <property type="protein sequence ID" value="AAM02405"/>
        <property type="gene ID" value="MK1192"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1192"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1293"/>
    <dbReference type="HOGENOM" id="CLU_037423_2_0_2"/>
    <dbReference type="OMA" id="HGLFWRG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="248" mass="27407" checksum="244158BB2A55D5D7" modified="2002-06-01" version="1">MAMVREVIDFVEELAPPDLAEDWDNPGIQVCPPGGLDRKAERVLVALDATHALERAGDADLVLTHHPLLFRPPRRIGGRWYRVLRAVLEADAVFYAAHTNLDRAEGGVADTLARRLNLRVEREACDGFGRLCEVPGSEEELLNALRNLSPLTTVYGDWEGVSRVLVVPGAAPDGLVMECLRCGVDAVIAGEIKYHTRAELLEEGIAVVELGHEYSELPGVQELARRVREEFRDLNVEVVPPPDITIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW67</accession>
    <name>Q8TW67_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02382">
        <property type="protein sequence ID" value="AAM02382"/>
        <property type="gene ID" value="MK1169"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1169"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1256"/>
    <dbReference type="HOGENOM" id="CLU_971854_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="286" mass="32812" checksum="48C4BF69A738AFDD" modified="2002-06-01" version="1">MTSLESELREALRERRLRKKWAVVAVVYLAWKMYGKHVSAGDIRDVLESVGYEISEHAVHAYMSGLRGLIGRRKAGRRTLYTVRNEEEMRRRLRDALRGSVGRISSEEKEALIESILGGDRGRRDPEVENLVLIWRRPTAEYRRRIASSVHPGSRLREIPGVFGTFEGIWGFGSGERVGWESLGEGDVLLFRDEKGEDRYRYCGVIASKCNDPKVSAILWGDDRWERLVTLAEVRLVSLPASELNELLGYRAGFRPRGAMRVDPDRVSKLVERYGGVLEFLREYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW76</accession>
    <name>Q8TW76_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-26 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02373">
        <property type="protein sequence ID" value="AAM02373"/>
        <property type="gene ID" value="MK1160"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1160"/>
    <dbReference type="HOGENOM" id="CLU_965091_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="288" mass="32389" checksum="D40544407C96F94B" modified="2002-06-01" version="1">MGIRGSRGVVSEFEGSLKREPDRWWFTRFLHEHPMLLSTSLALLTLPPVLTAGRTVWELSDRPHDPAALLVTTSATLAPALAVYSALMAYYTLRARYLIRAPVVTYPHHPAYLPLLYWLPSSRLLTHWGEPKVRLGFVVLYTTVVLLPTLSLLLTWICRVRILWCWRGDIDEGTLARLLERLPRDYTYAPGCVFPALVAISALPGAFGYLLLKGTWSGWYVLAIPASMIVPMTVLTYAVIRKMSRSPVEELTDEWLGEGNAPWRLDLQALAILATFATWTLVDVGGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW84</accession>
    <name>Q8TW84_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-9 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02365">
        <property type="protein sequence ID" value="AAM02365"/>
        <property type="gene ID" value="MK1152"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1152"/>
    <dbReference type="HOGENOM" id="CLU_932595_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="298" mass="33475" checksum="A3B86C8EBE788844" modified="2002-06-01" version="1">MIVVITAAISLQPKASHATLAATEFLILILALSAVNYVLLPLLGFGAVWVFRVGKRLYLVSLGYCEGETILEPGVEVELSGFQVLAEFPLESEYHGRLWIRIGYFPPGIHWVELPERGFFFLRTRGVCALRDPYLPRDDEGALEMARFLEKLSMVYAAVCLEEGGPNPGRARKIMKHVKNNSRWGTTVRYFGVKVPLGRFTTSQSTTVTVAVLVALSLVSALARGEPLGPMGAPILVLSWLLACVLLALPVAWRNRRAVKTGRWYRVIFERHRVEEAIDKARDWNWFPDLERVDPLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI9</accession>
    <name>Q8TWI9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1045</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02258">
        <property type="protein sequence ID" value="AAM02258"/>
        <property type="gene ID" value="MK1045"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1045"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1098"/>
    <dbReference type="HOGENOM" id="CLU_539290_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="505" mass="57512" checksum="F55169DD4F60E3AF" modified="2002-06-01" version="1">MRFPALVIGLLVALGPTAASESPKVAVVMPYHGSYDPHWIYMAHELPEKVQEILSVTYDEYNIQVYPGSFETYDVTKVRLQGGKEYVIHQEEVNTGEEVLKTELGHALKEAEEFTNSGRDGLIVVLPAFVNRGGQHQCVEIPEVIDIILGDKVPKYSVHYEPFGITNWDRQNDIGIVPELLFRNIQTEAEKTLTGPNGQGDPEDVFSAYKDKRLLDFLVDHKDEVGLLEIMFGTLRVGYWDDFKANAMYVLRGVAERLGNLLGTTIPVGEVDWALENPFVREGKAYFFAFQNPEIGPENWEKSRWEVRYEQFQKLMQSLCRQGVRYVVAFPYITVTGITDRKLFEGNPEHGPEGDEPEAYDYPEAILRHFGAELVAETVADVRKYTYVQEGQEKTRTVYINARIYRIPKEKLGTDADMYVIYVRRGLLDAPNATEKVARVYANYIATHLPTWLMEYEAVKGMKEGFEGEINSLRKDVKSLKKRKVAVYPAVIALALAMIPPLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM6</accession>
    <name>Q8TWM6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized archaeal membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02220">
        <property type="protein sequence ID" value="AAM02220"/>
        <property type="gene ID" value="MK1007"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1007"/>
    <dbReference type="HOGENOM" id="CLU_149108_2_0_2"/>
    <dbReference type="OMA" id="LMWSAAK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="88" mass="9216" checksum="FD0A6FD68EFC2E79" modified="2002-06-01" version="1">MIALRVEDVLKAGVLITFVGIVLTALAILLLAIKNASGRGEWGGVILIGPVPIVVGSSPKMALIVAVLALAMMLIMLFLMWSAAKGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP4</accession>
    <name>Q8TWP4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0988</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02201">
        <property type="protein sequence ID" value="AAM02201"/>
        <property type="gene ID" value="MK0988"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0988"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1035"/>
    <dbReference type="HOGENOM" id="CLU_1529238_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="66"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="175" mass="19419" checksum="2EBE2D452016C4E8" modified="2002-06-01" version="1">MAGAGVSASREDIALRLRRVSRVWGAALYVGSVLLIVLIAVLGLGALGQYSYQAQVLLFGNYRGQLAEIDEKYQVLKTEFDQANHKLQALRDLYPISSADLHQVEKIRAKASLELQSLKLKKKDTEFAVYVLKDPATSDLKLKECMAELRRAEAAVKAFVRAVKGLEDKYAGRVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWQ2</accession>
    <name>Q8TWQ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02193">
        <property type="protein sequence ID" value="AAM02193"/>
        <property type="gene ID" value="MK0980"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0980"/>
    <dbReference type="HOGENOM" id="CLU_043914_0_0_2"/>
    <dbReference type="OMA" id="FGCFAGR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13186">
        <property type="entry name" value="SPASM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="87"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="403" mass="45240" checksum="B33909AC64DECE94" modified="2002-06-01" version="1">MTAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVFSLYLPPFPSRAFTRLLRARLKSEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELSTEDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDDDRAIATAFTSGYTLKDRVKELKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVETGKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSGPRVSAMSYVNSEHGAGCFAGYVQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSHPEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEGDGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR4</accession>
    <name>Q8TWR4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2007-08" db="PDB data bank">
            <title>Crystal structure of an uncharacterized conserved protein from Methanopyrus kandleri.</title>
            <authorList>
                <person name="Bonanno J.B."/>
                <person name="Zhang A."/>
                <person name="Bain K.T."/>
                <person name="Adams J."/>
                <person name="Ozyurt S."/>
                <person name="Smith D."/>
                <person name="Wasserman S."/>
                <person name="Sauder J.M."/>
                <person name="Burley S.K."/>
                <person name="Almo S.C."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS) OF 2-114</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="2QYA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.17 A"/>
        <property type="chains" value="A/B/C/D=2-114"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QYA"/>
    <dbReference type="SMR" id="Q8TWR4"/>
    <dbReference type="STRING" id="190192.MK0968"/>
    <dbReference type="EnsemblBacteria" id="AAM02181">
        <property type="protein sequence ID" value="AAM02181"/>
        <property type="gene ID" value="MK0968"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0968"/>
    <dbReference type="HOGENOM" id="CLU_174522_0_0_2"/>
    <dbReference type="EvolutionaryTrace" id="Q8TWR4"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007355">
        <property type="entry name" value="DUF424"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04242">
        <property type="entry name" value="DUF424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="3" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2QYA"/>
        </source>
    </evidence>
    <sequence length="114" mass="12488" checksum="3174EC3AE408E340" modified="2002-06-01" version="1">MARVLLNIHGTGDTVVLALCDEDLLGVELKYKGRTLHISEPFYSGKSLEPDRAAKKIREAVQEYEDEKTVAINALGELACSVVVDAGLAREDEIGELGGVPHVQIYILPREPFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-13" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU7</accession>
    <name>RSMA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">MK0934</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWU7"/>
    <dbReference type="SMR" id="Q8TWU7"/>
    <dbReference type="STRING" id="190192.MK0934"/>
    <dbReference type="EnsemblBacteria" id="AAM02147">
        <property type="protein sequence ID" value="AAM02147"/>
        <property type="gene ID" value="MK0934"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0934"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.977"/>
    <dbReference type="HOGENOM" id="CLU_041220_0_2_2"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000101657" description="Probable ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <sequence length="278" mass="31520" checksum="C1C8DC1A2994EB10" modified="2002-06-01" version="1">MRSVEYLAYLRSKYGIRPRRRLGQHFMVDDNILEFMVEAAEVREDDIVLEIGPGPGLLTRYLMTRAGQVIAVELDGRMVEILKRELGEAPNLEIVRADFLEYDVPDDVNKVVANIPYNISSPITFKLLELDIDVAVLTYQREFAERMVAEPGSKKYSRLTVMVNLLADVELLRGVPRRAFIPPPRVGSSVVRLTPKSEEERPDVDPDTLESVCRALFQHKNKTVRNALLLSAHEWATDREQAREVLEELPEDLLSERPLHLPPERVAELAAAIESALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWV1</accession>
    <name>Q8TWV1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420-reducing hydrogenase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0930"/>
    <dbReference type="EnsemblBacteria" id="AAM02143">
        <property type="protein sequence ID" value="AAM02143"/>
        <property type="gene ID" value="MK0930"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0930"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.972"/>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="CNAENVH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="185"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="215"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="27679" checksum="80381CF79A0A2161" modified="2002-06-01" version="1">MVRWWRRLSRAKTKSTPRSSCDVAYVQLAGCCGCLVSLTDTYERLLDILDSIELVYCQTLMDEREIPECDVAVVEGAVCLNDEHMLEEVEELEEHADTIVALGACASTGNFMRLSRGNQQAAPTHEAFVPLTEVADVDYAVPGCPPAPEAIKRFLTLLLEGKEELLEPFAKLAEGKTEYCGCDLMYHVVNKSMCMGCGTCAAACPTRAIEIVDGRPVVNENRCIKCGACFTQCPRTIWPGDEAIKELIMGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW8</accession>
    <name>Q8TWW8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02126">
        <property type="protein sequence ID" value="AAM02126"/>
        <property type="gene ID" value="MK0913"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0913"/>
    <dbReference type="HOGENOM" id="CLU_539289_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="505" mass="54506" checksum="7D831D5483A67C4A" modified="2002-06-01" version="1">MGAYLSSHLDHLRGHSLEMAVLAILTVTALVIIPEDVPTIPYVSLISISSIKAAHDSLRSGELPQWNPYLGGGFPLAPTPYYLLHALGRTGLIIAILLLSVLVLLLASNVNNMLRVVITLPAVLLSLIIPSPLPLILASYALGFIGNNPLLRTVGSIGIPDVALSIELGIRNPSLPVLLASSACLVDCLVATFGAVPLLTSRPGPIPLLLILLITVVAILPALMALAMLGRIYPDYRWKFSGITAIILFGVQTYLHELPTALATPIILFSLLVLKNFNTHLDRRISVRLNRVSKPLRFLTALMLVLFFIVICSVLATPRFAVVGYKVVGSRTGMEYIISSDPWFSECVKRVLSGHPIWNTPLPTDPHMGPASLLGYELGDPRAVPPLYPTKQLEVQPRVVAPLISSPSSWAETSLHALQLAEAVASRGASVGEPRLNTPTEIRGYGLVGQVYDLWWKGGTHRGLLLQVNGRAVYERPSAWPSVVLTLMSIAVAAIETAKRKSDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX6</accession>
    <name>Q8TWX6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02118">
        <property type="protein sequence ID" value="AAM02118"/>
        <property type="gene ID" value="MK0905"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0905"/>
    <dbReference type="HOGENOM" id="CLU_144581_0_0_2"/>
    <dbReference type="OMA" id="IFISEMD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020380">
        <property type="entry name" value="Uncharacterised_MJ1658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17393">
        <property type="entry name" value="DUF5402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="120" mass="13143" checksum="93387F961884AD11" modified="2002-06-01" version="1">MSKKLLRRCAAQLKRVQDALGDDAVVSEVDLFVARCGCVGVVFMVRGVELRDISDEVLDGLEEAGKALGVRPDVVYARVVPGTQVVIDLAVRTLCDTCRREFSGEEPRPDLKVLRGATER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWX9</accession>
    <name>Q8TWX9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02115">
        <property type="protein sequence ID" value="AAM02115"/>
        <property type="gene ID" value="MK0902"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0902"/>
    <dbReference type="HOGENOM" id="CLU_504008_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="540" mass="60838" checksum="97C088ED303C6F34" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY1</accession>
    <name>SYA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">MK0900</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02113" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02113.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWY1"/>
    <dbReference type="SMR" id="Q8TWY1"/>
    <dbReference type="STRING" id="190192.MK0900"/>
    <dbReference type="EnsemblBacteria" id="AAM02113">
        <property type="protein sequence ID" value="AAM02113"/>
        <property type="gene ID" value="MK0900"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0900"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.942"/>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075265" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="882"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="609"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="915" mass="103908" checksum="6DD88BA1939CB4EE" modified="2002-08-02" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ6</accession>
    <name>Q8TWZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted transcriptional regulator consisting of an HTH domain fused to a Zn-ribbon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0885"/>
    <dbReference type="EnsemblBacteria" id="AAM02098">
        <property type="protein sequence ID" value="AAM02098"/>
        <property type="gene ID" value="MK0885"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0885"/>
    <dbReference type="HOGENOM" id="CLU_162441_0_0_2"/>
    <dbReference type="OMA" id="LVAPPEC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038767">
        <property type="entry name" value="Transcriptional_reg_pdt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40663">
        <property type="entry name" value="PTHR40663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="98" mass="11621" checksum="B1E7F0F3F5D21633" modified="2002-06-01" version="1">MRWETRREKIVSLLTESREPLTIEEIAYMVGEDDKSKIIEDLEHIAKTLRREGKTLLMEPARCNKCGYVFKSLRVKPPSRCPKCKSEWIQPPRFTIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ7</accession>
    <name>Q8TWZ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0884"/>
    <dbReference type="EnsemblBacteria" id="AAM02097">
        <property type="protein sequence ID" value="AAM02097"/>
        <property type="gene ID" value="MK0884"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0884"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.926"/>
    <dbReference type="HOGENOM" id="CLU_104481_0_0_2"/>
    <dbReference type="OMA" id="ATNTGVW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK C-terminal" evidence="1">
        <location>
            <begin position="108"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="21809" checksum="5B1EBA30BC17FE63" modified="2002-06-01" version="1">MEKIEEEVKKRTVVELLTEMKNLAQLSVDLAYSVLLFGSPELAEEVRRIERRVDELSMVLKAKLALAIRDLEDVRHLLPIMELTQSMEVITDAANDIAETVAMGAEPHPIVQKAIAESEEKIRLVRVEEGSELDGKTLGELRLASETGMHVIAIRRGSTWIIGPDKDAKILGGDVLIVRGRDEGYRKLKKMASGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX05</accession>
    <name>Q8TX05_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Triphosphoribosyl-dephospho-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">citG_2</name>
        <name evidence="2" type="ordered locus">MK0876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0876"/>
    <dbReference type="EnsemblBacteria" id="AAM02089">
        <property type="protein sequence ID" value="AAM02089"/>
        <property type="gene ID" value="MK0876"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0876"/>
    <dbReference type="HOGENOM" id="CLU_056179_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30201">
        <property type="entry name" value="PTHR30201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="308" mass="33449" checksum="42424E6709FF0134" modified="2002-06-01" version="1">MVGTVRELPTLFCESVALEVLSGPKPGLVDPISPSSHEDMGPREFLACLPELRRCLEEALELGIDGEPVDELIRSLPAWNVDVITASGGPNSVRGIVFLGSVYCYSAGLENDPFPFGRIRVVGRRCTEALWNRSETKCGRIRIKERLAGVFGEVASGMATARGVSLPVLRATLRAGRPLEEAVIHSVLACMSTLEDAGIPRKLRNWVKRRASEVLRAGGPFTERGRAELHKFVHECAERGVSPGASADVTVVGLVLLLARWGRDIVSPYRDPPRRWSGGAVERNSQASDTVGRNPGGRVRGRREVLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX14</accession>
    <name>Y866_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MK0866</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0866</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX14"/>
    <dbReference type="SMR" id="Q8TX14"/>
    <dbReference type="STRING" id="190192.MK0866"/>
    <dbReference type="EnsemblBacteria" id="AAM02079">
        <property type="protein sequence ID" value="AAM02079"/>
        <property type="gene ID" value="MK0866"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0866"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.908"/>
    <dbReference type="HOGENOM" id="CLU_056125_0_0_2"/>
    <dbReference type="OMA" id="GHKHHIY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="CHP00300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00300">
        <property type="entry name" value="TIGR00300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107366" description="Uncharacterized protein MK0866">
        <location>
            <begin position="1"/>
            <end position="412"/>
        </location>
    </feature>
    <sequence length="412" mass="45747" checksum="B08DC8A34DD2A0DC" modified="2002-06-01" version="1">MTDREEVVELRGHIIDSLIFSRVLDTIMEMGGDFEILEFKVGKRKTDPSFAKILVKGKDPEHLREIVSELRKYGAVPVHTQEVRLEPAPADGVCPRGFYTTTNHRTFVLFDGEWIEVEDIEMDCAIVVYPEERRAVAKPIREVREGELVVVGDRGVRVKPPERPRGRTGIFGFMESEVSPEKPTPTLIRRIAEELEWHRKNGKIVVVVGPAVIHAGARDDLAWMIREGYVDVLFAGNAVATHDVEASLFGTSLGVDLETGEPVKGGHSHHLYAINEIRRVGGLREAVEKGILKDGIMYECIVNDVPYVLAGSIRDDGPIPDVITDVMEAQAEMRRHLKGATLVLMMATMLHSIATGNLLPSWVKTICVDINPAVVTKLMDRGTAQALGIVSDVGVFLPELVKELKRVRDDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX71</accession>
    <name>Q8TX71_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">apaH</name>
        <name evidence="3" type="ordered locus">MK0804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0804"/>
    <dbReference type="EnsemblBacteria" id="AAM02018">
        <property type="protein sequence ID" value="AAM02018"/>
        <property type="gene ID" value="MK0804"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0804"/>
    <dbReference type="HOGENOM" id="CLU_074761_0_1_2"/>
    <dbReference type="OMA" id="DEYLFPW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos_2" evidence="2">
        <location>
            <begin position="4"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="232" mass="26230" checksum="7972620340B2A366" modified="2002-06-01" version="1">MTVEIAVISDVHSNLEALKRVLREVRSADMLVCCGDIVGYGPRPVECVDIVREWCDRCVMGNHDYGVVTGDVAYFNIAARIAVEWTRRQLDEDRREFLAKLPKTERFEVEGVSIFLVHGSPRDPIWEYVFPHTPRQLLEKLVDKAGTDVLIMGHTHVPMYDEVNGSYVLNPGSVGQPRDGDPRAAFGILEVSNGRIVSWDVHRVAYNVDRTAREIEERGLPEELGARLYRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX75</accession>
    <name>Q8TX75_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator containing a wHTH DNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0800"/>
    <dbReference type="EnsemblBacteria" id="AAM02014">
        <property type="protein sequence ID" value="AAM02014"/>
        <property type="gene ID" value="MK0800"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0800"/>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="OMA" id="EDLMRCI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="127" mass="14575" checksum="0553240C10D21CAB" modified="2002-06-01" version="1">MLADVPFVTVRRLNVTVRDVLRCVLGLRDVEVDTYFALLERGEATVYDLAEELDRDRTTVQKALKSLVYAGLVTRRKETRPRGGFVYVYKAVPFEEARKIVLRALDEWYEAVKDALERADVPRSGPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX94</accession>
    <name>LEUD1_METKA</name>
    <protein>
        <recommendedName>
            <fullName>3-isopropylmalate dehydratase small subunit 1</fullName>
            <ecNumber>4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-IPM isomerase 1</fullName>
            <shortName>IPMI 1</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Isopropylmalate isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuD1</name>
        <name type="synonym">leuD_1</name>
        <name type="ordered locus">MK0781</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX94"/>
    <dbReference type="SMR" id="Q8TX94"/>
    <dbReference type="STRING" id="190192.MK0781"/>
    <dbReference type="EnsemblBacteria" id="AAM01995">
        <property type="protein sequence ID" value="AAM01995"/>
        <property type="gene ID" value="MK0781"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0781"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.822"/>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="OMA" id="GLPIIEC"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141938" description="3-isopropylmalate dehydratase small subunit 1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="18686" checksum="E329CD3D366367F6" modified="2002-06-01" version="1">MRDVIRGRAWVFGDDIDTDQIIPGRYLTTQDPEELAKHVMEGADPEFPEKVREGDVIVAGKNFGCGSSREHAPIALKAAGIACVVTRSFARIFYRNAINLGLPLVVCPGVDDAFEDGQGIEVNLREGYVRNLDTGEELEAKPLPDFMMRILEAGGLVELIKREGPRAFEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB6</accession>
    <name>SYR_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">MK0758</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXB6"/>
    <dbReference type="SMR" id="Q8TXB6"/>
    <dbReference type="STRING" id="190192.MK0758"/>
    <dbReference type="EnsemblBacteria" id="AAM01972">
        <property type="protein sequence ID" value="AAM01972"/>
        <property type="gene ID" value="MK0758"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0758"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.798"/>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151647" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="592" mass="67767" checksum="E395C45BCD470C52" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC4</accession>
    <name>Q8TXC4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea, Zn-ribbon domain containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0750"/>
    <dbReference type="EnsemblBacteria" id="AAM01964">
        <property type="protein sequence ID" value="AAM01964"/>
        <property type="gene ID" value="MK0750"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0750"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.790"/>
    <dbReference type="HOGENOM" id="CLU_056316_0_0_2"/>
    <dbReference type="OMA" id="GIRVMCG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="415" mass="44236" checksum="DB1FD7A55B905C06" modified="2002-06-01" version="1">MIPLIALLALVSGASATFNMIVITDPSGKDPNGAAAASMSFAPNMFQSTFLVSKKLHVAVLAGGLSKGTARLEAILACIRALELGEDIESAVRAGISRSPTDRLLVGGPGKGVAVGGSYDIAVVIVKGNKIIIKQYHSSGGPNIVRIPKNVKCAVIHLRNTPGNPMYGTATKVRIEAAIMAGRMIRDGLPATEIVTRIMGYVAKKSGEKYGGGVVNITAGLSTGDTFVPPKLNARGIPMDTAYRKVCPKCGWSVAYPAASKYSRCPVCGSSLRTEYAWQVARDMITVRKDQPIVRVYGIKSVYDKVSIVETVQTLVQAGKKQPEDIARAIDSDIDNNTLLGYDYILPGDVKVEPEANLITIYMRPLPEGYKKPPLKTPISPETLHSLGLISSAVGVALIVLGGIRELVNRRLRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG2</accession>
    <name>Q8TXG2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphopantothenate synthetase</fullName>
            <shortName evidence="1">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4-phosphopantoate + ATP + beta-alanine = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0712"/>
    <dbReference type="EnsemblBacteria" id="AAM01926">
        <property type="protein sequence ID" value="AAM01926"/>
        <property type="gene ID" value="MK0712"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0712"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.753"/>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="OMA" id="PKSHPRY"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="PTHR40695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="187"/>
            <end position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="254" mass="28122" checksum="2E3AC36970D38B05" modified="2002-06-01" version="1">MKIPPDHPRAEALKIRERLVRGFEDGYVVPQGLIAQGRGEVFDYLIGEKTIPPAREAIKAAAAAFKLAEHPVISVNGNTAALCPEDLVELSEVTGAPLEVNLFHRTEDRVEKIAETLREAGADRVLGADPEHLVRLPNLSSHRAVVDDRGIYRADVVLVPLEDGDRTRALKELGKTVVAIDLNPLSRTARDADITIVDNVVRCVRKITREYERMDPDRAKGVLREYDNGRVLARVLDHIRERLRVLADEAAGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG8</accession>
    <name>Q8TXG8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK0706</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0706"/>
    <dbReference type="EnsemblBacteria" id="AAM01920">
        <property type="protein sequence ID" value="AAM01920"/>
        <property type="gene ID" value="MK0706"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0706"/>
    <dbReference type="HOGENOM" id="CLU_764224_0_0_2"/>
    <dbReference type="OMA" id="PYVLRHM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="192"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="362" mass="40006" checksum="9F65B03EB97196EF" modified="2002-06-01" version="1">MSLPSFSGLAEKLRESTSRMLGGRLESSLTAKVRAARHAVRGEQGEDVEKIKRIVLGIEEEGERDRTAEEINERLRRLCRERANVLETLIEMVQSLSPTLGNRFRGLAPDREVLQKAGLRISPAAFATFMIISGLMGVVLTSVPAALFPLPLPLKVLGPIMSFLLGMVVPRMMVTILIRRREGEIARQLPYAIRQMATEVSAGLSLIESMKSISESDYGALSEEFERVIREINSGTPINVALQRMANRWNVDGLRTMVRFITQAMESGANIAKTLMTLADEIAHELRQRYREYGHKLQALAFPYIMLTLVIPTLVTVAMLLAANLSGAFLVPPPLFGPMIAGMVGVMAGIFLFIFKSAEPKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI5</accession>
    <name>RPO5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo5</name>
        <name evidence="2" type="synonym">rpb5</name>
        <name evidence="1" type="synonym">rpoH</name>
        <name type="ordered locus">MK0688</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXI5"/>
    <dbReference type="SMR" id="Q8TXI5"/>
    <dbReference type="STRING" id="190192.MK0688"/>
    <dbReference type="EnsemblBacteria" id="AAM01903">
        <property type="protein sequence ID" value="AAM01903"/>
        <property type="gene ID" value="MK0688"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0688"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.727"/>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="OMA" id="PKIYHDD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="SSF55287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146093" description="DNA-directed RNA polymerase subunit Rpo5">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="11930014"/>
        </source>
    </evidence>
    <sequence length="92" mass="10282" checksum="E39A507AC03E6004" modified="2002-06-01" version="1">MAERLDPDVVLNHVLVPKHEVIDDEEEIEKILEELGVEKDDLPRIHTNDPVVVALSEKLGKRIKPGSLVKIVRDSPTAGKTVVYRVVTNPPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ2</accession>
    <name>RS12_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps12</name>
        <name type="ordered locus">MK0681</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ2"/>
    <dbReference type="SMR" id="Q8TXJ2"/>
    <dbReference type="STRING" id="190192.MK0681"/>
    <dbReference type="EnsemblBacteria" id="AAM01896">
        <property type="protein sequence ID" value="AAM01896"/>
        <property type="gene ID" value="MK0681"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0681"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.720"/>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_S12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_S12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_S23_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000146370" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <sequence length="147" mass="16433" checksum="88C70EAD342FFB65" modified="2002-06-01" version="1">MPGKKSPAGEFAARKLREKRKKFRWKDERYKRRMLKLDEKADPLEGAPQARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKQVTAFCPGDGAIDYIDEHDEVVIEGIGGPKGRAKGDIPGVRYKVVKVNDVALSELLKGKIEKPMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ7</accession>
    <name>TRUD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD</name>
        <name type="ordered locus">MK0676</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ7"/>
    <dbReference type="SMR" id="Q8TXJ7"/>
    <dbReference type="STRING" id="190192.MK0676"/>
    <dbReference type="EnsemblBacteria" id="AAM01891">
        <property type="protein sequence ID" value="AAM01891"/>
        <property type="gene ID" value="MK0676"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0676"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.715"/>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="OMA" id="GFPNYFD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000152542" description="Probable tRNA pseudouridine synthase D">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="162"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <sequence length="400" mass="46488" checksum="1B1E8E18AD139423" modified="2002-06-01" version="1">MEKPSRNTYIPLTIVGECPECREYHRGRECPECGQDLRRPRLRPQFGGRGPHTLFYLEKYDWDTMKAVRRIAQALRKHHRHFGIAGMKDKRAVTSQRVTVRGVPPGVLARLRIRDLKIVPMGRARRKLRPGDLWGNRFVITVRGAKVRRLPEALRTVRELGGVPNYYGLQRFGSRRPVTHVVGKYVVLEDWEKAVKTFLTLEYPRESPEALEARRWLKEHWGEFKEALRRFPKFLDYERHILEHLARHPHDYINAFRRLPMWIRRMFVHAYQSYLFNRILSERIARGLPVHRPVEGDVTQDGLPTVPLPGFRTELSDGPQGEIEREVLEEEGVRLEDFEIKSMPELSAGGDRKPALLRVYGLRAEAIGDDTVRFTFSLPRGGYATTVLRELLGSEGVYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK2</accession>
    <name>Q8TXK2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted NADH-flavin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0670"/>
    <dbReference type="EnsemblBacteria" id="AAM01885">
        <property type="protein sequence ID" value="AAM01885"/>
        <property type="gene ID" value="MK0670"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0670"/>
    <dbReference type="HOGENOM" id="CLU_120467_2_1_2"/>
    <dbReference type="OMA" id="VNSDFAT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="137" mass="14361" checksum="0FDBCC1477A98CAD" modified="2002-06-01" version="1">MKAETYALLAALLWGLAPVIEKVGLRGMDPMTATLIRSLAAVAFLAVVCLAVGRSQVGGLKYVGYMIVGGILAGGLGLYLYYLALSSGQASRIVPLSSTYPLFATLFSILALRERPSVETVVGCILIVIGAVLVSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK8</accession>
    <name>Q8TXK8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0664"/>
    <dbReference type="EnsemblBacteria" id="AAM01879">
        <property type="protein sequence ID" value="AAM01879"/>
        <property type="gene ID" value="MK0664"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0664"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.702"/>
    <dbReference type="HOGENOM" id="CLU_475411_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="573" mass="64580" checksum="36696B1C717F4873" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP1</accession>
    <name>Y620_METKA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0425 pyridoxal phosphate-dependent protein MK0620</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0620</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="2">Despite a certain similarity to selA, this is not selA (see AC Q57622).</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXP1"/>
    <dbReference type="SMR" id="Q8TXP1"/>
    <dbReference type="STRING" id="190192.MK0620"/>
    <dbReference type="EnsemblBacteria" id="AAM01835">
        <property type="protein sequence ID" value="AAM01835"/>
        <property type="gene ID" value="MK0620"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0620"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.658"/>
    <dbReference type="HOGENOM" id="CLU_055443_0_0_2"/>
    <dbReference type="OMA" id="RIDFCSK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020033">
        <property type="entry name" value="PyrdxlP-dep_transferase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03576">
        <property type="entry name" value="pyridox_MJ0158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000285291" description="UPF0425 pyridoxal phosphate-dependent protein MK0620">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="346" mass="38637" checksum="AC32C8DBB3DC229D" modified="2002-06-01" version="1">MRDIGSQPAAGVRSMKVRDRALEIIRERISRGDPLYDFTGLASRPKVDAASDELRTYLSEALILPEIRELCREHFDAPEYEVLVAPRTTAAVIATILALEPRSVLHVLPEDGEAHPCVEEGCRLAGAEYEEVEESEIPDLDGFDLVVVTGCDVRWNVVSEDTLREVASAEAVTLLDDASGDRVRRLHGQKPGPRYGFDLVVTSCDKLMDGPRAGVLIGRDDLVEGVRRVCEGYGWTVDGPTLAAVKRAFEEFELSSLEARLKELERVYERLKDELDVERTGAGLVFHGVEREVEIGLGLLRRYGIMTITALGYERVDRTLRFNLLTEDAERFGYDRFVEVVKGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP2</accession>
    <name>Q8TXP2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transcription initiation factor IIB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sua7_1</name>
        <name evidence="10" type="ordered locus">MK0619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0619"/>
    <dbReference type="EnsemblBacteria" id="AAM01834">
        <property type="protein sequence ID" value="AAM01834"/>
        <property type="gene ID" value="MK0619"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0619"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.656"/>
    <dbReference type="HOGENOM" id="CLU_043736_0_1_2"/>
    <dbReference type="OMA" id="MATACLY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="PTHR11618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="SSF47954"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="9">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="261"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="341" mass="39423" checksum="12108E87DFFD8450" modified="2002-06-01" version="1">MVYTESEHRVCPKCGSTNLERDEKHGELVCRDCGTVVDYLIDYGPEWRAFNADQRAQRERTGAPISERHPHKLGDTIIDDRDTRGRRLVDWRFRRLKRWDRWISNDPVSRNIRSAVELIERVCSQANIPRSIVDEAIRIYRKAVEKDLVRGRSIENTAAAALFMACKKRKHPRTIKEIAKLFGITPKDINRTHRVLLRHLNERMPAPDPKQYLSRFATELGVSEDVEMLAREILEKAEKKGLTVSRNPAGLAGAALYLAGLLKAKEYIEEYREKMKEAESEEEKEKLERELNEKLRTCRRTQPEVAKISGVTEVTIRNRYKELAEELGLEVPDPTKIDVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ5</accession>
    <name>Q8TXQ5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01820">
        <property type="protein sequence ID" value="AAM01820"/>
        <property type="gene ID" value="MK0605"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0605"/>
    <dbReference type="HOGENOM" id="CLU_040997_0_0_2"/>
    <dbReference type="OMA" id="CGHVSKS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="OrfB_Zn_ribbon" evidence="4">
        <location>
            <begin position="374"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="474" mass="55033" checksum="C24446DBB05B5A91" modified="2002-06-01" version="1">MPLRSDYRYRSITLKIVGPEGYRRDLIFLSDRCTAVYNELNYLRRRFFFKGKLDAVAVSRSELTFRRIYREYGPILGTQTVVAIGEKNTEAWRSYFKQIKEVKSNKRRTICQPPRYWKGPLGREPRFVFLASQIRRFEYDPLEHRGVIEILGLGDVVDNLPNVGFKRGRGRGVRLEFVGWFPWHPNRVKEPKRADKIILNGDDGKEFFGRSEIVYSWADNAHYLTLNMKVNKGESWLVSRLTPNGRVRPIPRTGNNCENAAAVDIGRRIFAAVYFSDGKKPVLVRWGDAWSRWSYWRKIKAPKEQRRLNKNGANFSHRLKQYYVKAHRAIMDAIRHLVSKLVQILLSREVTLLVIEDLKGLRQKLHGELAYWAYKRIIREIKRKCEEYGIEVIEASPAYTSVTCPMCRSKCKRNGGLVICSKCGKTMNADIVRAYNLLTRATDDPPEPELEYLTGSNCTTVLPSNHKPPITPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ6</accession>
    <name>Q8TXQ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">tRNA-dihydrouridine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0604"/>
    <dbReference type="EnsemblBacteria" id="AAM01819">
        <property type="protein sequence ID" value="AAM01819"/>
        <property type="gene ID" value="MK0604"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0604"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.640"/>
    <dbReference type="HOGENOM" id="CLU_1118194_0_0_2"/>
    <dbReference type="OMA" id="ELNCHCR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dus" evidence="1">
        <location>
            <begin position="63"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="244" mass="26560" checksum="CB9D6BBB537931E4" modified="2002-06-01" version="1">MSGHTGPELAPELERHGVGMVILGGYPGDDATYRASKLVARRRDEFAVPPDRLPEELSRVDVEIPVAVNVRFADPREAGELSGELADLVDVVEINAHCRQPEIVRAGAGEALLEDRTRLIEIVEAVAEHDVYVSVKLRGPHPAFEDALEALRDSSVDVLHVDAMKPGEPTYELEYVRAAARVGVPVIGNNSVRTPEDVDRMFEAGASAVSVGRPILEGDWDVLERLVRYTVLRKSSEGAGLART</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV9</accession>
    <name>Q8TXV9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="2">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gltB_1</name>
        <name evidence="4" type="ordered locus">MK0550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0550"/>
    <dbReference type="EnsemblBacteria" id="AAM01765">
        <property type="protein sequence ID" value="AAM01765"/>
        <property type="gene ID" value="MK0550"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0550"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.585"/>
    <dbReference type="HOGENOM" id="CLU_023342_1_1_2"/>
    <dbReference type="OMA" id="CAWGIAT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="2" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glu_synthase" evidence="3">
        <location>
            <begin position="52"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="429" mass="45720" checksum="93EFB17C07F1D9E2" modified="2002-06-01" version="1">MNAKGPLTRSTPSPVCGGTSRLWWREYVKEIQRKAETGEYAVRGFGTSRKVPHFDDLVILPAQVSRPPIDKYREPCNTKTVLGDRFAEKPLKLDTPVLVGAMSFGALSKEAKVAIARGTAMVGTATNTGEGGMLPEEREEAKWLIAQYASGRFGVSAEYLNAADAIEIKIGQGAKPGMGGHLMGEKVTKEIAEIRGIPKGSDALSPARHMDIVGPEDLKMKIEQLREITDWKIPIIVKYSPGRVKEDVKIAAKAGADIIAIDGMQGGTGASPEIATENAGIPTIAALVQAVEALNEIGMRDEVDIIISGGIRDGADVAKALALGADAVYVCTSVLIAMGCTACAQCHSGRCPVGICTQDPELRKKLDVDEAAERVANYLKVVTEECKMLAQLAGKTDVHNLEKEDLRALSEDVARITGVKMAGSDVELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW2</accession>
    <name>Q8TXW2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01762">
        <property type="protein sequence ID" value="AAM01762"/>
        <property type="gene ID" value="MK0547"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0547"/>
    <dbReference type="HOGENOM" id="CLU_2662349_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="75" mass="7759" checksum="4E3B29C09590B65F" modified="2002-06-01" version="1">MRKTNLAALVSAVALLGALVLAFAGGPRWPVGVLALIVGGLDVMISFRRGGDRVLGLMGFLLLMVGIAVLTGRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX1</accession>
    <name>Q8TXX1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Coenzyme F420-reducing hydrogenase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0538"/>
    <dbReference type="EnsemblBacteria" id="AAM01753">
        <property type="protein sequence ID" value="AAM01753"/>
        <property type="gene ID" value="MK0538"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0538"/>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="INDEFCT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="184"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="3">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="238" mass="25776" checksum="45EBEA5AE4340D6C" modified="2002-06-01" version="1">MKVAVLSLMACGTCARNLEEVCHEEGHEVVWSNLEGDEGEPDADILFVEGAVDFADAETLEKLEEAASAVSTVVSLGSCAATGGFERHVIGMRDPDRYHFTVFPAAKFVETDYAINGCPPTTDVIASFLRALEEENEDELRPYEVLSGDFHGLTPELSAPVTGPTVPVEDVLLTSNKDLCLGCDLDVIEKDLFCVGCGTCAASCPAWAIEMDEKPVIRQERCVRCGTCFVSCPRSFRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX9</accession>
    <name>PYRC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotase</fullName>
            <shortName evidence="1">DHOase</shortName>
            <ecNumber evidence="1">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrC</name>
        <name type="ordered locus">MK0530</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXX9"/>
    <dbReference type="SMR" id="Q8TXX9"/>
    <dbReference type="STRING" id="190192.MK0530"/>
    <dbReference type="EnsemblBacteria" id="AAM01745">
        <property type="protein sequence ID" value="AAM01745"/>
        <property type="gene ID" value="MK0530"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0530"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.564"/>
    <dbReference type="HOGENOM" id="CLU_015572_1_2_2"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000147271" description="Dihydroorotase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <sequence length="426" mass="47525" checksum="9B1AF1432C41557B" modified="2002-06-01" version="1">MLALRVLLKGKILIGGRVREREVLAEDGWIVKIGKRLNEEADLTLELGKRELAVPAPIDLHVHCRDPHPDYPFGFKTETRRFLLGGVATVVDMPNTRPAPTTPETYYEKEELARENAEIEVIVAGGVRDSQCTKELVEAGAYVLGEVFLATSTDAPAVPWSTLPEIFRELSPDGPLTIFHPELDELVAERPARNLYEHLKNRPPEAETTAVGLLAGLKVRYPSRIHLSHVTLPESVKIAKGADITVDVTPHHLFFDVLRVDPEDPVFKVNPPLRGRHHRLGLLRAVRRGDVDVLASDHAPHILEDEFEDVPSGVTGGEIILPAALTLHRRFGLSLRDAVAMITCRPAKLLGRDDLGEVAVGKRARITVVRMREFVVRAEEFGEEDRKFPYDGMRMFGEPVKLIDGTKVYDLKESRREGKPVILEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY0</accession>
    <name>Q8TXY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD family hydrolase, a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0529</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01744">
        <property type="protein sequence ID" value="AAM01744"/>
        <property type="gene ID" value="MK0529"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0529"/>
    <dbReference type="HOGENOM" id="CLU_142246_0_0_2"/>
    <dbReference type="OMA" id="PYIGIGN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="158" mass="17042" checksum="960305F7E38D9E7F" modified="2002-06-01" version="1">MIEVEIPGRGELRLEHLVLDYNGTIASGGKLLESVVEPLQELTEIVHVVVASADTYGTVEDELNDAGLDIEVYRVSAGNEREDKAELIEELGPEVCAAVGNGANDELMLRRAALGICVIGPEGACSRTLLNAHVVVREPREALELLLDPKALRATMRC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY4</accession>
    <accession>Q49598</accession>
    <name>MER_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="2">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mer</name>
        <name type="ordered locus">MK0524</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1995" name="J. Bacteriol." volume="177" first="7238" last="7244">
            <title>Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri.</title>
            <authorList>
                <person name="Noelling J."/>
                <person name="Pihl T.D."/>
                <person name="Reeve J.N."/>
            </authorList>
            <dbReference type="PubMed" id="8522533"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1991" name="Arch. Microbiol." volume="155" first="593" last="600">
            <title>Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase (coenzyme F420-dependent) from the extreme thermophile Methanopyrus kandleri.</title>
            <authorList>
                <person name="Ma K."/>
                <person name="Linder D."/>
                <person name="Stetter K.O."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="1953299"/>
            <dbReference type="DOI" id="10.1007/BF00245355"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-27</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="300" first="935" last="950">
            <title>Structure of coenzyme F(420) dependent methylenetetrahydromethanopterin reductase from two methanogenic archaea.</title>
            <authorList>
                <person name="Shima S."/>
                <person name="Warkentin E."/>
                <person name="Grabarse W."/>
                <person name="Sordel M."/>
                <person name="Wicke M."/>
                <person name="Thauer R.K."/>
                <person name="Ermler U."/>
            </authorList>
            <dbReference type="PubMed" id="10891279"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3909"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Requires the presence of relatively high concentrations of either sulfate or phosphate for maximal activity.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">6 uM for methylene-H(4)MPT</KM>
            <KM evidence="2">4 uM for reduced coenzyme F420</KM>
            <Vmax evidence="2">435.0 umol/min/mg enzyme (at 65 degrees Celsius and pH 6.8)</Vmax>
            <text evidence="2">kcat is 275 sec(-1).</text>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer composed of two loosely associated dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mer family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="2"/>
    <dbReference type="EMBL" id="U31567">
        <property type="protein sequence ID" value="AAA92085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="1EZW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.65 A"/>
        <property type="chains" value="A=1-349"/>
    </dbReference>
    <dbReference type="PDBsum" id="1EZW"/>
    <dbReference type="AlphaFoldDB" id="Q8TXY4"/>
    <dbReference type="SMR" id="Q8TXY4"/>
    <dbReference type="STRING" id="190192.MK0524"/>
    <dbReference type="EnsemblBacteria" id="AAM01739">
        <property type="protein sequence ID" value="AAM01739"/>
        <property type="gene ID" value="MK0524"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0524"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.558"/>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_2"/>
    <dbReference type="OMA" id="VCWAAEA"/>
    <dbReference type="BRENDA" id="1.5.98.2">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q8TXY4"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00697"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q8TXY4"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03555">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000084808" description="5,10-methylenetetrahydromethanopterin reductase">
        <location>
            <begin position="2"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="4" ref="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA92085." evidence="4" ref="1">
        <original>N</original>
        <variation>K</variation>
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA92085." evidence="4" ref="1">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA92085." evidence="4" ref="1">
        <original>I</original>
        <variation>L</variation>
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="5"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
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            <begin position="16"/>
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    <feature type="strand" evidence="5">
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    </feature>
    <feature type="strand" evidence="5">
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    </feature>
    <feature type="strand" evidence="5">
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        </location>
    </feature>
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        </location>
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    <feature type="strand" evidence="5">
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        </location>
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        </location>
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    <feature type="strand" evidence="5">
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    <feature type="strand" evidence="5">
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        </location>
    </feature>
    <feature type="helix" evidence="5">
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            <begin position="221"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
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            <begin position="224"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="232"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
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    <feature type="helix" evidence="5">
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        </location>
    </feature>
    <feature type="turn" evidence="5">
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    <feature type="helix" evidence="5">
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    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="1953299"/>
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    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="8522533"/>
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    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1EZW"/>
        </source>
    </evidence>
    <sequence length="349" mass="37501" checksum="12AF28EDF65F26F0" modified="2007-01-23" version="3">MAEVSFGIELLPDDKPTKIAHLIKVAEDNGFEYAWICDHYNNYSYMGVLTLAAVITSKIKLGPGITNPYTRHPLITASNIATLDWISGGRAIIGMGPGDKATFDKMGLPFPCKIPIWNPEAEDEVGPATAIREVKEVIYQYLEGGPVEYEGKYVKTGTADVNARSIQGSDIPFYMGAQGPIMLKTAGEIADGVLVNASNPKDFEVAVPKIEEGAKEAGRSLDEIDVAAYTCFSIDKDEDKAIEATKIVVAFIVMGSPDVVLERHGIDTEKAEQIAEAIGKGDFGTAIGLVDEDMIEAFSIAGDPDTVVDKIEELLKAGVTQVVVGSPIGPDKEKAIELVGQEVIPHFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ1</accession>
    <name>Q8TXZ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NMD protein affecting ribosome stability and mRNA decay</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nmd3</name>
        <name evidence="2" type="ordered locus">MK0517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0517"/>
    <dbReference type="EnsemblBacteria" id="AAM01732">
        <property type="protein sequence ID" value="AAM01732"/>
        <property type="gene ID" value="MK0517"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0517"/>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="OMA" id="CHMCGSY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="PTHR12746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMD3" evidence="1">
        <location>
            <begin position="3"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="354" mass="40139" checksum="B29D7553649C9C40" modified="2002-06-01" version="1">MICHRCGREVDTTIDGLCPECYLEEHPMIEVPEGLEVRVCAQCLARHTGLRWEDPPEGVGSIEELLVEYVLRELEENLRTLPDVYVRIKPLEVKGEPGGPGARVLVELLAEGEWEVGGEVLTRKYHLKVPVVFALCDRCMKFRSGYYEAILQVRSLRGKLTEREREEVENFVTEAAASLLERDPMAYISDVEYPEEGIDFYIGSLNAARKLARRLVDAYGGTVGESHKLVGFDRERSKRKYKAAISVRIPHFRKGDILLVDGQPYLVTGLGERCSLRHLISREVVKRKWEELKGVEVLKPEPAVVIEDTPPRVVLERTGETVECYESDVDLHVGQRVYVILLKGVAVVVPEEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ5</accession>
    <name>Q8TXZ5_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="7">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">top6A</name>
        <name evidence="9" type="ordered locus">MK0512</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0512"/>
    <dbReference type="EnsemblBacteria" id="AAM01727">
        <property type="protein sequence ID" value="AAM01727"/>
        <property type="gene ID" value="MK0512"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0512"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.544"/>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="OMA" id="CTRSLMR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="PTHR10848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="SSF56726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TP6A_N" evidence="8">
        <location>
            <begin position="62"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="39671" checksum="9D43B0133414AE5B" modified="2002-06-01" version="1">MSEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKGSKKLAQMLSVAAFAKELVEKGRSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLKREEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNIPPNVDAVEILDCGAERVIAVETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWHIYMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPRYYDPFWKKELDLLEKLGKKAEQQAFAKYSLDYVVKEYLPAKLDELE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY09</accession>
    <name>PURL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="synonym">purL_2</name>
        <name type="ordered locus">MK0497</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY09"/>
    <dbReference type="SMR" id="Q8TY09"/>
    <dbReference type="STRING" id="190192.MK0497"/>
    <dbReference type="PRIDE" id="Q8TY09"/>
    <dbReference type="EnsemblBacteria" id="AAM01712">
        <property type="protein sequence ID" value="AAM01712"/>
        <property type="gene ID" value="MK0497"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0497"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.527"/>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100514" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="298"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="517"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <sequence length="724" mass="78777" checksum="A291CB5C84E7D331" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY11</accession>
    <name>TFE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor E</fullName>
            <shortName evidence="1">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfe</name>
        <name type="synonym">tfa1</name>
        <name type="ordered locus">MK0495</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFE family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01710" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01710.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY11"/>
    <dbReference type="SMR" id="Q8TY11"/>
    <dbReference type="STRING" id="190192.MK0495"/>
    <dbReference type="PRIDE" id="Q8TY11"/>
    <dbReference type="EnsemblBacteria" id="AAM01710">
        <property type="protein sequence ID" value="AAM01710"/>
        <property type="gene ID" value="MK0495"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0495"/>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="OMA" id="FENDFLC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation from RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000326605" description="Transcription factor E">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="1">
        <location>
            <begin position="9"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="188" mass="22605" checksum="ADA129A9E2A768C5" modified="2008-04-08" version="2">MYAELGEEDLEVLRDVTLSLLDSEKGVDPEVAKRTVDVILKREAIDEEIAEELGVDPREVRKVLYKLHERGVVTFRKERREEYRYPVYSWRLNLREVLRRCLEERRRELEEVERALSNDMSHPMFHCGNDDCPRMSFEEAMEHEFRCPKCGEVLEEVDLTEERRELERLAEELKVEIRRLEELRERLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY22</accession>
    <name>Q8TY22_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0484"/>
    <dbReference type="EnsemblBacteria" id="AAM01699">
        <property type="protein sequence ID" value="AAM01699"/>
        <property type="gene ID" value="MK0484"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0484"/>
    <dbReference type="HOGENOM" id="CLU_143947_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014515">
        <property type="entry name" value="UCP921964"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09893">
        <property type="entry name" value="DUF2120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021964">
        <property type="entry name" value="UCP921964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="180" mass="19982" checksum="8B70673A76C19FFC" modified="2002-06-01" version="1">MIVPGTPIHIHRLAKKIMMELDAFEGSRPLLDDVDVLIVRGMSRGEDWDLDNLQGYLESVLERCDVEVVDPESPEGKELIEELGKFVVERIQAQYADEEPVSVRPVENPMEAVDAFTQGEIFLDPLGFERLKRDLEALGCVAAYTFGRTPDDLGVFLAAWADRSGIGPKSVELLVANLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY39</accession>
    <name>Q8TY39_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01682">
        <property type="protein sequence ID" value="AAM01682"/>
        <property type="gene ID" value="MK0467"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0467"/>
    <dbReference type="HOGENOM" id="CLU_2597746_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="8415" checksum="0C3E5A8DAF3CF525" modified="2002-06-01" version="1">MRSHLCVLSLSLVGMAAILLLEHSIYAKAGGVLLLASSVIFLVCARLEHVLHRTEQLAVVAAILLILGVSIHALRVGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY44</accession>
    <name>Q8TY44_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaQ</name>
        <name evidence="2" type="ordered locus">MK0462</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0462"/>
    <dbReference type="EnsemblBacteria" id="AAM01677">
        <property type="protein sequence ID" value="AAM01677"/>
        <property type="gene ID" value="MK0462"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0462"/>
    <dbReference type="HOGENOM" id="CLU_077329_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="145"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="21201" checksum="51389280E8C6FC6A" modified="2002-06-01" version="1">MLQDFLNHILSREGSKRLLDAHTSREIMQRPPRFRDFPDVDLDRCILCGACADACPVEGRDGCPPAMEMSEEGPVLHKERCIRCGLCVEVCPTGAIEMGTLHEEVEERVQPPKPARIVVDSDLCVGCGKCESACPSDAITVEETAEVDEERCVLCEVCLEVCPVAGAIKLVPTDTDELVKRWKEYLEASLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY58</accession>
    <name>Q8TY58_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetolactate synthase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ilvB_1</name>
        <name evidence="2" type="ordered locus">MK0448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01663">
        <property type="protein sequence ID" value="AAM01663"/>
        <property type="gene ID" value="MK0448"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0448"/>
    <dbReference type="HOGENOM" id="CLU_1615317_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="23"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="164" mass="17472" checksum="0B60D6358ED509F2" modified="2002-06-01" version="1">MRPEEALLALRKVLKEEDRVIVDLGDHTIAAAKVGLDPDGGTGALGGSMSVALGHCLGTDGGRVYCIVGDGGFFMHLHTLATVAQNRDRFENLTVVVVTDAAWGMTGGQENPAVHTSPADIARSMGFDPAEIAESVEEALEVLKRCRKEGPSLVEMRCRPITFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY62</accession>
    <name>Q8TY62_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein of the translin family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0443"/>
    <dbReference type="EnsemblBacteria" id="AAM01658">
        <property type="protein sequence ID" value="AAM01658"/>
        <property type="gene ID" value="MK0443"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0443"/>
    <dbReference type="HOGENOM" id="CLU_099315_0_0_2"/>
    <dbReference type="OMA" id="EKTHGDI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033956">
        <property type="entry name" value="Translin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="PTHR10741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741:SF2">
        <property type="entry name" value="PTHR10741:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="SSF74784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="217" mass="24964" checksum="461122229BB56FD5" modified="2002-06-01" version="1">MEDLHHRGTSVSEIENLRETIENIREELDALDEARDEAQVILRDLHKLSSEAVYEVHRGNLEEARDKLDEAAELVSELHDLLGDFPELLRTGFAENHLQEYAEAEILYSIVKDRRAPSPEEINVSPRAYLLGLLDAVGELRRIVVDALREGDLDRAEEFLNVMEEIYSLTMTFDYPRAVVPNLKRKQDVARSLLERTRSEVTIAGKTEELKKTLERE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB5</accession>
    <name>RNP3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 3</fullName>
            <shortName evidence="1">RNase P component 3</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp3</name>
        <name type="ordered locus">MK0387</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYB5"/>
    <dbReference type="SMR" id="Q8TYB5"/>
    <dbReference type="STRING" id="190192.MK0387"/>
    <dbReference type="EnsemblBacteria" id="AAM01602">
        <property type="protein sequence ID" value="AAM01602"/>
        <property type="gene ID" value="MK0387"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0387"/>
    <dbReference type="HOGENOM" id="CLU_074509_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140040" description="Ribonuclease P protein component 3">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <sequence length="244" mass="28043" checksum="10F8CBBD23D0AD2F" modified="2002-06-01" version="1">MRVSENFALRVHVDEVDPLRMALAAERLDYEIAVLCLELEAERLNIDDLRWLIEEIRDIREHVESVLVLPGCKLEAESAGALRRAIRRTRPLVYLLAVGGGDPKINRAAVSDTRVDLLSHPERGNPHAGLGKYEIELAREKWTYVEIDLSRLFRREGERLAWQVSRIRDLLRLRRRKRFPTTVALGARDPLELIRPKQVEDLLKLMGFEDSEVKEMCVEAPREILRWNAACKHVFTVPGVVSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB8</accession>
    <name>Q8TYB8_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0384</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0384"/>
    <dbReference type="EnsemblBacteria" id="AAM01599">
        <property type="protein sequence ID" value="AAM01599"/>
        <property type="gene ID" value="MK0384"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0384"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.410"/>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="OMA" id="AVNPQMD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="PTHR10927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="SSF109728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="SSF89895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBDS" evidence="3">
        <location>
            <begin position="9"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SBDS_C" evidence="4">
        <location>
            <begin position="101"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="240" mass="27371" checksum="1D19741560B28780" modified="2002-06-01" version="1">MARVSLEDAVVARLEKGGERFEVLVDPEGARKFREGEDVDVEEILAVEQVFRDARKGERASEQAMEELFGTSDPIKVAEIVIKEGEIQLTAEQRRRMQEEVKRKIIHIIARRAVDPRTGAPHPPERIERAMEEAGVHIDPMKSAEEQVKDVIKQLRPVLPMKFEEVKVAIRIPAKYTGQAMGVVREFGDIEREEWQYDGAWVAVVRLPAGLQDEFFEKLNEITKGDFESKILERESVEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC8</accession>
    <name>Q8TYC8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01589">
        <property type="protein sequence ID" value="AAM01589"/>
        <property type="gene ID" value="MK0374"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0374"/>
    <dbReference type="HOGENOM" id="CLU_1773195_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="15736" checksum="7EAF65328146D5C0" modified="2002-06-01" version="1">MRILALILLLSLPLTPALAEPTPSAGYSSPSEQPAQEEVKTEEQMVETSNVPPSEAVRPSTPEREKEVTTTTSAAGSSETPEITKSEKTNEKKENRVVNLREPNPVEKKNNANVTMLMGILAASALGAYSIAKRKRREALVKVPAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD5</accession>
    <name>HIS1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="1">ATP-PRT</shortName>
            <shortName evidence="1">ATP-PRTase</shortName>
            <ecNumber evidence="1">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisG</name>
        <name type="ordered locus">MK0365</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYD5"/>
    <dbReference type="SMR" id="Q8TYD5"/>
    <dbReference type="STRING" id="190192.MK0365"/>
    <dbReference type="EnsemblBacteria" id="AAM01580">
        <property type="protein sequence ID" value="AAM01580"/>
        <property type="gene ID" value="MK0365"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0365"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.387"/>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000151883" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <sequence length="283" mass="30875" checksum="1DF51CDE1A4BAA97" modified="2002-06-01" version="1">MITVAVPNKGRLHEPALKLLERAGIGVEEPLGRRLKARTTDPDIEVMFVRAADIPRLVEEGVAQLGITGYDLIVEAGAEVKELLDLRFGRARLVLAVPEESDVKSPEDLDGGTVATEFPNIARQYFEDVGVDVEIIQVSGATEIMPRIGVADAIVDLCSTGTTLKVNRLRVVDELLETSARLIANPDATDGEVIRRVYLSLKGVLNADGKCLVMMNVPRERLEEFHELLPGVTGPTVSEIYGDEDMVEVYAVVNEEDVSEVVLRAKELGAEGIIVLPIERMIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE3</accession>
    <name>Q8TYE3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peroxiredoxin, predicted regulator of disulfide bond formation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0357"/>
    <dbReference type="EnsemblBacteria" id="AAM01572">
        <property type="protein sequence ID" value="AAM01572"/>
        <property type="gene ID" value="MK0357"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0357"/>
    <dbReference type="HOGENOM" id="CLU_088479_0_0_2"/>
    <dbReference type="OMA" id="KPTIFLM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="218" mass="23496" checksum="D1DD585931B1A69A" modified="2002-06-01" version="1">MKPDRRLDVRGAACPGPSVMVAEELKEMEPGQVLEVIVDSEGIANDIRELVQDGGHEVLKVEETDGDIRMLIRVGGVETDSTATDGTVRTCAGSLPSTDTDSVMIAQSTGVANPERAYATFLMSEAALAMDLDVVIFGFMDGVTVLVEREVERIRHPEFPPLIDKAREVLRNVEACACELSIQARGITSDDLLDGVKVVGASKFLKLITDPGVDVVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF2</accession>
    <name>Q8TYF2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0348</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01563">
        <property type="protein sequence ID" value="AAM01563"/>
        <property type="gene ID" value="MK0348"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0348"/>
    <dbReference type="HOGENOM" id="CLU_1412372_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="192" mass="20991" checksum="75DA387A77E5D4F2" modified="2002-06-01" version="1">MNARIYAAASVLCLALLAASVQWDVLADLPEAWRELNAATELAHKALEEKKKGDELMEEVSATVNAARSAGPLALSVNAPRILEDLHGAYVHYARAVDAIDAAVDHLDRTLDMIGATADPEIKESLKLMHEGVSHYRMALKEFKLGVQAADEGDVGEAFRHVNAAYRMMREGMKLFSRGKAELESKTLLFMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF5</accession>
    <name>Q8TYF5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01560">
        <property type="protein sequence ID" value="AAM01560"/>
        <property type="gene ID" value="MK0345"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0345"/>
    <dbReference type="HOGENOM" id="CLU_1521879_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="176" mass="19285" checksum="81E181C7BF3BBD8B" modified="2002-06-01" version="1">MRLLKAGDRIQGPGWEGKVTAVLDVDGCRVVITSSNPENVPKRVRGGGTVCELAFHDPVGGFAAYWVEPDHSEATDVGSPEEGTCAVHIGNRWILAEVLEVGRRELSIRIPNVAIPGPGDAGAPVAQNERVVGMLYMYFLEDDCVGRAVRMDVIEERTERALKRLRGRQRARSIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYI4</accession>
    <name>Q8TYI4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-22 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01530">
        <property type="protein sequence ID" value="AAM01530"/>
        <property type="gene ID" value="MK0313"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0313"/>
    <dbReference type="HOGENOM" id="CLU_1544211_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="18234" checksum="F310F752BDD9B373" modified="2002-06-01" version="1">MLLPALLTLLLLPGPVAGAPVDHAVDLPSEGSVSGGDLDRECVEAVLDSCSSVRWCVVRAPGVEEGAVFERCGERWVEVGSGDCPGRCGVLLRGLDAARVSWASGTLRVSGPVGASAWLLVPDPRGLRVGGTFLQGGAYPEGAGVFRDRRVSVRRGRSRCPGRCRLGWLASRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL2</accession>
    <name>Q8TYL2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase of the beta-lactamase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0284"/>
    <dbReference type="EnsemblBacteria" id="AAM01501">
        <property type="protein sequence ID" value="AAM01501"/>
        <property type="gene ID" value="MK0284"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0284"/>
    <dbReference type="HOGENOM" id="CLU_074581_0_0_2"/>
    <dbReference type="OMA" id="HCHPDHY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="19"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="256" mass="28112" checksum="5A7727AE7AE78D05" modified="2002-06-01" version="1">MFFAGTGGGRFAMITQKRRTGGFRLEFPDFHLHVDPGPGCLLSTRLSRRPTTAVDAVFVSHSHPDHYAEAEVLVEAMTRGGTKRRGTFVGSVSAVEGYSDDSGDYGPVLSAYHRSLPEEVVALEPGDEIDLPAGTLEAVPTTHGDPTGIGFRLETEDDSIYYTGDTELREDFFEVIEDVTVAIVNVVRPGSDRIRGHLCTYDVIELVSEAPELRKVFITHFGMKMLRVGPGREARRIEHETGVETVAARDGRAYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP8</accession>
    <name>Q8TYP8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase fused to uncharacterized archaeal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cysH</name>
        <name evidence="2" type="ordered locus">MK0245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0245"/>
    <dbReference type="EnsemblBacteria" id="AAM01462">
        <property type="protein sequence ID" value="AAM01462"/>
        <property type="gene ID" value="MK0245"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0245"/>
    <dbReference type="HOGENOM" id="CLU_632571_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="1">
        <location>
            <begin position="238"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="420" mass="46847" checksum="4793B42D9798DD31" modified="2002-06-01" version="1">MHLHVLVRARKDASAVESALERYYPDWEWKVETLGGERDPSWIVGLARERFEGSDALWKVLLLGRRSYPGRGRVVWMEGWSAVNVGKAEVRNARLTEIVDGIELGRAAPRLSVAFRRVPELRRGRLLTENQDADTMLRWGKFTETIAELAGEELPPGAYFSLRKPGNPPEEVLYDASGKPILRIITPDEGEPSVSVLGDGEPFDSRAFVRENRETLENLFEDAVDFLGGVAEEFDVLFVPVSGGKDSTCCLAIAVETTDRLGIDVEAVYVDTGYDLGRDVVEDVGAALGVDIKHVDVSDAFERGLRERKPTHENRWCTGVKLSGIKKVLKDLEGDVLLVVGDRDAESRRRRLRPPVHRNRLLDVPEVNPVKGWGGAEVLGTLFRLGLPVSELYEIGFYRLGCSVCPSLTAWERALMELSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYS2</accession>
    <name>Q8TYS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative snRNP Sm-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">lsm1</name>
        <name evidence="4" type="ordered locus">MK0220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0220"/>
    <dbReference type="EnsemblBacteria" id="AAM01437">
        <property type="protein sequence ID" value="AAM01437"/>
        <property type="gene ID" value="MK0220"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0220"/>
    <dbReference type="HOGENOM" id="CLU_076902_11_1_2"/>
    <dbReference type="OMA" id="GYDVHMN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01731">
        <property type="entry name" value="archaeal_Sm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="LSM_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022901">
        <property type="entry name" value="snRNP_Sm-like_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="PTHR10553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0543">Viral nucleoprotein</keyword>
    <keyword evidence="4" id="KW-0946">Virion</keyword>
    <feature type="domain" description="Sm" evidence="3">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="73" mass="8146" checksum="3C90B90CDCA824AB" modified="2002-06-01" version="1">MRPHDCLRDFLDDIVIVELKTGKTLRGRLVSFDGHLNLVLDDCVEIDEDSEVRLGRVLIRGDSVTLISPAEVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-19" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT7</accession>
    <name>PYRG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">MK0205</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYT7"/>
    <dbReference type="SMR" id="Q8TYT7"/>
    <dbReference type="STRING" id="190192.MK0205"/>
    <dbReference type="EnsemblBacteria" id="AAM01422">
        <property type="protein sequence ID" value="AAM01422"/>
        <property type="gene ID" value="MK0205"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0205"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.206"/>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
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            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
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</entry>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
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                <person name="Aravind L."/>
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        </location>
    </feature>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="272" mass="30293" checksum="19009AF754050BEC" modified="2002-06-01" version="1">MLDVTRLPGDRLLISSDIHVGDEYQGHDRETWEAALDLARDFDAFLIDGDLADPRASDPELRELLRDLRELSSEVPTYFVPGNHDTVDLVKSLRDAGVHVLSRRYDNRRGRGCPPGHSGPSLGGPHLLRFGDAWMLVLHGHEPCSELGLNPQKPVNPVARESPMPKRDQILDNYTCREYEMPDRLEEIARSTHADVVITGHTHCRYLGSLEGKLVVNVGTTSCPAICATCRDPLNVGNVCILKASGGTLRAKLFNLRKARVIGRERVRIGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ25</accession>
    <name>Q8TZ25_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0115</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="4 5" key="2">
        <citation type="journal article" date="2018" name="Proc. Natl. Acad. Sci. U.S.A." volume="115" first="3030" last="3035">
            <title>Enzymatic reconstitution of ribosomal peptide backbone thioamidation.</title>
            <authorList>
                <person name="Mahanta N."/>
                <person name="Liu A."/>
                <person name="Dong S."/>
                <person name="Nair S.K."/>
                <person name="Mitchell D.A."/>
            </authorList>
            <dbReference type="PubMed" id="29507203"/>
            <dbReference type="DOI" id="10.1073/pnas.1722324115"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="6CI7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-377"/>
    </dbReference>
    <dbReference type="PDB" id="6CIB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-377"/>
    </dbReference>
    <dbReference type="PDBsum" id="6CI7"/>
    <dbReference type="PDBsum" id="6CIB"/>
    <dbReference type="SMR" id="Q8TZ25"/>
    <dbReference type="STRING" id="190192.MK0115"/>
    <dbReference type="EnsemblBacteria" id="AAM01332">
        <property type="protein sequence ID" value="AAM01332"/>
        <property type="gene ID" value="MK0115"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0115"/>
    <dbReference type="HOGENOM" id="CLU_732837_0_0_2"/>
    <dbReference type="OMA" id="CEVVERH"/>
    <dbReference type="BRENDA" id="6.2.2.1">
        <property type="organism ID" value="3274"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017667">
        <property type="entry name" value="Methan_mark_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03266">
        <property type="entry name" value="methan_mark_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00702">
        <property type="entry name" value="TIGR00702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="63"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6CI7"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="6CIB"/>
        </source>
    </evidence>
    <sequence length="377" mass="42622" checksum="D006104BEB173B02" modified="2002-06-01" version="1">MTDIVYDVEGFRAFLPKETLRWIRHRELERKVGVVEKFSDRVGPIPVEIRRRRSQYGEFYHAGKGTTRIQARVSAAMECVERAAAEPREEIIERGPEGDKWTPAWYRTEPREWVEGVDLTTREPVYVPANEVFHPWLGDALPSHTNGLSAGRLREEAVIQGLLEVVERDSWSIVEYFRIHPPELEVHGELEELRRSLEREVGRVELRLLPSRVEGVYVVGAVTEAERVEEMVMGFGASPDPEMAVLRALLEVAQGLSMARRGIESPVRKGLGEFSAPGKLTPERLKRLNRHWFEPEGTVEIDDLDRVITTGSLEKLTEELVERVAEAGLGKVIEVDLTLENLDVPVVRVRVTGASEYVIDEARVGNMPEKPPGVPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ51</accession>
    <name>Q8TZ51_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rqcH</name>
        <name evidence="3" type="ordered locus">MK0088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0088"/>
    <dbReference type="EnsemblBacteria" id="AAM01305">
        <property type="protein sequence ID" value="AAM01305"/>
        <property type="gene ID" value="MK0088"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0088"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.88"/>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="OMA" id="MVYGKRN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT-R_1" evidence="2">
        <location>
            <begin position="474"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="289"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="414"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="671" mass="77555" checksum="E2815D70CE79012E" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ60</accession>
    <name>Q8TZ60_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrA_1</name>
        <name evidence="8" type="ordered locus">MK0079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0079"/>
    <dbReference type="EnsemblBacteria" id="AAM01296">
        <property type="protein sequence ID" value="AAM01296"/>
        <property type="gene ID" value="MK0079"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0079"/>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="189"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="270" mass="30050" checksum="7B5B4DED9D8CFC67" modified="2002-06-01" version="1">MISYLGPPGTFTEEAAERFSEEELGGNGKLQSARTITAVFSRVERGEADYGVVPWENSLEGSVGETLDNFLHRSVRVFGELVLPIVHALMSGSEPDDREPVVYSHPQAYEQARETLRELLGNHEFVPTASTAEAAKLASKEGAYALGPPRLAERFGLDIMEEIRLENNETRFAIISRRDRAPTKEDKTSVVFSVTDRPGALREILGIFADRGINLTKIESRPAKRGLGDYVFFLDFEGHRMLYPGSEALAELRERTPFSKVLGSYPKVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ71</accession>
    <name>Q8TZ71_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01285">
        <property type="protein sequence ID" value="AAM01285"/>
        <property type="gene ID" value="MK0068"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0068"/>
    <dbReference type="HOGENOM" id="CLU_1340738_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="23009" checksum="65DBDAB9D7B5D11E" modified="2002-06-01" version="1">MRWKVTAALVATLVISPALVHGSFDHRFQKYLRMERLQSAVSDIDGATKDVATVLASSPYLAYKAALYDNTLRVGNDPGRDRLELLSAIRSAEHKLELARLKIRKARAKGVTGLDRAERNVSRALSALHRAEDRVEKRAPGVTDDLREIDALTPVLRTLTERKRWLVQEITSKLPKFKYYELSENLLYLPPFNILFPLVGAFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA3</accession>
    <name>Q8TZA3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C4-type Zn-finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0032"/>
    <dbReference type="EnsemblBacteria" id="AAM01249">
        <property type="protein sequence ID" value="AAM01249"/>
        <property type="gene ID" value="MK0032"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0032"/>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="OMA" id="HSAIIGD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="Znf_ZPR1-type_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="PTHR10876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="zf-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zpr1" evidence="5">
        <location>
            <begin position="10"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="201" mass="23040" checksum="1E6C815F924A2CE5" modified="2002-06-01" version="1">MKYKMELSDLECPVCGEKALMVHGRVDEIPHFGRVLEQFIHCKACGYRHSDVMCLEDREPAEYRYRVNSPEDLRVRVVRSPSGFVEIPELGIEVKPGPAAQGFVSNIEGLLRRIRERVETAAKWADKEESKKRAEEILRRMDAAVSGEDEITIVLKDPYGHSAIVPEEDDKLEVRELDEEEAEELRSRIFRPLVPSGEYSN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-01-25" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZB4</accession>
    <name>MTIP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable S-methyl-5'-thioinosine phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioinosine phosphorylase</fullName>
            <shortName evidence="1">MTI phosphorylase</shortName>
            <shortName evidence="1">MTIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0021</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + S-methyl-5'-thioinosine = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:30643"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.44" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZB4"/>
    <dbReference type="SMR" id="Q8TZB4"/>
    <dbReference type="STRING" id="190192.MK0021"/>
    <dbReference type="EnsemblBacteria" id="AAM01238">
        <property type="protein sequence ID" value="AAM01238"/>
        <property type="gene ID" value="MK0021"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0021"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.21"/>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="OMA" id="MTQCPEA"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415142" description="Probable S-methyl-5'-thioinosine phosphorylase">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <sequence length="256" mass="28790" checksum="C3DD32D388C8D4ED" modified="2002-06-01" version="1">MTEVGVIGGTGFQPGLPERRRTVFTPYGTVRVDITRVGDHRVYFINRHGKGHDLPPHRINYRAIVWAMRELGVKRILATNSVGVINSDEYEPGDIVLPVDFLDFTKRRPTTFYDEKVVHVDVTEPYCPELREALLKAADDLGYTVKEGAVYVATEGPRFETPAEIRAFRKLGGDIVGMTGFPEVVLARELEICYASVCLCTNYAAGIDDRRRTIDEVFELVEELRPKAVELIERCIEYIPPKRSCPCSQALEGAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVE3</accession>
    <name>F1SVE3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type molibdate transport systems, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02736">
        <property type="protein sequence ID" value="AAM02736"/>
        <property type="gene ID" value="MK1523"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1523"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="OMA" id="GVIHEMG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="7">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="228" mass="25119" checksum="94AE09A3E835DB83" modified="2011-05-03" version="1">MLRVEGVKFSYGDREVLRGVDLEVEPGEVRVLFGPNGSGKSTLLKIVAGILRPDEGRVIIGEEDVTDLPPEERHVGYVPQQPALFPHMTVEDNITYSLQNGRGDPDRLDELVELLGLKEHLDRKPDELSGGYQSRVSLARALFSDPKVMLLDEPLSDVDLAVKAGLVPRFREVLKETGIPALYVTHDPWEAERIGDSFTVLIGGKAVDVNSVDEALEELKRGVESVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVE8</accession>
    <name>F1SVE8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl coenzyme M reductase, subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mcrD</name>
        <name evidence="2" type="ordered locus">MK0652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0652"/>
    <dbReference type="EnsemblBacteria" id="AAM01867">
        <property type="protein sequence ID" value="AAM01867"/>
        <property type="gene ID" value="MK0652"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0652"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.691"/>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="OMA" id="ELEVFPH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="173" mass="19662" checksum="9F46AA1D0B298E0E" modified="2011-05-03" version="1">MLERKIPEGAKPEVQIFPQRLLSADTTEKLLNELLERVEGIGRIVIHGPGLPKAVPFGPARGKPVKHPERRPIEVHGEKIPMKVQTGQIIVEIEDEDRLDDIVEEIEKICREILPCDFEVQVGRFTRHKPTVTDYLKFGEEGVKKLDKRLLGLVEPRARLADRVSVLKKKGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVM5</accession>
    <name>F1SVM5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0014"/>
    <dbReference type="EnsemblBacteria" id="AAM01231">
        <property type="protein sequence ID" value="AAM01231"/>
        <property type="gene ID" value="MK0014"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0014"/>
    <dbReference type="HOGENOM" id="CLU_081185_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009573">
        <property type="entry name" value="HcgC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06690">
        <property type="entry name" value="HcgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019265">
        <property type="entry name" value="DUF1188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="252" mass="28339" checksum="797666180E9C8073" modified="2011-05-03" version="1">MKEPEPGITDLTETVRSRPFWDVVKEIGLAKAEEIAEVVPEGSRILVLGAYLTGIFVAELLSEEHEITVLDPEPALRKILPPGVRFRASRVPPPGRYDVVIDLTGLGGTNPRVLRRLNPEVLVVENPAGNMNDPRIEEYNDTEERLEAGEESYELRLFDAPFEAKTSGTFTLSVRTVREAANRLERHYGVLYAVPGVVNLERWTFRLRRPEEGVERAREKPAVTVSQLTGSSDPDEVIGEVLDEILFEIRER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>P94954</accession>
    <name>MCH_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber>3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mch</name>
        <name type="ordered locus">MK0625</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1998" name="Extremophiles" volume="2" first="15" last="22">
            <title>Overproduction and one-step purification of the N5,N10-methenyltetrahydromethanopterin cyclohydrolase (Mch) from the hyperthermophilic Methanopyrus kandleri.</title>
            <authorList>
                <person name="Vaupel M."/>
                <person name="Vorholt J.A."/>
                <person name="Thauer R.K."/>
            </authorList>
            <dbReference type="PubMed" id="9676239"/>
            <dbReference type="DOI" id="10.1007/s007920050038"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Structure" volume="7" first="1257" last="1268">
            <title>The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri.</title>
            <authorList>
                <person name="Grabarse W."/>
                <person name="Vaupel M."/>
                <person name="Vorholt J.A."/>
                <person name="Shima S."/>
                <person name="Thauer R.K."/>
                <person name="Wittershagen A."/>
                <person name="Bourenkov G."/>
                <person name="Bartunik H.D."/>
                <person name="Ermler U."/>
            </authorList>
            <dbReference type="PubMed" id="10545331"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)80059-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the reversible interconversion of 5-formyl-H(4)MPT to methenyl-H(4)MPT(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text>Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MCH family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01840" version="1"/>
        </conflict>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAA70072" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.4.27"/>
    <dbReference type="EMBL" id="Y08849">
        <property type="protein sequence ID" value="CAA70072.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01840.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="T45099">
        <property type="entry name" value="T45099"/>
    </dbReference>
    <dbReference type="PDB" id="1QLM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-316"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QLM"/>
    <dbReference type="AlphaFoldDB" id="P94954"/>
    <dbReference type="SMR" id="P94954"/>
    <dbReference type="STRING" id="190192.MK0625"/>
    <dbReference type="EnsemblBacteria" id="AAM01840">
        <property type="protein sequence ID" value="AAM01840"/>
        <property type="gene ID" value="MK0625"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0625"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.663"/>
    <dbReference type="HOGENOM" id="CLU_876031_0_0_2"/>
    <dbReference type="OMA" id="DKGMFAP"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00694"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="P94954"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00545">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02289">
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    </dbReference>
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        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000140882" description="Methenyltetrahydromethanopterin cyclohydrolase">
        <location>
            <begin position="2"/>
            <end position="316"/>
        </location>
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        <location>
            <begin position="4"/>
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    <feature type="helix" evidence="2">
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        <location>
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    <feature type="strand" evidence="2">
        <location>
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    <feature type="strand" evidence="2">
        <location>
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    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="307"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1QLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="34044" checksum="D6D4097DE6BE0500" modified="2007-01-23" version="4">MVSVNENALPLVERMIERAELLNVEVQELENGTTVIDCGVEAAGGFEAGLLFSEVCMGGLATVELTEFEHDGLCLPAVQVTTDHPAVSTLAAQKAGWQVQVGDYFAMGSGPARALALKPKETYEEIDYEDDADVAILCLESSELPDEDVAEHVADECGVDPENLYLLVAPTASIVGSVQVSARVVETGLYKLLEVLEYDVTRVKYATGTAPIAPVADDDGEAMGRTNDCILYGGTVYLYVEGDDELPEVVEELPSEASEDYGKPFMKIFEEADYDFYKIDPGVFAPARVVVNDLSTGKTYTAGEINVDVLKESFGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY5</accession>
    <name>CCA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CCA-adding enzyme</fullName>
            <ecNumber evidence="1">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cca</name>
        <name type="ordered locus">MK1366</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TGY5"/>
    <dbReference type="SMR" id="Q8TGY5"/>
    <dbReference type="STRING" id="190192.MK1366"/>
    <dbReference type="PRIDE" id="Q8TGY5"/>
    <dbReference type="EnsemblBacteria" id="AAM02579">
        <property type="protein sequence ID" value="AAM02579"/>
        <property type="gene ID" value="MK1366"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1366"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1518"/>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="OMA" id="DVDLVPC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="PTHR39643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="SSF55003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0692">RNA repair</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000139070" description="CCA-adding enzyme">
        <location>
            <begin position="1"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <sequence length="485" mass="55774" checksum="0D5F13590F3E14BB" modified="2002-06-01" version="1">MSLEEVLDEIRREVTPDPEERELVEGFARRILSEVRDRLKERDPDAEVELIGSVARDTWLPGASDVDVFCVFPKDRDLDEIVEVTLEVGREAIEALGGEAREEYAHHPYIGGEVEHRGRTFEVDVVPCYDTEPGEVITPVDRTPHHNRYVEEHLEDTVEARLLKAFVKAIDAYGAEVRVKGFSGYLCELLAIHYGSFEEVLREAVRTWRPGFVIDLEGFVGEVYEDYDEVRETFEDQDPALIVLDPVDPERNVAAALSRRQLTRFILAARAFLGDPSPEFFRGRKPEPVSGEKVRDWFERNPTHVVAIEVRLPDEVEDIYWPQLEKTARSLSRVLENEGFEVRRWHVMRDSEEEHGYVLLEFEHGKLPELEWRVGPSGWVREDRVRGFVRAHGRFWVEEDGKLATRAERKFVRPEDLLGRLEGADRQTLLSHGFGKDLARSSEGEVRLLSAEELAELADRDPELGKALAEFMRGDPLSELVRDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY8</accession>
    <name>Q8TGY8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lhr-like Superfamily II helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lhr</name>
        <name evidence="3" type="ordered locus">MK1035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1035"/>
    <dbReference type="EnsemblBacteria" id="AAM02248">
        <property type="protein sequence ID" value="AAM02248"/>
        <property type="gene ID" value="MK1035"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1035"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1087"/>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="OMA" id="YARTQSF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like_ATP-dep_RNA_helic_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="31"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="228"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="910" mass="102476" checksum="94A057AF68F2CB7F" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ0</accession>
    <name>Q8TGZ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNAse P subunit P29</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pop4_1</name>
        <name evidence="2" type="ordered locus">MK0846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02059">
        <property type="protein sequence ID" value="AAM02059"/>
        <property type="gene ID" value="MK0846"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0846"/>
    <dbReference type="HOGENOM" id="CLU_2191039_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
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        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="SSF101744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="108" mass="12107" checksum="81AD3CDB4CA648E6" modified="2002-06-01" version="1">MRPDRTSETHTSVNTDSSYRRGFDTLTPSPLPPPPDPEELVGKKFRVVRANSRTYLGISGRVVGFTKNCLVYDDGGGDQKILPLRHIVMEVEGLRVDGRTLLRLLQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TH02</accession>
    <name>Q8TH02_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0043"/>
    <dbReference type="EnsemblBacteria" id="AAM01260">
        <property type="protein sequence ID" value="AAM01260"/>
        <property type="gene ID" value="MK0043"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0043"/>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="5">
        <location>
            <begin position="170"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="4">
        <location>
            <begin position="261"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="444" mass="50440" checksum="DB7DDEABD729B28D" modified="2002-06-01" version="1">MKVSEIVSMLARILAEHYRSGVSLKESFYRALDNPFSIPSHRARAIHRRLMELGKRLGLCEEILDDVIQSGSFEDLGPELKGVLVTAADEMLFEGTSPALVTDAATRVAKELVSDRVADFVHAVCFDLEKYDVDRLKRRGYDDLCLRHYFPPDFVDYVRRVLPEDEIEDFLRACNKPAVKYVRVNTLRADVDEVRERLAEEGVLTEPDEHIPDLLRVVEEEIPIVRTEAWKEGLVFTQDKASAAVAHVLDPQPGEFVVDLCAAPGGKTLHALCLMEGEGEILAVDKSDWRLDAMREKLAWQRVPDGVVELRCADAREIPEELDEEADRAIVDPPCSGMGSVQKRPETRWNVTKKRVRRYAKLQSELLEAAIKTVRPGGIVVYSTCTLTINENENVIRRVARRYDVTIEKVNLQFGRRGLVPGTRRFYPHTDRCQGFFIAKLRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV4</accession>
    <name>Q8TUV4_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="6">ATase</shortName>
            <ecNumber evidence="6">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="6">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">purF</name>
        <name evidence="12" type="ordered locus">MK1649</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6 9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6 10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1649"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AAM02862">
        <property type="protein sequence ID" value="AAM02862"/>
        <property type="gene ID" value="MK1649"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1649"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1812"/>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="6 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6 8">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 10">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AAM02862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="451" mass="49993" checksum="EAE2DD7E54F9D4E6" modified="2002-06-01" version="1">MCGVSGCYLLKENEAGVYNYLILHANQHRGQESAGICVYDGLRLVGKKGMGLVTEVFDRPDLRKLSGPVGIGHVRYSTTGASELVNAQPFKVGYSKGELALAHNGDIVNSEELRRELVSDGHAFVSETDSEVIARLLAVALTETDDMFEAFEDVMERLVGSYSLTVITSHGDLIAVRDPWGFRPLCLGWDERGFFVSSETVGLDVLGVEERRELERGEVVWIREGDVESKVVRRERKAVCMFEFVYFARPDSIIEGRCVYECRKCMGKRLAEEAPVECDLVVPVPDSGRTAALGYAESLGVPMEEGLIKNRYVGRTFIMPEQEERVRSIRVKLNPIREVIKGCSLAVVDDSIVRGNTSRQIVEMLRDAGAREVHMRIASPPVVSPCYYGIDMATKEELIAADLDVPEICEKISADSLAYLSLEGLVESIRLKKRELCVGCLTGEYPTPVPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW7</accession>
    <name>Q8TUW7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02849">
        <property type="protein sequence ID" value="AAM02849"/>
        <property type="gene ID" value="MK1636"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1636"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="204" mass="24072" checksum="0BDCB4A89CA4922B" modified="2002-06-01" version="1">MVRSHAPIPYNPVERIRALRVLREVHRRRKKPSLEVTYRTVNGSTCGPYYVARWRRDPRHKHGRTLYLGKPENESVSFVEWLVSLDRREVLELARHLMRNLRSVLKTLLTEVSDLPYKKARWVLARGLALAFDARPSESPRIRDLLEELPDRLESFAVRTLGGWPAHYSSYLRKVIHHRGKSLDEKHEVPDVGLEFQRWKLQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW9</accession>
    <name>Q8TUW9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1634"/>
    <dbReference type="EnsemblBacteria" id="AAM02847">
        <property type="protein sequence ID" value="AAM02847"/>
        <property type="gene ID" value="MK1634"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1634"/>
    <dbReference type="HOGENOM" id="CLU_030571_5_1_2"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="16"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="211" mass="22553" checksum="94BA1812CD31BD2F" modified="2002-06-01" version="1">MRVFRVGGKGITPSPDCNCYLLAVGDEGILIDLGASGEVIDRLPGNVDVRYALLTHSHFDHAAAGPDALEAGLEVGVHRAEAEVLREGDDRLSAAYLFGRPMPAYEPSFTFRDGETFDVGGEEVEVLYTPGHSPGSCCFLLGDLAFTGDTVFGFGPGRWDLPGGDRKKLCESLERLLSTGVRSIFPGHGYEVIGEAVPAIEAALREAEKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUX7</accession>
    <name>Q8TUX7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin fused to cHTH-type DNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1625"/>
    <dbReference type="EnsemblBacteria" id="AAM02838">
        <property type="protein sequence ID" value="AAM02838"/>
        <property type="gene ID" value="MK1625"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1625"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1788"/>
    <dbReference type="HOGENOM" id="CLU_1639975_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="119"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="161" mass="17720" checksum="6A0E28EB32030523" modified="2002-06-01" version="1">MIQGWSALRRGRLNLEAVIRALAAERMASELPLVEVAARLDVDPSTVSHYLSGDYPSEETRDKVRRVVEETPPFGLWPQLREALGVDVAAEALKWVMGPRTEAEPDVDDERCLACGRCSEICPSPDGECLGCGECVRACPSGARSLSVRYRGLVYRVFSPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUY5</accession>
    <name>IF6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 6</fullName>
            <shortName evidence="1">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif6</name>
        <name type="ordered locus">MK1616</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUY5"/>
    <dbReference type="SMR" id="Q8TUY5"/>
    <dbReference type="STRING" id="190192.MK1616"/>
    <dbReference type="EnsemblBacteria" id="AAM02829">
        <property type="protein sequence ID" value="AAM02829"/>
        <property type="gene ID" value="MK1616"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1616"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1779"/>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="OMA" id="GEDTTGP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="PTHR10784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153747" description="Translation initiation factor 6">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <sequence length="221" mass="22980" checksum="816FF01591BF35B1" modified="2002-06-01" version="1">MTVVKASVHGDPNIGAWIAASEEYAVVAPKVPDDIVERVKEALDVEVVRTTVAGSNLVGALLAVNSNGALFPRHAREHEIRVVRELGVEVDVLPSKMNAVGNLVLTNDHGALVHPDLDDHALEVIESVLGGRVVRGELGGVKTVGSAGVANSKGAVVHPGATEEEMERVSEVLGVDVEVGTVNRGSPYVGVGIVVNSKGAVVGEDTTGPELARLEDALYLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV07</accession>
    <name>IXTPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1594</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV07"/>
    <dbReference type="SMR" id="Q8TV07"/>
    <dbReference type="STRING" id="190192.MK1594"/>
    <dbReference type="PRIDE" id="Q8TV07"/>
    <dbReference type="EnsemblBacteria" id="AAM02807">
        <property type="protein sequence ID" value="AAM02807"/>
        <property type="gene ID" value="MK1594"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1594"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1755"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_2"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000178275" description="dITP/XTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <sequence length="188" mass="20899" checksum="A575787DE25B1EF0" modified="2002-06-01" version="1">MKVLFATGNIGKYHEAKQILARYGIEVERVDLDYPELQSDSLEEIAAYGARYCAESLGQPVIVEDSGLFIEALNGFPGPYSAYVFDTIGNEGILKLLEGEENRKAEFISVVGYCEPGGRPVTFTGEIRGRIAEEPRGEEGFGYDPIFIPEGEDSTFAELGVEEKCKISHRTKALERFAEWYKNNVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV09</accession>
    <name>Q8TV09_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">recJ_1</name>
        <name evidence="2" type="ordered locus">MK1592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1592"/>
    <dbReference type="EnsemblBacteria" id="AAM02805">
        <property type="protein sequence ID" value="AAM02805"/>
        <property type="gene ID" value="MK1592"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1592"/>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="OMA" id="ACGAYIP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA1" evidence="1">
        <location>
            <begin position="314"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="442" mass="48398" checksum="7C31E7BDA7181CFB" modified="2002-06-01" version="1">MGVKRGGTLSDDGFEGTLEEAVEFLEDLEGTAVRLYVHNDADGLTAGALMAFTLRCLEVPTRLRVLNTETELLKEDLDGPTVVVDMGSGILDKLRNDHPVLVIDHHEISSKPSDNVLLVNPREHGVDGGTEASASTVAYLLCRRVVKVEETCLPKAALVGAYGDNQAGKRGVRGLNRVPEEDGEKHEIIEIRDPAYWVFGRASMTVGEIVERVSGASVREALEARFGDLDVAPVDLSREDEAEIVREHRELLSGEKRKELESKTGRIHVFDEERPLKALRDPLETATLLNACGRYGEGWAGVLIAMGSWDPLQLAKKLRSKHKAIIREALSRLSRGEGITDRESMVVIDGRELGIPHTVVGIVAQFVCEERERVTVGVAEMEEGLVKVSIRCPENSDVDAAEVIKEAADRVDGEGGGHERAAGAEVPEDRLEEFLLELEKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV40</accession>
    <name>Q8TV40_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Predicted permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1560</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02773">
        <property type="protein sequence ID" value="AAM02773"/>
        <property type="gene ID" value="MK1560"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1560"/>
    <dbReference type="HOGENOM" id="CLU_056175_5_2_2"/>
    <dbReference type="OMA" id="TTFFWTL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="298" mass="31315" checksum="3FC1611EC2484CEB" modified="2002-06-01" version="1">MGGLEAFLEILGLVFVGYTLRKVGVFSKDTPSHLNAYVVYLAMPALVFTAMLRAPIQGLLQHLKLVVISMLLSVMCALIGFAVARKMARDRRDAMAVALASGLGNTGFLGYPVCLSTFGEPGLEAAVFYDFGTTLSVIAAYLMLSSRRENPLKMSLRFPPAHAAVAGMCWAALGLHLPDTIRSTLQLLGRSVVPVIMVSLGASLRWDLPKSVISVIPTVWVVKLCASPVVAATIAPSGLDGKVAVLEAAMPPALMNLVLAELLGLRPHVTAALVFSTTVISLATVPTVIYLVSGGGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV87</accession>
    <name>Q8TV87_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1506"/>
    <dbReference type="EnsemblBacteria" id="AAM02719">
        <property type="protein sequence ID" value="AAM02719"/>
        <property type="gene ID" value="MK1506"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1506"/>
    <dbReference type="HOGENOM" id="CLU_1017848_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="273" mass="30470" checksum="CEBBF91A42F95E16" modified="2002-06-01" version="1">MKAAVINRSKGTVLAHSAEIAESFLARLRGLMFRRRLEEGEGLILKPPYRGRRRCAIHTFFMRFPIDVVFLADGEVVDVVERLKPWRVYVPEEPPEEIVELPAGIVSATDTEVGDSVEVVAGDLESELAVRILNRKLVTRKLAQVIMRAVAKSGLQGEVFYNKDTGCIHISGEDKYIVADTIKETFGDSVEIVEGRAKILSDDHSLVVIVKDAHSVLEKIGISRPGGELMYRKEPKPVRWGKQREKGADDGSEQAEEGDRNEGEGRTRHRGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVA0</accession>
    <name>Q8TVA0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein implicated in tolerance to divalent cations</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cutA</name>
        <name evidence="2" type="ordered locus">MK1492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1492"/>
    <dbReference type="EnsemblBacteria" id="AAM02705">
        <property type="protein sequence ID" value="AAM02705"/>
        <property type="gene ID" value="MK1492"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1492"/>
    <dbReference type="HOGENOM" id="CLU_098807_3_1_2"/>
    <dbReference type="OMA" id="VYTTFPD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="PTHR23419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="102" mass="11762" checksum="AA9AA5F0912ACF32" modified="2002-06-01" version="1">MFVVVYSTAEDEEEAKRIARKLVEEDLAACVNLWPIRSVYEWGGELCEDEEYALLVKTTAERAEEVVERIVELHSYETPAVLVLPVLGGFEGFLEWIREQTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVB7</accession>
    <name>RL18E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18e</name>
        <name type="synonym">rpl18A</name>
        <name type="ordered locus">MK1475</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVB7"/>
    <dbReference type="SMR" id="Q8TVB7"/>
    <dbReference type="STRING" id="190192.MK1475"/>
    <dbReference type="EnsemblBacteria" id="AAM02688">
        <property type="protein sequence ID" value="AAM02688"/>
        <property type="gene ID" value="MK1475"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1475"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1631"/>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="OMA" id="WRLERPR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_L18/L18-A/B/e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_L18e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_L18e_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="PTHR10934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132791" description="50S ribosomal protein L18e">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <sequence length="127" mass="14322" checksum="C6375E3BCA7C0C1F" modified="2002-06-01" version="1">MTWAPTGPTNVELRKLIRDLKKAACEYNAPVWRDVAERLSRPRRQRAEVNVGKLDGLARRGVIQEEETVLVPGKVLGDGVITQPLRVAAWRFSRTARMKIEAAGGECLTIRELLEENPEGSYIRIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI4</accession>
    <name>Q8TVI4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02618">
        <property type="protein sequence ID" value="AAM02618"/>
        <property type="gene ID" value="MK1405"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1405"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1560"/>
    <dbReference type="HOGENOM" id="CLU_234960_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1912"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1942" mass="217170" checksum="33EAB83EFE8A3487" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVN5</accession>
    <name>Q8TVN5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-45 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02566">
        <property type="protein sequence ID" value="AAM02566"/>
        <property type="gene ID" value="MK1353"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1353"/>
    <dbReference type="HOGENOM" id="CLU_1003297_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="277" mass="30678" checksum="872BBDA9BB9E5F3A" modified="2002-06-01" version="1">MHLASRISTDRRARAKAEGLTAFGSALQTLSVGIAAALGTRLGDGEVEVTNRAVESLGIRPPFDEEKYLRGLTSVRDDPNRAYETMYRALWRWPRKVFRELHRTLEEDQAAFAGIVDYLWEEIIRQARESPALDEFVREVGKGWKVTREAAEATGLAGSIAGTVGDLLRRSLSRLHGGSEGDRDRSERRDEHRSWRLGDAISQYSGSSEGTSPLSKRTGDAPRGRGEVARDPGFDGVDGAGRSGEVPRTRPREPEGTARGGRGTGRGRRGPWRWLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVP0</accession>
    <name>Q8TVP0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02561">
        <property type="protein sequence ID" value="AAM02561"/>
        <property type="gene ID" value="MK1348"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1348"/>
    <dbReference type="HOGENOM" id="CLU_1122628_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="247" mass="27677" checksum="E84979C3CF9F0EF7" modified="2002-06-01" version="1">MREITDSPLAFIPALLGPVAGLYVTINGLSVEIRCRHPAGWMYNDAAVILPVTAFRDLLFPLVSVVIFKTAIERLRPLGPFGLFVAPVIAAAWEWAAERAFGAVLDRWYSRTTLAPLVEEPFKRLVAIELGVPGVVSGLAYGLLEGLWKSRTLWGRKDVVSLLRYIALNYVFVHALLTVLPLPIAMEVHYHLNASWFPEPEKVHLLVIQIFRDLLTGDIRTMLSMSYYTILSLMLESTFGIPVMMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR1</accession>
    <name>Q8TVR1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized domain specific for M.kandleri, MK-12 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02540">
        <property type="protein sequence ID" value="AAM02540"/>
        <property type="gene ID" value="MK1327"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1327"/>
    <dbReference type="HOGENOM" id="CLU_1529239_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="175" mass="18132" checksum="596353344551632B" modified="2002-06-01" version="1">MLCGVGVGLISVVPWLALFAFPYWYLARHALPYVDGPLRFELTRRLGTMGAVTGLWCLLAPLVVGMYAPAVAIPAAAVSIVPAVRAVMACLRAPSEFTIALMNVGTWARRTSAAGACVALVATALAGPVVGGLAELASAVACAYVVSSRVGIASHLAKRVWIPTDRCSGQRPAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR2</accession>
    <name>Q8TVR2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK-12 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1326</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02539">
        <property type="protein sequence ID" value="AAM02539"/>
        <property type="gene ID" value="MK1326"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1326"/>
    <dbReference type="HOGENOM" id="CLU_1217576_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="227" mass="25968" checksum="39AD2B0042A58A14" modified="2002-06-01" version="1">MVEPIRSLPGSRTRVKLTRECIVIRERGETRRIRLEDVVGVKEHPRRASKALDPAEDTRLVLITGDLERLVLLVRDGRLYWGLKALLAARDGETDRLIRYLTELLRIAGHPPLLERRDGRVLLRWPWLELELGSPERAETVYRELLSRWEELLESDEKGHDLDREARPVWVALSVPLAPMVMTVGVHRRLIAQVSPVLIWGAKLAVDVLERVLGKGPIQCSVAWESV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-05-16" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS6</accession>
    <name>CAS1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas1</name>
        <name type="ordered locus">MK1312</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVS6"/>
    <dbReference type="SMR" id="Q8TVS6"/>
    <dbReference type="EnsemblBacteria" id="AAM02525">
        <property type="protein sequence ID" value="AAM02525"/>
        <property type="gene ID" value="MK1312"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1312"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1445"/>
    <dbReference type="HOGENOM" id="CLU_838407_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417100" description="CRISPR-associated endonuclease Cas1">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <sequence length="331" mass="36447" checksum="15458A785E32D3CF" modified="2002-06-01" version="1">MGAPNDTVIFLRRGKIERREDAFRIGKSKYSAVRTTGIIIAGGAQITTQAVRLALRNEVPIVYLGGNRILGVTVPFSERYATLRLKQYEIASQPSARLAFARPLIASSILARAAVLEFLANETGITGLEDAADEVRSEAERALNAGSTDALRGYEGRAACRYFRALAEVLPDWAFSGRRTRRPPRDPFNAAISFGYAGVLLPVLLSRTVAAGLEPFLGFLHGPRGRRPGLILDLMEEWRALAVDVPVLRRFLDGSLSREMFRWKGDAVLLRDLDAVSAPVLTVLSRVRGGLLEAVDRRIREVRDGVSRQSPPEPLEFDPEDVGVVWDALEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV2</accession>
    <name>Q8TVV2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri, MK-30 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02499">
        <property type="protein sequence ID" value="AAM02499"/>
        <property type="gene ID" value="MK1286"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1286"/>
    <dbReference type="HOGENOM" id="CLU_870477_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="35772" checksum="74B74C1AA7FC518F" modified="2002-06-01" version="1">MCSARSSRRSSPCPGPRSPTAGPRSPGVHLGEWTELTVTGSTVQAHLIVGIRGYGLEGSFEVPIKVLVPSDAELVTAKWKPKGGGEVEVEPVSVRTVTWPGDTDQIGYLLRYRSEDPLGALFPDGVIRWRELTFVLPVEAKGSESSRCLFDVFKGEYLGTLDVTVTARLHRGKGEEGEAWFFAWPIPESPNPAYDVKVHGLYVDFVTVYDPETGRWRWFIPVIQPKDRSSTYVYVRVDVAGPVFTFGVETDHVSVETVYPWDERASQFLSLTQYLPVLVVMKKELQRGEANGETASPGRGGGGAGSTRTGRTVPLIVPIVPVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVV5</accession>
    <name>Q8TVV5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fusion of at least two uncharacterized domain specific for M.kandleri, MK-2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02496">
        <property type="protein sequence ID" value="AAM02496"/>
        <property type="gene ID" value="MK1283"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1283"/>
    <dbReference type="HOGENOM" id="CLU_512540_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="531" mass="59053" checksum="F4CE1FBAC69ABD27" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW6</accession>
    <name>Q8TVW6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02485">
        <property type="protein sequence ID" value="AAM02485"/>
        <property type="gene ID" value="MK1272"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1272"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1388"/>
    <dbReference type="HOGENOM" id="CLU_494023_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="551" mass="59925" checksum="E9499B4100348630" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX1</accession>
    <name>Q8TVX1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-37 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02480">
        <property type="protein sequence ID" value="AAM02480"/>
        <property type="gene ID" value="MK1267"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1267"/>
    <dbReference type="HOGENOM" id="CLU_631081_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="441" mass="50006" checksum="7416CBCFE41F9BF1" modified="2002-06-01" version="1">MPECVPLSPTDRFYDKVKSYVRHRMSDLGLRIGDAALYDTKYGPLSPLPIFMDGWWRGSWIRERRLWDGKLDKPWLLTVSVLAELFDSNPEEIAGIPSIEIEPGASKLGYMLRKMIKTGEESLKEKREYGHLSDYILWALEVIEVITRRIRDLVLDKPSLETALNFWVIGAVLSSRNVSKVTVISDSVDLEIPKSSVEIPLPYGINIQGSSSITSTLYVIAHAMVHRGIDEDQLLEHFNIQTVCALWIEENMHKKMIKKGLEELKHGFEKLLKKSKWELELEDLGIDDPEAFFHEVSKKLAEIVTNVSTAFETIQDKPEEIARNLLSVDEEIRLGDEVIPPEEYDPKWRQSQRVKQHVKGTLESIDTEKLAEDALKTVGRLVGGSKGEEMAETAAKLATEVVKRGLDMVSESSGNRKERSVLGELIEAVVKEVMDGARKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY2</accession>
    <name>Q8TVY2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized domain specific for M.kandleri, MK-34 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02469">
        <property type="protein sequence ID" value="AAM02469"/>
        <property type="gene ID" value="MK1256"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1256"/>
    <dbReference type="HOGENOM" id="CLU_862242_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="322" mass="36455" checksum="216A56330FAEEE46" modified="2002-06-01" version="1">MDKSSLYGLALDVTTLAVLRLVKRYPASGKGFPEGKNSVELVLGEAKDLFRRFELEVLAGERELLEACFEVLRLPGVPVKVGGACGKAGLETLRKVFTDPDASVLFITSYGLDEFEEVMEELGVDPEEIRAECRRLLVQEVEYEGRVHPSEEVIESWVEAGFEVRVVGREKFEKKGVHFEVCYTDNVMAITSANMTTAGLERLRECSELVVPLVMDVKAPVALLEYEPLGFAAVVGANVHAGVLHEFLTRSDRVYDRRSSYRNFYEEYGGYLPECEGLRHLYRELRAGLEWYLREGCPEMLWWVEPEPQTMPDVTPNLNLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW39</accession>
    <name>Q8TW39_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">MK1198</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02411">
        <property type="protein sequence ID" value="AAM02411"/>
        <property type="gene ID" value="MK1198"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1198"/>
    <dbReference type="HOGENOM" id="CLU_079909_0_0_2"/>
    <dbReference type="OMA" id="GVSQPHW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM02411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="202" mass="21296" checksum="1336C0CC0D8DF0C1" modified="2002-06-01" version="1">MGLEALDPYGVLKGVIELFVVVDPIGNVPALLAVTSELRPRDRVRVVHRAVAFAAVLILGFAVAGKATLDYLGISVEALMIAGGILLLRAAFGMVEGDPTGFRIEPGSHIDVAYVPLGTPLLAGPGAIVTVIVMLHRHGRLETILACLIVMLLTLVTFRAAERLARFLGRSGIRVLTRVMGVLLAAIGVQMVLDGVSAFVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW49</accession>
    <name>Q8TW49_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted extracellular polysaccharide hydrolase of the endo alpha-1,4 polygalactosaminidase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1187"/>
    <dbReference type="EnsemblBacteria" id="AAM02400">
        <property type="protein sequence ID" value="AAM02400"/>
        <property type="gene ID" value="MK1187"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1187"/>
    <dbReference type="HOGENOM" id="CLU_1324028_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016062">
        <property type="entry name" value="TM1410-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35882:SF1">
        <property type="entry name" value="PTHR35882:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01545">
        <property type="entry name" value="THEMAYE10DUF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_114" evidence="1">
        <location>
            <begin position="67"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="325" mass="36982" checksum="37515554FA2B1623" modified="2002-06-01" version="1">MESYRTLLAVLLMGLLFPSSWVVGKVRVESTGMPFATYYQNVKMSEVLQKRPRVLVLDPWCGPNGRPWTKEELRKIKDAGVKPIAYLPVCVVAEYHPNLYREARRRNLLGADDPEWPGDYAVKFWEPAWRDVLRSELARLKDLGFEGVFLDVVDAHSRDWYVRWYDRVNPHGDLRRDELNAVKWIAQTAHGLGLRVVVNAGAWAFEGDGMARLQSRLGFAVTVESVLSDGSRRLYSRSELESNLRTLSRFQGPVYVIEYGLDPDDPSVRRAAEKLFDKTRATCVYITSVKHDGIGVALVPLRRWLWDVTLGPLLGEPPSWIGGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW77</accession>
    <name>Q8TW77_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-26 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02372">
        <property type="protein sequence ID" value="AAM02372"/>
        <property type="gene ID" value="MK1159"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1159"/>
    <dbReference type="HOGENOM" id="CLU_1003296_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="277" mass="30907" checksum="3A47B316384E39B4" modified="2002-06-01" version="1">MVCGDPRLREIVLEFRDSVRVGSSRVGGVLRKLYPILSLYVILSLVFEGRGVVRVLWDRASAPLAMIVIAGACIAPILAAYLAFHGYLTLEARRRLRAPVVTRPHNPTLLLLPFLLALLRPDYYVHHWPETRVALAYLYAGCALSPTLALAVMWVLRTRVVWCWKRDLDEEVLERFVEGLPDGRYVPGSLIPYASNVGTLAVAIAVDAPSLTPYLITAGTLSVLALTTYISTFTEGDLLDILGKLRSGPWRDDSYVIGFTILILERSFWLIIEHSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW87</accession>
    <name>Q8TW87_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri with repeats, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02362">
        <property type="protein sequence ID" value="AAM02362"/>
        <property type="gene ID" value="MK1149"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1149"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1226"/>
    <dbReference type="HOGENOM" id="CLU_236924_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1784"/>
            <end position="1817"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1849" mass="207434" checksum="9D5458373E381CC9" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW94</accession>
    <name>Q8TW94_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-22 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1142</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02355">
        <property type="protein sequence ID" value="AAM02355"/>
        <property type="gene ID" value="MK1142"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1142"/>
    <dbReference type="HOGENOM" id="CLU_1567157_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="170" mass="19521" checksum="759032008AEEDCC2" modified="2002-06-01" version="1">MRTFQFHPVTTAVLRTPELDEGALFRLRDDRWVEVASGDLPGTFERFFRRLGEARSEWREGVLRVEGPPGAEVVLLVPDPEGFPVTEDSTFLEDGPLPEGAHVLLGERVRAFRTHVPWRVRLPFEVARESLVSVRWVEGSTYYEHSDWAAHDWDRAAVSHPARPRPELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA9</accession>
    <name>Q8TWA9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1127</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02340">
        <property type="protein sequence ID" value="AAM02340"/>
        <property type="gene ID" value="MK1127"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1127"/>
    <dbReference type="HOGENOM" id="CLU_2366211_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="95" mass="10333" checksum="091B3934819A97B5" modified="2002-06-01" version="1">MFIGVLLAPTVNLLLLISLVKLLHVKCSLQGAELIARIAPMMTLGVVPAVTVEARSLLTRIPPTATVERILTVLNVPAVFVYAFSFFILVHCYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWB5</accession>
    <name>Q8TWB5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted ATPase involved in replication control, Cdc46/Mcm family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mcm2_2</name>
        <name evidence="2" type="ordered locus">MK1120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="211" last="222">
            <title>Insights into the architecture of the replicative helicase from the structure of an archaeal MCM homolog.</title>
            <authorList>
                <person name="Bae B."/>
                <person name="Chen Y.H."/>
                <person name="Costa A."/>
                <person name="Onesti S."/>
                <person name="Brunzelle J.S."/>
                <person name="Lin Y."/>
                <person name="Cann I.K."/>
                <person name="Nair S.K."/>
            </authorList>
            <dbReference type="PubMed" id="19217392"/>
            <dbReference type="DOI" id="10.1016/j.str.2008.11.010"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3F8T">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-506"/>
    </dbReference>
    <dbReference type="PDBsum" id="3F8T"/>
    <dbReference type="SMR" id="Q8TWB5"/>
    <dbReference type="DIP" id="DIP-48326N"/>
    <dbReference type="STRING" id="190192.MK1120"/>
    <dbReference type="EnsemblBacteria" id="AAM02333">
        <property type="protein sequence ID" value="AAM02333"/>
        <property type="gene ID" value="MK1120"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1120"/>
    <dbReference type="HOGENOM" id="CLU_538219_0_0_2"/>
    <dbReference type="EvolutionaryTrace" id="Q8TWB5"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="1">
        <location>
            <begin position="217"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3F8T"/>
        </source>
    </evidence>
    <sequence length="506" mass="56695" checksum="60432D9E24BEC2C2" modified="2002-06-01" version="1">MFRLGTTPRATTYDPDARIGEVASRFGLPTRVLIEIVRTESFQRSLRRVTSGKPVVLDLRELDSDLASWIATHARLVEPALRELVRTVAPDVEPRVRFRGLPHRFRRVERIRPMDGALISIEGVVREVRGAERLEHAIVDTGSELVAVRLHGHRLGPGLRVEILGIVRSATLDALEVHKKDPIPEVHPDPAELEEFRELADKDPLTTFARAIAPLPGAEEVGKMLALQLFSCVGKNSERLHVLLAGYPVVCSEILHHVLDHLAPRGVYVDLRRTELTDLTAVLKEDRGWALRAGAAVLADGGILAVDHLEGAPEPHRWALMEAMDKGTVTVDGIALNARCAVLAAINPGEQWPSDPPIARIDLDQDFLSHFDLIAFLGVDPRPGEPEEQDTEVPSYTLLRRYLLYAIREHPKPELTEEARKRLEHWYETRREEVEERLGMGLPTLPVTRRQLESVERLAKAHARMRLSDDVEPEDVDIAAELVDWYLETAMQIPGGDEIRISSLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD5</accession>
    <name>Q8TWD5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted extracellular polysaccharide hydrolase of the endo alpha-1,4 polygalactosaminidase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1100"/>
    <dbReference type="EnsemblBacteria" id="AAM02313">
        <property type="protein sequence ID" value="AAM02313"/>
        <property type="gene ID" value="MK1100"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1100"/>
    <dbReference type="HOGENOM" id="CLU_795997_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyco_hydro_114" evidence="2">
        <location>
            <begin position="43"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="348" mass="40154" checksum="D6560779D46FB29D" modified="2002-06-01" version="1">MLETILLATVAWTVQDHDQPFATYWQDVKFWEILERHPRTLVVDPYCGPDDRPWTKDEIRTLREHGVKPVAYLSLATVGKHQTDLYSLAEEKGLLGPRDPYWEGDRAVRFWERTWLDALRTKFEELRDLGYEGVFIDVVDPWTLDWYVKWFRRETGENLEKLRELTYDALEELIQAALHLGLEVYVNVGGAIFDPKLAELKERYGFKVVVEDVITDDEGNLVPDDVFRDYLRALAHLGSGVYVIEYDLTMTPEVNERLEELFAETKVEAVYVTSLDHDRLGIDIVPIKAPVNPSDTSGSSGEEKASYTRENEEDLERIPVEELVEEGPKKLPVIPIVPPVRRRCLSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD6</accession>
    <name>Q8TWD6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-7 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02312">
        <property type="protein sequence ID" value="AAM02312"/>
        <property type="gene ID" value="MK1099"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1099"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1154"/>
    <dbReference type="HOGENOM" id="CLU_741048_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="377" mass="42110" checksum="8495C7A77415DCED" modified="2002-06-01" version="1">MDWNDPLQPEESALEKLHVAMPKPCETLGDFEIFRRVVLSTLESCEGLAGLLKGEGIEETHPIRFLLEVLDTPSTLAEAAERLIHWTISGVSGLWEGLRGTVDPAEIVLLHFLQLRRFFVRRTPEYLEDPGLESVLEPLSVEVALMACAGGLLEREDLKEAAEYVADGVGVDERVLELLPELGVRYGSLVPRFASPTRVWVIFALLCHTCANRTVPPEELVKPLKELRPGPIDEIEYSVREALHEAVGGLGIDDEEVLRWVGSILVDSVEHALELADTLSRWEELAVKIPGAGAVIRLGEVEIQPGEYDPIWEPGPQEEVHESVVERVRELVEEEANGAVERLYELDMEEAGELVGDDALDDLLDDSIEELRKRRMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWF5</accession>
    <name>Q8TWF5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin-guanine dinucleotide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mobB</name>
        <name evidence="6" type="ordered locus">MK1080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1080"/>
    <dbReference type="EnsemblBacteria" id="AAM02293">
        <property type="protein sequence ID" value="AAM02293"/>
        <property type="gene ID" value="MK1080"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1080"/>
    <dbReference type="HOGENOM" id="CLU_068199_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="122"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="194" mass="20685" checksum="9285705B02E1C7BB" modified="2002-06-01" version="1">MRVLQVVGPKDSGKTSFCEEAVKELRGRGYRVGYVKSVGGHGLDLQDRDTGRVPADVRVGVARKETVLFLDLDIDAVLGLLALLGLDYVLVEGFKSRELGVRVGFGGYTEGPTVPAEEIDTSPADAVERYAVKYTADIDCGRCGPGSCRDFRRAVARGEENPDGCAAPEDVTVLVDGKPLGLNPFVGDLVKSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG6</accession>
    <name>Q8TWG6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-25 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02281">
        <property type="protein sequence ID" value="AAM02281"/>
        <property type="gene ID" value="MK1068"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1068"/>
    <dbReference type="HOGENOM" id="CLU_619145_0_0_2"/>
    <dbReference type="OMA" id="NYYDVRY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="420"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="457"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="481" mass="55679" checksum="6AB44F24D3C5B8FD" modified="2002-06-01" version="1">MKDDKGGRKLFRVRNERHERYLAVAVPLLAAVFLLTTSPVMMNWYDVHYFEEWYRVAMSKGLLHVYPECEKVHYPPMAVVFYITTRDLIHNLGLDSNPWTFQFSLKIFLVAFYLLTCLVIIREFEWPIAQWGVISVPMGMVVWGYQFDTIIAFFLALTAVCVRRGRPIAAGVSLALASSFKYVPGILVIPFTLILKEKYGSRPAKIFFLTSAITVALLWSPFILYDPEAFWKQAFLFHMLRLPQDLTPLNIPLLLTRWHVYPYHFIIGKLSGPLIILGFVTLALWLWKRREILQESIRTSGLKHTIWGSAALFMLWFALTTKVGNPYYIAWIYLWVFPFAVWLLPSWCVGFLNTAPFLLALSRLPAAVCNAPVFVPEDVRWYPALQLLGFSTPLAVEKAHQLYLAAPNFMRLWYYHMHQTELVLVVVYTIVVTKTLLDLLKWIRDPKPADERPHDWAIHPMLLAFTLPAYIFALYIALPIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG9</accession>
    <name>QUEC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name type="ordered locus">MK1065</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG9"/>
    <dbReference type="SMR" id="Q8TWG9"/>
    <dbReference type="STRING" id="190192.MK1065"/>
    <dbReference type="EnsemblBacteria" id="AAM02278">
        <property type="protein sequence ID" value="AAM02278"/>
        <property type="gene ID" value="MK1065"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1065"/>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000246977" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <sequence length="229" mass="25498" checksum="8142F768EBAFAAF5" modified="2002-06-01" version="1">MATTVVLCSGGLDSSVIAKWAVEELGGRVICLFVDYGQRNAPFERRAAERIAEAVGAEFETVGTFWLRRLCPDNPMFAGRLPREAGTEDLSANWLPARNWNLLGVAAALCDHLYLEGEDDEFHIVWGINAEEAERFPDNTKEFADAVAEALKRGLPSRPRLHSPLAELYKPGIVRLGSELGAPMELSVSCYNPIWEDDTPVHCGECEACYHRKRAFERAGIEDPTEYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK2</accession>
    <name>Q8TWK2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Type I signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lepB</name>
        <name evidence="6" type="ordered locus">MK1031</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1031"/>
    <dbReference type="MEROPS" id="S26.011"/>
    <dbReference type="EnsemblBacteria" id="AAM02244">
        <property type="protein sequence ID" value="AAM02244"/>
        <property type="gene ID" value="MK1031"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1031"/>
    <dbReference type="HOGENOM" id="CLU_089996_4_0_2"/>
    <dbReference type="OMA" id="FPKIGLI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015927">
        <property type="entry name" value="Peptidase_S24_S26A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00717">
        <property type="entry name" value="Peptidase_S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S24" evidence="5">
        <location>
            <begin position="47"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="155" mass="17252" checksum="78AC4D5E01AB74B5" modified="2002-06-01" version="1">MGWSALNVKRAWRKVRELRGWKAAVFYVVLGVALGYGLRYGLGFVLGTPDPVVTVISESMYPYYNVGDVLLVVGVPYRDIKVGDVIVYRLPGKPIPVVHRVIAKTPEGVITKGDNNPLPDPWCPIRPKEISGRVVLRIPYVGYPKALLDRYLYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK9</accession>
    <name>Q8TWK9_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1024"/>
    <dbReference type="EnsemblBacteria" id="AAM02237">
        <property type="protein sequence ID" value="AAM02237"/>
        <property type="gene ID" value="MK1024"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1024"/>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="OMA" id="WMGQNTN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="24"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="379" mass="42599" checksum="F82A03F824598F51" modified="2002-06-01" version="1">MGPVSADWIPDSPDELFRKIHSGEVIELGQNTNRLGPPEEVRRAVVRAALEAPYNVYAHPQGDRRLREGLLEYFELDDDFDAVITNGAIEGVHAVIRAFASNGVAVTPDPGYKTIDGMMMAEGVHVQEVDIYSEEFDYQMTVDAVMEEFDGNLRELDLIFVINPSNPLGSSMSERELRGLVELAQDADAFLVHDCTYRDFAPEQPVACEMDPERCVDLYSFSKTYGLAGLRIGAVIGERKLLEQVSKYKVSKLGINLIAQEAAITALKVRDRWIPRLLREVTRNQRLIKRECEGDGVKIPVYPSHANCLVIDFSEYGYIDSKTVVGKLYSEHGIFVRTGYYTSPRRGKGFIRVAFSAPREDIERFCEAFNEVMGELIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL0</accession>
    <name>Q8TWL0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA synthetase (NDP forming)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1023"/>
    <dbReference type="EnsemblBacteria" id="AAM02236">
        <property type="protein sequence ID" value="AAM02236"/>
        <property type="gene ID" value="MK1023"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1023"/>
    <dbReference type="HOGENOM" id="CLU_063044_1_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="22735" checksum="D4686CEF38588DD1" modified="2002-06-01" version="1">MSSEIVLEHDVLTALREGGLKTPRFTVLERPEEASDVPFDPPYTVKLLHSGVAHRARVGAIYTAVPSKERLERRVAELLERWPDAVGVIVQEHLDVVKGIEAFLGIKEDDTFGTVVLLGLGGKLVEELRAYIVRRPPVDGEDVERGLRRVPGGERLLSEIGPNRLAEVVNAAHGVYKEEGWSELDVNPLLLIDGEAIALDGLARKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL1</accession>
    <name>Q8TWL1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1022"/>
    <dbReference type="EnsemblBacteria" id="AAM02235">
        <property type="protein sequence ID" value="AAM02235"/>
        <property type="gene ID" value="MK1022"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1022"/>
    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
    <dbReference type="OMA" id="FMFREES"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="66" mass="7220" checksum="3A3AA19EB2F3FC48" modified="2002-06-01" version="1">MEGKDKPVDVGDVYEVKIEDTGKRGDGVARVNGFVIFVPEAEKGDKVLVKIVSVRDTYAIGRIVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL3</accession>
    <name>Q8TWL3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Mezhevaya K.V."/>
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    <sequence length="340" mass="37225" checksum="0D71268A2A86B6EE" modified="2002-06-01" version="1">MLTVFLLALLISPVGCSGMKLGAVWISRESGYTHHLFLVDNASGALWYLGTDASPGPRPWVLERTLRKPEPLIPGEVHPDALGAVVIPFKPGEWPGVALCSDPTTAATFRRLLDRPELRTILNGPVIVTTEVQGRPCCIVRRTPDGFEFAFEDGTDADYNDAVIEVTLEAVVVSVGELKISIKRESPEEYDVLLLDRSTGSVIRITPDGEASPIIGTLDVHPERLGIALKGEDTVLCSDAETAEVLRKLAEESPELRAFLDGAIIAWTTEVVGRRVDACRGSTKCEHGVWYLRFGFEDGWDGVVDYDDVIVEITGPRGNFNPLSVLPLILMPRGRNPWRS</sequence>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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</entry>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="372" mass="39858" checksum="981353CAAA55FDF6" modified="2002-06-01" version="1">MLLRVPYLPQVHSHRGIPLEAETVAIALTIILAAMALGRAAGMKFRYGILYRATRSVGFMDRWLEHTLLGPVLKVSVRASPILGFAGLAFAIYTLVKGASEGSGGFTVLLPGITLPLISGLASLAVILTVHELGHAVAARLSGIRIKRIGFFLVVVLPGAFVELEEEEFRRAPLRRRIEVLSAGPAFNVLTSFIAMGAVLGLSAIPGYVTSGVMVHGKMFKDVPLHTGEVIREVDGQPVKTIVDLRRALANHKPGDVVQVATDSGTKLVKVHEHRGRPVLGVYVIPNFGGYLVSEVMVALVMFLNMLGMLSLGIGVANLLPIKPLDGGRIVHEVLREVLDPSLASRLSTTVSIVALILLVLNLRAPYHVLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR0</accession>
    <name>Q8TWR0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-8 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02185">
        <property type="protein sequence ID" value="AAM02185"/>
        <property type="gene ID" value="MK0972"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0972"/>
    <dbReference type="HOGENOM" id="CLU_488032_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="558" mass="62492" checksum="F61CB5F34321B66A" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-05-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR1</accession>
    <name>COFE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme F420:L-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.31</ecNumber>
            <ecNumber evidence="1">6.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Coenzyme F420-0:L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Coenzyme F420-1:gamma-L-glutamate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cofE</name>
        <name type="ordered locus">MK0971</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent successive addition of two or more gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2) or polyglutamated F420 derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Monovalent cation. The ion could be potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CofE family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.31" evidence="1"/>
    <dbReference type="EC" id="6.3.2.34" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWR1"/>
    <dbReference type="SMR" id="Q8TWR1"/>
    <dbReference type="STRING" id="190192.MK0971"/>
    <dbReference type="EnsemblBacteria" id="AAM02184">
        <property type="protein sequence ID" value="AAM02184"/>
        <property type="gene ID" value="MK0971"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0971"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1016"/>
    <dbReference type="HOGENOM" id="CLU_051152_1_1_2"/>
    <dbReference type="OMA" id="ANEVCAS"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052619">
        <property type="term" value="F:coenzyme F420-1:gamma-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01258">
        <property type="entry name" value="F420_ligase_CofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023659">
        <property type="entry name" value="F420_ligase_CofE_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145790" description="Coenzyme F420:L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01258"/>
        </source>
    </evidence>
    <sequence length="252" mass="27135" checksum="BDB7BFC01D334E34" modified="2002-06-01" version="1">MGGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEIEPSPFAKTLAERTGKDPRVVEVILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPPGYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRPFREGVVGVAIGASGVPVLADRRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKARAIIRSPSRDIFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR6</accession>
    <name>Q8TWR6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02179">
        <property type="protein sequence ID" value="AAM02179"/>
        <property type="gene ID" value="MK0966"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0966"/>
    <dbReference type="HOGENOM" id="CLU_1232776_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="25905" checksum="2CEDC7D96F91A0D9" modified="2002-06-01" version="1">MNGWLNMSEELSRRYPAVRVPAGELAHSEPTEDGFVTPRGVRFHRALVVGVLSGRYVDEEREFAVITVTDDTGSVDVFAFDECYEPARTLDKWRQIKVIGRPMEPREEGRPVALRAEVIRLTSYSEELFRRLEYELMAGGEVPTVEKPPTLDEAIEMVYKELLEREEETFSREELYEVIRSVVPLADEEFCEKIIKEMEERELLYPMDLPGGKRWSVIETGWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS6</accession>
    <name>Q8TWS6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin, molecular chaperone implicated in de novo protein folding, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0956</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0956"/>
    <dbReference type="EnsemblBacteria" id="AAM02169">
        <property type="protein sequence ID" value="AAM02169"/>
        <property type="gene ID" value="MK0956"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0956"/>
    <dbReference type="HOGENOM" id="CLU_116577_0_0_2"/>
    <dbReference type="OMA" id="DYQFGRG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TGT_C2" evidence="3">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="2">
        <location>
            <begin position="78"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="150" mass="17054" checksum="A0660C566829F63E" modified="2002-06-01" version="1">MSRYVEIVRGILAYQFGREAADAMLDGEVKVRVRRGRPREVFVDGKRLCTVRASDGLVSLAPEGARRLHAATEPPEHRVVCADEWVEAVRRGRDLFCEYALRGWEELRPGDECLVVSEDDELLAVGKMRLSGWEVEYFEHGVAVRVRRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWT5</accession>
    <name>Q8TWT5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein specific for M.kandleri, contains repeats, MK-5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02160">
        <property type="protein sequence ID" value="AAM02160"/>
        <property type="gene ID" value="MK0947"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0947"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.993"/>
    <dbReference type="HOGENOM" id="CLU_543612_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="501" mass="55033" checksum="2055F6105531A3E4" modified="2002-06-01" version="1">MVTIPALIVLFTVLGPVAAGPVNLSQTSGGERVIVAFNPDDPNAWSAVHRHPVGLERGPWAGNFYDARCFPNEVTTHGTKFVFEKSGGLVYGLKVEPGDSITLPEPASVVSVAYAAVCWWENGQPFKRVTLIVEDSHGRQATTSLTLADWCARAFSPHAPDHNFLVCEFDHRLSVEERKIRFFIIPIGITWKWRVAGLKCGVWAFRLSASELGLRDITRIEFRYGDGHLYILAVTITPTGPFDDIVGGPNCSGTTVGPDGMDEVGSCYWEGEWPARFGHTTVTANGVPFLIPPASGPNALLANGRLVELPRPARTVWFLYAATNWWFGDKPYGPVDLVVVDDRGRTVTARVLLVDWCAHDVVPNNIEVLAFNHRAIPGGLQSIHCGVWAFELDAGQLGLPNIKAVYFPSMGRRKVWILGATLDGQPVGLGELRPHRISHPWSGFKRGVVYPGGETLDGTFLLPSECRKVQRTPIEGLADGNAERPLELEDRGRERHLRTRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY8</accession>
    <name>GLMM_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable phosphoglucosamine mutase</fullName>
            <ecNumber evidence="1">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmM</name>
        <name type="ordered locus">MK0893</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWY8"/>
    <dbReference type="SMR" id="Q8TWY8"/>
    <dbReference type="STRING" id="190192.MK0893"/>
    <dbReference type="PRIDE" id="Q8TWY8"/>
    <dbReference type="EnsemblBacteria" id="AAM02106">
        <property type="protein sequence ID" value="AAM02106"/>
        <property type="gene ID" value="MK0893"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0893"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.935"/>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="OMA" id="SIALDEY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000337817" description="Probable phosphoglucosamine mutase">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <sequence length="452" mass="49408" checksum="B9FC12A658F28CB5" modified="2002-06-01" version="1">MGKYFGTSGIRGRVGEFLTPELALRAGRALGEYLGGGTVAVGRDTRVHCDALRAAVISGLTAQGCDVVDIGVVCTPTLGCYVATEGLDAGVMITASHNPPEYNGIKFWDSDGMAFSPEQEREIEQIMDGDLEYPNWDEYGEVVDDETALNVHVERILDEVSVDGDGLRIVVDCANGPSAFVTPVVLREMGCEVISLNAHPDGHFPGREPEPKPENLKDLMRTVRATDADLGIAHDGDADRVVFVTEEGKFAGYDEVLALVCRRILEEKGPGKVAVNVDASMVIDEVVREMGGEVVRTKVGDVHVAAAIREEGCVFGGEPNGTWIHPDVHMCPDGPLSAAWMVSLLIEEGRPLSELLAEIPSYPVVRETVECPDELKPEVMRLVETRLREAYDDIDTVDGVRVELDDGWVLVRPSGTEPLIRITVEAESEERARELRDEFVDIVRRCVEEVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX00</accession>
    <name>Q8TX00_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD(FAD)-dependent dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0881"/>
    <dbReference type="EnsemblBacteria" id="AAM02094">
        <property type="protein sequence ID" value="AAM02094"/>
        <property type="gene ID" value="MK0881"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0881"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.922"/>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="OMA" id="ACGIPYW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="1">
        <location>
            <begin position="329"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="446" mass="47816" checksum="842ADDDFE4B7B22B" modified="2002-06-01" version="1">MRVVVVGGGAAGVVAARTAREHGADVVLISADEHIAYSPCAIPFVISGEIERPEDILMRDPTHYERLGIDVRLGVRVEEVDPEEKVVTTEDGDTVEYDSMVLATGGEPLVPPIEGSELDGVFTVRRFSDIEPLLRAVQESERAVIVGAGPIGVEMAYALHERGLEVTLVEMLDRVLPQFLDDDVAAIVQERMEKEGVRVLLGSPVEAIEGDDRVEAVVVNGEEIEADLVVMAAGVRPVTDLFESIGASVLPFGVEVDPALRVKREDGGVFDDIYAAGDCVADWCPITGERVPSQLGTVAVRQGKIAGKNAAGGPRATWMGTLNTAVIRVFDLEAAGAGLTQTRAEELGLEVVSATVETTTRARYYPGGEPIAVKLIADADTHRIVGVQSVGGERVRERVDGVALAIRLAAKVEDLLSWDYSYSPPVARVIEPIYEAAELLREELHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX25</accession>
    <name>Q8TX25_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-15 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02066">
        <property type="protein sequence ID" value="AAM02066"/>
        <property type="gene ID" value="MK0853"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0853"/>
    <dbReference type="HOGENOM" id="CLU_1280807_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="215" mass="22901" checksum="6D22C6B7C4F82733" modified="2002-06-01" version="1">MILCLALLILTSPYLAQMILQYNTPYGLKAGTLVVVSGGSSVGETSSLTHTHSLKACVGKVMNYVIGPIPTKYHYGSVLSKFMPTWAAVIFTAAVIASYVILAILSPLAVFHERGSAAESLLLSWAGFLVVLGAVDGGFMCKPMVIGLGMLIVWALGPLPVRRYVIIAVLIGLTPMLLQATIRVVWYLTSPLAPNSVVWAVALLLMTAIARGQGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX39</accession>
    <name>Q8TX39_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0838"/>
    <dbReference type="EnsemblBacteria" id="AAM02051">
        <property type="protein sequence ID" value="AAM02051"/>
        <property type="gene ID" value="MK0838"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0838"/>
    <dbReference type="HOGENOM" id="CLU_965090_0_0_2"/>
    <dbReference type="OMA" id="WSKYLWG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012030">
        <property type="entry name" value="UCP006563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006563">
        <property type="entry name" value="UCP006563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_ligase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="288" mass="31522" checksum="2AE9B76BA2B02B20" modified="2002-06-01" version="1">MSGTQYRLIPVKSRYWKVGSGAVENALEALRGIELKDGDVLLLSEKAVAVAEGELIDERGYEPGVLARLLVILWMRVVWGRILGPLLGPLMDPPMRRETIENLRNYPLKDGSRHKQMILEEFGLLHALEPVSEAGCDVGNVPGYYAAPVPESADRVARELREELRRRRGVDVSVIVGDTDKTYEILGILFTTVARAHPDIVTGTGVIGFLVGRLLAKTVGPTPIAIAGDVSVREAIKLLDMAEDVRTKGAGRTVYDALEEVGDPESLDEEFLNGFEHVPIVIARPVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX66</accession>
    <name>Q8TX66_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ni2+-binding GTPase involved in regulation of expression and maturation of hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hypB_1</name>
        <name evidence="2" type="ordered locus">MK0810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0810"/>
    <dbReference type="EnsemblBacteria" id="AAM02023">
        <property type="protein sequence ID" value="AAM02023"/>
        <property type="gene ID" value="MK0810"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0810"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.852"/>
    <dbReference type="HOGENOM" id="CLU_1193419_0_0_2"/>
    <dbReference type="OMA" id="DMCPDHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="1">
        <location>
            <begin position="4"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="236" mass="25840" checksum="7C13B9948271C120" modified="2002-06-01" version="1">MKLVIVSGTPGSGKTAVILHALERLLDQYNPAVVKVDCLRTDDHEVYRERLGIPATDALAKDMCPDHFAAYNLEHMVRWAEEHGADLLVVETAGLCLRCAPYVDACLGVCVADVTLGPNSPAKVGPFLQTADVVCVNKGDLVSQAEREVYVRKVAEVNPDCRVIETNGLTGAGCELLARLIEEEAPELPDTLDEVRLREKAPLAVCTLCVGETRVAERYHRGVLRRIDGFTEYRGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX72</accession>
    <name>TRM56_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0803</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX72"/>
    <dbReference type="SMR" id="Q8TX72"/>
    <dbReference type="STRING" id="190192.MK0803"/>
    <dbReference type="EnsemblBacteria" id="AAM02017">
        <property type="protein sequence ID" value="AAM02017"/>
        <property type="gene ID" value="MK0803"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0803"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.844"/>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="OMA" id="VVHLTMY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="PTHR42197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000146930" description="tRNA (cytidine(56)-2'-O)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <sequence length="178" mass="20124" checksum="F22DCB99C9229ECB" modified="2002-06-01" version="1">MELEPRTVVLRLGHRRERDKRITTHVCLTARAFGAAGVLISGDHDESVIESVEDVVERWGGPFTVQWVGNWRRVIKDWKRSGGSVVHLTMYGLHIDDVIGELRGENELLVIVGAGKVPAEVFELSDYNVAIGHQPHSEVAALAVFLDRLYEGKELHREFERARLRVVPSEKGKKVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX74</accession>
    <name>Q8TX74_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase related to nitrite reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0801</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02015">
        <property type="protein sequence ID" value="AAM02015"/>
        <property type="gene ID" value="MK0801"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0801"/>
    <dbReference type="HOGENOM" id="CLU_1346413_0_0_2"/>
    <dbReference type="OMA" id="RNACGSV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIR_SIR_ferr" evidence="4">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="3">
        <location>
            <begin position="76"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="203" mass="22367" checksum="F016D4156A71C069" modified="2002-06-01" version="1">MIQSPDGTYVVRLALPPGFVKAEILRTVAELAERYASGEVHITVRQGLEIPEVPPSKLDDLLRKLRELGLEPGSTGPRVRQVTCCPGTRTCANALTDPVPLARKLHEEFLDVWVPAKVKIAVSGCPRGCTRPSENDLGLVAVGGDEWELLVGGYRVARLPEQDVIDAVELTLEWYASEAPPGTRLHRFVTDRVSELRDVLERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX87</accession>
    <name>Q8TX87_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fe-S oxidoreductase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0788</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0788"/>
    <dbReference type="EnsemblBacteria" id="AAM02002">
        <property type="protein sequence ID" value="AAM02002"/>
        <property type="gene ID" value="MK0788"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0788"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.830"/>
    <dbReference type="HOGENOM" id="CLU_042889_0_0_2"/>
    <dbReference type="OMA" id="AHYFMPL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023980">
        <property type="entry name" value="CHP04013_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04013">
        <property type="entry name" value="B12_SAM_MJ_1487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B12-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="147"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="402" mass="45233" checksum="D06AE89B3B9A300E" modified="2002-06-01" version="1">MRRIWFHLDSKNRYSLAHVLGAVEARAPELIPYIEVSRNPDPEPGDIVVFSFNTFMAPEVFEVVERLHDDVVKLAGGPHPSARPDQCLEHGFDIVLIGEGEEVVPEVLRELIRGKEPEERPGVYLGEGNPQPAPRVEDLDRFPPYSEGFRIFCSVEITRGCPWGCAFCQVTRLFGPKLRHRSVEDVVRWVRRGVERYGHTFARFVAPDALAYGSPDGIRLKPDRVKRLLRSLRSIEGLEKVFFGSFPAELRPDSIAKGDAIELIAHYADNERVNIGAQSGSERVLKRIERGHTVEDVEVAVEKALEVGLKPVVDFIFGLPGETEEDQLASVELARWIIKRGGEVRLHYFMPLPGTPLENEEPAPLSSKIRRILGRWTQEGKAEGAWGHQMRLSKTAMKVLSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXB7</accession>
    <name>SYE_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">MK0757</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXB7"/>
    <dbReference type="SMR" id="Q8TXB7"/>
    <dbReference type="STRING" id="190192.MK0757"/>
    <dbReference type="EnsemblBacteria" id="AAM01971">
        <property type="protein sequence ID" value="AAM01971"/>
        <property type="gene ID" value="MK0757"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0757"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.797"/>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="OMA" id="MRFAPNP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119718" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="571" mass="65975" checksum="179C1C850B9B4308" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC5</accession>
    <name>Q8TXC5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea, possible membrane metallohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01963">
        <property type="protein sequence ID" value="AAM01963"/>
        <property type="gene ID" value="MK0749"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0749"/>
    <dbReference type="HOGENOM" id="CLU_772958_0_0_2"/>
    <dbReference type="OMA" id="SHHEFTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="369" mass="41672" checksum="11EC79AE256B542E" modified="2002-06-01" version="1">MYVVPYNTGEALLDFFVITHLTIPFALSYSSVTVQRVLSLSLFTLGVLALLFTVDAFSLFLPVSRHEFTHPFGPLALAAWATSRYLLQLLETRGITAARRTRPYFNLLWVYVGMVGGIMHRDFLLSWFLCWALTEHFIAKYLKVESRFRAFAARAAKMSKRSIVMATLMAIGFLGLLELLAYVLNKPVFSPTLRIKRFAEYTVPGLEFVAKNTHIIGHSTKALPAGYEWRGFGDGFVTLPVGYLMMFRLDVPTLHGALVVKKDLLDYMLPGLFTWAFDFGYIGAVLLSIWVAATLYVGSRCLSEYIKLRRRRMTARFLAREAMLFGCLIAFSVQSLIGVFLFSRAMNSFALATFTLLSALIWAHLVRGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC8</accession>
    <name>HEM3_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable porphobilinogen deaminase</fullName>
            <shortName evidence="1">PBG</shortName>
            <ecNumber evidence="1">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="1">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemC</name>
        <name type="ordered locus">MK0746</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMBS family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM01960" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01960.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXC8"/>
    <dbReference type="SMR" id="Q8TXC8"/>
    <dbReference type="STRING" id="190192.MK0746"/>
    <dbReference type="EnsemblBacteria" id="AAM01960">
        <property type="protein sequence ID" value="AAM01960"/>
        <property type="gene ID" value="MK0746"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0746"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.786"/>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143025" description="Probable porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="298" mass="33053" checksum="55BBAAADCF512C20" modified="2007-09-11" version="2">MSETIRVATRSSRLAIIQTREVIELLERESPRDVEVEIVKTKSRGDVVRDRPLHKLGEKGVFVKEVDRLVLEGKADIAVHSAKDVPSVVDYPVDVAAVPPRRDPRECLVSRHGGLKELPRGATVGTSSPRRRAQILLERPDLKVEPMRGNVDTRVSKVRRREYDAAVLAKVGLDRLGMTSEVSEVYDPEEFVPPAGQGALMITCRKDDDRVKRLLEVVDDEKSRVEVETEKAVVRELGVGCSEPVGVHVRARDGDHVRLVLGLFEEDGSCGHVLKMRGSPEDVVREAVSQAREVLSDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD5</accession>
    <name>IF5A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif5a</name>
        <name type="ordered locus">MK0739</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXD5"/>
    <dbReference type="SMR" id="Q8TXD5"/>
    <dbReference type="STRING" id="190192.MK0739"/>
    <dbReference type="EnsemblBacteria" id="AAM01953">
        <property type="protein sequence ID" value="AAM01953"/>
        <property type="gene ID" value="MK0739"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0739"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.779"/>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="OMA" id="QIMDMET"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="Transl_elong_IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="PTHR11673"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0385">Hypusine</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142494" description="Translation initiation factor 5A">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="1">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <sequence length="136" mass="15179" checksum="15076E3472183859" modified="2002-06-01" version="1">MDVAVKKVEVRQLKKGKYIMIDDEPCKIVEYTTSSPGKHGSAKARIVAVGLFDGKKRTLTKPVDAKVDVPVIERKTAQVVSDMGDTVQLMDMETYETFEVQKPEDEELASQLEPGTMVEYMEAAGKRKIVGIKEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE4</accession>
    <name>Q8TXE4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pyruvate:ferredoxin oxidoreductase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">porG_2</name>
        <name evidence="3" type="ordered locus">MK0730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0730"/>
    <dbReference type="EnsemblBacteria" id="AAM01944">
        <property type="protein sequence ID" value="AAM01944"/>
        <property type="gene ID" value="MK0730"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0730"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.770"/>
    <dbReference type="HOGENOM" id="CLU_087284_0_0_2"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POR" evidence="2">
        <location>
            <begin position="11"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="176" mass="19075" checksum="A62E9A9BF09FBB39" modified="2002-06-01" version="1">MRKEIRISGFGGQGIVLAGVVLGRAAAVYEGYNAVQTQSYGPEARGGASRSDVIVSDEEVMYPYVRRPDFLVTMSQEAYEKYVGSVPEDGLVVYDSTLVEPSREDVEHVGVPATELAEEELGLRIVANMVILGALRELTGIVSFDSLRKAVEDSVPPGTEDVNVRALKLGAREVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXE9</accession>
    <name>Q8TXE9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S-cluster-containing hydrogenase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hycB</name>
        <name evidence="2" type="ordered locus">MK0725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0725"/>
    <dbReference type="EnsemblBacteria" id="AAM01939">
        <property type="protein sequence ID" value="AAM01939"/>
        <property type="gene ID" value="MK0725"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0725"/>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="OMA" id="ILCRLCM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="167" mass="18042" checksum="F1BFD682D67FBDBA" modified="2002-06-01" version="1">MPDVFVLVVAPDRCTDCRKCVNACERTHGHARIYKISEDAPPVTCLQCDDAPCANVCPVNAIVEEGDSWIVTEDCVGCGLCAVACPFGAIEMVNGRADKCTLCVDAPKKVPACVEACDRGALKLVSKSQVAEEKRERFAIEMERIYRTLAKLESEEGSLRGESGSRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF9</accession>
    <name>ALBA1_METKA</name>
    <protein>
        <recommendedName>
            <fullName>DNA/RNA-binding protein Alba 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">albA1</name>
        <name type="ordered locus">MK0715</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXF9"/>
    <dbReference type="SMR" id="Q8TXF9"/>
    <dbReference type="STRING" id="190192.MK0715"/>
    <dbReference type="EnsemblBacteria" id="AAM01929">
        <property type="protein sequence ID" value="AAM01929"/>
        <property type="gene ID" value="MK0715"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0715"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.756"/>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OMA" id="QFNEGAK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="SSF82704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00285">
        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000151699" description="DNA/RNA-binding protein Alba 1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10381" checksum="0D47E616FD2C3222" modified="2002-06-01" version="1">MAEEENVVYVGSKPVMNYVLACITQFNEGANEVRIKARGRAISRAVDVAEIVRNRFMPEVEVKDIKIGTEELETEEGDTVNVSTIEIVLEKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH4</accession>
    <name>Q8TXH4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01914">
        <property type="protein sequence ID" value="AAM01914"/>
        <property type="gene ID" value="MK0700"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0700"/>
    <dbReference type="HOGENOM" id="CLU_3003151_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="56" mass="5742" checksum="CA81F7D8977BE76D" modified="2002-06-01" version="1">MSVEWTILLMTVIAAVFALGIALNASNSGFVSTTGSGWAAGMKELGSEVVRRAATP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT2</accession>
    <name>RIBL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD synthase</fullName>
            <ecNumber evidence="1">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribL</name>
        <name type="ordered locus">MK0578</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXT2"/>
    <dbReference type="SMR" id="Q8TXT2"/>
    <dbReference type="STRING" id="190192.MK0578"/>
    <dbReference type="EnsemblBacteria" id="AAM01793">
        <property type="protein sequence ID" value="AAM01793"/>
        <property type="gene ID" value="MK0578"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0578"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.613"/>
    <dbReference type="HOGENOM" id="CLU_034585_2_1_2"/>
    <dbReference type="OMA" id="FAKKHAD"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000406265" description="FAD synthase">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <sequence length="150" mass="17107" checksum="4E8C5D1817051216" modified="2002-06-01" version="1">MGKRVLAGGVFDILHPGHVAFLEEARKIAGKNGELVVVVARDETVRRLKRTPIVPEEQRVRMVSALKPVDRAILGHPRDFSITLKTVKPDVVVLGPDQDIDEKEVERWAERAGVDCEVRRIEKYERCPLDSTIKIVKRVIELWKRGELRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT3</accession>
    <name>Q8TXT3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted nucleic-acid-binding protein containing an archaeal-type C2H2 Zn-finger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0577"/>
    <dbReference type="EnsemblBacteria" id="AAM01792">
        <property type="protein sequence ID" value="AAM01792"/>
        <property type="gene ID" value="MK0577"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0577"/>
    <dbReference type="HOGENOM" id="CLU_203610_0_0_2"/>
    <dbReference type="OMA" id="NKAHGWL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="69" mass="8386" checksum="09AB4D56DCDF5D12" modified="2002-06-01" version="1">MSEERRPFKAEKVETRDGEILLRCPRCDALFRDQKSYSRHVNKSHLYKKNRPKRILKKMKRRGDVLVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXW9</accession>
    <name>Q8TXW9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01755">
        <property type="protein sequence ID" value="AAM01755"/>
        <property type="gene ID" value="MK0540"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0540"/>
    <dbReference type="HOGENOM" id="CLU_1700290_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="154" mass="17507" checksum="894CFDBCB1E8A879" modified="2002-06-01" version="1">MIKPRDFLRYFLYSYIVTSAQLLGDKAPTLRRMVCKNVVKALLREHPELKDAEPVELVKDVAESFLGADVRIEEKSTETVVHVRGCKICPRDLIEEFTGENPDLEGPVFSYNVCAFVTMVEEILHALGHIDSDIKHDPIKRGRCRVIVRPKGTD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX3</accession>
    <accession>Q9P9A2</accession>
    <name>COOS_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Carbon monoxide dehydrogenase</fullName>
            <shortName>CODH</shortName>
            <ecNumber evidence="1">1.2.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cooS</name>
        <name type="ordered locus">MK0536</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1999" name="J. Microbiol. Biotechnol." volume="9" first="404" last="413">
            <title>Random sequence analysis of the genomic DNA of Methanopyrus kandleri and molecular cloning of the gene encoding a homolog of the catalytic subunit of carbon monoxide dehydrogenase.</title>
            <authorList>
                <person name="Shin H.-S."/>
                <person name="Ryu J.-R."/>
                <person name="Han Y.-S."/>
                <person name="Choi Y.-J."/>
                <person name="Yu Y.G."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 3 [4Fe-4S] clusters per homodimer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="1">Binds 2 [Ni-4Fe-5S] clusters per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">This protein lacks the conserved Cys in position 54; it is replaced by a Trp. It is therefore possible that the D-cluster is either altered or missing in this protein, which may not form homodimers.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAF86272" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="1"/>
    <dbReference type="EMBL" id="AF272857">
        <property type="protein sequence ID" value="AAF86272.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXX3"/>
    <dbReference type="SMR" id="Q8TXX3"/>
    <dbReference type="STRING" id="190192.MK0536"/>
    <dbReference type="EnsemblBacteria" id="AAM01751">
        <property type="protein sequence ID" value="AAM01751"/>
        <property type="gene ID" value="MK0536"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0536"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.571"/>
    <dbReference type="HOGENOM" id="CLU_030631_0_0_2"/>
    <dbReference type="OMA" id="PEYMEQK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01915">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016101">
        <property type="entry name" value="CO_DH_a-bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010047">
        <property type="entry name" value="CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005023">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01702">
        <property type="entry name" value="CO_DH_cata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000157144" description="Carbon monoxide dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9F8A8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="638" mass="69512" checksum="B3A90308E67768C2" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXX5</accession>
    <name>Q8TXX5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0534"/>
    <dbReference type="EnsemblBacteria" id="AAM01749">
        <property type="protein sequence ID" value="AAM01749"/>
        <property type="gene ID" value="MK0534"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0534"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.569"/>
    <dbReference type="HOGENOM" id="CLU_077076_0_0_2"/>
    <dbReference type="OMA" id="HHSYPCT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="264" mass="29102" checksum="57AF918B488E68EB" modified="2002-06-01" version="1">MRTNVYPRGCEDRLRFAALYSHQFGKRMVANLLNEPGHCRACGPVCDGCKYDMYSLVDSLVAVEELPKPEELPPFVDEPEDHLPELPPVDVLVAIGLHPDLLVALSEVYEIKALIVPVEEPDWIDPWIEEKLREVCEENSVELTVARPGCDLEPSGPVTEAFCDAGMIGRPKLFMKVEDGVVIDVHVVRSAPCGCTWFVAKRLVGVDADPEEVKATVSEAHHSYPCTASMEVDKHVGDTLLHVAGRLHIEAALRALEEATDTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY24</accession>
    <name>Q8TY24_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Predicted transcriptional regulators, consists of a molybdenum cofactor biosynthesis enzyme fused to a HTH DNA-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0482</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0482"/>
    <dbReference type="EnsemblBacteria" id="AAM01697">
        <property type="protein sequence ID" value="AAM01697"/>
        <property type="gene ID" value="MK0482"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0482"/>
    <dbReference type="HOGENOM" id="CLU_709060_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="74"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="389" mass="44248" checksum="A1309CA83995B252" modified="2002-06-01" version="1">MDEVSGNRGLISKTVNDLRDEGLIEPTHRGFALTEEGFLELVRPNTDYEYRNEPVRLSTDGPEVEAFLRVITGRSKTRTGSIVATTRCDMRCKFCYYNLVRDHVELTPEEILREAEKRVKAGNREVVIQGASPHTLDTDLVEAVELIKLELGVDVGVGTGPLIPLDLLEDLQRAGLDYLKLSLSGRTPEEWEAITGRRRGFEEFWRVVRWCHEHGLEVTFVGGVVGLPGVPPEADAERILSILALNPRKRIVQFNPAQDPARGPLSPLDRLELIYKTVRSKLPEDVLVKSCCISPMTWSPLYDIRRYLDKVVCMLGIECREYDGCPFTGRVLEQRIMNELYEKGSVSTEELARRLRIARSRLKRRLESMESRGLIRRTESGWTIARRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY27</accession>
    <name>Q8TY27_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0479</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01694">
        <property type="protein sequence ID" value="AAM01694"/>
        <property type="gene ID" value="MK0479"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0479"/>
    <dbReference type="HOGENOM" id="CLU_611954_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="272"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="447" mass="50513" checksum="9FBAF0ACCAF1356A" modified="2002-06-01" version="1">MDPNHVKDEVGQDPSDYTASLEQAQFVVEGIERRGKFRNPLLSTVQANDMIKSIPNLSDDQFRQQVRNLLRSTMGELEEWAKDVGQDVVRRVLEELKRAEEHGYYVPEELKERMEELLQDLQEGPEEREAEGDGEGPSAGASESPSFVSEEGGGGEPTEGESGPTPTESASGVGKGGDPAFTIDRSFPERKKFLKILQSESLKILDEGHEISREFEDVEEGEAKAHFGWLYGFDKADFLSDVDWERSLEEGYFKNVPLTLYRREFRNKDEPKVAILLDSSGSMSGDKMEVAATLAAALFETVGIENIGLWAFRSEVHQLKDFEEVINRRKLIEKILGIPAGGGTDPVKPLIKVLDSLENVDYDKCKIIYITDAIFMHDDFIRIRNLLSDRDDVELYALLIKDEFEHTGPTIFKRITEEFDGGVVQVNPRDREGVREKLRQFVDMISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY35</accession>
    <name>Q8TY35_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaG</name>
        <name evidence="2" type="ordered locus">MK0471</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0471"/>
    <dbReference type="EnsemblBacteria" id="AAM01686">
        <property type="protein sequence ID" value="AAM01686"/>
        <property type="gene ID" value="MK0471"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0471"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.501"/>
    <dbReference type="HOGENOM" id="CLU_1369520_0_0_2"/>
    <dbReference type="OMA" id="NMILLTD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019212">
        <property type="entry name" value="DUF2105_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011311">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09878">
        <property type="entry name" value="DUF2105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019136">
        <property type="entry name" value="EhaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="225" mass="23836" checksum="57FC31F91070DABB" modified="2002-06-01" version="1">MIVPHVVPEITVKMYHVAVAAGIAVGLIAAVDIAADRNPLNRLTMTDALEVGSLTLLASVGTDLAECLILPGLVVGLAELIATAEVLVARYTDGDSLPEFEPLDMEVLRTAPTAIMIGLVVYGVLLTGFTGGAVAGSGAAFYLFSRANRPHYEEWRGMESISGIGWAFWVAGFSTFFLHPKAWLLALILAGTGGILVKVGPKLTLLGYAMGYDVRAGARRRVPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY61</accession>
    <name>Q8TY61_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0445"/>
    <dbReference type="EnsemblBacteria" id="AAM01660">
        <property type="protein sequence ID" value="AAM01660"/>
        <property type="gene ID" value="MK0445"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0445"/>
    <dbReference type="HOGENOM" id="CLU_069296_0_0_2"/>
    <dbReference type="OMA" id="LTYAWDR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010581">
        <property type="entry name" value="DUF1152"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06626">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029122">
        <property type="entry name" value="DUF1152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="34924" checksum="A07DB95CEEF44E8B" modified="2002-06-01" version="1">MYLAEVAGIEELSAVAVLGIGGGGDVVGACHTYRWIREGIEPERLVLGGLTWERAVVDPEPGPRSRDEIVGDVEWIHERIGILRGRARPRRGQEFAESRVRRVLRRMGHRDVEIVLVDVSGGVKGTVDGLKALIEHFELDVVFGIDVGGDALARGDEPGVESPLADSIMTCSLSKLEETVLGVFGWGSDGELTREELRRRFSEIAAEGGYLGAIGLTGRDVKFLKKLAEVVETEASLIPLRAAVEGELGPLEIRGGYRTVELGPASVCTFYFDPEVVARGSILCELVDGTESVEEAHERIRKELGIKTELDWERERAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY82</accession>
    <name>Q8TY82_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">MK0423</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01638">
        <property type="protein sequence ID" value="AAM01638"/>
        <property type="gene ID" value="MK0423"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0423"/>
    <dbReference type="HOGENOM" id="CLU_1615316_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="164" mass="17740" checksum="CCDD8DB6EBD11B57" modified="2002-06-01" version="1">MDLRYLTSAISVSLFVYSGIDTEPLQYVTAQWVESVLHLMGLTVPKAGYTFQVGTVSAMIVKGCVVWPAISLFVGLIVATPGPSVLRKIAALAASVALLTAGNVLRLASMFYMMEVWGVGFRLAHDVIGQLVGLAIVILAAWVAFYIVPETEDKLREIIPWPGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB7</accession>
    <name>PSA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmA</name>
        <name type="ordered locus">MK0385</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYB7"/>
    <dbReference type="SMR" id="Q8TYB7"/>
    <dbReference type="STRING" id="190192.MK0385"/>
    <dbReference type="EnsemblBacteria" id="AAM01600">
        <property type="protein sequence ID" value="AAM01600"/>
        <property type="gene ID" value="MK0385"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0385"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.411"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="OMA" id="FQVEYAR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124175" description="Proteasome subunit alpha">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <sequence length="246" mass="27287" checksum="2ED918A1BFADCCD8" modified="2002-06-01" version="1">MAVQPAQTAYDRAITVFSPDGRLFQVEYAREAVKRGTTALGIKVEEGVVLGVDKRVTSKLIEPESIEKVYQIDTHIGAATAGLVADARVLVERARIEAQTYRYTYGEPIDVDVLVKAICDLKQVYTQHGGVRPFGTALLIAGVDTKGCRLFETDPSGALTEHKATAIGEGRQEALDVFEEEYREDMTLQEAIELAVRALYEASREETTADNLEIAVVDKQGFRKLERKKIEEMFERVVGSEEDEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYE0</accession>
    <name>Q8TYE0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucyl aminopeptidase (Aminopeptidase T)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ampS</name>
        <name evidence="2" type="ordered locus">MK0360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01575">
        <property type="protein sequence ID" value="AAM01575"/>
        <property type="gene ID" value="MK0360"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0360"/>
    <dbReference type="HOGENOM" id="CLU_062630_0_0_2"/>
    <dbReference type="OMA" id="GTMRPHG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000787">
        <property type="entry name" value="Peptidase_M29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02073">
        <property type="entry name" value="Peptidase_M29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="313" mass="34116" checksum="C108C332986020C1" modified="2002-06-01" version="1">MVGLGWAERVVRECLRLSEGERFLVLTDPEMEELGRELFEAAEGTDRALVVVDPRETHGEEPPDHVAAMMRSSDAVAAVTSWSISHTEARRRACEAGARVASMPELTREIAERAIDVDYEELRRLSRELAELLTEASEVRLVTPAGELVMDIGGREALADDGNLRDPGEFGNLPAGEAFVAPIEGSAEGCVRIDGSVAPDGILDEPLELEISEGRVVEVSREDLEFVRLIRRIENGEMLCELGVGTNPGAKLCGVVLEDEKVYGTVHMGFGDNSTFSGRVSSRVHLDAVIKEFELYLDDELVARSGELLGVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYK1</accession>
    <name>Q8TYK1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted diguamylate cyclase, diverged member of the GGDEF superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01513">
        <property type="protein sequence ID" value="AAM01513"/>
        <property type="gene ID" value="MK0296"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0296"/>
    <dbReference type="HOGENOM" id="CLU_1811524_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="144" mass="15999" checksum="CA78D88C648C9198" modified="2002-06-01" version="1">MRSVAVAGDLDRFGEVVEERGWPEYSPNPATGLLTELVERFLTKFPGAVAVKGPDRERGTEEFVVEIPGGDAFLDEILEECERIRRRFEREFKGEVTISLGVGVGPAPSDRRSFREAESPAVRRALEALREAKRRGGNTIVVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN9</accession>
    <name>Q8TYN9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted HD superfamily hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01471">
        <property type="protein sequence ID" value="AAM01471"/>
        <property type="gene ID" value="MK0254"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0254"/>
    <dbReference type="HOGENOM" id="CLU_1559496_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDc" evidence="1">
        <location>
            <begin position="37"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="19545" checksum="2F2D71111AF53F05" modified="2002-06-01" version="1">MESKHLRFLRVLEEVVSDLPERVLRRLCRLHDIRGHRGDTRLEHSLKVAYLCWRLSGKFRVDGKIALESGLLHDIGYGIPRCPLCKLDPGGHCGICHWRTGSELLEEVNVDPAVVRAVRRHMFPYGPPPRTPLDWCVWTSDKLESVLSFLGFRVLPDPVLKRAVSAIYTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYP9</accession>
    <name>Q8TYP9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase, related to NifB/MoaA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01460">
        <property type="protein sequence ID" value="AAM01460"/>
        <property type="gene ID" value="MK0243"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0243"/>
    <dbReference type="HOGENOM" id="CLU_736956_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007549">
        <property type="entry name" value="DUF512"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014494">
        <property type="entry name" value="Fe-S_OxRdtase_NifB-like_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04459">
        <property type="entry name" value="DUF512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018654">
        <property type="entry name" value="Fe-S_or_NB_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF512" evidence="1">
        <location>
            <begin position="243"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="375" mass="42258" checksum="88EE89D9239B34C9" modified="2002-06-01" version="1">MSTEWVTRHLDYDGEETWLIESHGPPDDPTPPCSPTCRHCYATQLPPDARRHLVIDELSMYPSELVNHPRLEDVVRRARELVESRENLPLKVITSGKFLTGRKLRILLRYVDQLDFHLLSTDPSERAKLTRESVREAARVLELVREAAREVDTVANVVAVPDYNLNSLPRILRNLDEWGLHKCVVIPVGVTRYNREGIRPPTPDEMRFLWEICRRMDRELDLDVVPCDSLVPLEEHLEGLDDLASRCAEALGGVDSRVGLVTGEMFGPVIEELCRATNEKLGREVLEPVIVENRYFGGNIGCAGLLTGEDVLHELEHRDLDVAIVPRISVELGSFIDGVTIFEVSLHAGCEIVVGPERLEDLPDVLVEITHSVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR5</accession>
    <name>Q8TYR5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hypC</name>
        <name evidence="2" type="ordered locus">MK0227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0227"/>
    <dbReference type="EnsemblBacteria" id="AAM01444">
        <property type="protein sequence ID" value="AAM01444"/>
        <property type="gene ID" value="MK0227"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0227"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.228"/>
    <dbReference type="HOGENOM" id="CLU_159381_2_2_2"/>
    <dbReference type="OMA" id="MCLAIPC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="92" mass="10327" checksum="3FA883535FFBE670" modified="2002-06-01" version="1">MCLAIPGRIVELDGNRAVVDFGGVRQEVDVSLLEDVEEGDWVIVHTGFAIQKLDEEEARASLEIWEEVLKHIEEELENDTSGVREKARPSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR9</accession>
    <name>Q8TYR9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pepP</name>
        <name evidence="5" type="ordered locus">MK0223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0223"/>
    <dbReference type="EnsemblBacteria" id="AAM01440">
        <property type="protein sequence ID" value="AAM01440"/>
        <property type="gene ID" value="MK0223"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0223"/>
    <dbReference type="HOGENOM" id="CLU_848908_0_0_2"/>
    <dbReference type="OMA" id="NVEWITH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="131"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="37113" checksum="3ED8575E2CEC832A" modified="2002-06-01" version="1">MKSDDFFLEKVEKLEELAEGEPCALVRRQNVEWITHFPGLAFHADFESEEYTLVVHRMDSRAVREWPIPETVEVITHDEANRLTPARAVDDESAARKVPCYRVKNVNEDVTRARLSKSKSELRFIEELVEATERILVKVLPPEDSEAEVASDLIREATIRGFQTAFDPIVAYDEGAGVPHHRPSPEEKTWTKCALVDYGIKMVYCTDITRTVVSENEAGDVLEVVVNALEEALRELQAGVNPKELEEELRGWMEDEAPGFEFPHSLGHHVGVTVHEGRLMGRLPEGAVITVEPGLYSDEFGIRVEEMVVVGKRKCRTLTKLPRVWER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT9</accession>
    <name>PGP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <shortName evidence="1">PGP</shortName>
            <shortName evidence="1">PGPase</shortName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0203</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of 2-phosphoglycolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phosphoglycolate + H2O = glycolate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14369"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="EC" id="3.1.3.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal SPP-like hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYT9"/>
    <dbReference type="SMR" id="Q8TYT9"/>
    <dbReference type="STRING" id="190192.MK0203"/>
    <dbReference type="EnsemblBacteria" id="AAM01420">
        <property type="protein sequence ID" value="AAM01420"/>
        <property type="gene ID" value="MK0203"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0203"/>
    <dbReference type="HOGENOM" id="CLU_044146_2_0_2"/>
    <dbReference type="OMA" id="DTGYAYH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01419">
        <property type="entry name" value="GPH_hydrolase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006382">
        <property type="entry name" value="PGPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146718" description="Phosphoglycolate phosphatase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01419"/>
        </source>
    </evidence>
    <sequence length="232" mass="25162" checksum="BAF79060B3483660" modified="2002-06-01" version="1">MTSLVITDIDGTITGDDRAVHLKCIRYLRELQKRGIPVGIATGNTLCYSRSAATLLGFEGPLIAENGGIVAVDDEEISTVPEEDIELIQEAYRELRRRLGVRRTEPPGLRRTEVAIYRDVPIEEVERVLDGLGYSGRIEVVDTGFAYHLKSKRVDKGKGLLVICERLGIDPDDVVAIGDGDNDAPLLKAAGLGVAPANATENVKRIADVVLDAENGEGVATFLRKLLEEVDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV1</accession>
    <name>CAPPA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoenolpyruvate carboxylase</fullName>
            <shortName evidence="1">PEPC</shortName>
            <shortName evidence="1">PEPCase</shortName>
            <ecNumber evidence="1">4.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppcA</name>
        <name type="ordered locus">MK0190</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>oxaloacetate + phosphate = hydrogencarbonate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:28370"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PEPCase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.31" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYV1"/>
    <dbReference type="SMR" id="Q8TYV1"/>
    <dbReference type="STRING" id="190192.MK0190"/>
    <dbReference type="EnsemblBacteria" id="AAM01407">
        <property type="protein sequence ID" value="AAM01407"/>
        <property type="gene ID" value="MK0190"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0190"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.190"/>
    <dbReference type="HOGENOM" id="CLU_517433_0_0_2"/>
    <dbReference type="OMA" id="QSAFRYD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008964">
        <property type="term" value="F:phosphoenolpyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01904">
        <property type="entry name" value="PEPcase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007566">
        <property type="entry name" value="PEP_COase_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14010">
        <property type="entry name" value="PEPcase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006677">
        <property type="entry name" value="UCP006677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02751">
        <property type="entry name" value="PEPCase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000309601" description="Phosphoenolpyruvate carboxylase">
        <location>
            <begin position="1"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01904"/>
        </source>
    </evidence>
    <sequence length="532" mass="59778" checksum="98E64A0E55D089C9" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV2</accession>
    <name>Q8TYV2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">N2,N2-dimethylguanosine tRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trm1_2</name>
        <name evidence="7" type="ordered locus">MK0189</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0189"/>
    <dbReference type="EnsemblBacteria" id="AAM01406">
        <property type="protein sequence ID" value="AAM01406"/>
        <property type="gene ID" value="MK0189"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0189"/>
    <dbReference type="HOGENOM" id="CLU_948675_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="PTHR10631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 6" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="293" mass="32440" checksum="191B8F8D3AA841C5" modified="2002-06-01" version="1">MRTDGGVKRVAGAEFVLYKEGGSEFYVPDPERYSQDGLPTRDAPVAFAPNAAVNRSVLVLFHRVRPVRRFGDVFCGVGARAIRLHIEAEVEWSVLSDVNPIACQIAMINVRRLGLPSEVRCMDAVAALSTFDFDAVDLDVFGTPIPFAQAAFRCVRDGYVHVTATDLESLYTPAARRKYLLEGPLPRRDTDLEVRAVVGALARLAASVDIGIEPMYCLVEPGVRIRVGLECRRGRSRANETLDMLDYVDGVGPVWGGDLHDEDVVEEMLEELDCTGWSEKHARDVRKSLEVTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV5</accession>
    <name>RNH2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease HII</fullName>
            <shortName evidence="1">RNase HII</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhB</name>
        <name type="ordered locus">MK0186</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYV5"/>
    <dbReference type="SMR" id="Q8TYV5"/>
    <dbReference type="STRING" id="190192.MK0186"/>
    <dbReference type="EnsemblBacteria" id="AAM01403">
        <property type="protein sequence ID" value="AAM01403"/>
        <property type="gene ID" value="MK0186"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0186"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.186"/>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="OMA" id="REECRFF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00729">
        <property type="entry name" value="TIGR00729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000236279" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <sequence length="210" mass="24263" checksum="225D2B2429CE4724" modified="2002-06-01" version="1">MSGVMGIDEAGRGPVFGPMVVAGVLAPKRELGLGARDSKELTRSARRRLIRALMSDERLRVDLRIVWPWEIDEEGVAKAEFEAIRELVRRAMPDEVILDKPGNYSPERLRRELDLPEGINLIAEERADAKYEVVSAASIVAKTYRDWIVRLLELEYGEVGSGYPSDPRTVDRLRRELRRGGELLKYFRRSWETYKRVESEVKQRKLEDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYW1</accession>
    <name>Q8TYW1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin biosynthesis protein CbiM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiM</name>
        <name evidence="8" type="ordered locus">MK0180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01397">
        <property type="protein sequence ID" value="AAM01397"/>
        <property type="gene ID" value="MK0180"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0180"/>
    <dbReference type="HOGENOM" id="CLU_1222496_0_0_2"/>
    <dbReference type="OMA" id="WGLVGAN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAM01397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="190" mass="20322" checksum="F16EA2561BFAD460" modified="2002-06-01" version="1">MIPLWWCAVFYAMAAIPVGLGALRIRSEGRKTILVGLLAGLSFVFMQVPIGHAHVNLTGPIGILLGPWSSAVAVFIVNLACALMGHGGITIVGLNTLINWGEAAGVWALYRLLRERLDYGAAAGIATFSVLATSSVIPSFVMAWVINKPVLPFLLTLTTVWIVTAIIEAVITASMVKALAQMKPDWVRDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-03" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYX9</accession>
    <name>ASD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative archaetidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="1">4.1.1.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Archaetidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Archaetidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">asd</name>
        <name type="ordered locus">MK0162</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of archaetidylethanolamine (PtdEtn) from archaetidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>archaetidylserine + H(+) = archaetidylethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:51488"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:134176"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYX9"/>
    <dbReference type="STRING" id="190192.MK0162"/>
    <dbReference type="EnsemblBacteria" id="AAM01379">
        <property type="protein sequence ID" value="AAM01379"/>
        <property type="gene ID" value="MK0162"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0162"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.162"/>
    <dbReference type="HOGENOM" id="CLU_072492_2_0_2"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000029827" description="Archaetidylserine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000029828" description="Archaetidylserine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="180"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="1">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <sequence length="214" mass="23426" checksum="FC9AC158CDB1FD94" modified="2002-06-01" version="1">MMAPGWWKFVTPPAALGAALFPWSRPLSFLCLGTAAFLAFFFRNPPREPPSDPSLAVSPADGRLLGYVMEAGEASDDELSSYLDDPITVSVFMSPLDVHVNRAPLDGRVVEAEILKGRFRPAFRKDSATENNRAVLLFDGDPPFVVRLVSGAVARRIDLYVQEGDEVNKGEPIGMIRFGSRVDLAVPRSSVEELLVRKGDSVKAGETPVIRVKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-29" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY9</accession>
    <name>SYG_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="1">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyS</name>
        <name type="synonym">grs1</name>
        <name type="ordered locus">MK0152</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYY9"/>
    <dbReference type="SMR" id="Q8TYY9"/>
    <dbReference type="STRING" id="190192.MK0152"/>
    <dbReference type="EnsemblBacteria" id="AAM01369">
        <property type="protein sequence ID" value="AAM01369"/>
        <property type="gene ID" value="MK0152"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0152"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.152"/>
    <dbReference type="HOGENOM" id="CLU_015515_1_0_2"/>
    <dbReference type="OMA" id="EPSYGID"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_A">
        <property type="entry name" value="Gly_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022960">
        <property type="entry name" value="Gly_tRNA_ligase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000072991" description="Glycine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="323"/>
            <end position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="432"/>
            <end position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="436"/>
            <end position="439"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <sequence length="571" mass="66079" checksum="34BF08F8A23E2A6A" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ1</accession>
    <name>Q8TYZ1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0150"/>
    <dbReference type="EnsemblBacteria" id="AAM01367">
        <property type="protein sequence ID" value="AAM01367"/>
        <property type="gene ID" value="MK0150"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0150"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.150"/>
    <dbReference type="HOGENOM" id="CLU_982163_0_0_2"/>
    <dbReference type="OMA" id="QGLCGRI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009181">
        <property type="entry name" value="UCP004929_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09872">
        <property type="entry name" value="DUF2099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004929">
        <property type="entry name" value="UCP004929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03275">
        <property type="entry name" value="methan_mark_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="273" mass="30205" checksum="8DA31C9C7FB0F55A" modified="2002-06-01" version="1">MAKYEHMMECLGKTPVRVIWKDGDVEVRAEGDPMIERCPLMRRREGFSKLTREAAERHVLNKVNEVGMFTPKRRIRSCRRYTPFGVSETLMTCLQHRLIDAAVIVSDCAGTVVTDKPAIVQGLCGEISGIRDTDPIPEVVDRLEDSGCSVLGRIDQREGVEIALEEGRRFVAVTVADAGDAEAIREEFGDDVLIAAVHTTGTDEEDAERLVQYCDIITGCASKAVRRAAGRRYILKVGSRVPVYGITPAGAEALWLNVRELLGNLKLEVRHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYZ9</accession>
    <name>Q8TYZ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted nucleotide-binding protein related to universal stress protein UspA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0142"/>
    <dbReference type="EnsemblBacteria" id="AAM01359">
        <property type="protein sequence ID" value="AAM01359"/>
        <property type="gene ID" value="MK0142"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0142"/>
    <dbReference type="HOGENOM" id="CLU_049301_11_4_2"/>
    <dbReference type="OMA" id="HYAPCTI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="158" mass="17749" checksum="9EA79A44B8C8A68D" modified="2002-06-01" version="1">MPRKILVPFDGSEPAELALKWALLDAHDHGFPIKVMYVVDRSLDLLTGFAPRETVLKELKERGEKILEEAEQIAGELGVDVKIEKKVCVGIPWREIVREAEDDEEINLIVMGSHGRTGPEHAILGSVAENVIRHSPVNVLVVKREKRVEDSVEESSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ02</accession>
    <name>Q8TZ02_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein, PrgY homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01356">
        <property type="protein sequence ID" value="AAM01356"/>
        <property type="gene ID" value="MK0139"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0139"/>
    <dbReference type="HOGENOM" id="CLU_1113871_0_0_2"/>
    <dbReference type="OMA" id="REEFMAN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PTHR21530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="249" mass="27614" checksum="61489891775C0E2B" modified="2002-06-01" version="1">MLMDRFEMFGSELYVLRTAHAGVDGDRVRRKILELDPEAVLVELCEGRLLSFLAELRGERAGSRTGGITGRLVAVAERIVGRVVGGELGEDVKGAIEAALELEAEIVPVDMDISWVFRRMKMKASRWELLKFQFSVAIDVLRSLLRPGQTRDVVLSSVADEEAAREMVQGLRRAFPRIAEVLIDERNRVIAENTIEFLHSREDVTKAVLVIGAAHYGVLDILRDAELESASRHDDEDTASGEGEEETAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ06</accession>
    <name>Q8TZ06_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Terpene cyclase/mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01352">
        <property type="protein sequence ID" value="AAM01352"/>
        <property type="gene ID" value="MK0135"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0135"/>
    <dbReference type="HOGENOM" id="CLU_411422_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="667" mass="74757" checksum="732EB33D12FF7FE8" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ15</accession>
    <name>Q8TZ15_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kef-type K+ transport systems (NAD-binding component fused to domain related to exopolyphosphatase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0126</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0126"/>
    <dbReference type="EnsemblBacteria" id="AAM01343">
        <property type="protein sequence ID" value="AAM01343"/>
        <property type="gene ID" value="MK0126"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0126"/>
    <dbReference type="HOGENOM" id="CLU_046377_1_1_2"/>
    <dbReference type="OMA" id="KCATALM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="8"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="477" mass="52657" checksum="9677C8B167898CF2" modified="2002-06-01" version="1">MGGGSVGHVVADALRDRGEEVVIVDCNEDRAEVLREQGFDVIIGDITEKEVLLKAGIERAVMVYVLTPDDDANAEAIRLIREINEDTYVIARVTDEERVEEFKELGADEVLSPNQLLVEKLLHNIDNVRNRRKVHELLTKLEDVETLAIIPHNNPDPDSIASAVALQEIASIVDVQSDVVYGGEIGHQENKALVNLLDIEMKRISRVDLDEYDAIAVVDTPVLPRELAEYDGIEDRILVAVDHHDSSDGMMDMNGTSKSALELADFVDHRPEVGAASTILTQYLKILDRNVDRRIATALLYGIRTDTLNFTRNVSPEDLKAAAYLYPRADHEALAKIESPDISPETLDVLGEAIRNRTVIRSYLFSNVGFVKNEDALPQAADYLLNLEGVHTVIVFGVVNGKVKISARTDDIRLNIGEIMKEAFGDVGSAGGHSKAAAAEIPLGIFQDVESDMVLDLVEQAVRKRIFKVIGIEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ37</accession>
    <name>Q8TZ37_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holliday junction resolvase Hjc</fullName>
            <shortName evidence="10">Hjc</shortName>
            <ecNumber evidence="10">3.1.21.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">hjc</name>
        <name evidence="11" type="ordered locus">MK0102</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="10"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0102"/>
    <dbReference type="EnsemblBacteria" id="AAM01319">
        <property type="protein sequence ID" value="AAM01319"/>
        <property type="gene ID" value="MK0102"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0102"/>
    <dbReference type="HOGENOM" id="CLU_1850647_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="archeal_HJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="PTHR39651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="138" mass="15750" checksum="63FD98559FD034D3" modified="2002-06-01" version="1">MSYRRGADFERQLVRYLREHGGEAVRVAGSGGAVDVVGYAPAMGHVAVECKVRRDDRLYVEKEEIEGLTTFAERFRAEPLIAWKPPHVRTGLPLNAVLFPPDLMEERERTYVIDLETALEEGIDASRLVTRPLDHYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-01-19" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVI6</accession>
    <name>F1SVI6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted dinucleotide-utilizing enzyme of the ThiF/HesA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0010"/>
    <dbReference type="EnsemblBacteria" id="AAM01227">
        <property type="protein sequence ID" value="AAM01227"/>
        <property type="gene ID" value="MK0010"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0010"/>
    <dbReference type="HOGENOM" id="CLU_1346434_0_0_2"/>
    <dbReference type="OMA" id="DMEPITP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012028">
        <property type="entry name" value="UCP006529_dinclt"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006529">
        <property type="entry name" value="UCP006529_dinclt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="224" mass="24753" checksum="1D31720FE402C7F2" modified="2011-05-03" version="1">MEPDELIEELELRKRPRGTVLLCGIGRLGFRVFLRLLETHRGGPKRIVCVDGQRVEPDDIIHLRHGARIGENKAEFAARLGRVHPLREVHAEPEFVTEENASELVDTWEPDVVVITIAGGRTTPVTAALAREARRFGAVTVSTGGVFGYGSEEVRVVQLEEAEGPVARELREYGAPSDHVLVTTGRYIRDPDPIPPMVLERVADELTELALRALSNPSRGDDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY0</accession>
    <name>Q8TGY0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK1630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1630"/>
    <dbReference type="EnsemblBacteria" id="AAM02843">
        <property type="protein sequence ID" value="AAM02843"/>
        <property type="gene ID" value="MK1630"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1630"/>
    <dbReference type="HOGENOM" id="CLU_005316_0_1_2"/>
    <dbReference type="OMA" id="RVNRQHH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035926">
        <property type="entry name" value="NusB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006027">
        <property type="entry name" value="NusB_RsmB_TIM44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01029">
        <property type="entry name" value="NusB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48013">
        <property type="entry name" value="SSF48013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM_MT_RSMB_NOP" evidence="6">
        <location>
            <begin position="168"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="5">
        <location>
            <begin position="257"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="448" mass="50859" checksum="41BAA3228BF16B37" modified="2002-06-01" version="1">MENQLLAVKALVLVETRGMPIPEAVERTCRDRPIDVRRSVQAFVYETIKRRNLLDELVAAGSDAHPEDVRSPYVRQILRVGTLEMKIWRNPPPAVTDCMVRIAKRLVGSKAGAFVNAVLRGVERVSVKDVLEDRPWTERLALKYGHPEWFVLYVLDLFEGDRSRVELLLRANNRVPPQYLRINRLKLDPLVAGDVLEREYGIVTEPTFLEEVRALVRGRGYGSRAWREGLFDVQDLASASASAALSAEPGETVLDVCAAPGSKTTHTAERMLDEGEVWAVDRSEWGLRVLERRCRRLGITCVRTICRDARGLTVDDLPDVPDRILVDPPCSTTGVWNRNPDSRWKPKPLERFAERQWEILEPALRIAEEHGCTLVYSTCSVSWEENEAIVKRALEEFDVKLVDAGVLGSPGIEEFRGERFSGYKKVRRYWPFRHDTAGFFVAKMKGKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUV8</accession>
    <name>GGR2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglycerophospholipid reductase 2</fullName>
            <shortName evidence="1">DGGGPL reductase 2</shortName>
            <ecNumber evidence="1">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2,3-bis-O-geranylgeranylglyceryl phosphate reductase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranyl reductase 2</fullName>
            <shortName evidence="1">GGR 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1645</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAM02858" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02858.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUV8"/>
    <dbReference type="SMR" id="Q8TUV8"/>
    <dbReference type="STRING" id="190192.MK1645"/>
    <dbReference type="EnsemblBacteria" id="AAM02858">
        <property type="protein sequence ID" value="AAM02858"/>
        <property type="gene ID" value="MK1645"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1645"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1807"/>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="OMA" id="CSCAQYE"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000351459" description="Digeranylgeranylglycerophospholipid reductase 2">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="200"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="286"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="290"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="396" mass="42769" checksum="D72E9633F7961A3B" modified="2008-10-14" version="2">MEFDVVVVGAGPAGSVAAWAAAEAGCDVLILERKAEIGVPKQCAEGISAHGLEHAGIEPQDEWIATEISRALIYAPNGKEFEVPGDGYVLERRVFDKWLVVRAVEAGAEVELLAHARRALLDEGRVVGVEYEGEDGVHEVRARIVIAADGIESRIGRTAGLVPSLKPVEMCTCAQYEMVGVDVEEDATHFFVDAEFFPGGYFWIFPKGEGRANVGLGIRGSESEPGDALKVLNRALEDHELISEAVADAVPVEVNVGGVPVCGPVERTYGDGILLVGDAARQVNPLTGGGLHTSLVCGRIAGEVAAEAIEEDDTSASFLKRYQDRWEEEFGKTFKYALKASKIFSEMSNEELNALAEALDREDILRLVKGEEVVKVAKKVISRKPSLLKYAKHLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW0</accession>
    <name>Q8TUW0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="3">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiA</name>
        <name evidence="3" type="synonym">cfbB</name>
        <name evidence="6" type="synonym">cobB_2</name>
        <name evidence="6" type="ordered locus">MK1643</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP + 2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP + cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="3"/>
    <dbReference type="EC" id="6.3.5.12" evidence="3"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1643"/>
    <dbReference type="EnsemblBacteria" id="AAM02856">
        <property type="protein sequence ID" value="AAM02856"/>
        <property type="gene ID" value="MK1643"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1643"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1805"/>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="OMA" id="QPFKCGP"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="15"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="257"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="3">
        <location>
            <position position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="463" mass="51593" checksum="F729678DA1ADB30D" modified="2002-06-01" version="1">MIHGNRGALLPIPRIVLAGSSSACGKTMITAGIIQALRADGYEVQPFKVGPDYIDPSYHWLASGRPCGNLDTFLFREKHVRWLFEHRCEGADLAVVEGVRGLYEGIGAVGVRGSTYHVSEVLNAPVVLIVDARSLTKSVAALVKGYAELEGANIAGVILNRIRSEVHYHKVRRALVKYTDVKVLGYVPRDRRLKVEYRHLGLVPTPERLEEMRERLRTVAEVISEHVDLDALIDVAEAAGPLGPGERPWEVNPTKCRIAVAKDEAFNFYYPENLEALEENGAKLLEFSPVRDEDVPPDADALYIGGGYPELFARQLEDAESTRNSIRELAESGAPIYAECGGFMYLCRELRWNEDRYRWVGVFDVAVEMTDRVQGLSYTVARAVDDTPVTRKGETFKGHEFHYSRLVRPEGLESAYRIIRGQGWRGREGFRPKDLPNVLGTYVHVHAASHPTFATNFTGSTGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-08-12" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW1</accession>
    <name>Q8TUW1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mannose-6-phosphate isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cpsB_2</name>
        <name evidence="2" type="ordered locus">MK1642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02855">
        <property type="protein sequence ID" value="AAM02855"/>
        <property type="gene ID" value="MK1642"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1642"/>
    <dbReference type="HOGENOM" id="CLU_129810_1_1_2"/>
    <dbReference type="OMA" id="WVENDGS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="37"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="132" mass="14817" checksum="A7F3F0CDF998C2C5" modified="2002-06-01" version="1">MKRSPRDSVPYVTLDGSLIYEVVRPEFSRVNTVSLAVAEIPPGESTVPHYHLDFDEVYWVLEGRGIVHVGSRSLEVHPEDCVEIPRGSVHWVENDGSETLRILCVCSPPYRHETTVTLGSKRTSRSSSPTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ2</accession>
    <name>Q8TUZ2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chorismate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pheA</name>
        <name evidence="2" type="ordered locus">MK1609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1609"/>
    <dbReference type="EnsemblBacteria" id="AAM02822">
        <property type="protein sequence ID" value="AAM02822"/>
        <property type="gene ID" value="MK1609"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1609"/>
    <dbReference type="HOGENOM" id="CLU_131518_3_3_2"/>
    <dbReference type="OMA" id="IRDEKRE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01791">
        <property type="entry name" value="CM_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="92" mass="10623" checksum="D90FCDC0F49BF8EF" modified="2002-06-01" version="1">MNVLEELRREIDRIDECLLDAVIERLKVAREIGRVKAQEGLPLTDEEREKELRERWRKRFKTEGLDPALADIVLASILKVSKEVQRGVIGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUZ8</accession>
    <name>Q8TUZ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02816">
        <property type="protein sequence ID" value="AAM02816"/>
        <property type="gene ID" value="MK1603"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1603"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1766"/>
    <dbReference type="HOGENOM" id="CLU_1472100_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="183" mass="20461" checksum="078A1A5E21D55C4B" modified="2002-06-01" version="1">MRSLSVLITALTIPLGVAGQIMTPEEVSELPYNHPVIVDVVAGPPEADSIEAYTRIVWMDPITHERHIEEEVEVRTSNTLLLNVVVASPHRPATCTLMVSFDDRRVLERTVRLQANGIVQESLELDVTSGRHTLSVEVTDGGTEDIVSCNLRVEPSEGNGGENNQVHPFQGLVLWPRRRRRRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV13</accession>
    <name>Q8TV13_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized Zn-finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02801">
        <property type="protein sequence ID" value="AAM02801"/>
        <property type="gene ID" value="MK1588"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1588"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1749"/>
    <dbReference type="HOGENOM" id="CLU_142653_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="157" mass="18029" checksum="B31F2C35AE69C968" modified="2002-06-01" version="1">MRGNPDIKRSDLNKMAERLLAGHKMLGIHCPECKVPLFQDPKTGTVSCPICGTEFEVVEEEAKKVKRKAKERRKRAESEEKKTKWAEKKPESGKKERKPEKEKQKRRPRLPGSEDVRKAALQVVTARLKRAAETKDPEHALRELEVAEKALEILKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV44</accession>
    <name>Q8TV44_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02769">
        <property type="protein sequence ID" value="AAM02769"/>
        <property type="gene ID" value="MK1556"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1556"/>
    <dbReference type="HOGENOM" id="CLU_1782507_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="145" mass="15778" checksum="E43A722D75EC01F1" modified="2002-06-01" version="1">MIHTTLWIALAGVCLVLAGIALISAIVVEGTIEATTWFTVADLAVCAAAIYVGITGHQFSSLLPTVGLIVATLMAVAHVTYTWIKGILRLVTGRESKSVGERPDTLRAFLTRVLEKLGYKGKVDPGDREAARRVLETCRRWGWDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV72</accession>
    <name>Q8TV72_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdopterin biosynthesis enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moeA</name>
        <name evidence="3" type="ordered locus">MK1524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1524"/>
    <dbReference type="EnsemblBacteria" id="AAM02737">
        <property type="protein sequence ID" value="AAM02737"/>
        <property type="gene ID" value="MK1524"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1524"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1684"/>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="OMA" id="VDHVHPT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="2">
        <location>
            <begin position="189"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="415" mass="45111" checksum="7E4BFA73B4AC37BE" modified="2002-06-01" version="1">MSKRKYRGFVYTDDLAKPEEFIRFLRRFLSPPEPETVEVGPELSGRVLAEDVRAERPSPPFTRSAMDGYAVRSEDAEPGARLKVVGESRIGEGTDVKVGPGEAVYVDTGAPVPEGADAVIPVEFVERDGDEIIVEEGVDPGDNLDPAGCYVEEGEVLYRSGHLVEPVDVGVMLEHGVEEVDVHRPLRVSIVVTGNELVHRPSELESETQVVDCIGPVLTAELENMGCVEVVGTSLVKDDPGEIRETVEDYADRSDVVLITGGSSVGERDHVKDILDDTFDAFIHGIRARPGRPFGIAAREGTVAFTLPGWPTSCYSTFRVWVVPSLLVLAGADPLVVGFETEATGIKSKREVGVIARVRLTSDGAEGLSRDRSSHFALFRETDGFALVPPGGSERALILPLRGFLNLFDEVERKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV82</accession>
    <name>Q8TV82_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted metabolic regulator containing V4R domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02724">
        <property type="protein sequence ID" value="AAM02724"/>
        <property type="gene ID" value="MK1511"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1511"/>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="OMA" id="GTTLCHF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V4R" evidence="1">
        <location>
            <begin position="103"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="166" mass="18855" checksum="8F00912A6400CB99" modified="2002-06-01" version="1">MRMSRERIFREVKESLKNIQEVRSELGDNVSLSTLRYILASMVITLGRGVGSTFYRAGYDIGVYKAKSHDLKGIEEAFEYVEKAFERTGTGIIERWEMKEDGKIEITMRESATAAGYDIGKKLCYYQAGFIAGILHGATGERWEVHETKCMAEGHDHCEFVAIRRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV92</accession>
    <name>MFNA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable L-tyrosine/L-aspartate decarboxylase</fullName>
            <shortName evidence="1">TDC/ADC</shortName>
            <ecNumber evidence="1">4.1.1.11</ecNumber>
            <ecNumber evidence="1">4.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mfnA</name>
        <name type="ordered locus">MK1500</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis. Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + L-tyrosine = CO2 + tyramine</text>
            <dbReference type="Rhea" id="RHEA:14345"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="EC" id="4.1.1.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + L-aspartate = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="1"/>
    <dbReference type="EC" id="4.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TV92"/>
    <dbReference type="SMR" id="Q8TV92"/>
    <dbReference type="STRING" id="190192.MK1500"/>
    <dbReference type="EnsemblBacteria" id="AAM02713">
        <property type="protein sequence ID" value="AAM02713"/>
        <property type="gene ID" value="MK1500"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1500"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1658"/>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_2"/>
    <dbReference type="OMA" id="DPHKMGL"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004837">
        <property type="term" value="F:tyrosine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147024" description="Probable L-tyrosine/L-aspartate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <sequence length="372" mass="40407" checksum="FCB8BAE154F92E17" modified="2002-06-01" version="1">MILQRDSDYSDGTVLGSMCTEPHPVAAEAFVAGLHVNLGDPYLFPNAYRAERECIGWLAETLLDHPAPEEAEGSIVSGGTEANILAAYAAREVTGGREIIVPATRHFSFEKAARMLRMKLVEAPLRSDYTVDVDAVQDLISRDTALIVGIVGTTETGSVDDIEALSDVAEDHGVPLHVDAAFGGFTAPFLREEYPLPRFGFDLEAVVSVTVDPHKMGLVPPPAGGIVFRDDEFPKAIEVYAPYLSGGGASQYTITGTRPGAPVLALYANILELGEEGYRRIAFRCYEETLKVAEKARELGLELAVDPPHLNLVNIRLPDRGTAERLLRESEREGWKISVSTKPLGVRIVMMPHLDAETVSRFLELVARVLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVC9</accession>
    <name>Q8TVC9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted TIM-barrel enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02676">
        <property type="protein sequence ID" value="AAM02676"/>
        <property type="gene ID" value="MK1463"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1463"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1619"/>
    <dbReference type="HOGENOM" id="CLU_075239_1_0_2"/>
    <dbReference type="OMA" id="ENFFDAP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="PTHR21381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="271" mass="29079" checksum="A56E108199D5B191" modified="2002-06-01" version="1">MNRTGGSPGALPLEVVGVVHLPPLPGSPRAKSIEEVVERARRDAARLEDGGVDAVLVENFGDTPYYPDDVPKITVACMTRAVAEVVDTVSVPVGVNVLRNDGVAAVDVCAATGASFIRVNAYVEAVATDQGVLQPVAHMVWREIDRLGVDVEVYADIRVKHGRPLDDRPVEEVARDAVERGLADAVIVTGSATGSPPRPEEVRKVARVVDRVLVGSGVTPENAHVFLRAGAAGFIVGTYFKKNGITENPVDVDRVRELVRFIRRSGVERWP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVE2</accession>
    <name>IPYR_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="1">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="1">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppa</name>
        <name type="ordered locus">MK1450</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVE2"/>
    <dbReference type="SMR" id="Q8TVE2"/>
    <dbReference type="STRING" id="190192.MK1450"/>
    <dbReference type="EnsemblBacteria" id="AAM02663">
        <property type="protein sequence ID" value="AAM02663"/>
        <property type="gene ID" value="MK1450"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1450"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1606"/>
    <dbReference type="HOGENOM" id="CLU_073198_1_1_2"/>
    <dbReference type="OMA" id="DEPTFPG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00209">
        <property type="entry name" value="Inorganic_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="PTHR10286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="SSF50324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137550" description="Inorganic pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00209"/>
        </source>
    </evidence>
    <sequence length="176" mass="20301" checksum="E73BD4832D7104D9" modified="2002-06-01" version="1">MMNLWKDLEPGPNPPDVVYAVIEIPRGSRNKYEYDEERGFFKLDRVLYSPFHYPLDYGFIPRTLYDDGDPLDILVIMQDPTFPGCVIEARPIGLMKMLDDSDQDDKVLAVPTEDPRFKDVKDLDDVPKHLLDEIAHMFSEYKRLEGKETEVLGWEGADAAKEAIVHAIELYEEEHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG1</accession>
    <name>Q8TVG1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Precorrin-6B methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cobL_2</name>
        <name evidence="3" type="ordered locus">MK1428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1428"/>
    <dbReference type="EnsemblBacteria" id="AAM02641">
        <property type="protein sequence ID" value="AAM02641"/>
        <property type="gene ID" value="MK1428"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1428"/>
    <dbReference type="HOGENOM" id="CLU_089162_2_0_2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="212" mass="23352" checksum="802867B9871ACAB4" modified="2002-06-01" version="1">MQVVGVGPNPTRFLTIEAIERIARADLLISSESILREIDGLRVEAEIDLSDKEVVTWNGSVRETLTEYADSDPVVVARGDPTYMGVGRLASLLFDDVEIVPGVSSLQALTARFGRGFHEVEAHVNLHSEEDVEKVVESLGAGRTTAVLFGKVRPAKVVETIESVGLNVKVIAGERLWYPDERLATELHSLRNFSEFTVAIFEPDHVIETSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVH9</accession>
    <name>Q8TVH9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1410"/>
    <dbReference type="EnsemblBacteria" id="AAM02623">
        <property type="protein sequence ID" value="AAM02623"/>
        <property type="gene ID" value="MK1410"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1410"/>
    <dbReference type="HOGENOM" id="CLU_152992_0_0_2"/>
    <dbReference type="OMA" id="TEQTNEF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010382">
        <property type="entry name" value="DUF977"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06163">
        <property type="entry name" value="DUF977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="105" mass="12090" checksum="34C6F555B1975A61" modified="2002-06-01" version="1">MLRSLREALETLRRHVQVLEVVAEHQPIGIGRISDVTGMEWHKVRYSLRVLEEGGIIRPSKKGAVLTEDAPARISKISRRLSELNREFEDISKRYQSLARKLEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVI5</accession>
    <name>Q8TVI5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation elongation factor, GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1404"/>
    <dbReference type="EnsemblBacteria" id="AAM02617">
        <property type="protein sequence ID" value="AAM02617"/>
        <property type="gene ID" value="MK1404"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1404"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1559"/>
    <dbReference type="HOGENOM" id="CLU_007265_0_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="9"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="358" mass="38158" checksum="B9BC74809BF28ACA" modified="2002-06-01" version="1">MERLEGGGVSVVNVVVCGHFNHGKTTLVKALTGEWLDRLPHEREMGVTIEPARAFLELGDTTVSFVDVPGHRDYIRNMLASAWSADYAILVVAADEGPCPGTIDHALVVSFYGARVLPVVSKVDLVSRDRAAEVADEVMDLLELLGVEAVVEPVPVSAKTGEGAEELLDALAELEEPPRPSEMDPLRAPVESTRRVDETTVDVFGIVDRGTLEEGRVEVQPGGRSAEVLEVESTDGSGPGVPFRARLRVKGSITRGMCLGEGLSTADRIEAEVLSIGAKVRPGTMGKVHVLSAAANVRFAEVETDAGTEVLRPYAEGHNVAHVVLELDREVVVEPGDRFLVTVGNEPAVLGVVEGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL1</accession>
    <name>Q8TVL1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1378"/>
    <dbReference type="EnsemblBacteria" id="AAM02591">
        <property type="protein sequence ID" value="AAM02591"/>
        <property type="gene ID" value="MK1378"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1378"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1533"/>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="1">
        <location>
            <begin position="10"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="2">
        <location>
            <begin position="264"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="394" mass="42063" checksum="A816CFFFFDE634E4" modified="2002-06-01" version="1">MVCVRFRRDVAIVGVGMTRFGELWERSFDDLFVEAGLEALEDAGMGGDEIEAMYVGNMSAGRFIDQEHVASLIADRSGLTPIPCTRVEAACASGGLAVRQAILAVASGMYDIVLAGGVEKMTDVTTEEATATLATAADQEWEAFHGVTFPALYAMIARRHMYEYGTTREHLALPPVKNHRNATKNPKAQFQFEITVEQVIESPLVADPLRLLDCSPVSDGAAAVIVCPLEMAKEFTDTPIVVRATAQASDSIALHDREDITTLKATVEAAKTVYKQAGVEPEEVDVAEVHDCFSIAELVAVEDLGFVEKGEAGEAYHEGMFEIDSDYVAVNPSGGLKAKGHPVGASGVAQVVEIVEQLRGEAGKRQVDGAEIGLTHNVGGSGGTVVVHIFERAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVL6</accession>
    <name>Q8TVL6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1373</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02586">
        <property type="protein sequence ID" value="AAM02586"/>
        <property type="gene ID" value="MK1373"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1373"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1527"/>
    <dbReference type="HOGENOM" id="CLU_896054_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="310" mass="35633" checksum="ECDE92701B01C432" modified="2002-06-01" version="1">MPVPVKNRATLAQLMLSYGITICVDEDVLRAINEMTFGQLPMILDESVNVPLEYEENIIDAELVSLLEFGEDMVSSLLDMIVVVRVNAVNGTGTPWELLNTILLADNPGKVELNGSYFSIFAIDERQIVYISSSGRRRIPYTDLTYMKDISEWVNARNIMSPNYAYIYDESALNITEDVLKKRYRASIKGLSELLDRPKHWVRIDTTITEKKDGILIRDRLLDWIEPLEFTKITVRRDVDEPAIEAITGESKVNKVYALRVFREIKPGFKTYRELVERAQLPDSIVKSTLALFTRFNFLMYSFKYTPPII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM4</accession>
    <name>SYL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">MK1364</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVM4"/>
    <dbReference type="SMR" id="Q8TVM4"/>
    <dbReference type="STRING" id="190192.MK1364"/>
    <dbReference type="EnsemblBacteria" id="AAM02577">
        <property type="protein sequence ID" value="AAM02577"/>
        <property type="gene ID" value="MK1364"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1364"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1516"/>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="OMA" id="AWNMAFQ"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="PTHR45794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152132" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="910"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="623"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="919"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="943" mass="108672" checksum="9B5D4567D2588094" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVM9</accession>
    <name>Q8TVM9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1359</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02572">
        <property type="protein sequence ID" value="AAM02572"/>
        <property type="gene ID" value="MK1359"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1359"/>
    <dbReference type="HOGENOM" id="CLU_2565766_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="81" mass="9296" checksum="DEC93C34C2EE14E4" modified="2002-06-01" version="1">MRRREPTYQVSLDGLRESTSALVIPPTRSRRVYALDRVLERPHSVLGERVGSYGQLEGRSSQSLTRIMETQNEIRQDPPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVQ8</accession>
    <name>Q8TVQ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02543">
        <property type="protein sequence ID" value="AAM02543"/>
        <property type="gene ID" value="MK1330"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1330"/>
    <dbReference type="HOGENOM" id="CLU_372858_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="745" mass="83202" checksum="888080A3CF15A291" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVR4</accession>
    <name>Q8TVR4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-31 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02537">
        <property type="protein sequence ID" value="AAM02537"/>
        <property type="gene ID" value="MK1324"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1324"/>
    <dbReference type="HOGENOM" id="CLU_1313133_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="209" mass="22301" checksum="1B1224FE07C7FA52" modified="2002-06-01" version="1">MFLTGVPLALWGLRLIAAGTGLPLPLVGALAWLFQWVFDENEEPDVVPPSYWVLPRVVLSKPPTWYLERALESPDTLLDYLLTGAVVVSLPLPLTQLVWVLLGGPTGVLEILDYLVQLIGEIMEDPGRTLPEVILWFLLPVLPHVVHVPTWLAALGGLPALLAHAGGLLLAPHFLTHAPAVTFGATVTVSRGGSCPTGVRNVSASGPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS7</accession>
    <name>Q8TVS7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Homolog of the eukaryotic argonaute protein, implicated in translation or RNA processing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02524">
        <property type="protein sequence ID" value="AAM02524"/>
        <property type="gene ID" value="MK1311"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1311"/>
    <dbReference type="HOGENOM" id="CLU_388137_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="2">
        <location>
            <begin position="557"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="628"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="711" mass="78687" checksum="1603C4EE6A460BE4" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-06-13" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS8</accession>
    <name>CAS2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas2</name>
        <name type="ordered locus">MK1310</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVS8"/>
    <dbReference type="SMR" id="Q8TVS8"/>
    <dbReference type="EnsemblBacteria" id="AAM02523">
        <property type="protein sequence ID" value="AAM02523"/>
        <property type="gene ID" value="MK1310"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1310"/>
    <dbReference type="HOGENOM" id="CLU_2285052_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000417744" description="CRISPR-associated endoribonuclease Cas2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01471"/>
        </source>
    </evidence>
    <sequence length="101" mass="11614" checksum="CB73F3E136B32E58" modified="2002-06-01" version="1">MGVLGGPSPRLRLYVYDFKEPGGEAERRKLRELLESHGAFRLQYSTYALLAEPEVHARVLRRVVARVDFEEGDSLIVVPMCRRCLRVARWVDAEGVRGLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVS9</accession>
    <name>Q8TVS9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02522">
        <property type="protein sequence ID" value="AAM02522"/>
        <property type="gene ID" value="MK1309"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1309"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="207" mass="24345" checksum="69BAB9EE21D63B10" modified="2002-06-01" version="1">MVESHAHVPIPSDPVERIRALRVLREVYRRGKKPGFEVTYRTVSGSTCGPYYVARWRRDSKFRHGRTLYLGKPENESVRFTEWLVSLDRSEVLELARHLMRNLRSVLKTLLTEVSSLPYKRARCVLTRGLALAFDARPSNSPRIRDLLEELPDRLESFLVRTLGGWPAHYSSHLNKIIRSRRKSLDGRHEVPDVQLELERWRLRHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU1</accession>
    <name>Q8TVU1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted DNA-dependent DNA polymerase, component of a thermophile-specific DNA repair system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02510">
        <property type="protein sequence ID" value="AAM02510"/>
        <property type="gene ID" value="MK1297"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1297"/>
    <dbReference type="HOGENOM" id="CLU_353997_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038242">
        <property type="entry name" value="Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024615">
        <property type="entry name" value="CRISPR-assoc_Cmr2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013407">
        <property type="entry name" value="CRISPR-assoc_prot_Cmr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12469">
        <property type="entry name" value="DUF3692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02577">
        <property type="entry name" value="cas_TM1794_Cmr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="541"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="793" mass="88611" checksum="1954F3E9975A8F63" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW2</accession>
    <name>Q8TVW2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fusion of at least two uncharacterized domain specific for M.kandleri, MK-12 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1276</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02489">
        <property type="protein sequence ID" value="AAM02489"/>
        <property type="gene ID" value="MK1276"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1276"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1392"/>
    <dbReference type="HOGENOM" id="CLU_607818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="238"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="450" mass="49459" checksum="9E857B9B3D1FC63C" modified="2002-06-01" version="1">MVATVRPVRALPGSSTWVRLSSECIVVRGPDGTVRVRPEDVVSVKEHRSSRPRMDPDEEVRLVLITRDLEHVVLPFRSRKLYRGVKAILAARDGDVDRLLRHLRRVLRKERKPPLFERREDGVWLRWPWLEVRVGPPERARRVVRELLEYWLRLLKESGRDPEDVSPRKVGLSVVLGSLSILFGGGKGKKGAAGAKVKAGAKGDAGGKTGGTALAKASKKLLLKMIKALMKLGVKVTFWTLVAFVVAVALVPLAVMSVVYWLIARRAYRWGSVPWLRGLVLLQLQGFAVLTGFWCLFAPLPVLFGHFLAGPFVVAGAAALVPPSLLLVRAYRREPLRFPVAVHTTGSDVTRPLTLAGVGRAGGGAVRGARLGRPVRRGGRRPLAGARLRRTGPDDPARVEAGPAPVAAPGGRPDRMNGLDRSVGWGTWTEPVTQLARRGDRYFFSPFDSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-05" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW09</accession>
    <name>SYP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proline--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="1">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proS</name>
        <name type="ordered locus">MK1229</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TW09"/>
    <dbReference type="SMR" id="Q8TW09"/>
    <dbReference type="STRING" id="190192.MK1229"/>
    <dbReference type="EnsemblBacteria" id="AAM02442">
        <property type="protein sequence ID" value="AAM02442"/>
        <property type="gene ID" value="MK1229"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1229"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1332"/>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PTHR43382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="SSF64586"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000249159" description="Proline--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <sequence length="485" mass="56218" checksum="E540F47E5F81DB0A" modified="2002-06-01" version="1">MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVEKLRRKLRETGHEEVLFPTLIPETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTSETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLIRMREITTFKEAHTAHATEEEAEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHMLGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVVMVPILKKGVRRKILERAAEVEEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGARELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAMELRKRAREKFEKGIFRTDSPEEARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVRIAKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW57</accession>
    <name>Q8TW57_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02392">
        <property type="protein sequence ID" value="AAM02392"/>
        <property type="gene ID" value="MK1179"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1179"/>
    <dbReference type="HOGENOM" id="CLU_1773198_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="146" mass="16840" checksum="5EAFDFEB394F7620" modified="2002-06-01" version="1">MEIEVTEGSFVSEVYHWLRKEGLPAVAQVRIPGVRGRVDLLVMDRRPVVVEVGFPGVEITDEDLRRALRYVRAIIEWNSDVEPAAIVTNLKEAWYRDDPLGEWEKEELEDPTSDLIVLVRRLARAEIETEEQKGPEETEDTDRMLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW62</accession>
    <name>Q8TW62_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02387">
        <property type="protein sequence ID" value="AAM02387"/>
        <property type="gene ID" value="MK1174"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1174"/>
    <dbReference type="HOGENOM" id="CLU_1302634_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="211" mass="23868" checksum="2BB25D2C82A18690" modified="2002-06-01" version="1">MELHERHLAPSTTGPVTLYGVPIPVLIAMPFYLAAVGRGVLALKWAWRAGNTVRTASIVASFAVLTLLYLSVPTVTVAAITLLVSTVLFYLFNTFIPRYDRFYVEYRRRWGYDEAPKHEVLELARECRLDVLPPALKLLNGTAYLAAYNERDNPNGWDPIDRFHRLLLAFEEGSWKGGYVGPNAWRIVRGVAELSLDHPEVELARRLEEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW90</accession>
    <name>Q8TW90_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein specific for M.kandleri, MK-9 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1146</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02359">
        <property type="protein sequence ID" value="AAM02359"/>
        <property type="gene ID" value="MK1146"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1146"/>
    <dbReference type="HOGENOM" id="CLU_926260_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="300" mass="33875" checksum="2B71666DFC28BDC5" modified="2002-06-01" version="1">MMLCLTVSSALPAALTLGFIGFPRMRGDRRRLSIALLSLGYCEAELITDGEVEVSRFRELSSYPVRYGEAPGGQPSNLRIRVGYFRPGPCKVEVRDCGFLFLRTRGVCVLHDAQGLLKDREKAFHLLRWWEKFSMVYAALCLEKEESPEPEEVDGLLGSVVNNRAGWNELVYLNRRFSLTDVLRHYEVAVSMLGTGVCCLVTPAWVKLIGVKPSLKLLHTWWYLAPAILLIPPVSALGVFVINLDTALRSWRLVREGRVYESVLGRDPYDGKWYGKWLSRDGDPIASRHRESESDEPQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW93</accession>
    <name>Q8TW93_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02356">
        <property type="protein sequence ID" value="AAM02356"/>
        <property type="gene ID" value="MK1143"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1143"/>
    <dbReference type="HOGENOM" id="CLU_914019_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="34093" checksum="24D5506A68B06DF5" modified="2002-06-01" version="1">MSTDDGWIVVRVSSFKYHRVGIVTFGGHVEVDAWRVHTFLRDEELAERLADVLPGRGELLACEGLLSSYHEVFVRPRGDEIRIKPSPGAHTIVLVSRGFVLTRKPERVRELRARAVLAVAGLMEGKVSVPKRWIKAFVLGSRGRLAGIEVKSLAYLGFEAIDGIPPIVGPSTSGLIVLSIDAWDEVCEKLGVGWELEVDIKTVLLILTISYFVAVGACIVRTLRGGREPKELHYARMWEELEGDSNGVGEATIADTRKEEDSEEDVEYDEPGKWIRKHRRFKSLPDYYEIAGIRRRNGERSRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA3</accession>
    <name>Q8TWA3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02346">
        <property type="protein sequence ID" value="AAM02346"/>
        <property type="gene ID" value="MK1133"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1133"/>
    <dbReference type="HOGENOM" id="CLU_2645919_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="76" mass="8438" checksum="9B8627F58BDBA579" modified="2002-06-01" version="1">MDTSGHRWVLVKVKYFPAWHARGGRILMGPGGTMIVRTGSGRMILYFAYPIRLHALGALALALQWINAPVRAMMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWC9</accession>
    <name>Q8TWC9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitrogen regulatory protein PII homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02319">
        <property type="protein sequence ID" value="AAM02319"/>
        <property type="gene ID" value="MK1106"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1106"/>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="KYTIIEI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024184">
        <property type="entry name" value="UCP005637"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005637">
        <property type="entry name" value="UCP005637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="108" mass="12117" checksum="0678F55B81C69214" modified="2002-06-01" version="1">MKEVRLFVDPENVGRVMNAMADVGVTGFYAIEYRGVAPDRWAGFEIREDPESAIKALNDLSERAVMIVTVVPEECVEKLKDAAAERLAGERYTIIVTDVEEIHVDYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE4</accession>
    <name>Q8TWE4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-methylhydantoinase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hyuA_1</name>
        <name evidence="3" type="ordered locus">MK1091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1091"/>
    <dbReference type="EnsemblBacteria" id="AAM02304">
        <property type="protein sequence ID" value="AAM02304"/>
        <property type="gene ID" value="MK1091"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1091"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1146"/>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="OMA" id="HAMTVSV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydant_A_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase_A" evidence="1">
        <location>
            <begin position="198"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="680" mass="74685" checksum="146A31ABEE930B39" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-25" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG3</accession>
    <name>PUR2_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purD</name>
        <name type="ordered locus">MK1071</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG3"/>
    <dbReference type="SMR" id="Q8TWG3"/>
    <dbReference type="STRING" id="190192.MK1071"/>
    <dbReference type="PRIDE" id="Q8TWG3"/>
    <dbReference type="EnsemblBacteria" id="AAM02284">
        <property type="protein sequence ID" value="AAM02284"/>
        <property type="gene ID" value="MK1071"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1071"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1125"/>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151511" description="Phosphoribosylamine--glycine ligase">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="112"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="139"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <sequence length="449" mass="49275" checksum="FFD9F9189C9ADC48" modified="2002-06-01" version="1">MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVARAAADWNVDFAVVGPEDPLAEGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDIPGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVKVVGDQLKNLDEAKEYVKEIFEEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYSCPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRYGDPEAMNILPIAEGDIVTLHASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVIEVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGIGDELEQAEEAAESAIRECVSGERIRHRSDIGKHETVEKRVRRMKRIRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH9</accession>
    <name>Q8TWH9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1055"/>
    <dbReference type="EnsemblBacteria" id="AAM02268">
        <property type="protein sequence ID" value="AAM02268"/>
        <property type="gene ID" value="MK1055"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1055"/>
    <dbReference type="HOGENOM" id="CLU_007727_8_0_2"/>
    <dbReference type="OMA" id="GRAMGFC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="2">
        <location>
            <begin position="18"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="352" mass="39012" checksum="3AF6C5E7F3145FB9" modified="2002-06-01" version="1">MALVYHEEYLRHEQHPTHPERRERLSYTVDRFEEEGLFEIEGIDLVEPDPVDREVIELVHDPEHVELIRRMSESGGGMIDLDTAVAPETYDQALLAAGGSVLAVELVVRGEYDTAFAMVRPPGHHAGRAKAAGFCYFNNAAIAAEYAIRELGVDSVAILDWDAHHGDGTQEIFYDRDDVLYVSIHQDGRTLYPGTGFPYEAGEGPGEGYTVNIPVLPRSGDRTYREAFQRIVEPVVREFDPDLILISAGQDCHFTDPITDLAVTAEGYRWMMQRATELAEDLGASGPVAVLEGGYSVEDGLPYTNLGVVCGMAGVPADLREPMDPQEENPRGPEGVRKVAREHSRYWTSVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI5</accession>
    <name>Q8TWI5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1049"/>
    <dbReference type="EnsemblBacteria" id="AAM02262">
        <property type="protein sequence ID" value="AAM02262"/>
        <property type="gene ID" value="MK1049"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1049"/>
    <dbReference type="HOGENOM" id="CLU_166059_1_0_2"/>
    <dbReference type="OMA" id="YPGVCPY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011411">
        <property type="entry name" value="MazG-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004518">
        <property type="entry name" value="MazG_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03819">
        <property type="entry name" value="MazG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036521">
        <property type="entry name" value="UCP036521_pph"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MazG" evidence="1">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="95" mass="10998" checksum="96FB7CB01D8ECE63" modified="2002-06-01" version="1">MEISEFQRLMDELYGEKDRKRGPERTLLWLVEEVGELAEAVRKNDRQAVREELADVVAWTFSLANVLGIDVEEVLKEKYPGRCPKCGEIPCRCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL4</accession>
    <name>Q8TWL4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02232">
        <property type="protein sequence ID" value="AAM02232"/>
        <property type="gene ID" value="MK1019"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1019"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1070"/>
    <dbReference type="HOGENOM" id="CLU_137479_3_1_2"/>
    <dbReference type="OMA" id="MIVAEFS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="SSF89957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00106">
        <property type="entry name" value="TIGR00106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine_BP" evidence="2">
        <location>
            <begin position="5"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="101" mass="11148" checksum="BCEABA4E8D020134" modified="2002-06-01" version="1">MAVVMEISFYPIGTGSPSVSDEIVEVVKALKEAGFEPQVGPMGTVVEVETFEDALEALRVAREAALRVVDRAVFVVKIDERRDKELTAEGKVRSVERKIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWL8</accession>
    <name>Q8TWL8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Archaeal/vacuolar-type H+-ATPase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ntpC</name>
        <name evidence="5" type="ordered locus">MK1015</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1015"/>
    <dbReference type="EnsemblBacteria" id="AAM02228">
        <property type="protein sequence ID" value="AAM02228"/>
        <property type="gene ID" value="MK1015"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1015"/>
    <dbReference type="HOGENOM" id="CLU_738914_0_0_2"/>
    <dbReference type="OMA" id="LRKFDVW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_su_c/d_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_su_c/d_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_suC/d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="SSF103486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="374" mass="41734" checksum="FFDCD509666DA10A" modified="2002-06-01" version="1">MLGLAGLQDYTLLAVGIIALVTVLSIILSIPITDVVWRYAPYAYPLPRAKAVEAETLSDEDYEELKDAPVRDLVTRLEELGVEPEAARAVLDGNTAPLEIELKRRAVESVMERIVETSPEDVSELARALIAKYELEDIKAVLRARHAGEEPKHLVDAPVLLGAETWRELKEARSVPEVVDYLRGTPYDRGLEEALREYEETGSLLPLELALDRAYYEHLWDLVVSEKVDEELARLVGLEIDLYNVEVALRGAILNLDPERVLEAMAEGGWELAEWRKRELAEAEDPLEVVERLSGTSLGPYLEEAAEEYSEGRGVQVFDEALRKARYELAREIAGSDLIGAPAVVHAVYEKQREVDNVISLVNAKVADVETMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWN1</accession>
    <name>Q8TWN1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">MK1001</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1001"/>
    <dbReference type="EnsemblBacteria" id="AAM02214">
        <property type="protein sequence ID" value="AAM02214"/>
        <property type="gene ID" value="MK1001"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1001"/>
    <dbReference type="HOGENOM" id="CLU_097041_0_0_2"/>
    <dbReference type="OMA" id="KEDCIRC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001080">
        <property type="entry name" value="3Fe4S_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00352">
        <property type="entry name" value="3FE4SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="98"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="299" mass="32904" checksum="E93FA6FA1FF17AD1" modified="2002-06-01" version="1">MPIEIDMDSCLLCEACVAACPTGAIRREDGDMNHCIVCGACVKACPVDALELEDLEREVDGEKVELKRIAWKPEECHKEDPPQCGEVCPQEIMRIEDGYPELRGFCVMCLKCMETCPIDAIGMKGVVEPKSEPPEHPEDEDVYVHPERCVGCTYCLQVCPTDAIEMVPTDAEFFKAEENEWSPVYVDPDTMELKQGRKVAKIDPEKCTGCTLCAQVCPWGAITAARDVPVQSREVKNEIDEDKCVGCGVCAEVCPGDLIEVDGVAKVPEKCPACKLCERACPVDAISINVSYERSGEME</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-01-17" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP6</accession>
    <name>SYK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="1">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysS</name>
        <name type="ordered locus">MK0986</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWP6"/>
    <dbReference type="SMR" id="Q8TWP6"/>
    <dbReference type="STRING" id="190192.MK0986"/>
    <dbReference type="EnsemblBacteria" id="AAM02199">
        <property type="protein sequence ID" value="AAM02199"/>
        <property type="gene ID" value="MK0986"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0986"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1033"/>
    <dbReference type="HOGENOM" id="CLU_025562_1_0_2"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152754" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="37"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="301"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <sequence length="555" mass="63462" checksum="A06B1DF2DB4B8E80" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWR9</accession>
    <name>Y963_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MEMO1 family protein MK0963</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0963</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWR9"/>
    <dbReference type="SMR" id="Q8TWR9"/>
    <dbReference type="STRING" id="190192.MK0963"/>
    <dbReference type="EnsemblBacteria" id="AAM02176">
        <property type="protein sequence ID" value="AAM02176"/>
        <property type="gene ID" value="MK0963"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0963"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1008"/>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PTHR11060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134380" description="MEMO1 family protein MK0963">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <sequence length="283" mass="30563" checksum="8FAC6C3167F75633" modified="2002-06-01" version="1">MERSPAVAGQFYPADPEELRKMIEWCFRHELGPGDLPETNDGPCTLPGVVAPHAGYQFSGPVAAHTYKVLAESGTPETVVILGPNHTGLGSAVATMTDGAWRTPLGSVEIDSEFATALVRKCGVMDDDLTAHANEHSIEVQLPFLQYVYGESFRFVPVCMAMHDLQTAREVGEAIVDVAEELDRNTVVIASTDFTHYEPHDQAQKKDRKVIERITALDEAGMIEIVERYNVSMCGVGPTAATIVAVKAMGASEGELLKYATSGDVSGDYSQVVGYAAIVFRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWS1</accession>
    <name>Q8TWS1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Spermidine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">speE</name>
        <name evidence="1" type="ordered locus">MK0961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0961"/>
    <dbReference type="EnsemblBacteria" id="AAM02174">
        <property type="protein sequence ID" value="AAM02174"/>
        <property type="gene ID" value="MK0961"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0961"/>
    <dbReference type="HOGENOM" id="CLU_1122661_0_0_2"/>
    <dbReference type="OMA" id="RTWRTRI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="232" mass="26204" checksum="CBFBF727B1B07155" modified="2002-06-01" version="1">MGVGEVVDNIGRGLLSEDRRAREAAARYREWAESLDEEELFRYVRERTGGLGHGKEGFEVGKMGLKGYVIAWPEQARGMVLEIGTGLGRTTWALLRWGDPELIVSVEVDPRMLAIALYRNPVPEFSEALRDDRVKILLGDAVEVVPKLPRGFDHVVHDGGPCPGRNPRLFSPEFLRTIAEKLKDEGTASVFAGRDPRWQDRIYRALSRLFLEVRAESFPDTPTVVFRCEGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWW9</accession>
    <name>Q8TWW9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0912</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02125">
        <property type="protein sequence ID" value="AAM02125"/>
        <property type="gene ID" value="MK0912"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0912"/>
    <dbReference type="HOGENOM" id="CLU_1080145_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="28720" checksum="1C585839F91DD8F8" modified="2002-06-01" version="1">MSSDPRRPTKVPWVKAMVIGLIAVMSAKALFHEWVVATLHPTNKDRLVMKVERVPCRTGDDLRPGNVEVYAKVYGCTKYATLGYGSQDPRRILVPALPPRLMSLTVTVAGNVIRVFRTCFPAPFIIVTATKLPRISVNQYEIRIHSPCKILLQPTVTGLLKYFPPVFITDGKSTRNPTHLTCRNVAIIMPYALTVMGGLTPIDVVSVVSVSGERLVVNASSVYAPKGVTIKWEPICPLIWTTYADIVWRWPYWPYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX01</accession>
    <name>Q8TX01_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyltransferase involved in cell wall biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0880"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02093">
        <property type="protein sequence ID" value="AAM02093"/>
        <property type="gene ID" value="MK0880"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0880"/>
    <dbReference type="HOGENOM" id="CLU_033536_13_1_2"/>
    <dbReference type="OMA" id="TAYIHGF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="PTHR43398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="1">
        <location>
            <begin position="5"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="240" mass="27559" checksum="5978FF37BDB050A2" modified="2002-06-01" version="1">MVDVSVILPTYNERENLPRVIPKIEEVVEEEGWTAEILVVDDNSPDGTAEVARELSRQYGNIKVIVREEKPGLGLAYRRGFREARGEVIVCMDADGQHPPECLPNIVNPVLDGECDFGLGSRYVEGSVVENFPWYRKLNSWGARVVARLFLKLPYRDPTSGFRAISRKILTESRPFVSEGFEIQVETLAKAHHMGYTVQEYPFLFRPRERGSSNVNIRQILRYLRGVWRIRKDLKQRGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX09</accession>
    <name>Q8TX09_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Chloride channel protein EriC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">eriC</name>
        <name evidence="6" type="ordered locus">MK0872</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02085">
        <property type="protein sequence ID" value="AAM02085"/>
        <property type="gene ID" value="MK0872"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0872"/>
    <dbReference type="HOGENOM" id="CLU_726865_0_0_2"/>
    <dbReference type="OMA" id="FTRVGHD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="380" mass="39653" checksum="0908750A02AD3869" modified="2002-06-01" version="1">MPSTGALIHVARWTVLAAALGVVGGACAVAISSIISTVEKAVGNEPLVIPAFMLLAGSLAALHPELRGTGMEIIVRGFPNEPANPIRGMAKLVLAGLVIGGGGSGGQVGPCAQACASVGGYVASKLGMSEMERKWAVLGVISGGVAGVLCAPLAAALFAIEVLRVRSRYVVLFPSTIASLAGYSVYVSTLGKRYLLVRNAPYHYSPQHLPELLVIAVVATLLAYVYSRCVKEARRLFVESVPSQPVRSLLAGLGVASVGIAIPTAVGLGLDYASKAALGRIGAEEALLSFLGKMVATALTVGSETPAGLVSPTVCVGAFLGLFWVKRWARPLRPEPRRLSLRSWLRRSTLRSRPLCWSSSCSASTAPSRPLWERSWGISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX12</accession>
    <name>Q8TX12_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0869"/>
    <dbReference type="EnsemblBacteria" id="AAM02082">
        <property type="protein sequence ID" value="AAM02082"/>
        <property type="gene ID" value="MK0869"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0869"/>
    <dbReference type="HOGENOM" id="CLU_040681_9_2_2"/>
    <dbReference type="OMA" id="EDVVFAF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="16"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="81"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="138" mass="15414" checksum="9BCF6515B4258E9B" modified="2002-06-01" version="1">MTFREKLRGCKVEELMTKDPITASPQVGVIEAFEIMLKHDVGALPVVDDEGRLIGLVTRTDLGRALLEDEYEPGTTVEEVMERDVVVVHPDDTLLEALKRMTSAPEGIYNQLPVVDDEEKLVGILTDGDILRWIAKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX49</accession>
    <name>NTPTH_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside-triphosphatase THEP1</fullName>
            <shortName evidence="1">NTPase THEP1</shortName>
            <ecNumber evidence="1">3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK0827</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.15" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX49"/>
    <dbReference type="SMR" id="Q8TX49"/>
    <dbReference type="STRING" id="190192.MK0827"/>
    <dbReference type="EnsemblBacteria" id="AAM02040">
        <property type="protein sequence ID" value="AAM02040"/>
        <property type="gene ID" value="MK0827"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0827"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.869"/>
    <dbReference type="HOGENOM" id="CLU_103145_1_1_2"/>
    <dbReference type="OMA" id="GAMEFKC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="PTHR43146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146697" description="Nucleoside-triphosphatase THEP1">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00796"/>
        </source>
    </evidence>
    <sequence length="180" mass="20326" checksum="E41855768894B701" modified="2002-06-01" version="1">MPHNVVLTGRPGIGKTTVCLKVRNVLEEEGYTVGGIYCPEIREGGRRIGFEIVDLTEGDRYLLAREGASGPRVGRYGVFVDNLERAAESIERAVKRTDVVIVDEVGPMELKSNAFVDAVRRAADAHTPAIFVVHERSRHPVVVDLREERPDVVRFRVTLSNRDELSDRILEHVLEWLEER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX52</accession>
    <name>RL11_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl11</name>
        <name type="ordered locus">MK0824</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX52"/>
    <dbReference type="SMR" id="Q8TX52"/>
    <dbReference type="STRING" id="190192.MK0824"/>
    <dbReference type="EnsemblBacteria" id="AAM02037">
        <property type="protein sequence ID" value="AAM02037"/>
        <property type="gene ID" value="MK0824"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0824"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.866"/>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104436" description="50S ribosomal protein L11">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <sequence length="166" mass="18307" checksum="A75885C78B1F3B61" modified="2002-06-01" version="1">MPKEEVEVLIEGGKADPGPPLGPALGPLGVNIQEVVEEINRKTKDFKGMEVPVKIIVDTETREFEVKVGSPPTSAIIKSELGIDKGAHEPRHETVGDLSMEQVIKIAKMKFDDLLSYDLKTAAKEILGTCGSMGVTVEGKDPKEVQKEIDEGKWDDLFEKYEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX69</accession>
    <name>Q8TX69_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Possible Zn-dependent metallohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02020">
        <property type="protein sequence ID" value="AAM02020"/>
        <property type="gene ID" value="MK0806"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0806"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.847"/>
    <dbReference type="HOGENOM" id="CLU_762099_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="363" mass="40047" checksum="4551024B5F744E6A" modified="2002-06-01" version="1">MQRITIVALILSIAAVPGSADLMNPVYLTGTLAHELGHALVEAEYYGGIDEVQLDVFPPKILFHCRDGKCIDIHAEPPIGFVTRGPYDPGTPEEELRKRHKEAMLLYIEDPARFFDNVSREDAEVTMAGPVVGALISAALSPLDPQGAFDSAWDNLLNLMPVEIHGALTDGKIAWEALIRGRYPVERYLIELGLVTLSVLVTAAQCPRYALETIESDFVLTLELAPIALLDDLSKAVLGNLLGLAVKLREPFFVPGIWVGWNLSLLGPDHRKWLFALLDALGWTLVIPWLDMPWKLYAIILKLIDATADLDPGYPSLGSYLGRLKVPFIVFLLVSLLQCHPFLEDSKGIIIMEGSRLRRNGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX76</accession>
    <name>Q8TX76_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420-reducing hydrogenase, beta subunit fused to oxidoreductase related to nitrite reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0799</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02013">
        <property type="protein sequence ID" value="AAM02013"/>
        <property type="gene ID" value="MK0799"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0799"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.840"/>
    <dbReference type="HOGENOM" id="CLU_428041_0_0_2"/>
    <dbReference type="OMA" id="GDECWKP"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="531"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="565"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM02013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="668" mass="72335" checksum="EFF8F77EDD537616" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX85</accession>
    <name>PURQ_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">MK0790</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX85"/>
    <dbReference type="SMR" id="Q8TX85"/>
    <dbReference type="STRING" id="190192.MK0790"/>
    <dbReference type="EnsemblBacteria" id="AAM02004">
        <property type="protein sequence ID" value="AAM02004"/>
        <property type="gene ID" value="MK0790"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0790"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.831"/>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100610" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="226" mass="25032" checksum="8624A73899B59DF6" modified="2002-06-01" version="1">MVRVAVIRFPGTNCDLEMAWAVKLAGGDPEFVWHEDGGLDDFDAVIIPGGFTYGDYIRAGAIAALSPILEEIRECAEDGRPVLGVCNGMQILAEAELIPGTLTVNVGNRFICDWVYLRVERTDTPFTTKYQEDEVIRVPIAHAEGRYYYENPEEIEDNVVFRFCGPDGDVSEEYNLNGSVGGITGVVNDDGNVLGMMPHPERAAHRLLNSDDGLRLFESLVEWCRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXC6</accession>
    <name>Y748_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein MK0748</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0748</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXC6"/>
    <dbReference type="STRING" id="190192.MK0748"/>
    <dbReference type="EnsemblBacteria" id="AAM01962">
        <property type="protein sequence ID" value="AAM01962"/>
        <property type="gene ID" value="MK0748"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0748"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.788"/>
    <dbReference type="HOGENOM" id="CLU_138334_0_0_2"/>
    <dbReference type="OMA" id="KVFNAQS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="PTHR42199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016934">
        <property type="entry name" value="UCP016934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000068277" description="UPF0212 protein MK0748">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <sequence length="118" mass="12990" checksum="2BA641A069C616B7" modified="2002-06-01" version="1">MGEETGGDYHVVLEAAWIVYNVDDEDDAINIAIAEAGKRLNRHGLDYVDIDVGFYECPECGNEIEGVWIVAGTALVRLILSMRVFNAESEEHAIRIAKYEIGQALEDVPLGVVEVTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXD8</accession>
    <name>Q8TXD8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01950">
        <property type="protein sequence ID" value="AAM01950"/>
        <property type="gene ID" value="MK0736"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0736"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.776"/>
    <dbReference type="HOGENOM" id="CLU_1607168_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="165" mass="16895" checksum="921AEBAB2F2839A6" modified="2002-06-01" version="1">MGPELAAVSGFALKIADDLVDELSRPQWALPCGLVAAAAALASVATGVRPDLYLGLILGNAVAGKIDEIPHLAAAAIVALGVLITRPEVTPLTLLVITVLATLDEIIHPVEPTGLRPVLKIGAVVGWAFGVLDGITALAVITFDLGYHAAELLTGAIRCSNPIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF8</accession>
    <name>Q8TXF8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-40 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01930">
        <property type="protein sequence ID" value="AAM01930"/>
        <property type="gene ID" value="MK0716"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0716"/>
    <dbReference type="HOGENOM" id="CLU_346359_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="747"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="794"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="815" mass="89046" checksum="7B45CB7AFA317F0B" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-13" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG4</accession>
    <name>SYDND_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">MK0710</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXG4"/>
    <dbReference type="SMR" id="Q8TXG4"/>
    <dbReference type="STRING" id="190192.MK0710"/>
    <dbReference type="EnsemblBacteria" id="AAM01924">
        <property type="protein sequence ID" value="AAM01924"/>
        <property type="gene ID" value="MK0710"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0710"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.751"/>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="PTHR43450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000110996" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="222"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <sequence length="431" mass="49791" checksum="8AFBE2175AE8AF74" modified="2002-06-01" version="1">MLKDAYTADVTPERDGEEVRLAGWVHEVRDLGGIKFVLLRDRTGIVQLTLPKQKVPKETFEKVPKLTKESVIRVEGTVQANEKAPGGVEVIPQRIEVLSESDTHLPLDPTGKVDADLDTRLDARVLDLRREEPQAIFKIRNVVTTAIREFLEERGFIEVHTPKIIASATEGGTELFPVVYFERDAYLAQSPQLYKQMLMAAGFERVYEIGPIFRAEEHNTRRHLNEAISVDIEMSFIESEEDVMRVLEELLAHVFRKVREECEKELEALDRELPELETPFERITYEETLDLLSEHGIEVEWGEDLPTEAERKLGEIFEEPFFITEWPRETRPFYTMAKDDEVTTAFDLMYQGLELASGAQREHRYDVLVRQIEEQGLSPEDFRHYLEAFKYGMPPHGGWGLGLERTLMTITGAENIREVTLFPRDRKRLHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH0</accession>
    <name>Q8TXH0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01918">
        <property type="protein sequence ID" value="AAM01918"/>
        <property type="gene ID" value="MK0704"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0704"/>
    <dbReference type="HOGENOM" id="CLU_835784_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="332" mass="37842" checksum="C28E3710AE093707" modified="2002-06-01" version="1">MKLVITVDLFGERGHHRLGALPRDAHLTVFTDYLSILEMRDALEAVDWDIHCTTYSDVPLTYVPEEDAQAHIELSSEFGECFRAPALAVNKALLRKLPELGFDADSSGWSRKGRVGRLYEVTEAPIVCDVMDRKFLNKVLSRLDSYDVVVLRSGSEIPGNRHFKIVDKIASSLSVAPLSEVLEEADGRVRAKFNDRGILTEREHLLLTALSEVWRLHSELSEEVGDRELILAHLASESFRNPQRVLTVCKRFAEELGSEYTERLARVSKRPIVIGALRKLSRRFQIEMEEEDERLRVQELKRRVEAIDRGIRMLAQDRPIEGWNEILSVLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-04-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH1</accession>
    <name>Q8TXH1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prepilin-cleaving (Type IV) signal peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pppA</name>
        <name evidence="4" type="ordered locus">MK0703</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01917">
        <property type="protein sequence ID" value="AAM01917"/>
        <property type="gene ID" value="MK0703"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0703"/>
    <dbReference type="HOGENOM" id="CLU_716919_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_A24" evidence="2">
        <location>
            <begin position="8"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Arc_PepC" evidence="3">
        <location>
            <begin position="224"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="324" mass="34427" checksum="A3F546241197CB39" modified="2002-06-01" version="1">MDVGFLAGLAVATVAAITDLKWGIVPNRLTYPAIVAGAIYAAVIDPSHLGYALLDAGVAFGVTLVLNVLGVLGGGDVKLLPSLTLFLHRGDRITTGIDVLFNSILLYAPFALLYLTVSTALDRGRPFLKELCLNTTILVLASLIAGGVATILGTVISSVVGILLILAVWKVARSYESKLKIVLPALVVPAVLLNLRASPIAVLWEVGIAVALSTAFTTYRWAGKERNVEELREGEIPLEIVVRTEEGTERVGRLKGALLVATGRAEPVVVPSGDGFTEEELEKLKRLGIDRIRVGHTTPFAPAIAAGYVVTYMLQGSPLSWLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXI6</accession>
    <name>Q8TXI6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpoB_2</name>
        <name evidence="11" type="ordered locus">MK0687</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0687"/>
    <dbReference type="EnsemblBacteria" id="AAM01902">
        <property type="protein sequence ID" value="AAM01902"/>
        <property type="gene ID" value="MK0687"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0687"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.726"/>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_2"/>
    <dbReference type="OMA" id="GKICPSE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA_pol_Rpb2_1" evidence="9">
        <location>
            <begin position="29"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_2" evidence="8">
        <location>
            <begin position="205"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_Rpb2_3" evidence="10">
        <location>
            <begin position="435"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AAM01902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="525" mass="61121" checksum="A2C949D85420399F" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ1</accession>
    <name>Q8TXJ1_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nusA_1</name>
        <name evidence="5" type="synonym">nusA</name>
        <name evidence="7" type="ordered locus">MK0682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0682"/>
    <dbReference type="EnsemblBacteria" id="AAM01897">
        <property type="protein sequence ID" value="AAM01897"/>
        <property type="gene ID" value="MK0682"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0682"/>
    <dbReference type="HOGENOM" id="CLU_131906_0_0_2"/>
    <dbReference type="OMA" id="TDQIKYI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="KH" evidence="6">
        <location>
            <begin position="32"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM01897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="142" mass="15909" checksum="BF4FEFC4E03B2677" modified="2002-06-01" version="1">MAVKLETEQIRMIALFESLTGAHVLDCVIDDEHNRAIFVVKEGQIGLAIGKKGQNVRRVQEQLGMDVEVVEHSEDPEKFIRNALFPARVKSVRVTERGNKKVAIVDVPESERGRVIGKGGRNIKKARILARRHHGIDDIIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ8</accession>
    <name>Q8TXJ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NCAIR mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0675</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0675"/>
    <dbReference type="EnsemblBacteria" id="AAM01890">
        <property type="protein sequence ID" value="AAM01890"/>
        <property type="gene ID" value="MK0675"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0675"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.714"/>
    <dbReference type="HOGENOM" id="CLU_065705_0_0_2"/>
    <dbReference type="OMA" id="EVIWGPG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PTHR43064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="1">
        <location>
            <begin position="88"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="24816" checksum="C24DB000791C1875" modified="2002-06-01" version="1">MKVVRPDRRRTRRTGFPEVVMAEGKTPEQVSEALEALLRSEGIAIASRVDPELLDDLEIPEDADVEYDEAGRVLVLKDPEYEPPYRGGRVGAVAAGTSDLPYLSECATVLEALGIEVHTEIDVGVAGPHRLLAAVRRLRKFRPHAVVAAAGMDGTMPVALNAMLDVPVIGLPTPVGYGLGGSGEAALLGMLQSCSPGLVVVNVANGIGAAAAAVKIVRVCLEGEKPSKKPRNSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL2</accession>
    <name>Q8TXL2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fe-S oxidoreductase, related to NifB/MoaA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0650</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0650"/>
    <dbReference type="EnsemblBacteria" id="AAM01865">
        <property type="protein sequence ID" value="AAM01865"/>
        <property type="gene ID" value="MK0650"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0650"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.689"/>
    <dbReference type="HOGENOM" id="CLU_668380_0_0_2"/>
    <dbReference type="OMA" id="IHLGYTS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017672">
        <property type="entry name" value="MA_4551-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03278">
        <property type="entry name" value="methan_mark_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="405" mass="45278" checksum="DACC5B81A5618F53" modified="2002-06-01" version="1">MGGRPGVDCGGFCRYCYFRGVDWEEKRPFGCKNCPPGSPGCDYCGRSVWEDNGPFRPPGVVLQEVGMSLGFRREREATVSGGGDISNYPWLRELVASLRDLGIRSVQLGYTSGKGFEDPEEVEELCDLGVRSVSYTVFAWDPELRREWMGDRSPEASLACLEVFADRCEEVMVAAVLIPGVNDGDVLWKTCERLEELGIDALLLMRLGTREEHGIILGNSLVLDVKPHSLDEFKRIVTEVHEEFPFRVTGTPLWDPETSAPFALRTLGEELRELLPPVEVECSLITGRVAAPLIREVFRHVEGGEKVDVVPVEKDVACLITERDLERLDVSSLKRTVVLPGRALVHDARAEELLKRDGFERVVLRGPDRLTLDGEASCEVDREEVLEFELNAFRDLINTVNILGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM7</accession>
    <name>Q8TXM7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation factor (SUA5)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sua5</name>
        <name evidence="2" type="ordered locus">MK0635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0635"/>
    <dbReference type="EnsemblBacteria" id="AAM01850">
        <property type="protein sequence ID" value="AAM01850"/>
        <property type="gene ID" value="MK0635"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0635"/>
    <dbReference type="HOGENOM" id="CLU_031397_3_1_2"/>
    <dbReference type="OMA" id="MLYPTDT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="10"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="212" mass="22975" checksum="EB33A6069532B30D" modified="2002-06-01" version="1">MVTVLEEEDPHAVKHVVRALRAGKLVAIPCDTVYSLSCDATNSEAVRRLYAAKERPKDKPVSVAVHSPEKALELLEPVPRLEAALETLTPGPVTIVAPRRPGVVAPEVAAGRRTLGVRIPDHPFFLRVVRRFGKPIVTTSANVSGRPAPTDPDEVFEQLGDRLDILVEGECRFGEPSTVIELTPEGNIDILREGAMPKEEILETLRGTTSGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-12-15" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN9</accession>
    <name>ARLY_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">MK0622</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXN9"/>
    <dbReference type="SMR" id="Q8TXN9"/>
    <dbReference type="STRING" id="190192.MK0622"/>
    <dbReference type="PRIDE" id="Q8TXN9"/>
    <dbReference type="EnsemblBacteria" id="AAM01837">
        <property type="protein sequence ID" value="AAM01837"/>
        <property type="gene ID" value="MK0622"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0622"/>
    <dbReference type="HOGENOM" id="CLU_027272_2_0_2"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137863" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="508" mass="55635" checksum="2838F8287E0ABE68" modified="2002-06-01" version="1">MALGRPSYRGGRVSGGRDPDFAELSSSLEQDREIFHCDVWNSVVHAVSLWEAGRIDRSTAAGIVEGLVTVLEEGPDRLPEDAEDVHEAIESRLHEVVGEEAGWLQLGRSRNDQVATSVRMRLRERALDLSRELVGLGRALLDLAREHAEVPIAGYTHLKRAQPCTIGFWMSTYAAAVARSARGLLRVPGMDECPLGCSAFPGSTVPVDRHREAALLGFRKPARHCGEATALRGPILEFLGRLATAASELTRLAGDLIQLCSDELGVVEPPDELSSTSSVMPHKKNPDALELVRAELTVVAGLKGLGDAVHGKLPMFYNRDLQVLNGLLWDSVNRFELCVRVLRKVVEGLDVDEEAARGTVLGSHAAAVDLAELVAERAGLTFREAHKVVGRVSARLDREGVPMSPERADRVVEELEREGVKLRVEEVREVLSLKRTLRRPVEGSTDPGRLEVTLDRLRAELAAAERLEGTWRGRIERALRATEAAVNRLGVEGFAEVYRGYWDGEAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP6</accession>
    <name>Q8TXP6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01829">
        <property type="protein sequence ID" value="AAM01829"/>
        <property type="gene ID" value="MK0614"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0614"/>
    <dbReference type="HOGENOM" id="CLU_1253592_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="220" mass="24578" checksum="A346110B59C9F15C" modified="2002-06-01" version="1">METVRISVLDVVRRIADVAALSVFEVIRDGPLDVLGCTVFNIELARRLVNKGLDVRLVSSEVGGPKGLPVVEEPASDTLLVTEPLTFYSEYHLLRSHALVLMFRYGNPGFKECLRGLDCTMLLPEIEGRPLPDLHTAFVAVLQEAVTSASEGHHVVGLRVSDTSDYFIPNSDPAEVKRRDDAIVVRAIPPYRLREGRVWEAVARAVDQVRFRRVSRCFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ8</accession>
    <name>Q8TXQ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Homolog of ABC-type nitrate/sulfonate/taurine/bicarbonate transport systems, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">MK0602</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01817">
        <property type="protein sequence ID" value="AAM01817"/>
        <property type="gene ID" value="MK0602"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0602"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.638"/>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_2"/>
    <dbReference type="OMA" id="LRWHPGY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="60"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="251" mass="27029" checksum="9179B8DCE57723C6" modified="2002-06-01" version="1">MIRNGDERLETLLKVSAPVAVLAIWQAVSGLGLINPVLLPPPSQVFSVFVEMPGEILKHALTSLYRVAVGYSIAAVAGVSLGVLMGTYRTAHAAMDLLIEIIRPIPPIAWIPLAIVWFGIGDPSAFFIIFVGSFFPILINTISGIRSVDRIYVEAALNLGADDTALIRHVLVPGALPNIFTGLRVGLGVGWMCVVAAEMIAAKSGLGYMIIEAQRILATDQVIAGMITIGLLGLVMDRGFRYLERRALVWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT8</accession>
    <name>HDRB_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase subunit B</fullName>
            <ecNumber evidence="2">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrB</name>
        <name type="ordered locus">MK0572</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXT8"/>
    <dbReference type="SMR" id="Q8TXT8"/>
    <dbReference type="STRING" id="190192.MK0572"/>
    <dbReference type="EnsemblBacteria" id="AAM01787">
        <property type="protein sequence ID" value="AAM01787"/>
        <property type="gene ID" value="MK0572"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0572"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.607"/>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="OMA" id="PGCVAQG"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150067" description="CoB--CoM heterodisulfide reductase subunit B">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="305" mass="34131" checksum="A23028DA7A976AAA" modified="2002-06-01" version="1">MAKKLCFFLGCIMPNRYPGIEKATRLVFEELGYELVDMDGASCCPAPGVFGSFDLKTWVTIAARNLSIAEEKGYDILTVCNGCFGSLNEANHLLQENPELREFVNEKLAEIDREYKGKVKVYHVNTFLYEEVGVKKIKEKVERPLEKTDGEPLKVAVHYGCHLLKPSEVTGFPGSVEDPRTLDELVEALGAESVDYKDKIMCCGAGGGVRSRELKVSLHFTREKIFNMLEAGADCTTNVCPFCHLQFDRGQIEMKEHFEKLPPKKLPVFHYCQLAGLAFGMDPEELALETHEIDCTPVLEKLGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU0</accession>
    <name>Q8TXU0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0570"/>
    <dbReference type="EnsemblBacteria" id="AAM01785">
        <property type="protein sequence ID" value="AAM01785"/>
        <property type="gene ID" value="MK0570"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0570"/>
    <dbReference type="HOGENOM" id="CLU_147797_0_0_2"/>
    <dbReference type="OMA" id="TPICGPY"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012029">
        <property type="entry name" value="UCP006557"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09884">
        <property type="entry name" value="DUF2111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006557">
        <property type="entry name" value="UCP006557_sign"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="116" mass="12852" checksum="9BC439F1AC7A09A4" modified="2002-06-01" version="1">MRIRPDSTAEDILPLAMAVHELVNRLPVTMRTRDNPGVRIEDGEVIDDEYTGPVLEEVLEKGEVIRKVPESGPYEGTPVVVVPIKDEGETIAALGVVDLTYGIYSSLRKVTQRPIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXV7</accession>
    <name>Q8TXV7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NhaP-type Na+/H+ or K+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nhaP</name>
        <name evidence="6" type="ordered locus">MK0552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0552"/>
    <dbReference type="EnsemblBacteria" id="AAM01767">
        <property type="protein sequence ID" value="AAM01767"/>
        <property type="gene ID" value="MK0552"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0552"/>
    <dbReference type="HOGENOM" id="CLU_005912_9_3_2"/>
    <dbReference type="OMA" id="QIGMFVL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="297"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="5">
        <location>
            <begin position="24"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="434" mass="46556" checksum="B950214B281C9F82" modified="2002-06-01" version="1">MWRTVKMGGSTTKFGFFTLVGYTALLLSLGAIVARLSEDRGLPDIPFLLLLGFLLGPIAGIVRPEYAQKAFPFVGTLGLIIILLDGGFEIGIDVLRRVASLVAKLDSITLLITAGISSLIFNLVFGLKPFSPIGFLYGSITCATDPATLIPVFSKVELPINISTALIAESVFNDPLGVVLTKMSLSVMGLSSHQNPILLFISLAAGGAALGLATGVVLERLLAREPFGEYVVPITLGAALALWYICEELLPGLLGYELSGFMAVAVLGMYLGNNLIKHDYLKDDRTFLKDFFEELSTVVRIMVFTLLGACVSISLLKTFWLKGLVCALSNVFIARPAGVIIGTYIPPKEDLNLKERIYLALEGPRGVVPAALVGTIYSKIVSNPHAVPVAIASEMPPKTLASAILVTTFLTIFISVVLEATWAQPLAKRLLKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXZ6</accession>
    <name>Q8TXZ6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Terpene cyclase/mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0511</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01726">
        <property type="protein sequence ID" value="AAM01726"/>
        <property type="gene ID" value="MK0511"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0511"/>
    <dbReference type="HOGENOM" id="CLU_646574_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="PFTB_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="424" mass="46944" checksum="9ABC3AB6343E942B" modified="2002-06-01" version="1">MVSPFSIQPRPPTPIMLIRGCGNTSLTHDKVLTFVVATLCLLGAAKADLVSDVQQRCEQAVDWLLTNQQPDGGWLYEGYGDKSSAMDTGVVLLGLCEAYDRLPQDQQCKVREAVDRGVEYILKCWDGDRKMFWDYPDGGGKEEYMPMYTVHALMGLIAVEKKFPDIAEKHGVSKYIDEALEKLLSMQNDDGSWSNFGPGSWSSPGRCTGLVVWLAEWSGRYGPSDERIAKGLKYIENHLEKSPLGGLISSNGPKIDFTVWDMLAFYFSGDEYYRSFVPKLQEGVLGFQHKDDEFAGAFAAHTSVYDSEYGPEGTAAPCPHKTARVLFALMLTGADPNDDRVKKAVEFLLNTKSQSEGAWFWPTMNDPSKPDNNPMKAYCTGWCLAALGAWLQKVPAKKHGIPVSPLMVALAFIPARRAWQAGTP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY00</accession>
    <name>NUCS_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease NucS</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nucS</name>
        <name type="ordered locus">MK0507</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY00"/>
    <dbReference type="SMR" id="Q8TY00"/>
    <dbReference type="STRING" id="190192.MK0507"/>
    <dbReference type="EnsemblBacteria" id="AAM01722">
        <property type="protein sequence ID" value="AAM01722"/>
        <property type="gene ID" value="MK0507"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0507"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.538"/>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="OMA" id="RCSVDYA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="PTHR38814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155692" description="Endonuclease NucS">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <sequence length="260" mass="29041" checksum="7E099024A5000C57" modified="2002-06-01" version="1">MTKVLVEPDPEEVKQLSEALGRQPVILAGICEAEYRGRAESVAGPALRIAMCKPDGTFILHNAMEKREPTNWNPAPSRQSIEVRDGCVVLRSRRLDVPEEVVVYFHKVLLACSLPKEGAKSEDSVFSLFRSEEDMKRVIREDPSVIEPGFRPVGEEVECGAGVADVVGYDEEGRFVVLELKRTRAGVSAASQLRRYVEAFREERGEEVRGILVAPSVTDRCRRLLEKYGLEWKKLEPVPLRDDGGKKQCTLTEFLAGEGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-02-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY21</accession>
    <name>MPTA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase MptA</fullName>
            <ecNumber evidence="1">3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptA</name>
        <name type="ordered locus">MK0485</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + diphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.39" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY21"/>
    <dbReference type="SMR" id="Q8TY21"/>
    <dbReference type="STRING" id="190192.MK0485"/>
    <dbReference type="PRIDE" id="Q8TY21"/>
    <dbReference type="EnsemblBacteria" id="AAM01700">
        <property type="protein sequence ID" value="AAM01700"/>
        <property type="gene ID" value="MK0485"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0485"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.515"/>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="OMA" id="PCSQGMS"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003933">
        <property type="term" value="F:GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00294">
        <property type="entry name" value="TIGR00294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147744" description="GTP cyclohydrolase MptA">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <sequence length="325" mass="37112" checksum="3120D8DAFA432F04" modified="2002-06-01" version="1">MRSCFPDVQSADPQIPAHLTRVGISGIKKLVKIPRRGKRSIVLVSTFNLFVDLPAHQKGIHMSRSHEVLQEVLEELEMSVEGSDTVIEDLCSRISRRLLERHDYATRSEVYMTGELILSRRTPVTKLPTQEPYKIIGRAVSKRTDNGIETRKMVGAEVVGLTACPCALEMMREHGKERVKHRLMEELDLEEEEAEDLAKKIVREDVHPMTHNQRGVGTILIEVSDVHRVSINDIIEIIEESMSAPTYELLKRPDELKVTCSACENPKFVEDCVREMIRRIVERFDYLPDDAVVIVRQVNKESIHKHDAFAERVTTMGELRKELGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY32</accession>
    <name>Q8TY32_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein, conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaD</name>
        <name evidence="2" type="ordered locus">MK0474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01689">
        <property type="protein sequence ID" value="AAM01689"/>
        <property type="gene ID" value="MK0474"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0474"/>
    <dbReference type="HOGENOM" id="CLU_2581433_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019213">
        <property type="entry name" value="DUF2108_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011308">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09881">
        <property type="entry name" value="DUF2108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006581">
        <property type="entry name" value="EhaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="80" mass="8508" checksum="B7841AC531C16300" modified="2002-06-01" version="1">MNWLLLTSLTACVLGSIATVLRRDPLQKLPSIALVKSGMICAVAAEGYLDVAAAGVALEAVGTIMFCVYLIRIEEVRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY42</accession>
    <name>Q8TY42_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ni,Fe-hydrogenase III small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ehaN</name>
        <name evidence="2" type="ordered locus">MK0464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0464"/>
    <dbReference type="EnsemblBacteria" id="AAM01679">
        <property type="protein sequence ID" value="AAM01679"/>
        <property type="gene ID" value="MK0464"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0464"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.494"/>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="OMA" id="QYGIYVH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="1">
        <location>
            <begin position="29"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="162" mass="17926" checksum="81F07FB7B319779E" modified="2002-06-01" version="1">MSLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKSDVLIVTGPVTYQWRDKLVKLYHKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPVDAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY50</accession>
    <name>Q8TY50_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="10" type="ordered locus">MK0456</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0456"/>
    <dbReference type="EnsemblBacteria" id="AAM01671">
        <property type="protein sequence ID" value="AAM01671"/>
        <property type="gene ID" value="MK0456"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0456"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.486"/>
    <dbReference type="HOGENOM" id="CLU_019597_2_0_2"/>
    <dbReference type="OMA" id="AEMKTGR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="190"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="105"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="221"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="242"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="309"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="423" mass="48729" checksum="77770E572ACAAE16" modified="2002-06-01" version="1">MPSRPRAVLVERLEWRQESRIDELKELAESAGYDVVGSFRQVRHEDPRYHIGEGKVKELAEFVRENDVDKVIFENEIKPVQAFNLAGELGVEVIDRFQLILEIFAQRARTREAKLQVKLAQLKYELPRAREMVNLAKKEERPGFRGLGKYEADKYEEMIRRKIAKIERELRRIEKDRELKRKHRHRLGFELVTLAGYTCAGKSTLMRALTDETVYVDSKMFSTLDTKTRAVDLDGHRVLLTDTVGFVDNLPHWLVESFKSTLEETAQADLVLLVVDVSDELPEIKRKLRVCHRTLEEIGAEGPIVTALNKADLIGWEEAERRLRELEGYVSHPVVVSAKTGEGLDDLKAEMRTVLSRYWKNVRIELPMRNETMRVVSKLHELGNVLDERWSNDGVEVFLEVSEKALGTVRGTVKGFGKVEVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY51</accession>
    <name>Q8TY51_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01670">
        <property type="protein sequence ID" value="AAM01670"/>
        <property type="gene ID" value="MK0455"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0455"/>
    <dbReference type="HOGENOM" id="CLU_143941_0_0_2"/>
    <dbReference type="OMA" id="INIAHHV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014514">
        <property type="entry name" value="UCP021940"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09974">
        <property type="entry name" value="DUF2209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021940">
        <property type="entry name" value="UCP021940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="140" mass="15371" checksum="FEB9F0DE976B708C" modified="2002-06-01" version="1">MNIVGVDVSGRHAEEDGHYNRITACVSAEVDGFNVFTVRDVNVFVVCTREPPNLRTLTEEVSRALNGLDKDDEYMVVAEPGEFFGEPEWRVSALLGAPFKYAETVAEREVVEMAHKLAYGVYRGVKMGLKVAQSPSGGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY95</accession>
    <name>Q8TY95_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01625">
        <property type="protein sequence ID" value="AAM01625"/>
        <property type="gene ID" value="MK0410"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0410"/>
    <dbReference type="HOGENOM" id="CLU_1821031_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036608">
        <property type="entry name" value="MTH777-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012033">
        <property type="entry name" value="UCP006600"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09001">
        <property type="entry name" value="DUF1890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006600">
        <property type="entry name" value="UCP006600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75181">
        <property type="entry name" value="SSF75181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="141" mass="15879" checksum="6395AB6FA3549617" modified="2002-06-01" version="1">MLVVSLGCPKIPPKISSAIYACYRYDDRALVLSTEAGCKLLEYADPEKEYVDETRTYEKYLEDPDAAGILLAFVTNDTELQIAVTLKEVSEPDDARAVCTEEFEDRLEDCGFLTAPVTGRRWKHDPKRWVKAVEDVLGASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA5</accession>
    <name>Q8TYA5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted RNA-binding protein containing the S4 domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01613">
        <property type="protein sequence ID" value="AAM01613"/>
        <property type="gene ID" value="MK0398"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0398"/>
    <dbReference type="HOGENOM" id="CLU_2597745_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="1">
        <location>
            <begin position="5"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="79" mass="8930" checksum="87B143AA16BAF785" modified="2002-06-01" version="1">MTVGRRLDAFLRDVGLAESRREAKRLVESGRVRVNGKLVRKPWWLVSPGDEIEVDGVTVRVEDNGGERRVSRIEGARSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYB9</accession>
    <name>Q8TYB9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Predicted hydrolase related to cellulase M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">MK0383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0383"/>
    <dbReference type="EnsemblBacteria" id="AAM01598">
        <property type="protein sequence ID" value="AAM01598"/>
        <property type="gene ID" value="MK0383"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0383"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.409"/>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_2"/>
    <dbReference type="OMA" id="FGWPAIH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001123-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="355" mass="38122" checksum="BA3D053156524B6B" modified="2002-06-01" version="1">MGTVETLTEHLRELVGRVAPPGWEDEVREYVEATLEKYCDDVHVDTLGNVIGTIEGSEYEVMVAAHMDEVGFIVKSIDKNGFIRFAKLGGIDDRILPGSRVIIVNSEGEKVPGVVGTKPPHIQEPKDRRKVPKHKDLFIDIGASDREEAEELVSVGDVGVLAGEFVELVGSRVNGRGLDDKIGVAVLLALAERLADLDGDHPTFYLVGTVQEEVGLKGAKTSAFEVYPDGAVVLDTAVAGDVPGVKEAELKLGKGPAITVVDASGRGLITHPKVRKLLIDTAEELDIPYQLEVGEGGTTDATAIHLTRGGVPTGVVGIPTRYLHSPAEVLDLEDAKHALELVVEVVQRFPDYVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD0</accession>
    <name>Q8TYD0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">UDP-N-acetylglucosamine:LPS N-acetylglucosamine transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">murG</name>
        <name evidence="5" type="ordered locus">MK0372</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="CAZy" id="GT28">
        <property type="family name" value="Glycosyltransferase Family 28"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01587">
        <property type="protein sequence ID" value="AAM01587"/>
        <property type="gene ID" value="MK0372"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0372"/>
    <dbReference type="HOGENOM" id="CLU_037404_2_1_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_transf_28" evidence="3">
        <location>
            <begin position="3"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_tran_28_C" evidence="4">
        <location>
            <begin position="186"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="356" mass="38597" checksum="4C79629D779011B4" modified="2002-06-01" version="1">MHVLIASGDGGHLTRALALAEELSDRGHDVTFAVNEDSDQAVERLKKAGFEEYVGLPRPRRMGDTSWKAALGGLKNYLAASKVLRDVRPDLILSTGAGVAIGPMIAGKFKRLPVAHYEPTDVVSVSGKVAKLCADIIGVWDEDMAEYYGDRVINVGIVLPRSFEEADPEEAREKYDLGDRVLVWTTGSAGYKPALEGLVRCAEEGLLRDWEVVVNTGNAMDPKRLKRALNGLCSGIVVKRFFHDFPALLKAADLVVCLGGATPVEAAALGKPVVVLPRRDVLRDHQYVTAKKLEKRGVAVAAEDASNPEEVVKAVSRALSIDPEDLKRMGERGKELFGGNARERFIDLCEELVASG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-12-04" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYD4</accession>
    <name>DADD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="1">5'-dA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="1">MTA deaminase</shortName>
            <ecNumber evidence="1">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenosine deaminase</fullName>
            <ecNumber evidence="1">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="1">SAH deaminase</shortName>
            <ecNumber evidence="1">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dadD</name>
        <name type="ordered locus">MK0366</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:42893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:20717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:25026"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.41" evidence="1"/>
    <dbReference type="EC" id="3.5.4.31" evidence="1"/>
    <dbReference type="EC" id="3.5.4.4" evidence="1"/>
    <dbReference type="EC" id="3.5.4.28" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYD4"/>
    <dbReference type="SMR" id="Q8TYD4"/>
    <dbReference type="STRING" id="190192.MK0366"/>
    <dbReference type="EnsemblBacteria" id="AAM01581">
        <property type="protein sequence ID" value="AAM01581"/>
        <property type="gene ID" value="MK0366"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0366"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.388"/>
    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
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        <source>
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    <sequence length="431" mass="47752" checksum="2B0B0BB50BCDB1B8" modified="2002-06-01" version="1">MRLAILGGIAVTPERVIEDAGILIDEDGRISFVDTREQLEECEDWEDEIELGEKDVIMPGLINTHTHGPMTLFRGVADDMPLMKWLREEIWPLEERLDAEKCRWGAALAAMEALKSGTTCLADMYFFMDAVAEAYAEVGIRAVISHGMIDLGEEDKREEELKESKRVYRKCQGMEGLIEFSLGPHAPYTCSEELLKEVRRLADEWGVKIQIHVAETEDEVKEVKRKHGKRPVEYLDEIGLLGDDVIAAHCVWLDDKEIEILSKRGVIVSHNPISNMKLASGISPVPEMLERGVNVTIGTDGCASNNNLDMLEEIKVAALLHKVNKMDPSATEMLEILRMATVRAGTVFSSEKIGAIEEGYAADLVVLDGSSPRLNPNHNPISNIVYSASGSDVKHVFVAGELVVKNGKLVKADEQEILENSTECAEQLTSS</sequence>
</entry>
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        <submittedName>
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        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="380" mass="41927" checksum="0AA868AAB9FA90D9" modified="2002-06-01" version="1">MPTVVRIDEFWDMTEGERRSVDAVTCATVGLMSGVYGILSFHVAEPGEVRRFEEVYLEGIRLPVGPCPNERLGLVECVVPATAHGDVSGAYLLRTVAEGNEFDVIAVADDGREYEATIGPEELERAMIASTRACFRNYSAIFNDGERPASTIFAPRPLEPGEASFSGCGGYNPLEHDPKLRLHRPGRRVLFCGAPGVITGKGTRSTRERPNLTLHANLTECDPTFMGEYRTSAGPENIAGVASVIAVTEETLPFLERDHSDAVLPVVKLSDRTPIAETTYADVWVQETVRWNPDRCENCDPCRLEEKCPYPYGDLRTGDCFHCGYCHEFCPAVEVDLGELEVEGRKLPIVARESSAYLAKKLMERAIEAVEEGKIDILRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYL8</accession>
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        <submittedName>
            <fullName evidence="2">Predicted membrane protein, family MK-41 family</fullName>
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        <name evidence="2" type="ordered locus">MK0278</name>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="MK0278"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="670" mass="74379" checksum="75326D29C41368B2" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYM9</accession>
    <name>Q8TYM9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Coenzyme F420-reducing hydrogenase, gamma subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0267"/>
    <dbReference type="EnsemblBacteria" id="AAM01484">
        <property type="protein sequence ID" value="AAM01484"/>
        <property type="gene ID" value="MK0267"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0267"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.270"/>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="OMA" id="VKIATTW"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="18"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33056" checksum="A8EE5B305AA22BAF" modified="2002-06-01" version="1">MKGVVRLVKIATTWLCCCSGCHVSVLDLHERLLDLLGDAELVHCPVLMDTKEIPEDVDVVLIEGGIRNEENVEVAEEFRERAEIVVAVGTCACYGGVPGLANLYSNEELLRTVYVETASTENEDGVIPSEDVPELTWRVRPLSDVIDVDYELPGCPPEPDLIADAVTAILDGREPELSTTNLCEECPRKKEETVIHEIRRPVEGEPDPDRCLLEQGYPCMGPATRAGCGARCPEAGVPCAGCAGPAGEIPDQGAEMMSAIASIFRADVDDVDPSELVESVPDVVGWFYRFTLAGSLMPFRVDRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYN8</accession>
    <name>EF1B_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 1-beta</fullName>
            <shortName evidence="1">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ef1b</name>
        <name type="synonym">efb1</name>
        <name type="ordered locus">MK0255</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYN8"/>
    <dbReference type="SMR" id="Q8TYN8"/>
    <dbReference type="STRING" id="190192.MK0255"/>
    <dbReference type="EnsemblBacteria" id="AAM01472">
        <property type="protein sequence ID" value="AAM01472"/>
        <property type="gene ID" value="MK0255"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0255"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.258"/>
    <dbReference type="HOGENOM" id="CLU_165896_1_0_2"/>
    <dbReference type="OMA" id="VMPNSPE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="PTHR39647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="SSF54984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155059" description="Elongation factor 1-beta">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <sequence length="94" mass="10298" checksum="A0BA4AF172553B2C" modified="2002-06-01" version="1">MIVVSKVLVDLKVLPESADVDYEELKEAIREKLESMDVVDIEGMEEEPIAFGLKAIRVKVVVPDAEGGTDALEDALKEVDEVNQVEVVSASRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ9</accession>
    <name>Q8TYQ9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted dinucleotide-binding enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0233"/>
    <dbReference type="EnsemblBacteria" id="AAM01450">
        <property type="protein sequence ID" value="AAM01450"/>
        <property type="gene ID" value="MK0233"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0233"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.234"/>
    <dbReference type="HOGENOM" id="CLU_076368_1_0_2"/>
    <dbReference type="OMA" id="ARYNEDM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="1">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="223" mass="23975" checksum="3C379224E4AF6F92" modified="2002-06-01" version="1">MRLAFIGGTGHQGFGLALRLAAAGHHVIIGSREEERAVKAAEEAEEILAEHGYEDVTVEGRENSDAAAEAEVVFLTVPFFAVIDTVKAIRDSLDEDAIVVDVTVPLETAVGGKPTRLIRPWAGSAAETVQSLVNNPVVSAFENVSAESLRDLEKEVKCDVVVCSDHEDAKRTVMELAEEIPGVRAIDGGPLENARIVESITALLISLNMRYGKEDVGIRFTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2020-12-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT8</accession>
    <name>Q8TYT8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Single-stranded DNA-specific exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recJ_1</name>
        <name evidence="1" type="ordered locus">MK0204</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01421">
        <property type="protein sequence ID" value="AAM01421"/>
        <property type="gene ID" value="MK0204"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0204"/>
    <dbReference type="HOGENOM" id="CLU_808009_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="343" mass="37221" checksum="144C284367983DDD" modified="2002-06-01" version="1">MKLYPLVRKVLKLLSSADSIGIMADTDADGVSGAATLCEAFGVAEDDVWFPSGGFYGVPKDALRDMLEDYDVVVTVDLTPPPFPEARDRVVVIDHHPTDSHYPMTVNPYEIPALPTHTSASALVGMLAHRTGGLNRPWVPLIGAAGDGMEGGAVYETLASMTDPGYLVPSRGRNLTPLQDAAATVNAARRVKYDVGAREALKVLMSADSPYDVTESHLQRYRKRVRKHRAIWSGEAERTAHIVEGVGYAEIHTEVDVEGLVARDLVDRLNLRCSVVVNLARKPCGLYKASGRSQGFPVNELMVKMARWLEGSKAGGHPNAAALHFSDGDPREAFNRALDLLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYU5</accession>
    <name>Q8TYU5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Triphosphoribosyl-dephospho-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">citG_1</name>
        <name evidence="1" type="ordered locus">MK0196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0196"/>
    <dbReference type="EnsemblBacteria" id="AAM01413">
        <property type="protein sequence ID" value="AAM01413"/>
        <property type="gene ID" value="MK0196"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0196"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.197"/>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="OMA" id="GNTHFGA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="298" mass="33253" checksum="E290CB76B95FBB33" modified="2002-06-01" version="1">MVNEPERIAVSLTLGALLEVSSWPKPGNVHRTRDFDDTRFEHFLASAVAAQPVLREVAEMATRGERVPLGRYLYEAVRHSMSAHTGGNTNLGILLLDVLLASALARASLDPSDVRREALKLAKETEERDAYYLYRAIRLAGAGGMRRIRGSRAPDVSRPEDVLEKGITMYEALQAAAHRDAVAEDWVRGLERSLRIGLRVIELREEYDINEAVVRTFLEELATRPDTLIWRKHGFRVALRVSEAAQEILRIAGDRPVTETRALYELDRELHEDGINPGSTADLLAAGVGYACYLGMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ04</accession>
    <name>Q8TZ04_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0137"/>
    <dbReference type="EnsemblBacteria" id="AAM01354">
        <property type="protein sequence ID" value="AAM01354"/>
        <property type="gene ID" value="MK0137"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0137"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.136"/>
    <dbReference type="HOGENOM" id="CLU_017233_1_0_2"/>
    <dbReference type="OMA" id="FPIHKDL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd18086">
        <property type="entry name" value="HsC9orf114-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="PTHR12150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="255" mass="29504" checksum="3B978296D736951F" modified="2002-06-01" version="1">MVAVAFPWSLFSEETDPKIYAYRVGTLARALAIYRVEEVYLYGDGVGTRRNAERLRKLLEYQECPQYLRKRVFRLDRDLRYAGVMPPLRAPHHKVHSPKEGEVREGYVVRRSRNGALVDVGADRLARTRWRFKPHERVTVRIVSEDPLEVEPAEPEEYWGYRVRIVNELNEVLREFKEGIIVTSRYGEDVREVEFKSPVKCLVFGSSEVSVLDVDPGVRDEYPVINFVPNQGVQVVRTEEAVHTTLAVLNYLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ05</accession>
    <name>Q8TZ05_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01353">
        <property type="protein sequence ID" value="AAM01353"/>
        <property type="gene ID" value="MK0136"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0136"/>
    <dbReference type="HOGENOM" id="CLU_1976530_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012906">
        <property type="entry name" value="PaaX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07848">
        <property type="entry name" value="PaaX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PaaX" evidence="1">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="126" mass="14346" checksum="299649EF3C16338E" modified="2002-06-01" version="1">MSEVPEEFQKDVEEIKRRLREGELTNLGAALAVVLLASLKGQWINVDEIVRVLKELGYNVKANSIRSALYKVRQEGLLKSKRLGRKTAYFIPVDDDETLGAILRRVTGEEVREKIVEELLEEIHGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ40</accession>
    <name>Q8TZ40_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01316">
        <property type="protein sequence ID" value="AAM01316"/>
        <property type="gene ID" value="MK0099"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0099"/>
    <dbReference type="HOGENOM" id="CLU_2067824_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027392">
        <property type="entry name" value="TF_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13453">
        <property type="entry name" value="zf-TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-TFIIB" evidence="1">
        <location>
            <begin position="2"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="118" mass="13809" checksum="E4AAA1C5D0C698D7" modified="2002-06-01" version="1">MKCPECGTEMRSELRDDGVELLRCPECGKEIKRVPSYREFGLKCPNFFEMTVPEWDEEVLGKEVVVVVKYRSPDGYRRRKIRGKAVRIDNRGNLVIRREDGFEVSLYPQVVENIKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ55</accession>
    <name>Q8TZ55_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fe-S-cluster-containing hydrogenase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01301">
        <property type="protein sequence ID" value="AAM01301"/>
        <property type="gene ID" value="MK0084"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0084"/>
    <dbReference type="HOGENOM" id="CLU_1529237_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12798">
        <property type="entry name" value="Fer4_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="175" mass="18838" checksum="6D2736A7572F6FDD" modified="2002-06-01" version="1">MEGARREGVKAWVGLPGCIGCVLCAEACPIGAIEIVDGRPQKCIHCDPERAMCARACPHHAIVQVCETLVVDRDRCNGCGKCAEACPVGGIFIREDVAVKCDNCLDRDYPACVEVCPVAGADVAPVNERVLWRRSRAARTLRNLPGSGGHARRLGSRAARKHSRSHSRSVARGRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ81</accession>
    <name>RPO11_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo11</name>
        <name evidence="1" type="synonym">rpoL</name>
        <name type="ordered locus">MK0058</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ81"/>
    <dbReference type="SMR" id="Q8TZ81"/>
    <dbReference type="EnsemblBacteria" id="AAM01275">
        <property type="protein sequence ID" value="AAM01275"/>
        <property type="gene ID" value="MK0058"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0058"/>
    <dbReference type="HOGENOM" id="CLU_2140209_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149327" description="DNA-directed RNA polymerase subunit Rpo11">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <sequence length="112" mass="13024" checksum="C43CDB05BD988934" modified="2002-06-01" version="1">MKLPEVEVVVKKYDKDEVLLELPGEDHTLCNLLRWALNRQDGIIATYRIEHPILGKEHKVDEERYVPPKMRIRAVDEDADAREALERAIEELLELVEEAKEEFSGALEEKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ87</accession>
    <name>Q8TZ87_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATPase of the PP-loop superfamily implicated in cell cycle control</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0051"/>
    <dbReference type="EnsemblBacteria" id="AAM01268">
        <property type="protein sequence ID" value="AAM01268"/>
        <property type="gene ID" value="MK0051"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0051"/>
    <dbReference type="HOGENOM" id="CLU_830567_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP_bind_3" evidence="2">
        <location>
            <begin position="78"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="305" mass="34660" checksum="0B2BBE980116FA47" modified="2002-06-01" version="1">MDVEEILRDVGVDPRYVAILDDTVVALNPRRAGLTVSRREELRREGIKVVRSKVVERASSRFHQVVTDYDLFEPREEVVVGFSGGKDSVTCLLMLEPLTRRLGLRIRPVLVETRLHGDPIWGREGREVCEEICRSLGFQLEYVEPREDVGELAEEHGESPCLICSLIRRRELRERGDKLVLAHTLDDAIVTAMASAIKGEGLNILRPVEELGGMRSEYVDYDFPETTIVRPMIRVPEVWTRLIPGEVGLPIFESDCPYSKPYGTTLRGKVAHGLEWLRLEVGADSVEFLDRLYRSFMKTLEATRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC5</accession>
    <name>RFCL_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Replication factor C large subunit</fullName>
            <shortName evidence="1">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rfcL</name>
        <name type="ordered locus">MK0005</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZC5"/>
    <dbReference type="SMR" id="Q8TZC5"/>
    <dbReference type="STRING" id="190192.MK0005"/>
    <dbReference type="PRIDE" id="Q8TZC5"/>
    <dbReference type="EnsemblBacteria" id="AAM01222">
        <property type="protein sequence ID" value="AAM01222"/>
        <property type="gene ID" value="MK0005"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0005"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.5"/>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="OMA" id="GGWTRYG"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135953" description="Replication factor C large subunit">
        <location>
            <begin position="1"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="510" mass="58255" checksum="E3676E6348A8DED2" modified="2002-06-01" version="1">MVPWVEKYRPRSLKELVNQDEAKKELAAWANEWARGSIPEPRAVLLHGPPGTGKTSAAYALAHDFGWDVIELNASDKRTRNVIEKIVGGASTSRSLLRMTREAGGDYEHVEGHSDRVLVLVDEVDGIDPREDRGGVTALTRAVRQARNPMVLVANDPWVLPKSLRDAVRMIEFRRLRVNDIVEALRRICEREGIEYEEVALRRIAKRARGDLRAAINDLEALARPTGRVTSDDVEALGWRDKEITIFEALGRIFNKPPRQARRALWNLDEDPDDVILWIAQNIPRAYRDPEEIARAYDYLSKADVFSSRAIETGDWRFKYVYATDLMTSGVAAARKGKPPGFVRFQPPKILRKLGTTRKEREVRNSIAKKIAERMHVSTRRAKMDVISVLEIAFRKVADNPTDRGLEILGGIAGYLELSKREIGFLCGDPQVAQRVYQRALRVREKLRKIRRERVKGAMESMLERKREESEVEEEAKEIEEAVEKAEEEEEREEKKKEGGGEQRTLDAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC8</accession>
    <name>Q8TZC8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molecular chaperone (Small heat shock protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ibpA</name>
        <name evidence="4" type="ordered locus">MK0002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TZC8"/>
    <dbReference type="STRING" id="190192.MK0002"/>
    <dbReference type="EnsemblBacteria" id="AAM01219">
        <property type="protein sequence ID" value="AAM01219"/>
        <property type="gene ID" value="MK0002"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0002"/>
    <dbReference type="HOGENOM" id="CLU_1559495_0_0_2"/>
    <dbReference type="OMA" id="INDQWHR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="62"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="18996" checksum="164C8B877486E803" modified="2002-06-01" version="1">MGVIEDMMKVGMRSAKAGLEATEELIKLFREDGRLVGSILKEMEPEEITELLEGASSQLIRMIRSLHTPAVDVFERSGEFVIVAEVPGARPEDVQVRAGERFVEITANIPKMREGEAKTRERVTGEVRRRIDLPEKINPSAVSAKCGRGLLIVRAPKAEASEIEVKPMEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-05-03" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>F1SVH1</accession>
    <name>F1SVH1_METKA</name>
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        <submittedName>
            <fullName evidence="6">Reverse gyrase, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rgyB</name>
        <name evidence="6" type="ordered locus">MK0049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
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            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
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        <property type="protein sequence ID" value="AAM01266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAM01266">
        <property type="protein sequence ID" value="AAM01266"/>
        <property type="gene ID" value="MK0049"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0049"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.49"/>
    <dbReference type="HOGENOM" id="CLU_002886_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003916">
        <property type="term" value="F:DNA topoisomerase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
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        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
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        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
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        <property type="entry name" value="PTHR43505"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
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    </dbReference>
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        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="165"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="424"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="3">
        <location>
            <begin position="779"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="335"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="1221" mass="138112" checksum="DF930C7976CC723C" modified="2011-05-03" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGY4</accession>
    <name>COAD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="1">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaD</name>
        <name type="ordered locus">MK1504</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TGY4"/>
    <dbReference type="SMR" id="Q8TGY4"/>
    <dbReference type="STRING" id="190192.MK1504"/>
    <dbReference type="EnsemblBacteria" id="AAM02717">
        <property type="protein sequence ID" value="AAM02717"/>
        <property type="gene ID" value="MK1504"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1504"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1662"/>
    <dbReference type="HOGENOM" id="CLU_035272_5_0_2"/>
    <dbReference type="OMA" id="FDTLHSG"/>
    <dbReference type="UniPathway" id="UPA00241"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156321" description="Phosphopantetheine adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <sequence length="157" mass="17612" checksum="7F26ACA3277445F6" modified="2002-06-01" version="1">MTPLARFRKVVVGGTFDRLHLGHQRLLSVALELGDRVVIGVTTDSFVREEGKKGVEPFEERVRAVRRFVEEKGASDRVEIVPLEDRYGTTLEDDEMDAIVVSPETEPVALEINELRRKRGFPPLSIVVIPFVLDGDGRKISSSRLRGEVDEGPCRDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-10-11" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TGZ7</accession>
    <name>ENDA_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="1">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">endA</name>
        <name type="ordered locus">MK0341</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="5X89">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.53 A"/>
        <property type="chains" value="A=2-179"/>
    </dbReference>
    <dbReference type="PDBsum" id="5X89"/>
    <dbReference type="AlphaFoldDB" id="Q8TGZ7"/>
    <dbReference type="SMR" id="Q8TGZ7"/>
    <dbReference type="STRING" id="190192.MK0341"/>
    <dbReference type="EnsemblBacteria" id="AAM01556">
        <property type="protein sequence ID" value="AAM01556"/>
        <property type="gene ID" value="MK0341"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0341"/>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="OMA" id="KGPGIDH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="SSF53032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="SSF55267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000109471" description="tRNA-splicing endonuclease">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="13"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="20"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="28"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="36"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="42"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="47"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="71"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="110"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="155"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="5X89"/>
        </source>
    </evidence>
    <sequence length="179" mass="20939" checksum="AC84893E8167E5D5" modified="2002-06-01" version="1">MLCAGNGGKELPRAKVFEGGSLVSKDYEDLKRRYFGTEHGNVLFLDPFETVYLTEKGEIDPETPEGEPMSVEELLSFFERRRPGFRAGYVVYRDLTERGYVVKSGFKYGGRFRVYEEDPDREHSKYVVRVVEPDTELSTRDVLRATRLAHSVRKDFVLAVVEDVEEPRIEYVMWRWKRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUU1</accession>
    <name>PGK_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="1">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk</name>
        <name type="ordered locus">MK1662</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TUU1"/>
    <dbReference type="SMR" id="Q8TUU1"/>
    <dbReference type="STRING" id="190192.MK1662"/>
    <dbReference type="PRIDE" id="Q8TUU1"/>
    <dbReference type="EnsemblBacteria" id="AAM02875">
        <property type="protein sequence ID" value="AAM02875"/>
        <property type="gene ID" value="MK1662"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1662"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1826"/>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146059" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="357"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <sequence length="406" mass="45132" checksum="8C99B1D4C0C3FABD" modified="2002-06-01" version="1">MYENISTMDDFEFENKWVLLRIDINSTVIDGKIEDDERIKRHLGTIKELMEHDARVAILAHQGRPGEDDFTTLEPHAEIMSEELDNFEYVPDVFGPTAKKKIRSLEPGEVILLENVRFYSEERINRDPEWHARRHLVRNLAPLFDIFVNDAFAAAHRSNASLVGFTRRLPSCVGRVMEREIEVLETMVRDEMEDGVFVIGGSKIEDAIKVIRRAIEMDNVRRVLLGGLVGNLFLWASGVDLGKPSRKFLDMKGYTGYLDEARELLEEGDDVILVPEDVALNRGGEREEVDVDELPADAPVFDIGTGTIERYRKEVESAGMVVANGPMGVYEEPGFEKGTYEVLNAIADSEAFSVIGGGHIIAAAKACGAYDSIDHVSTGGGAMLRMLAGERLPAIDAILTCPFSGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TUW6</accession>
    <name>Q8TUW6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Orphan DOD family homing endonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1637"/>
    <dbReference type="EnsemblBacteria" id="AAM02850">
        <property type="protein sequence ID" value="AAM02850"/>
        <property type="gene ID" value="MK1637"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1637"/>
    <dbReference type="HOGENOM" id="CLU_1253594_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="1">
        <location>
            <begin position="50"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="220" mass="24573" checksum="9407C2157400E9F0" modified="2002-06-01" version="1">MTSSFYGEPEGLRWLEDDLRALGLSPVRYEYGDRDAVEVAVHSEGFAALLRGLGMPEGRKTGRVHVPGLVRRGPDRVALEFLSGLFGADGWISARENRVEVGIAQASPWGESSEFLEGVSSLLKRTTGVYARVLEAGSYETSVDGRRSVFGLGFRGEHVERFLTRVPFEYSVRKRELGLWAGAYLRYRARGGKDAFERFVEERCLPGGLVLDRVVDVERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV33</accession>
    <name>Q8TV33_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1567"/>
    <dbReference type="EnsemblBacteria" id="AAM02780">
        <property type="protein sequence ID" value="AAM02780"/>
        <property type="gene ID" value="MK1567"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1567"/>
    <dbReference type="HOGENOM" id="CLU_030571_2_6_2"/>
    <dbReference type="OMA" id="KNIVTGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039344">
        <property type="entry name" value="MBLAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23200">
        <property type="entry name" value="PTHR23200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="24"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="183" mass="19745" checksum="5FE3E56E8AC17E81" modified="2002-06-01" version="1">MTVVVVVRKGRLVRSASGGVLEADSSSTLILTEEMNLLVDTGGEDPKGLIRDLEPYGVDAVVFTHGHQDHVGNARAIAETFHPEFYAHEREAGSIPVDVDPVDSFDPPEGVEVIETPGHTPGHISVVVEDRIVIAGDACPTPDNALERVPPAISWDRELAEESLRKVLSYPVVIPGHGIHLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV47</accession>
    <name>Q8TV47_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted allosteric regulator of homoserine dehydrogenase containing an ACT domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1553"/>
    <dbReference type="EnsemblBacteria" id="AAM02766">
        <property type="protein sequence ID" value="AAM02766"/>
        <property type="gene ID" value="MK1553"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1553"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1713"/>
    <dbReference type="HOGENOM" id="CLU_094842_1_0_2"/>
    <dbReference type="OMA" id="ERGTIPV"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="171" mass="19100" checksum="A7E38D4947DF6B6C" modified="2002-06-01" version="1">MKRFELDVVLPDKPGQLVKVLEPLSKIGGNVISISHSRDGDRARVHIVFEATEDVAREYSRRISELEGVKILRFGRGPGHETDVVLIGHIVDTDIKDTIDRVNAIQGARVVDVDLEMPDPERESSAGFTLIYEDEEALRKAVQTIEEIAEEKDLVAIFPVEVIQCVRRASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV68</accession>
    <name>Q8TV68_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Formylmethanofuran dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fwdC</name>
        <name evidence="6" type="ordered locus">MK1530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FwdC/FmdC family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1530"/>
    <dbReference type="EnsemblBacteria" id="AAM02743">
        <property type="protein sequence ID" value="AAM02743"/>
        <property type="gene ID" value="MK1530"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1530"/>
    <dbReference type="HOGENOM" id="CLU_072248_0_0_2"/>
    <dbReference type="OMA" id="VNGDAGM"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GXGXG" evidence="5">
        <location>
            <begin position="87"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM02743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="267" mass="28405" checksum="D7304D1D85B44042" modified="2002-06-01" version="1">MKEVVLTPKGEPDVPLEAEVICPDEFAGKSEGEIEALKIYEGNSTVELGEYFDVEGDAGDSPGDTRIVIEGDVPWVKLIGYRMSAGEILVKGDVGRHAGAEMKGGKLIVEGDADDWLGREMKGGEITVHGNAGNYVGSTYRGEWRGMSGGRILVKGDAGDEIGEWMSDGKIIVEGDAGIMIGIHMQGGTIIVRGDVGVRPGAQMEGGTVVVCGRAEDILPSFRYEGLKEDPVEEATGTFHLFTGDYANGPKVEGELYLSTALNEVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TV88</accession>
    <name>Q8TV88_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ATPase of the PP-loop superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1505"/>
    <dbReference type="EnsemblBacteria" id="AAM02718">
        <property type="protein sequence ID" value="AAM02718"/>
        <property type="gene ID" value="MK1505"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1505"/>
    <dbReference type="HOGENOM" id="CLU_1048119_0_0_2"/>
    <dbReference type="OMA" id="YHPCGRC"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012096">
        <property type="entry name" value="ATPase_PP-loop_MJ1638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006601">
        <property type="entry name" value="ATPase_UCP006601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="265" mass="30452" checksum="5481A30B10DE8892" modified="2002-06-01" version="1">MVVQAWTDLKVRLLRLDASREILERYSRRQPELEGVLRGIEIEKARLHGELPWDYWERWEGEEPRDMRITVAASGGYDSTASAIILRKLGYEVESVTVDPGPMFLPPKLRRNVEILSEYLGTEPKFVEEDLSDVVEGALNEGRFHPCGRCNAKIRKRVMGEADTDVVAFGDGLPTGHHCVQPEDDRFKLHVPAALAKTKFELRRLVEEVLPEFHDYKYACPFLHQVHQRHPHLRRASIQRVLRELRHGFLEVGEALKAVYDILGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-28" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVD1</accession>
    <name>RS3A_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3ae</name>
        <name type="ordered locus">MK1461</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TVD1"/>
    <dbReference type="SMR" id="Q8TVD1"/>
    <dbReference type="STRING" id="190192.MK1461"/>
    <dbReference type="EnsemblBacteria" id="AAM02674">
        <property type="protein sequence ID" value="AAM02674"/>
        <property type="gene ID" value="MK1461"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1461"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1617"/>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="OMA" id="GQNAYTK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="30S_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_S3Ae_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153549" description="30S ribosomal protein S3Ae">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="211" mass="24376" checksum="10EA971749AFA491" modified="2002-06-01" version="1">MVRDKWKDKVWYTILAPDMFDNVEVGETPADDPEKVIGRVLETTLGDVLDDITKHHIKVFFRIYDVEGTTAYSKFEGHRLMRDYVRSLVRRGTSRIDGVIDVVTKDGYKVRVAGLAFTTRRAKTSQQRAIRKEMFKVIEENAKECDFDEFIRRCLSISEEESIPEQIKEAGRKIYPIRQAEIRKTEVLEEPNGLPPYEAVGDRATPELASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVG6</accession>
    <name>Q8TVG6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02636">
        <property type="protein sequence ID" value="AAM02636"/>
        <property type="gene ID" value="MK1423"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1423"/>
    <dbReference type="HOGENOM" id="CLU_961764_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="289" mass="30485" checksum="9F34178D2E6366FB" modified="2002-06-01" version="1">MRRVLVVLSIALMALAAPGLAEDKAVTATTEQQSQQAQTAEQQTEQQATAPAPKTVTLKLTPSLQADFTVDLKNGEAVLTLTGGYSLVTEGQDLLSDVLTAALAGAINTIVAPIDGKELLPGVKLEAGVEAPTVTAGLGEDCGVTLTATVLKVEVPKLEDLEHEWSETVTEDPLKSGISVALRLLHTAEEPFIKATLDLLTGALKVVVHLFELEVGSPSEDYEGPYLELTPVNVSEKLNDNATVTAYLGASLSASTLKLSPTITYETTTPLKLPVTLPEITLYTFQLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVT7</accession>
    <name>Q8TVT7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Terpene cyclase/mutase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02514">
        <property type="protein sequence ID" value="AAM02514"/>
        <property type="gene ID" value="MK1301"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1301"/>
    <dbReference type="HOGENOM" id="CLU_484536_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="SSF48239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="562" mass="61459" checksum="14E60CF42BFD5F9A" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVU0</accession>
    <name>Q8TVU0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1298</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02511">
        <property type="protein sequence ID" value="AAM02511"/>
        <property type="gene ID" value="MK1298"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1298"/>
    <dbReference type="HOGENOM" id="CLU_616256_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010165">
        <property type="entry name" value="CRISPR-assoc_prot_TM1793"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019117">
        <property type="entry name" value="CRISPR-assoc_protein_Cmr3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09700">
        <property type="entry name" value="Cas_Cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01888">
        <property type="entry name" value="cas_cmr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="444" mass="47912" checksum="BFA34FB545C6D47C" modified="2002-06-01" version="1">MTARHVAVGFLVLPQDRAFFRRPEPFRAAAESVAESEGPKPWTVAGCVRNALLEVTDGDLGEVLEYPRKLVRSAPPGSIGEALGETALFLGTWVVEVRPGAPDRLERVWWPCPAHLARYEPESGGEAVDLLRVEEVGDAAIAFSGHDEALRRRYGFMTPKPPRPGSDVETPGRYVDTGTISKILAGRTPARRTPDLEDNVAGPGELLRPELHVGVKLVGLKTADEGYLYAARFLRPGRSPVDGGSLVGFLTVLAVPEDAWDDGVLEDLPSPGGRLGRGRHAWVIPLRAGDWSDDVFPEVEEPEGDLPGLYLETPTPFPEGDVRSSSPGDRLKAEAVLVRPGSPRDRVLQVELEVSEVESEVLADRVIETRRVSTWSGKRASPDHLAAAEGTVLALGKLDVDPGTPLLLFPRMGDEDRELLASRLAFHLSGVGTAYALSLPGVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVW9</accession>
    <name>Q8TVW9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-1 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1269</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02482">
        <property type="protein sequence ID" value="AAM02482"/>
        <property type="gene ID" value="MK1269"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1269"/>
    <dbReference type="HOGENOM" id="CLU_114818_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012465">
        <property type="entry name" value="DUF1678"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07913">
        <property type="entry name" value="DUF1678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="150" mass="17814" checksum="7DD9C4A797222291" modified="2002-06-01" version="1">MAHWRNSRHERGRTLYLGKSENESVSFIEYLVSLNRAEVLELARHLMRNLRSVLKSLLPQVSSLPYKKARRVLARGLALAFDARPAESPRIRDLLEELPDRLESFFMRTLGGWPAYYFSHLRKVIRSRRGSLDEKHEIPDVKLERWKFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVX2</accession>
    <name>Q8TVX2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-37 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02479">
        <property type="protein sequence ID" value="AAM02479"/>
        <property type="gene ID" value="MK1266"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1266"/>
    <dbReference type="HOGENOM" id="CLU_631081_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="434" mass="48523" checksum="682E886F6A73C02E" modified="2002-06-01" version="1">MSPGGVPVPERVPLSPTEEFYDRVKSYVERRMSDLGLEPPEDNESLLYDEKYGHFWTFNGFGRELWSDLSAVWILIVLPSNGLLSENIFELEEQPSTGKVLRDEVERVGRGLLERESKRHFSDYFLGELEVFKEASQHVKRFVLDEPNFEVAVNLWIAGAALASQDGTPRIAIQAISSIDTEVSEFPVGVPSYPLGYILQGCGITASVLCLTAHAMVNRGVEEDQLLEYLSDTPYGEKVRIICASWVEGTYKEVIKECLEGLKDGLNRLLRKGRGLNMRDLGIDDPEEFAHEVLRKLADVIANISAVFETVHREPERIVHTLLSVDEEIRLGDKIIPPEEYDPNGTNMYRLEKTFESIDAEKFVEDAMKTVGKLVGGSKGEDVAETATKLATEAVKQGLNIISESGGDRKGRSTLGELIESVVKEVMGDDRKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TVY8</accession>
    <name>Q8TVY8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02463">
        <property type="protein sequence ID" value="AAM02463"/>
        <property type="gene ID" value="MK1250"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1250"/>
    <dbReference type="HOGENOM" id="CLU_1514625_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="177" mass="18749" checksum="F755EF73258B0F02" modified="2002-06-01" version="1">MRVVLAAVLGLLAVLSPAAAAGTLDVKDVQYPAEVNACQEMEVKFKVVDASTGEPVSDASVIVRIAGEEHKDTPPENWERGHYAKVTSDSEGNVTAKLTAPGKEGTYYVWVKVEKDGYETLVKNLGELKVTGSSTCPNEGEQHRWGEQTGGMADVPVSPLVILGALCVLALAGATRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW42</accession>
    <name>Q8TW42_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">purE</name>
        <name evidence="7" type="ordered locus">MK1195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1195"/>
    <dbReference type="EnsemblBacteria" id="AAM02408">
        <property type="protein sequence ID" value="AAM02408"/>
        <property type="gene ID" value="MK1195"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1195"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1297"/>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_2"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="2"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AAM02408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="161" mass="17613" checksum="F26FA8DEBE19A461" modified="2002-06-01" version="1">MVDVLIVLGSRSDRDVAEKAAKVLDRAGVDYNVRVASAHRTPERIDELIEEYEPDVKVYIAIAGLAAHLPGVIAAKTLKPVIAVPVEAKLCGLDALLSTVQMPPGVPVAVVGVDRGENAALLALQILALEDEDVRSFLEEYREEMKKQVARDDDTIKERFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW70</accession>
    <name>Q8TW70_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1166"/>
    <dbReference type="EnsemblBacteria" id="AAM02379">
        <property type="protein sequence ID" value="AAM02379"/>
        <property type="gene ID" value="MK1166"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1166"/>
    <dbReference type="HOGENOM" id="CLU_052774_3_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="30"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="FIST_C" evidence="2">
        <location>
            <begin position="254"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="413" mass="44563" checksum="883033EF5A527025" modified="2002-06-01" version="1">MAAPLKAGVGVSKSSPEEALEGAADWGLDRIDAVLCAFSPRHDPHEVREAIVEYLEDDRCLILGLSSAGNITTDGFSDGSVAILAMELSKLVAMGMAIGTGLSEDPYRVARETVTEAAKSVEVDVSASLAPVATKLVTGNVDVTRHSLVDALLFTDDLCCLNHPEALMEALRGVLDCLGTVPRIVGGMTADEYEFERTYIFDDSGVYEDALAVLILYSSVKRGHAVDHGFVPSSEPMIVETEGVDVIRMDGEPALDVYEEVVGEEIGMETLLKYPLGVEDPGPRPYHLIRTPFEVDEEERTLKLVAQLPSGVAVRVMEPGDVEDSFKRAVQRALEDAGSPDELGAVLVFNCMARHLIVDTDDAVDLLRDLVSEDVPIFGFNCYGEYGLTPSGKFVQHNQTVVTYVLGSEVVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TW74</accession>
    <name>Q8TW74_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein specific for M.kandleri, MK-17 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02375">
        <property type="protein sequence ID" value="AAM02375"/>
        <property type="gene ID" value="MK1162"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1162"/>
    <dbReference type="HOGENOM" id="CLU_1192632_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="232" mass="24731" checksum="DAFD19564D7ACB7D" modified="2002-06-01" version="1">MIPGWYVGAVLVPQLVVTVVIAHGFSRKRRWERLLRRASREEVRRSLERTPGGGTVRLAFVPELLWCVPGRVLVLGSTGWLMLAVLYEFELLGLTRIVPEAKVRGLLAAVLALVYGTCAVAVLPGTPRPALPRLLAPAAVGGALGAVAVLKSLPPWLGSLAPVAMLVGLSSVIGYRGRLSASGGTAWDEVVEAVRYYPVSALSVGEREAEAAEKCALWFLACRVVEGTRSVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWA1</accession>
    <name>Q8TWA1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1135"/>
    <dbReference type="EnsemblBacteria" id="AAM02348">
        <property type="protein sequence ID" value="AAM02348"/>
        <property type="gene ID" value="MK1135"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1135"/>
    <dbReference type="HOGENOM" id="CLU_040020_1_0_2"/>
    <dbReference type="OMA" id="FGFHRMM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="370" mass="37402" checksum="A89417970E8C3916" modified="2002-06-01" version="1">MPGRVPKDVWLLGAVSLLNDVSSEGIFALLPYYLTALGGGPALVGGTWGGMELVKSLLNVVSGRVFGRPGWAKPAVAAGYGFSACMKLLLALVRDPVTAGLVASLERVGKGIRTPPRDAILAGLAGSEPGLVFGVHRALDTSGAVLGALLAGVLLTYGVPAATAVLAFALVGFLSLVPLIPVEERLEEGSSEGEGRGRFPRALAIVGLYRLGAVGWMMYSLRVLGVQGPAAAAFAYAGFSTLHALTSVPAGRLSDRFGPGVALCLGYAMTAATALLAGSGLAVAAFMLYGLAYGVVDAVERAAVAELSGTAASYGAYHALVGVGSLVCGLVVGWLWESVSPWAAFGYSACLTGVAAALAPVLILRRGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD8</accession>
    <name>Q8TWD8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-45 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02310">
        <property type="protein sequence ID" value="AAM02310"/>
        <property type="gene ID" value="MK1097"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1097"/>
    <dbReference type="HOGENOM" id="CLU_2299353_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="100" mass="11484" checksum="12D27C1496CD748B" modified="2002-06-01" version="1">MAGRPSIRYRYGIAEIPGDPKLAYETMWAALQGEARRIFDGLSRRLRRDPSSFHRSVERLWGEVRRRAQVSPALWELARELGLPEGTNLLNLLGRLLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWD9</accession>
    <name>Q8TWD9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-7 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02309">
        <property type="protein sequence ID" value="AAM02309"/>
        <property type="gene ID" value="MK1096"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1096"/>
    <dbReference type="HOGENOM" id="CLU_741048_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="373" mass="41660" checksum="070EA03D1292F915" modified="2002-06-01" version="1">MKWWEVPVRPKPSVLEKLHAAVSEPCEVLENFELFRRAMISTLDPDKPLVRALRGEEIHHVHPVRFLLEEVLDDPATLVDAVERITRWSLSGVFELRVALEEVTETPDVALGYLLEIRRAFLSAVPKYLEEPCLETVAGPLLVEVALMACTEGFLEDEDLGKAAELVAKAVDTDQCIVEALPSLEIQYGDIVPKFASPARVWTIFTLLCHTCANRLPPLEELAEPLENVELGPVEELELLNGYLEEAIAALEIEDERVERWISDILVDTVERAAELIQLLSQPTELAENILNSGATVRVGWIEIEPKEYDPLWEEKGAQESLYSRIKRIQNLIGVEHADEVIERASALGGVELPVGGGVEVESSVETLRRMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE0</accession>
    <name>Q8TWE0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK1095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02308">
        <property type="protein sequence ID" value="AAM02308"/>
        <property type="gene ID" value="MK1095"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1095"/>
    <dbReference type="HOGENOM" id="CLU_907970_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="306" mass="33554" checksum="A295AEA8CBFBC4B8" modified="2002-06-01" version="1">MEFSYAAMVAVLAAARGFAPVFVEHLPCESCRGKNCEIIRSSRCLMVATTELLCDVNVGAHVFFSDDLLVYRGVGDPESGISIHDVHDAGPDAARRILDRAGDALNRGLMFIATWDARHGPPEEFERHAPEALNLDVTGLTLPIPRIDTGLHPTDFRVVSAAEVRPEDVADFSDLDLIVDGRRYVVDPVLTAVAEVLGERAVEGLGAIRLEAPQGETLEWWMDSVKRYWKQLRVRSALRNGPSELAAAFWISILEVGTRLASDGWSVDRDTVEDAMRKSARMTEALVPKAIRSLLLGQLAVLVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE5</accession>
    <name>Q8TWE5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1090"/>
    <dbReference type="EnsemblBacteria" id="AAM02303">
        <property type="protein sequence ID" value="AAM02303"/>
        <property type="gene ID" value="MK1090"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1090"/>
    <dbReference type="HOGENOM" id="CLU_076812_2_0_2"/>
    <dbReference type="OMA" id="QDSRIKN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="143"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="216"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="274" mass="30117" checksum="DD3285DFB06A9801" modified="2002-06-01" version="1">MPELPSVEDLMSRNPVTVDADQSLKFALKTMRKRKVNRLPVTERVSEDRKELVGILTVLDAALAVADAMFGDRSPSRIKVSEVMSSPVITISPGATVLDAAQTMLVHGVSGLPVLDGDRLVGMITKTDLLELVRSEDYVALHMVKDPITVSAGTSLLHARRLMFEENAKVLPVVERERLVGLLTDRTLALELARLREKSPKGKFRSALKRARVDDVMRTPISVRTDYGLVDAAELIVRKRVPGVPVVNYQDEVVGVITKTDLLHLLVEELEAAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWE6</accession>
    <name>ALBA2_METKA</name>
    <protein>
        <recommendedName>
            <fullName>DNA/RNA-binding protein Alba 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">albA2</name>
        <name type="ordered locus">MK1089</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWE6"/>
    <dbReference type="SMR" id="Q8TWE6"/>
    <dbReference type="STRING" id="190192.MK1089"/>
    <dbReference type="EnsemblBacteria" id="AAM02302">
        <property type="protein sequence ID" value="AAM02302"/>
        <property type="gene ID" value="MK1089"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1089"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1144"/>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="OMA" id="ITRHKFI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="SSF82704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00285">
        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000151700" description="DNA/RNA-binding protein Alba 2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10142" checksum="E11D650FD5F63169" modified="2002-06-01" version="1">MAEENVIYVGNKPVTNYVLAVMTQFSEGADEVKLVARGRAISRAVDVAEFIRNNVMPEVEVKDIEIGTEEIETEEGDTISVSTIAITLAKPSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG4</accession>
    <name>OTC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="2">OTCase</shortName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argF</name>
        <name type="ordered locus">MK1070</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks the conserved leucine residue in position 272, which is part of the ornithine binding site; it is replaced by a methionine residue.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWG4"/>
    <dbReference type="SMR" id="Q8TWG4"/>
    <dbReference type="STRING" id="190192.MK1070"/>
    <dbReference type="PRIDE" id="Q8TWG4"/>
    <dbReference type="EnsemblBacteria" id="AAM02283">
        <property type="protein sequence ID" value="AAM02283"/>
        <property type="gene ID" value="MK1070"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1070"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1124"/>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113066" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="312" mass="35494" checksum="85CBF8FB85E8351F" modified="2002-06-01" version="1">MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPSTRTRVSFEVAMHQLGGQAFTYTKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEFARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVAFVGDGNNVCSSLALVCATLGWDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEAEREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRLHVQKAILALLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWG8</accession>
    <name>Q8TWG8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02279">
        <property type="protein sequence ID" value="AAM02279"/>
        <property type="gene ID" value="MK1066"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1066"/>
    <dbReference type="HOGENOM" id="CLU_2127839_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="113" mass="12379" checksum="3C7EEE695AE22DF0" modified="2002-06-01" version="1">MGLAGYLPVELCRFVRNPDPNTSGKRAEQLVRRYGPYLGVLTVMVLFGSYITLATMAVAGLGGDRRAHLSALAGSMLLALLGYGATHTTLRALQPWLMGQEWGEELLRRFGIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWH4</accession>
    <name>KAD6_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MK1060</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWH4"/>
    <dbReference type="SMR" id="Q8TWH4"/>
    <dbReference type="STRING" id="190192.MK1060"/>
    <dbReference type="EnsemblBacteria" id="AAM02273">
        <property type="protein sequence ID" value="AAM02273"/>
        <property type="gene ID" value="MK1060"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1060"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1114"/>
    <dbReference type="HOGENOM" id="CLU_079096_0_1_2"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="PTHR12595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153907" description="Putative adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <sequence length="190" mass="21695" checksum="942DD7EB07F64B2F" modified="2002-06-01" version="1">MKIAITGTPGVGKTTVCEALRDLGFDVVHLNKVAREMDAILEEDEQRQAKVVDLHALRRYVEEWEPESDPAFVESHYAHLMPTDLVIVLRLHPSELERRLKEKGYPPEKIAENLEAEFVGVCYGEAVEVRSEGCFIRPPEDVIQVNVTGLSRAEAADRVLEAVNHRRGDDVDWLSDEEAQRTVERYLKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI6</accession>
    <name>Q8TWI6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-methylhydantoinase B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hyuB</name>
        <name evidence="3" type="ordered locus">MK1048</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1048"/>
    <dbReference type="EnsemblBacteria" id="AAM02261">
        <property type="protein sequence ID" value="AAM02261"/>
        <property type="gene ID" value="MK1048"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1048"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1101"/>
    <dbReference type="HOGENOM" id="CLU_020413_1_0_2"/>
    <dbReference type="OMA" id="QVGMTNT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_B" evidence="2">
        <location>
            <begin position="3"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="513" mass="55709" checksum="C6322EC07F33E7DF" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWI7</accession>
    <name>Q8TWI7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylmuramoylalanine-D-glutamate ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">murD_2</name>
        <name evidence="3" type="ordered locus">MK1047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1047"/>
    <dbReference type="EnsemblBacteria" id="AAM02260">
        <property type="protein sequence ID" value="AAM02260"/>
        <property type="gene ID" value="MK1047"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1047"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1100"/>
    <dbReference type="HOGENOM" id="CLU_560971_0_0_2"/>
    <dbReference type="OMA" id="FKMMSGI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="2">
        <location>
            <begin position="115"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="1">
        <location>
            <begin position="310"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="470" mass="50850" checksum="B1CFCAA3795BD302" modified="2002-06-01" version="1">MKRVIVVGAGSAGRSVARLLNHVGYDVVINDIRDWEDFTTEEREYLEVLEREGVEVALGGHDRELFESADAAFVSPAIPEDAEGRKLAELVPEEITVEDVAGLLGDLFPVPAIGITGTNGKTTTTWAVAHLLEACGYEVWRCSSLDRHLVIEAIVDGVVTGEIEGYDVAVVELPHGTIRLAAGIELDVGVLTNVSPEHLDEFGGSFERYVRRKLTITEMSEVLVAGYDCEELRKRLNDAVWYSVRDPDADYYGRRENGRLVVKGPDASVEADFHLIGYYVENCTGAVAACLEFGADPEGVAEGLATFEGVPGRMELLGEFSGRVAYIDAAHNPDGLKASLPPFRELADERGGRLLVSVDNPDTVTERDKFEFGRIVGEYADFVAASGYNETLERLDRSAAEEVVKGAESAGCEGVAVDSVREAAEEVAARSEEGDVLLHVGPGVVNAYDRVKRAFLRGLTSVLGEPSDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ4</accession>
    <name>Q8TWJ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe-S oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1040"/>
    <dbReference type="EnsemblBacteria" id="AAM02253">
        <property type="protein sequence ID" value="AAM02253"/>
        <property type="gene ID" value="MK1040"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1040"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1093"/>
    <dbReference type="HOGENOM" id="CLU_1411440_0_0_2"/>
    <dbReference type="OMA" id="HTPGKYL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="174"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="546" mass="60627" checksum="8E6ABB928FDFF580" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ7</accession>
    <name>Q8TWJ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Precorrin-6B methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cobL_1</name>
        <name evidence="2" type="ordered locus">MK1037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK1037"/>
    <dbReference type="EnsemblBacteria" id="AAM02250">
        <property type="protein sequence ID" value="AAM02250"/>
        <property type="gene ID" value="MK1037"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1037"/>
    <dbReference type="HOGENOM" id="CLU_1438119_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="20623" checksum="DD68E4BEFFDB1CF9" modified="2002-06-01" version="1">MFNVVDPSDLVTEGVPGPTKPVMKATVLAVLRPRPGERILEIGAGSGSLTLELARAVGPLGRVYAVEGDKEAFRSLERNVRDFCLEDRIEIVRGWAPEALEDVDEVDAAVVSGSERLEEVLLALAERVRRAILLNAVTPETFATAVKALDGWRRSCLCMVWGEGKVLRRGTLFSGMRTSYLALFEPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWJ8</accession>
    <name>Q8TWJ8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-38 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02249">
        <property type="protein sequence ID" value="AAM02249"/>
        <property type="gene ID" value="MK1036"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1036"/>
    <dbReference type="HOGENOM" id="CLU_870478_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="33968" checksum="0085D165D909C949" modified="2002-06-01" version="1">MPALVLIIAVSIAMTPAHGRVGGFVSGHYYLDPEFDAPNTSFGSDIDGDGRVDRIWCAEACAVDMYNLGVAMGWWNGDPVTPLQYEISATGEHDDDNLDIIDAFQAIAGSDAASKVFEVAVVSLLNRPSVYHAVLVLGAVQEGSRRYFVVDDESTSDVGELLLVPYDLFLSNLHPGILEFFGPVEVVNRLYSVLSGAISPLTVIVSSKRGRTLTVVLLTDLPGDSLAAFTEELWKAALEGYPVAVAVSKPFLDLGGVTRIRCDAPVTGDVETRRPLNGVESGEGPSPPLGIPIPFIGTVGIAGLFFLLLTFLGLSYLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWK4</accession>
    <name>Q8TWK4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK1029</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02242">
        <property type="protein sequence ID" value="AAM02242"/>
        <property type="gene ID" value="MK1029"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1029"/>
    <dbReference type="HOGENOM" id="CLU_1335057_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="205" mass="22552" checksum="6FF2083F4C138002" modified="2002-06-01" version="1">MKRGLKIAAYVAGGLLLCTVSANALLGMREPPRFAEIDNPSLGDRVFLAISGLVVGIMGVSRGIWELRNVERVRAALGLLKKAERYLEEGSERIPAKYNAVTYALRALRCVKNAVEILEEMEEELESQERELKEKAKEKEKAEKEGEEEKVEKRGSSDVRTAERRDGAGDENSDDRRSRGDGTADRGRREEDRGVSDGGGSEGSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-09-21" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM4</accession>
    <name>SECD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secD</name>
        <name type="ordered locus">MK1009</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWM4"/>
    <dbReference type="SMR" id="Q8TWM4"/>
    <dbReference type="STRING" id="190192.MK1009"/>
    <dbReference type="EnsemblBacteria" id="AAM02222">
        <property type="protein sequence ID" value="AAM02222"/>
        <property type="gene ID" value="MK1009"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1009"/>
    <dbReference type="HOGENOM" id="CLU_007894_5_1_2"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000412687" description="Protein-export membrane protein SecD">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <sequence length="403" mass="43385" checksum="44534461B3FDFC46" modified="2002-06-01" version="1">MSGLGWMKENWRLLVITAVWIVAATSLAVKGVNLGLELKGGTMVIAKTDHPVSKKEMDQTVTVLESRLSTFGFKGIKIQPVGRDHIIVMLPGTPPKEAVELITKPGRFEAKYKGKTVITGQDIESVESPRIERVEGGYQWSVPFRLTAEGARKFAEVAKNAPGQPIDMYLDNKKVSSPRISEDLAMAAASGHMEREIEIVGGAKTKEQAEREAKEIMAVLRSGQLPAKLVPEGVYSVSATLGQNFLKMAMIAGAIAFAAVSVIIALRYRDIRISGPILFTGSSEVVFLIGLASLTGFTIDLPALAGIILSIGSGVDDLIVITDEIVRGERRKEEVTLRQRIKRAFSVVLASFATLAAAMAVLFVAGMGLLKGFAIMTIAGAFYGVVITRPVYADLLKKILGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWM8</accession>
    <name>Q8TWM8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK1004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02217">
        <property type="protein sequence ID" value="AAM02217"/>
        <property type="gene ID" value="MK1004"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK1004"/>
    <dbReference type="HOGENOM" id="CLU_2857105_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="64" mass="6659" checksum="3C10F4181A184926" modified="2002-06-01" version="1">MVVGQKILASAVAHLLPFLVGAVGIYFILTASMKDDEHLRRLGFLIIVIGGPTSVLVSIAIVGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWP2</accession>
    <name>RS19_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps19</name>
        <name type="ordered locus">MK0990</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TWP2"/>
    <dbReference type="SMR" id="Q8TWP2"/>
    <dbReference type="STRING" id="190192.MK0990"/>
    <dbReference type="EnsemblBacteria" id="AAM02203">
        <property type="protein sequence ID" value="AAM02203"/>
        <property type="gene ID" value="MK0990"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0990"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.1037"/>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_2"/>
    <dbReference type="OMA" id="VIRTHCR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_S19A/S15e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130003" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="149" mass="17655" checksum="4DB189DB03D159D5" modified="2002-06-01" version="1">MAEERSKDDIPDWWEKEWVEFRYRGYTLGELMKMPIEEFIELLPARQRRSLKRGLPSRHKKLLRKVRRARRLLRRGKKPPVIRTHCRDMIILPEMVGLTIAVYNGKEFKEVKIEPEMIGHYLGEFAKTRKTVEHGGIGATRSSLFVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWU4</accession>
    <name>Q8TWU4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein specific for M.kandleri, MK-21 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0937</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02150">
        <property type="protein sequence ID" value="AAM02150"/>
        <property type="gene ID" value="MK0937"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0937"/>
    <dbReference type="HOGENOM" id="CLU_648318_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="423" mass="48357" checksum="2FCA3B824287FDC7" modified="2002-06-01" version="1">MEEADNPVLNLVACLSRAFDSLDSAEIKVPEGCRVVWHGEHLLILSKDESMEVRLNVPENVTISPRVAEGPMEIRVHVEREEEPERGLEDRLLSAVREFRRRLRELSVSTSRREEAPSEVAIPPIFEVDSPTLLELEYSMGRISYVFGRTELKRALFALALAKTATVCAARNAVSSPTNLVEEVEADTVVSPYLRVGREIVELDAIPANRVISLKKIPEDVKVWWLNWKYLPRVMAWGVDGITAEIGWRSSDLAVGYVDIQSSLYAHFLSALLNVPLFDFSYMWFLQRRTKRSKRKLWKDEKGECHYVFWTQMEAFEKLGVEDVILLCTREECEGWVTECVREDTDVRVVEVFEEDTRRMGLRVEELALTLGEEIHDRHPVTPEHPRIPSQLGSAPELPVSLDTYLTVAKMAEERILPIISSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY7</accession>
    <name>Q8TWY7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted DNA-modification methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0894"/>
    <dbReference type="EnsemblBacteria" id="AAM02107">
        <property type="protein sequence ID" value="AAM02107"/>
        <property type="gene ID" value="MK0894"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0894"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.936"/>
    <dbReference type="HOGENOM" id="CLU_057819_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0020" evidence="1">
        <location>
            <begin position="94"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="268" mass="29971" checksum="61B090F0D9853367" modified="2002-06-01" version="1">MFAFLLAGDNVELALMELKGALRAEVGEIEVEVHDRVAVVEGLTDEEAERVVRRLARTHAVYRVGEGRVRVEIGGERRPVRLLAEREKLSDRKPHKRPEFMPDSTEPFLARVLVNLAEARRGERLLDPMCNVGGILIEAGLIGCVPVGVEVREELVERTERNLRHYRIEEYELHTGRAEEVDEILDEPVQAAAVDPPYGRSSYIEGGPVERLLLNTLDALSEVVEGKVSLTAPVDAWEEVKESVKRVEGEVRDRVHGSLTRVIAVVVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWY9</accession>
    <name>Q8TWY9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis translation initiation factor eIF2B subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MK0892</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0892"/>
    <dbReference type="EnsemblBacteria" id="AAM02105">
        <property type="protein sequence ID" value="AAM02105"/>
        <property type="gene ID" value="MK0892"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0892"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.934"/>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTP_transferase" evidence="3">
        <location>
            <begin position="3"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM02105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="425" mass="46543" checksum="2C21B47B7E97B870" modified="2002-06-01" version="1">MIGIVLAAGEGTRMRPLTKTRPKVLLPVADRRLIDFSIEAMKRIGVEHLVVVVEYLAEKVERYVKDRWGDSFELEFVRQGKPLGTAHAVYVAWREIEPDETVVITNGDLVFDSELLERAVREHEGVASMVLVEVEDPSEFGVARLQDGYVVELVEKPKPEEAPSNLANAGVYVAEPEFERFLERVKPSPRGEFEITDALLDAAIDEGVLGISYDGFWSDVGRPWDLLDANAWALRNAMSRPEVEGVIEENVELRGPVWVAEDAILRSGAVVEGPAYIGPGCEIGPNCYIRPATTLVRDVRIGQAVEIKNSIIMEGTNVSHLSYVGDSVIGAKCNLGAGTIIANLRHDERNVKVVVKGELEDTGRRKFGAVLGDGVKTGINTSILPGRKLGPYSATAPSTVVRKNVPEGKMLVQGDQILVDWEGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TWZ4</accession>
    <name>Q8TWZ4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0887</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM02100">
        <property type="protein sequence ID" value="AAM02100"/>
        <property type="gene ID" value="MK0887"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0887"/>
    <dbReference type="HOGENOM" id="CLU_1092435_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM02100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="254" mass="28423" checksum="12DCF47855BD7598" modified="2002-06-01" version="1">MLKVTVCPPCYGFREVCHKLAQDGEIVDVSDARRLSDITSLCDEGADRVVIAHMDAPTVLEWLERRDRYALRSIRSKVYNVLSALLNRFKEVHVVVYSQVFLDFVPDSIVREVSVELVKVEGSAKDILTWPPYGPCPEALKRGGTLEEMNEHVSELVEETGSTILGEIARRDVRVVRTMYLSATEILGRICRNFREFKLGTKMLDALEAAGVLTSRNVGYRMFSWSPAAVLGRRSELVNGVLNAVLEMEGAVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX10</accession>
    <name>Q8TX10_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenylate cyclase, class 2 (Thermophilic)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cyaB</name>
        <name evidence="2" type="ordered locus">MK0871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0871"/>
    <dbReference type="EnsemblBacteria" id="AAM02084">
        <property type="protein sequence ID" value="AAM02084"/>
        <property type="gene ID" value="MK0871"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0871"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.913"/>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="OMA" id="QHPCRDF"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="PTHR21028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="181" mass="21035" checksum="4E0F5C257D5B513F" modified="2002-06-01" version="1">MYEVEVKVKLKNPEDVRKRLEELGEKVRTVRQRDLYYQHPCRDFAETDEALRLRCSDDKVALTYKGPKVGREKSRVELEVVVDDFETTDAILRHLGFEPLEHAEVKKLRTVYTLEVNGEKVVAALDEVEGLGTFLELECKADDESEVDEKEKLLVSILEELRVEGKRVRHSYLEMLLDQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX29</accession>
    <name>Q8TX29_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrolipoamide dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lpd</name>
        <name evidence="2" type="ordered locus">MK0849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0849"/>
    <dbReference type="EnsemblBacteria" id="AAM02062">
        <property type="protein sequence ID" value="AAM02062"/>
        <property type="gene ID" value="MK0849"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0849"/>
    <dbReference type="HOGENOM" id="CLU_016755_0_2_2"/>
    <dbReference type="OMA" id="HMVGDRM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="434" mass="46790" checksum="BE85A6DCAB065762" modified="2002-06-01" version="1">MDVVIGAGPAGRTYAMILAEAGHEVLLLDRNGKEGTGGKCLNEACVVLGALIEAARLVVWAKLGIPGVELDVGDINFRRLTRSVRKVVETIRQRLIKETERAGVEILRAEAVKVDESLNVYTKDGDVLEADRVLIATGSRPAIPEVEGVDSDAVFTFREILEMEVPSELCVVGGGPTALESAFAFAALGSEVVLAYRSRILPNAPEEVRREILKDLELVGVNAVRAGELRAIRETSSGVECRFERGATVADAVLLATGLEPNSDIAANSGLPLRKDGSVVVDDGMRTPRDGVYAAGDVTGPPYLTPVARYEGTVAALNALGKNVRRGNPPAPRVIRLFRDFGRLELRGIDWEGSLPTPVGGPAFWMLHHGIKGKMVCRKRGVTTEAFIVAPRIAPMLPYPRCVEPDWRLIEVHPTPDPVIGLLKQLGLRRTLGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-08-16" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX47</accession>
    <name>ADEC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine deaminase</fullName>
            <shortName evidence="1">Adenase</shortName>
            <shortName evidence="1">Adenine aminase</shortName>
            <ecNumber evidence="1">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ade</name>
        <name type="synonym">adeC</name>
        <name type="ordered locus">MK0829</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenine + H(+) + H2O = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TX47"/>
    <dbReference type="SMR" id="Q8TX47"/>
    <dbReference type="STRING" id="190192.MK0829"/>
    <dbReference type="PRIDE" id="Q8TX47"/>
    <dbReference type="EnsemblBacteria" id="AAM02042">
        <property type="protein sequence ID" value="AAM02042"/>
        <property type="gene ID" value="MK0829"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0829"/>
    <dbReference type="HOGENOM" id="CLU_027935_0_0_2"/>
    <dbReference type="OMA" id="TDHECFT"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01295">
        <property type="entry name" value="AdeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="PTHR11113:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142441" description="Adenine deaminase">
        <location>
            <begin position="1"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="599" mass="65401" checksum="2978B26973FFB877" modified="2002-06-01" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX77</accession>
    <name>Q8TX77_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prephenate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tyrA_2</name>
        <name evidence="2" type="ordered locus">MK0798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0798"/>
    <dbReference type="EnsemblBacteria" id="AAM02012">
        <property type="protein sequence ID" value="AAM02012"/>
        <property type="gene ID" value="MK0798"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0798"/>
    <dbReference type="HOGENOM" id="CLU_036672_1_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM02012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="420" mass="46754" checksum="D7043CF19B24C201" modified="2002-06-01" version="1">MRIAILGGTGAMGRLIARELRDDGHEVVITGSNPHTAERVARELDVEAAPTNVDAAKDADVVVVSVPISVTEDVIREVAPHVPEGSLLTDVTSVKVRPVRAMLEHAPEDVYVLGTHPLFGPTVPSLRGQTVILTPTERSGPWTRRVRRYLERKGARVVETTPEEHDRTMAVVQCLTHAVLLAAGAAIGRFLPSLELDIEEVASPVYRLLMDVVGRIAGQDPRLYAEIQAFNPYGDEAREELLRALRRFHEHAHDHNALTEYIAESRERLGRELDLEACQRRTDKLLSYLADELRILQEGIEAILLDVYTNEALEGEVTGDRDTIRLDGRELPRDRYKILPRCPKELAHFDGEVTVLELRTGADPGDVAAVVDALGRNLRALRAERRGERVRVEVEYTGKESLEGLLRKLRALGLEVRVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TX80</accession>
    <name>Q8TX80_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Selenophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM02009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0795"/>
    <dbReference type="EnsemblBacteria" id="AAM02009">
        <property type="protein sequence ID" value="AAM02009"/>
        <property type="gene ID" value="MK0795"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0795"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.836"/>
    <dbReference type="HOGENOM" id="CLU_073250_0_0_2"/>
    <dbReference type="OMA" id="VPMVGGH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02192">
        <property type="entry name" value="PurM-like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017668">
        <property type="entry name" value="Methan_mark_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011413">
        <property type="entry name" value="UCP036540_AIR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036540">
        <property type="entry name" value="UCP036540_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03267">
        <property type="entry name" value="methan_mark_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="1">
        <location>
            <begin position="48"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="2">
        <location>
            <begin position="192"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM02009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="327" mass="35757" checksum="E34A3D3E11BAC015" modified="2002-06-01" version="1">MNLRELARELRSFEGVTRKHPVKTVVEGLLEPLDVTFEGEVIADVGEDAAAVKVGDDILLIAADGIWGKLIDRDPWWAGYCAVLVNVNDVLAMGGRPVGLVNVLSTSDVDVCREILEGMREGAWKFSTPVLGGHTHPDTPYTAVDAAIVGVTDEEHLVLSSTAEEGDLIVFVIDLNGRPYPEYPLNWDTTTMKDPDYLRRQMEAVVEASKLVKAGKDVSNPGLVGTLAMMLEASGCLGAEVWLDSIPKPEDVDMVTWLKMYPGMGFVYAVDSERDVRAIRRVLSDARLEVSVIGEVTEDGVVKVSEGDESARVFDFREDRILGVSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXA0</accession>
    <name>Q8TXA0_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease J</fullName>
            <shortName evidence="8">RNase J</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnj</name>
        <name evidence="10" type="ordered locus">MK0775</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0775"/>
    <dbReference type="EnsemblBacteria" id="AAM01989">
        <property type="protein sequence ID" value="AAM01989"/>
        <property type="gene ID" value="MK0775"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0775"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.816"/>
    <dbReference type="HOGENOM" id="CLU_008727_4_1_2"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001587">
        <property type="entry name" value="RNase_J_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01292">
        <property type="entry name" value="UPF0036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Lactamase_B" evidence="9">
        <location>
            <begin position="19"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="8">
        <location>
            <begin position="390"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AAM01989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="453" mass="50760" checksum="099898A0C9CC54CA" modified="2002-06-01" version="1">MEPLTVEVFAVGGYGEAGGRNMTAVRVDEEIVIFDCGMSLDKSLVFEKDFQKASARELRRVKAIPNDPILRPHRSKTVAAVLSHAHLDHIGAVPKLLFKYKCPVFGTEFTIELVKADLRNEIRYADEAEDIMINLYTVEPGDEVQISSKLRLEFIPISHSIPCCVLPVLHTPYGAIVYACDFKFDDNQIIGYKPDYKRLKQLGKEGVLLLITESLRVAEEIKTPSESVAREMVNDVLRFADEESEGIIVTTFSSHIERIQAIADTADRLGRKVILAGRSMGKYGRIAEELGLLNLPAGARIYDRPETIRRGLERANKEKEDYLLIVTGHQGEPGAVLPRIVDGELPYRLTEEDSVVFSSSTIPSPINRANRYVLDTKLRLKGVKMFKDVHVSGHAGREDHRLMLRMLQPEFIVPAHGDPDMLAAYAELATQEGYEVNRDVFIMFDGTKLSLPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXF3</accession>
    <name>Q8TXF3_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CO dehydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cooC_1</name>
        <name evidence="2" type="ordered locus">MK0721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0721"/>
    <dbReference type="EnsemblBacteria" id="AAM01935">
        <property type="protein sequence ID" value="AAM01935"/>
        <property type="gene ID" value="MK0721"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0721"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.762"/>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="OMA" id="GCYCAVN"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="3"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="257" mass="28667" checksum="B250C2B9E85D3B97" modified="2002-06-01" version="1">MIIAFTGKGGTGKTLLAALTVLELLDRHPDADLLVVDADPDANMPDVLGVEVDTTLGEVREHFKREIEGGELPPGFDKQAYMEYLVMTALQESDDYDLLVMGRSEGKGCYCAVNHWLRRVMRELLPNYDYVVMDCEAGLEHISRGIIEGVDTVLTVVDHSYKALRTAVRISRLIDELESDVGEMWVVANRVTEGEYAVIREKGEELGLRFAGFVRPDDEVVRLELHGRPLTELPPDAKVRRDMRAVLTRVGVLLSDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-03-25" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXG3</accession>
    <name>HISX_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histidinol dehydrogenase</fullName>
            <shortName evidence="1">HDH</shortName>
            <ecNumber evidence="1">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisD</name>
        <name type="ordered locus">MK0711</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXG3"/>
    <dbReference type="SMR" id="Q8TXG3"/>
    <dbReference type="STRING" id="190192.MK0711"/>
    <dbReference type="EnsemblBacteria" id="AAM01925">
        <property type="protein sequence ID" value="AAM01925"/>
        <property type="gene ID" value="MK0711"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0711"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.752"/>
    <dbReference type="HOGENOM" id="CLU_006732_3_3_2"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135895" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <sequence length="431" mass="47335" checksum="50DD46829C9EB5DC" modified="2002-06-01" version="1">MRLLVRPEEEELERVLRRSEMDVTEVLPDVERIFEDVVERGDEALLEYTERFDGVKLEAEDLRVSEDDFEVARELVDERTVEALEEAAHRIEEFHRKTLPRVDRITFDVEGTECGLTLRPIPRVGCYVPGGRAAYPSTALMTVIPARVAGCREVVVCTPPADNDVRASPEVLVAVEIAGADAVYRVGGAQAIAALAAGTETVLRVDKIVGPGNVYVTAAKLLAYSRGLTDVDMPAGPSEVFVIADDSANPDWLARDLIAQAEHDPHAAAVLATDSEEIARAVKERVEELLDAGIEREEIVLKALDRNGWIVVLDSLEECVRLANRYAPEHLQLCVENPEELLQDVENAGAVFVGHLTAVPFGDYATGPNHVLPTGGFARARGALGTWDFVKIIPIQRLREGDVERLAPIVEELAEREGLPNHAEAVRARRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXH8</accession>
    <name>Q8TXH8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01910">
        <property type="protein sequence ID" value="AAM01910"/>
        <property type="gene ID" value="MK0696"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0696"/>
    <dbReference type="HOGENOM" id="CLU_1745566_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="149" mass="15728" checksum="2C0FC772CCE8CDE4" modified="2002-06-01" version="1">MFMSFSRGQASLEYLLVLLIGVTAVGMAMAFVKEGMYGSGGVEEAASALEAKAMAEQLARVLDKAAACDVGTRFTVRIKGRPGTQGSYTVSVEPTGQHDEYYVIVEGNGVKGVAKFYSFGKDVVVENVTVPGVVEVSVVEGPKLVLRNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-07-26" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXJ0</accession>
    <name>RL30E_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30e</name>
        <name type="synonym">rpl30</name>
        <name type="ordered locus">MK0683</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXJ0"/>
    <dbReference type="SMR" id="Q8TXJ0"/>
    <dbReference type="STRING" id="190192.MK0683"/>
    <dbReference type="EnsemblBacteria" id="AAM01898">
        <property type="protein sequence ID" value="AAM01898"/>
        <property type="gene ID" value="MK0683"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0683"/>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="OMA" id="RPHKIMA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="L30/L30e/YlxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_L30e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_L30e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="PTHR11449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449:SF1">
        <property type="entry name" value="PTHR11449:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146148" description="50S ribosomal protein L30e">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <sequence length="109" mass="11732" checksum="DD05DD812157403F" modified="2002-06-01" version="1">MQEIERQIRMAVETGDVVLGSNQTIKLLKLGKPKLVIVAANCPAEIREDIEYYAELADVPVFVYPGTSMDLGDVCGRPHVVASMAVLDDGESDLIATVRKAMEEGAAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXK4</accession>
    <name>Q8TXK4_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0668</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01883">
        <property type="protein sequence ID" value="AAM01883"/>
        <property type="gene ID" value="MK0668"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0668"/>
    <dbReference type="HOGENOM" id="CLU_2257383_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="103" mass="11806" checksum="B01F13175E37B67D" modified="2002-06-01" version="1">MLTVTDIVEKIAEILERGDGRYLIMGMVQAHGLGLRSLADSVDVPVEVLATIEIEGFGKVPHKSLVRKLAEWIEERDLDPEDLVKAGWARFELEYFEPELWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXL6</accession>
    <name>Q8TXL6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01861">
        <property type="protein sequence ID" value="AAM01861"/>
        <property type="gene ID" value="MK0646"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0646"/>
    <dbReference type="HOGENOM" id="CLU_2695793_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="73" mass="8040" checksum="BD1D59D9EC771352" modified="2002-06-01" version="1">MSRNVLRNRGQGGVEYLLLAAAICVWAFLEISVLWWVMAKDAGRAAQEAAKMAAEMARQKGVEIVEKYANAIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-06-07" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXM1</accession>
    <name>RL40_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L40e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl40e</name>
        <name type="ordered locus">MK0641</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXM1"/>
    <dbReference type="SMR" id="Q8TXM1"/>
    <dbReference type="STRING" id="190192.MK0641"/>
    <dbReference type="EnsemblBacteria" id="AAM01856">
        <property type="protein sequence ID" value="AAM01856"/>
        <property type="gene ID" value="MK0641"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0641"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.680"/>
    <dbReference type="HOGENOM" id="CLU_205640_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_L40e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_L40e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="PTHR39649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000138782" description="50S ribosomal protein L40e">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <sequence length="49" mass="5524" checksum="772321FE0534961D" modified="2002-06-01" version="1">MAKFPEAEARIFNKLICMKCNARNPPDATKCRKCGYKGLRPKAREPRGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN1</accession>
    <name>AROC_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">MK0631</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXN1"/>
    <dbReference type="SMR" id="Q8TXN1"/>
    <dbReference type="STRING" id="190192.MK0631"/>
    <dbReference type="EnsemblBacteria" id="AAM01846">
        <property type="protein sequence ID" value="AAM01846"/>
        <property type="gene ID" value="MK0631"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0631"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.670"/>
    <dbReference type="HOGENOM" id="CLU_034547_0_2_2"/>
    <dbReference type="OMA" id="MLSINAV"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140691" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="305"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="386" mass="41328" checksum="7F04C8AA487E9051" modified="2002-06-01" version="1">MNTLGRLFRVTTWGESHGPALGAVVDGCPAGLPLSEDDVQRELDRRRPGQSGVSTPRSERDRVEILSGVHEGRTLGTPISMIVWNEDVDSSKYEPIRTRPRPGHADVTYRWKYGHVDYRGGGRASGRTTVGIVMGGAVAKKLLREAPSNDPLGIEIVGHVVRVGSVEADPGDLSAEEIMQYAESNPVRCADPDAAEEMLGEIERARENGDSVGGTIEVIAENVPPGLGDPVFGRLDGELAGALMNIPAVKAVEVGSGVRCSEMHGSEHNDPIWWDGHPVVDGDNSGGVLGGISHGGRLVVRVHVKPTPSVSVPQRTVDLESEEEVEIEVEGRHDPCICPRAVPVAESVVAIVLADAVLRAGYVNPDSVELPAASVEDRWRTLKRHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN5</accession>
    <name>VHUD_METKA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu iron-sulfur subunit D</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuD</name>
        <name type="ordered locus">MK0627</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0627"/>
    <dbReference type="KEGG" id="mka:MK0627"/>
    <dbReference type="HOGENOM" id="CLU_095272_2_0_2"/>
    <dbReference type="OMA" id="XGYGAAD"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000249590" description="F420-non-reducing hydrogenase vhu iron-sulfur subunit D">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="14445" checksum="6047EAA8B011A07A" modified="2008-02-26" version="3">MSEESVVVAFCCYEUGYGAADLAGTGRAQYPSSVRIVRVPCTGRVGIEHILTALAKGAWTVFVAGUKKGECSYEDGNLKCERRVQAAKKLLEELGIEPERVEIYFMSSAEADKFVAAVKEMHERAKELGPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXN6</accession>
    <name>Q8TXN6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein specific for M.kandleri, MK-7 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0626</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01841">
        <property type="protein sequence ID" value="AAM01841"/>
        <property type="gene ID" value="MK0626"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0626"/>
    <dbReference type="HOGENOM" id="CLU_914017_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="304" mass="33812" checksum="15CC84B674FDFF6C" modified="2002-06-01" version="1">MALWLDVPVAPTDSFYRKLFEAVWRILGDRARGIASERLPALRRCYTVSPDSSTFLSENVPGVRLLDEFRPGELLEEILSETVSGELGRRFWGSLVVSVVERGDVAGLEAMKRSIEALRAVDVTPWLDALALDVLVALAEDVGEDILGDSLQLVAERLDVDEDGVLRVSPLALEFAGYCFEMILLGYVVAYRKPEEIEVVSCDVFAVEDVRRDIERRISEVKSGDLELAAEVIEEVLAPYLRVVTDVRGYLEEARSMDPSIALEVDGFSVSIAPKELDPEWDRHVQEAIEKLTGSGGKARRVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXP7</accession>
    <name>Q8TXP7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Methionine aminopeptidase</fullName>
            <shortName evidence="6">MAP</shortName>
            <shortName evidence="6">MetAP</shortName>
            <ecNumber evidence="6 7">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">map</name>
        <name evidence="9" type="ordered locus">MK0613</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6 7">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6 7"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0613"/>
    <dbReference type="EnsemblBacteria" id="AAM01828">
        <property type="protein sequence ID" value="AAM01828"/>
        <property type="gene ID" value="MK0613"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0613"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.650"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
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        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
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        <property type="entry name" value="Pept_M24"/>
    </dbReference>
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        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
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        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
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        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00557">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="met_pdase_II"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="8">
        <location>
            <begin position="4"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="161"/>
        </location>
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        </ligand>
    </feature>
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        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="290" mass="32063" checksum="13FDA3CD6705A735" modified="2002-06-01" version="1">MDDEFQEAGKVVKRVLRRAAREIDIGDRLLEIAEYLEECVRDQGAEPAFPVNLSLNEVAAHYTPSPDDEVEVSPGDILKVDIGAHVNGAIGDAAITLSFDNDLGERLAEAAREALEAAIETIRPGVECREVGRAIGEVCRDRGFKPVIGLTGHQIEPWNLHAGVSIPNDDLPGYEDKLEEGMVLAVEPFVTTEDGEGDVKPGSTVEIFSVRNPNQRSRLNLHRIYEDRKSLPFARRWVGKAPRVKFELLRLSKLGAVKAYPELVEVNGEPVAQWEHTVVVTERGCRVLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXQ7</accession>
    <name>Q8TXQ7_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homolog of ABC-type nitrate/sulfonate/taurine/bicarbonate transport systems, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAM01818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAM01818"/>
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    </dbReference>
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    <dbReference type="PATRIC" id="fig|190192.8.peg.639"/>
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    <dbReference type="OMA" id="YSLFPWR"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_1"/>
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    </dbReference>
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        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="5"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="246" mass="27590" checksum="3D07C4A58A44E88C" modified="2002-06-01" version="1">MAVILKVEDLHKSYDDLKVLDGITFEVSEGEFFAIVGPSGCGKTTLLKIIAGLEDYDAGRVLVDGEEVREPGPDRALVFQEYAIFPWKTVLENVMFAPLMQGKDPEEAERIARECLKVVPGLEGFEDAYPKQLSGGMKQRVAIARALAAEPRILLMDEPFAAVDAQTRNKMQEELLKIWERTGQTILLVTHNVEEAVFLADRVMVLSPRPAEIVDIVEIDLPRPRDRTDPEFVELRARILDMIGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXR9</accession>
    <name>Q8TXR9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri with repeats, MK-6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0591</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01806">
        <property type="protein sequence ID" value="AAM01806"/>
        <property type="gene ID" value="MK0591"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0591"/>
    <dbReference type="HOGENOM" id="CLU_1052158_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="264" mass="29653" checksum="176F667E2A2C67BE" modified="2002-06-01" version="1">MSKAQILVVLAMLSVGLQSVSAAPIEGQFVEENDYQWSVQVTHCEPTIDMTKYPVIVGEKAVYLVLLDERDVQKGGGLCDYQPALMPVPKDYVEISGNKLSFKVNLEGQKLPFYCMYLGVDFPDKLVMVTIEELAKHSVRYDWGYISEPKPLTLSFLRKIVEAFESQVDNPFPHSAFKNEAVWNAVRDTPYIISGPVHVSAVDYGTDYVELEIEIPVRTDVTELTGTIEVKSGESTQTLSVTGSDVVNGVIKKREYRRPRIPWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS1</accession>
    <name>Q8TXS1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase fused to uncharacterized conserved domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiD</name>
        <name evidence="2" type="ordered locus">MK0589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0589"/>
    <dbReference type="EnsemblBacteria" id="AAM01804">
        <property type="protein sequence ID" value="AAM01804"/>
        <property type="gene ID" value="MK0589"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0589"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.624"/>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="OMA" id="AWIEIPA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="1">
        <location>
            <begin position="11"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="432" mass="46262" checksum="D528F49FD9FC57F6" modified="2002-06-01" version="1">MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLARYVPRCEVVKVGAVPTLKTFEILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGETVDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTGVLVTGGHSVDRRDVWVPAEGNTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRVADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGLSGRIVLDGDRPTAVGRPVPGGSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGFVVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEGDVGKEPMIRVLGKSAVEAVTKLRMLLGCVVGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-04-26" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXS2</accession>
    <name>PURP_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="2">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purP</name>
        <name type="ordered locus">MK0588</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + ATP + formate = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="2"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TXS2"/>
    <dbReference type="SMR" id="Q8TXS2"/>
    <dbReference type="STRING" id="190192.MK0588"/>
    <dbReference type="EnsemblBacteria" id="AAM01803">
        <property type="protein sequence ID" value="AAM01803"/>
        <property type="gene ID" value="MK0588"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0588"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.623"/>
    <dbReference type="HOGENOM" id="CLU_065084_0_0_2"/>
    <dbReference type="OMA" id="CIHYFYS"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="PTHR38147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148025" description="5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="116"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="146"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <sequence length="359" mass="40651" checksum="1F98F9E40CA7B11A" modified="2002-06-01" version="1">MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGVADEIIVVERFQDMLDEEVQERLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLRWESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGARGGRGYFICSDPEEFEEKAERLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMPAGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLCTEDLEFYVFELSARIDGGTNVTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXT5</accession>
    <name>Q8TXT5_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0575"/>
    <dbReference type="EnsemblBacteria" id="AAM01790">
        <property type="protein sequence ID" value="AAM01790"/>
        <property type="gene ID" value="MK0575"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0575"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.610"/>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="OMA" id="FYYKLGK"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="235" mass="25509" checksum="97FCEBBABF16ED5A" modified="2002-06-01" version="1">MPICVEEPERIREAAESLMETLDLDLYPVAMKLYLSEGDVPDDYERVGEECRHCEFVMTVARGEMDNIYATPEEQACKGGSAAIGLDDIPDPVRTGKFYYEGLGQHATLSASKRTVELVPKAFDGEGVFEAITYEKATGVEVVPDVILVVCKPKEAMKIAQAYLYPEGGRVYSDFSGIQSLCGDGVGKIMKEGGINFTLGCNGSRAYAKVPDECLIVAMSTKAFVVVGHSVTEIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXU4</accession>
    <name>FEN_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flap endonuclease 1</fullName>
            <shortName evidence="2">FEN-1</shortName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fen</name>
        <name type="ordered locus">MK0566</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
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        <property type="protein sequence ID" value="AAM01781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="4WA8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-348"/>
    </dbReference>
    <dbReference type="PDBsum" id="4WA8"/>
    <dbReference type="AlphaFoldDB" id="Q8TXU4"/>
    <dbReference type="SMR" id="Q8TXU4"/>
    <dbReference type="STRING" id="190192.MK0566"/>
    <dbReference type="EnsemblBacteria" id="AAM01781">
        <property type="protein sequence ID" value="AAM01781"/>
        <property type="gene ID" value="MK0566"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0566"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.601"/>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="OMA" id="GSQDYDS"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_structure-sp_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="PTHR11081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154054" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="113"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="2">
        <location>
            <begin position="340"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="17"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="24"/>
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        </location>
    </feature>
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        <location>
            <begin position="29"/>
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    <feature type="helix" evidence="3">
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            <begin position="56"/>
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        </location>
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    <feature type="strand" evidence="3">
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        </location>
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    <feature type="helix" evidence="3">
        <location>
            <begin position="126"/>
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        </location>
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    <feature type="strand" evidence="3">
        <location>
            <begin position="143"/>
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        </location>
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    <feature type="helix" evidence="3">
        <location>
            <begin position="150"/>
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    <feature type="strand" evidence="3">
        <location>
            <begin position="162"/>
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        </location>
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    <feature type="strand" evidence="3">
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        </location>
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            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="179"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
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        </location>
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        <location>
            <begin position="194"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
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        </location>
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    <feature type="strand" evidence="3">
        <location>
            <begin position="205"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="209"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="221"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="245"/>
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        </location>
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    <feature type="helix" evidence="3">
        <location>
            <begin position="259"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="267"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="278"/>
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        </location>
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    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="318"/>
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        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4WA8"/>
        </source>
    </evidence>
    <sequence length="348" mass="40252" checksum="2058C441900B7AF9" modified="2002-06-01" version="1">MGLAELRELIEPEETDLRALAGREIAIDAFNALYQFLTTIMKDGRPLMDSRGRITSHLNGLLYRTVNLVEEGIKPVYVFDGEPPDLKRETLERRRERKEEAMEKLRRAKTKEEREKYARQVARLDESLVEDAKRLLDLMGIPWVQAPSEGEAQCAYMARCGDVWATGSQDYDSLLFGSPRLVRNITIVGKRKHPHTGEIIEVKPEIMRLEDVLDQLGLESREQLVDLAILLGTDYNPDGVPGIGPKRALQLIRKYGSLDELKDTDIWPKIERHLPVEPEKLRRLFLEPEVTDDYELDWDEPDEEGLVEFLVEERDFSEDRVRRAVERLKEALQELRKGGRQETLDAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TXY1</accession>
    <name>Q8TXY1_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent DNA ligase, homolog of eukaryotic ligase III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAM01743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0528"/>
    <dbReference type="EnsemblBacteria" id="AAM01743">
        <property type="protein sequence ID" value="AAM01743"/>
        <property type="gene ID" value="MK0528"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0528"/>
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    <dbReference type="HOGENOM" id="CLU_061502_0_0_2"/>
    <dbReference type="OMA" id="ICPFTTK"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01209">
        <property type="entry name" value="TIGR01209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA_ligase" evidence="1">
        <location>
            <begin position="92"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Lig_C" evidence="2">
        <location>
            <begin position="259"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
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            <dbReference type="Pfam" id="PF18330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="386" mass="45140" checksum="8E3B1E66BFE7F655" modified="2002-06-01" version="1">MADPSKDVKKLSMILESEEWRVEKAIERGTIRRVRTSFPGFVYYRTVREFAGFERGTLIVPSHGLLLRGFPKIERALLLEPALRSRFGEVGKVIVEEKMNGHNVRVFELDGEVYAATRGGLICPYTTHKLRNMFEDELKQFFEDYPGTVVCGEFVGKENPYVSREYPEARDVGFFVFDVRDPSGRFWSYEEKLKVDEYGLERVRCFGEFEVSEVEEIHRIVRELDREGREGIVIKDPDRKLPPLKYTTHSAHVEEIEWAFRFAFDLGRDFVLTRTIREGFQSFEWCEKDRELKRRGAEIGRAIVEGLRRAVEQVVEEGEAYEEIPLTFESREWFERYEDFVKRVTGGTHSLKIIEEKEDGTVRAVLRKRYFGTGDKVSRILEEGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY05</accession>
    <name>Q8TY05_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein conserved in archaea</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0502</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01717">
        <property type="protein sequence ID" value="AAM01717"/>
        <property type="gene ID" value="MK0502"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0502"/>
    <dbReference type="HOGENOM" id="CLU_074023_0_0_2"/>
    <dbReference type="OMA" id="WNGRETL"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="314" mass="34986" checksum="A7E6E4983F6D2F78" modified="2002-06-01" version="1">MEPIFQTLLALLIATQTGVEHDTGCCTVLVHVHHGCDVVAYRRDAKFPAEILIVRTEWAGRRAIKEYKIQGGYFCHTVITEDGWIVTIGGRDIPEVNRKLEKLGAEIVSKGRIKKDEIEKAGELLKEARWGHFVVKSPNDIVGVASYDYRISSPERIDMFKIKDGEYVKVTNNPRYYDRGRSEEFGKNPIDAAIKIAGKDPYGLHRRDIITYKLTVDETSSSVKVWASYDGRALLGGASGEPDPIRFMGKTIAANEIPRVPRRKPLGEVILRVCREHEGKGELPNPAAVAAALISGAVFVAFLGQLSLQQRNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY53</accession>
    <name>Q8TY53_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">MK0453</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0453"/>
    <dbReference type="EnsemblBacteria" id="AAM01668">
        <property type="protein sequence ID" value="AAM01668"/>
        <property type="gene ID" value="MK0453"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0453"/>
    <dbReference type="HOGENOM" id="CLU_068443_2_0_2"/>
    <dbReference type="OMA" id="CNKELFA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026170">
        <property type="entry name" value="FAM173A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13610">
        <property type="entry name" value="PTHR13610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="4">
        <location>
            <begin position="59"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AAM01668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="182" mass="20654" checksum="D0349BD2C9754834" modified="2002-06-01" version="1">MIGKRKVERLLDEFPNEVEYHRSDDHVVTCPTELYLVPTPIEIVVEILRKLELTPKNRVVDLGCGDGRFVISAAYLYGCEGMGVDVREDVLELARAKSETLRVDDKTIFIHSDVRDIDLRELNPDVVFVYLMPSLLEEISEELVSCGATVVSYTFEVPGLGVPEVLRLDDLRRAYVYRGVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY60</accession>
    <name>Q8TY60_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadC</name>
        <name evidence="9" type="ordered locus">MK0446</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>CO2 + diphosphate + nicotinate beta-D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0446"/>
    <dbReference type="EnsemblBacteria" id="AAM01661">
        <property type="protein sequence ID" value="AAM01661"/>
        <property type="gene ID" value="MK0446"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0446"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.475"/>
    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
    <dbReference type="OMA" id="DMIMLKD"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="QRPTase_N" evidence="8">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="QRPTase_C" evidence="7">
        <location>
            <begin position="107"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="282" mass="30553" checksum="7A97EE8845292629" modified="2002-06-01" version="1">MLLNEVKVRKIVELALTEDVGRADLTSSIVEGERAEAEIVAKEEGVVSGTLPARLTFELLDCEVDVLVEDGERIQPGDIVLKAYGEATALLAAERVALNFLMRLSGIATATRKVVERVREVNPDVIVAATRKVHPITGFLEKKAVSDGGGDLHRFGLDDAVLIKDNHLALVGSVRDAVRRARERVGFTKVIGVEVESIEDAVEAAKAGADHVLLDNMKPAEIRRAVNEVRKVREDVILEASGGITPENAPEYAETGVDVISLGWLTHSAPALDLSMRVRRMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY83</accession>
    <name>Q8TY83_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01637">
        <property type="protein sequence ID" value="AAM01637"/>
        <property type="gene ID" value="MK0422"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0422"/>
    <dbReference type="HOGENOM" id="CLU_1438118_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="20066" checksum="DFD8A831F6E1931E" modified="2002-06-01" version="1">MVLRATLTPLHALIFGLTLFTAFIRPPLAPIIYEKRERYDPWNSIVLAALMIYGLAPLVGLENSCLAFLVAKGVDGAGGLAQFIPGSINPVVSLKGFRIREVEEGISPIGTFFEFLAALGLGWLITGSTQGALVMGIAVTAADILSNVTGVEDHHKGDDLLMMLSAAWVIQTFGVSGLMRLLGLMPGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY88</accession>
    <name>Q8TY88_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L44e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl42A</name>
        <name evidence="2" type="synonym">rpl44e</name>
        <name evidence="4" type="ordered locus">MK0417</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0417"/>
    <dbReference type="EnsemblBacteria" id="AAM01632">
        <property type="protein sequence ID" value="AAM01632"/>
        <property type="gene ID" value="MK0417"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0417"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.446"/>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="OMA" id="CKKHTIH"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_L44e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="PTHR10369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 3 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AAM01632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="93" mass="10918" checksum="872FB7823CD52E68" modified="2002-06-01" version="1">MAKIPKKIRTYCPYCRKHTIHEVERAKKNPARKMSWGQRQFERVLKGYGGFPRPKPSGEKPTKKVDLRYRCTECGKAHTRKGWRAGTLEITEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TY92</accession>
    <name>RL23_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl23</name>
        <name type="ordered locus">MK0413</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TY92"/>
    <dbReference type="SMR" id="Q8TY92"/>
    <dbReference type="STRING" id="190192.MK0413"/>
    <dbReference type="PRIDE" id="Q8TY92"/>
    <dbReference type="EnsemblBacteria" id="AAM01628">
        <property type="protein sequence ID" value="AAM01628"/>
        <property type="gene ID" value="MK0413"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0413"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.442"/>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="OMA" id="MDPYKVI"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_L23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_L23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000272943" description="50S ribosomal protein L23">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <sequence length="95" mass="10856" checksum="EA75209EA1C26A05" modified="2002-06-01" version="1">MAKRYGPKIEDPHDVLLYPVATEKAMRLMEAENKLTFIVRRDANKPLIKKAVEELFDVEVEKVNTLITPTGEKKAYVKLKPEYRAEDVAVDLGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYA9</accession>
    <name>Q8TYA9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase, beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">comE</name>
        <name evidence="2" type="ordered locus">MK0393</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0393"/>
    <dbReference type="EnsemblBacteria" id="AAM01608">
        <property type="protein sequence ID" value="AAM01608"/>
        <property type="gene ID" value="MK0393"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0393"/>
    <dbReference type="HOGENOM" id="CLU_117492_1_0_2"/>
    <dbReference type="OMA" id="GSVLMNM"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd03372">
        <property type="entry name" value="TPP_ComE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022494">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03846">
        <property type="entry name" value="sulfopy_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="1">
        <location>
            <begin position="39"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="188" mass="20755" checksum="0B1889AD6D495802" modified="2002-06-01" version="1">MGMKRIEALRVVADVAERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGLALCTDREVLAIEGDGGLMMNMGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRIDWEKVAEAHGLTYFEASEPESAEVALEDALATEGPRMVRLEVEPGNADVPLIDLDPEEIKVRFVRALREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYC9</accession>
    <name>Q8TYC9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted GTPase, probable translation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q8TYC9"/>
    <dbReference type="STRING" id="190192.MK0373"/>
    <dbReference type="EnsemblBacteria" id="AAM01588">
        <property type="protein sequence ID" value="AAM01588"/>
        <property type="gene ID" value="MK0373"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0373"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.398"/>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="OMA" id="QIDCACA"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="MMR_HSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="MMR_HSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="2">
        <location>
            <begin position="3"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="402" mass="44508" checksum="D7D531395D736894" modified="2002-06-01" version="1">MGVQVGIVGKPNVGKSTFFAAATLSPVETADYPFTTVDPNQGVAHVRTECPCKAFGVECQPRNSSCIDGNRFVPVELIDVAGLVPGAHEGRGLGNKFLDDLRQASVLIHVVDVSGSTDEEGRPCDPGTREPAEDVRFLERELDEWIAGILRRDWDRTAKRASLEKIPAAEVLRERLAGIGVSASDVESAMERAEVPSDLTKWSDEDIRRFAREVRRVNKPMVIAANKIDVECAEENLKRLEEEVEYPVVPTCAEAELALRRAADQGLIRYLPGDSDFEILQEEKLSDEQLQALEFIREKVLKRWGSTGVQEALNRAVFDVAKMIVVYPVENENKLSDSEGRVLPDALLLPEGTMVRELAYNIHTEIGESFNRAILVKPDGSREVVGEDHELEHGDVVKIQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYF8</accession>
    <name>Q8TYF8_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted transcriptional regulator containing DNA-binding HTH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01557">
        <property type="protein sequence ID" value="AAM01557"/>
        <property type="gene ID" value="MK0342"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0342"/>
    <dbReference type="HOGENOM" id="CLU_2565764_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r4_2" evidence="1">
        <location>
            <begin position="27"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="81" mass="9431" checksum="679373C9E8AB48A9" modified="2002-06-01" version="1">MAEQKPYYGAWPCHPRDLVEEGVTTSARRARIVLRKYVLGQSEEEIAEEEDISLKSVKYHLEKAEEEGLIDYVKEKFVESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG0</accession>
    <name>Q8TYG0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">MK0339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01554">
        <property type="protein sequence ID" value="AAM01554"/>
        <property type="gene ID" value="MK0339"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0339"/>
    <dbReference type="HOGENOM" id="CLU_709059_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="389" mass="42598" checksum="6FF59E8C11D042D9" modified="2002-06-01" version="1">MFPRTLIPLISAILLATPACATDVGVFAWGSTIRSEGVQKFTDDILSTGYTEVAVLIRGVSTPTRVDTLSAVYSQIKARNPNVKVYAWIVGFERKDGWDKPWDPEVRREILDAVRSALPYCDGIILDDSFRYPTSNPVKREKAMQAITDLVREISELAHSHGKAVYFCLLPEKPEPYSIDRDAIATYVDKFIVEAYTEEYGRDDEWPVRVYNLYERLYPGKVAIALHELNESRLAHQLTLLKEAGCPDIWLFRYGEVKKMGLVRDVIRSITGSASGTSLQWSPVEGGQFDLAGLLLTGLVLGTFGGLVLAIVNPELLNSLGQAAIGIAETAQELLQGAFEAVSGALSGIQEKVLGASAEGDLGPIIGILARVALVFVWGWLLATLLSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYG9</accession>
    <name>Q8TYG9_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized secreted protein specific for M.kandleri, MK-30 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01545">
        <property type="protein sequence ID" value="AAM01545"/>
        <property type="gene ID" value="MK0330"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0330"/>
    <dbReference type="HOGENOM" id="CLU_870477_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="319" mass="34612" checksum="4599EF5B8E1CB341" modified="2002-06-01" version="1">MLGAALAAILLVLPGSALADAWSPVSKVHLTERTELTLTDQNTVRAHLTVEVLGYGLEGSTRVPIKVLVPSDAELVTAKWKPERGGEVEVEPVSVRTVTWPGNRDQIEWVTYNLHSGSTPYMDALEALFPNKMIRWRELTFVLPVEGTGGYDEQLGTLEVVTTCRTHEGEQGTYFAWPIPAAANPAYEVRVHGLSVGFVAVYDPETGRWRWIVPGEWSYSAPPSSRSANVNTVIVDVAGLPFGLSVEAGGVRVDPGPTWKASRFLSLTRYLPVLVVMEISPVGPAPQRGETASERGTTESTGGIVPTPLIVPIVPVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYJ0</accession>
    <name>Q8TYJ0_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable formylmethanofuran dehydrogenase subunit F, ferredoxin containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fwdF_1</name>
        <name evidence="2" type="ordered locus">MK0307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
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    <dbReference type="HOGENOM" id="CLU_050974_0_0_2"/>
    <dbReference type="OMA" id="VYCKVCE"/>
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    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
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        <property type="entry name" value="MvhB-like"/>
    </dbReference>
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        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="27"/>
            <end position="56"/>
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            <end position="94"/>
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            <end position="137"/>
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            <end position="176"/>
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    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="266"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AAM01524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="357" mass="38003" checksum="A7F4198B3B52600E" modified="2002-06-01" version="1">MAEVRLEPVKEVSPPLLRVVRKGVEERELVINTSKCAACGICEAACPVGAISVAPPSAVVRKGEDPIDVDESKCVLCGICAFVCPYDVIQLLVNGKPMTEAGLPNLPKSVEVDEEECVYCSLCADTCPQEAITVEREVPTPGDLVMGEIEIDEEKCIYCKACEEACPADAITVERPKPSAADPEPEFTIEVDEDKCVYCGVCMRTCPVDAIKVGCMVCYGTTRGEIPAKGEVDGSVDVDPSSCVYCGWCGFVCPVDAIEVIKPYEGTLEVDDEECVGCGLCVEVCPCGALEFEKGGKAGKTKIVAHPETCAYCGACARACPVNAITVVREGVTAMPELPTKAWKKALDRVLGPVEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYQ7</accession>
    <name>Q8TYQ7_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Adenylosuccinate synthetase</fullName>
            <shortName evidence="7">AMPSase</shortName>
            <shortName evidence="7">AdSS</shortName>
            <ecNumber evidence="7 8">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">purA</name>
        <name evidence="9" type="ordered locus">MK0235</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
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                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
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            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="7 8"/>
    <dbReference type="EMBL" id="AE009439">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAM01452">
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        <property type="gene ID" value="MK0235"/>
    </dbReference>
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        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 7 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="IMP binding" evidence="7">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="IMP binding" evidence="7">
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            <end position="47"/>
        </location>
    </feature>
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            <begin position="262"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
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            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
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            <position position="47"/>
        </location>
    </feature>
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        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
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        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
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        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
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        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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            <end position="296"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AAM01452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="346" mass="37631" checksum="73C8126BF0CF1196" modified="2002-06-01" version="1">MRDRGHVSVVAGGQWGDEGKGKIVAYLAVQDEPEVIARAGVGPNAGHTVRVNGEDYGLRQIPCGFPHEEAELAIGPGVLVNPEVLLDEVERLSRFRVDDRLIVDERCAIIEPKHIEAERASKHLSDEIDTTGTGCGPANADRALRKAKLARDVDELSEFLGDVPGLVNEAIDAGEDVLIEGTQGFGLSLYHGIDYPYVTSKDTTASAFASDVGVGPTRIDDVYVVFKAYATRVGEGPFPTELSREEVIEKFGEEILEVERGTVTGRPRRIGEFDFEMAKRACVINGATQVAITCIDRRFPDAAEAETWHELPTEAKKFVEKVEEAVGVPVTIVSTGPELEHTVDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYR6</accession>
    <name>Q8TYR6_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase maturation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hypD</name>
        <name evidence="3" type="ordered locus">MK0226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0226"/>
    <dbReference type="EnsemblBacteria" id="AAM01443">
        <property type="protein sequence ID" value="AAM01443"/>
        <property type="gene ID" value="MK0226"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0226"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.227"/>
    <dbReference type="HOGENOM" id="CLU_048562_1_0_2"/>
    <dbReference type="OMA" id="HVCGSHE"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11750">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="PTHR30149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="350" mass="38466" checksum="86EE89E26E206F19" modified="2002-06-01" version="1">MKMTRREFVRKLVRRIHKEAETLDRDVTIMHVCGSHERTIVEHGLRSLLPENVRLVCGPGCPVCVTTTGELAAAIKAAEDGMVVCAFGDVYRVPTPVGSLSSCDGDVRVVQSVRKAEEIAESEDRDVLYLAVGFETTAPTTAAVLLDDPPSNFYVLSAHRLIPPAMEWLLESGECRLDAFICPGHVSTIIGTGPYESVARELPCVVAGFEPEDVLIAVLACLKMLRRGRVGVTNEYLRAVEDRGNPVAKELMERAFEPEDRPWRGFPTIPESALKLREDLADHDAIEIGIRPDYTLGHDESCICDRILRGLAEPRDCPLFGTKCTPTDPVGPCMVSEEGPCFIEYRFGGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYT4</accession>
    <name>SECG_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">secG</name>
        <name type="ordered locus">MK0208</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. Can form oligomers of the heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYT4"/>
    <dbReference type="EnsemblBacteria" id="AAM01425">
        <property type="protein sequence ID" value="AAM01425"/>
        <property type="gene ID" value="MK0208"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0208"/>
    <dbReference type="HOGENOM" id="CLU_2985689_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000157269" description="Preprotein translocase subunit SecG">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="57" mass="6293" checksum="267759717B2638C8" modified="2002-06-01" version="1">MGKKMEKKRAEMPPARAGILSFWDEEAPGIKIDPDYILYACFAVAVLLIIAHTMAAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-12-21" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYV9</accession>
    <name>Y182_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter ATP-binding protein MK0182</fullName>
            <ecNumber>7.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MK0182</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TYV9"/>
    <dbReference type="SMR" id="Q8TYV9"/>
    <dbReference type="STRING" id="190192.MK0182"/>
    <dbReference type="PRIDE" id="Q8TYV9"/>
    <dbReference type="EnsemblBacteria" id="AAM01399">
        <property type="protein sequence ID" value="AAM01399"/>
        <property type="gene ID" value="MK0182"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0182"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.182"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092143" description="Putative ABC transporter ATP-binding protein MK0182">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="268" mass="29422" checksum="1AE01BACDC91E3C9" modified="2002-06-01" version="1">MTHEYPDGTCAVCGLSLRVKEGESVVVLGPNGSGKTTLLHHILGLLTPTKGHIRVLGHDLPDGVREVRKRIGVVFQDVDDQLIMPTVLEDVAFGLVNRGMPREEAFERAREILERLGIEDLEDRPPQFLSGGQKRLVALAGAVAPEPDLLILDEPTSGLDFRATRLFVRLIRELKEELGFTMILTTFDVDIAAALAERVVVIREGKTVAEGSPEDILTDVDLIRESGLKPPEHVELLRRLGIENPPLDISEAEELLVAMLGEESRGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TYY2</accession>
    <name>Q8TYY2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MK0159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAM01376">
        <property type="protein sequence ID" value="AAM01376"/>
        <property type="gene ID" value="MK0159"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0159"/>
    <dbReference type="HOGENOM" id="CLU_1010526_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AAM01376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="275" mass="27779" checksum="2D6A1E104A0D13A9" modified="2002-06-01" version="1">MKLSLKSTILVMLGMCVAYSALVGSITIAQTGQSPGAPVGQPTEPTGQGKKPPVTISKLVIEVQATGKGAQKPRESDIGISGAKVEDVKISGSRAYAKIVASNVSLEEGFPLMVNAGWPFNMSECSYERISVSMELKANVNSAPETELSGVVVLADGRLVDADPKPARVNDNVPVWKIGVKDGRVTIDGRPYGPFPEIRYVMEYSRGAGGPGAGAEGGGAGGAAGGAGVGGAGGAGAGGAVTAGQYKAGESLFLIAAVVLAAVVGLYLVYRGLSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2005-08-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ45</accession>
    <name>ASPD_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="1">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadX</name>
        <name type="ordered locus">MK0094</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="1"/>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZ45"/>
    <dbReference type="SMR" id="Q8TZ45"/>
    <dbReference type="STRING" id="190192.MK0094"/>
    <dbReference type="EnsemblBacteria" id="AAM01311">
        <property type="protein sequence ID" value="AAM01311"/>
        <property type="gene ID" value="MK0094"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0094"/>
    <dbReference type="HOGENOM" id="CLU_089550_0_0_2"/>
    <dbReference type="OMA" id="RARGNQH"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="DUF108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000144897" description="L-aspartate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <sequence length="262" mass="28185" checksum="298438D77FC7BD75" modified="2002-06-01" version="1">MKKLSLALVGAGGIGTTVLREIREGRLEGKVEPVLVCDRHPEKLKRIERWFPDCDTSTDLDDAMSAEADVLLEAASVEAAASLLPDALKRFDVIVMSVGALVLEEGLLSRCREVAEVTGHRLHVPSGAVGGLDVLRALRGRVREVTLTTIKPPKALNKDVSERTVLYEGSVRDAVRKFPKNINVAAAVSLAVGDPSLVTVRIVCDPEVSVNTHVIEVESSAGTYRFELRNEALPDNPKTSAVAAYSAVALIERMTEGIRVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZ79</accession>
    <name>Q8TZ79_METKA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component Csl4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">csl4</name>
        <name evidence="6" type="ordered locus">MK0060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the exosome, which is a complex involved in RNA degradation. Increases the RNA binding and the efficiency of RNA degradation. Helpful for the interaction of the exosome with A-poor RNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the archaeal exosome complex. Forms a trimer of Rrp4 and/or Csl4 subunits. The trimer associates with an hexameric ring-like arrangement composed of 3 Rrp41-Rrp42 heterodimers. Interacts with DnaG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CSL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0060"/>
    <dbReference type="EnsemblBacteria" id="AAM01277">
        <property type="protein sequence ID" value="AAM01277"/>
        <property type="gene ID" value="MK0060"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0060"/>
    <dbReference type="HOGENOM" id="CLU_067135_1_1_2"/>
    <dbReference type="OMA" id="CGNVETR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00975">
        <property type="entry name" value="Exosome_Csl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030850">
        <property type="entry name" value="Exosome_Csl4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="PTHR12686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0271">Exosome</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="68"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AAM01277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="196" mass="21612" checksum="7E63D66A6BED2F9D" modified="2002-06-01" version="1">MRRYVEPGKFVLPGDKITVAEAFYPGPGTYEDGGVVRAAITGRVEVDLEEREVRVEPYVDTPPKLKRGASVIGRVQSVKEQVVLVKICFVDDRTDREPPTSGVGGIHISKVRDAYVEDLADEFQPGDIVRARVVSTKMPVQLSTVGKEYGVVLAYCTRCRSELEKVKGRTLRCPVCGHTETRKVAAGYLREAESDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2002-06-01" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZA2</accession>
    <name>Q8TZA2_METKA</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized conserved protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">MK0034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="190192.MK0034"/>
    <dbReference type="EnsemblBacteria" id="AAM01251">
        <property type="protein sequence ID" value="AAM01251"/>
        <property type="gene ID" value="MK0034"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0034"/>
    <dbReference type="HOGENOM" id="CLU_2103513_0_0_2"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AAM01251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000001826"/>
        </source>
    </evidence>
    <sequence length="115" mass="12980" checksum="539FD7AA924CE003" modified="2002-06-01" version="1">MLGFLKKIFGEGENSKGPMLEDFLEEEEEERTQHPKVTIIVSRVQEPSDLEELMNELYQGNVLILDVKPLLDRDGHEDIIQELKRTAVSLGGFVGVIKDTVLLVTSDSVDIERRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q8TZC4</accession>
    <name>RFCS_METKA</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor C small subunit</fullName>
            <shortName>RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Clamp loader small subunit</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mkn RFC intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">rfcS</name>
        <name type="ordered locus">MK0006</name>
    </gene>
    <organism>
        <name type="scientific">Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)</name>
        <dbReference type="NCBI Taxonomy" id="190192"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanopyri</taxon>
            <taxon>Methanopyrales</taxon>
            <taxon>Methanopyraceae</taxon>
            <taxon>Methanopyrus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="4644" last="4649">
            <title>The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.</title>
            <authorList>
                <person name="Slesarev A.I."/>
                <person name="Mezhevaya K.V."/>
                <person name="Makarova K.S."/>
                <person name="Polushin N.N."/>
                <person name="Shcherbinina O.V."/>
                <person name="Shakhova V.V."/>
                <person name="Belova G.I."/>
                <person name="Aravind L."/>
                <person name="Natale D.A."/>
                <person name="Rogozin I.B."/>
                <person name="Tatusov R.L."/>
                <person name="Wolf Y.I."/>
                <person name="Stetter K.O."/>
                <person name="Malykh A.G."/>
                <person name="Koonin E.V."/>
                <person name="Kozyavkin S.A."/>
            </authorList>
            <dbReference type="PubMed" id="11930014"/>
            <dbReference type="DOI" id="10.1073/pnas.032671499"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>AV19 / DSM 6324 / JCM 9639 / NBRC 100938</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="AE009439">
        <property type="protein sequence ID" value="AAM01223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q8TZC4"/>
    <dbReference type="SMR" id="Q8TZC4"/>
    <dbReference type="STRING" id="190192.MK0006"/>
    <dbReference type="MEROPS" id="N10.007"/>
    <dbReference type="EnsemblBacteria" id="AAM01223">
        <property type="protein sequence ID" value="AAM01223"/>
        <property type="gene ID" value="MK0006"/>
    </dbReference>
    <dbReference type="KEGG" id="mka:MK0006"/>
    <dbReference type="PATRIC" id="fig|190192.8.peg.6"/>
    <dbReference type="HOGENOM" id="CLU_015698_2_0_2"/>
    <dbReference type="OMA" id="NWRYEFR"/>
    <dbReference type="Proteomes" id="UP000001826">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000030368" description="Replication factor C small subunit, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030369" description="Mkn RFC intein" evidence="2">
        <location>
            <begin position="83"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030370" description="Replication factor C small subunit, 2nd part" evidence="2">
        <location>
            <begin position="388"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="635" mass="71586" checksum="9AC69359719E08C5" modified="2002-06-01" version="1"> </sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>